##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938919_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1857512 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17509227396647 33.0 31.0 34.0 30.0 34.0 2 32.41939540632846 34.0 31.0 34.0 31.0 34.0 3 32.49853782909612 34.0 31.0 34.0 30.0 34.0 4 36.04797707901753 37.0 35.0 37.0 35.0 37.0 5 35.97364323891313 37.0 35.0 37.0 35.0 37.0 6 36.02122839583271 37.0 35.0 37.0 35.0 37.0 7 35.963792966075054 37.0 35.0 37.0 35.0 37.0 8 35.93987548936427 37.0 35.0 37.0 35.0 37.0 9 37.605395819784746 39.0 37.0 39.0 35.0 39.0 10-11 37.52015114841788 39.0 37.0 39.0 35.0 39.0 12-13 37.43781009220936 39.0 37.0 39.0 34.0 39.0 14-15 38.714963079646324 40.0 38.0 41.0 34.0 41.0 16-17 38.81003433625193 40.0 38.0 41.0 35.0 41.0 18-19 38.882731040230155 40.0 38.0 41.0 35.0 41.0 20-21 38.868386045419896 40.0 38.0 41.0 34.5 41.0 22-23 38.797534820771006 40.0 38.0 41.0 34.0 41.0 24-25 38.71300750681557 40.0 38.0 41.0 34.0 41.0 26-27 38.54431788327612 40.0 38.0 41.0 34.0 41.0 28-29 38.396971055907045 40.0 38.0 41.0 34.0 41.0 30-31 38.21328583610766 40.0 38.0 41.0 34.0 41.0 32-33 38.106061764338534 40.0 37.5 41.0 33.0 41.0 34-35 38.01729894611717 40.0 37.0 41.0 33.0 41.0 36-37 37.88353022753016 40.0 37.0 41.0 33.0 41.0 38-39 37.73643454254939 40.0 37.0 41.0 33.0 41.0 40-41 37.58878354487077 40.0 37.0 41.0 33.0 41.0 42-43 37.39815678175969 40.0 37.0 41.0 32.0 41.0 44-45 37.14134471271249 40.0 36.5 41.0 31.0 41.0 46-47 36.79994261140709 40.0 36.0 41.0 31.0 41.0 48-49 36.43961331070808 39.5 36.0 41.0 30.0 41.0 50-51 36.181729646968634 39.0 35.5 41.0 29.5 41.0 52-53 35.9317029984194 39.0 35.0 40.5 28.5 41.0 54-55 35.65193872233396 39.0 35.0 40.0 28.0 41.0 56-57 35.529607614917154 39.0 35.0 40.0 28.0 41.0 58-59 35.268930967875306 38.0 35.0 40.0 27.5 41.0 60-61 34.90025501854093 38.0 34.0 40.0 26.5 41.0 62-63 34.48582431768946 37.5 34.0 40.0 26.0 41.0 64-65 34.07974107300518 37.0 33.5 39.5 26.0 41.0 66-67 33.6215327276486 36.5 33.0 39.0 25.0 40.5 68-69 33.12860589864292 36.0 32.5 39.0 23.5 40.0 70-71 32.58303041918437 35.0 32.0 38.0 22.0 40.0 72-73 32.058754667533776 35.0 31.0 37.5 21.5 39.5 74-75 31.532238822683247 35.0 31.0 37.0 20.0 39.0 76-77 29.611255001313587 32.5 28.5 35.0 17.5 37.0 78-79 30.573692390681728 34.0 30.0 36.0 18.0 37.5 80-81 30.553445145980213 34.0 30.5 35.5 17.5 37.0 82-83 30.13754392972966 34.0 30.0 35.0 12.5 37.0 84-85 29.68772449383907 34.0 30.0 35.0 7.0 36.0 86-87 29.023792309282527 34.0 29.0 35.0 2.0 36.0 88-89 28.33361937904035 33.0 28.5 35.0 2.0 35.0 90-91 27.758029826994388 33.0 26.5 35.0 2.0 35.0 92-93 27.369787382261862 33.0 26.0 34.5 2.0 35.0 94-95 26.820608157578526 32.5 25.5 34.0 2.0 35.0 96-97 25.971732349508375 32.0 24.5 34.0 2.0 35.0 98-99 24.76490057668537 32.0 11.0 34.0 2.0 35.0 100-101 19.1334158271925 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05907601308067001 1101 2 0.11709650656828074 1101 3 0.13876361352856037 1101 4 0.12304228589227506 1101 5 0.04819429263092445 1101 6 0.06692628374410248 1101 7 0.13763996353601016 1101 8 0.15858009531842043 1101 9 0.17607052800233447 1101 10-11 0.16913360065139926 1101 12-13 0.19670101618603297 1101 14-15 0.2252819080462558 1101 16-17 0.2051047435804989 1101 18-19 0.20417047640024322 1101 20-21 0.25513990650264873 1101 22-23 0.1999936625250669 1101 24-25 0.18323656430217738 1101 26-27 0.1919850996910668 1101 28-29 0.23670532731717486 1101 30-31 0.1620605329126832 1101 32-33 0.33782746996777746 1101 34-35 0.32073250239049145 1101 36-37 0.2155450662447791 1101 38-39 0.2955985116400939 1101 40-41 0.4038532729311868 1101 42-43 0.4304304563296526 1101 44-45 0.5230649912898713 1101 46-47 0.4045958697666734 1101 48-49 0.24483902516342226 1101 50-51 0.16239204160569898 1101 52-53 6.166939789125081E-4 1101 54-55 0.07989354175293073 1101 56-57 0.0673897644240995 1101 58-59 0.10770986001011096 1101 60-61 0.056285304897514266 1101 62-63 0.09917395102500137 1101 64-65 0.20485122895352958 1101 66-67 -0.0820283771454342 1101 68-69 0.1069491830834366 1101 70-71 0.1901375954406248 1101 72-73 0.014891205224749626 1101 74-75 0.2164954489453983 1101 76-77 -0.16075137106303217 1101 78-79 0.28150436888408237 1101 80-81 0.25735095945343645 1101 82-83 0.2893350429841739 1101 84-85 0.4088208243414009 1101 86-87 0.5880669483010088 1101 88-89 0.6441443580023716 1101 90-91 0.571392128581639 1101 92-93 0.37097337384744833 1101 94-95 0.4262492270099685 1101 96-97 0.6579233443252406 1101 98-99 0.6510617060579484 1101 100-101 1.2225971319733162 1102 1 0.18862178556122444 1102 2 0.20046131547656643 1102 3 0.19435787238368363 1102 4 0.15121712221928618 1102 5 0.15584845300247707 1102 6 0.2078116566946946 1102 7 0.1919793961363112 1102 8 0.16245229049496857 1102 9 0.24396544154942035 1102 10-11 0.31933294595943806 1102 12-13 0.25141011192773277 1102 14-15 0.29854515576602125 1102 16-17 0.30309904999872117 1102 18-19 0.29806771214530414 1102 20-21 0.23384668004637632 1102 22-23 0.326119438922575 1102 24-25 0.25779905381032364 1102 26-27 0.3507258668557398 1102 28-29 0.39727237728639153 1102 30-31 0.2421109637783374 1102 32-33 0.33877278640222386 1102 34-35 0.339903925366265 1102 36-37 0.31963339370267363 1102 38-39 0.3298341447887765 1102 40-41 0.409309388985136 1102 42-43 0.6004583646727681 1102 44-45 0.7172608952683888 1102 46-47 0.7406133701863453 1102 48-49 0.7806970493366663 1102 50-51 0.7957113857946041 1102 52-53 0.7033795500026372 1102 54-55 0.75548213500457 1102 56-57 0.7333793565006488 1102 58-59 0.6178502410744002 1102 60-61 0.5965974726553327 1102 62-63 0.7384726052693011 1102 64-65 0.6310801752911672 1102 66-67 0.7074811634510638 1102 68-69 0.8531675676170352 1102 70-71 0.8470828559184866 1102 72-73 0.9850848849293001 1102 74-75 0.8650891902021804 1102 76-77 0.6952657096823103 1102 78-79 0.8766270536806822 1102 80-81 1.0427812655359183 1102 82-83 1.0863717924665757 1102 84-85 1.1127507247386923 1102 86-87 1.2684027577129093 1102 88-89 1.1846324392513239 1102 90-91 1.1531383934800772 1102 92-93 1.2334394501722201 1102 94-95 1.2724116549658788 1102 96-97 1.6124306466586305 1102 98-99 1.5992794890583362 1102 100-101 1.5515560598452858 1103 1 0.03708569878379109 1103 2 0.01960795077081201 1103 3 0.07311715564880217 1103 4 0.050485344006489186 1103 5 0.15199669870472832 1103 6 0.08532283931826612 1103 7 0.12065788348241568 1103 8 0.033229791070112924 1103 9 0.03737516709126254 1103 10-11 -0.006220192683052517 1103 12-13 0.0979316168670934 1103 14-15 0.05841217656632125 1103 16-17 0.11287528731968877 1103 18-19 0.18272585517542694 1103 20-21 0.14005763385149805 1103 22-23 0.14788660340598625 1103 24-25 0.06878860751102422 1103 26-27 0.045565948795072586 1103 28-29 0.03283697751535186 1103 30-31 0.17152956378412654 1103 32-33 0.2435834153705585 1103 34-35 0.11334610703517001 1103 36-37 0.03596808304822474 1103 38-39 -0.02442186462295126 1103 40-41 0.12668829498221612 1103 42-43 0.2020673262006838 1103 44-45 0.29428840445349636 1103 46-47 0.36496159577571774 1103 48-49 0.20534710588692207 1103 50-51 0.22297095077780682 1103 52-53 0.27565298047475295 1103 54-55 0.4009568058627977 1103 56-57 0.4659662172795649 1103 58-59 0.4225806940412582 1103 60-61 0.4912979353893334 1103 62-63 0.4063583282929031 1103 64-65 0.46696045130601505 1103 66-67 0.371035040484216 1103 68-69 0.5318574201179942 1103 70-71 0.5839120190435949 1103 72-73 0.5015695254391161 1103 74-75 0.5955738417327616 1103 76-77 0.2064411008847138 1103 78-79 0.5275442561168227 1103 80-81 0.34985500679335857 1103 82-83 0.45795036425922575 1103 84-85 0.31355028060221457 1103 86-87 0.48242963182694254 1103 88-89 0.5640174948303738 1103 90-91 0.46037847927666675 1103 92-93 0.6548604251509715 1103 94-95 0.6000577040936541 1103 96-97 0.5268313999273175 1103 98-99 0.8608702250106646 1103 100-101 1.016348876516382 1104 1 0.06063966963137801 1104 2 0.013248533873152724 1104 3 0.045929087662941015 1104 4 -0.0021643511666979975 1104 5 -1.6597548322749844E-4 1104 6 0.08607773534374275 1104 7 0.13504106560032625 1104 8 0.0530862224670372 1104 9 0.11954038572089587 1104 10-11 0.1390252447010738 1104 12-13 0.07392005146853364 1104 14-15 0.07030102968454344 1104 16-17 0.1542416997446594 1104 18-19 0.06676916872691407 1104 20-21 0.17258531730945492 1104 22-23 0.008361435368222203 1104 24-25 0.09885925120077843 1104 26-27 0.032634515761465366 1104 28-29 0.17261237148198205 1104 30-31 -0.0029833646117793933 1104 32-33 0.007993088028477757 1104 34-35 0.05021375084843527 1104 36-37 0.08378772600298845 1104 38-39 0.007924772023351068 1104 40-41 0.019656895762011573 1104 42-43 -0.0718831590992508 1104 44-45 0.14822125414043086 1104 46-47 0.1573278995388563 1104 48-49 0.06816896594802557 1104 50-51 0.011984935758178494 1104 52-53 -0.09606683262688875 1104 54-55 0.20774071257290672 1104 56-57 0.09949355585431618 1104 58-59 0.10964001462809136 1104 60-61 0.07199537562701153 1104 62-63 0.05683134472513274 1104 64-65 0.15978070506176323 1104 66-67 0.15110551544441364 1104 68-69 0.03776329197397388 1104 70-71 0.0475821838113788 1104 72-73 0.04795159259213477 1104 74-75 0.26854896897171443 1104 76-77 0.10571225670241091 1104 78-79 0.2261904039198619 1104 80-81 0.26815160095356205 1104 82-83 0.3028307046339833 1104 84-85 0.4549702815336012 1104 86-87 0.40168494731408444 1104 88-89 0.5540310674357123 1104 90-91 0.708018290272566 1104 92-93 0.7237311047463706 1104 94-95 1.049217295930113 1104 96-97 0.5970836371124761 1104 98-99 1.0379068413486792 1104 100-101 1.2736025600512804 1105 1 -0.03485827590078827 1105 2 -0.03898758229883015 1105 3 -0.03910741481416835 1105 4 2.915303584956064E-4 1105 5 -0.040097996840607664 1105 6 -0.023949114442615382 1105 7 -0.02753197144724595 1105 8 -0.04757035800184184 1105 9 -0.00843898763925921 1105 10-11 0.09756311139223328 1105 12-13 0.03539914981340786 1105 14-15 -0.10709271418686228 1105 16-17 -0.09291606422804222 1105 18-19 -0.06333034039302987 1105 20-21 0.05435935843436113 1105 22-23 0.039017492468701676 1105 24-25 -0.0071717302334732835 1105 26-27 -0.21292944331310082 1105 28-29 -0.16642962735080147 1105 30-31 -0.08662800339377696 1105 32-33 -0.01585473619083899 1105 34-35 0.21656787770699992 1105 36-37 0.16499475177936773 1105 38-39 0.09956608494729835 1105 40-41 0.11269352858748505 1105 42-43 0.08569417845657767 1105 44-45 0.09507961188939618 1105 46-47 0.1029617727316321 1105 48-49 0.17903764886676754 1105 50-51 0.18300790373099574 1105 52-53 0.09946705745191764 1105 54-55 0.14576945043544498 1105 56-57 0.105489086485683 1105 58-59 -0.05090373551386307 1105 60-61 0.02646775609411378 1105 62-63 -0.07186000715518759 1105 64-65 -0.28179113811336975 1105 66-67 -0.07774225446308947 1105 68-69 0.013074622109819245 1105 70-71 0.16775694935714824 1105 72-73 0.15093271874268055 1105 74-75 0.22782794506563064 1105 76-77 -0.02963920492727823 1105 78-79 0.04705917571247653 1105 80-81 0.019952037620551977 1105 82-83 -0.07673383310828541 1105 84-85 0.04392710956560464 1105 86-87 -0.04411827999270912 1105 88-89 0.15823914686131246 1105 90-91 -0.031377821593974176 1105 92-93 0.3266179258363415 1105 94-95 0.49865743986629596 1105 96-97 0.607139222786401 1105 98-99 0.7351954967955088 1105 100-101 0.6027306776728985 1106 1 0.11792521942987833 1106 2 0.12650126847304222 1106 3 0.08241440717934978 1106 4 0.04244704779703312 1106 5 0.08474191167854173 1106 6 0.06966437898219624 1106 7 0.17002091591696455 1106 8 0.07627850801683422 1106 9 0.09099116292296827 1106 10-11 0.13036375938155942 1106 12-13 0.1250541907892071 1106 14-15 0.10155174931608713 1106 16-17 0.05683093405669837 1106 18-19 -0.07634539661562911 1106 20-21 0.037540700153442685 1106 22-23 0.05620397998537641 1106 24-25 0.21984370715932755 1106 26-27 0.09529859175455613 1106 28-29 0.08668548604732962 1106 30-31 0.197199421801578 1106 32-33 0.044117152507460844 1106 34-35 -0.04506114840315689 1106 36-37 0.05526728846700735 1106 38-39 0.2490905751321577 1106 40-41 0.4628016856296 1106 42-43 0.31334712299631207 1106 44-45 0.38227134049622435 1106 46-47 0.31763158405239267 1106 48-49 0.3742239457983416 1106 50-51 0.21830474001839661 1106 52-53 0.17327542413764263 1106 54-55 0.22904036714975717 1106 56-57 0.19111992315425397 1106 58-59 0.13844398699423976 1106 60-61 0.28273371759592436 1106 62-63 0.21006680816785206 1106 64-65 0.290684562286863 1106 66-67 0.22515416253710185 1106 68-69 0.2407785481628082 1106 70-71 0.33472092877396165 1106 72-73 0.416339617923164 1106 74-75 0.46040417910412756 1106 76-77 0.1718478352861723 1106 78-79 0.34364722602694187 1106 80-81 0.28223191183439056 1106 82-83 0.4774104398095709 1106 84-85 0.49903907830965366 1106 86-87 0.6636423451264086 1106 88-89 0.8462475326055454 1106 90-91 0.8121460968356082 1106 92-93 0.91004083416491 1106 94-95 0.9012293857401303 1106 96-97 1.1082408046426977 1106 98-99 0.9463394838357253 1106 100-101 1.024025703401886 1107 1 0.05361696175586417 1107 2 0.008413441902725083 1107 3 0.0688497725057644 1107 4 0.009708489338635218 1107 5 0.0330863622992581 1107 6 0.0052415514854402545 1107 7 0.06393234819415738 1107 8 0.06462048812924337 1107 9 -0.04480217905714312 1107 10-11 -0.0040667884536631504 1107 12-13 -0.02442762009338395 1107 14-15 0.01363886561527039 1107 16-17 0.09400945916323167 1107 18-19 0.04945720977062962 1107 20-21 0.12254888371221284 1107 22-23 0.09615472422600391 1107 24-25 0.1525563252805 1107 26-27 0.09371175338755933 1107 28-29 0.07167498638702341 1107 30-31 0.09043625090416896 1107 32-33 0.1773459524580474 1107 34-35 0.08917561893672854 1107 36-37 0.23312659301891614 1107 38-39 0.10068003841423234 1107 40-41 0.08125159607367749 1107 42-43 0.1539539415992408 1107 44-45 0.07769256216257503 1107 46-47 0.08565131723749175 1107 48-49 0.06647257157809605 1107 50-51 0.041510070140070354 1107 52-53 0.2495095974438044 1107 54-55 0.09642703258116825 1107 56-57 0.2557444058915834 1107 58-59 0.15813718074385008 1107 60-61 0.15139485650025364 1107 62-63 0.24883464214969564 1107 64-65 0.3028616977324816 1107 66-67 0.17839871197814716 1107 68-69 0.1479082807302774 1107 70-71 0.2980227631263759 1107 72-73 0.2721070195660502 1107 74-75 0.16026166165616118 1107 76-77 0.20733159169405369 1107 78-79 0.3828222306591442 1107 80-81 0.27550456330743245 1107 82-83 0.27016351250427917 1107 84-85 0.42830271242737084 1107 86-87 0.4761702927516325 1107 88-89 0.8158022234831925 1107 90-91 0.8194396549851994 1107 92-93 0.9835903369751122 1107 94-95 1.0333932422035979 1107 96-97 0.7942490808455283 1107 98-99 0.8786789007957658 1107 100-101 0.5675156051991834 1108 1 0.06033685722415072 1108 2 -0.0491469325549474 1108 3 0.04087465211875241 1108 4 0.016212515149021556 1108 5 -0.08858095144729106 1108 6 0.013161428287354227 1108 7 0.07075573411609071 1108 8 0.09017564616011953 1108 9 0.0656805467671191 1108 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-0.9314440074027672 2316 52-53 -0.7591376321547187 2316 54-55 -0.5700393558092784 2316 56-57 -0.6604995876031836 2316 58-59 -0.7803277489326703 2316 60-61 -0.7297692296484044 2316 62-63 -0.6108527320620354 2316 64-65 -0.6372022635310017 2316 66-67 -0.7347345789895741 2316 68-69 -0.6891470339179477 2316 70-71 -0.6950138192366495 2316 72-73 -0.629551923485618 2316 74-75 -0.8298912821396094 2316 76-77 -0.7529524889107009 2316 78-79 -0.869586637810368 2316 80-81 -0.9185395293680862 2316 82-83 -0.9645450568010965 2316 84-85 -1.2751953749765228 2316 86-87 -1.2100770772137075 2316 88-89 -1.3939145658324144 2316 90-91 -1.4871259338157365 2316 92-93 -1.6128533597637684 2316 94-95 -1.4924060401366752 2316 96-97 -1.3944323459614871 2316 98-99 -1.5212636697157045 2316 100-101 -0.7788522806829938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 15.0 5 49.0 6 86.0 7 222.0 8 557.0 9 998.0 10 1764.0 11 2683.0 12 3854.0 13 5145.0 14 6689.0 15 9263.0 16 12613.0 17 18630.0 18 17721.0 19 11508.0 20 10292.0 21 11057.0 22 11972.0 23 13568.0 24 15546.0 25 18406.0 26 22142.0 27 26720.0 28 32197.0 29 39377.0 30 47727.0 31 58179.0 32 72051.0 33 91917.0 34 124084.0 35 176135.0 36 279132.0 37 427536.0 38 275998.0 39 11677.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.53173784289361 21.000900996825703 16.448407797541094 21.01895336273959 2 15.140521299458632 22.463811808483605 38.79232004961475 23.60334684244301 3 19.279983117201933 26.406612716364684 31.3323951608388 22.98100900559458 4 13.05472050786213 18.703028567244786 38.55022201740823 29.69202890748485 5 13.296710869162624 38.47528306681195 33.904114751344814 14.323891312680617 6 29.18123812928261 37.59889572718777 17.978241863309634 15.241624280219993 7 26.22432587245735 34.61931874464337 21.503118149438606 17.653237233460672 8 25.75407319037508 33.27493981196353 20.57359521768904 20.397391779972352 9 26.145403098337994 17.624973620628023 21.7432781053366 34.48634517569739 10-11 24.357366197365078 27.195382856207654 27.369002192179646 21.078248754247618 12-13 26.44106740629401 25.042206995163426 27.454977410643917 21.06174818789865 14-15 23.27410938641909 26.09823162615293 27.34054823761539 23.287110749812584 16-17 21.14526902893321 29.384901754674036 27.705276135383254 21.764553081009495 18-19 21.079470084586628 28.493720605716057 29.827715041259665 20.599094268437646 20-21 22.744159930056977 26.758104389096815 29.27313524757848 21.224600433267724 22-23 22.07573894542808 26.797485022976968 29.37714534280263 21.749630688792323 24-25 22.32560731150645 27.080358361365924 29.406670917876372 21.187363409251255 26-27 22.069117184707284 26.731967276658242 30.11173009918644 21.087185439448035 28-29 21.26570380164435 27.76399291094755 29.715904930896812 21.254398356511288 30-31 21.817894043214796 27.094683641343902 29.467185137969498 21.6202371774718 32-33 21.70322452829376 27.164858154348398 29.4738338164168 21.658083500941043 34-35 22.424789005397695 26.952578374231063 29.254768410709236 21.367864209662006 36-37 22.265261110566655 26.707306960399674 29.836880608861314 21.190551320172354 38-39 21.963021889124892 27.064983591905516 29.66419266521834 21.307801853751247 40-41 21.941480627241706 27.121861611002895 29.507101855171904 21.4295559065835 42-43 21.59658257796351 27.30882321667622 28.824982563211893 22.269611642148384 44-45 22.104643757170322 26.952332807352747 28.73251060709573 22.2105128283812 46-47 22.538063441491776 27.281128145439197 28.31897974202836 21.861828671040666 48-49 22.25049563718147 27.284015919610543 28.378038259287226 22.08745018392076 50-51 21.751649595787455 27.515255042999986 28.82607568611691 21.907019675095647 52-53 22.148228382912198 27.99944226470677 27.729457467838703 22.122871884542334 54-55 21.57178526975869 27.25694369672982 28.353652627816135 22.817618405695363 56-57 22.309169469699253 27.590804258599675 27.715541003234435 22.384485268466637 58-59 22.75806191117093 27.176716016150742 27.94277254374159 22.122449528936745 60-61 22.28422363685942 27.324911728738517 28.5652989298161 21.825565704585962 62-63 22.106749603092783 27.32885882916004 28.44943194757218 22.114959620175 64-65 21.812965124870896 27.66290255416088 28.24750598721883 22.276626333749395 66-67 21.88514067705772 27.426891564760226 27.720921568579946 22.96704618960211 68-69 21.7001701779897 27.33679790811896 28.227957751066374 22.735074162824965 70-71 22.2503871443057 27.419480310081525 27.693179826881288 22.636952718731486 72-73 21.967691191227836 26.86677125100672 28.35868624267299 22.806851315092448 74-75 21.59525752727304 28.06043783297228 27.58507078285362 22.75923385690106 76-77 21.787837133701434 27.142048108958317 27.854937065861137 23.21517769147912 78-79 21.704902587570242 27.08661798849221 28.253359803492717 22.955119620444833 80-81 21.95045137074743 27.372849168657908 27.76450282730873 22.91219663328593 82-83 21.62629366593594 27.234709654634802 28.245577955889384 22.893418723539874 84-85 21.457650295287777 27.184537364536393 27.95554263503292 23.402269705142906 86-87 21.44530271230005 27.35411317460617 27.958648672678432 23.241935440415347 88-89 21.54736909836203 26.891035744661984 27.799183853558652 23.762411303417334 90-91 21.372666433555473 26.648473545032164 28.21411064927232 23.76474937214004 92-93 21.664786014841358 27.109649897281958 28.109993367472192 23.1155707204045 94-95 21.263181412576362 26.83887014348645 28.05900965952756 23.838938784409628 96-97 20.706245773916937 26.82268001498779 28.3206514951182 24.150422715977072 98-99 20.876123545904417 26.885829001373878 27.66014432208244 24.577903130639264 100-101 21.036407199232734 25.8384439114112 28.773539323075298 24.351609566280764 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 299.0 1 212.5 2 128.5 3 130.5 4 128.5 5 149.5 6 244.5 7 488.5 8 901.5 9 1240.5 10 1276.5 11 1291.5 12 1468.5 13 1600.0 14 1753.0 15 1962.0 16 2110.0 17 2300.0 18 2545.0 19 2757.5 20 3045.0 21 3523.5 22 4109.0 23 4775.5 24 5635.5 25 6956.5 26 9045.0 27 11564.5 28 13743.0 29 16247.5 30 19659.5 31 23474.5 32 28010.5 33 33220.5 34 39082.5 35 43732.5 36 49085.5 37 55779.0 38 58313.5 39 58112.5 40 59114.0 41 60903.0 42 62545.0 43 65334.0 44 68186.5 45 69075.5 46 70208.5 47 81954.5 48 110013.0 49 109214.5 50 81631.5 51 67720.0 52 59845.5 53 55646.5 54 51630.5 55 45926.5 56 40784.5 57 35784.0 58 31228.0 59 26735.5 60 22807.0 61 18661.0 62 14459.0 63 10776.0 64 7643.5 65 5605.0 66 3981.5 67 2999.5 68 2230.5 69 1502.0 70 1053.0 71 724.5 72 497.0 73 384.0 74 296.5 75 209.5 76 127.0 77 56.0 78 26.0 79 15.0 80 5.5 81 5.0 82 3.5 83 3.0 84 2.5 85 0.5 86 0.5 87 1.0 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3950983896739294 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.806140687112653E-4 16-17 0.0023956776591483662 18-19 4.306836241165602E-4 20-21 0.0 22-23 0.0 24-25 8.075317952185504E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012759002364453096 36-37 0.02185719392391543 38-39 0.010605584243870295 40-41 0.005921899831602703 42-43 0.004764437591789447 44-45 0.0015612281374225306 46-47 0.0011305445133059707 48-49 0.0029340321892940666 50-51 6.191077096675553E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.460254361748403E-4 60-61 0.0014804749579006757 62-63 0.0013458863253642506 64-65 7.267786156966953E-4 66-67 0.002611019471206646 68-69 0.002422595385655651 70-71 2.6917726507285013E-5 72-73 0.0 74-75 0.0 76-77 0.09060506742352135 78-79 0.004010741249585467 80-81 1.8842408555099508E-4 82-83 0.0 84-85 1.0767090602914005E-4 86-87 0.09189711829587104 88-89 0.4354749794348569 90-91 0.3864847171915982 92-93 0.0 94-95 0.0010497913337841156 96-97 0.0 98-99 0.0 100-101 4.576013506238452E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1857512.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.11585191775333 #Duplication Level Percentage of deduplicated Percentage of total 1 72.60351965948281 36.38587239962334 2 15.099477606959294 15.13446367571609 3 5.3041827515368904 7.974709119621726 4 2.3661714341919415 4.743307888319254 5 1.2726670299707472 3.1890396207310454 6 0.7528243360656343 2.263705976780778 7 0.5071024226239623 1.778970894354952 8 0.3442418763208039 1.3801579918066371 9 0.27246350115813645 1.22892664293304 >10 1.3211588634063132 12.013413051001983 >50 0.10336166562702448 3.542423795522128 >100 0.04658915764066737 4.141641842151466 >500 0.0024743618166598812 0.8426392364862143 >1k 0.003335009405063318 3.71410401964696 >5k 3.2274284565128884E-4 1.0831006220341393 >10k+ 1.075809485504296E-4 0.5835232232702219 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10823 0.5826611079766915 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7462 0.40172015039472153 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6693 0.3603206870265172 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5934 0.31945957818845855 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4862 0.26174797255683946 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4011 0.21593400204144036 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3837 0.20656663321690522 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3714 0.19994487249611306 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3586 0.1930539345102481 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3264 0.17571891863955655 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3120 0.1679666134054585 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3021 0.16263690355701604 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3018 0.16247539719797233 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2903 0.15628432010129678 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2902 0.15623048464828224 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2894 0.15579980102416566 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2387 0.12850522634577866 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2336 0.12575961824203558 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1906 0.10261037344577047 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.3835453014570025E-5 0.0 14-15 0.0 0.0 0.0 1.3458863253642506E-4 0.0 16-17 0.0 0.0 0.0 2.691772650728501E-4 0.0 18-19 0.0 0.0 0.0 2.691772650728501E-4 0.0 20-21 0.0 0.0 0.0 3.2301271808742016E-4 0.0 22-23 0.0 0.0 0.0 4.8451907713113025E-4 0.0 24-25 0.0 0.0 0.0 7.267786156966954E-4 0.0 26-27 0.0 0.0 0.0 0.0013728040518715358 0.0 28-29 0.0 0.0 0.0 0.0037146462580053318 0.0 30-31 0.0 0.0 0.0 0.010713255149899435 0.0 32-33 0.0 0.0 0.0 0.022530137086597554 0.0 34-35 0.0 0.0 0.0 0.041722476086291774 0.0 36-37 0.0 0.0 0.0 0.06904396849118606 0.0 38-39 0.0 0.0 0.0 0.11348513495471363 0.0 40-41 0.0 0.0 0.0 0.17160050648394198 0.0 42-43 0.0 0.0 0.0 0.23491099922907632 0.0 44-45 0.0 0.0 0.0 0.3181944450426161 0.0 46-47 0.0 0.0 0.0 0.41318710188682495 0.0 48-49 0.0 0.0 0.0 0.5161204880506829 0.0 50-51 0.0 0.0 0.0 0.6314360284078919 0.0 52-53 0.0 0.0 0.0 0.7510853227327737 0.0 54-55 0.0 0.0 0.0 0.8862392275258517 0.0 56-57 0.0 0.0 0.0 1.0507065364853632 0.0 58-59 0.0 0.0 0.0 1.2251064865260628 0.0 60-61 0.0 0.0 0.0 1.4259396439969163 0.0 62-63 0.0 0.0 0.0 1.646530412724117 0.0 64-65 0.0 0.0 0.0 1.8743620498817775 0.0 66-67 0.0 0.0 0.0 2.1425702768003654 0.0 68-69 0.0 0.0 0.0 2.4469020926917295 0.0 70-71 0.0 0.0 0.0 2.783723604477387 0.0 72-73 0.0 0.0 0.0 3.138364651210867 0.0 74-75 0.0 0.0 0.0 3.530905856866604 0.0 76-77 0.0 0.0 0.0 3.942154882444905 0.0 78-79 0.0 0.0 0.0 4.422986231044536 0.0 80-81 0.0 0.0 0.0 4.9396989090783805 0.0 82-83 0.0 0.0 0.0 5.466640323184992 0.0 84-85 0.0 0.0 0.0 6.051939368359397 0.0 86-87 0.0 0.0 0.0 6.658449582021543 0.0 88-89 0.0 0.0 0.0 7.30609008178682 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 6880 0.0 24.626959 12-13 GTACTTT 7485 0.0 22.477884 14-15 AGTACTT 7230 0.0 20.415176 12-13 CTTTGCG 585 0.0 20.279474 2 TCGCGCT 85 8.0178106E-5 19.540396 60-61 GTACATG 9875 0.0 19.49331 1 ACTTTTT 8705 0.0 19.326065 16-17 TACATGG 10170 0.0 18.524313 2 TATCCCG 130 0.0076072486 18.251528 4 TTGCGTT 705 0.0 18.17386 4 ACATGGG 10315 0.0 17.435827 3 TTTGCGT 685 0.0 17.318968 3 CATGGGG 3730 0.0 16.920584 4 TACTTTT 8675 0.0 16.795769 14-15 GTAAGCG 235 1.4551915E-11 16.154543 94-95 CTCCGAC 385 0.0 16.023418 94-95 AGAGTAC 18960 0.0 14.843879 10-11 CGCGGAA 115 7.692179E-4 14.529533 82-83 GACATAT 630 0.0 14.369923 1 TAATACC 595 3.6379788E-12 14.355823 4 >>END_MODULE