FastQCFastQC Report
Thu 26 May 2016
SRR938919_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938919_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1857512
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT106500.5733475746051708No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78630.42330816705356417No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT70260.378247892880369No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55210.29722553609344116No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT49670.26740069512336934No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38600.2078048486362403No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38160.20543608870359922No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34010.18309437570255266No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33270.1791105521794745No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32770.176418779528746No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32350.17415769050213403No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29860.1607526627015061No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA29070.15649966191335507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28470.15326953473248087No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28260.1521389902191749No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG26100.14051053236802777No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT24860.1338349361942211No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA20560.11068569139795598No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA18760.10099530985533338No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG650.005927233529.1472955
GAGTACT66550.022.72813212-13
GTACTTT72700.020.54581814-15
GTACATG99250.020.1323181
AGTACTT68050.020.03573612-13
TACATGG101950.019.2337822
TAAAGCG1250.006132970618.9457424
ACATGGG104050.018.3903883
ACTTTTT87050.017.5140516-17
AGCCCGT2253.6433157E-516.8320396
CATTCCG3101.1613156E-716.7981039
CATGGGG37400.016.5901894
CATGGGA66250.016.0145154
TACTTTT85500.015.808784514-15
CTTTGCG6000.015.7881172
GTATATG8700.015.2749911
AGTACAT82000.014.6136362
GCTTATA10400.014.6035631
AGAGTAC181000.014.55453410-11
GTATCAA307400.014.3280231
Produced by FastQC (version 0.11.5)