##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938923_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 319108 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09263634882234 33.0 31.0 34.0 30.0 34.0 2 32.33903882071274 34.0 31.0 34.0 30.0 34.0 3 32.4029764217757 34.0 31.0 34.0 30.0 34.0 4 35.958208506211065 37.0 35.0 37.0 35.0 37.0 5 35.882704288203364 37.0 35.0 37.0 35.0 37.0 6 35.94914261002545 37.0 35.0 37.0 35.0 37.0 7 35.905035285859334 37.0 35.0 37.0 35.0 37.0 8 35.89743911152337 37.0 35.0 37.0 35.0 37.0 9 37.52201762412726 39.0 37.0 39.0 35.0 39.0 10-11 37.441258132043075 39.0 37.0 39.0 34.0 39.0 12-13 37.38892945335122 39.0 37.0 39.0 34.0 39.0 14-15 38.595862529300426 40.0 38.0 41.0 34.0 41.0 16-17 38.6869351442145 40.0 38.0 41.0 34.0 41.0 18-19 38.720345462978045 40.0 38.0 41.0 34.0 41.0 20-21 38.71049926670594 40.0 38.0 41.0 34.0 41.0 22-23 38.63558105719693 40.0 38.0 41.0 34.0 41.0 24-25 38.519598380485604 40.0 38.0 41.0 34.0 41.0 26-27 38.32921612745528 40.0 38.0 41.0 34.0 41.0 28-29 38.18074601702245 40.0 38.0 41.0 33.0 41.0 30-31 38.0249366985472 40.0 37.0 41.0 33.0 41.0 32-33 37.84947102548354 40.0 37.0 41.0 33.0 41.0 34-35 37.7194084761272 40.0 37.0 41.0 33.0 41.0 36-37 37.575822292139335 40.0 36.5 41.0 33.0 41.0 38-39 37.39255831881369 40.0 36.0 41.0 32.5 41.0 40-41 37.298251689083315 40.0 36.0 41.0 32.0 41.0 42-43 37.08961699487321 40.0 36.0 41.0 31.0 41.0 44-45 36.851211815435526 40.0 36.0 41.0 31.0 41.0 46-47 36.521958083156804 40.0 36.0 41.0 30.0 41.0 48-49 36.166145944319794 39.0 35.0 41.0 29.5 41.0 50-51 35.938779347430966 39.0 35.0 41.0 28.5 41.0 52-53 35.73457418804919 39.0 35.0 40.0 28.0 41.0 54-55 35.50256809606779 39.0 35.0 40.0 27.5 41.0 56-57 35.361658122015115 39.0 35.0 40.0 27.5 41.0 58-59 35.10734296852476 38.0 35.0 40.0 26.5 41.0 60-61 34.671924552189225 38.0 34.0 40.0 26.0 41.0 62-63 34.25996371134538 38.0 34.0 40.0 25.0 41.0 64-65 33.84005258407812 37.0 33.5 40.0 23.0 41.0 66-67 33.307496208180304 37.0 33.0 39.0 22.0 40.5 68-69 32.795671058074376 36.0 32.0 39.0 20.5 40.0 70-71 32.20175927899019 35.5 31.5 38.5 18.5 40.0 72-73 31.65950869298169 35.0 31.0 37.5 16.0 39.5 74-75 31.150774972736503 35.0 31.0 37.0 10.5 39.0 76-77 29.181670468932147 32.5 28.5 35.0 7.5 37.5 78-79 30.152890244055303 34.0 29.5 36.0 7.0 38.0 80-81 30.087321533775402 34.0 30.0 36.0 4.5 37.5 82-83 29.647849630845982 34.0 29.5 35.0 2.0 37.0 84-85 29.15237474460057 34.0 29.0 35.0 2.0 36.5 86-87 28.51430080098274 34.0 29.0 35.0 2.0 36.0 88-89 27.79374537774045 33.0 26.5 35.0 2.0 35.5 90-91 27.199882171553206 33.0 26.0 35.0 2.0 35.0 92-93 26.826912832019254 33.0 25.0 34.5 2.0 35.0 94-95 26.27327895258032 32.0 25.0 34.0 2.0 35.0 96-97 25.419604021209118 32.0 22.0 34.0 2.0 35.0 98-99 24.267924965842287 32.0 10.0 34.0 2.0 35.0 100-101 18.77706920541008 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17184864879224904 1101 2 0.11259295140432357 1101 3 0.16382819240047297 1101 4 0.19829388328870579 1101 5 -0.00529937467904773 1101 6 0.06692579692719391 1101 7 0.20178857595787036 1101 8 0.15012233385097318 1101 9 0.4007194810264423 1101 10-11 0.34546192709924384 1101 12-13 0.2782946639574533 1101 14-15 0.39956398698644335 1101 16-17 0.4592287216028126 1101 18-19 0.5177290435880479 1101 20-21 0.35823049914964145 1101 22-23 0.42186450455812263 1101 24-25 0.6056957012992896 1101 26-27 0.5414874888214598 1101 28-29 0.3939989876321093 1101 30-31 0.22697234598488336 1101 32-33 0.19380972963984533 1101 34-35 0.19669186975036723 1101 36-37 0.48366173186519035 1101 38-39 0.5598894882444299 1101 40-41 0.698755738412558 1101 42-43 0.5010537717651999 1101 44-45 0.7934123123942811 1101 46-47 0.4857555527558972 1101 48-49 0.4124164482002328 1101 50-51 0.3781972632610504 1101 52-53 0.5484995056829334 1101 54-55 0.4848922187815248 1101 56-57 0.709873245286964 1101 58-59 0.6849932280908675 1101 60-61 0.4324350946921598 1101 62-63 0.9637714406970943 1101 64-65 0.7743996890769509 1101 66-67 0.6613646356458531 1101 68-69 0.9321349035512938 1101 70-71 0.9879541941719339 1101 72-73 0.866559941961281 1101 74-75 0.7061173667701013 1101 76-77 0.18953529961677518 1101 78-79 0.7249485517806917 1101 80-81 1.2730904538798633 1101 82-83 0.9862481612092431 1101 84-85 1.151241124265976 1101 86-87 1.4657685963644482 1101 88-89 1.184358627793312 1101 90-91 1.3273126735685992 1101 92-93 1.2591888648483298 1101 94-95 1.0019120970883826 1101 96-97 0.9954030824201965 1101 98-99 0.8234943537577948 1101 100-101 1.2457867521840882 1102 1 -0.05247201324467454 1102 2 -0.006384956500916417 1102 3 -0.12957563560543406 1102 4 0.1136518816916876 1102 5 0.051007640105481755 1102 6 -0.08583148039755173 1102 7 -0.027928074173999562 1102 8 -0.061639952305775125 1102 9 -0.3359417815863779 1102 10-11 -0.25459977590363536 1102 12-13 0.105090705286365 1102 14-15 -0.003876862821194038 1102 16-17 0.028348183455840115 1102 18-19 0.02516969981762429 1102 20-21 -0.020057303013594208 1102 22-23 -0.13444251022639975 1102 24-25 -0.068947994105649 1102 26-27 -0.24114251093656947 1102 28-29 0.00696992672761354 1102 30-31 -0.2757679932606436 1102 32-33 -0.10285467861626074 1102 34-35 -0.35468495411780054 1102 36-37 -0.4189927071214328 1102 38-39 -0.6636750095415209 1102 40-41 -0.30371843101662677 1102 42-43 -0.3380586728835695 1102 44-45 -0.561978836249466 1102 46-47 -0.838753847878067 1102 48-49 -0.8348371632402376 1102 50-51 -1.2002623394200072 1102 52-53 -1.0023328799245377 1102 54-55 -1.1924494706708941 1102 56-57 -1.0339927503091317 1102 58-59 -1.2222586895416754 1102 60-61 -1.1287113712985999 1102 62-63 -0.6433740090089088 1102 64-65 -0.8212944092122996 1102 66-67 -0.8443519660915086 1102 68-69 -0.9290817344819544 1102 70-71 -0.6671441676738361 1102 72-73 -0.38755964087802397 1102 74-75 -0.05798963349607078 1102 76-77 -0.5141269550835368 1102 78-79 -0.47161180827448135 1102 80-81 -0.5172403226100819 1102 82-83 -0.41926155398905607 1102 84-85 -0.2041621230057835 1102 86-87 -0.03980282183673012 1102 88-89 -0.11367292542855267 1102 90-91 -0.36740264571419345 1102 92-93 -0.2625705176376947 1102 94-95 -0.1758409457165655 1102 96-97 0.41834692058894873 1102 98-99 0.5891862742940077 1102 100-101 0.8542570016577997 1103 1 0.2209673383218984 1103 2 0.24694490082476506 1103 3 0.20976171093673202 1103 4 0.061571017113784876 1103 5 -0.0616505577425599 1103 6 0.021399405510393876 1103 7 0.12721246713518752 1103 8 0.17957917837630788 1103 9 -0.06431548400852449 1103 10-11 0.3631480081414864 1103 12-13 0.20941833480714678 1103 14-15 0.16946531853161417 1103 16-17 0.2450199851338013 1103 18-19 0.046156095987697654 1103 20-21 0.2652498496621476 1103 22-23 0.14431157803259254 1103 24-25 0.10075030423060838 1103 26-27 0.20806235231413694 1103 28-29 0.1107702300882849 1103 30-31 0.27634249638243347 1103 32-33 0.22986442836577226 1103 34-35 0.4169117183948856 1103 36-37 0.41607863003550705 1103 38-39 0.3947515439968967 1103 40-41 0.41988563591232264 1103 42-43 0.42568735735769536 1103 44-45 0.5111654496679634 1103 46-47 0.4769663967064659 1103 48-49 0.3305240255543822 1103 50-51 0.5242601045293682 1103 52-53 0.4170573177338994 1103 54-55 0.5054281896599591 1103 56-57 0.7408508293467406 1103 58-59 0.6785151171606998 1103 60-61 0.5596862681762076 1103 62-63 0.6814766806626125 1103 64-65 0.821279179449597 1103 66-67 0.7202543760424405 1103 68-69 0.7410964899101415 1103 70-71 0.8984101765457169 1103 72-73 0.46114515127251465 1103 74-75 0.8579877566541825 1103 76-77 0.7264641242168359 1103 78-79 0.8101818116714838 1103 80-81 1.1230521360333263 1103 82-83 0.883903587974256 1103 84-85 0.7405456553513297 1103 86-87 0.946178597322394 1103 88-89 0.892364662854142 1103 90-91 0.8716775552622487 1103 92-93 1.2024185803383212 1103 94-95 1.538757101399142 1103 96-97 1.3343124607131358 1103 98-99 1.4898297307096122 1103 100-101 1.4707723829916368 1104 1 -0.14256957187596697 1104 2 0.04341560097697794 1104 3 -0.0521294610571843 1104 4 0.14166995166477392 1104 5 0.10297082734116003 1104 6 0.06658585908726167 1104 7 0.08977905769835814 1104 8 -0.09686678813815064 1104 9 0.11624007154703264 1104 10-11 0.11937898226630494 1104 12-13 0.09992442308720939 1104 14-15 0.2483037888512385 1104 16-17 0.07259799122208221 1104 18-19 0.038331704815703915 1104 20-21 0.336644446313592 1104 22-23 0.08616374698235774 1104 24-25 0.28689471062330796 1104 26-27 -0.03936915453517997 1104 28-29 0.22478278716590694 1104 30-31 -0.014444223556687064 1104 32-33 0.172430067635176 1104 34-35 0.20919672600523143 1104 36-37 0.23802022059867767 1104 38-39 0.35824564597558606 1104 40-41 0.46388297228979525 1104 42-43 0.3106521594885905 1104 44-45 0.4896899930469658 1104 46-47 0.5979471805725325 1104 48-49 -0.053917565008788415 1104 50-51 0.16540831609709983 1104 52-53 0.4172713588297867 1104 54-55 0.7204205793098808 1104 56-57 0.36849066952939324 1104 58-59 0.5537529406005817 1104 60-61 0.43088109313815437 1104 62-63 0.2896625634632173 1104 64-65 0.24510433165659862 1104 66-67 0.22698477939099604 1104 68-69 0.560279328570715 1104 70-71 0.6596592252519642 1104 72-73 0.9124151284414701 1104 74-75 1.2429763411290722 1104 76-77 0.8860436523751325 1104 78-79 0.9319949775771192 1104 80-81 0.6743506520150646 1104 82-83 0.337076152662231 1104 84-85 0.6397560815309369 1104 86-87 0.463777531873383 1104 88-89 0.6007217785314616 1104 90-91 0.6825841382150664 1104 92-93 1.2394239075833724 1104 94-95 0.6873967513230355 1104 96-97 0.938645603787716 1104 98-99 1.6007743654128106 1104 100-101 0.9780735625959025 1105 1 -0.06477116703548802 1105 2 0.03719310372724749 1105 3 -0.04463496160723679 1105 4 0.09395267993760115 1105 5 -0.06950579532110623 1105 6 0.06747969847119606 1105 7 -0.15860515572298794 1105 8 -0.015321133572690826 1105 9 0.2277637239122683 1105 10-11 0.12202612198027651 1105 12-13 0.13918459910481573 1105 14-15 0.051263772349592784 1105 16-17 -0.03407830140717749 1105 18-19 0.09259806895212108 1105 20-21 0.10462436930588837 1105 22-23 -0.030430724964830347 1105 24-25 -0.007092500597813967 1105 26-27 0.1646324956298315 1105 28-29 0.15475390927812072 1105 30-31 0.36397643100877275 1105 32-33 0.26200308743715794 1105 34-35 0.014717903122942744 1105 36-37 -0.21462348127150932 1105 38-39 -0.006526768765887425 1105 40-41 0.16503121211377447 1105 42-43 0.5757958613587775 1105 44-45 0.5854511316608182 1105 46-47 0.2791387372281946 1105 48-49 -0.14892237799008257 1105 50-51 -0.39954974220872685 1105 52-53 -0.2295417599820908 1105 54-55 -0.47031099384743413 1105 56-57 -0.43766650868843016 1105 58-59 -0.6575025599494708 1105 60-61 -0.26163815878704355 1105 62-63 -0.334804570750201 1105 64-65 -0.27251461773834507 1105 66-67 -0.41243697528447143 1105 68-69 -0.211602547116847 1105 70-71 -0.10170284845541033 1105 72-73 -0.27864303987536587 1105 74-75 -0.38800550705574466 1105 76-77 -0.6791034873388391 1105 78-79 -0.3341285097716167 1105 80-81 -0.12613408395853298 1105 82-83 -0.11910852830586549 1105 84-85 -0.10574034311153113 1105 86-87 -0.7409116870485093 1105 88-89 -0.4864103721836095 1105 90-91 -0.2586459222909845 1105 92-93 -0.25940722552993734 1105 94-95 0.054936630310933765 1105 96-97 0.09212813954129118 1105 98-99 0.6577566261388803 1105 100-101 0.32049999273891316 1106 1 -0.03104179409819352 1106 2 0.10772115653252712 1106 3 0.04592609449836971 1106 4 0.1311143961092185 1106 5 -0.031056950436621378 1106 6 0.10797474797615081 1106 7 -0.050915386746090974 1106 8 0.013688767417406211 1106 9 0.2623511826581435 1106 10-11 0.06118453782185895 1106 12-13 0.22464664530942713 1106 14-15 -0.01190454448209266 1106 16-17 -0.060596453222359514 1106 18-19 0.10451226037125849 1106 20-21 0.0865055574247009 1106 22-23 0.16095541364902743 1106 24-25 0.2766863772899697 1106 26-27 0.22646417879815317 1106 28-29 0.1151994538325809 1106 30-31 0.12965299866554147 1106 32-33 0.003471734301847107 1106 34-35 0.18739117044967202 1106 36-37 0.29295077615422827 1106 38-39 0.18817620153114234 1106 40-41 0.2970774167342398 1106 42-43 0.22988827059970163 1106 44-45 0.49739832638029213 1106 46-47 0.4346832916836405 1106 48-49 0.588651879435659 1106 50-51 0.27825553831932837 1106 52-53 0.47997969216311986 1106 54-55 0.3043094681987739 1106 56-57 0.15578233619606152 1106 58-59 0.4530584961561317 1106 60-61 0.3686588709159295 1106 62-63 0.3987597856854421 1106 64-65 0.2342015538788189 1106 66-67 0.46899866827988745 1106 68-69 0.3199321063484959 1106 70-71 0.4268120030297453 1106 72-73 0.3863734617748058 1106 74-75 0.5188096744624069 1106 76-77 0.5134047634862391 1106 78-79 0.8529671997993375 1106 80-81 0.7062256520150605 1106 82-83 0.9304789304400067 1106 84-85 0.9207283037531546 1106 86-87 1.0690553096511621 1106 88-89 0.8039856674203527 1106 90-91 0.9497369159928404 1106 92-93 1.094701685361148 1106 94-95 1.5983689735452593 1106 96-97 1.3406594926766076 1106 98-99 1.261816032079473 1106 100-101 1.2050874514847862 1107 1 0.2093503627645532 1107 2 0.15105448986586367 1107 3 0.14762544090360308 1107 4 0.20457844839680206 1107 5 0.22940056590324787 1107 6 0.10621004209379237 1107 7 0.11490160671796446 1107 8 0.09349268898603214 1107 9 0.2886256924620625 1107 10-11 0.24049826331205537 1107 12-13 0.3184701747211989 1107 14-15 0.11155950780548807 1107 16-17 0.268063677496599 1107 18-19 0.3456233714823753 1107 20-21 0.48186503454888197 1107 22-23 0.39468090384510646 1107 24-25 0.22776480866252058 1107 26-27 0.28701973435370576 1107 28-29 0.45497069566264514 1107 30-31 0.28978371761978394 1107 32-33 0.18866781273322175 1107 34-35 0.21471143188757935 1107 36-37 0.330238357853581 1107 38-39 0.36794744336121 1107 40-41 -0.01671343293896399 1107 42-43 0.011326179096435851 1107 44-45 -0.042274876234088765 1107 46-47 -0.06453239459087001 1107 48-49 -0.390008251283291 1107 50-51 -0.18334576887022536 1107 52-53 -0.40554318365386877 1107 54-55 -0.14477549912148646 1107 56-57 -0.079936618052308 1107 58-59 0.2716859471365254 1107 60-61 0.06813599509893464 1107 62-63 0.3250996549664862 1107 64-65 0.2209009002840432 1107 66-67 0.20216860292040906 1107 68-69 0.33101053772104194 1107 70-71 0.16119108799706083 1107 72-73 0.288334246088521 1107 74-75 0.4811299359003165 1107 76-77 0.2922936523751325 1107 78-79 0.6017253697339768 1107 80-81 0.6313563709693106 1107 82-83 0.22443317880602365 1107 84-85 0.6248459508119772 1107 86-87 0.7051991005008347 1107 88-89 0.6226784778778693 1107 90-91 1.0599329944242157 1107 92-93 0.7627082213088592 1107 94-95 0.7639898885779388 1107 96-97 0.5184045907158215 1107 98-99 0.5197898882886243 1107 100-101 0.7016233991972065 1108 1 0.028958205901808753 1108 2 0.014387823199200511 1108 3 0.31925942783170314 1108 4 0.09444772944254964 1108 5 0.10227638289671148 1108 6 0.16464141464281568 1108 7 0.08575127992057929 1108 8 0.24035543408407278 1108 9 0.13235118265814094 1108 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-0.7096758209052965 2316 66-67 -0.987780260908373 2316 68-69 -0.8508191889882966 2316 70-71 -1.229526511650743 2316 72-73 -1.0639719613685479 2316 74-75 -1.1973302219106472 2316 76-77 -1.0352826285690249 2316 78-79 -1.0329049936376222 2316 80-81 -1.2951819472940933 2316 82-83 -1.3015158882128404 2316 84-85 -1.7059729052278456 2316 86-87 -1.7069550530431385 2316 88-89 -1.3606430027005452 2316 90-91 -1.8788813914337545 2316 92-93 -1.38618777923298 2316 94-95 -1.492960905556643 2316 96-97 -1.576637571226673 2316 98-99 -1.3375622062624934 2316 100-101 -0.6727881961836069 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 24.0 6 56.0 7 89.0 8 137.0 9 245.0 10 455.0 11 545.0 12 833.0 13 1057.0 14 1398.0 15 1833.0 16 2394.0 17 3276.0 18 3099.0 19 2316.0 20 2181.0 21 2362.0 22 2686.0 23 2843.0 24 3232.0 25 3734.0 26 4276.0 27 4801.0 28 5648.0 29 6920.0 30 8405.0 31 10098.0 32 12281.0 33 15723.0 34 20973.0 35 29633.0 36 43861.0 37 68702.0 38 50266.0 39 2719.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.69783538887491 22.707022401208153 17.147306821042033 19.447835388874907 2 14.547425949835166 24.698221291851034 37.85677576243779 22.897576995876005 3 20.358624666257192 27.084560712987454 33.06184739962646 19.4949672211289 4 11.796006367750103 20.48272058362686 40.34527495393409 27.375998094688946 5 10.979354951928501 39.964526116549884 36.22159268962232 12.834526241899294 6 25.74050164834476 39.80501899043584 17.661418704639182 16.793060656580217 7 22.306554520726525 35.64529877032228 24.10343833435702 17.944708374594182 8 22.8057585519636 32.61090289181092 22.15394161224413 22.42939694398135 9 21.90449628338995 20.517818418842523 25.39955124910688 32.17813404866064 10-11 22.521998821715535 27.541302631084147 28.8523007884478 21.084397758752523 12-13 25.942000827306117 26.14475350038232 28.308911089662438 19.604334582649134 14-15 21.790789865404804 28.99186788047821 29.27547359019758 19.941868663919397 16-17 18.275846399969918 31.816679019058196 30.081181066212476 19.82629351475941 18-19 18.142560779176826 32.01757409763527 30.886758631927947 18.95310649125996 20-21 20.270723391453675 29.096262080549533 29.5244870075335 21.10852752046329 22-23 19.081627536758717 29.03640773656568 31.01708512478534 20.86487960189027 24-25 21.282449828898052 29.553160685410585 30.039986462263556 19.124403023427806 26-27 20.134249219699914 28.000864910939242 33.174505183198164 18.690380686162676 28-29 18.045144590546148 31.07819296288404 31.50704463692543 19.369617809644385 30-31 19.34313774646828 29.137314012810712 30.513650550910665 21.005897689810347 32-33 19.606371510585756 29.567419180966947 31.514408914850144 19.311800393597153 34-35 21.04760874981783 28.881168522845336 30.49539363680954 19.5758290905273 36-37 19.89248660000627 28.26787449456164 32.66652038993198 19.17311851550011 38-39 19.516819792304908 29.405607172015223 31.852065433199563 19.225507602480306 40-41 19.131809620513398 30.022344863031947 31.544960810306844 19.300884706147812 42-43 18.88267158479621 30.046867434575976 29.66797924733188 21.402481733295936 44-45 19.76064229843562 28.59772989370237 30.197835188527876 21.443792619334133 46-47 21.258083167112968 29.69842167691148 30.223641471680907 18.819853684294642 48-49 20.10040723847189 29.010452819722936 30.626088019291725 20.26305192251344 50-51 17.90718772572793 29.84552179288106 32.345000806940625 19.90228967445038 52-53 19.324021961216893 30.951746744049036 29.4188801283578 20.305351166376273 54-55 18.696178096443838 28.688406432931796 30.472285245120773 22.14313022550359 56-57 20.643167830327037 28.888652117778307 29.192781127392607 21.27539892450205 58-59 21.580726183891585 28.65046622365887 29.720798542179494 20.048009050270053 60-61 19.85531340145125 29.845645691758314 30.950459647105095 19.34858125968534 62-63 18.905308152370626 29.864134990684843 30.887470679575753 20.343086177368786 64-65 18.78764423107055 31.094839959135832 29.727269308630987 20.390246501162622 66-67 19.542619868379816 29.43027264180508 28.66029457850204 22.36681291131307 68-69 18.71545976606263 29.247185465485227 30.19108280254777 21.846271965904375 70-71 20.895466261369602 29.039900347061725 28.864724270034394 21.19990912153428 72-73 19.47585143587751 27.554621006054376 31.4283878812189 21.541139676849216 74-75 18.457387467565837 30.938898429371875 28.933777905912734 21.669936197149557 76-77 19.260391917005812 28.396966916809774 29.426082319822466 22.916558846361948 78-79 19.215690575876852 28.213139619424876 30.526079925790356 22.045089878907913 80-81 20.2044765478718 28.405788018144356 29.259418847880415 22.13031658610343 82-83 19.019109517780812 27.980652318337366 31.02679970417539 21.973438459706433 84-85 18.909458411350407 28.376957608329484 30.06008946828263 22.65349451203748 86-87 18.59978953872597 28.92619270536833 29.877189103061724 22.59682865284397 88-89 18.870697686129365 28.175592315633104 28.939591358863964 24.014118639373567 90-91 19.1978659251092 26.765904504552683 29.909764241977165 24.126465328360954 92-93 19.839678102711307 28.216622585456964 29.95584566980458 21.98785364202715 94-95 18.929632989716566 27.341741210978814 30.13329447471134 23.59533132459328 96-97 17.15955099840806 27.62309312207779 31.054689948230692 24.162665931283453 98-99 18.448613008761924 28.15222432530679 28.88144452661795 24.517718139313335 100-101 18.24127405940346 26.10135816938572 30.526533502973933 25.130834268236885 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2750.0 1 1692.5 2 518.5 3 367.5 4 285.0 5 278.0 6 444.5 7 748.0 8 968.0 9 996.0 10 795.0 11 523.5 12 438.0 13 438.5 14 441.0 15 429.0 16 416.0 17 423.0 18 445.0 19 475.5 20 541.0 21 629.0 22 740.5 23 878.0 24 1057.0 25 1305.0 26 1787.5 27 2420.0 28 2796.0 29 3266.0 30 4240.0 31 5158.5 32 6203.5 33 7599.0 34 9104.0 35 10499.0 36 11901.0 37 12963.5 38 12842.5 39 12238.5 40 11735.0 41 11070.0 42 10303.5 43 10158.0 44 10302.5 45 10351.5 46 11007.0 47 16545.5 48 27864.5 49 26316.5 50 14839.5 51 9625.5 52 6911.0 53 4838.0 54 3440.0 55 2720.5 56 2218.0 57 1795.0 58 1534.0 59 1288.5 60 1005.5 61 821.5 62 603.0 63 383.5 64 232.5 65 154.0 66 98.5 67 76.5 68 63.0 69 38.0 70 25.5 71 17.5 72 11.5 73 13.0 74 12.0 75 7.5 76 5.5 77 4.0 78 3.0 79 1.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39735763440590643 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.401205861338481E-4 16-17 0.0020369279366233375 18-19 6.267470574225654E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012064880855384383 36-37 0.024443135239480048 38-39 0.010811386740539254 40-41 0.005954097045514371 42-43 0.005170663223736165 44-45 0.0012534941148451308 46-47 0.0010968073504894894 48-49 0.00235030146533462 50-51 4.7006029306692404E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.7006029306692404E-4 60-61 0.0014101808792007721 62-63 0.0014101808792007721 64-65 6.267470574225654E-4 66-67 0.0025069882296902615 68-69 0.001723554407912055 70-71 1.5668676435564135E-4 72-73 0.0 74-75 0.0 76-77 0.09103501009062763 78-79 0.0037604823445353923 80-81 1.5668676435564135E-4 82-83 0.0 84-85 1.5668676435564135E-4 86-87 0.08915476891835993 88-89 0.42994848139187986 90-91 0.38184564473469795 92-93 0.0 94-95 7.834338217782067E-4 96-97 0.0 98-99 0.0 100-101 6.267470574225654E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 319108.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.265196748480115 #Duplication Level Percentage of deduplicated Percentage of total 1 77.65313498537365 35.14984433249223 2 12.108752034294628 10.962100864218103 3 3.8638531006934524 5.246942124303422 4 1.8385025783196247 3.3288072372090314 5 0.9878523482796154 2.2357665451662445 6 0.6650816026999402 1.8063029760004365 7 0.452800423999927 1.434727019610634 8 0.3667199245731972 1.3279719629914848 9 0.27947268217820875 1.1385347350159865 >10 1.616446417821837 13.639324972118914 >50 0.09960033824085932 3.0394324879252674 >100 0.044958486011499 4.18031546958984 >500 0.008300028186738277 2.757968766598031 >1k 0.01383338031123046 11.808013626092034 >5k 6.916690155615231E-4 1.9439468806683136 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6209 1.9457362397683542 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4392 1.3763365380999535 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2720 0.852375998094689 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2644 0.8285596099126314 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2327 0.7292202013111548 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2219 0.6953758602103363 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2140 0.670619351442145 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2030 0.6361482632839038 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2029 0.6358348897551925 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1987 0.6226732015493187 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1759 0.5512240370031463 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1667 0.5223936723617082 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1571 0.4923098136054251 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1563 0.4898028253757349 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1556 0.4876092106747559 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1385 0.43402233726512657 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1306 0.40926582849693516 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1145 0.35881269037441865 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1124 0.35223184627148174 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1111 0.3481579903982351 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1040 0.325908469859734 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 939 0.29425774345989447 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 932 0.29206412875891546 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 917 0.28736352582824626 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 859 0.26918786116299187 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 839 0.2629203905887662 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 720 0.22562894067212355 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 702 0.21998821715532046 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 688 0.2156009877533625 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 570 0.17862291136543113 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 564 0.17674267019316345 RNA PCR Primer, Index 26 (100% over 22bp) CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 553 0.17329556137733934 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 526 0.1648344761021347 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 436 0.13663085851811926 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 430 0.13475061734585156 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 401 0.12566278501322437 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 396 0.12409591736966795 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 388 0.12158892913997768 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 386 0.12096218208255513 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 356 0.11156097622121665 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 352 0.11030748210637152 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 335 0.10498013211827971 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 328 0.10278651741730072 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 325 0.10184639683116688 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 324 0.10153302330245559 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 323 0.10121964977374431 RNA PCR Primer, Index 26 (100% over 24bp) GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 320 0.10027952918761046 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.267470574225654E-4 0.0 18-19 0.0 0.0 0.0 0.0010968073504894894 0.0 20-21 0.0 0.0 0.0 0.0018802411722676961 0.0 22-23 0.0 0.0 0.0 0.004387229401957958 0.0 24-25 0.0 0.0 0.0 0.007834338217782068 0.0 26-27 0.0 0.0 0.0 0.01864572495832132 0.0 28-29 0.0 0.0 0.0 0.05625054840367524 0.0 30-31 0.0 0.0 0.0 0.13443724381714028 0.0 32-33 0.0 0.0 0.0 0.22750918184439123 0.0 34-35 0.0 0.0 0.0 0.35191847274277044 0.0 36-37 0.0 0.0 0.0 0.5200433708963736 0.0 38-39 0.0 0.0 0.0 0.7267132130814646 0.0 40-41 0.0 0.0 0.0 0.943254321420961 0.0 42-43 0.0 0.0 0.0 1.1482006091981398 0.0 44-45 0.0 0.0 0.0 1.4057936497988142 0.0 46-47 0.0 0.0 0.0 1.6980144653220854 0.0 48-49 0.0 0.0 0.0 1.9670456397207214 0.0 50-51 0.0 0.0 0.0 2.227615728844153 0.0 52-53 0.0 0.0 0.0 2.4773744312270454 0.0 54-55 0.0 0.0 0.0 2.7703786805720947 0.0 56-57 0.0 0.0 0.0 3.138905950336563 0.0 58-59 0.0 0.0 0.0 3.4746856863507025 0.0 60-61 0.0 0.0 0.0 3.846973438459706 0.0 62-63 0.0 0.0 0.0 4.215657394988531 0.0 64-65 0.0 0.0 0.0 4.610351354400391 0.0 66-67 0.0 0.0 0.0 5.087462551863319 0.0 68-69 0.0 0.0 0.0 5.62771851536157 0.0 70-71 0.0 0.0 0.0 6.188343758226055 0.0 72-73 0.0 0.0 0.0 6.756019905486544 0.0 74-75 0.0 0.0 0.0 7.40141268786743 0.0 76-77 0.0 0.0 0.0 8.027376311468219 0.0 78-79 0.0 0.0 0.0 8.8282023640899 0.0 80-81 0.0 0.0 0.0 9.6378028755155 0.0 82-83 0.0 0.0 0.0 10.413245672311568 0.0 84-85 0.0 0.0 0.0 11.278469985083419 0.0 86-87 0.0 0.0 0.0 12.146671346378028 0.0 88-89 0.0 0.0 0.0 13.089142233977212 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGGCG 35 2.7989235E-4 54.23047 9 GTAAGCG 40 2.2786298E-7 41.520203 94-95 GGATATG 105 1.033186E-9 40.67285 7 CTCCGAC 65 2.0736479E-10 36.501278 94-95 GTCTTGA 60 0.0038939263 31.753853 1 CTGGTCG 90 2.4243565E-5 31.63444 9 CATATAG 140 1.6529157E-8 30.619787 1 CTAGCCA 80 4.5132803E-4 29.657286 4 ATATGAG 150 3.3536708E-8 28.470995 9 GTGTAGG 85 6.2948844E-4 28.018106 1 GTGTAGA 85 6.2948844E-4 28.018106 1 CTCCCGC 85 6.4351596E-4 27.912743 7 GTACCCA 70 0.008270926 27.217588 1 GTCGCTC 35 0.008430587 27.115234 26-27 ACTCCCG 90 8.9865935E-4 26.362034 6 CCCGCTG 90 8.9865935E-4 26.362034 9 GATATGA 170 1.128592E-7 25.121468 8 GGTACTC 100 0.0016608513 23.72583 3 CGCAGAA 140 1.788296E-5 23.72583 9 GAGTACT 1255 0.0 23.449604 12-13 >>END_MODULE