FastQCFastQC Report
Thu 26 May 2016
SRR938919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938919_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1857512
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT108230.5826611079766915No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74620.40172015039472153No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT66930.3603206870265172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59340.31945957818845855No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48620.26174797255683946No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40110.21593400204144036No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38370.20656663321690522No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC37140.19994487249611306No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35860.1930539345102481No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32640.17571891863955655No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31200.1679666134054585No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30210.16263690355701604No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30180.16247539719797233No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG29030.15628432010129678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29020.15623048464828224No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28940.15579980102416566No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT23870.12850522634577866No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA23360.12575961824203558No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA19060.10261037344577047No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT68800.024.62695912-13
GTACTTT74850.022.47788414-15
AGTACTT72300.020.41517612-13
CTTTGCG5850.020.2794742
TCGCGCT858.0178106E-519.54039660-61
GTACATG98750.019.493311
ACTTTTT87050.019.32606516-17
TACATGG101700.018.5243132
TATCCCG1300.007607248618.2515284
TTGCGTT7050.018.173864
ACATGGG103150.017.4358273
TTTGCGT6850.017.3189683
CATGGGG37300.016.9205844
TACTTTT86750.016.79576914-15
GTAAGCG2351.4551915E-1116.15454394-95
CTCCGAC3850.016.02341894-95
AGAGTAC189600.014.84387910-11
CGCGGAA1157.692179E-414.52953382-83
GACATAT6300.014.3699231
TAATACC5953.6379788E-1214.3558234
Produced by FastQC (version 0.11.5)