##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938922_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 321985 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.707054676460082 33.0 31.0 34.0 30.0 34.0 2 31.766576082736773 33.0 31.0 34.0 30.0 34.0 3 31.787955960681398 34.0 31.0 34.0 30.0 34.0 4 35.29815674643229 37.0 35.0 37.0 33.0 37.0 5 35.328291690606704 37.0 35.0 37.0 33.0 37.0 6 35.44176592077271 37.0 35.0 37.0 33.0 37.0 7 35.384539652468284 37.0 35.0 37.0 33.0 37.0 8 35.37417270990885 37.0 35.0 37.0 33.0 37.0 9 36.960541640138516 39.0 37.0 39.0 33.0 39.0 10-11 36.861075826513655 39.0 37.0 39.0 33.0 39.0 12-13 36.7896718791248 39.0 37.0 39.0 33.0 39.0 14-15 37.93547991366057 40.0 38.0 41.0 33.0 41.0 16-17 37.86519247791046 40.0 38.0 41.0 33.0 41.0 18-19 37.82617047377983 40.0 38.0 41.0 33.0 41.0 20-21 37.70636830908272 40.0 38.0 41.0 32.5 41.0 22-23 37.78551795891113 40.0 38.0 41.0 33.0 41.0 24-25 37.70530614780192 40.0 38.0 41.0 32.5 41.0 26-27 37.589488640775194 40.0 37.0 41.0 32.5 41.0 28-29 37.48660651893722 40.0 37.0 41.0 32.0 41.0 30-31 37.35245585974502 40.0 37.0 41.0 32.0 41.0 32-33 37.12602139851235 40.0 36.0 41.0 31.5 41.0 34-35 36.80423001071479 40.0 36.0 41.0 30.5 41.0 36-37 36.522344208581146 39.5 35.5 41.0 30.0 41.0 38-39 36.322431169153845 39.0 35.0 41.0 30.0 41.0 40-41 36.241223970060716 39.0 35.0 41.0 30.0 41.0 42-43 36.0351771045235 39.0 35.0 41.0 30.0 41.0 44-45 35.92620463686197 39.0 35.0 41.0 29.0 41.0 46-47 35.65909592061742 39.0 35.0 41.0 27.0 41.0 48-49 35.446443157289934 39.0 35.0 41.0 26.0 41.0 50-51 34.30531546500613 38.0 34.0 40.0 23.5 40.5 52-53 34.49411152693448 38.5 34.5 39.5 24.0 40.5 54-55 35.05555383014737 39.0 35.0 40.5 24.0 41.0 56-57 34.95282388931162 39.0 35.0 41.0 23.5 41.0 58-59 34.809641753497836 39.0 35.0 41.0 23.0 41.0 60-61 34.504475363759184 39.0 35.0 40.5 22.0 41.0 62-63 34.1958165753063 38.0 34.0 40.0 22.0 41.0 64-65 33.811253629827476 38.0 34.0 40.0 19.5 41.0 66-67 33.37381865614858 37.0 33.5 40.0 18.0 41.0 68-69 32.97995403512586 37.0 33.0 39.0 15.0 41.0 70-71 32.462647328291695 36.0 32.5 39.0 10.5 40.5 72-73 31.962622482413778 35.5 32.0 38.5 7.5 40.0 74-75 31.49311924468531 35.0 31.0 37.5 4.0 40.0 76-77 31.045485038122894 35.0 31.0 37.0 2.0 39.0 78-79 30.551604888426482 35.0 31.0 36.5 2.0 39.0 80-81 30.05472149323726 35.0 30.5 36.0 2.0 38.5 82-83 29.603886827026102 34.0 30.0 35.5 2.0 37.0 84-85 29.179009891765144 34.0 29.0 35.0 2.0 37.0 86-87 28.81510629377145 34.0 29.0 35.0 2.0 36.5 88-89 28.31587185738466 34.0 29.0 35.0 2.0 36.0 90-91 27.835296675310964 34.0 28.0 35.0 2.0 36.0 92-93 27.40378589064708 34.0 27.0 35.0 2.0 35.0 94-95 26.881586098731308 34.0 25.5 35.0 2.0 35.0 96-97 26.297515412208643 34.0 25.0 35.0 2.0 35.0 98-99 25.669483050452662 33.0 22.0 35.0 2.0 35.0 100-101 23.16701243846763 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.030678939265374794 1101 2 0.15701079815341856 1101 3 0.08711210992595753 1101 4 0.07954809692248688 1101 5 0.22342886089096226 1101 6 -0.09941844130932509 1101 7 -0.32727904955438447 1101 8 -0.04557347540543333 1101 9 -0.31656060604468195 1101 10-11 0.05637779460547421 1101 12-13 0.2650448794205502 1101 14-15 0.03027249173803881 1101 16-17 0.18793666131171705 1101 18-19 0.08625817302142025 1101 20-21 0.05806111387791901 1101 22-23 0.1455724153958755 1101 24-25 0.2019470893554498 1101 26-27 0.27896481168978227 1101 28-29 0.2559855412967167 1101 30-31 0.5229692998219448 1101 32-33 0.16592992742228319 1101 34-35 0.4411739874354197 1101 36-37 0.37281879007257857 1101 38-39 0.21582819971103362 1101 40-41 0.3857274752608859 1101 42-43 0.16998992543596358 1101 44-45 0.37928230740723023 1101 46-47 0.19379971643087401 1101 48-49 0.12160278344145325 1101 50-51 -0.08272578505175687 1101 52-53 -0.09731742915011665 1101 54-55 0.2646607257941369 1101 56-57 0.2006685499256946 1101 58-59 -0.268046556233692 1101 60-61 -0.18760073735991512 1101 62-63 -0.2227646188836232 1101 64-65 0.010851753287106192 1101 66-67 -0.05285182058063498 1101 68-69 0.20756769530341757 1101 70-71 0.19377224556242112 1101 72-73 -0.2159756530440724 1101 74-75 -0.1147686283253968 1101 76-77 0.2808699378072994 1101 78-79 0.5326674752131382 1101 80-81 0.3312130600287517 1101 82-83 0.16976195812484107 1101 84-85 -0.587085574048352 1101 86-87 -0.07900420605939473 1101 88-89 -0.151774855867167 1101 90-91 0.3142366497435525 1101 92-93 0.09448883070837155 1101 94-95 -0.31918286956457464 1101 96-97 -0.10296900876913995 1101 98-99 0.05022745623157476 1101 100-101 -0.2028398520383874 1102 1 -0.1624119865413718 1102 2 -0.2682673116194998 1102 3 -0.320422136649384 1102 4 -0.07267678126083865 1102 5 0.04337002113735622 1102 6 -0.30806972383207665 1102 7 -0.044388546658360895 1102 8 -0.20053348276039884 1102 9 0.06548407539321488 1102 10-11 -0.029870711416407403 1102 12-13 -0.2601343979537276 1102 14-15 0.05208457227494989 1102 16-17 -0.2299290185632472 1102 18-19 -0.052348520000556675 1102 20-21 -0.14455679547209144 1102 22-23 -0.19622679394493048 1102 24-25 -0.18782030974356445 1102 26-27 -0.20294656093134478 1102 28-29 -0.11907191940017725 1102 30-31 -0.15723893774356412 1102 32-33 -0.1178109937876144 1102 34-35 -0.017948472801776916 1102 36-37 -0.2702008844700359 1102 38-39 -0.10804327220218823 1102 40-41 0.05793718382022206 1102 42-43 -0.33220507318497283 1102 44-45 -0.24421222233443274 1102 46-47 -0.09046984319034834 1102 48-49 0.30340842838031534 1102 50-51 0.4606225646770312 1102 52-53 0.08800481043801511 1102 54-55 0.3825011229624735 1102 56-57 0.06548651443797127 1102 58-59 0.021796690985205203 1102 60-61 0.1288123360978375 1102 62-63 0.1421797591617988 1102 64-65 0.12201521747760324 1102 66-67 0.07605550680742823 1102 68-69 0.5295774406376097 1102 70-71 0.1886007828429186 1102 72-73 0.2509384776905037 1102 74-75 0.05209723542379763 1102 76-77 0.025709967227175667 1102 78-79 -0.272931495091175 1102 80-81 -0.5084945799455056 1102 82-83 -0.48040582711005797 1102 84-85 -0.5600560622344375 1102 86-87 -0.543504987524873 1102 88-89 -0.3066544182465023 1102 90-91 -0.3115333235946842 1102 92-93 -0.5167091099002512 1102 94-95 -0.37272474692046487 1102 96-97 -0.5200762074452463 1102 98-99 -0.7671049883763139 1102 100-101 -0.5038074901739051 1103 1 -0.014760979829965493 1103 2 0.05388033938721293 1103 3 0.28024900429021216 1103 4 0.49108160800090417 1103 5 0.23464518892259179 1103 6 0.3832054439531589 1103 7 0.2744033996503603 1103 8 0.04778866489061073 1103 9 0.1292424646549577 1103 10-11 0.12281385234198439 1103 12-13 0.36906023291875556 1103 14-15 0.13429933737562294 1103 16-17 0.2650374243897815 1103 18-19 0.24630919812024388 1103 20-21 0.2430270971453652 1103 22-23 0.4262564275315839 1103 24-25 0.26687767683362296 1103 26-27 0.5520869961156336 1103 28-29 0.5134784161702939 1103 30-31 0.5877275052094646 1103 32-33 0.44594739547413553 1103 34-35 0.15487031914453553 1103 36-37 0.16268502157023335 1103 38-39 0.24430572108640547 1103 40-41 0.5948499354980825 1103 42-43 0.2147747925868373 1103 44-45 0.24404281122262006 1103 46-47 0.2132214319774448 1103 48-49 0.09871044180314215 1103 50-51 0.037803772730718777 1103 52-53 0.2641793070823084 1103 54-55 0.18619239813025246 1103 56-57 0.34401000437085827 1103 58-59 0.1342127983677628 1103 60-61 0.3351881750240153 1103 62-63 0.403924725604746 1103 64-65 -0.04744786977072124 1103 66-67 0.14149174841816858 1103 68-69 0.08159086345640532 1103 70-71 0.12987595062815416 1103 72-73 0.047918343462320934 1103 74-75 0.11753347703453798 1103 76-77 0.3461797658848944 1103 78-79 0.33109535054640915 1103 80-81 0.09888797039006292 1103 82-83 0.38368813262148294 1103 84-85 0.09766205857093269 1103 86-87 -0.2414915647060809 1103 88-89 -0.08853361287737371 1103 90-91 0.17504385761337105 1103 92-93 -0.023420519296227127 1103 94-95 0.37224169603255675 1103 96-97 0.12086338987018763 1103 98-99 0.1406131324290527 1103 100-101 0.6018972078126765 1104 1 -0.026803287195523495 1104 2 0.20469590306064944 1104 3 0.13210950892023732 1104 4 -0.029477392933593194 1104 5 -0.012742458685934821 1104 6 0.07237798664282025 1104 7 -0.24751487407319672 1104 8 -0.18706818669379288 1104 9 0.016975027733707293 1104 10-11 0.07438975923179925 1104 12-13 0.17006482046441818 1104 14-15 0.060335782877281474 1104 16-17 0.2082028308972994 1104 18-19 0.07448862212839913 1104 20-21 0.3111606456968872 1104 22-23 0.21543109408497685 1104 24-25 -9.096819294427405E-4 1104 26-27 0.20795735547337557 1104 28-29 0.2889966389211267 1104 30-31 0.28465215052421655 1104 32-33 0.530742655010279 1104 34-35 0.49410445388245705 1104 36-37 0.36497242281057396 1104 38-39 0.6555282170510637 1104 40-41 0.5717210594467659 1104 42-43 0.7787986648151914 1104 44-45 0.64287256230579 1104 46-47 0.7512385927963976 1104 48-49 0.8548696466285506 1104 50-51 1.0649679898745532 1104 52-53 1.187189246764575 1104 54-55 1.1253118442265944 1104 56-57 0.8252350489719973 1104 58-59 0.662311515554066 1104 60-61 0.5929827549237672 1104 62-63 0.9402534147560502 1104 64-65 0.8478987447904913 1104 66-67 0.9049251865287786 1104 68-69 0.7766677473235362 1104 70-71 0.7581254621394962 1104 72-73 0.6877004350433182 1104 74-75 0.9323962754373873 1104 76-77 1.3452665044797492 1104 78-79 1.2105979416601613 1104 80-81 1.2839614560752217 1104 82-83 1.142863223947625 1104 84-85 1.464240938869974 1104 86-87 1.6735751246151338 1104 88-89 1.436464263258891 1104 90-91 1.5312689022145065 1104 92-93 1.8569953332226738 1104 94-95 1.526317390535997 1104 96-97 1.4929430772374488 1104 98-99 1.899087348723338 1104 100-101 2.1855986775263787 1105 1 -0.04405117050510654 1105 2 -0.03356799164972202 1105 3 0.14977653884730557 1105 4 0.03339459968942293 1105 5 -0.16099461729101705 1105 6 0.11372680045788996 1105 7 0.09601769361898249 1105 8 0.0980353425207241 1105 9 0.12742016144148494 1105 10-11 -0.19860043273735783 1105 12-13 0.2539884519507112 1105 14-15 -0.007806978460642711 1105 16-17 -0.1444252183943462 1105 18-19 -0.12624069298602336 1105 20-21 -0.14767915646590524 1105 22-23 -0.3006536573610816 1105 24-25 -0.05937015640341059 1105 26-27 -0.2834501388681474 1105 28-29 -0.17760785433060988 1105 30-31 -0.14591621228908735 1105 32-33 -0.3354762233533677 1105 34-35 -0.4165439659347996 1105 36-37 -0.34693762832531405 1105 38-39 -0.3118412187775874 1105 40-41 -0.2847002667331324 1105 42-43 -0.06761553587219993 1105 44-45 -0.07712701854759274 1105 46-47 -0.17216236285788966 1105 48-49 -0.02862855104098827 1105 50-51 0.3423173066655565 1105 52-53 0.3873736712762721 1105 54-55 0.34753290215265764 1105 56-57 0.19192546728032056 1105 58-59 -0.14567741897523234 1105 60-61 0.048553213470171386 1105 62-63 -0.1860459397581522 1105 64-65 0.2609991723200338 1105 66-67 0.2336513999582408 1105 68-69 0.1676057974534686 1105 70-71 0.05447259936810411 1105 72-73 0.19925840302052222 1105 74-75 0.21429333302813447 1105 76-77 -0.003002419990032479 1105 78-79 0.16418793243893504 1105 80-81 -0.08122003441015124 1105 82-83 -0.25656254518641575 1105 84-85 -0.09230193982899593 1105 86-87 -0.1922560431273439 1105 88-89 -0.20063859532103834 1105 90-91 -0.18233869272220105 1105 92-93 0.0363376921798384 1105 94-95 0.1788553233048802 1105 96-97 0.12220789407037458 1105 98-99 0.2507513607947587 1105 100-101 1.099652663610712 1106 1 0.31970118382990975 1106 2 0.3024487772912856 1106 3 0.40281945356468185 1106 4 0.3646115630529323 1106 5 0.23157682285317804 1106 6 0.424418012965738 1106 7 0.3238666921405624 1106 8 0.3467645079216197 1106 9 0.5298848717316957 1106 10-11 0.39502615454012613 1106 12-13 0.4257890878743211 1106 14-15 0.5333305957135721 1106 16-17 0.6281308299682493 1106 18-19 0.6875997590463001 1106 20-21 0.5734511268643914 1106 22-23 0.5319303392365242 1106 24-25 0.6363130564284702 1106 26-27 0.5489262712013243 1106 28-29 0.6113120881527294 1106 30-31 0.6934219411021658 1106 32-33 0.7087852749150514 1106 34-35 0.4163792574283036 1106 36-37 0.46063978626042257 1106 38-39 0.5384686420888087 1106 40-41 0.532846364289405 1106 42-43 0.5716750245101565 1106 44-45 0.5201854953758911 1106 46-47 0.39770822466262246 1106 48-49 0.38522810286669795 1106 50-51 0.450211670438172 1106 52-53 0.3608431003626862 1106 54-55 0.1992047331671003 1106 56-57 0.18540421244507144 1106 58-59 0.4807123981460961 1106 60-61 0.5359916969746337 1106 62-63 0.37528322159226946 1106 64-65 0.4685148172559437 1106 66-67 0.7310439106413469 1106 68-69 0.6464370346691766 1106 70-71 0.8161278234602847 1106 72-73 0.5734812193115104 1106 74-75 0.6736671305452013 1106 76-77 0.7111357415021615 1106 78-79 0.655808525022529 1106 80-81 0.7488222929892459 1106 82-83 0.7733552302960938 1106 84-85 0.4082853781530602 1106 86-87 0.4601175316139319 1106 88-89 0.5633659006993739 1106 90-91 0.6716368424459951 1106 92-93 1.0036149670244008 1106 94-95 1.0383901269584044 1106 96-97 0.6677098484215911 1106 98-99 0.8956518205229749 1106 100-101 1.1457368728579311 1107 1 -0.06802341094688913 1107 2 -0.006136385530769672 1107 3 0.34251716047521796 1107 4 0.1425814900687925 1107 5 0.05792927666408332 1107 6 0.18293527220111372 1107 7 0.1569215719170174 1107 8 0.12815404002193986 1107 9 0.2791985081397428 1107 10-11 0.3839906004175475 1107 12-13 0.2610129743043501 1107 14-15 0.0654162617060905 1107 16-17 0.12456955553028592 1107 18-19 0.1890799595328616 1107 20-21 0.21595095588413216 1107 22-23 0.4011744111068509 1107 24-25 0.2298148297379683 1107 26-27 0.30090231442513016 1107 28-29 0.359228571626268 1107 30-31 0.3809335433336969 1107 32-33 0.5838475605790876 1107 34-35 0.19559978287504975 1107 36-37 0.46333150397691014 1107 38-39 0.33628204889480173 1107 40-41 0.5338575903643843 1107 42-43 0.4917490833859901 1107 44-45 0.4525896731562753 1107 46-47 0.43184076855568776 1107 48-49 0.36640561945178973 1107 50-51 0.3181123260253145 1107 52-53 0.42917169510040054 1107 54-55 0.33545157003239723 1107 56-57 0.48982234221419674 1107 58-59 0.18820426437114435 1107 60-61 0.006548897197397707 1107 62-63 0.22815227025813556 1107 64-65 0.3038022106375351 1107 66-67 0.2385230754760883 1107 68-69 0.027395696528856206 1107 70-71 0.22765775477507333 1107 72-73 0.3319203161449522 1107 74-75 0.1768962147749562 1107 76-77 0.1390748920722551 1107 78-79 0.12135844637160886 1107 80-81 0.22876875174392453 1107 82-83 0.08389933831653451 1107 84-85 -0.022575092189192958 1107 86-87 0.04807465953647494 1107 88-89 0.25305139473460514 1107 90-91 0.3536037992905783 1107 92-93 0.2598899423543948 1107 94-95 0.023167999182415855 1107 96-97 -0.09591727783999815 1107 98-99 -0.043151903915479295 1107 100-101 0.621731245164991 1108 1 -0.09833866059276275 1108 2 0.0817902457749966 1108 3 0.0015843358425264853 1108 4 0.044935781455002655 1108 5 0.21254532141319515 1108 6 0.21717951054597506 1108 7 0.0999719146695881 1108 8 0.17070085185439865 1108 9 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2316 90-91 -2.777516357696822 2316 92-93 -3.005330375188297 2316 94-95 -2.854063962673539 2316 96-97 -3.0409514161121614 2316 98-99 -3.898011539665788 2316 100-101 -4.953352681466104 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2615.0 3 498.0 4 349.0 5 336.0 6 416.0 7 450.0 8 535.0 9 589.0 10 701.0 11 943.0 12 1247.0 13 2025.0 14 1954.0 15 1988.0 16 2514.0 17 3354.0 18 2832.0 19 1934.0 20 1964.0 21 2091.0 22 2164.0 23 2504.0 24 2810.0 25 3227.0 26 3533.0 27 4230.0 28 5104.0 29 5857.0 30 7201.0 31 8597.0 32 10662.0 33 13470.0 34 17981.0 35 26690.0 36 36946.0 37 60407.0 38 68824.0 39 12442.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.56561598873962 22.109078679396873 16.833267938441608 19.492037393421896 2 14.376614454947575 24.307513451848443 38.246767981647665 23.06910411155631 3 20.574018408626753 26.960462795736362 32.672359394088225 19.793159401548664 4 11.715988472407457 19.729033180689107 41.186272589635735 27.368705757267698 5 11.226279006143209 39.430633969209346 36.2467978212749 13.096289203372546 6 25.835519526538935 39.221238607964786 18.172549702221836 16.770692163274443 7 22.39851285861277 35.46633259867388 24.574824909618236 17.56032963309511 8 22.580524833541602 32.19035007553575 22.397125289864533 22.831999801058124 9 22.215072415111585 20.527096214643425 25.99825296017557 31.259578410069416 10-11 22.5599037623153 27.24884870696154 29.1016417291077 21.089605801615463 12-13 25.82683260049519 26.24104546394172 28.562036341933105 19.37008559362998 14-15 21.964401460500728 28.821729067477435 29.646952393831576 19.56691707819026 16-17 18.27856214757757 31.428931122591713 30.43282292307788 19.859683806752834 18-19 18.008110683314964 31.881140718334837 31.309600232533196 18.801148365816996 20-21 20.246248449483474 28.99240518921742 29.793855135901413 20.967491225397694 22-23 19.002226118966785 28.827152433185336 31.28008058799388 20.890540859853996 24-25 21.179047787847267 29.269881544845518 30.248586358456276 19.302484308850943 26-27 20.155136385686035 27.70641317462094 33.23755861537122 18.900891824321803 28-29 18.176362675289948 30.911818662355028 31.62588227977986 19.28593638257517 30-31 19.418746550799437 29.0528786736415 30.773009568373844 20.75536520718522 32-33 19.43923257842023 29.49663996567682 31.56364789514019 19.500479560762756 34-35 21.022098500269646 28.655417390138748 30.548706166943585 19.773777942648017 36-37 19.93662385366951 28.17692845898198 32.71446063120018 19.17198705614833 38-39 19.7284944935348 29.286462667992865 31.789316223968623 19.195726614503716 40-41 19.151440229013115 30.038772394804127 31.525671883145872 19.28411549303689 42-43 18.7868171123362 30.107557100189386 29.683667526936112 21.4219582605383 44-45 19.770652374214865 28.479022016497535 30.293844813208025 21.45648079607957 46-47 21.055885976431423 29.663709794528355 30.331543387323183 18.948860841717035 48-49 19.9172839911099 29.066024430585557 30.764857566343828 20.25183401196071 50-51 17.986166529498025 29.67449647135622 32.36950889461856 19.969828104527192 52-53 19.368532187178854 30.895880903221375 29.3432934657325 20.39229344386727 54-55 18.744388036347566 28.484492631638126 30.84889587787118 21.922223454143133 56-57 20.635074271530158 28.609877371966498 29.462575422875474 21.29247293362787 58-59 21.739951700097254 28.239832277260803 29.853367489825644 20.166848532816303 60-61 19.963927755657352 29.100409401250673 31.475997603504872 19.459665239587103 62-63 19.159191512284234 29.217197148901953 31.346776900797092 20.27683443801672 64-65 19.22855848679381 30.40048107711291 30.13151523671181 20.23944519938147 66-67 19.844970685962537 28.92693282844141 28.90662467565537 22.321471809940686 68-69 18.783763095159987 28.802322057357816 30.8061588259738 21.6077560215084 70-71 21.269409816602618 28.600774830505827 29.185178242259507 20.94463711063205 72-73 19.920555207570217 27.400487040626807 31.78088482691938 20.89807292488359 74-75 18.975626975895636 30.81256950779112 29.490671898234343 20.721131618078896 76-77 19.780897648124434 28.281694100009368 29.737483212043603 22.199925039822592 78-79 19.877216512068347 28.264090669922577 30.810942469440743 21.047750348568332 80-81 20.930573857001004 28.491759665765297 29.547640310503535 21.03002616673016 82-83 19.92667231926897 28.171664222931142 31.189872785218288 20.7117906725816 84-85 19.842681504223425 28.574998008923146 30.27045850986247 21.311861976990958 86-87 19.761250429405703 29.05718122482121 29.95518565941101 21.226382686362076 88-89 20.12228451843638 28.42076494198123 28.953475660227078 22.503474879355313 90-91 20.58744054917335 27.06240677251322 29.838262512983516 22.511890165329916 92-93 21.58248995206043 28.43227149821691 29.95480965539433 20.030428894328324 94-95 20.57258717052338 27.794439706208546 29.988190858224694 21.64478226504338 96-97 19.078397590794285 27.848943551422888 30.90887223118628 22.163786626596544 98-99 20.72495235714955 28.50401449592302 28.561029710393953 22.210003436533473 100-101 20.776669311161225 26.67384011844004 29.903298954904077 22.646191615494658 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3468.0 1 1982.0 2 465.5 3 387.5 4 303.0 5 308.5 6 458.5 7 779.5 8 1054.0 9 1068.5 10 854.5 11 634.0 12 560.5 13 550.5 14 558.5 15 545.5 16 595.5 17 632.0 18 574.5 19 570.0 20 654.0 21 737.5 22 843.0 23 1002.5 24 1160.0 25 1402.0 26 1893.5 27 2535.0 28 2940.5 29 3467.5 30 4384.0 31 5267.0 32 6308.5 33 7579.5 34 9022.0 35 10442.0 36 11820.5 37 12970.5 38 12918.5 39 12297.5 40 11735.5 41 11039.0 42 10273.5 43 9921.5 44 10074.5 45 10017.5 46 10906.0 47 16542.5 48 28123.0 49 26947.0 50 14453.0 51 8323.5 52 5836.5 53 4574.0 54 3659.5 55 3019.5 56 2437.5 57 1915.0 58 1636.0 59 1380.5 60 1137.5 61 932.5 62 696.0 63 500.0 64 340.5 65 243.5 66 192.5 67 171.5 68 148.5 69 108.0 70 78.5 71 71.5 72 57.0 73 50.0 74 50.5 75 36.0 76 21.0 77 19.0 78 17.0 79 10.5 80 8.0 81 6.0 82 4.5 83 2.5 84 0.5 85 1.0 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26678261409693 2 0.08758171964532509 3 0.08944516048884264 4 0.10000465860210879 5 0.10217867291954594 6 0.08354426448437038 7 0.0913086013323602 8 0.08789229311924468 9 0.09255089522803857 10-11 0.0874264329083653 12-13 0.09022159417364163 14-15 0.09767535754771185 16-17 0.08789229311924468 18-19 0.09643306365203348 20-21 0.09876236470643042 22-23 0.10839014239793779 24-25 0.1200366476699225 26-27 0.12329766914607823 28-29 0.11646505271984718 30-31 0.10947714955665637 32-33 0.10450797397394287 34-35 0.3708247278599935 36-37 0.5692811776946132 38-39 0.5498703355746386 40-41 0.5136885258630061 42-43 0.5393108374613724 44-45 0.5640014286379801 46-47 0.5498703355746386 48-49 0.5756479339099648 50-51 0.5650884357966987 52-53 0.5720763389598894 54-55 0.5585663928443871 56-57 0.5303042067177043 58-59 0.5262667515567495 60-61 0.4711399599360218 62-63 0.5458328804136838 64-65 0.580772396229638 66-67 0.595214062766899 68-69 0.597543363821296 70-71 0.5947482025560197 72-73 0.5865180054971505 74-75 0.5829464105470752 76-77 0.5636908551640605 78-79 0.5424165722005684 80-81 0.5376026833548148 82-83 0.5523549233659953 84-85 0.5610509806357439 86-87 0.5512679162072767 88-89 0.5683494572728544 90-91 0.5799959625448391 92-93 0.588381446340668 94-95 0.5882261596037083 96-97 0.5837228442318741 98-99 0.5885367330776279 100-101 0.5661754429554171 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 321985.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.27744151286865 #Duplication Level Percentage of deduplicated Percentage of total 1 79.15744487137599 37.42361470214603 2 11.274201399295954 10.660307945190327 3 3.695018369919776 5.240730446185724 4 1.6309883618690248 3.0843582754572907 5 0.9657555090701965 2.2829224797898453 6 0.624900500530714 1.7726218119121901 7 0.42144826412232117 1.394749696042014 8 0.30702296309458693 1.1612208144649572 9 0.24235248241555418 1.03120247816099 >10 1.5294408404272648 13.319744938482064 >50 0.09283099017776444 2.9843919619234405 >100 0.0381857973780875 3.7364039539975997 >500 0.006583758168635776 2.226465968255984 >1k 0.013167516337271552 11.80528096994893 >5k 6.583758168635775E-4 1.8759835580426134 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6027 1.8718263273133842 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4410 1.369629019985403 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2876 0.893209310992748 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2624 0.8149447955650109 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2623 0.8146342220910912 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2060 0.6397813562743606 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2037 0.63263816637421 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2021 0.6276689907914965 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1910 0.5931953351864218 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1889 0.5866732922341104 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1817 0.5643120021118996 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1726 0.5360498159852167 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1627 0.505303042067177 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1613 0.5009550134323028 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1520 0.47207168035778063 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1376 0.4273491001133593 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1300 0.4037455160954703 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1245 0.3866639750298927 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1113 0.3456682764725065 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1094 0.33976738046803423 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1046 0.3248598537198938 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 873 0.27113064273180426 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 865 0.2686460549404475 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 828 0.25715483640542264 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 805 0.250011646505272 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 740 0.22982437070049846 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 716 0.22237060732642824 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 673 0.20901594794788575 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 618 0.1919344068823082 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 521 0.1618087799121077 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 514 0.15963476559467055 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 473 0.14690125316396727 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 417 0.12950913862447008 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 413 0.1282668447287917 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 398 0.12360824261999782 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 368 0.11429103840241005 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 367 0.11398046492849045 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 366 0.11366989145457088 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 357 0.11087473018929453 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 356 0.11056415671537494 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.105734739195925E-4 0.0 10-11 0.0 0.0 0.0 3.105734739195925E-4 0.0 12-13 0.0 0.0 0.0 3.105734739195925E-4 0.0 14-15 0.0 0.0 0.0 3.105734739195925E-4 0.0 16-17 0.0 0.0 0.0 3.105734739195925E-4 0.0 18-19 0.0 0.0 0.0 3.105734739195925E-4 0.0 20-21 0.0 0.0 0.0 6.21146947839185E-4 0.0 22-23 0.0 0.0 0.0 0.0021740143174371475 0.0 24-25 0.0 0.0 0.0 0.005279749056633073 0.0 26-27 0.0 0.0 0.0 0.01335465937854248 0.0 28-29 0.0 0.0 0.0 0.047828314983617254 0.0 30-31 0.0 0.0 0.0 0.12717983757007315 0.0 32-33 0.0 0.0 0.0 0.23029023091137785 0.0 34-35 0.0 0.0 0.0 0.3568489215336118 0.0 36-37 0.0 0.0 0.0 0.526732611767629 0.0 38-39 0.0 0.0 0.0 0.7334192586611177 0.0 40-41 0.0 0.0 0.0 0.9584297405158626 0.0 42-43 0.0 0.0 0.0 1.1846825162662857 0.0 44-45 0.0 0.0 0.0 1.451154556889296 0.0 46-47 0.0 0.0 0.0 1.7444912030063513 0.0 48-49 0.0 0.0 0.0 2.009099802785844 0.0 50-51 0.0 0.0 0.0 2.2634594779259904 0.0 52-53 0.0 0.0 0.0 2.5269810705467646 0.0 54-55 0.0 0.0 0.0 2.8291690606705284 0.0 56-57 0.0 0.0 0.0 3.2012360824262 0.0 58-59 0.0 0.0 0.0 3.538518875102877 0.0 60-61 0.0 0.0 0.0 3.9153997857043032 0.0 62-63 0.0 0.0 0.0 4.284671646194699 0.0 64-65 0.0 0.0 0.0 4.670093327328912 0.0 66-67 0.0 0.0 0.0 5.148531763902045 0.0 68-69 0.0 0.0 0.0 5.70088668726804 0.0 70-71 0.0 0.0 0.0 6.285230678447753 0.0 72-73 0.0 0.0 0.0 6.863829060359954 0.0 74-75 0.0 0.0 0.0 7.505629144214793 0.0 76-77 0.0 0.0 0.0 8.120254049101666 0.0 78-79 0.0 0.0 0.0 8.94078916719723 0.0 80-81 0.0 0.0 0.0 9.731509231796512 0.0 82-83 0.0 0.0 0.0 10.479370156994891 0.0 84-85 0.0 0.0 0.0 11.332049629641133 0.0 86-87 0.0 0.0 0.0 12.200568349457273 0.0 88-89 0.0 0.0 0.0 13.11784710467879 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 70 4.365782E-6 40.625072 1 CATTCCG 60 8.501235E-5 39.404503 9 GGCGCCC 25 0.0016023886 38.06203 84-85 CTCGAGG 30 0.003922744 31.710922 38-39 ATAAGAC 85 6.5686984E-4 27.814943 3 CTTTGCG 310 0.0 27.45604 2 GTACTGG 70 0.008472891 27.083382 1 TGGTAAC 70 0.008569942 27.020231 3 TTGCGTT 335 0.0 25.407084 4 CCTTTAG 115 1.2981298E-4 24.728306 1 ATAGCCC 115 1.3188747E-4 24.670645 3 GCATTCC 140 1.8375935E-5 23.642702 8 CGTAACG 70 1.8413039E-5 23.642702 14-15 TTAGGCA 100 0.0016951562 23.6427 4 GCTTTGC 385 0.0 23.390192 1 GGCATTC 125 2.3210775E-4 22.696995 7 TTTGCGT 395 0.0 21.547777 3 AGATATG 110 0.0029493903 21.493364 6 CGCAGAA 135 3.9053435E-4 21.015734 9 CCTTGGC 135 3.9053435E-4 21.015734 9 >>END_MODULE