##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938918_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1862324 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14959265949427 33.0 31.0 34.0 30.0 34.0 2 32.38497329143586 34.0 31.0 34.0 31.0 34.0 3 32.47129607952215 34.0 31.0 34.0 30.0 34.0 4 36.042606979236695 37.0 35.0 37.0 35.0 37.0 5 35.960870933306985 37.0 35.0 37.0 35.0 37.0 6 36.00682265814112 37.0 35.0 37.0 35.0 37.0 7 35.95623264265509 37.0 35.0 37.0 35.0 37.0 8 35.928396992145295 37.0 35.0 37.0 35.0 37.0 9 37.59345795898029 39.0 37.0 39.0 35.0 39.0 10-11 37.521827565987444 39.0 37.0 39.0 35.0 39.0 12-13 37.45578723143771 39.0 37.0 39.0 34.0 39.0 14-15 38.67720466470926 40.0 38.0 41.0 34.0 41.0 16-17 38.668844411606145 40.0 38.0 41.0 34.0 41.0 18-19 38.80496948973433 40.0 38.0 41.0 34.0 41.0 20-21 38.81533986567321 40.0 38.0 41.0 34.0 41.0 22-23 38.76126978978953 40.0 38.0 41.0 34.0 41.0 24-25 38.695275634100184 40.0 38.0 41.0 34.0 41.0 26-27 38.5320059774776 40.0 38.0 41.0 34.0 41.0 28-29 38.35915232795153 40.0 38.0 41.0 34.0 41.0 30-31 38.20376395299636 40.0 38.0 41.0 34.0 41.0 32-33 38.042071089670756 40.0 37.0 41.0 33.0 41.0 34-35 38.01664882157992 40.0 37.0 41.0 33.0 41.0 36-37 37.90756603040073 40.0 37.0 41.0 33.0 41.0 38-39 37.778554376145074 40.0 37.0 41.0 33.0 41.0 40-41 37.64509075756958 40.0 37.0 41.0 33.0 41.0 42-43 37.46976009545063 40.0 37.0 41.0 32.0 41.0 44-45 37.21028993880764 40.0 37.0 41.0 31.5 41.0 46-47 36.879537341515224 40.0 36.0 41.0 31.0 41.0 48-49 36.56925432953665 40.0 36.0 41.0 30.0 41.0 50-51 36.311789194576235 39.0 36.0 41.0 30.0 41.0 52-53 36.09365717243616 39.0 35.0 41.0 29.0 41.0 54-55 35.86614117629371 39.0 35.0 40.5 28.5 41.0 56-57 35.6062207757619 39.0 35.0 40.0 28.0 41.0 58-59 35.32118390784847 38.0 35.0 40.0 28.0 41.0 60-61 34.90873177814387 38.0 34.0 40.0 26.5 41.0 62-63 34.59806671664007 38.0 34.0 40.0 26.0 41.0 64-65 34.34409775098211 37.0 34.0 40.0 26.0 41.0 66-67 33.88878922249834 36.5 33.5 39.0 25.5 41.0 68-69 33.46576401313628 36.0 33.0 39.0 25.0 40.0 70-71 32.988628187146816 35.5 32.5 38.5 24.0 40.0 72-73 32.463161619567806 35.0 32.0 37.5 22.5 39.5 74-75 31.999994898846815 35.0 31.5 37.0 21.5 39.0 76-77 30.002212289590858 33.0 29.5 35.0 19.5 37.5 78-79 30.983906667153512 34.0 31.0 36.0 20.0 38.0 80-81 30.974395164321567 34.0 31.0 36.0 20.0 37.0 82-83 30.646325773603305 34.0 31.0 35.0 18.0 37.0 84-85 30.254729305963945 34.0 30.5 35.0 16.5 36.5 86-87 29.722494582038358 34.0 30.0 35.0 8.5 36.0 88-89 29.149594807348237 34.0 29.0 35.0 2.0 35.5 90-91 28.697112317727743 34.0 29.0 35.0 2.0 35.0 92-93 28.37024357737966 34.0 29.0 35.0 2.0 35.0 94-95 27.996021100517417 33.0 28.5 35.0 2.0 35.0 96-97 27.395248624836498 33.0 27.0 35.0 2.0 35.0 98-99 26.539719458053487 33.0 25.5 34.5 2.0 35.0 100-101 22.19270680075003 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21978817092050207 1101 2 0.15526720852168552 1101 3 0.13272402941012018 1101 4 0.06525127394733232 1101 5 0.17419963212898892 1101 6 0.16009822086120806 1101 7 0.17920864547256343 1101 8 0.1968150833713196 1101 9 0.2909108356533423 1101 10-11 0.322974442375596 1101 12-13 0.27559938523065597 1101 14-15 0.3828418258760067 1101 16-17 0.25202113660960634 1101 18-19 0.23453808151180766 1101 20-21 0.21798264605817508 1101 22-23 0.22542892539507164 1101 24-25 0.14855992346299018 1101 26-27 0.2664623109372286 1101 28-29 0.2869247772447707 1101 30-31 0.2594393994221349 1101 32-33 0.41900450948527634 1101 34-35 0.3651947599324714 1101 36-37 0.331405695262049 1101 38-39 0.41988882272020334 1101 40-41 0.43276741783193273 1101 42-43 0.4158305159911393 1101 44-45 0.3805758364104932 1101 46-47 0.4586119485482385 1101 48-49 0.017771821201385762 1101 50-51 0.1426955391764011 1101 52-53 0.3247729872017473 1101 54-55 0.404119046749571 1101 56-57 0.10090010326896248 1101 58-59 0.25098680251183225 1101 60-61 0.29389192931139263 1101 62-63 0.15928453749363314 1101 64-65 0.07710353910339762 1101 66-67 0.21764486148668993 1101 68-69 0.0957941938448954 1101 70-71 0.18745556924712758 1101 72-73 0.0029592911343812034 1101 74-75 0.02929306744194804 1101 76-77 -0.07458502410731782 1101 78-79 0.20247051262519022 1101 80-81 0.42194059778049464 1101 82-83 0.3883656759543186 1101 84-85 0.38351789729653873 1101 86-87 0.564117885261421 1101 88-89 0.5764053905496631 1101 90-91 0.5673306236526692 1101 92-93 0.6509293318910814 1101 94-95 0.5484912508711481 1101 96-97 0.5372504842691548 1101 98-99 0.44404229355572866 1101 100-101 0.9297373066407388 1102 1 0.1489021583776733 1102 2 0.1272989729997036 1102 3 0.1495236046370323 1102 4 0.06854192960729932 1102 5 0.12606168773685766 1102 6 0.0971419738249395 1102 7 0.15972384981112242 1102 8 0.13264863376637237 1102 9 0.23614716577416317 1102 10-11 0.25914043114182306 1102 12-13 0.24660745324778333 1102 14-15 0.20796338043864893 1102 16-17 0.22563778557739766 1102 18-19 0.15108469635081434 1102 20-21 0.28748530756879376 1102 22-23 0.3244304414876922 1102 24-25 0.34508342957744986 1102 26-27 0.20139906249172412 1102 28-29 0.27837722069184423 1102 30-31 0.18499507742276222 1102 32-33 0.2322736593380057 1102 34-35 0.36716267487099685 1102 36-37 0.3005246584417023 1102 38-39 0.23491133035076217 1102 40-41 0.3425974752030143 1102 42-43 0.3181555809426797 1102 44-45 0.49179007966655064 1102 46-47 0.4944982616380642 1102 48-49 0.40281443208652945 1102 50-51 0.39154427721296514 1102 52-53 0.329443153571205 1102 54-55 0.4106754794570975 1102 56-57 0.38341010480508686 1102 58-59 0.3390552537597671 1102 60-61 0.1577681840951044 1102 62-63 0.26764124670439315 1102 64-65 0.3263012455958787 1102 66-67 0.4132199748596861 1102 68-69 0.4961904149673373 1102 70-71 0.35862676749459865 1102 72-73 0.3509097275954076 1102 74-75 0.4228099348072121 1102 76-77 0.17800886343467326 1102 78-79 0.2472670423092147 1102 80-81 0.41568805147955956 1102 82-83 0.4618963350872747 1102 84-85 0.557627443337001 1102 86-87 0.5146022668358832 1102 88-89 0.5988108351254553 1102 90-91 0.6370533523522361 1102 92-93 0.7236042669395353 1102 94-95 0.7775945522129213 1102 96-97 0.6106532439584598 1102 98-99 0.5809290941995684 1102 100-101 1.0692784381667835 1103 1 0.06671825033169654 1103 2 0.050862191390507405 1103 3 0.18113280003932886 1103 4 0.17141549282569457 1103 5 0.16686628543801163 1103 6 0.06380864049160095 1103 7 0.08328706820192622 1103 8 0.11138426595028506 1103 9 0.10338854508450623 1103 10-11 0.12379560355561381 1103 12-13 0.07764193600639402 1103 14-15 0.1889978631972653 1103 16-17 0.06385617638199648 1103 18-19 0.21775136301748432 1103 20-21 0.1863358822814405 1103 22-23 0.07126952194746394 1103 24-25 0.10887653302572886 1103 26-27 0.14795078662965722 1103 28-29 0.26027377241598515 1103 30-31 0.2079835831698844 1103 32-33 0.1673311306023777 1103 34-35 0.1619902610778965 1103 36-37 -0.02964775535140518 1103 38-39 0.05416420391397736 1103 40-41 0.19317218784669166 1103 42-43 0.1394199487587784 1103 44-45 0.24236479231022656 1103 46-47 0.3812798708334668 1103 48-49 0.19706730564974606 1103 50-51 0.17229140364974427 1103 52-53 0.3170868317321265 1103 54-55 0.2951582380777893 1103 56-57 0.2290997599775011 1103 58-59 0.3189403112310316 1103 60-61 0.293975080646824 1103 62-63 0.29292860302624035 1103 64-65 0.2780253835269093 1103 66-67 0.28448434267578193 1103 68-69 0.2395812195650393 1103 70-71 0.1468451582992003 1103 72-73 0.2371166241471343 1103 74-75 0.30671798078422796 1103 76-77 0.10272150711283601 1103 78-79 0.3372095710448484 1103 80-81 0.44758460320369764 1103 82-83 0.47885035807577836 1103 84-85 0.2728573283944691 1103 86-87 0.21316548522669265 1103 88-89 0.47869589259672196 1103 90-91 0.5246970305131562 1103 92-93 0.3767651864797692 1103 94-95 0.2132267361209692 1103 96-97 0.16065324395846048 1103 98-99 0.11541185282025879 1103 100-101 0.6597956795460931 1104 1 -0.011853178239732642 1104 2 -0.01941304514088671 1104 3 -0.00935902887614759 1104 4 0.029453650617547567 1104 5 0.0062595139937968725 1104 6 0.029134310989689993 1104 7 -0.002521048148061311 1104 8 0.12028644751256223 1104 9 0.030067722502600702 1104 10-11 0.021952222525037257 1104 12-13 0.059786747954170494 1104 14-15 0.08122751367815084 1104 16-17 0.003706829286834079 1104 18-19 0.1686447088615708 1104 20-21 0.14377000535596096 1104 22-23 0.03975024757104251 1104 24-25 0.12647759643960654 1104 26-27 0.0861594034097024 1104 28-29 0.09155456059333034 1104 30-31 -0.05409515637190765 1104 32-33 0.10505886537444553 1104 34-35 -0.06636809375829245 1104 36-37 -0.025364777792560744 1104 38-39 0.11582461520493581 1104 40-41 0.041088365812136374 1104 42-43 -0.09664229222957488 1104 44-45 0.10903536858671004 1104 46-47 0.03891729361867391 1104 48-49 -0.10359146360507765 1104 50-51 -0.12452421915110534 1104 52-53 -0.2817754718099863 1104 54-55 -0.07357661218415501 1104 56-57 -0.02213372192100138 1104 58-59 -0.06192996791511973 1104 60-61 0.004574032871396128 1104 62-63 0.1239357185161083 1104 64-65 0.09666053873842628 1104 66-67 0.1470048681273397 1104 68-69 0.22243953530513494 1104 70-71 -0.04804302685991502 1104 72-73 0.05134369428553498 1104 74-75 -0.0956492879623525 1104 76-77 -0.0336769603201148 1104 78-79 0.0676595671352338 1104 80-81 0.2317682766730833 1104 82-83 0.21998531780679542 1104 84-85 0.1967079031071144 1104 86-87 -0.04192873636999295 1104 88-89 0.17817395968562622 1104 90-91 0.305713920027582 1104 92-93 0.2283114371639492 1104 94-95 0.36384836408249654 1104 96-97 0.34960859621430274 1104 98-99 0.3772345129187791 1104 100-101 0.895797321582215 1105 1 -0.018919358404190234 1105 2 0.04316053139319109 1105 3 0.06190082801867902 1105 4 0.06826302963474262 1105 5 0.01894080342609783 1105 6 0.1129457737572892 1105 7 0.029166751902550914 1105 8 0.016272993158025884 1105 9 0.012290687572395598 1105 10-11 -0.06224120868790095 1105 12-13 -0.03607474119081644 1105 14-15 0.03135348299979768 1105 16-17 0.07634205587422827 1105 18-19 0.04237258239113828 1105 20-21 0.12026706355365491 1105 22-23 -0.038035493784541075 1105 24-25 0.03629983553327776 1105 26-27 -0.05260908751434101 1105 28-29 -0.07437508166891149 1105 30-31 0.06946746504905121 1105 32-33 0.03414713958844118 1105 34-35 0.04203902070696586 1105 36-37 0.09457410096085539 1105 38-39 0.11541274399707646 1105 40-41 0.11178620815469031 1105 42-43 0.12433015190261898 1105 44-45 -0.06965582841771578 1105 46-47 0.11429664950686913 1105 48-49 -0.07802491642355847 1105 50-51 0.025589379869849438 1105 52-53 0.1785994332880918 1105 54-55 0.04401663092467345 1105 56-57 0.06610633997456006 1105 58-59 0.1255485493107642 1105 60-61 0.1498951633594956 1105 62-63 0.032140375540862465 1105 64-65 -0.05246422314115762 1105 66-67 0.07418817898638963 1105 68-69 0.08101158711307477 1105 70-71 0.11609260703458091 1105 72-73 0.05166617197177459 1105 74-75 0.1626122157877674 1105 76-77 0.04599328460554375 1105 78-79 0.23874208491006854 1105 80-81 0.26770305591515253 1105 82-83 0.37001235619942463 1105 84-85 0.08260462714931194 1105 86-87 0.024956188986415384 1105 88-89 0.24091451954088683 1105 90-91 0.4851366330328304 1105 92-93 0.32666067876693816 1105 94-95 0.3156580163165188 1105 96-97 0.279299175018469 1105 98-99 0.5240312660453519 1105 100-101 1.123478371792416 1106 1 0.008745627320223548 1106 2 -0.07196110839134207 1106 3 -0.06825931855176748 1106 4 0.04155786337182832 1106 5 0.03448464052240041 1106 6 0.05515478207120594 1106 7 0.038044234568801016 1106 8 0.041822986656519845 1106 9 -0.02357574746400104 1106 10-11 0.05846525541115 1106 12-13 0.06051313913956591 1106 14-15 0.11695689977221235 1106 16-17 0.07175448860213152 1106 18-19 0.052432815299113145 1106 20-21 0.020956099919573035 1106 22-23 0.1779750343410953 1106 24-25 0.14076224111507685 1106 26-27 0.13967294719199685 1106 28-29 0.13632624285113337 1106 30-31 0.23189156536081157 1106 32-33 0.21638326934986907 1106 34-35 0.0833254314505325 1106 36-37 0.02962482811268785 1106 38-39 0.1959204900328544 1106 40-41 0.13353698047811946 1106 42-43 0.20278345291124822 1106 44-45 0.039858955883268266 1106 46-47 0.25885778553870864 1106 48-49 0.12008228262296683 1106 50-51 0.11445911002962816 1106 52-53 0.14735938968178885 1106 54-55 0.08761585222299573 1106 56-57 0.147294741288178 1106 58-59 0.19659891533629548 1106 60-61 0.18425408099127338 1106 62-63 0.30591385568292395 1106 64-65 0.10773981321550963 1106 66-67 0.2637006030250717 1106 68-69 0.24300908387927933 1106 70-71 0.2974756837689867 1106 72-73 0.17844075952672256 1106 74-75 0.369493058284327 1106 76-77 0.19549741383206154 1106 78-79 0.24857784639662484 1106 80-81 0.3863008444171605 1106 82-83 0.41076508883470453 1106 84-85 0.3262861495000102 1106 86-87 0.321675824951388 1106 88-89 0.3227931281077119 1106 90-91 0.5649128826314929 1106 92-93 0.7577055847600924 1106 94-95 0.8337684075715934 1106 96-97 0.6740103695022341 1106 98-99 0.8641405961067719 1106 100-101 1.3041729359789187 1107 1 -0.09506857777324029 1107 2 0.014438947316342876 1107 3 -0.03814173990588898 1107 4 -0.051144429744667264 1107 5 -0.04206089654061884 1107 6 0.0054995039206460206 1107 7 -0.10349390839837724 1107 8 -0.008131292726076822 1107 9 -0.15837582589455934 1107 10-11 -0.035433958878364535 1107 12-13 -0.06726804571160727 1107 14-15 0.06499008860354394 1107 16-17 0.09600703133426691 1107 18-19 0.027544093234659783 1107 20-21 -0.05603106715523154 1107 22-23 -0.07214911683441017 1107 24-25 -0.0771508646678285 1107 26-27 -0.17788392259800645 1107 28-29 -0.19300987057264507 1107 30-31 -0.2062553416714863 1107 32-33 0.03977845124585144 1107 34-35 0.05324969465224427 1107 36-37 0.04690352596996661 1107 38-39 0.031042536899384743 1107 40-41 -0.1292053891875753 1107 42-43 -6.96418889347683E-5 1107 44-45 0.13454354470618313 1107 46-47 0.06310604635329753 1107 48-49 0.11725673625090138 1107 50-51 0.0329047678537151 1107 52-53 0.14160827354286454 1107 54-55 0.1293511099962643 1107 56-57 0.21047837446772633 1107 58-59 0.040531572562386486 1107 60-61 -0.02229178591988301 1107 62-63 0.0199978215815122 1107 64-65 -0.09016219989656804 1107 66-67 0.16363353390295288 1107 68-69 0.08910835473162848 1107 70-71 -0.06930262224091166 1107 72-73 -0.0880339334854483 1107 74-75 -0.026001735307090712 1107 76-77 -0.22725764887878697 1107 78-79 0.009014333220719095 1107 80-81 0.15620687434660496 1107 82-83 0.22663469375603995 1107 84-85 0.08415974680551841 1107 86-87 0.17218818906464506 1107 88-89 0.36074062972183185 1107 90-91 0.5300506828009368 1107 92-93 0.40970235713494674 1107 94-95 0.2785962665456303 1107 96-97 0.41458434802667554 1107 98-99 0.22492956231993944 1107 100-101 0.5421826506175194 1108 1 -0.029240222045018527 1108 2 -0.06398764175742855 1108 3 0.011092013980743332 1108 4 -0.1211873992766428 1108 5 -0.05449077796577484 1108 6 -0.06479721786953974 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-0.7308739307245133 2316 60-61 -0.5839720275932407 2316 62-63 -0.7393061041584303 2316 64-65 -0.4417476125607891 2316 66-67 -0.6887156997832022 2316 68-69 -0.7396047811021731 2316 70-71 -0.7954553603069314 2316 72-73 -0.8224267901615434 2316 74-75 -1.0097454893276847 2316 76-77 -1.0781287339935268 2316 78-79 -1.1297838781408522 2316 80-81 -1.0630574372536508 2316 82-83 -1.2511711746934573 2316 84-85 -1.3696949113168095 2316 86-87 -1.370472642938779 2316 88-89 -1.7448354815578924 2316 90-91 -1.8468918452336673 2316 92-93 -2.1324855641343703 2316 94-95 -2.2279569596301023 2316 96-97 -2.544576034542132 2316 98-99 -2.8520342394370424 2316 100-101 -3.483254695002781 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 9.0 5 41.0 6 82.0 7 234.0 8 502.0 9 983.0 10 1668.0 11 2561.0 12 3705.0 13 5057.0 14 6436.0 15 9058.0 16 12367.0 17 17827.0 18 16970.0 19 10793.0 20 9371.0 21 9878.0 22 10582.0 23 11806.0 24 13833.0 25 16437.0 26 20095.0 27 24121.0 28 29177.0 29 36274.0 30 43867.0 31 53688.0 32 66933.0 33 85465.0 34 114871.0 35 163306.0 36 259205.0 37 431379.0 38 350267.0 39 23467.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.4626019604661 21.057622995498615 16.53957945031252 20.940195593722756 2 15.106393946488367 22.478043562774257 38.792766457394094 23.622796033343285 3 19.315758160234203 26.42569177006794 31.251006806549235 23.007543263148627 4 13.01492114154143 18.755705237112338 38.54624651779175 29.683127103554487 5 13.222789914702936 38.509059960318204 33.93056491517278 14.337585209806075 6 29.188100459425964 37.6135946269285 17.926150336890895 15.272154576754634 7 26.2212160719617 34.6499320204218 21.51258320249323 17.616268705123275 8 25.663418395510124 33.38634952886823 20.55243878079217 20.397793294829473 9 26.171869126961795 17.67243508648334 21.68784808658429 34.46784769997057 10-11 24.32049954787674 27.252320219252933 27.350047574965476 21.077132657904855 12-13 26.48201924047588 25.062556246925883 27.401810855683546 21.053613656914692 14-15 23.266488445851667 26.097267540372865 27.3539954566848 23.282248557090668 16-17 21.11489561412317 29.44879724938288 27.71400863353547 21.72229850295848 18-19 21.103766047153986 28.493377092278248 29.83098000133167 20.57187685923609 20-21 22.749800786544125 26.727465253092376 29.308138648269583 21.21459531209392 22-23 22.02347711783771 26.811500039735296 29.4009259398472 21.764096902579787 24-25 22.254049241700155 27.12062992261282 29.438298598954855 21.18702223673217 26-27 22.117526273623707 26.670520274667563 30.11667142774297 21.095282023965755 28-29 21.28380990633209 27.763831642614285 29.710351152645835 21.242007298407795 30-31 21.797845058110187 27.065779101810428 29.502707369931336 21.633668470148052 32-33 21.696734832392213 27.197227764878722 29.46681136042923 21.639226042299835 34-35 22.403687307041018 26.947310031974236 29.25931405070289 21.38968861028186 36-37 22.31779131747878 26.684505992445406 29.782571805702784 21.21513088437303 38-39 21.96291833941569 27.05085899930696 29.642558225897442 21.343664435379907 40-41 21.971770027643593 27.1280948688594 29.540841422001897 21.359293681495117 42-43 21.624276705514436 27.30664893959766 28.827312501879383 22.24176185300852 44-45 22.067910101137564 26.92374813068681 28.76884415364748 22.239497614528155 46-47 22.500859143433996 27.331205163452037 28.331033334765237 21.836902358348727 48-49 22.277061184118853 27.237858769283392 28.42930181496589 22.055778231631866 50-51 21.767465886562753 27.55440000515487 28.775164902197048 21.902969206085327 52-53 22.1273795537189 27.983798737491433 27.827971931844296 22.060849776945364 54-55 21.595221884054546 27.25519297394009 28.37639959534431 22.773185546661054 56-57 22.309463874170124 27.599467117429622 27.690213947734122 22.400855060666135 58-59 22.745202484215906 27.232271824683004 27.91378808560085 22.10873760550024 60-61 22.275594857575967 27.340640351699147 28.53136233748695 21.852402453237936 62-63 22.062380122900205 27.334475633670618 28.475684145186335 22.12746009824284 64-65 21.77956622898655 27.70875988485324 28.239469177944272 22.27220470821594 66-67 21.915873648306874 27.398600865384303 27.747740152736355 22.937785333572467 68-69 21.650526648146524 27.33380533121337 28.245341168820325 22.77032685181978 70-71 22.20480968939884 27.412496429192775 27.727962481286824 22.65473140012157 72-73 21.967794003621282 26.854886689963724 28.39457580957986 22.78274349683514 74-75 21.57857601577384 27.972764137711803 27.670077816749394 22.77858202976496 76-77 21.793080014699008 27.065206550897198 27.90757689761152 23.234136536792278 78-79 21.7388175950144 27.074062877384858 28.237663391366592 22.949456136234154 80-81 21.932864528406444 27.38060079771297 27.768100502383042 22.918434171497548 82-83 21.579086131092122 27.25014551710658 28.277839946217735 22.89292840558356 84-85 21.438267455072264 27.21062500402723 27.91079855062814 23.440308990272367 86-87 21.45240184817119 27.358908090481233 27.970447975013553 23.218242086334033 88-89 21.59765595503921 26.874750547447224 27.820890370322427 23.706703127191137 90-91 21.408581989030054 26.66410737184668 28.188315606849212 23.738995032274055 92-93 21.663174614084337 27.1741383346829 28.05457052585909 23.108116525373674 94-95 21.269285043848438 26.883667933184558 28.02914530446904 23.817901718497964 96-97 20.70077315082925 26.85282212000175 28.32848587067329 24.117918858495706 98-99 20.86895191169743 26.929873641750845 27.672333063419686 24.528841383132043 100-101 20.996305415743425 26.1441304007554 28.5822445706848 24.277319612816374 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 330.0 1 214.5 2 106.5 3 135.5 4 132.0 5 137.5 6 253.5 7 473.0 8 879.5 9 1258.5 10 1324.0 11 1329.0 12 1471.0 13 1626.0 14 1757.5 15 1920.5 16 2083.0 17 2278.0 18 2550.0 19 2815.5 20 3093.0 21 3511.5 22 4090.0 23 4777.0 24 5590.5 25 6894.5 26 9093.5 27 11528.5 28 13708.0 29 16404.5 30 19832.0 31 23900.0 32 28532.0 33 33375.5 34 38990.5 35 43944.5 36 49266.5 37 55710.5 38 58486.0 39 58264.0 40 59207.5 41 61021.0 42 62650.5 43 65383.0 44 67965.5 45 69069.0 46 70767.0 47 82448.5 48 110957.5 49 109832.5 50 81572.5 51 67843.5 52 60177.0 53 55899.0 54 51695.0 55 46016.5 56 40712.0 57 35979.5 58 31567.0 59 26741.5 60 22486.0 61 18417.0 62 14298.5 63 10634.5 64 7583.5 65 5653.5 66 4050.5 67 2965.5 68 2166.5 69 1407.0 70 967.5 71 680.0 72 483.0 73 389.0 74 269.0 75 194.0 76 142.0 77 64.5 78 26.5 79 15.5 80 7.5 81 5.0 82 3.0 83 1.0 84 1.0 85 1.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4059980970013811 2 0.0 3 0.0 4 0.0 5 4.8326714363343865E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001476649605546618 16-17 0.002738513813922819 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002335791194228287 36-37 0.007839666996720226 38-39 0.01312875740204175 40-41 0.002577424766045006 42-43 6.443561915112515E-4 44-45 4.8326714363343865E-4 46-47 2.1478539717041718E-4 48-49 1.3424087323151074E-4 50-51 4.2957079434083437E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.2217809575562576E-4 60-61 0.002711665639276517 62-63 0.0 64-65 2.684817464630215E-4 66-67 0.0016108904787781287 68-69 0.001476649605546618 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12535412742358473 78-79 5.3696349292604296E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14798713865041743 88-89 0.44374663055408187 90-91 0.34064963991228164 92-93 0.0 94-95 0.0 96-97 5.101153182797408E-4 98-99 0.0 100-101 1.8793722252411505E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1862324.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.346522197318 #Duplication Level Percentage of deduplicated Percentage of total 1 72.7614010556132 36.632834933543876 2 15.040824316777526 15.145063906612 3 5.224519329022915 7.891091351069073 4 2.342093379545912 4.71665025285999 5 1.353758530890037 3.407851696263192 6 0.7530436166093708 2.274787629550339 7 0.4749812847494226 1.6739559057163196 8 0.3430123069698063 1.3815581381446884 9 0.2530978453975992 1.146833666226323 >10 1.2992958144568685 11.860635550522064 >50 0.10112468703759372 3.496359043408897 >100 0.046643844189346036 4.150003756784339 >500 0.002460202431536012 0.8290718636036506 >1k 0.003315925016418103 3.70909357153867 >5k 3.208959693307841E-4 1.0899373463770239 >10k+ 1.0696532311026138E-4 0.5942713877795572 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11035 0.5925392144438885 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7489 0.40213195985231354 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6740 0.36191339423215296 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6010 0.3227150592485518 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4810 0.25827944009742665 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4078 0.2189737124152403 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3764 0.20211305873736257 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3711 0.19926715222485453 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3607 0.1936827318984237 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3251 0.17456683155025657 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3084 0.16559954121839165 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3039 0.16318320550022444 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3023 0.16232406391154278 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2903 0.15588050199643028 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2822 0.15153109770372933 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2799 0.15029608166999942 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2454 0.13177084116405094 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2274 0.12210549829138216 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1885 0.1012176184165591 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.3696349292604296E-5 0.0 10-11 0.0 0.0 0.0 5.3696349292604296E-5 0.0 12-13 0.0 0.0 0.0 1.6108904787781288E-4 0.0 14-15 0.0 0.0 0.0 1.8793722252411502E-4 0.0 16-17 0.0 0.0 0.0 2.1478539717041718E-4 0.0 18-19 0.0 0.0 0.0 2.1478539717041718E-4 0.0 20-21 0.0 0.0 2.6848174646302148E-5 2.684817464630215E-4 0.0 22-23 0.0 0.0 5.3696349292604296E-5 3.7587444504823004E-4 0.0 24-25 0.0 0.0 5.3696349292604296E-5 7.517488900964601E-4 0.0 26-27 0.0 0.0 5.3696349292604296E-5 0.001476649605546618 0.0 28-29 0.0 0.0 5.3696349292604296E-5 0.00437625246734725 0.0 30-31 0.0 0.0 5.3696349292604296E-5 0.011866893193665549 0.0 32-33 0.0 0.0 5.3696349292604296E-5 0.025559462263279646 0.0 34-35 0.0 0.0 5.3696349292604296E-5 0.04585668229588406 0.0 36-37 0.0 0.0 5.3696349292604296E-5 0.07611457512226659 0.0 38-39 0.0 0.0 5.3696349292604296E-5 0.12680392885448505 0.0 40-41 0.0 0.0 5.3696349292604296E-5 0.18992398744794137 0.0 42-43 0.0 0.0 5.3696349292604296E-5 0.26042729406913084 0.0 44-45 0.0 0.0 5.3696349292604296E-5 0.34567024857114015 0.0 46-47 0.0 0.0 5.3696349292604296E-5 0.4440419604751912 0.0 48-49 0.0 0.0 5.3696349292604296E-5 0.5515957481082776 0.0 50-51 0.0 0.0 5.3696349292604296E-5 0.6676335589295955 0.0 52-53 0.0 0.0 5.3696349292604296E-5 0.7880744703929069 0.0 54-55 0.0 0.0 5.3696349292604296E-5 0.9230402443398678 0.0 56-57 0.0 0.0 5.3696349292604296E-5 1.0882639111132113 0.0 58-59 0.0 0.0 5.3696349292604296E-5 1.270052901643323 0.0 60-61 0.0 0.0 5.3696349292604296E-5 1.4769717836423737 0.0 62-63 0.0 0.0 5.3696349292604296E-5 1.696106585105492 0.0 64-65 0.0 0.0 5.3696349292604296E-5 1.9310281132606355 0.0 66-67 0.0 0.0 5.3696349292604296E-5 2.198731262658914 0.0 68-69 0.0 0.0 5.3696349292604296E-5 2.507834297361791 0.0 70-71 0.0 0.0 5.3696349292604296E-5 2.8536387868061626 0.0 72-73 0.0 0.0 5.3696349292604296E-5 3.2084374147570456 0.0 74-75 0.0 0.0 5.3696349292604296E-5 3.603400911978796 0.0 76-77 0.0 0.0 5.3696349292604296E-5 4.013050360732074 0.0 78-79 0.0 0.0 5.3696349292604296E-5 4.4916190738024095 0.0 80-81 0.0 0.0 5.3696349292604296E-5 5.018460804886797 0.0 82-83 0.0 0.0 5.3696349292604296E-5 5.557035188291619 0.0 84-85 0.0 0.0 5.3696349292604296E-5 6.137439027795378 0.0 86-87 0.0 0.0 5.3696349292604296E-5 6.75057079219298 0.0 88-89 0.0 0.0 5.3696349292604296E-5 7.4031693733206465 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 90 9.003087E-4 26.361988 2 ATCACGC 90 9.003087E-4 26.361988 3 GAGTACT 7085 0.0 23.710957 12-13 GTACTTT 7630 0.0 22.048412 14-15 CGGTCCG 55 0.002892944 21.572374 34-35 TTTGCGT 515 0.0 20.270576 3 GTACATG 10715 0.0 20.188406 1 AGTACTT 7250 0.0 20.094908 12-13 CATTCCG 310 2.6375346E-10 19.899584 9 TACATGG 10960 0.0 19.35297 2 CTTTGCG 545 0.0 19.154766 2 ACATGGG 10915 0.0 18.606758 3 ACTTTTT 9050 0.0 18.587885 16-17 CATGGGG 3695 0.0 17.208422 4 GTAAGCG 125 4.6103214E-6 17.08257 94-95 GTATAGA 565 0.0 16.866302 1 GTTCGCG 85 0.0018004976 16.748066 60-61 TGCGTAA 175 0.002125983 16.336218 1 TACTTTT 9025 0.0 16.221596 14-15 CATGGGA 7150 0.0 16.060535 4 >>END_MODULE