FastQCFastQC Report
Thu 26 May 2016
SRR938917_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938917_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135812
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87680.41052302356199893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73830.34567649212571144No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44960.21050541901628048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38060.17819920479892423No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29410.13769938552644145No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25420.11901796600075289No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23780.1113393875490914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT72350.023.29285812-13
GTACTTT76450.021.8274614-15
GTACATG98900.021.0673751
GGTATCA149650.020.9953671
GTATCAA192500.020.8337861
TACATGG99050.020.6066362
ACATGGG99750.019.9397963
GTGTAGC12650.019.1347081
AGTACTT76700.018.63901312-13
TATCAAC214950.018.6387712
ATCAACG216750.018.396593
CAACGCA217850.018.30375
ACTTTTT92600.018.2779416-17
TCAACGC218400.018.2576054
GCGTAGC1300.007600569618.2544561
AACGCAG221100.018.0912726
GACCGGT1350.00959085317.532247
GTAGCCC12850.017.3212433
CATGGGG50150.017.2808254
ACGCAGA236300.016.7272267
Produced by FastQC (version 0.11.5)