##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938920_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1703824 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.183717919221706 33.0 31.0 34.0 30.0 34.0 2 32.4228429696964 34.0 31.0 34.0 31.0 34.0 3 32.5004079059809 34.0 31.0 34.0 30.0 34.0 4 36.06295427227225 37.0 35.0 37.0 35.0 37.0 5 35.99071735108791 37.0 35.0 37.0 35.0 37.0 6 36.03847169660716 37.0 35.0 37.0 35.0 37.0 7 35.9932369775282 37.0 35.0 37.0 35.0 37.0 8 35.96331722055799 37.0 35.0 37.0 35.0 37.0 9 37.63950736695809 39.0 37.0 39.0 35.0 39.0 10-11 37.56235033665449 39.0 37.0 39.0 35.0 39.0 12-13 37.49385294490511 39.0 37.0 39.0 35.0 39.0 14-15 38.72261072739907 40.0 38.0 41.0 34.0 41.0 16-17 38.715335034604514 40.0 38.0 41.0 34.5 41.0 18-19 38.86309442759346 40.0 38.0 41.0 35.0 41.0 20-21 38.87221919634892 40.0 38.0 41.0 34.5 41.0 22-23 38.816903623848475 40.0 38.0 41.0 34.0 41.0 24-25 38.74508194508353 40.0 38.0 41.0 34.0 41.0 26-27 38.58461730789095 40.0 38.0 41.0 34.0 41.0 28-29 38.42006040530008 40.0 38.0 41.0 34.0 41.0 30-31 38.263116965132554 40.0 38.0 41.0 34.0 41.0 32-33 38.09685478077548 40.0 37.0 41.0 33.0 41.0 34-35 38.0510865558884 40.0 37.0 41.0 33.0 41.0 36-37 37.94183290058128 40.0 37.0 41.0 33.0 41.0 38-39 37.817452682906215 40.0 37.0 41.0 33.0 41.0 40-41 37.68080652696523 40.0 37.0 41.0 33.0 41.0 42-43 37.50561325582924 40.0 37.0 41.0 32.5 41.0 44-45 37.24038075528928 40.0 37.0 41.0 31.5 41.0 46-47 36.897966867469876 40.0 36.0 41.0 31.0 41.0 48-49 36.574236834320914 40.0 36.0 41.0 30.0 41.0 50-51 36.30423036651673 39.0 36.0 41.0 30.0 41.0 52-53 36.07737917766154 39.0 35.0 41.0 29.0 41.0 54-55 35.83781482124914 39.0 35.0 40.0 28.5 41.0 56-57 35.56473115767825 38.5 35.0 40.0 28.0 41.0 58-59 35.26279504221093 38.0 34.5 40.0 28.0 41.0 60-61 34.836130668425845 38.0 34.0 40.0 26.5 41.0 62-63 34.53500097427903 37.0 34.0 40.0 26.0 41.0 64-65 34.279729596484145 37.0 34.0 39.5 26.0 41.0 66-67 33.81040177858746 36.5 33.0 39.0 25.5 41.0 68-69 33.390503068392036 36.0 33.0 39.0 25.0 40.0 70-71 32.909669954173665 35.0 32.5 38.5 24.0 40.0 72-73 32.3849649376931 35.0 32.0 37.5 22.5 39.5 74-75 31.927898069284154 35.0 31.5 37.0 21.5 39.0 76-77 29.957528477119702 33.0 29.0 35.0 19.0 37.5 78-79 30.926404370404455 34.0 31.0 36.0 20.0 38.0 80-81 30.916909845148325 34.0 31.0 35.5 19.5 37.0 82-83 30.602167242626 34.0 31.0 35.0 18.0 37.0 84-85 30.20371030106396 34.0 30.5 35.0 15.5 36.0 86-87 29.665328109006566 34.0 30.0 35.0 7.5 36.0 88-89 29.096193327479835 34.0 29.0 35.0 2.0 35.5 90-91 28.629894578313255 34.0 29.0 35.0 2.0 35.0 92-93 28.297920735944558 34.0 29.0 35.0 2.0 35.0 94-95 27.921065790832856 33.0 28.0 35.0 2.0 35.0 96-97 27.309199483045198 33.0 27.0 35.0 2.0 35.0 98-99 26.41910872249716 33.0 25.5 34.5 2.0 35.0 100-101 22.0244359159162 28.5 9.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06171027613558522 1101 2 0.1172653095113887 1101 3 0.14441706049925074 1101 4 0.06947052158802336 1101 5 0.10113187165661941 1101 6 0.05663115142149877 1101 7 0.1366117671818614 1101 8 0.1779347710014605 1101 9 0.17522491742002444 1101 10-11 0.21908817449520512 1101 12-13 0.25960557515416127 1101 14-15 0.30749024320453344 1101 16-17 0.2528190001880475 1101 18-19 0.2680919453409203 1101 20-21 0.1166849707961859 1101 22-23 0.14037660066436075 1101 24-25 0.15306647420482022 1101 26-27 0.13752809164709134 1101 28-29 0.20672776222498612 1101 30-31 0.25627188755080255 1101 32-33 0.2686850591041434 1101 34-35 0.3683937993146671 1101 36-37 0.40245121565053665 1101 38-39 0.3976024275494723 1101 40-41 0.4187739982874419 1101 42-43 0.36325579684979203 1101 44-45 0.43267087364669266 1101 46-47 0.47691164393455665 1101 48-49 0.2587093699839045 1101 50-51 0.3259137112923298 1101 52-53 0.3918144390101901 1101 54-55 0.44724003341046625 1101 56-57 0.25094806573243034 1101 58-59 0.3361726180122844 1101 60-61 0.2797527631939758 1101 62-63 0.28246789934559047 1101 64-65 0.10248241007726477 1101 66-67 0.358497522776986 1101 68-69 0.3407218752995078 1101 70-71 0.35058566980884365 1101 72-73 0.23757638280523707 1101 74-75 0.24551894799023088 1101 76-77 0.11866320422375765 1101 78-79 0.5502083174251986 1101 80-81 0.6074407309908771 1101 82-83 0.4485821974974975 1101 84-85 0.3266833832212903 1101 86-87 0.4272534112897013 1101 88-89 0.5399367527325438 1101 90-91 0.5233116550639032 1101 92-93 0.5148442914276607 1101 94-95 0.4513300349808169 1101 96-97 0.4254603913966797 1101 98-99 0.38178784687663025 1101 100-101 0.8113070621069092 1102 1 0.17839182052346558 1102 2 0.08445275386353757 1102 3 0.13817990617649656 1102 4 0.12738985408575587 1102 5 0.10553812803517815 1102 6 0.11506823627659202 1102 7 0.07903326278919565 1102 8 0.11900903821145903 1102 9 0.22204244456277422 1102 10-11 0.2105908319970453 1102 12-13 0.13804444456989984 1102 14-15 0.21278598434674478 1102 16-17 0.21841891318114648 1102 18-19 0.1618349540565518 1102 20-21 0.20280974375296523 1102 22-23 0.15222114322133962 1102 24-25 0.0958354995934485 1102 26-27 0.02738508553554908 1102 28-29 0.15222360393062928 1102 30-31 0.1210088927133981 1102 32-33 0.15122324812740828 1102 34-35 0.20392068701410437 1102 36-37 0.10355664756973937 1102 38-39 0.2657184178638303 1102 40-41 0.16256106573732154 1102 42-43 0.012239995063161757 1102 44-45 -0.008834215606228213 1102 46-47 0.12711190916775195 1102 48-49 0.03024769572136421 1102 50-51 0.06147475839286187 1102 52-53 0.07999827326509745 1102 54-55 0.18522785320966761 1102 56-57 0.17226004714235899 1102 58-59 0.036266862727629245 1102 60-61 -0.16310759646774642 1102 62-63 -0.07111272966991322 1102 64-65 -0.10078900359729204 1102 66-67 0.0018314420282976585 1102 68-69 0.07862199887841115 1102 70-71 0.1643056860307297 1102 72-73 0.157301215809035 1102 74-75 0.008890036652640987 1102 76-77 -0.14732517621852992 1102 78-79 -0.17186455315547633 1102 80-81 0.17190894149397096 1102 82-83 0.13212023831798092 1102 84-85 0.11736897235798693 1102 86-87 0.23791286771865927 1102 88-89 0.3891771847493999 1102 90-91 0.4406305863035591 1102 92-93 0.2433883005695101 1102 94-95 0.194991685347091 1102 96-97 0.21217820525628284 1102 98-99 0.25527896447496445 1102 100-101 0.9052251186054896 1103 1 0.02511414204875706 1103 2 0.04366288819351638 1103 3 0.05487027016739887 1103 4 0.1215106607878127 1103 5 0.10716872963125468 1103 6 0.07677223187114635 1103 7 0.08990017861630406 1103 8 0.10351663038276371 1103 9 0.0562040558002721 1103 10-11 0.10772356860918109 1103 12-13 0.0961046944525421 1103 14-15 0.13811515146478115 1103 16-17 0.1213947328828695 1103 18-19 0.14155568673267993 1103 20-21 0.17904640554476003 1103 22-23 0.1905521293911292 1103 24-25 0.1723880128639479 1103 26-27 0.1570354033053789 1103 28-29 0.24090406921066432 1103 30-31 0.310842514928396 1103 32-33 0.19945633049479738 1103 34-35 0.25611122252849583 1103 36-37 0.06300036981001256 1103 38-39 0.16567057183775802 1103 40-41 0.09818446334682562 1103 42-43 0.20643223227111918 1103 44-45 0.255737381909384 1103 46-47 0.12089756799850448 1103 48-49 -0.030642014674761242 1103 50-51 0.02217218192949133 1103 52-53 0.0732575129646591 1103 54-55 0.07884548672716818 1103 56-57 -0.061016976834821435 1103 58-59 0.13743468943474113 1103 60-61 -0.05741414505403242 1103 62-63 0.04198895667681768 1103 64-65 0.11394204305710076 1103 66-67 0.13574671393816828 1103 68-69 0.2778245274957598 1103 70-71 0.23462241769577474 1103 72-73 0.22400692663848787 1103 74-75 0.1845605580840477 1103 76-77 0.1667269985187687 1103 78-79 0.22194322317789172 1103 80-81 0.2516842908602399 1103 82-83 0.2514672980442647 1103 84-85 0.1660917994261979 1103 86-87 0.14706071193921844 1103 88-89 0.3297290615774777 1103 90-91 0.5841398891959848 1103 92-93 0.5300367767187204 1103 94-95 0.28907796166240374 1103 96-97 0.17834350424880796 1103 98-99 0.5931046334165764 1103 100-101 1.1096145401199493 1104 1 0.07215347257751858 1104 2 0.15391091773825138 1104 3 0.0836647565192763 1104 4 0.008640142666877182 1104 5 0.10849168483059657 1104 6 0.07447915989513376 1104 7 0.03440859233937488 1104 8 0.12065007130536998 1104 9 0.05507527506479448 1104 10-11 0.17609753502681968 1104 12-13 0.16642909424944463 1104 14-15 0.21752079627511733 1104 16-17 0.27645513096319263 1104 18-19 0.16628837573922794 1104 20-21 0.09686489699805634 1104 22-23 0.21502566726497463 1104 24-25 0.24256054858600606 1104 26-27 0.2557810513796497 1104 28-29 0.21673539280051557 1104 30-31 0.23308055317698262 1104 32-33 0.2741137674523131 1104 34-35 0.344133453615747 1104 36-37 0.3646160222362198 1104 38-39 0.39712955493777713 1104 40-41 0.4624867348102839 1104 42-43 0.31753138062342856 1104 44-45 0.42407455339943567 1104 46-47 0.5237400705975688 1104 48-49 0.5277002340619745 1104 50-51 0.5381481314955039 1104 52-53 0.47036129128240134 1104 54-55 0.4822504908373162 1104 56-57 0.5357941042560697 1104 58-59 0.6318810731053546 1104 60-61 0.7026051061729603 1104 62-63 0.4947373186603272 1104 64-65 0.5828140631968424 1104 66-67 0.5056645562292132 1104 68-69 0.6614025810969721 1104 70-71 0.391844045557761 1104 72-73 0.2536748203064363 1104 74-75 0.4216808223426476 1104 76-77 0.3075182179041782 1104 78-79 0.5543253283010721 1104 80-81 0.5931268635736657 1104 82-83 0.6763206472137746 1104 84-85 0.5145926516783206 1104 86-87 0.2813561532970219 1104 88-89 0.4610299095985617 1104 90-91 0.5785364070509651 1104 92-93 0.41906191513413305 1104 94-95 0.5180821866569651 1104 96-97 0.31175231629396905 1104 98-99 0.15081524848874395 1104 100-101 0.9766503649307587 1105 1 -0.006224022713546162 1105 2 0.05783537373449121 1105 3 0.07792976374900462 1105 4 0.03522380791716273 1105 5 0.05313695505091687 1105 6 0.0266461562610516 1105 7 0.09555153988023335 1105 8 0.04785380769973102 1105 9 -0.00783001106388781 1105 10-11 0.11290986075194098 1105 12-13 0.07791630398843807 1105 14-15 0.10294402366114497 1105 16-17 0.0367852146151364 1105 18-19 0.042083793658896695 1105 20-21 0.1853259407401282 1105 22-23 0.07622006563037331 1105 24-25 0.12679360603646472 1105 26-27 0.059556033859756496 1105 28-29 0.08444467790017995 1105 30-31 0.12611088846485785 1105 32-33 0.119936123131275 1105 34-35 0.30780222266955803 1105 36-37 0.2495746398057861 1105 38-39 0.17961823723484827 1105 40-41 0.08503468904861933 1105 42-43 -0.005756105294118186 1105 44-45 -0.10886358437640808 1105 46-47 -0.06853764853414646 1105 48-49 -0.21485787727879568 1105 50-51 -0.23669278492056378 1105 52-53 -0.12894175703110022 1105 54-55 -0.33606008881240257 1105 56-57 -0.20616042090308895 1105 58-59 -0.23808768678193104 1105 60-61 -0.3754024015076993 1105 62-63 -0.28060590860742707 1105 64-65 -0.24543295694290634 1105 66-67 0.027998353802743736 1105 68-69 -0.086977013624292 1105 70-71 -0.06270883494917712 1105 72-73 -0.3527537236647973 1105 74-75 -0.10860050130373722 1105 76-77 -0.1500644212865616 1105 78-79 0.09583675541092518 1105 80-81 0.1518332893085379 1105 82-83 0.1318607773784457 1105 84-85 0.06683150178196229 1105 86-87 0.1764504354545231 1105 88-89 0.2805132871382092 1105 90-91 0.3427249329257087 1105 92-93 0.24340872621794318 1105 94-95 0.2742089744991958 1105 96-97 0.08038143272955267 1105 98-99 0.13735893585697667 1105 100-101 0.9012238996911144 1106 1 0.0300346708663497 1106 2 -0.097509868788066 1106 3 -0.0646150239502461 1106 4 -0.12496141289014417 1106 5 -0.14229279740380463 1106 6 -0.13091122029759816 1106 7 -0.030754901597603634 1106 8 -0.04029278732371466 1106 9 -0.13545518365308595 1106 10-11 -0.03589088474792135 1106 12-13 0.01592792677576682 1106 14-15 -0.052235041642767044 1106 16-17 -0.10729844893530327 1106 18-19 -0.05962895379020239 1106 20-21 0.007876990809165818 1106 22-23 0.04379039439410093 1106 24-25 0.032143657546065185 1106 26-27 -0.08217656936247408 1106 28-29 0.06433135679236557 1106 30-31 0.09400502235560992 1106 32-33 0.08847519561660278 1106 34-35 -0.023560494643227514 1106 36-37 -0.03689564920364319 1106 38-39 0.020914184434310812 1106 40-41 -0.02705692108573743 1106 42-43 0.033684534707248304 1106 44-45 -0.08634149509596512 1106 46-47 -0.03542670413459348 1106 48-49 0.001313570589651647 1106 50-51 0.06411736909526411 1106 52-53 0.2543793935350749 1106 54-55 0.17953064715496936 1106 56-57 0.19053345394355858 1106 58-59 0.22813024796118242 1106 60-61 0.30858035880810775 1106 62-63 0.2547786179958891 1106 64-65 0.24023252136962725 1106 66-67 0.22488255766959497 1106 68-69 0.3497292363489919 1106 70-71 0.44096897146879144 1106 72-73 0.6145483704887553 1106 74-75 0.6265354541030668 1106 76-77 0.3589711310202546 1106 78-79 0.6261136034512127 1106 80-81 0.6133710110028439 1106 82-83 0.8863919118291754 1106 84-85 0.6656647346609006 1106 86-87 0.47031160320481646 1106 88-89 0.5223631227777155 1106 90-91 0.7295368737473815 1106 92-93 0.68936907024219 1106 94-95 0.7163098392619283 1106 96-97 0.8126368049024038 1106 98-99 0.7704107831491953 1106 100-101 1.1628862275828133 1107 1 -0.1570745499063122 1107 2 -0.11614037725305337 1107 3 -0.08787382181800041 1107 4 -0.04769082408930814 1107 5 -0.09605231934752823 1107 6 -0.00786496179275531 1107 7 -0.13964412274493299 1107 8 -0.006461455140559735 1107 9 0.0031828324388243345 1107 10-11 0.03138650121232445 1107 12-13 0.11322291889877789 1107 14-15 -0.022860567644990226 1107 16-17 0.008551763303607629 1107 18-19 0.007664868358922661 1107 20-21 0.0782505659418149 1107 22-23 0.016274446872557746 1107 24-25 -0.061500910046085266 1107 26-27 0.11714208875171295 1107 28-29 0.07563804087785542 1107 30-31 0.0010486538979606053 1107 32-33 0.07413665324852303 1107 34-35 -0.05443790789107794 1107 36-37 0.06622411617947677 1107 38-39 0.07516509540566574 1107 40-41 0.038576139053397185 1107 42-43 -0.12301084403733142 1107 44-45 -0.23576182782804267 1107 46-47 -0.29047164208886045 1107 48-49 -0.23831157119457913 1107 50-51 -0.19509352417448866 1107 52-53 -0.17769513980971396 1107 54-55 -0.2942552221762682 1107 56-57 -0.2859635028872418 1107 58-59 -0.303410621771242 1107 60-61 -0.28554085826163345 1107 62-63 -0.2802617461028092 1107 64-65 -0.42838457304443267 1107 66-67 -0.3788773354138826 1107 68-69 -0.5119741961281363 1107 70-71 -0.415833295523484 1107 72-73 -0.242676288884482 1107 74-75 -0.28297926123451944 1107 76-77 -0.5118455496260097 1107 78-79 -0.35685302613682524 1107 80-81 -0.17390600028298664 1107 82-83 -0.2783955505752509 1107 84-85 -0.17951502599284197 1107 86-87 -0.16424405238173634 1107 88-89 -0.09852964151708576 1107 90-91 0.12470226599084455 1107 92-93 0.10996301401911168 1107 94-95 0.024069169626852016 1107 96-97 -0.030107748798513967 1107 98-99 -0.05738461492999747 1107 100-101 0.48321517757898036 1108 1 0.15221878825506963 1108 2 0.061432496036651685 1108 3 0.12570893171897524 1108 4 0.06437440048658516 1108 5 0.06927621755395563 1108 6 0.06404667063215896 1108 7 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46.0 6 87.0 7 185.0 8 469.0 9 866.0 10 1477.0 11 2259.0 12 3201.0 13 4566.0 14 5655.0 15 7963.0 16 10748.0 17 15957.0 18 15636.0 19 10034.0 20 8811.0 21 9601.0 22 10146.0 23 11104.0 24 12887.0 25 15075.0 26 18463.0 27 22263.0 28 27155.0 29 33445.0 30 39926.0 31 49299.0 32 60938.0 33 78804.0 34 106249.0 35 152422.0 36 243181.0 37 392118.0 38 310007.0 39 22764.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.311289481360994 20.53958965020376 16.4108684057766 20.73825246265864 2 15.468146944754857 22.327071340701856 38.46031045460094 23.74447125994234 3 19.21471936068514 26.549690578369596 31.44415150860652 22.79143855233874 4 12.885074984270675 18.565649973236674 38.34850313177887 30.20077191071378 5 13.145382567943114 38.760957028785405 33.81716911753917 14.276491285732313 6 29.098017166092273 37.39412051949028 17.743851477617405 15.764010836800047 7 26.664256402069697 34.547817145432866 21.235115833560275 17.55281061893717 8 26.392397336814135 33.111166411554244 20.093331236090112 20.403105015541513 9 26.569293542055988 17.30466292293101 21.36458930030332 34.76145423470969 10-11 25.04393059377025 26.881503019091173 26.881297598813024 21.193268788325557 12-13 26.963495055827362 24.780728525951037 27.09801599226211 21.157760425959488 14-15 23.552607252590526 25.624880781544835 27.053213249168774 23.769298716695868 16-17 21.29330702711237 29.149458600716816 27.389459727623205 22.16777464454761 18-19 21.317166561804505 27.996553634647707 29.525320690399948 21.160959113147836 20-21 23.153623848472613 26.09811811548611 29.005930189972673 21.74232784606861 22-23 22.513945102311038 26.264567232296294 29.206332344185785 22.015155321206887 24-25 22.758659345096678 26.651667073594453 29.217190273173756 21.372483308135113 26-27 22.289244663768088 26.173360628797344 30.06584013372273 21.47155457371184 28-29 21.50222088666435 27.158644320070618 29.670494135544516 21.668640657720516 30-31 21.991179840171284 26.52782799162355 29.299035581139837 22.18195658706533 32-33 21.95887016499357 26.687146090206497 29.354293635962396 21.999690108837534 34-35 22.78840933568496 26.36215837092121 29.150039470942634 21.699392822451188 36-37 22.628787329623542 26.017166850434396 29.78845042570819 21.56559539423387 38-39 22.225349591085923 26.533556195051943 29.633907070185302 21.607187143676835 40-41 22.0593591024676 26.650790227038957 29.537338245932215 21.752512424561232 42-43 21.913910841259174 26.847104879344297 28.629399318587996 22.609584960808537 44-45 22.314113856610902 26.486095790132573 28.619216189987373 22.58057416326915 46-47 22.84945005927856 26.86692256224249 28.243505769388783 22.040121609090164 48-49 22.697973582295543 26.832273019530533 28.17114723713398 22.298606161039945 50-51 22.00048127290815 27.14373332785543 28.62869501677119 22.22709038246523 52-53 22.497511480058975 27.547886401412352 27.675217628111824 22.279384490416852 54-55 21.903054540844593 26.89726168900074 28.147537539088546 23.05214623106612 56-57 22.666396294452948 27.182590455352194 27.611419958868993 22.539593291325865 58-59 23.21047128469737 26.660451226845066 27.72655468897417 22.402522799483396 60-61 22.520026752225267 26.91280905114998 28.61780854198905 21.949355654635696 62-63 22.425555691198152 26.930305008029 28.507199100376564 22.136940200396285 64-65 22.11728475853362 27.32229773232955 28.170862045792422 22.389555463344404 66-67 22.281530861328125 27.086440360629393 27.569067712799455 23.062961065243023 68-69 21.985378531232612 27.115159326586046 28.10938046573651 22.79008167644483 70-71 22.651870146212286 27.043286161011938 27.65153560461644 22.653308088159342 72-73 22.372351839157094 26.623319075209643 28.213037262064628 22.79129182356863 74-75 21.929964597282346 27.964684145780318 27.439864680859056 22.66548657607828 76-77 22.291653444470303 26.812439912463184 27.805485624828112 23.090421018238406 78-79 22.223294973508096 26.95148702402979 28.024603501831912 22.8006145006302 80-81 22.427844660011832 27.336655663965292 27.62565264956944 22.609847026453437 82-83 22.24364136201861 27.074862192339115 28.07974884729878 22.60174759834349 84-85 21.963917634685274 27.13948154269455 27.76284404962015 23.133756773000027 86-87 22.020594887662114 27.23226881154132 27.742850156586506 23.004286144210063 88-89 22.138925644122157 26.962034308574374 27.425842939394197 23.47319710790927 90-91 21.984971492123712 26.76551399422687 27.93439139666657 23.31512311698285 92-93 22.333850209880833 27.317639615359333 27.650449811717642 22.69806036304219 94-95 21.93190141704777 27.008716862774556 27.67741855966344 23.381963160514232 96-97 21.429688671546117 26.989106482730048 27.9744560944682 23.60674875125564 98-99 21.65646216980158 27.189017175482917 27.228105719839608 23.926414934875904 100-101 21.710115088380764 26.36045687897966 28.16692598576963 23.762502046869947 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 315.0 1 238.5 2 215.0 3 295.5 4 256.5 5 225.5 6 449.5 7 954.5 8 1655.0 9 2196.0 10 2098.0 11 1773.0 12 1835.0 13 1982.0 14 2067.5 15 2226.0 16 2328.5 17 2390.0 18 2499.5 19 2721.0 20 2977.5 21 3250.5 22 3742.5 23 4380.0 24 5011.5 25 6093.0 26 7911.5 27 9809.5 28 11466.0 29 13748.0 30 16787.0 31 20224.5 32 24151.5 33 28648.5 34 33709.0 35 37481.0 36 42548.5 37 48452.0 38 51001.0 39 51994.0 40 52324.0 41 53390.5 42 54677.5 43 56447.0 44 58938.5 45 60652.5 46 61316.0 47 70879.0 48 97984.0 49 96126.0 50 69452.5 51 58304.0 52 52916.5 53 51578.0 54 50198.5 55 46079.5 56 42404.0 57 38829.0 58 34588.5 59 30592.0 60 26215.0 61 21157.0 62 16946.0 63 13449.0 64 10203.5 65 7723.0 66 5515.5 67 4047.5 68 2920.5 69 1923.5 70 1318.5 71 865.5 72 589.5 73 440.0 74 318.0 75 244.0 76 170.0 77 77.0 78 32.5 79 17.5 80 9.5 81 3.5 82 2.5 83 2.0 84 1.5 85 0.5 86 1.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40960803463268514 2 0.0 3 0.0 4 0.0 5 5.282235723877584E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001115138652818601 16-17 0.00261177210791725 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002288968813680286 36-37 0.007835316323751748 38-39 0.013293626571758584 40-41 0.002435697583787997 42-43 5.282235723877584E-4 44-45 3.2280329423696344E-4 46-47 2.3476603217233703E-4 48-49 2.641117861938792E-4 50-51 5.282235723877584E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.4672877010771064E-4 60-61 0.0023770060757449126 62-63 0.0 64-65 2.934575402154213E-4 66-67 0.0014672877010771066 68-69 0.0016433622252063594 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.1255998272122003 78-79 8.80372620646264E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14837213233291702 88-89 0.4426806994149631 90-91 0.33950102827522094 92-93 0.0 94-95 0.0 96-97 7.336438505385533E-4 98-99 0.0 100-101 1.760745241292528E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1703824.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.588932678304644 #Duplication Level Percentage of deduplicated Percentage of total 1 71.85237964298827 32.756732983201786 2 14.893055534970978 13.579170123160885 3 5.354102050409896 7.3226339378672884 4 2.4793614374251858 4.521257666238457 5 1.3443810243711878 3.064444800702416 6 0.8462903719324912 2.3148884875396645 7 0.5891213883223976 1.8800190718105407 8 0.4028994003312027 1.469420291026281 9 0.29917961729372955 1.2275351488372306 >10 1.704074625015147 14.50125705730517 >50 0.15108268466719957 4.774973815559673 >100 0.07684044564774173 6.021027530706203 >500 0.0028410551025227606 0.8897481285235647 >1k 0.003874166048894673 3.743794960692252 >5k 3.874166048894673E-4 1.2541721949273803 >10k+ 1.2913886829648912E-4 0.6789238019012602 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11532 0.6768304707528477 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7682 0.45086816478697334 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6940 0.40731906581900473 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6681 0.3921179652358459 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4203 0.24668040830508314 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3762 0.22079745325808298 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3648 0.21410662134117137 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3629 0.2129914826883528 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3553 0.20853092807707838 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3306 0.1940341255904366 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3020 0.17724835429011446 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3010 0.17666143920968364 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2971 0.17437247039600334 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2967 0.174137704363831 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2584 0.15165885678332972 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2404 0.14109438533557456 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2269 0.1331710317497582 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2255 0.13234935063715503 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1732 0.10165369193062194 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.8691508043084264E-5 0.0 3 0.0 0.0 0.0 5.8691508043084264E-5 0.0 4 0.0 0.0 0.0 1.1738301608616853E-4 0.0 5 0.0 0.0 0.0 1.1738301608616853E-4 0.0 6 5.8691508043084264E-5 0.0 0.0 1.1738301608616853E-4 0.0 7 5.8691508043084264E-5 0.0 0.0 1.1738301608616853E-4 0.0 8 5.8691508043084264E-5 0.0 0.0 1.1738301608616853E-4 0.0 9 5.8691508043084264E-5 0.0 0.0 1.1738301608616853E-4 0.0 10-11 5.8691508043084264E-5 0.0 0.0 2.3476603217233705E-4 0.0 12-13 5.8691508043084264E-5 0.0 0.0 3.521490482585056E-4 2.0542027815079493E-4 14-15 1.1738301608616853E-4 0.0 0.0 4.1084055630158985E-4 3.521490482585056E-4 16-17 1.1738301608616853E-4 0.0 0.0 5.869150804308427E-4 4.695320643446741E-4 18-19 1.4672877010771067E-4 0.0 0.0 7.629896045600954E-4 4.695320643446741E-4 20-21 1.760745241292528E-4 0.0 0.0 7.923353585816376E-4 5.282235723877584E-4 22-23 1.760745241292528E-4 0.0 0.0 9.390641286893482E-4 5.282235723877584E-4 24-25 2.3476603217233705E-4 0.0 0.0 0.0010564471447755167 5.869150804308426E-4 26-27 2.3476603217233705E-4 0.0 0.0 0.0014085961930340222 5.869150804308426E-4 28-29 3.521490482585056E-4 0.0 0.0 0.002465043337809539 5.869150804308426E-4 30-31 3.521490482585056E-4 0.0 0.0 0.006602794654846979 5.869150804308426E-4 32-33 3.521490482585056E-4 0.0 0.0 0.012354562443069237 5.869150804308426E-4 34-35 3.521490482585056E-4 0.0 0.0 0.021803895238005806 6.456065884739269E-4 36-37 4.1084055630158985E-4 0.0 0.0 0.03633004347866916 6.456065884739269E-4 38-39 4.1084055630158985E-4 0.0 0.0 0.05933711463155819 6.74952342495469E-4 40-41 4.1084055630158985E-4 0.0 0.0 0.08900567194733729 7.042980965170112E-4 42-43 4.1084055630158985E-4 0.0 0.0 0.1218142249434214 7.042980965170112E-4 44-45 4.695320643446741E-4 0.0 0.0 0.16345584989998968 7.042980965170112E-4 46-47 5.282235723877584E-4 0.0 0.0 0.21727596277549793 7.042980965170112E-4 48-49 5.282235723877584E-4 0.0 0.0 0.27608485383466835 7.042980965170112E-4 50-51 5.282235723877584E-4 0.0 0.0 0.3429931730037844 7.629896045600954E-4 52-53 5.282235723877584E-4 0.0 0.0 0.4108112105475683 7.629896045600954E-4 54-55 5.282235723877584E-4 0.0 0.0 0.48884157049084886 7.629896045600954E-4 56-57 5.282235723877584E-4 0.0 0.0 0.5870911549549718 7.629896045600954E-4 58-59 5.282235723877584E-4 0.0 0.0 0.6974018443219487 7.629896045600954E-4 60-61 5.282235723877584E-4 0.0 0.0 0.8305728760717069 7.629896045600954E-4 62-63 5.282235723877584E-4 0.0 0.0 0.9660035308811239 7.629896045600954E-4 64-65 5.282235723877584E-4 0.0 0.0 1.1087999699499478 7.629896045600954E-4 66-67 5.282235723877584E-4 0.0 0.0 1.279856370141517 7.629896045600954E-4 68-69 5.282235723877584E-4 0.0 0.0 1.479378151733982 7.629896045600954E-4 70-71 5.869150804308427E-4 0.0 0.0 1.7017015842011851 7.629896045600954E-4 72-73 6.456065884739269E-4 0.0 0.0 1.9354992064908112 8.216811126031797E-4 74-75 6.456065884739269E-4 0.0 0.0 2.1865227863910826 8.803726206462639E-4 76-77 6.456065884739269E-4 0.0 0.0 2.4573547502558952 9.390641286893482E-4 78-79 6.456065884739269E-4 0.0 0.0 2.777282160598747 9.390641286893482E-4 80-81 8.216811126031797E-4 0.0 0.0 3.130839805050287 9.390641286893482E-4 82-83 8.216811126031797E-4 0.0 0.0 3.494169585591 9.390641286893482E-4 84-85 8.216811126031797E-4 0.0 0.0 3.8884297908704184 9.390641286893482E-4 86-87 8.216811126031797E-4 0.0 0.0 4.304024359323498 9.390641286893482E-4 88-89 8.216811126031797E-4 0.0 0.0 4.7531611246232 9.390641286893482E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 505 0.0 31.945446 3 TTGCGTT 640 0.0 25.206951 4 CTTTGCG 645 0.0 25.011549 2 GAGTACT 6785 0.0 24.12842 12-13 GTACTTT 7095 0.0 22.840097 14-15 GCTTATA 925 0.0 22.15069 1 GTACATG 10410 0.0 21.696423 1 AGTACTT 7070 0.0 21.209347 12-13 TACATGG 10605 0.0 21.117996 2 ACCGGTC 135 3.824962E-4 21.08817 8 CGTATAG 205 6.629598E-7 20.9194 1 ACATGGG 10575 0.0 20.684353 3 ACTTTTT 8215 0.0 19.78248 16-17 CATGGGG 4110 0.0 19.625849 4 TACTTTT 8055 0.0 18.232857 14-15 CATGGGA 6430 0.0 17.267374 4 ATGGGGA 2160 0.0 16.914469 5 GTAAGCG 200 4.3837645E-10 16.60742 94-95 CTACACG 430 6.91216E-11 16.55176 4 CCCTATA 375 3.3305696E-9 16.448772 2 >>END_MODULE