##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938919_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1857512 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8923226337165 33.0 31.0 34.0 30.0 34.0 2 31.97735788517113 33.0 31.0 34.0 30.0 34.0 3 32.01374042267291 34.0 31.0 34.0 30.0 34.0 4 35.54254723522647 37.0 35.0 37.0 33.0 37.0 5 35.59025136849721 37.0 35.0 37.0 33.0 37.0 6 35.687895421402395 37.0 35.0 37.0 33.0 37.0 7 35.622401362682986 37.0 35.0 37.0 33.0 37.0 8 35.6175120268402 37.0 35.0 37.0 33.0 37.0 9 37.227142005004545 39.0 37.0 39.0 34.0 39.0 10-11 37.127397831077275 39.0 37.0 39.0 33.0 39.0 12-13 37.0366767482525 39.0 37.0 39.0 33.0 39.0 14-15 38.2338935091671 40.0 38.0 41.0 33.0 41.0 16-17 38.16039142681178 40.0 38.0 41.0 33.0 41.0 18-19 38.12513943382331 40.0 38.0 41.0 33.0 41.0 20-21 38.23613737084875 40.0 38.0 41.0 33.0 41.0 22-23 38.18070811924768 40.0 38.0 41.0 33.0 41.0 24-25 38.104071198463316 40.0 38.0 41.0 33.0 41.0 26-27 37.985913146186945 40.0 38.0 41.0 33.0 41.0 28-29 37.8706934329361 40.0 38.0 41.0 33.0 41.0 30-31 37.69375029609499 40.0 37.5 41.0 32.5 41.0 32-33 37.50269473360065 40.0 37.0 41.0 32.0 41.0 34-35 37.19947488899129 40.0 37.0 41.0 31.0 41.0 36-37 36.94124425575717 40.0 36.0 41.0 30.5 41.0 38-39 36.71543898505097 39.5 36.0 41.0 30.0 41.0 40-41 36.59922816111013 39.0 36.0 41.0 30.0 41.0 42-43 36.393468521333915 39.0 35.5 41.0 30.0 41.0 44-45 36.30180343384053 39.5 36.0 41.0 30.0 41.0 46-47 36.06854706725986 39.5 36.0 41.0 28.5 41.0 48-49 35.81189219773547 39.0 35.0 41.0 27.5 41.0 50-51 34.70375103902424 38.0 34.0 40.0 25.5 40.5 52-53 34.811988563196365 38.5 34.5 39.5 26.0 40.5 54-55 35.32390530989841 39.0 35.0 40.5 26.0 41.0 56-57 35.256248142676874 39.0 35.0 41.0 26.0 41.0 58-59 35.072954575798164 39.0 35.0 41.0 26.0 41.0 60-61 34.751074286464906 38.0 34.5 40.0 25.5 41.0 62-63 34.393427068035095 38.0 34.0 40.0 24.5 41.0 64-65 34.02086366063853 37.5 34.0 40.0 23.0 41.0 66-67 33.603946838566856 37.0 33.5 39.5 22.0 41.0 68-69 33.17124276989866 36.0 33.0 39.0 22.0 41.0 70-71 32.70990416212654 36.0 32.5 39.0 20.5 40.0 72-73 32.268708358277095 35.0 32.0 38.0 19.5 40.0 74-75 31.805841361993892 35.0 31.5 37.0 18.0 39.0 76-77 31.373336753679116 35.0 31.0 37.0 16.0 39.0 78-79 30.97633904922283 35.0 31.0 36.0 11.5 39.0 80-81 30.539361522294335 35.0 31.0 36.0 7.5 37.5 82-83 30.080423706549407 34.0 30.0 35.0 4.5 37.0 84-85 29.67825968284458 34.0 30.0 35.0 2.0 36.5 86-87 29.2755134287154 34.0 29.5 35.0 2.0 36.0 88-89 28.816831600549552 34.0 29.0 35.0 2.0 36.0 90-91 28.374299869933544 34.0 29.0 35.0 2.0 35.0 92-93 27.922718938020317 34.0 28.0 35.0 2.0 35.0 94-95 27.41440782078393 34.0 27.0 35.0 2.0 35.0 96-97 26.8082889908652 33.0 25.5 35.0 2.0 35.0 98-99 26.01840661056295 33.0 24.5 35.0 2.0 35.0 100-101 22.852411182269616 28.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1420140281258071 1101 2 0.20797759095749413 1101 3 0.2993172901849448 1101 4 0.25573929634904147 1101 5 0.07565857113270624 1101 6 0.3156178230104203 1101 7 0.2177355129635714 1101 8 0.25453575379830795 1101 9 0.24446991263803852 1101 10-11 0.19739564050536984 1101 12-13 0.18230384605820404 1101 14-15 0.43864193061390466 1101 16-17 0.3132239996211297 1101 18-19 0.41896518346677425 1101 20-21 0.3511702993963155 1101 22-23 0.48035729825932805 1101 24-25 0.3276593837181565 1101 26-27 0.45899845599503664 1101 28-29 0.33266052122499445 1101 30-31 0.3484726709634529 1101 32-33 0.3769171070018942 1101 34-35 0.3385633102806125 1101 36-37 0.3282276168580225 1101 38-39 0.39118687305765576 1101 40-41 0.27491293072042566 1101 42-43 0.2480031483502998 1101 44-45 0.34101777717093995 1101 46-47 0.35157472731276584 1101 48-49 0.3909474232537704 1101 50-51 0.3589104816284916 1101 52-53 0.3075766351412099 1101 54-55 0.34501878719361656 1101 56-57 0.37153517713283435 1101 58-59 0.3529720098279512 1101 60-61 0.5213670702651427 1101 62-63 0.24883607508602523 1101 64-65 0.22334584759303766 1101 66-67 0.3346435422332803 1101 68-69 0.35230992566179253 1101 70-71 0.2449912391627933 1101 72-73 0.35271234626570447 1101 74-75 0.06747230256044645 1101 76-77 0.04137576550524713 1101 78-79 0.24866022556848577 1101 80-81 0.023999980890209827 1101 82-83 0.018276796020810337 1101 84-85 -0.09537628718127777 1101 86-87 -0.18763111561066026 1101 88-89 -0.2985568016694202 1101 90-91 -0.3271201706375706 1101 92-93 -0.310797735310139 1101 94-95 -0.4549382282295724 1101 96-97 -0.538652910095454 1101 98-99 -0.6204762529810033 1101 100-101 -0.41049830534378984 1102 1 0.34409351444442393 1102 2 0.3029247119915688 1102 3 0.4043841582952936 1102 4 0.21885901544702335 1102 5 0.2490952842433316 1102 6 0.31197714817409405 1102 7 0.45711915126025104 1102 8 0.3935060128772605 1102 9 0.5512987134885776 1102 10-11 0.4785487656802374 1102 12-13 0.39683052335662694 1102 14-15 0.5464919390073888 1102 16-17 0.6771498431665393 1102 18-19 0.5398995883685629 1102 20-21 0.5247196251195589 1102 22-23 0.6091728374009548 1102 24-25 0.6139842955865404 1102 26-27 0.6162381879630274 1102 28-29 0.6286697680494555 1102 30-31 0.6343555258680453 1102 32-33 0.5606344423496736 1102 34-35 0.6182846465236835 1102 36-37 0.45589674561410476 1102 38-39 0.67098165230896 1102 40-41 0.6537295190478858 1102 42-43 0.5531420605544284 1102 44-45 0.6866202896212812 1102 46-47 0.7918463265517559 1102 48-49 0.6511049410199874 1102 50-51 0.6303537416587162 1102 52-53 0.6363904256397888 1102 54-55 0.7686261678662163 1102 56-57 0.5496977729584742 1102 58-59 0.5461428592487962 1102 60-61 0.6182478407872125 1102 62-63 0.6972304710347714 1102 64-65 0.5915705552199171 1102 66-67 0.6163642769431448 1102 68-69 0.6382837100051404 1102 70-71 0.65749963264944 1102 72-73 0.5778078221763963 1102 74-75 0.623614236419769 1102 76-77 0.6949475438052879 1102 78-79 0.5989235712122642 1102 80-81 0.44070756580431336 1102 82-83 0.31722166480263425 1102 84-85 0.3976427800225686 1102 86-87 0.5056827728119195 1102 88-89 0.3429643778952389 1102 90-91 0.42453229704750584 1102 92-93 0.36207416609437004 1102 94-95 0.3610293309445112 1102 96-97 0.30740040253394696 1102 98-99 0.11803795998973499 1102 100-101 0.4452438887136232 1103 1 0.18993104370108682 1103 2 0.177965867628064 1103 3 0.2597622184493318 1103 4 0.19770811787530818 1103 5 0.11170571566460552 1103 6 0.18124962672839473 1103 7 0.21718144608476564 1103 8 0.1712924388873489 1103 9 0.2399347984751401 1103 10-11 0.2408675603401278 1103 12-13 0.23426575919391723 1103 14-15 0.41006840859078153 1103 16-17 0.36913486044846167 1103 18-19 0.4902001106146514 1103 20-21 0.33528797929359655 1103 22-23 0.35724489386029035 1103 24-25 0.31738681630900345 1103 26-27 0.4477060287075929 1103 28-29 0.3152887102497246 1103 30-31 0.36858585691778956 1103 32-33 0.287869348390835 1103 34-35 0.24057302389642388 1103 36-37 0.3095665885545529 1103 38-39 0.32741405443430693 1103 40-41 0.13784034377239607 1103 42-43 0.1297042871880123 1103 44-45 0.30820850457562443 1103 46-47 0.2848023413360963 1103 48-49 0.2463638805752737 1103 50-51 0.14122709526105126 1103 52-53 0.16925914225895156 1103 54-55 0.22060425584766818 1103 56-57 0.21071377647410117 1103 58-59 0.17185180383230403 1103 60-61 0.3178385574532214 1103 62-63 0.3831041345958752 1103 64-65 0.20996206207414048 1103 66-67 0.2576613923979636 1103 68-69 0.44526825201695175 1103 70-71 0.3101708573171962 1103 72-73 0.27538966162326517 1103 74-75 0.0950325939689236 1103 76-77 0.21706206874870304 1103 78-79 0.16323588291231061 1103 80-81 0.2054223417454537 1103 82-83 0.10795782376602148 1103 84-85 0.15073582413452513 1103 86-87 0.2958103793929645 1103 88-89 0.4261473238260294 1103 90-91 0.27674120099197097 1103 92-93 0.30017230041810095 1103 94-95 0.20626278500104434 1103 96-97 0.074998139421659 1103 98-99 0.028882452986731977 1103 100-101 0.052128348483041265 1104 1 0.2901152288824491 1104 2 0.3159798937784437 1104 3 0.32891265164558803 1104 4 0.28622802897508137 1104 5 0.2861518271570489 1104 6 0.2065165400101776 1104 7 0.27006095091076787 1104 8 0.32566310802151577 1104 9 0.3648365135098217 1104 10-11 0.4268871694139946 1104 12-13 0.44083713415755454 1104 14-15 0.520103193875677 1104 16-17 0.4849255170156539 1104 18-19 0.566158106404508 1104 20-21 0.5453063303199102 1104 22-23 0.5074000238124157 1104 24-25 0.5606113123306997 1104 26-27 0.48065165527129494 1104 28-29 0.5816802802512342 1104 30-31 0.5970632211731726 1104 32-33 0.6625625136965283 1104 34-35 0.6415775384243716 1104 36-37 0.5449333492373327 1104 38-39 0.7462664437460305 1104 40-41 0.6740601673352771 1104 42-43 0.6400779489095569 1104 44-45 0.6897193562481689 1104 46-47 0.8089596890694892 1104 48-49 0.835965516846997 1104 50-51 0.7059507398734226 1104 52-53 0.6517316848985999 1104 54-55 0.6441459769296145 1104 56-57 0.696540831380716 1104 58-59 0.6363963251499385 1104 60-61 0.6875572380420962 1104 62-63 0.682552355207747 1104 64-65 0.7374624484648251 1104 66-67 0.7488989291894441 1104 68-69 0.5955443610594386 1104 70-71 0.6243964122855772 1104 72-73 0.6765573376301219 1104 74-75 0.6651211634865106 1104 76-77 0.7134360706290259 1104 78-79 0.7768291744952052 1104 80-81 0.7282175357878131 1104 82-83 0.8392936353990486 1104 84-85 0.7062475100059906 1104 86-87 0.8156308713948484 1104 88-89 0.9289392917593169 1104 90-91 0.8172199272323191 1104 92-93 0.7773504471061763 1104 94-95 0.8382730761540138 1104 96-97 0.8795450297022249 1104 98-99 0.8280299975330223 1104 100-101 1.0948655198063015 1105 1 0.06807075441837895 1105 2 0.14577061554296833 1105 3 0.20354419535524926 1105 4 0.28676620968727917 1105 5 0.28123216420629404 1105 6 0.27924499772250044 1105 7 0.26162385732361315 1105 8 0.309382400861935 1105 9 0.25087255088030247 1105 10-11 0.293478095785936 1105 12-13 0.28129765884919067 1105 14-15 0.33920041301200143 1105 16-17 0.3902348425517843 1105 18-19 0.3567944532535918 1105 20-21 0.44853205713543076 1105 22-23 0.3006270442366272 1105 24-25 0.363444117851941 1105 26-27 0.17566563315750017 1105 28-29 0.28389847352426756 1105 30-31 0.34048136981176924 1105 32-33 0.4571680667339919 1105 34-35 0.5467189910778032 1105 36-37 0.42401500556993454 1105 38-39 0.615173028411732 1105 40-41 0.6734513812635896 1105 42-43 0.5722118798703519 1105 44-45 0.48117235529721825 1105 46-47 0.6587272838756988 1105 48-49 0.523682455517104 1105 50-51 0.40934848701933646 1105 52-53 0.42419480470749704 1105 54-55 0.5579353517611239 1105 56-57 0.5546413398380565 1105 58-59 0.5262164147940567 1105 60-61 0.6630710031247347 1105 62-63 0.5987773680697686 1105 64-65 0.6466545750291246 1105 66-67 0.8058275861767612 1105 68-69 0.810615692222278 1105 70-71 0.7065968505325557 1105 72-73 0.5119140329437997 1105 74-75 0.5989239483988911 1105 76-77 0.5476043246819202 1105 78-79 0.4772382905852268 1105 80-81 0.41338626284724356 1105 82-83 0.4086034112294996 1105 84-85 0.44874433276481085 1105 86-87 0.5464220167915279 1105 88-89 0.5371146023982121 1105 90-91 0.6310523565208044 1105 92-93 0.8226351447029288 1105 94-95 0.6356875375141193 1105 96-97 0.4854338129299478 1105 98-99 0.3918848921631657 1105 100-101 0.6910571753390506 1106 1 0.24491085352263298 1106 2 0.152422035401937 1106 3 0.2109045917722483 1106 4 0.159667867777614 1106 5 0.23633317430730472 1106 6 0.20224480713740434 1106 7 0.10424344947150388 1106 8 0.19330559506814637 1106 9 0.27903340470152926 1106 10-11 0.3655702436799757 1106 12-13 0.3421252746296375 1106 14-15 0.4129673274392971 1106 16-17 0.497648602795735 1106 18-19 0.499183437435029 1106 20-21 0.3518449025709174 1106 22-23 0.4202382506402813 1106 24-25 0.38988160594037424 1106 26-27 0.3147722655188474 1106 28-29 0.31387083868531107 1106 30-31 0.33998060747138936 1106 32-33 0.3993774244622088 1106 34-35 0.3378887071060106 1106 36-37 0.304418093048497 1106 38-39 0.33551226988304705 1106 40-41 0.35671848627598024 1106 42-43 0.5244317197788675 1106 44-45 0.526216189869352 1106 46-47 0.3764755209635595 1106 48-49 0.5523759946823432 1106 50-51 0.42339460861261813 1106 52-53 0.45684250815708793 1106 54-55 0.46978069195552763 1106 56-57 0.5230232723709278 1106 58-59 0.6229521685581076 1106 60-61 0.6714662766143462 1106 62-63 0.6181813131812604 1106 64-65 0.4661037841009801 1106 66-67 0.4998617962015288 1106 68-69 0.43635754470940924 1106 70-71 0.5659436201151706 1106 72-73 0.3728512351545916 1106 74-75 0.4857659533541039 1106 76-77 0.2790146543941354 1106 78-79 0.3797911779494356 1106 80-81 0.5390595046997291 1106 82-83 0.5736934626874763 1106 84-85 0.7882149826599907 1106 86-87 0.6120514240718755 1106 88-89 0.645490817378203 1106 90-91 0.6277409404735401 1106 92-93 0.5259284551660492 1106 94-95 0.4630974860561423 1106 96-97 0.13255740736486388 1106 98-99 0.25876977876503204 1106 100-101 0.10229931370382772 1107 1 0.16661797476282914 1107 2 0.125264972836316 1107 3 0.18268768114288747 1107 4 0.18340976142223298 1107 5 0.2290146022611097 1107 6 0.2655552882688994 1107 7 0.1635711623984406 1107 8 0.2749197634332887 1107 9 0.280992517789052 1107 10-11 0.21743686711274535 1107 12-13 0.41099918606191466 1107 14-15 0.4141769500691552 1107 16-17 0.15756460551329354 1107 18-19 0.2282863341060164 1107 20-21 0.29632501497904684 1107 22-23 0.30719396677887545 1107 24-25 0.3923967378037503 1107 26-27 0.2137869959246288 1107 28-29 0.2186608454792136 1107 30-31 0.2824799898443118 1107 32-33 0.3379116410022718 1107 34-35 0.3382019984407023 1107 36-37 0.4501981560464614 1107 38-39 0.4923055501004612 1107 40-41 0.20078725905097627 1107 42-43 0.3859895296732603 1107 44-45 0.2759978586977141 1107 46-47 0.4612247643703924 1107 48-49 0.4639370471188826 1107 50-51 0.37178569007763684 1107 52-53 0.4578441448688437 1107 54-55 0.4738426708326742 1107 56-57 0.37616390605631267 1107 58-59 0.4212912149419026 1107 60-61 0.47465014419510254 1107 62-63 0.5086876129774467 1107 64-65 0.43358880664436583 1107 66-67 0.4660209278178655 1107 68-69 0.6290307891044407 1107 70-71 0.4646618895241019 1107 72-73 0.5241304952373511 1107 74-75 0.4117260546449444 1107 76-77 0.5367424043786961 1107 78-79 0.5061678687035567 1107 80-81 0.5446584485574277 1107 82-83 0.6543674482350035 1107 84-85 0.5625042483013942 1107 86-87 0.6055850229848545 1107 88-89 0.6410161092069941 1107 90-91 0.8478555102962808 1107 92-93 0.8471847086402029 1107 94-95 0.9624638006753727 1107 96-97 0.9413093374494785 1107 98-99 0.7622664744601728 1107 100-101 0.7023247908207431 1108 1 0.1097906047758812 1108 2 0.16274629455543632 1108 3 0.13589094877459473 1108 4 0.10990868255012742 1108 5 0.05064531340242695 1108 6 0.11251449430029936 1108 7 0.1064120515401612 1108 8 0.1272693618046148 1108 9 0.3262622897830738 1108 10-11 0.2498619182786186 1108 12-13 0.2009511836340181 1108 14-15 0.29596529685360196 1108 16-17 0.3424547453174682 1108 18-19 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-1.8062771306737275 2316 64-65 -1.8776480579267272 2316 66-67 -1.9502126217177107 2316 68-69 -1.9834280432658318 2316 70-71 -1.7423698193189594 2316 72-73 -1.9124899083762052 2316 74-75 -1.763184827665647 2316 76-77 -1.8265674263603593 2316 78-79 -1.6902662933149344 2316 80-81 -1.6293732981296216 2316 82-83 -1.3919188273213656 2316 84-85 -1.5774930927894637 2316 86-87 -1.6227091123265893 2316 88-89 -1.892705469093947 2316 90-91 -2.0256999672777134 2316 92-93 -2.116768273392747 2316 94-95 -2.1718804096114113 2316 96-97 -2.3028809952287084 2316 98-99 -2.609371983687076 2316 100-101 -2.2332334007081975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5253.0 3 1262.0 4 1254.0 5 1362.0 6 1678.0 7 2013.0 8 2388.0 9 2975.0 10 3862.0 11 5018.0 12 7111.0 13 10630.0 14 9712.0 15 10119.0 16 13455.0 17 19100.0 18 15947.0 19 10497.0 20 10018.0 21 10614.0 22 11631.0 23 13134.0 24 14861.0 25 17406.0 26 20423.0 27 23908.0 28 28374.0 29 34536.0 30 41341.0 31 50944.0 32 63611.0 33 81661.0 34 110600.0 35 156340.0 36 241938.0 37 386635.0 38 365746.0 39 50150.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.47439775305175 20.34255158258615 16.134222750351086 21.048827914011017 2 14.914812220486018 22.000215528853925 39.493345546635055 23.591626704025 3 19.30497124254928 26.408355261349804 31.117216048128647 23.169457447972267 4 12.676933788971242 18.287348287612357 39.02289704440745 30.01282087900895 5 13.258033689727453 38.2983092614142 34.040336476867076 14.403320571991271 6 29.014558828915987 37.30217811457025 18.3354628251066 15.347800231407167 7 26.14059403487244 34.60692956292828 21.767722119366116 17.48475428283317 8 25.560799240255268 33.0317660994617 20.86389181717646 20.54354284310657 9 26.32113388650038 17.674123450379 21.915477813612906 34.089264849507714 10-11 24.288152182637265 27.088516974511435 27.54631138423218 21.077019458619123 12-13 26.483212846992448 25.024071681494824 27.47077798866591 21.021937482846823 14-15 23.389189505104998 26.02271683622283 27.46704825220197 23.121045406470202 16-17 21.12014216503253 29.298294472090042 27.821834764931047 21.759728597946385 18-19 20.97878043958305 28.4740706981084 29.918696690494162 20.628452171814384 20-21 22.88522491795931 26.690517704169736 29.215401966390726 21.20885541148023 22-23 22.147285228237386 26.73433985504299 29.38985559526911 21.728519321450516 24-25 22.27765560699242 27.074288399331603 29.369602339931273 21.2784536537447 26-27 22.080637248232552 26.635439362299145 30.112247484918676 21.17167590454963 28-29 21.252557385935333 27.726643529631733 29.711261227038754 21.30953785739418 30-31 21.812625925066857 27.074357447099683 29.430562099595665 21.68245452823779 32-33 21.67238629874258 27.15973127606159 29.43822797358015 21.729654451615684 34-35 22.396766014308156 26.96845167992961 29.14864824356326 21.48613406219897 36-37 22.330363696425533 26.628144084701344 29.70417892817652 21.337313290696603 38-39 21.980500521066194 27.072424820006997 29.567402812973075 21.379671845953737 40-41 21.96987192349483 27.174274583782456 29.454919028022587 21.400934464700132 42-43 21.612152345245196 27.319269571662563 28.730406696720213 22.33817138637203 44-45 22.06470969373883 26.926877964914087 28.75329885792896 22.255113483418125 46-47 22.470505552627962 27.28840986975157 28.352081951443314 21.889002626177152 48-49 22.290561955716502 27.210048718666073 28.41657029898423 22.082819026633196 50-51 21.77031978221239 27.503018064012718 28.819061718935586 21.90760043483931 52-53 22.15358833538369 27.925562640198414 27.78893217474306 22.13191684967483 54-55 21.585036433654498 27.16515462829827 28.46911018559724 22.78069875244999 56-57 22.3579556749707 27.51896732705322 27.75178719268954 22.371289805286537 58-59 22.787550612515208 27.107215378037935 27.992219470847218 22.11301453859964 60-61 22.331238162120464 27.16672897307417 28.649729595597627 21.85230326920774 62-63 22.162253842077515 27.207316238557894 28.528634375533407 22.101795543831184 64-65 21.837666050045605 27.54970374320641 28.326256666420452 22.28637354032753 66-67 22.00805738451064 27.257101354839897 27.81938101881969 22.915460241829773 68-69 21.737621963084354 27.22089658309863 28.33464995143533 22.706831502381686 70-71 22.296196325061832 27.311301313050095 27.79499109234651 22.597511269541563 72-73 22.051039211600852 26.757946633690427 28.524755181175397 22.666258973533317 74-75 21.710230436893283 27.937287004580163 27.74897699960655 22.603505558920006 76-77 22.016417209096094 27.005830130066737 27.92086107701718 23.05689158381999 78-79 21.88196881110071 27.082850432899676 28.29785271701309 22.73732803898653 80-81 22.22560331552714 27.367948696101653 27.811737478932407 22.594710509438794 82-83 21.896500699816755 27.258092527298807 28.239080342858813 22.60632643002563 84-85 21.842836301407477 27.199030897709985 27.950204200218742 23.007928600663796 86-87 21.836814634829896 27.39726657371654 28.00265973706692 22.763259054386644 88-89 22.03306469284897 26.8944835366398 27.80999840384808 23.26245336666315 90-91 21.884600294925388 26.65543785597359 28.25994020320089 23.200021645900133 92-93 22.225607561705928 27.159511363827004 28.149696104147715 22.465184970319353 94-95 21.960105353085186 26.909524669026187 28.019369034278718 23.111000943609906 96-97 21.46736571633289 26.866285104462396 28.303610373614198 23.36273880559052 98-99 21.709214281191468 26.977366808585252 27.673123267847295 23.640295642375985 100-101 22.034435704399638 26.136674750216155 28.462534858278172 23.366354687106035 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1286.0 1 733.0 2 175.5 3 179.0 4 204.5 5 233.5 6 372.0 7 700.0 8 1191.5 9 1583.0 10 1674.5 11 1748.0 12 1911.0 13 2090.0 14 2340.5 15 2673.5 16 2960.5 17 3053.0 18 3058.5 19 3138.0 20 3349.5 21 3740.5 22 4219.0 23 4849.0 24 5580.5 25 6811.5 26 9066.5 27 11586.0 28 13680.5 29 16287.5 30 19572.0 31 23446.0 32 27966.5 33 33083.0 34 39075.5 35 44033.5 36 49658.5 37 55935.0 38 58261.5 39 58191.5 40 58814.0 41 60136.0 42 61805.0 43 64733.0 44 67537.0 45 68395.0 46 69986.0 47 81181.5 48 108819.0 49 107986.5 50 79850.0 51 66244.0 52 59346.5 53 55669.5 54 51657.5 55 45920.5 56 40685.0 57 35626.0 58 31280.0 59 26998.0 60 22577.0 61 18407.0 62 14559.0 63 11057.0 64 7935.0 65 5797.5 66 4197.5 67 3139.5 68 2317.0 69 1598.5 70 1137.5 71 825.5 72 597.5 73 466.5 74 359.0 75 260.5 76 187.5 77 109.0 78 60.5 79 43.0 80 39.5 81 29.5 82 16.5 83 11.0 84 8.0 85 8.0 86 7.0 87 4.5 88 4.0 89 6.0 90 5.0 91 2.0 92 3.0 93 3.0 94 1.5 95 1.5 96 1.0 97 1.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.329042288825052 2 0.08678275025948688 3 0.09792668903350286 4 0.1047099561133387 5 0.1167690975886024 6 0.05620421294721111 7 0.05862680833286676 8 0.05916516286301246 9 0.0630413154800615 10-11 0.0598111882991873 12-13 0.0580077006231992 14-15 0.05639263703276211 16-17 0.05803461834970649 18-19 0.06745582262725625 20-21 0.06829027214898208 22-23 0.08519460439555707 24-25 0.09318916916822072 26-27 0.08923226337164981 28-29 0.08683658571250145 30-31 0.08061859088931861 32-33 0.07170882341540727 34-35 0.325731408464656 36-37 0.5070492142177278 38-39 0.4790547786501514 40-41 0.4597009332914135 42-43 0.49229830009173564 44-45 0.502527036164504 46-47 0.4951784968280151 48-49 0.5143977535542166 50-51 0.5099832464070219 52-53 0.5093641386973543 54-55 0.5001044407788482 56-57 0.47765505687177257 58-59 0.45962018011189165 60-61 0.4189744130858913 62-63 0.49154460374953163 64-65 0.5134556331264617 66-67 0.5257570341402908 68-69 0.5370355615468434 70-71 0.5353128270503771 72-73 0.523119096942577 74-75 0.525487856875218 76-77 0.4989738962655423 78-79 0.4777088923247871 80-81 0.4843844884985938 82-83 0.4937249395966218 84-85 0.49547459181959524 86-87 0.49577068681117537 88-89 0.5015849157367489 90-91 0.5161204880506829 92-93 0.520858007915965 94-95 0.528691066329585 96-97 0.5205888306508921 98-99 0.5243034769088974 100-101 0.5014234093777052 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1857512.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.2817469841737 #Duplication Level Percentage of deduplicated Percentage of total 1 73.76875432735696 37.829905947531884 2 14.458230547767991 14.828866415789788 3 5.034010209767099 7.7445851367907075 4 2.2485808632036597 4.612446196010598 5 1.2643312596111742 3.2418557879780936 6 0.7272057758003474 2.23754295600139 7 0.4862254672307194 1.745414397145118 8 0.33318746738620836 1.366914832063773 9 0.2620562954119414 1.2094834173232556 >10 1.2691705652182301 11.744844310503598 >50 0.09926416780112118 3.5146827733060673 >100 0.043190140402037294 3.889735841859133 >500 0.002211839525335839 0.7657942541922587 >1k 0.0031597707504797696 3.5902884659623093 >5k 3.1597707504797695E-4 1.1024023647950345 >10k+ 1.05325691682659E-4 0.5752369027470415 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10650 0.5733475746051708 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7863 0.42330816705356417 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7026 0.378247892880369 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5521 0.29722553609344116 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4967 0.26740069512336934 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3860 0.2078048486362403 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3816 0.20543608870359922 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3401 0.18309437570255266 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3327 0.1791105521794745 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3277 0.176418779528746 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3235 0.17415769050213403 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2986 0.1607526627015061 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2907 0.15649966191335507 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2847 0.15326953473248087 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2826 0.1521389902191749 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2610 0.14051053236802777 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2486 0.1338349361942211 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2056 0.11068569139795598 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1876 0.10099530985533338 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.3835453014570025E-5 0.0 14-15 0.0 0.0 0.0 1.3458863253642506E-4 0.0 16-17 0.0 0.0 0.0 2.9609499158013517E-4 0.0 18-19 0.0 0.0 0.0 3.2301271808742016E-4 0.0 20-21 0.0 0.0 0.0 3.7684817110199016E-4 0.0 22-23 0.0 0.0 0.0 5.383545301457003E-4 0.0 24-25 0.0 0.0 0.0 7.267786156966954E-4 0.0 26-27 0.0 0.0 0.0 0.0013189685988569655 0.0 28-29 0.0 0.0 0.0 0.0036069753519761922 0.0 30-31 0.0 0.0 0.0 0.0104171601583193 0.0 32-33 0.0 0.0 0.0 0.02215328891549557 0.0 34-35 0.0 0.0 0.0 0.04126487473566792 0.0 36-37 0.0 0.0 0.0 0.06850561396104035 0.0 38-39 0.0 0.0 0.0 0.11267760315949507 0.0 40-41 0.0 0.0 0.0 0.17025462015857773 0.0 42-43 0.0 0.0 0.0 0.23337668881816107 0.0 44-45 0.0 0.0 0.0 0.3157987673834678 0.0 46-47 0.0 0.0 0.0 0.4104414937830819 0.0 48-49 0.0 0.0 0.0 0.5118944049890392 0.0 50-51 0.0 0.0 0.0 0.6268600149016534 0.0 52-53 0.0 0.0 0.0 0.7456479419783022 0.0 54-55 0.0 0.0 0.0 0.8793482895399869 0.0 56-57 0.0 0.0 0.0 1.0430080667042798 0.0 58-59 0.0 0.0 0.0 1.216250554505166 0.0 60-61 0.0 0.0 0.0 1.4154955661120896 0.0 62-63 0.0 0.0 0.0 1.6354403094031156 0.0 64-65 0.0 0.0 0.0 1.8618183893293825 0.0 66-67 0.0 0.0 0.0 2.1294613439913173 0.0 68-69 0.0 0.0 0.0 2.43212426083923 0.0 70-71 0.0 0.0 0.0 2.766657765871768 0.0 72-73 0.0 0.0 0.0 3.11919922993768 0.0 74-75 0.0 0.0 0.0 3.507433599352252 0.0 76-77 0.0 0.0 0.0 3.9170944790666224 0.0 78-79 0.0 0.0 0.0 4.39569165636615 0.0 80-81 0.0 0.0 0.0 4.9113007076131945 0.0 82-83 0.0 0.0 0.0 5.438349792625835 0.0 84-85 0.0 0.0 0.0 6.024106439150865 0.0 86-87 0.0 0.0 0.0 6.630697405992532 0.0 88-89 0.0 0.0 0.0 7.277315032150533 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATACCG 65 0.0059272335 29.147295 5 GAGTACT 6655 0.0 22.728132 12-13 GTACTTT 7270 0.0 20.545818 14-15 GTACATG 9925 0.0 20.132318 1 AGTACTT 6805 0.0 20.035736 12-13 TACATGG 10195 0.0 19.233782 2 TAAAGCG 125 0.0061329706 18.945742 4 ACATGGG 10405 0.0 18.390388 3 ACTTTTT 8705 0.0 17.51405 16-17 AGCCCGT 225 3.6433157E-5 16.832039 6 CATTCCG 310 1.1613156E-7 16.798103 9 CATGGGG 3740 0.0 16.590189 4 CATGGGA 6625 0.0 16.014515 4 TACTTTT 8550 0.0 15.8087845 14-15 CTTTGCG 600 0.0 15.788117 2 GTATATG 870 0.0 15.274991 1 AGTACAT 8200 0.0 14.613636 2 GCTTATA 1040 0.0 14.603563 1 AGAGTAC 18100 0.0 14.554534 10-11 GTATCAA 30740 0.0 14.328023 1 >>END_MODULE