FastQCFastQC Report
Thu 26 May 2016
SRR938923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938923_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319108
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62091.9457362397683542No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43921.3763365380999535No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27200.852375998094689No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26440.8285596099126314No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23270.7292202013111548No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22190.6953758602103363No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA21400.670619351442145No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20300.6361482632839038No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20290.6358348897551925No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC19870.6226732015493187No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17590.5512240370031463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16670.5223936723617082No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC15710.4923098136054251No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15630.4898028253757349No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15560.4876092106747559No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA13850.43402233726512657No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA13060.40926582849693516No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC11450.35881269037441865No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC11240.35223184627148174No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT11110.3481579903982351No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10400.325908469859734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9390.29425774345989447No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT9320.29206412875891546No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG9170.28736352582824626No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA8590.26918786116299187No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC8390.2629203905887662No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG7200.22562894067212355No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT7020.21998821715532046No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC6880.2156009877533625No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG5700.17862291136543113No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT5640.17674267019316345RNA PCR Primer, Index 26 (100% over 22bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT5530.17329556137733934No Hit
GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC5260.1648344761021347No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.13663085851811926No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG4300.13475061734585156No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG4010.12566278501322437No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG3960.12409591736966795No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT3880.12158892913997768No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA3860.12096218208255513No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT3560.11156097622121665No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC3520.11030748210637152No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT3350.10498013211827971No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT3280.10278651741730072No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA3250.10184639683116688No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.10153302330245559No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT3230.10121964977374431RNA PCR Primer, Index 26 (100% over 24bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG3200.10027952918761046No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGCG352.7989235E-454.230479
GTAAGCG402.2786298E-741.52020394-95
GGATATG1051.033186E-940.672857
CTCCGAC652.0736479E-1036.50127894-95
GTCTTGA600.003893926331.7538531
CTGGTCG902.4243565E-531.634449
CATATAG1401.6529157E-830.6197871
CTAGCCA804.5132803E-429.6572864
ATATGAG1503.3536708E-828.4709959
GTGTAGG856.2948844E-428.0181061
GTGTAGA856.2948844E-428.0181061
CTCCCGC856.4351596E-427.9127437
GTACCCA700.00827092627.2175881
GTCGCTC350.00843058727.11523426-27
ACTCCCG908.9865935E-426.3620346
CCCGCTG908.9865935E-426.3620349
GATATGA1701.128592E-725.1214688
GGTACTC1000.001660851323.725833
CGCAGAA1401.788296E-523.725839
GAGTACT12550.023.44960412-13
Produced by FastQC (version 0.11.5)