FastQCFastQC Report
Thu 26 May 2016
SRR938920_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938920_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703824
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104620.6140305571467475No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76500.4489900365295946No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT70760.41530111091286426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67560.39651982833907734No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT45510.26710505310407645No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35250.20688756585187204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35040.20565504418296726No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA34430.2020748621923391No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA31860.18699114462526645No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC31180.1830001220783367No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31140.1827653560461644No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30460.17877433349923466No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27370.16063865751392162No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC26570.15594333687047487No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25300.14848951534900318No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT25070.14713961066401227No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG22660.13299495722562893No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA20270.11896768680333179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT5850.026.6973933
GAGTACT63550.023.94146312-13
GTATAGG6000.023.7074781
CTTTGCG6650.023.4856782
GTACTTT67500.022.1902214-15
GTACATG107200.021.5844211
GGACCGT1554.022858E-521.3710636
TTGCGTT7650.021.034314
TACATGG110600.020.7112
CATGGGG43800.019.8817464
AGTACTT66250.019.85839712-13
ATGGGGA22300.019.7373525
ACTTTTT78000.019.62980516-17
ACATGGG111750.019.1849863
ATCACGC1300.00772523918.2027683
GAACCGT2352.6213893E-618.123186
CCTATAC4450.018.0800533
ATGGGAT26700.016.4847555
CATGGGA65700.016.4240514
GATTGGC6350.016.395869
Produced by FastQC (version 0.11.5)