##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938920_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1703824 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71968818375607 33.0 31.0 34.0 30.0 34.0 2 31.785251293560837 33.0 31.0 34.0 30.0 34.0 3 31.812988900262 34.0 31.0 34.0 30.0 34.0 4 35.351538069659775 37.0 35.0 37.0 33.0 37.0 5 35.395794988214746 37.0 35.0 37.0 33.0 37.0 6 35.4903517029928 37.0 35.0 37.0 33.0 37.0 7 35.41056881461935 37.0 35.0 37.0 33.0 37.0 8 35.3909106809154 37.0 35.0 37.0 33.0 37.0 9 36.96631107438327 39.0 37.0 39.0 33.0 39.0 10-11 36.851574751852304 39.0 37.0 39.0 33.0 39.0 12-13 36.72706864089248 39.0 37.0 39.0 32.0 39.0 14-15 37.86998803867066 40.0 38.0 41.0 32.0 41.0 16-17 37.80137971997108 40.0 38.0 41.0 32.0 41.0 18-19 37.78406836621623 40.0 38.0 41.0 32.0 41.0 20-21 37.681762024716164 40.0 37.5 41.0 32.0 41.0 22-23 37.76988350909484 40.0 38.0 41.0 32.0 41.0 24-25 37.69100036153969 40.0 38.0 41.0 32.0 41.0 26-27 37.59341633877678 40.0 37.0 41.0 32.0 41.0 28-29 37.47628951112321 40.0 37.0 41.0 32.0 41.0 30-31 37.30058004817399 40.0 37.0 41.0 31.0 41.0 32-33 37.09529123900121 40.0 36.5 41.0 31.0 41.0 34-35 36.76271404792983 40.0 36.0 41.0 30.0 41.0 36-37 36.482778150794914 39.0 35.5 41.0 30.0 41.0 38-39 36.272212681591526 39.0 35.0 41.0 30.0 41.0 40-41 36.13764596578051 39.0 35.0 41.0 29.5 41.0 42-43 35.924394773169055 39.0 35.0 41.0 28.5 41.0 44-45 35.80539275183352 39.0 35.0 41.0 27.5 41.0 46-47 35.52674131835213 39.0 35.0 41.0 26.0 41.0 48-49 35.29259096009916 39.0 35.0 41.0 25.0 41.0 50-51 34.12055147714787 38.0 34.0 40.0 23.5 40.5 52-53 34.26999472950258 38.0 33.5 39.5 23.5 40.5 54-55 34.76704489430833 39.0 34.0 40.0 24.0 41.0 56-57 34.659814628740996 38.5 34.0 40.5 23.0 41.0 58-59 34.45830261811079 38.0 34.0 40.0 23.0 41.0 60-61 34.17230711622797 38.0 34.0 40.0 22.5 41.0 62-63 33.82074351576219 37.5 33.5 40.0 22.0 41.0 64-65 33.41262301740086 37.0 33.0 40.0 20.5 41.0 66-67 33.00533271042079 36.0 33.0 39.0 19.5 41.0 68-69 32.60738961301168 36.0 32.0 39.0 18.0 41.0 70-71 32.16506663833823 35.0 32.0 38.5 16.0 40.0 72-73 31.7209019241424 35.0 31.0 37.5 13.0 40.0 74-75 31.263372566650077 35.0 31.0 37.0 9.0 39.0 76-77 30.85546658575064 35.0 31.0 36.5 7.0 39.0 78-79 30.426743607320944 35.0 30.5 36.0 4.5 38.5 80-81 29.980797605856004 34.0 30.0 35.5 2.0 37.0 82-83 29.594987510447087 34.0 29.5 35.0 2.0 37.0 84-85 29.24666192048005 34.0 29.0 35.0 2.0 36.5 86-87 28.881787085990105 34.0 29.0 35.0 2.0 36.0 88-89 28.45642096836293 34.0 29.0 35.0 2.0 36.0 90-91 28.02499524598785 34.0 27.5 35.0 2.0 35.0 92-93 27.61215507000723 34.0 27.0 35.0 2.0 35.0 94-95 27.138325026528562 33.5 26.0 35.0 2.0 35.0 96-97 26.54416624017504 33.0 25.0 35.0 2.0 35.0 98-99 25.824545258195684 33.0 23.5 35.0 2.0 35.0 100-101 23.10361310792664 29.5 9.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34427297040012306 1101 2 0.39765456175639713 1101 3 0.34200708548272374 1101 4 0.18330152120548604 1101 5 0.03319742221925992 1101 6 0.08200081019463568 1101 7 0.18264063368433625 1101 8 0.16731108302116837 1101 9 0.12199750574754376 1101 10-11 0.45048495406973643 1101 12-13 0.520562121953823 1101 14-15 0.18527765689036357 1101 16-17 0.40676059785448615 1101 18-19 0.3384930556987982 1101 20-21 0.5479163058413619 1101 22-23 0.484711295216421 1101 24-25 0.4922847196241733 1101 26-27 0.4848640933159629 1101 28-29 0.42013325932629897 1101 30-31 0.465587269146404 1101 32-33 0.429397213253381 1101 34-35 0.43358769545545783 1101 36-37 0.49932396264470924 1101 38-39 0.4551699995582652 1101 40-41 0.41625373479839567 1101 42-43 0.31109738081342897 1101 44-45 0.47667160321100255 1101 46-47 0.4436771281181322 1101 48-49 0.605585452383373 1101 50-51 0.4089830563103263 1101 52-53 0.5376651771659979 1101 54-55 0.3978820975230022 1101 56-57 0.46842442672655693 1101 58-59 0.45404100781566115 1101 60-61 0.4004828893719079 1101 62-63 0.5317694923465695 1101 64-65 0.32761433832052944 1101 66-67 0.3605823242052111 1101 68-69 0.3734087126722656 1101 70-71 0.26450242959527515 1101 72-73 0.2865315338720542 1101 74-75 0.1925933153722319 1101 76-77 0.2639509369134281 1101 78-79 0.17375887189373884 1101 80-81 0.05463528485209679 1101 82-83 0.10203318232100855 1101 84-85 0.09972269574929982 1101 86-87 -0.15932489736819733 1101 88-89 -0.05270279199176997 1101 90-91 -0.11025939873478663 1101 92-93 -0.06265239675438394 1101 94-95 -0.007064815032585159 1101 96-97 -0.10256302478364532 1101 98-99 -0.15776783714229836 1101 100-101 -0.2314086045709729 1102 1 0.3533248287366497 1102 2 0.3193965993034027 1102 3 0.28094102878348437 1102 4 0.2536695045026107 1102 5 0.3083307185593469 1102 6 0.3444679384727607 1102 7 0.38145064575884646 1102 8 0.3029154803930396 1102 9 0.467073707635727 1102 10-11 0.35187419240317297 1102 12-13 0.3309389591226193 1102 14-15 0.5645632352277943 1102 16-17 0.5764294239927068 1102 18-19 0.5866666574515023 1102 20-21 0.6459028542368515 1102 22-23 0.5411650668623551 1102 24-25 0.55993277975697 1102 26-27 0.6490930731255418 1102 28-29 0.6071525337300656 1102 30-31 0.539372149935069 1102 32-33 0.6256672900392743 1102 34-35 0.6450696198858168 1102 36-37 0.6257934008948922 1102 38-39 0.6084748641710007 1102 40-41 0.5909302448870974 1102 42-43 0.6206512521420109 1102 44-45 0.8532619588479022 1102 46-47 0.8153160938848245 1102 48-49 0.7982921521831301 1102 50-51 0.7111346390356275 1102 52-53 0.5817765674497863 1102 54-55 0.7591757814420816 1102 56-57 0.7504688745409851 1102 58-59 0.7273127699773809 1102 60-61 0.7424100693410267 1102 62-63 0.6996028251054724 1102 64-65 0.7263391816640592 1102 66-67 0.7467206536956112 1102 68-69 0.8870299653342641 1102 70-71 0.8565204651463247 1102 72-73 0.7634812142403966 1102 74-75 0.7073063668292292 1102 76-77 0.6486267691754506 1102 78-79 0.5488019062289169 1102 80-81 0.5184828052510433 1102 82-83 0.49653730997913215 1102 84-85 0.44896561696018367 1102 86-87 0.3281462231941923 1102 88-89 0.31382796468108864 1102 90-91 0.39034298621945496 1102 92-93 0.21113654333531073 1102 94-95 0.10866796347854901 1102 96-97 0.19099451982940963 1102 98-99 0.19036394615076446 1102 100-101 0.34184320574325255 1103 1 0.12888408558855957 1103 2 0.22540285539700022 1103 3 0.3585554078684474 1103 4 0.2886760946156315 1103 5 0.2303969898192264 1103 6 0.3006143473125391 1103 7 0.2941598592785084 1103 8 0.16189270387911847 1103 9 0.3387854217873212 1103 10-11 0.3594105057314536 1103 12-13 0.39877932040524655 1103 14-15 0.4114119424823315 1103 16-17 0.5879051353638118 1103 18-19 0.5310710421335543 1103 20-21 0.509171550297232 1103 22-23 0.5548925882576512 1103 24-25 0.629828601805869 1103 26-27 0.3957884248389689 1103 28-29 0.3121953243269715 1103 30-31 0.42712977370280925 1103 32-33 0.44932308753323014 1103 34-35 0.6540255157800914 1103 36-37 0.5210185029062302 1103 38-39 0.5847013699666874 1103 40-41 0.6498367149336772 1103 42-43 0.33981708376011 1103 44-45 0.49010497874462544 1103 46-47 0.5887939357582255 1103 48-49 0.49074658561395523 1103 50-51 0.32591939962494365 1103 52-53 0.4630603079257227 1103 54-55 0.5895858805646057 1103 56-57 0.5915405126805879 1103 58-59 0.5945394834067699 1103 60-61 0.31601790796450047 1103 62-63 0.2984223598254374 1103 64-65 0.4150285616517806 1103 66-67 0.4547933164623714 1103 68-69 0.43106523598028446 1103 70-71 0.472188233174343 1103 72-73 0.3724826992727124 1103 74-75 0.333808890030042 1103 76-77 0.27690038525171445 1103 78-79 0.20652678255144963 1103 80-81 0.3216889403754415 1103 82-83 0.3620555591756798 1103 84-85 0.23342096217377417 1103 86-87 0.30531554396117144 1103 88-89 0.3377402574987727 1103 90-91 0.14316861002330583 1103 92-93 0.13079979307351053 1103 94-95 0.014660114022003512 1103 96-97 -0.05469933782414671 1103 98-99 -0.08772415373006837 1103 100-101 0.4015096828313247 1104 1 0.15583429230503043 1104 2 0.19049905108859377 1104 3 0.18277689671059605 1104 4 0.22923150913656087 1104 5 0.17830912322992987 1104 6 0.12621545705359694 1104 7 0.06932774085777282 1104 8 0.2677827633554344 1104 9 0.2137990215552179 1104 10-11 0.24653205441257597 1104 12-13 0.37018920834316305 1104 14-15 0.36411119419287985 1104 16-17 0.24934382458796023 1104 18-19 0.3701899433931928 1104 20-21 0.3291166676783348 1104 22-23 0.3164788349634904 1104 24-25 0.38710296654559784 1104 26-27 0.3134482523334654 1104 28-29 0.4091646916053264 1104 30-31 0.4276288096303702 1104 32-33 0.4424688045129841 1104 34-35 0.356370040211317 1104 36-37 0.4489435107638684 1104 38-39 0.43401707497866227 1104 40-41 0.44235903366388385 1104 42-43 0.3775870391528713 1104 44-45 0.43622647604885145 1104 46-47 0.5759591548051475 1104 48-49 0.5057316409801231 1104 50-51 0.5115248764025893 1104 52-53 0.4189558828634645 1104 54-55 0.48952823265957335 1104 56-57 0.47901338869906596 1104 58-59 0.4709013490531575 1104 60-61 0.46386589580328774 1104 62-63 0.46349218707815965 1104 64-65 0.5169089562263807 1104 66-67 0.6737609127487332 1104 68-69 0.7108978379483233 1104 70-71 0.6626469382346656 1104 72-73 0.5897794129643579 1104 74-75 0.6676095264991808 1104 76-77 0.6913933556827985 1104 78-79 0.5113278028362629 1104 80-81 0.548933506512622 1104 82-83 0.43988287536726034 1104 84-85 0.44452298158820014 1104 86-87 0.6569209210071811 1104 88-89 0.5066610856944109 1104 90-91 0.5517116819993184 1104 92-93 0.7491075739540491 1104 94-95 0.4940863095927135 1104 96-97 0.745722557197734 1104 98-99 0.7025666697509898 1104 100-101 1.1456595160075054 1105 1 0.16043825401852274 1105 2 0.320492959784076 1105 3 0.2309809968090626 1105 4 0.19953270344621643 1105 5 0.22473240384095305 1105 6 0.36581218903916124 1105 7 0.23279277647416308 1105 8 0.3425202606572242 1105 9 0.42944524686843266 1105 10-11 0.29425454226692693 1105 12-13 0.4339372234945458 1105 14-15 0.5233415164701967 1105 16-17 0.5008710861326051 1105 18-19 0.4388749460692267 1105 20-21 0.5515435350623576 1105 22-23 0.4205074493099872 1105 24-25 0.5269050582877384 1105 26-27 0.373246899235923 1105 28-29 0.6102627527371851 1105 30-31 0.6033204388177751 1105 32-33 0.47709498766018044 1105 34-35 0.4706141566321165 1105 36-37 0.5778891828328767 1105 38-39 0.5213175182245564 1105 40-41 0.42531853535969333 1105 42-43 0.4853528610206581 1105 44-45 0.24880243254629164 1105 46-47 0.2734131521498142 1105 48-49 0.3052545380755163 1105 50-51 0.32640650993669595 1105 52-53 0.31997551105733635 1105 54-55 0.39015939234341346 1105 56-57 0.35429134142935226 1105 58-59 0.0663906789209463 1105 60-61 0.3456258392665106 1105 62-63 0.3899836696011505 1105 64-65 0.3242012235222802 1105 66-67 0.37260171680092213 1105 68-69 0.29635388741207436 1105 70-71 0.1829637304236087 1105 72-73 0.23714758501118993 1105 74-75 0.34662143411242496 1105 76-77 0.1965602455987323 1105 78-79 0.24172215566472488 1105 80-81 0.08462271100599494 1105 82-83 0.24164233093474508 1105 84-85 0.3764264107560855 1105 86-87 0.3932687108394326 1105 88-89 0.4216356411918163 1105 90-91 0.5953480429475526 1105 92-93 0.5879803721396719 1105 94-95 0.7162995377771182 1105 96-97 0.5689076589313906 1105 98-99 0.6976078747647811 1105 100-101 0.9964499071224786 1106 1 0.27063245160163163 1106 2 0.31751886846889477 1106 3 0.3142149444103346 1106 4 0.20515708094956153 1106 5 0.2867138347390181 1106 6 0.17735974244028796 1106 7 0.265435111803761 1106 8 0.26210437945843523 1106 9 0.3972004544670611 1106 10-11 0.290903746967345 1106 12-13 0.34245319146882736 1106 14-15 0.3673128634567817 1106 16-17 0.3527874140328535 1106 18-19 0.32542078960829457 1106 20-21 0.5047152223114892 1106 22-23 0.3315748520722721 1106 24-25 0.2989462488646879 1106 26-27 0.3795878158074757 1106 28-29 0.2974894419740295 1106 30-31 0.3440471088186712 1106 32-33 0.4193171457625766 1106 34-35 0.2975285609375504 1106 36-37 0.21975216303694367 1106 38-39 0.22916995285439157 1106 40-41 0.19041715165382556 1106 42-43 0.06849169754501361 1106 44-45 0.1425670999567501 1106 46-47 0.17965660658412475 1106 48-49 0.3167362617874616 1106 50-51 0.2681263960598841 1106 52-53 0.25117119622043305 1106 54-55 0.061093233505779665 1106 56-57 0.4351512524310337 1106 58-59 0.5399401815648233 1106 60-61 0.6175163482497013 1106 62-63 0.4606976866228223 1106 64-65 0.32285684448005725 1106 66-67 0.6022420686905363 1106 68-69 0.5549325859505672 1106 70-71 0.5180512753609179 1106 72-73 0.46819162678311343 1106 74-75 0.3725696594893435 1106 76-77 0.3742154476403101 1106 78-79 0.4114435977623856 1106 80-81 0.4914379005655789 1106 82-83 0.5152325496688448 1106 84-85 0.560171927711508 1106 86-87 0.403503303913638 1106 88-89 0.5250267251161205 1106 90-91 0.5573100241647175 1106 92-93 0.5091327550461777 1106 94-95 0.5643574550796409 1106 96-97 0.6496975724551177 1106 98-99 0.6599919781772385 1106 100-101 1.0365921248990588 1107 1 0.10883955677167734 1107 2 0.06246915871785319 1107 3 0.16269130970092505 1107 4 0.2284462769300788 1107 5 0.11860873644530301 1107 6 0.10560393684258429 1107 7 0.06817574909666035 1107 8 0.1695113744883443 1107 9 0.1840958987443102 1107 10-11 0.1480395095340441 1107 12-13 0.15400150520421363 1107 14-15 0.07823935905277324 1107 16-17 0.22661280091070068 1107 18-19 0.2845515873855575 1107 20-21 0.0966136657513843 1107 22-23 0.11343799482003902 1107 24-25 0.18716637927258972 1107 26-27 0.16932990195925868 1107 28-29 0.30976038247396076 1107 30-31 0.45403861502041565 1107 32-33 0.2617421658129615 1107 34-35 0.20010296296955943 1107 36-37 0.18228419151361663 1107 38-39 0.19859705093390545 1107 40-41 0.3639896686493742 1107 42-43 0.28479742196915936 1107 44-45 0.3995728929180302 1107 46-47 0.5235103379348018 1107 48-49 0.41745793419264743 1107 50-51 0.40488715195490954 1107 52-53 0.3699509382111543 1107 54-55 0.3836894939016702 1107 56-57 0.31604561439409196 1107 58-59 0.44153601179207413 1107 60-61 0.29637707646831046 1107 62-63 0.36948602845172474 1107 64-65 0.29642356462620256 1107 66-67 0.2514556235624852 1107 68-69 0.2577436691024886 1107 70-71 0.35860840207474354 1107 72-73 0.2150580585359947 1107 74-75 0.3602927100601363 1107 76-77 0.4364142279690526 1107 78-79 0.3904961168645933 1107 80-81 0.43049657705295274 1107 82-83 0.5508384600516756 1107 84-85 0.5103414678834923 1107 86-87 0.647499234808123 1107 88-89 0.6529699075086839 1107 90-91 0.5512182070185538 1107 92-93 0.617601351144689 1107 94-95 0.8186853863855639 1107 96-97 0.8329609017624691 1107 98-99 0.8956646583682399 1107 100-101 0.7947553436774584 1108 1 0.12712577356809973 1108 2 0.13728300600796928 1108 3 0.11367049738251467 1108 4 0.17889703806547885 1108 5 0.13098131775330302 1108 6 0.2695153134961146 1108 7 0.2606009647061853 1108 8 0.25681491189275363 1108 9 0.41073412880636084 1108 10-11 0.3981866140008492 1108 12-13 0.3888732746536192 1108 14-15 0.3269508029540589 1108 16-17 0.4034555403083999 1108 18-19 0.29434274574253294 1108 20-21 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quality scores pass #Quality Count 2 5908.0 3 1586.0 4 1613.0 5 1732.0 6 2093.0 7 2533.0 8 3166.0 9 3892.0 10 4808.0 11 5971.0 12 7727.0 13 11111.0 14 10793.0 15 10622.0 16 13223.0 17 17545.0 18 15481.0 19 10743.0 20 10889.0 21 11347.0 22 12343.0 23 13724.0 24 15572.0 25 17712.0 26 20363.0 27 23689.0 28 28190.0 29 32982.0 30 39733.0 31 47359.0 32 59091.0 33 76103.0 34 102298.0 35 144916.0 36 222267.0 37 340207.0 38 309452.0 39 45036.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52264325976295 19.853997240521945 15.891132469073597 20.73222703064151 2 15.14457094553117 21.79440561165896 39.07994593238729 23.981077510422583 3 19.191291975425408 26.47325944663697 31.258408009183164 23.077040568754455 4 12.587133604076184 18.097408671291856 38.77703903209431 30.53841869253765 5 13.149050382267848 38.613870694051755 33.791455438121496 14.445623485558901 6 29.01697209147413 37.05241417471862 18.080067763834577 15.850545969972679 7 26.58113030196217 34.5105745505816 21.42145458818 17.48684055927623 8 26.34675135393297 32.813202190260846 20.335576817448302 20.504469638357882 9 26.73236672972306 17.30034426441387 21.48374437486047 34.483544631002594 10-11 25.00241584985486 26.738273729152834 26.962323609917053 21.296986811075254 12-13 27.082072726119154 24.796853759380387 27.04832272171315 21.07275079278731 14-15 23.705145751795843 25.515487312539054 27.117022398518962 23.662344537146137 16-17 21.344389569908834 28.992778453673836 27.46798827680969 22.19484369960764 18-19 21.284843499845778 27.914839240338996 29.591616115623577 21.20870114419165 20-21 23.21849284565246 26.091394924449574 28.956982260132243 21.733129969765727 22-23 22.514323757914756 26.147847095334924 29.280431870739637 22.057397276010686 24-25 22.784598857067635 26.560659807191872 29.16986955714309 21.4848717785974 26-27 22.289521288537465 26.101011680103625 30.056167228711168 21.55329980264774 28-29 21.49504810271786 27.06178221289544 29.703215251361748 21.73995443302495 30-31 21.98192671437516 26.45326827256068 29.319215286696064 22.24558972636809 32-33 21.989575710070657 26.591546283383096 29.358645576786518 22.06023242975973 34-35 22.800510374983023 26.346561818448034 29.03701927340208 21.815908533166862 36-37 22.638625246134907 25.965601631944544 29.743606396703047 21.6521667252175 38-39 22.341038466238366 26.468480423901358 29.535106397422872 21.655374712437403 40-41 22.038171569657244 26.59195733300602 29.58665464424895 21.783216453087785 42-43 21.90652651063846 26.733226712124804 28.645413846807223 22.714832930429512 44-45 22.233669225809958 26.501036724611467 28.63877940436747 22.626514645211113 46-47 22.849273842877096 26.79422970437772 28.28591853769748 22.070577915047703 48-49 22.700094501801914 26.752571491159316 28.139513119394955 22.407820887643823 50-51 22.01211334720589 27.042337674682503 28.73128082838598 22.214268149725626 52-53 22.512923005163298 27.42286788865698 27.72558061268772 22.338628493492003 54-55 21.955776851034088 26.752649563365182 28.257168226020507 23.034405359580227 56-57 22.60952783412548 27.070259174510912 27.70147803777398 22.618734953589627 58-59 23.1571703801672 26.64681923165108 27.759497245526582 22.436513142655137 60-61 22.54347517690754 26.755663356449116 28.771139613987206 21.929721852656144 62-63 22.451393771106677 26.8054859501332 28.59419281223418 22.14892746652594 64-65 22.186257170948323 27.201447918911814 28.194589200249194 22.41770570989067 66-67 22.267604698031167 26.989111257114594 27.67592148293605 23.067362561918188 68-69 21.948486257834475 26.969429864387013 28.304505422304903 22.777578455473613 70-71 22.69038677661352 26.980168345535322 27.651548482462974 22.677896395388185 72-73 22.32903042936525 26.55040361869106 28.338933407344857 22.781632544598832 74-75 21.98033208176001 27.7858135536622 27.602225845948713 22.631628518629075 76-77 22.351679435757056 26.77399728470481 27.77143579129836 23.102887488239773 78-79 22.295894410236688 26.889400914858648 28.103349100414576 22.71135557449009 80-81 22.56627008790367 27.23744732892192 27.666666076431866 22.52961650674255 82-83 22.362867330192802 27.007018973069343 28.11261053849514 22.517503158242718 84-85 22.178438835093605 27.006583054796014 27.800499286080534 23.014478824029847 86-87 22.225996971673638 27.18383357782297 27.81582698988492 22.774342460618474 88-89 22.402090483923295 26.85186688515357 27.487165468993584 23.258877161929554 90-91 22.19210768956877 26.6299859849327 28.070582559729374 23.107323765769163 92-93 22.615794803960068 27.08215105069372 27.845878570099885 22.456175575246323 94-95 22.269926586261143 26.848010175881175 27.798308271564697 23.083754966292986 96-97 21.784586113974218 26.787746766347038 28.182626361878015 23.245040757800727 98-99 22.096458974243138 27.059806241136506 27.374411204483778 23.46932358013657 100-101 22.32476774491985 26.18564406634063 28.164568639195263 23.32501954954426 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1644.0 1 949.0 2 303.5 3 369.5 4 321.0 5 309.0 6 586.0 7 1138.5 8 1912.5 9 2513.5 10 2443.0 11 2186.0 12 2293.5 13 2513.0 14 2704.5 15 2881.5 16 3051.5 17 3136.0 18 3102.5 19 3097.0 20 3273.5 21 3599.5 22 3967.5 23 4497.0 24 5161.5 25 6176.5 26 7777.0 27 9620.0 28 11313.5 29 13533.5 30 16581.5 31 19996.0 32 23820.0 33 28415.5 34 33426.5 35 37378.5 36 42564.0 37 48114.0 38 50615.5 39 51550.0 40 51841.5 41 52846.5 42 54193.5 43 55968.5 44 58448.0 45 60259.0 46 61183.5 47 70038.0 48 96176.0 49 95139.5 50 68984.5 51 57730.0 52 52560.0 53 51369.0 54 49738.5 55 45800.5 56 42559.5 57 38738.5 58 34521.5 59 30608.0 60 26224.5 61 21420.0 62 17199.0 63 13705.5 64 10453.0 65 7814.5 66 5752.5 67 4364.0 68 3080.5 69 2055.0 70 1490.0 71 1056.5 72 736.5 73 544.0 74 405.5 75 273.5 76 182.5 77 109.5 78 68.5 79 41.5 80 24.0 81 14.5 82 11.5 83 10.5 84 9.5 85 11.5 86 13.5 87 10.5 88 7.0 89 4.0 90 1.5 91 1.0 92 2.0 93 2.5 94 1.0 95 0.0 96 1.0 97 1.0 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2906403478293533 2 0.08868286865310032 3 0.09261519969198696 4 0.10799237479927504 5 0.11010526908882608 6 0.08457446309008441 7 0.09531500906196884 8 0.09161744405525453 9 0.09648883922283052 10-11 0.08947520401168195 12-13 0.09402379588502098 14-15 0.10385462348223759 16-17 0.0913826780230822 18-19 0.10265144756735438 20-21 0.10458826733277615 22-23 0.11846880898496559 24-25 0.1312342119843364 26-27 0.13487308548300764 28-29 0.12636281681676043 30-31 0.1191731070814826 32-33 0.11348003080130342 34-35 0.38974095916010104 36-37 0.5950732000488314 38-39 0.5778178966841646 40-41 0.5406661720928922 42-43 0.5672240794823878 44-45 0.5885584426560491 46-47 0.5774070561278629 48-49 0.5989761853336965 50-51 0.5911702147639663 52-53 0.5950732000488314 54-55 0.5845674201091192 56-57 0.556571570772568 58-59 0.5516121383429274 60-61 0.4999049197569702 62-63 0.5723008949281146 64-65 0.6079853318183098 66-67 0.6198703621970344 68-69 0.6213376498981116 70-71 0.6174933561212895 72-73 0.6100395345998177 74-75 0.6077799115401591 76-77 0.5919332043685264 78-79 0.5682805266271634 80-81 0.5624407215768764 82-83 0.5777592051761214 84-85 0.5872378837250796 86-87 0.5762919174750444 88-89 0.5900550761111476 90-91 0.6039062720093155 92-93 0.6115068223008949 94-95 0.610861215712421 96-97 0.6071343049516852 98-99 0.6121817746433904 100-101 0.5898790015870183 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1703824.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.606803001969325 #Duplication Level Percentage of deduplicated Percentage of total 1 73.70594546074291 35.08904425623483 2 13.924945728452363 13.258442962150918 3 5.02202127777573 7.172471329283024 4 2.3281844291602942 4.433496698851461 5 1.3092654815032339 3.116497192760149 6 0.7592198048259978 2.1686416610126926 7 0.5308512432495164 1.7690491392511158 8 0.3660939861191825 1.394285142190529 9 0.2903304171848474 1.2439532678758742 >10 1.5489079427209547 13.829737365001895 >50 0.13761164166468254 4.546286423253884 >100 0.0696797502748029 5.6014170533684045 >500 0.00297550128234342 0.9558110625608031 >1k 0.00347141816273399 3.5354503302082314 >5k 3.719376602929275E-4 1.2679222703427917 >10k+ 1.239792200976425E-4 0.6174938456533976 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10462 0.6140305571467475 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7650 0.4489900365295946 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7076 0.41530111091286426 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6756 0.39651982833907734 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4551 0.26710505310407645 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3525 0.20688756585187204 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3504 0.20565504418296726 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3443 0.2020748621923391 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3186 0.18699114462526645 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3118 0.1830001220783367 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3114 0.1827653560461644 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3046 0.17877433349923466 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2737 0.16063865751392162 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2657 0.15594333687047487 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2530 0.14848951534900318 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2507 0.14713961066401227 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2266 0.13299495722562893 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2027 0.11896768680333179 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1738301608616853E-4 0.0 12-13 0.0 0.0 0.0 3.2280329423696344E-4 0.0 14-15 0.0 0.0 0.0 4.1084055630158985E-4 1.1738301608616853E-4 16-17 0.0 0.0 0.0 5.282235723877584E-4 2.3476603217233705E-4 18-19 2.9345754021542132E-5 0.0 0.0 5.869150804308426E-4 2.3476603217233705E-4 20-21 5.8691508043084264E-5 0.0 0.0 6.162608344523848E-4 2.3476603217233705E-4 22-23 5.8691508043084264E-5 0.0 0.0 6.74952342495469E-4 2.3476603217233705E-4 24-25 1.1738301608616853E-4 0.0 0.0 8.510268666247218E-4 2.3476603217233705E-4 26-27 1.1738301608616853E-4 0.0 0.0 0.0012325216689047696 2.934575402154213E-4 28-29 1.1738301608616853E-4 0.0 0.0 0.0023183145677018284 2.934575402154213E-4 30-31 1.1738301608616853E-4 0.0 0.0 0.006456065884739268 2.934575402154213E-4 32-33 1.4672877010771067E-4 0.0 0.0 0.012090450656875359 2.934575402154213E-4 34-35 1.760745241292528E-4 0.0 0.0 0.021422400435725755 2.934575402154213E-4 36-37 1.760745241292528E-4 0.0 0.0 0.03588985716834603 2.934575402154213E-4 38-39 1.760745241292528E-4 0.0 0.0 0.058574125026998096 2.934575402154213E-4 40-41 1.760745241292528E-4 0.0 0.0 0.08768511301636789 2.934575402154213E-4 42-43 1.760745241292528E-4 0.0 0.0 0.11981871366995653 2.934575402154213E-4 44-45 2.3476603217233705E-4 0.0 0.0 0.1609027693001155 3.5214904825850554E-4 46-47 2.9345754021542134E-4 0.0 0.0 0.21401858407910676 4.988778183662162E-4 48-49 3.521490482585056E-4 0.0 0.0 0.2722112143038248 5.869150804308426E-4 50-51 3.521490482585056E-4 0.0 0.0 0.33917822498098393 5.869150804308426E-4 52-53 3.521490482585056E-4 0.0 0.0 0.40608654415010004 5.869150804308426E-4 54-55 3.521490482585056E-4 0.0 0.0 0.48288438242447573 5.869150804308426E-4 56-57 3.521490482585056E-4 0.0 0.0 0.5808405093483835 5.869150804308426E-4 58-59 3.521490482585056E-4 0.0 0.0 0.6915033477636188 5.869150804308426E-4 60-61 3.8149480228004775E-4 0.0 0.0 0.8240581186789246 5.869150804308426E-4 62-63 4.1084055630158985E-4 0.0 0.0 0.9587257838837814 5.869150804308426E-4 64-65 4.1084055630158985E-4 0.0 0.0 1.1010233451342393 6.456065884739269E-4 66-67 4.695320643446741E-4 0.0 0.0 1.2710819896890757 6.456065884739269E-4 68-69 4.695320643446741E-4 0.0 0.0 1.4688723717942698 6.456065884739269E-4 70-71 4.695320643446741E-4 0.0 0.0 1.6895524420362666 6.456065884739269E-4 72-73 4.695320643446741E-4 0.0 0.0 1.9221468884110096 6.456065884739269E-4 74-75 4.695320643446741E-4 0.0 0.0 2.171527106086075 6.456065884739269E-4 76-77 4.695320643446741E-4 0.0 0.0 2.4407157077256807 6.456065884739269E-4 78-79 4.695320643446741E-4 0.0 0.0 2.7595573251697356 6.456065884739269E-4 80-81 5.282235723877584E-4 0.0 0.0 3.112263942754651 6.456065884739269E-4 82-83 5.282235723877584E-4 0.0 0.0 3.4718374667806065 6.456065884739269E-4 84-85 5.282235723877584E-4 0.0 0.0 3.8636326287222156 6.456065884739269E-4 86-87 5.282235723877584E-4 0.0 0.0 4.276762153837485 6.74952342495469E-4 88-89 5.282235723877584E-4 0.0 0.0 4.7243729399280685 7.042980965170112E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 585 0.0 26.697393 3 GAGTACT 6355 0.0 23.941463 12-13 GTATAGG 600 0.0 23.707478 1 CTTTGCG 665 0.0 23.485678 2 GTACTTT 6750 0.0 22.19022 14-15 GTACATG 10720 0.0 21.584421 1 GGACCGT 155 4.022858E-5 21.371063 6 TTGCGTT 765 0.0 21.03431 4 TACATGG 11060 0.0 20.711 2 CATGGGG 4380 0.0 19.881746 4 AGTACTT 6625 0.0 19.858397 12-13 ATGGGGA 2230 0.0 19.737352 5 ACTTTTT 7800 0.0 19.629805 16-17 ACATGGG 11175 0.0 19.184986 3 ATCACGC 130 0.007725239 18.202768 3 GAACCGT 235 2.6213893E-6 18.12318 6 CCTATAC 445 0.0 18.080053 3 ATGGGAT 2670 0.0 16.484755 5 CATGGGA 6570 0.0 16.424051 4 GATTGGC 635 0.0 16.39586 9 >>END_MODULE