FastQCFastQC Report
Thu 26 May 2016
SRR938918_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938918_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862324
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT106280.5706848002817985No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77620.4167910632091945No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT71620.384573253633632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55790.29957193270343935No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT50540.2713813493248221No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38950.20914728049469372No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38380.20608658858501527No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33930.18219171314980637No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32870.17649990012479033No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32440.17419095710520832No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC32400.17397617170803792No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30140.16184079676790933No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28680.15400112977118913No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA28340.15217545389524056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28270.15179957945019232No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT26310.1412750949888419No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG25870.13891245561996732No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA20230.10862771461893848No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT63250.024.24475312-13
TTTGCGT6750.022.437463
TTGCGTT7000.022.312254
GTACTTT69100.022.19189614-15
CTTTGCG6850.022.1099032
AGTACTT64950.021.13256312-13
CGCACCG600.00472281919.82244566-67
ACTTTTT82150.019.33043516-17
GTACATG108900.019.1982861
TACATGG111750.018.5927852
CATTCCG4101.8189894E-1218.4703569
CGTATAG1300.00764987118.233851
ACATGGG112100.017.6903293
TACTTTT80550.017.30402814-15
CATGGGG35850.015.5783094
GTATCAA312700.015.5550441
GTACACT9650.015.2037326
CATGGGA74550.015.0462474
AGAGTAC190900.014.9503210-11
TTAGGCA7700.014.7519014
Produced by FastQC (version 0.11.5)