##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938918_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1862324 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.896448201279693 33.0 31.0 34.0 30.0 34.0 2 31.965290142853767 33.0 31.0 34.0 30.0 34.0 3 32.00323627897186 34.0 31.0 34.0 30.0 34.0 4 35.53550832186021 37.0 35.0 37.0 33.0 37.0 5 35.578265650874926 37.0 35.0 37.0 33.0 37.0 6 35.66648982668966 37.0 35.0 37.0 33.0 37.0 7 35.59622976453077 37.0 35.0 37.0 33.0 37.0 8 35.584015992920676 37.0 35.0 37.0 33.0 37.0 9 37.19320429742623 39.0 37.0 39.0 34.0 39.0 10-11 37.09918172133313 39.0 37.0 39.0 33.0 39.0 12-13 36.99089954272189 39.0 37.0 39.0 33.0 39.0 14-15 38.18868467570627 40.0 38.0 41.0 33.0 41.0 16-17 38.12949250506357 40.0 38.0 41.0 33.0 41.0 18-19 38.115149941685765 40.0 38.0 41.0 33.0 41.0 20-21 38.01230612933088 40.0 38.0 41.0 33.0 41.0 22-23 38.08967156090992 40.0 38.0 41.0 33.0 41.0 24-25 38.0214511545789 40.0 38.0 41.0 33.0 41.0 26-27 37.943141472697555 40.0 38.0 41.0 33.0 41.0 28-29 37.82899672667055 40.0 38.0 41.0 33.0 41.0 30-31 37.66168829913592 40.0 37.5 41.0 32.0 41.0 32-33 37.478787525693704 40.0 37.0 41.0 32.0 41.0 34-35 37.17845793750175 40.0 36.5 41.0 31.0 41.0 36-37 36.913981133250715 40.0 36.0 41.0 30.5 41.0 38-39 36.71876537057999 39.5 36.0 41.0 30.0 41.0 40-41 36.59474989314427 39.0 36.0 41.0 30.0 41.0 42-43 36.39138973669458 39.0 35.5 41.0 30.0 41.0 44-45 36.30013869767022 39.5 36.0 41.0 30.0 41.0 46-47 36.04429035978702 40.0 36.0 41.0 28.5 41.0 48-49 35.843811012476884 39.0 35.5 41.0 27.5 41.0 50-51 34.67625934047996 38.0 34.0 40.0 25.5 40.5 52-53 34.82656562445632 38.5 34.5 39.5 26.0 40.5 54-55 35.342782458906186 39.0 35.0 40.5 26.0 41.0 56-57 35.2622016899315 39.0 35.0 41.0 26.0 41.0 58-59 35.084580878515226 39.0 35.0 41.0 26.0 41.0 60-61 34.80455656480827 38.5 34.5 40.0 26.0 41.0 62-63 34.47503978899483 38.0 34.0 40.0 25.0 41.0 64-65 34.08295146279595 37.5 34.0 40.0 23.0 41.0 66-67 33.691499706817936 37.0 34.0 39.5 22.5 41.0 68-69 33.299680399329006 36.0 33.0 39.0 22.0 41.0 70-71 32.8602893481478 36.0 33.0 39.0 21.5 40.5 72-73 32.41727943150602 35.0 32.0 38.0 20.5 40.0 74-75 31.952830978927405 35.0 32.0 37.0 18.5 39.5 76-77 31.535118486364347 35.0 31.5 37.0 18.0 39.0 78-79 31.104598609049766 35.0 31.0 36.0 15.0 39.0 80-81 30.670444562815064 35.0 31.0 36.0 9.5 37.5 82-83 30.290449728403864 34.0 31.0 35.5 7.0 37.0 84-85 29.946139608360305 34.0 30.0 35.0 3.5 37.0 86-87 29.591085117305045 34.0 30.0 35.0 2.0 36.0 88-89 29.181383314611207 34.0 29.5 35.0 2.0 36.0 90-91 28.759172410386164 34.0 29.0 35.0 2.0 35.5 92-93 28.38055703518829 34.0 29.0 35.0 2.0 35.0 94-95 27.93153232198049 34.0 29.0 35.0 2.0 35.0 96-97 27.37269991687805 34.0 27.0 35.0 2.0 35.0 98-99 26.716984531155695 33.5 25.5 35.0 2.0 35.0 100-101 24.129389139591176 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3768880521044373 1101 2 0.36733067927535856 1101 3 0.3211905845418954 1101 4 0.24191127564499482 1101 5 0.10495588113293053 1101 6 0.25281912016153285 1101 7 0.312618184097083 1101 8 0.37342362476662316 1101 9 0.48332761085198683 1101 10-11 0.478220911735427 1101 12-13 0.49526277371173677 1101 14-15 0.2078661264381907 1101 16-17 0.49951812131212847 1101 18-19 0.45769657658910745 1101 20-21 0.6431590478073659 1101 22-23 0.664853977442597 1101 24-25 0.7114339446949245 1101 26-27 0.4848805319466365 1101 28-29 0.5301421519849896 1101 30-31 0.5547985101672239 1101 32-33 0.5632269868020288 1101 34-35 0.5057099444119686 1101 36-37 0.532215145491719 1101 38-39 0.6298720430031963 1101 40-41 0.4838701211977465 1101 42-43 0.4599116435585202 1101 44-45 0.502123827315998 1101 46-47 0.5322636803745624 1101 48-49 0.6025040267870594 1101 50-51 0.44834735820207783 1101 52-53 0.4512194320994496 1101 54-55 0.4722894052218649 1101 56-57 0.5165311757932685 1101 58-59 0.33671184373353924 1101 60-61 0.2786944698804774 1101 62-63 0.3732544731570897 1101 64-65 0.37722019310935195 1101 66-67 0.2353835341270667 1101 68-69 0.16134262563715396 1101 70-71 0.1361412084487199 1101 72-73 0.1581988577905591 1101 74-75 0.17489185235503513 1101 76-77 0.14352524324787552 1101 78-79 0.1635407942370044 1101 80-81 0.09869234006952965 1101 82-83 0.016922429258976024 1101 84-85 -0.2716274114460262 1101 86-87 -0.15431325763459114 1101 88-89 -0.26079969333583364 1101 90-91 -0.18477277033760942 1101 92-93 -0.12193661867011585 1101 94-95 -0.2092655092142941 1101 96-97 -0.347221411597058 1101 98-99 -0.38873742834753955 1101 100-101 -0.10530509992172554 1102 1 0.3678288977768922 1102 2 0.3042141405081331 1102 3 0.17481685101354572 1102 4 0.19977124746036878 1102 5 0.3777446900985808 1102 6 0.33551061868885057 1102 7 0.36498949989182705 1102 8 0.3322029364472101 1102 9 0.5457652473504169 1102 10-11 0.4028457289026761 1102 12-13 0.3439198295533643 1102 14-15 0.4896842020023797 1102 16-17 0.3766439903938519 1102 18-19 0.6388100364193292 1102 20-21 0.6787297865518838 1102 22-23 0.5161333058202473 1102 24-25 0.5384973667898052 1102 26-27 0.6138096830015556 1102 28-29 0.5973843661480984 1102 30-31 0.6261497026709222 1102 32-33 0.5408554372485028 1102 34-35 0.45871925469000274 1102 36-37 0.5244647063591685 1102 38-39 0.48379117582493336 1102 40-41 0.5375374624254405 1102 42-43 0.3224482935949169 1102 44-45 0.5603718386967103 1102 46-47 0.7165747932666875 1102 48-49 0.5032328532512267 1102 50-51 0.3594275241040137 1102 52-53 0.5665828935993815 1102 54-55 0.6332174609499575 1102 56-57 0.6166933042003038 1102 58-59 0.6596781423971123 1102 60-61 0.6168667768124223 1102 62-63 0.4995855624061889 1102 64-65 0.5945744445135759 1102 66-67 0.49197503065073533 1102 68-69 0.34465057944641586 1102 70-71 0.6244076867726065 1102 72-73 0.44281452497148877 1102 74-75 0.3867783190577647 1102 76-77 0.31376457883864717 1102 78-79 0.16123225937021246 1102 80-81 0.03545792009183657 1102 82-83 -0.02311360298638121 1102 84-85 0.015095925026354706 1102 86-87 0.13405106543338974 1102 88-89 0.06978513772336115 1102 90-91 0.256464782007658 1102 92-93 0.32471645659353143 1102 94-95 0.3017852698703045 1102 96-97 0.1787844190146508 1102 98-99 0.07901738541350412 1102 100-101 0.23641650005690096 1103 1 0.0034610816849394155 1103 2 0.07375437039319266 1103 3 0.1558513337721621 1103 4 0.2583919371155403 1103 5 0.2530320464204223 1103 6 0.18436119340149304 1103 7 0.1598170860987267 1103 8 0.1511684536885909 1103 9 0.1969146726377673 1103 10-11 0.3212365335003753 1103 12-13 0.41231063415106917 1103 14-15 0.29140833993341886 1103 16-17 0.3002072087846628 1103 18-19 0.3333502663043859 1103 20-21 0.439074614138093 1103 22-23 0.3488919265099 1103 24-25 0.47010656219210034 1103 26-27 0.44628094736937385 1103 28-29 0.33675218224004766 1103 30-31 0.4014370589927623 1103 32-33 0.47620026483470923 1103 34-35 0.32940890986241556 1103 36-37 0.3750394190028459 1103 38-39 0.3849406011122909 1103 40-41 0.2754684969081964 1103 42-43 0.13365519014664784 1103 44-45 0.2908316088116507 1103 46-47 0.31226444843910883 1103 48-49 0.07823285325122242 1103 50-51 0.06919763904654275 1103 52-53 0.11629553727754427 1103 54-55 0.2826427483062801 1103 56-57 0.32502663753363237 1103 58-59 0.15421837228217328 1103 60-61 0.20853344347909086 1103 62-63 0.11452809114182827 1103 64-65 0.04256295026070234 1103 66-67 0.11668767432890093 1103 68-69 -0.03753332859955805 1103 70-71 -0.013235991388320656 1103 72-73 0.03936624910942754 1103 74-75 0.1017208477933984 1103 76-77 0.0011209006777299635 1103 78-79 0.10462306396791732 1103 80-81 0.10298665572402399 1103 82-83 0.19700133954235355 1103 84-85 0.05590052272750157 1103 86-87 0.19037290451385047 1103 88-89 0.11288858599922591 1103 90-91 0.210200414191565 1103 92-93 -0.014651359498419225 1103 94-95 -0.045915879554979 1103 96-97 -0.07782477638764718 1103 98-99 -0.22241939619568996 1103 100-101 0.025209603505178535 1104 1 0.16938101287580665 1104 2 0.23159313808417537 1104 3 0.25931915768333624 1104 4 0.27270501867000263 1104 5 0.18477025894681276 1104 6 0.23694544549313434 1104 7 0.1938815164685721 1104 8 0.2689024438363745 1104 9 0.3958942644745065 1104 10-11 0.3442230844425893 1104 12-13 0.45061308156681434 1104 14-15 0.35672423640694717 1104 16-17 0.3306005155326446 1104 18-19 0.38493756789169 1104 20-21 0.4151008867160897 1104 22-23 0.4204847015509543 1104 24-25 0.4468404741476846 1104 26-27 0.3855392943863407 1104 28-29 0.4694267463185611 1104 30-31 0.4926638945545676 1104 32-33 0.45531317436634566 1104 34-35 0.39047115085076456 1104 36-37 0.3717279794845112 1104 38-39 0.3860998004241978 1104 40-41 0.4985762457548688 1104 42-43 0.41312778378179615 1104 44-45 0.5363199190782879 1104 46-47 0.5978340785900187 1104 48-49 0.5877507983603039 1104 50-51 0.5529844871191116 1104 52-53 0.536641146785712 1104 54-55 0.5803165304628166 1104 56-57 0.6915857899302296 1104 58-59 0.7108119292451818 1104 60-61 0.6296179692434194 1104 62-63 0.6499608849490031 1104 64-65 0.5378284127675457 1104 66-67 0.6319605650944027 1104 68-69 0.5810705497333899 1104 70-71 0.6834349758491598 1104 72-73 0.4911724888466935 1104 74-75 0.5657602566603934 1104 76-77 0.5875760574530879 1104 78-79 0.7125107017816639 1104 80-81 0.5782036390690841 1104 82-83 0.5038392471191813 1104 84-85 0.6958997408055403 1104 86-87 0.8043458500138883 1104 88-89 0.795676528762538 1104 90-91 0.9027643363121385 1104 92-93 0.850183654967136 1104 94-95 0.9925879127665418 1104 96-97 0.9869848256140763 1104 98-99 0.7520137103802753 1104 100-101 1.097467012215791 1105 1 0.05969484708985462 1105 2 0.0965214155213765 1105 3 0.1428620621669907 1105 4 0.131128028814274 1105 5 0.2042341244485968 1105 6 0.3165163817706613 1105 7 0.23696100997243263 1105 8 0.18028765311164818 1105 9 0.2910753241276822 1105 10-11 0.18862374494739953 1105 12-13 0.3217937118792733 1105 14-15 0.4198266196885996 1105 16-17 0.32800290976815205 1105 18-19 0.3788805272705176 1105 20-21 0.3884159762166348 1105 22-23 0.3720658337262037 1105 24-25 0.4800194777498703 1105 26-27 0.3840575338124168 1105 28-29 0.3704097288137973 1105 30-31 0.5053694209628006 1105 32-33 0.3160800889426483 1105 34-35 0.4382151605405227 1105 36-37 0.43807559941347307 1105 38-39 0.4938669957717039 1105 40-41 0.38107617335870003 1105 42-43 0.36165049288394613 1105 44-45 0.39462866919318174 1105 46-47 0.5482082408405162 1105 48-49 0.6458265272637504 1105 50-51 0.7194937708251885 1105 52-53 0.6847035736813325 1105 54-55 0.46200813918107286 1105 56-57 0.5793929857309976 1105 58-59 0.5676042945348101 1105 60-61 0.7912046287082433 1105 62-63 0.5346352155363192 1105 64-65 0.4486925270603095 1105 66-67 0.4521939703202662 1105 68-69 0.3888906887088339 1105 70-71 0.4024609075248051 1105 72-73 0.5324061949887877 1105 74-75 0.6277132721808663 1105 76-77 0.441950548319209 1105 78-79 0.39850242217886844 1105 80-81 0.3558380409697648 1105 82-83 0.497736722822399 1105 84-85 0.42922265538688364 1105 86-87 0.44511436360107837 1105 88-89 0.4937930324434099 1105 90-91 0.47876991900051635 1105 92-93 0.6134766919672394 1105 94-95 0.7226076737710763 1105 96-97 0.6661779168798212 1105 98-99 0.7950338705079929 1105 100-101 1.1092875742366353 1106 1 0.242277467054965 1106 2 0.20468551969090854 1106 3 0.2543083483281521 1106 4 0.24283961338839077 1106 5 0.2010144924892856 1106 6 0.1605689829584378 1106 7 0.19930003607767333 1106 8 0.22535294856656662 1106 9 0.2375177063040681 1106 10-11 0.2788991561291283 1106 12-13 0.27526526948600605 1106 14-15 0.31985980509983847 1106 16-17 0.5372136639366616 1106 18-19 0.38606946719228574 1106 20-21 0.4034207429604706 1106 22-23 0.3975868631703179 1106 24-25 0.32682674538574474 1106 26-27 0.3100389684698115 1106 28-29 0.19601392206936197 1106 30-31 0.17088990193865783 1106 32-33 0.3172708505185611 1106 34-35 0.37429549819977126 1106 36-37 0.1875587142941555 1106 38-39 0.3616280620433159 1106 40-41 0.44735733051581406 1106 42-43 0.2876590428262986 1106 44-45 0.40696566165205184 1106 46-47 0.19600888310301912 1106 48-49 0.16494110844659815 1106 50-51 0.06246437970507657 1106 52-53 0.03232306735267798 1106 54-55 -0.1206132125035495 1106 56-57 -0.001026789692815555 1106 58-59 0.030016655160707728 1106 60-61 0.15819984224419414 1106 62-63 0.056241321651988585 1106 64-65 -0.2197799765001136 1106 66-67 -0.0010203002076920598 1106 68-69 0.051360237935710984 1106 70-71 -0.05526081693336948 1106 72-73 -0.24866684311250253 1106 74-75 -0.17396676622422902 1106 76-77 -0.2306487167333202 1106 78-79 -0.052969075034724256 1106 80-81 -0.04486917496218723 1106 82-83 -0.06598774027955301 1106 84-85 0.08465178250272132 1106 86-87 0.10655384012817493 1106 88-89 0.3577126639713377 1106 90-91 0.28799201228818916 1106 92-93 0.15118927734187082 1106 94-95 0.25578784682821976 1106 96-97 0.2535222581971688 1106 98-99 0.3521459016215509 1106 100-101 0.8949064282337673 1107 1 0.10934927682787432 1107 2 -0.020381473077478063 1107 3 0.10996130410345728 1107 4 0.06996589697105549 1107 5 0.03812051484443657 1107 6 0.04448809197752013 1107 7 -0.07775621585788883 1107 8 0.03241896767210761 1107 9 0.17760628885497454 1107 10-11 0.1219317555096282 1107 12-13 0.040556479582782856 1107 14-15 0.047926125344517345 1107 16-17 0.025836957201583743 1107 18-19 -0.08189464288658854 1107 20-21 0.001579121378142645 1107 22-23 -0.06639188727940848 1107 24-25 -0.03200741246613603 1107 26-27 -0.07031961310988066 1107 28-29 0.06788914540403823 1107 30-31 0.08890629905626923 1107 32-33 -0.0073475182357753965 1107 34-35 -0.1423746603777758 1107 36-37 -0.11140311922171264 1107 38-39 -0.11109334394050308 1107 40-41 -0.16184164548895552 1107 42-43 -0.08844388689711735 1107 44-45 0.086263323334137 1107 46-47 0.14748623627936297 1107 48-49 -0.16313791705982084 1107 50-51 -0.2427909868936453 1107 52-53 -0.3114080160091106 1107 54-55 -0.2650908216176262 1107 56-57 -0.2200913098217967 1107 58-59 -0.2387946148951272 1107 60-61 -0.2948129845727081 1107 62-63 -0.28239986892877056 1107 64-65 -0.0237341227217982 1107 66-67 -0.2462506349974518 1107 68-69 -0.23146041568892173 1107 70-71 -0.06315976367938703 1107 72-73 -0.21189641069991438 1107 74-75 -0.1136399844487066 1107 76-77 -0.23519970484229447 1107 78-79 0.02573070634925756 1107 80-81 -0.1404142703951976 1107 82-83 -0.018941163889472534 1107 84-85 -0.09233342991642601 1107 86-87 -0.01161486742296347 1107 88-89 0.01664983828447575 1107 90-91 0.050036508802488555 1107 92-93 -0.0641076440143884 1107 94-95 0.13160643523698923 1107 96-97 0.24670188433262652 1107 98-99 0.37684679349427697 1107 100-101 0.6904448656209574 1108 1 0.13586745914136955 1108 2 0.1194718342419101 1108 3 -0.012020375534468997 1108 4 0.08445793637397259 1108 5 0.05429270641300832 1108 6 0.07683431168106125 1108 7 0.13829317063709823 1108 8 0.08316697055919064 1108 9 0.09671074234485388 1108 10-11 0.22003149086039997 1108 12-13 0.09099991854112943 1108 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-1.2707696816117995 2316 80-81 -1.2401130015713413 2316 82-83 -1.454238311080882 2316 84-85 -1.391843843875474 2316 86-87 -1.417773912578923 2316 88-89 -1.5644997317152693 2316 90-91 -1.84523433545208 2316 92-93 -2.111821771653819 2316 94-95 -2.2092358686369558 2316 96-97 -2.5675072438610336 2316 98-99 -3.097133556266048 2316 100-101 -3.7156518589034597 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5826.0 3 1285.0 4 1204.0 5 1362.0 6 1620.0 7 1988.0 8 2487.0 9 2936.0 10 3742.0 11 4978.0 12 6927.0 13 10639.0 14 10496.0 15 9960.0 16 13215.0 17 18077.0 18 15687.0 19 10001.0 20 9727.0 21 10272.0 22 11121.0 23 12436.0 24 14631.0 25 16461.0 26 19289.0 27 23205.0 28 27219.0 29 33076.0 30 40132.0 31 49115.0 32 61826.0 33 79954.0 34 108742.0 35 153256.0 36 237006.0 37 386935.0 38 387654.0 39 57833.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.55160923452046 20.340683413102198 16.104892107405796 21.002815244971547 2 14.896272116272396 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22.888937981271077 26.723680122637788 29.212018890175322 21.175363005915813 22-23 22.107551806636938 26.775894448504616 29.39597427280407 21.72057947205437 24-25 22.35261764418878 27.05300880357272 29.31528693668519 21.27908661555331 26-27 22.063010520571368 26.647490291960306 30.08644154140417 21.20305764606416 28-29 21.272674586453068 27.691314345128426 29.723741134055924 21.31226993436258 30-31 21.79321771123289 27.041126804780998 29.466056839110678 21.699598644875437 32-33 21.717227644002698 27.080893084161385 29.457666660395258 21.74421261144066 34-35 22.442790883193954 26.902150917259355 29.21091709764965 21.444141101897042 36-37 22.278032161756702 26.66598250804449 29.787798301036094 21.26818702916271 38-39 21.978765860125772 27.076174506611366 29.565839604479045 21.379220028783816 40-41 21.97993456559757 27.153468533480673 29.460785650102206 21.40581125081955 42-43 21.552874854205363 27.341731824268866 28.8123947038749 22.29299861765087 44-45 22.03593739324681 26.90867611599192 28.77934978481258 22.27603670594869 46-47 22.493619425029998 27.244407073662565 28.384134915003074 21.877838586304364 48-49 22.283565707569288 27.203098971330785 28.418985547640503 22.094349773459427 50-51 21.796695708439398 27.457086824476324 28.826673493670707 21.91954397341357 52-53 22.0946178783273 27.924286468916392 27.840972309992406 22.1401233427639 54-55 21.588680060488226 27.19907107366602 28.435191186001298 22.77705767984446 56-57 22.36965755031487 27.51805169397199 27.735263238741297 22.377027516971847 58-59 22.776387976677256 27.05366395932153 28.026137798981583 22.14381026501964 60-61 22.340651139599526 27.189662364264972 28.68085223492311 21.78883426121239 62-63 22.11248149762203 27.187168732506784 28.56359444839558 22.13675532147561 64-65 21.814527172477423 27.571139259925896 28.343560071802347 22.270773495794334 66-67 21.990719480662612 27.307136675069447 27.78942329822029 22.912720546047648 68-69 21.69814761138225 27.26568660317261 28.34073815145809 22.695427633987048 70-71 22.32776639947617 27.31102129726325 27.767101803414285 22.5941104998463 72-73 22.091076924739113 26.782217864517705 28.51664728658615 22.610057924157033 74-75 21.69734088914686 27.98150282397675 27.768722097513425 22.552434189362963 76-77 22.02842536013747 27.013841085552887 27.954385692963008 23.003347861346633 78-79 21.904252004559634 27.08400493978356 28.31041906622539 22.701323989431412 80-81 22.163594374246415 27.39450092144474 27.82954811140275 22.612356592906092 82-83 21.91858904674034 27.195244907360156 28.30549736707239 22.580668678827116 84-85 21.85235103038761 27.21463867910338 27.949748333905372 22.98326195660363 86-87 21.822796704676474 27.401786992058714 27.998720105415792 22.776696197849024 88-89 22.01813382302307 26.93865150788882 27.848637262778404 23.194577406309705 90-91 21.850173219440013 26.743288168708467 28.257766515805816 23.148772096045704 92-93 22.264751903639166 27.191754421836876 28.105873966946053 22.437619707577902 94-95 21.921591146659118 26.9841389887221 27.99862031188298 23.095649552735797 96-97 21.42184406112748 26.946237819596742 28.286544721057727 23.345373398218054 98-99 21.71576265917066 27.00275536115057 27.671891345691808 23.609590633986965 100-101 21.994169108806936 26.31632530712637 28.353923645354783 23.33558193871191 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1770.0 1 1000.0 2 233.0 3 227.5 4 199.0 5 232.5 6 413.5 7 737.0 8 1206.5 9 1575.5 10 1674.5 11 1776.5 12 1972.0 13 2215.5 14 2423.5 15 2638.5 16 2935.5 17 3097.0 18 3196.5 19 3281.0 20 3440.0 21 3787.5 22 4279.5 23 4927.5 24 5706.5 25 6903.0 26 8986.5 27 11464.5 28 13725.5 29 16362.5 30 19689.5 31 23687.0 32 28153.0 33 33152.0 34 39115.0 35 44134.5 36 49227.5 37 55671.0 38 58672.5 39 58580.5 40 59240.5 41 60569.5 42 62106.5 43 64802.0 44 67597.5 45 68621.5 46 69999.5 47 81063.5 48 109516.0 49 109149.5 50 80035.5 51 66034.5 52 59402.0 53 55453.5 54 51530.5 55 45843.0 56 40311.0 57 35438.0 58 31147.0 59 26826.0 60 22745.0 61 18763.0 62 14767.5 63 10936.5 64 7749.5 65 5715.0 66 4220.5 67 3202.0 68 2275.5 69 1599.0 70 1156.5 71 799.5 72 590.5 73 462.0 74 357.0 75 246.5 76 155.0 77 87.5 78 48.0 79 38.0 80 26.5 81 16.0 82 14.5 83 16.5 84 12.0 85 10.0 86 6.5 87 6.0 88 7.5 89 7.0 90 4.5 91 2.5 92 2.5 93 2.0 94 2.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28416108045646193 2 0.08537719537524083 3 0.08918963617501574 4 0.10454679207270057 5 0.10658725334581952 6 0.08097409473324728 7 0.09106900840025688 8 0.08720287125118938 9 0.09310946967337586 10-11 0.08569937347099646 12-13 0.09063943760591606 14-15 0.0999557542081829 16-17 0.08765929022017652 18-19 0.0988549790476845 20-21 0.1012713147658517 22-23 0.11348723422991917 24-25 0.12567630551934036 26-27 0.1286833010797262 28-29 0.1211926603534079 30-31 0.11415843859607672 32-33 0.10894989271469412 34-35 0.37957949314941974 36-37 0.5853707518133258 38-39 0.5685906426593869 40-41 0.5334463820473774 42-43 0.558656818040255 44-45 0.5798400278361875 46-47 0.5697182659945316 48-49 0.5906598422186472 50-51 0.5827664788726344 52-53 0.5851828145908016 54-55 0.5758396498138885 56-57 0.5483739671507213 58-59 0.5445078300016538 60-61 0.4925566120610592 62-63 0.5656910397975863 64-65 0.5976940639759785 66-67 0.6094535644710588 68-69 0.6115745702681167 70-71 0.6086481192316697 72-73 0.6012111748546439 74-75 0.5972376450069913 76-77 0.5822563635543547 78-79 0.5595696559782293 80-81 0.555273948034821 82-83 0.5682684645636312 84-85 0.5779606556109462 86-87 0.569664569645239 88-89 0.5805380803769913 90-91 0.5938547750015571 92-93 0.6013185675532292 94-95 0.6003788814406086 96-97 0.5983652683421359 98-99 0.6016944419982774 100-101 0.580242750455882 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1862324.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.67496841506116 #Duplication Level Percentage of deduplicated Percentage of total 1 74.00346036708619 38.241264770744095 2 14.439312767358276 14.923020623768563 3 4.986160083099825 7.729789944198666 4 2.2296883498488143 4.608763002154693 5 1.1971565161034454 3.0931512578765092 6 0.7342062582123178 2.2764051121957056 7 0.4627411493571942 1.6738493994167474 8 0.32108793422419335 1.327376708759394 9 0.2360749905008336 1.0979250909944795 >10 1.2459221100824598 11.695408393969183 >50 0.09568333344461002 3.4190447997416165 >100 0.04256098014686819 3.8508509353076543 >500 0.0025032254884322955 0.8636541946506044 >1k 0.0029204297365043445 3.2492132504063713 >5k 4.172042480720492E-4 1.3774594516154206 >10k+ 1.043010620180123E-4 0.5728230642003636 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10628 0.5706848002817985 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7762 0.4167910632091945 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7162 0.384573253633632 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5579 0.29957193270343935 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5054 0.2713813493248221 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3895 0.20914728049469372 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3838 0.20608658858501527 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3393 0.18219171314980637 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3287 0.17649990012479033 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3244 0.17419095710520832 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3240 0.17397617170803792 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3014 0.16184079676790933 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2868 0.15400112977118913 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2834 0.15217545389524056 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2827 0.15179957945019232 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2631 0.1412750949888419 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2587 0.13891245561996732 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2023 0.10862771461893848 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.3696349292604296E-5 0.0 10-11 0.0 0.0 0.0 5.3696349292604296E-5 0.0 12-13 0.0 0.0 0.0 1.6108904787781288E-4 0.0 14-15 0.0 0.0 0.0 1.8793722252411502E-4 0.0 16-17 0.0 0.0 0.0 2.1478539717041718E-4 0.0 18-19 0.0 0.0 0.0 2.1478539717041718E-4 0.0 20-21 0.0 0.0 0.0 3.2217809575562576E-4 0.0 22-23 0.0 0.0 0.0 4.2957079434083437E-4 0.0 24-25 0.0 0.0 0.0 8.054452393890645E-4 0.0 26-27 0.0 0.0 0.0 0.0015303459548392225 0.0 28-29 0.0 0.0 0.0 0.004349404292700948 0.0 30-31 0.0 0.0 0.0 0.011678955971141434 0.0 32-33 0.0 0.0 0.0 0.02523728416752402 0.0 34-35 0.0 0.0 0.0 0.04537341515225063 0.0 36-37 0.0 0.0 0.0 0.07530912988287752 0.0 38-39 0.0 0.0 0.0 0.12578369821792557 0.0 40-41 0.0 5.3696349292604296E-5 0.0 0.18804461522270022 0.0 42-43 0.0 5.3696349292604296E-5 0.0 0.2573666021594524 0.0 44-45 0.0 5.3696349292604296E-5 0.0 0.3409181216587447 0.0 46-47 0.0 5.3696349292604296E-5 0.0 0.4381085138783585 0.0 48-49 0.0 5.3696349292604296E-5 0.0 0.5447226153988243 0.0 50-51 0.0 5.3696349292604296E-5 0.0 0.6598207401075216 0.0 52-53 0.0 5.3696349292604296E-5 0.0 0.7794830545060902 0.0 54-55 0.0 5.3696349292604296E-5 0.0 0.9143414357544659 5.3696349292604296E-5 56-57 0.0 5.3696349292604296E-5 0.0 1.0787596572884204 5.3696349292604296E-5 58-59 0.0 5.3696349292604296E-5 0.0 1.2605754959931783 5.3696349292604296E-5 60-61 0.0 5.3696349292604296E-5 0.0 1.4666620845781937 5.3696349292604296E-5 62-63 0.0 5.3696349292604296E-5 0.0 1.6848571999286912 5.3696349292604296E-5 64-65 0.0 5.3696349292604296E-5 0.0 1.919241764590909 5.3696349292604296E-5 66-67 0.0 5.3696349292604296E-5 0.0 2.1855219607329337 5.3696349292604296E-5 68-69 0.0 5.3696349292604296E-5 0.0 2.4933362830527876 5.3696349292604296E-5 70-71 0.0 5.3696349292604296E-5 0.0 2.8362143214607123 5.3696349292604296E-5 72-73 0.0 5.3696349292604296E-5 0.0 3.188381828296258 5.3696349292604296E-5 74-75 0.0 5.3696349292604296E-5 0.0 3.5815733459913526 5.3696349292604296E-5 76-77 0.0 5.3696349292604296E-5 0.0 3.988376888232123 5.3696349292604296E-5 78-79 0.0 5.3696349292604296E-5 0.0 4.464448721060353 5.3696349292604296E-5 80-81 0.0 5.3696349292604296E-5 0.0 4.988390849282939 5.3696349292604296E-5 82-83 0.0 5.3696349292604296E-5 0.0 5.524253567048484 5.3696349292604296E-5 84-85 0.0 5.3696349292604296E-5 0.0 6.103100212422758 5.3696349292604296E-5 86-87 0.0 5.3696349292604296E-5 0.0 6.716258824995006 5.3696349292604296E-5 88-89 0.0 5.3696349292604296E-5 0.0 7.368105657232576 5.3696349292604296E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 6325 0.0 24.244753 12-13 TTTGCGT 675 0.0 22.43746 3 TTGCGTT 700 0.0 22.31225 4 GTACTTT 6910 0.0 22.191896 14-15 CTTTGCG 685 0.0 22.109903 2 AGTACTT 6495 0.0 21.132563 12-13 CGCACCG 60 0.004722819 19.822445 66-67 ACTTTTT 8215 0.0 19.330435 16-17 GTACATG 10890 0.0 19.198286 1 TACATGG 11175 0.0 18.592785 2 CATTCCG 410 1.8189894E-12 18.470356 9 CGTATAG 130 0.007649871 18.23385 1 ACATGGG 11210 0.0 17.690329 3 TACTTTT 8055 0.0 17.304028 14-15 CATGGGG 3585 0.0 15.578309 4 GTATCAA 31270 0.0 15.555044 1 GTACACT 965 0.0 15.203732 6 CATGGGA 7455 0.0 15.046247 4 AGAGTAC 19090 0.0 14.95032 10-11 TTAGGCA 770 0.0 14.751901 4 >>END_MODULE