FastQCFastQC Report
Thu 26 May 2016
SRR938918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862324
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT110350.5925392144438885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74890.40213195985231354No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT67400.36191339423215296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60100.3227150592485518No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48100.25827944009742665No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40780.2189737124152403No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA37640.20211305873736257No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC37110.19926715222485453No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC36070.1936827318984237No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32510.17456683155025657No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30840.16559954121839165No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30390.16318320550022444No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30230.16232406391154278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29030.15588050199643028No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28220.15153109770372933No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC27990.15029608166999942No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT24540.13177084116405094No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA22740.12210549829138216No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA18850.1012176184165591No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG909.003087E-426.3619882
ATCACGC909.003087E-426.3619883
GAGTACT70850.023.71095712-13
GTACTTT76300.022.04841214-15
CGGTCCG550.00289294421.57237434-35
TTTGCGT5150.020.2705763
GTACATG107150.020.1884061
AGTACTT72500.020.09490812-13
CATTCCG3102.6375346E-1019.8995849
TACATGG109600.019.352972
CTTTGCG5450.019.1547662
ACATGGG109150.018.6067583
ACTTTTT90500.018.58788516-17
CATGGGG36950.017.2084224
GTAAGCG1254.6103214E-617.0825794-95
GTATAGA5650.016.8663021
GTTCGCG850.001800497616.74806660-61
TGCGTAA1750.00212598316.3362181
TACTTTT90250.016.22159614-15
CATGGGA71500.016.0605354
Produced by FastQC (version 0.11.5)