##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938921_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1694267 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.208952307989236 33.0 31.0 34.0 31.0 34.0 2 32.45841416966747 34.0 31.0 34.0 31.0 34.0 3 32.52974531168936 34.0 31.0 34.0 31.0 34.0 4 36.06696465197044 37.0 35.0 37.0 35.0 37.0 5 36.00097623337998 37.0 35.0 37.0 35.0 37.0 6 36.05291019656288 37.0 35.0 37.0 35.0 37.0 7 35.99797139411911 37.0 35.0 37.0 35.0 37.0 8 35.97510250745603 37.0 35.0 37.0 35.0 37.0 9 37.64513444457101 39.0 37.0 39.0 35.0 39.0 10-11 37.56087883432777 39.0 37.0 39.0 35.0 39.0 12-13 37.47204248208813 39.0 37.0 39.0 34.5 39.0 14-15 38.756316153239126 40.0 38.0 41.0 34.5 41.0 16-17 38.85534688452292 40.0 38.0 41.0 35.0 41.0 18-19 38.93300377095228 40.0 38.0 41.0 35.0 41.0 20-21 38.916040387967186 40.0 38.0 41.0 35.0 41.0 22-23 38.84508669530835 40.0 38.0 41.0 34.5 41.0 24-25 38.761572408599115 40.0 38.0 41.0 34.0 41.0 26-27 38.59533680346722 40.0 38.0 41.0 34.0 41.0 28-29 38.45102778959868 40.0 38.0 41.0 34.0 41.0 30-31 38.26765202887148 40.0 38.0 41.0 34.0 41.0 32-33 38.14962193089991 40.0 38.0 41.0 33.0 41.0 34-35 38.04251159941143 40.0 37.0 41.0 33.0 41.0 36-37 37.90387140869768 40.0 37.0 41.0 33.0 41.0 38-39 37.75492440093563 40.0 37.0 41.0 33.0 41.0 40-41 37.60718233902921 40.0 37.0 41.0 33.0 41.0 42-43 37.422817950181404 40.0 37.0 41.0 32.0 41.0 44-45 37.153341238423465 40.0 36.5 41.0 31.0 41.0 46-47 36.803375737118174 40.0 36.0 41.0 31.0 41.0 48-49 36.430786587946294 39.0 36.0 41.0 30.0 41.0 50-51 36.160961347886726 39.0 35.0 41.0 29.5 41.0 52-53 35.909729104090445 39.0 35.0 40.0 28.5 41.0 54-55 35.61287742722959 39.0 35.0 40.0 28.0 41.0 56-57 35.47027269019582 38.5 35.0 40.0 28.0 41.0 58-59 35.19313071670521 38.0 34.5 40.0 27.5 41.0 60-61 34.81271842041426 38.0 34.0 40.0 26.5 41.0 62-63 34.39586706227531 37.0 34.0 40.0 26.0 41.0 64-65 33.984283764011224 37.0 33.0 39.5 26.0 41.0 66-67 33.509846736081144 36.0 33.0 39.0 24.5 40.5 68-69 33.01614798612025 35.5 32.0 39.0 23.0 40.0 70-71 32.46276885520405 35.0 32.0 38.0 22.0 40.0 72-73 31.940378936731932 35.0 31.0 37.0 20.5 39.0 74-75 31.421656681030797 35.0 31.0 37.0 19.5 39.0 76-77 29.523965526094763 32.5 28.5 35.0 16.0 37.0 78-79 30.481362441693072 34.0 30.0 36.0 18.0 37.5 80-81 30.447188371136306 34.0 30.0 35.0 17.0 37.0 82-83 30.03920751569853 34.0 30.0 35.0 10.5 37.0 84-85 29.577046002784684 34.0 29.5 35.0 7.0 36.0 86-87 28.910356218943058 34.0 29.0 35.0 2.0 36.0 88-89 28.210338748261048 33.0 27.5 35.0 2.0 35.0 90-91 27.617734394874006 33.0 26.5 35.0 2.0 35.0 92-93 27.220551129190383 33.0 26.0 34.5 2.0 35.0 94-95 26.657369529123805 32.0 25.0 34.0 2.0 35.0 96-97 25.7775504097052 32.0 24.0 34.0 2.0 35.0 98-99 24.554459539139934 32.0 10.5 34.0 2.0 35.0 100-101 18.87470894493017 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12315281029326286 1101 2 0.10910380813506038 1101 3 0.16297357240666344 1101 4 0.06981385021359898 1101 5 0.05680859290114171 1101 6 0.06428407520888868 1101 7 0.12781658227365966 1101 8 0.09840916741927686 1101 9 0.13157568155479993 1101 10-11 0.21717042930384167 1101 12-13 0.2614158792875969 1101 14-15 0.22456997195223494 1101 16-17 0.26824259418862084 1101 18-19 0.17446025276196764 1101 20-21 0.2517009219735229 1101 22-23 0.3376761765574159 1101 24-25 0.20002496614121412 1101 26-27 0.1391331519581982 1101 28-29 0.21937366117639812 1101 30-31 0.2264002674463299 1101 32-33 0.30023394140825843 1101 34-35 0.41197921033561613 1101 36-37 0.46036675429682106 1101 38-39 0.38959458513379985 1101 40-41 0.4464937863227689 1101 42-43 0.426741130431445 1101 44-45 0.43240251272348473 1101 46-47 0.30721841343380163 1101 48-49 0.4310660960104329 1101 50-51 0.5328947447229524 1101 52-53 0.590513850799681 1101 54-55 0.5894051516020795 1101 56-57 0.5011313529843449 1101 58-59 0.4626991485016063 1101 60-61 0.5101647191074292 1101 62-63 0.6616474470564313 1101 64-65 0.6650442032600594 1101 66-67 0.48435771936309635 1101 68-69 0.6865944357484679 1101 70-71 0.6243335762979427 1101 72-73 0.5639639090319442 1101 74-75 0.5833667120944313 1101 76-77 0.3198986234518131 1101 78-79 0.5446260066810389 1101 80-81 0.6541413658795285 1101 82-83 0.8254914793670203 1101 84-85 0.6772155962094466 1101 86-87 0.8679996357560107 1101 88-89 0.9060958394916874 1101 90-91 0.8507470114216531 1101 92-93 0.7830013075172211 1101 94-95 0.8919760099173359 1101 96-97 1.035627228645346 1101 98-99 1.0809263711339163 1101 100-101 1.2679017989893886 1102 1 0.07661921051928289 1102 2 0.11522252553761092 1102 3 0.12067149029680735 1102 4 0.1167704430324008 1102 5 0.08471780097562487 1102 6 0.14586697390959102 1102 7 0.08813261283708584 1102 8 0.12117897031880887 1102 9 0.12582321422763698 1102 10-11 0.2142713794112936 1102 12-13 0.24938398894516922 1102 14-15 0.29214509488210894 1102 16-17 0.29670831984786616 1102 18-19 0.27992856875761873 1102 20-21 0.3068892133990673 1102 22-23 0.23263323443048733 1102 24-25 0.20045510165770963 1102 26-27 0.21780866788273556 1102 28-29 0.2063854732373258 1102 30-31 0.17394337623093037 1102 32-33 0.308299974352515 1102 34-35 0.3056772091737656 1102 36-37 0.232748284499948 1102 38-39 0.3594075238006056 1102 40-41 0.34398880246475017 1102 42-43 0.40389474436339157 1102 44-45 0.2889130343778632 1102 46-47 0.4371092209140386 1102 48-49 0.4713299944667 1102 50-51 0.4337959262464821 1102 52-53 0.31654986631630777 1102 54-55 0.29483136669524157 1102 56-57 0.37875640972731617 1102 58-59 0.2409773842793541 1102 60-61 0.3650487412395691 1102 62-63 0.5412725942707155 1102 64-65 0.34375320944067767 1102 66-67 0.2321574412828724 1102 68-69 0.1587288761120007 1102 70-71 0.46679664178329716 1102 72-73 0.3368392617906899 1102 74-75 0.39989962936556367 1102 76-77 0.1807017705835534 1102 78-79 0.3384512208758288 1102 80-81 0.26591787317913074 1102 82-83 0.4200270520504006 1102 84-85 0.6232087886403299 1102 86-87 0.7158725283300633 1102 88-89 0.6179125827150678 1102 90-91 0.5128608814667643 1102 92-93 0.594123604091692 1102 94-95 0.6934244238124592 1102 96-97 0.6544321264762551 1102 98-99 0.8542241217315016 1102 100-101 1.1459722682021614 1103 1 0.09911699908759886 1103 2 0.11777900330723412 1103 3 0.13526145008325585 1103 4 0.1025051402387831 1103 5 0.060428909577311174 1103 6 0.0490752750909067 1103 7 0.09130938935387434 1103 8 0.08056626258538557 1103 9 0.0846898005926704 1103 10-11 0.10417344906041848 1103 12-13 0.06350766600714053 1103 14-15 0.16880719753018525 1103 16-17 0.12623477547180784 1103 18-19 0.1521066407655951 1103 20-21 0.144046284647942 1103 22-23 0.2523773200270014 1103 24-25 0.21056808856477716 1103 26-27 0.12743090516151767 1103 28-29 0.10355546803027238 1103 30-31 0.17519926554015086 1103 32-33 0.17958484753239645 1103 34-35 0.07431636312078638 1103 36-37 0.16941076356192752 1103 38-39 0.0014618183695844778 1103 40-41 0.13472580520629407 1103 42-43 0.1273544272974121 1103 44-45 0.04352959408024759 1103 46-47 0.06529423148499802 1103 48-49 0.04706015902231542 1103 50-51 -0.049643822434497054 1103 52-53 0.1642853445815362 1103 54-55 0.16609624288643232 1103 56-57 0.33157959243235524 1103 58-59 0.1645551670759815 1103 60-61 0.08732742554749251 1103 62-63 0.12273091584111029 1103 64-65 0.1908515287525958 1103 66-67 0.16350503442962605 1103 68-69 0.24905958709512532 1103 70-71 0.17259427853132792 1103 72-73 0.1315831121285136 1103 74-75 0.33887979862634765 1103 76-77 -0.2384954953967835 1103 78-79 0.0842726344355107 1103 80-81 0.1989475659626514 1103 82-83 0.3093319567235788 1103 84-85 0.38243837916473566 1103 86-87 0.5582797893542839 1103 88-89 0.34884329746233433 1103 90-91 0.31777574918864104 1103 92-93 0.47812734627326137 1103 94-95 0.7785214131285798 1103 96-97 0.6183093308501846 1103 98-99 0.9592181147929395 1103 100-101 1.3013582806614608 1104 1 0.0214940403567212 1104 2 0.053368630038747256 1104 3 0.03120743526362446 1104 4 0.034548759504772875 1104 5 0.09734575848789007 1104 6 0.13342576093080538 1104 7 0.15921437298919727 1104 8 0.11496033787310722 1104 9 0.24322709409207732 1104 10-11 0.10885823575461728 1104 12-13 0.04933834905496326 1104 14-15 0.2126722671987551 1104 16-17 0.2355265862269036 1104 18-19 0.15813540273895654 1104 20-21 0.23412833168060132 1104 22-23 0.1500354955790968 1104 24-25 0.1501596267975529 1104 26-27 0.07470905605868694 1104 28-29 0.18323862649577194 1104 30-31 0.014891714717840898 1104 32-33 0.023240366129122947 1104 34-35 0.2744792252251216 1104 36-37 0.21178453267987862 1104 38-39 0.09142694681132468 1104 40-41 0.24089259389203477 1104 42-43 0.11970351796110634 1104 44-45 0.1738661626843907 1104 46-47 0.4863014027534689 1104 48-49 0.2145217151695249 1104 50-51 0.23335409578683652 1104 52-53 0.22305066856766587 1104 54-55 0.3070396425181059 1104 56-57 0.19544888372521285 1104 58-59 0.3547063844025331 1104 60-61 0.3475697687529973 1104 62-63 0.41082028558712835 1104 64-65 0.35214092109764294 1104 66-67 0.11163457238148311 1104 68-69 0.08624554493695769 1104 70-71 0.1496572442930173 1104 72-73 0.2691346955907399 1104 74-75 0.20651520251730204 1104 76-77 0.06643740981049007 1104 78-79 0.35279403014530786 1104 80-81 0.4103104836229292 1104 82-83 0.4662139497696707 1104 84-85 0.40618893923617705 1104 86-87 0.5189186356738169 1104 88-89 0.687903091512986 1104 90-91 0.9167908866150682 1104 92-93 0.938770116035446 1104 94-95 1.0902612951585056 1104 96-97 1.1125948366329474 1104 98-99 1.4151480452459957 1104 100-101 1.8763664610393853 1105 1 -0.04057501120970386 1105 2 0.054546939169043185 1105 3 0.03614681979790646 1105 4 0.07290814145004987 1105 5 -0.05661073999224442 1105 6 -0.028525459722587243 1105 7 0.024347496186834405 1105 8 0.0764375834838873 1105 9 0.11540667744945665 1105 10-11 0.15331990990495825 1105 12-13 0.04370921308073861 1105 14-15 0.11151567607591772 1105 16-17 0.0577522179986687 1105 18-19 0.14187555555263742 1105 20-21 0.15971257337512412 1105 22-23 0.07708735989652382 1105 24-25 -0.05084638094139393 1105 26-27 0.03817445463285907 1105 28-29 0.13785734874389988 1105 30-31 0.06632791056514264 1105 32-33 0.138384796727415 1105 34-35 0.14173170622478892 1105 36-37 0.28086651211445 1105 38-39 0.288155637819834 1105 40-41 0.26831966895601767 1105 42-43 0.2872476129245598 1105 44-45 0.37346108480369367 1105 46-47 0.3187498994930422 1105 48-49 0.25987803865788806 1105 50-51 0.12210798904185793 1105 52-53 0.21459234296754914 1105 54-55 0.3419980312836941 1105 56-57 0.34981439818074733 1105 58-59 0.2987585356939988 1105 60-61 0.13532225521141328 1105 62-63 0.2689354120818521 1105 64-65 0.03085185402837709 1105 66-67 0.29886816612295064 1105 68-69 0.41798420985266205 1105 70-71 0.4735433093643806 1105 72-73 0.4980422993108 1105 74-75 0.3102547548690069 1105 76-77 0.14248338336208732 1105 78-79 0.27803671035797706 1105 80-81 0.33504140647838554 1105 82-83 0.41123853751743766 1105 84-85 0.4594814983426865 1105 86-87 0.4296274069414814 1105 88-89 0.6910527874608796 1105 90-91 0.813696635921417 1105 92-93 0.8837315383036959 1105 94-95 0.744386429872538 1105 96-97 0.764396495467615 1105 98-99 0.875254366004981 1105 100-101 1.3248536478048898 1106 1 0.013393337954042295 1106 2 0.08368265093803018 1106 3 0.027167338115965833 1106 4 0.06258676026812537 1106 5 0.044392685974635526 1106 6 0.03228395306518905 1106 7 0.050224416265102434 1106 8 0.04886004943037392 1106 9 0.14108418676404 1106 10-11 0.11147719701897785 1106 12-13 0.049187386598092075 1106 14-15 0.03129913547783758 1106 16-17 -0.10665802091941146 1106 18-19 -0.07252193906852966 1106 20-21 -0.03507969458495808 1106 22-23 0.1414061397425428 1106 24-25 0.20665832320349864 1106 26-27 0.12101313432370375 1106 28-29 0.08951410734442078 1106 30-31 0.1610885051990394 1106 32-33 0.16865025293576963 1106 34-35 0.08064248115300643 1106 36-37 0.037739061648530026 1106 38-39 0.03674720559325095 1106 40-41 0.17307778969698262 1106 42-43 0.0509274911790385 1106 44-45 0.09313846667530612 1106 46-47 0.12038416815781261 1106 48-49 0.1457494861851174 1106 50-51 0.1461137464654101 1106 52-53 0.10912091643092481 1106 54-55 0.11587540865731683 1106 56-57 0.15305444825833803 1106 58-59 0.0328813907163763 1106 60-61 0.04780997945480436 1106 62-63 0.1769984288818378 1106 64-65 0.05539943148746573 1106 66-67 0.10549201445082446 1106 68-69 0.1452676879961956 1106 70-71 0.19116717268553884 1106 72-73 0.1593358079656717 1106 74-75 0.027997065185143555 1106 76-77 -0.13708694641533725 1106 78-79 0.30302958854499096 1106 80-81 0.1964891586093067 1106 82-83 0.07564559036128671 1106 84-85 0.269462906662465 1106 86-87 0.5414190676733028 1106 88-89 0.6451883118137332 1106 90-91 0.8375640802400852 1106 92-93 0.8140910774099446 1106 94-95 0.682457580532617 1106 96-97 0.7538140436148204 1106 98-99 0.7644430790558907 1106 100-101 1.163735935481153 1107 1 0.03031502914450357 1107 2 0.017271763931141493 1107 3 0.014071830329299928 1107 4 -0.008543062105765387 1107 5 -0.004562960962438467 1107 6 0.0754097085787393 1107 7 0.061239373394521124 1107 8 0.09000819442373142 1107 9 -0.02007838434711573 1107 10-11 0.08435138351098459 1107 12-13 0.1443725791668271 1107 14-15 0.13653331522799306 1107 16-17 0.03186750660348281 1107 18-19 0.04715684420805388 1107 20-21 0.10514488889713647 1107 22-23 0.1816285484330109 1107 24-25 0.11017767975515369 1107 26-27 0.12084476920518483 1107 28-29 0.07980928093810036 1107 30-31 0.13335519989635003 1107 32-33 0.0873868077396125 1107 34-35 0.11203559829996834 1107 36-37 0.14726519267610882 1107 38-39 0.1490190110508962 1107 40-41 0.19219221454615365 1107 42-43 0.07103217114939042 1107 44-45 0.06453868814158881 1107 46-47 -0.0637048750607363 1107 48-49 -0.02618174690361741 1107 50-51 -0.23073831514643928 1107 52-53 -0.22046766736288959 1107 54-55 -0.3255960553580337 1107 56-57 -0.34969557575498555 1107 58-59 -0.26424933419604457 1107 60-61 -0.12254670449540583 1107 62-63 -0.11006409818978824 1107 64-65 -0.031389978002238195 1107 66-67 -0.15633006222144985 1107 68-69 -0.16966135036531682 1107 70-71 0.04367642914240122 1107 72-73 0.051504745779315186 1107 74-75 -0.05565122026350622 1107 76-77 -0.021700839346721068 1107 78-79 0.037937893892983965 1107 80-81 0.01867837635488101 1107 82-83 -0.08312326009822257 1107 84-85 0.058057936901128926 1107 86-87 -0.046860980244606054 1107 88-89 0.28160936597148023 1107 90-91 0.4055136323236255 1107 92-93 0.5614631135767283 1107 94-95 0.5209231200531121 1107 96-97 0.4578122167898506 1107 98-99 0.6894087807795906 1107 100-101 0.8719548355077968 1108 1 0.08095255642329136 1108 2 0.09825369137304563 1108 3 0.14216065909263165 1108 4 0.12784166845280964 1108 5 0.1049569736997853 1108 6 0.20062481565113188 1108 7 0.09774014078325877 1108 8 0.1388505835444107 1108 9 0.22430538430253222 1108 10-11 0.18643284339161426 1108 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-1.2244813612818817 2316 52-53 -1.1178477352263627 2316 54-55 -1.0997798898851343 2316 56-57 -1.0473881641584768 2316 58-59 -1.1292291889935555 2316 60-61 -0.9131643370422751 2316 62-63 -1.007021989705187 2316 64-65 -1.0531535479456622 2316 66-67 -0.9264687960017213 2316 68-69 -0.7371975208704313 2316 70-71 -0.916578619500811 2316 72-73 -0.9397422700629328 2316 74-75 -1.2869051182196642 2316 76-77 -1.0494895304929734 2316 78-79 -1.2040619460993547 2316 80-81 -1.2924229135507481 2316 82-83 -1.2814729803376181 2316 84-85 -1.4432777404353914 2316 86-87 -1.760429719149279 2316 88-89 -1.8230057546335487 2316 90-91 -1.8076008097359022 2316 92-93 -1.8869647516682662 2316 94-95 -1.658668527309036 2316 96-97 -1.759137799255523 2316 98-99 -1.8702349672184546 2316 100-101 -1.0009257693330618 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 15.0 5 38.0 6 86.0 7 199.0 8 441.0 9 920.0 10 1547.0 11 2389.0 12 3427.0 13 4686.0 14 5922.0 15 8144.0 16 11079.0 17 16481.0 18 16351.0 19 10777.0 20 10107.0 21 10799.0 22 11443.0 23 12649.0 24 14519.0 25 17498.0 26 20610.0 27 24790.0 28 29860.0 29 36417.0 30 43589.0 31 53348.0 32 66709.0 33 84409.0 34 114511.0 35 165430.0 36 258770.0 37 381109.0 38 243769.0 39 11427.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.24729874598286 20.59394698650099 16.434078023914093 20.724676243602058 2 15.405246044454623 22.323577098532876 38.5173057139164 23.7538711430961 3 19.235220894935686 26.506743034008217 31.399419335913407 22.85861673514269 4 12.878253545633598 18.5948259630861 38.250877813237224 30.27604267804307 5 13.11469797853585 38.78745203678051 33.80346781233418 14.29438217234946 6 29.15703369067567 37.3477734028934 17.78904977786854 15.706143128562381 7 26.683633689377178 34.45696575569258 21.285074902597998 17.57432565233225 8 26.484668591196076 33.146546559662674 19.992185411154203 20.376599437987046 9 26.5902599767333 17.33026730733704 21.2804711417976 34.79900157413206 10-11 25.04466533314997 26.888766646579317 26.866987316638998 21.199580703631717 12-13 26.93748978171681 24.78325435129174 27.098149229135664 21.18110663785578 14-15 23.487498483846437 25.673622233259525 27.05653135346549 23.78234792942855 16-17 21.318154774552717 29.091322794044732 27.416758743784797 22.173763687617754 18-19 21.292087364472422 28.01963218717805 29.516245602427254 21.172034845922273 20-21 23.157507051722074 26.156562100306502 28.93953550414427 21.746395343827153 22-23 22.478452333664055 26.22724163310741 29.228657584666408 22.065648448562122 24-25 22.74889111207515 26.678795820016347 29.18864522374009 21.383667844168418 26-27 22.310090440290697 26.169163419933223 30.016904065297854 21.503842074478225 28-29 21.481679097804538 27.15094492190428 29.718072771292835 21.649303208998347 30-31 21.95719446816824 26.531857139400106 29.25197150154019 22.25897689089146 32-33 21.945803111315985 26.655656989128634 29.392297672090645 22.00624222746474 34-35 22.753398789534486 26.443175827996544 29.02812121655739 21.775304165911578 36-37 22.671915034300355 26.02641304476167 29.699620984024655 21.602050936913322 38-39 22.301894766915748 26.53517794540658 29.527320615765305 21.635606671912363 40-41 22.063721795302378 26.701548893651605 29.452412587350672 21.78231672369534 42-43 21.897792123585123 26.835506994663216 28.63188749513409 22.634813386617573 44-45 22.257593755302903 26.514907148496004 28.626122370682978 22.601376725518115 46-47 22.83327976976223 26.93019943650533 28.185387208547034 22.051133585185408 48-49 22.746612529707598 26.806382754557184 28.154110899902996 22.292893815832226 50-51 21.964065642972493 27.17706178995382 28.618904108942218 22.239968458131475 52-53 22.49952339271201 27.56177155076502 27.629736045145187 22.308969011377783 54-55 21.95678131014769 26.855153290479013 28.124758376336196 23.0633070230371 56-57 22.59416609070471 27.187391361574058 27.65650278261927 22.56193976510196 58-59 23.232784684945504 26.655883162771595 27.745624993175465 22.365707159107444 60-61 22.49640620467177 26.93671703439803 28.620422319276585 21.946454441653614 62-63 22.454603673022497 26.90460942779822 28.45010609445505 22.190680804724227 64-65 22.15442775733287 27.319146173391847 28.1363785262549 22.390047543020383 66-67 22.244959512604833 27.109290291583616 27.53638671468058 23.109363481130966 68-69 21.95362654336928 27.083115067167938 28.14121041291187 22.82204797655091 70-71 22.676958635775023 27.105513538015874 27.56267315757974 22.654854668629365 72-73 22.368788390495713 26.615226525689277 28.205501258066175 22.810483825748832 74-75 21.907881107287107 27.90690605435861 27.444818319662723 22.740394518691566 76-77 22.285858434584622 26.873750697482624 27.71699205024888 23.123398817683878 78-79 22.195713566034346 26.982322982660605 28.016258948515986 22.805704502789066 80-81 22.4294804202534 27.329397109717586 27.630560417550164 22.61056205247885 82-83 22.24035526867961 27.078288132862177 28.03826079360573 22.643095804852482 84-85 21.945045164153875 27.1543572902452 27.71463006945789 23.185967476143034 86-87 22.064955937969025 27.186537486179386 27.776079278276544 22.972427297575045 88-89 22.17703412396367 26.922416811000154 27.420467382608187 23.480081682427993 90-91 21.960702979053735 26.665234595481863 27.99688302299363 23.37717940247077 92-93 22.360761320382206 27.255149276943953 27.678400157708317 22.70568924496552 94-95 21.928782077974894 26.913729699356736 27.672472281991357 23.485015940677012 96-97 21.412770242234547 26.955993358779935 27.96489573367126 23.666340665314262 98-99 21.66361618328162 27.150443230022187 27.200848508529056 23.985092078167135 100-101 21.799376069604453 26.07100624195998 28.35371441033679 23.77590327809878 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 303.0 1 222.0 2 188.5 3 258.0 4 233.5 5 223.5 6 449.5 7 978.5 8 1641.5 9 2076.5 10 1998.0 11 1755.0 12 1772.5 13 1926.5 14 2098.0 15 2279.0 16 2318.5 17 2334.5 18 2519.5 19 2673.5 20 2876.0 21 3227.0 22 3703.0 23 4330.0 24 5014.0 25 6025.5 26 7748.5 27 9774.0 28 11423.0 29 13612.5 30 16703.5 31 20194.0 32 24082.0 33 28658.0 34 33613.0 35 37281.0 36 42212.0 37 47978.5 38 50812.0 39 51600.5 40 51781.5 41 52927.5 42 54189.0 43 56038.5 44 58577.0 45 60330.0 46 60778.5 47 70502.0 48 96838.0 49 95323.5 50 69679.5 51 58309.5 52 52972.5 53 51170.0 54 49559.0 55 45917.5 56 42332.5 57 38461.5 58 34719.5 59 30885.5 60 26171.0 61 21177.5 62 16847.0 63 13333.0 64 10240.0 65 7683.5 66 5482.0 67 4101.0 68 2968.0 69 1879.0 70 1303.0 71 903.5 72 603.5 73 422.0 74 322.0 75 263.5 76 163.0 77 71.5 78 36.0 79 18.0 80 6.0 81 2.0 82 2.5 83 2.0 84 2.0 85 2.5 86 1.5 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4019437314189558 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.377821795502126E-4 16-17 0.002065790102740595 18-19 5.016918820941445E-4 20-21 0.0 22-23 0.0 24-25 1.18045148728034E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012276695467715537 36-37 0.02402218776615492 38-39 0.010624063385523061 40-41 0.0053120316927615305 42-43 0.004869362385031403 44-45 0.0017411659437385019 46-47 0.0010919176257343146 48-49 0.0026855271335627737 50-51 6.492483180041871E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.377821795502126E-4 60-61 0.0016231207950104676 62-63 0.0012984966360083741 64-65 7.082708923682041E-4 66-67 0.002124812677104612 68-69 0.0025674819848347397 70-71 5.9022574364017E-5 72-73 0.0 74-75 0.0 76-77 0.09281299818741674 78-79 0.003629888323387046 80-81 2.0657901027405952E-4 82-83 0.0 84-85 1.7706772309205102E-4 86-87 0.09422953997215315 88-89 0.44210859327366936 90-91 0.39748752705447254 92-93 0.0 94-95 9.738724770062805E-4 96-97 0.0 98-99 0.0 100-101 4.426693077301276E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1694267.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.66991529469909 #Duplication Level Percentage of deduplicated Percentage of total 1 71.90682149229306 32.83978446664072 2 14.887149171396526 13.597896832744583 3 5.3114298482975215 7.277176537964528 4 2.487167008191103 4.543548263514312 5 1.3758383312650506 3.14172100240375 6 0.8514700874401635 2.3331940061617376 7 0.5582625699248931 1.784706299646963 8 0.39475829280445396 1.4422862235407545 9 0.29976554220828505 1.2321240228829733 >10 1.6908991599231031 14.474845979748935 >50 0.15325870351035284 4.868611205866649 >100 0.07593574513516252 5.980388214650386 >500 0.003233949826034759 1.0548911585184175 >1k 0.0034926658121175393 3.5463744038251614 >5k 3.88073979124171E-4 1.2140464441954233 >10k+ 1.2935799304139036E-4 0.6684049376947455 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11314 0.6677814063544885 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7298 0.43074674770859606 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6847 0.40412756667042443 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6405 0.37803958880152894 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4024 0.2375068392408044 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3637 0.21466510296192987 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3568 0.2105925453308127 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3473 0.20498540076623106 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3426 0.20221133977112227 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3345 0.1974305112476369 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3024 0.17848426487678742 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3023 0.1784252423024234 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2992 0.17659554249713888 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2954 0.17435268467130624 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2554 0.15074365492569944 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2536 0.14968124858714713 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2296 0.13551583073978304 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2239 0.13215154400103407 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1750 0.10328950513702975 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.9022574364017005E-5 0.0 5 0.0 0.0 0.0 5.9022574364017005E-5 0.0 6 0.0 0.0 0.0 5.9022574364017005E-5 0.0 7 0.0 0.0 0.0 5.9022574364017005E-5 0.0 8 0.0 0.0 0.0 5.9022574364017005E-5 0.0 9 0.0 0.0 0.0 1.1804514872803401E-4 0.0 10-11 0.0 0.0 0.0 2.3609029745606802E-4 2.9511287182008503E-5 12-13 0.0 0.0 0.0 2.3609029745606802E-4 1.1804514872803401E-4 14-15 0.0 0.0 0.0 2.3609029745606802E-4 2.3609029745606802E-4 16-17 0.0 0.0 0.0 3.8364673336611053E-4 2.95112871820085E-4 18-19 0.0 0.0 0.0 4.1315802054811904E-4 2.95112871820085E-4 20-21 0.0 0.0 0.0 5.9022574364017E-4 2.95112871820085E-4 22-23 0.0 0.0 0.0 6.787596051861956E-4 2.95112871820085E-4 24-25 5.9022574364017005E-5 0.0 0.0 9.443611898242721E-4 2.95112871820085E-4 26-27 5.9022574364017005E-5 0.0 0.0 0.0012099627744623486 3.2462415900209353E-4 28-29 5.9022574364017005E-5 0.0 0.0 0.00236090297456068 3.5413544618410203E-4 30-31 5.9022574364017005E-5 0.0 0.0 0.0058432348620376835 3.5413544618410203E-4 32-33 5.9022574364017005E-5 0.0 0.0 0.01083064239579712 3.5413544618410203E-4 34-35 8.853386154602551E-5 0.0 0.0 0.019064291519577493 4.1315802054811904E-4 36-37 1.1804514872803401E-4 0.0 0.0 0.03305264164384952 4.1315802054811904E-4 38-39 1.1804514872803401E-4 0.0 0.0 0.05539268604062996 4.1315802054811904E-4 40-41 1.1804514872803401E-4 0.0 0.0 0.08319231856608197 4.1315802054811904E-4 42-43 1.7706772309205102E-4 0.0 0.0 0.11352992178918672 4.1315802054811904E-4 44-45 3.5413544618410203E-4 0.0 0.0 0.15307504661307808 4.4266930773012754E-4 46-47 3.5413544618410203E-4 0.0 0.0 0.2051329572021411 5.31203169276153E-4 48-49 3.5413544618410203E-4 0.0 0.0 0.2644801557251602 5.9022574364017E-4 50-51 3.5413544618410203E-4 0.0 0.0 0.3308215293103153 5.9022574364017E-4 52-53 3.5413544618410203E-4 0.0 0.0 0.3998484300290332 5.9022574364017E-4 54-55 3.5413544618410203E-4 0.0 0.0 0.4752792800662469 5.9022574364017E-4 56-57 3.5413544618410203E-4 0.0 0.0 0.5732862647976972 5.9022574364017E-4 58-59 3.5413544618410203E-4 0.0 0.0 0.6808844178633002 5.9022574364017E-4 60-61 3.5413544618410203E-4 0.0 0.0 0.8092880283922192 5.9022574364017E-4 62-63 3.5413544618410203E-4 0.0 0.0 0.9437119415062679 5.9022574364017E-4 64-65 5.31203169276153E-4 0.0 0.0 1.0839200669079903 5.9022574364017E-4 66-67 5.31203169276153E-4 0.0 0.0 1.256649630784286 5.9022574364017E-4 68-69 5.31203169276153E-4 0.0 0.0 1.4570312707501238 5.9022574364017E-4 70-71 5.31203169276153E-4 0.0 0.0 1.6793397970921937 5.9022574364017E-4 72-73 5.31203169276153E-4 0.0 0.0 1.9078161824553037 5.9022574364017E-4 74-75 5.31203169276153E-4 0.0 0.0 2.1507235872504156 5.9022574364017E-4 76-77 5.31203169276153E-4 0.0 0.0 2.4216372035812537 5.9022574364017E-4 78-79 5.31203169276153E-4 0.0 0.0 2.7399754584135794 5.9022574364017E-4 80-81 6.492483180041871E-4 0.0 0.0 3.1003672974802674 5.9022574364017E-4 82-83 6.492483180041871E-4 0.0 0.0 3.457689962680026 5.9022574364017E-4 84-85 6.492483180041871E-4 0.0 0.0 3.844819027933614 5.9022574364017E-4 86-87 6.492483180041871E-4 0.0 0.0 4.254199603722435 5.9022574364017E-4 88-89 6.492483180041871E-4 0.0 0.0 4.69447849719082 5.9022574364017E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 6310 0.0 23.763208 12-13 CGCAACG 100 0.0016644615 23.724207 7 GTAAGCG 195 0.0 23.116575 94-95 GTACATG 9965 0.0 21.991194 1 TTTGCGT 610 0.0 21.7796 3 GTACTTT 6925 0.0 21.515785 14-15 TACATGG 10230 0.0 21.103645 2 CTTTGCG 675 0.0 20.385244 2 ACATGGG 10215 0.0 20.019838 3 AGTACTT 6730 0.0 19.953484 12-13 TTGCGTT 725 0.0 18.324903 4 ACTTTTT 8285 0.0 18.212482 16-17 CATGGGA 5985 0.0 17.599913 4 CATGGGG 4065 0.0 16.808294 4 TACTTTT 8190 0.0 16.42542 14-15 ATGGGAG 2365 0.0 16.050203 5 ATAACGC 215 4.8288182E-4 15.448321 3 AGAGTAC 17325 0.0 15.200823 10-11 ATGGGGA 2165 0.0 15.122128 5 ATTAGAC 380 6.9700036E-8 14.983709 3 >>END_MODULE