FastQCFastQC Report
Thu 26 May 2016
SRR938921_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938921_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694267
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT102810.6068110870364588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77230.4558313418133033No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT69620.4109151627222864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65710.3878373361459557No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT45540.2687888036537335No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA34930.20616585225351142No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33940.20032261739147372No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32360.19099705064195902No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31850.18798689934939417No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30930.1825568225079046No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA30740.18143539359498828No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29440.17376245892766606No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27280.1610135828650384No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC27120.16006922167521412No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25250.14903200026914293No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT24570.14501846521238979No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG22790.13451244697559475No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA19830.11704176496384572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT61850.024.06968912-13
TTTGCGT5650.023.4691833
GTACATG107250.023.1006981
GTACTTT65300.022.47280514-15
TACATGG109300.022.1405872
CTTTGCG6050.021.9175032
ACATGGG108800.021.5023773
CATGGGG41200.020.6901534
AGTACTT64950.020.29717412-13
ACTTTTT76500.019.33993716-17
TACCTGG13050.019.2332972
GTAAGCG2001.8189894E-1219.02675294-95
TTGCGTT7000.018.9429844
ATGGGAG25150.018.8300045
TACACTG11950.018.6259445
CATGGGA63500.018.4955124
CGGTATA1051.930119E-518.12502134-35
CGGTTAC2002.3646862E-1117.797728-29
TACTTTT75000.017.35722214-15
GTATTAC3351.4726538E-817.0015451
Produced by FastQC (version 0.11.5)