##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938917_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2135812 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.624940303734597 33.0 31.0 34.0 30.0 34.0 2 31.717861871737774 33.0 31.0 34.0 30.0 34.0 3 31.747350422228173 34.0 31.0 34.0 28.0 34.0 4 35.28309467312666 37.0 35.0 37.0 33.0 37.0 5 35.325542229372246 37.0 35.0 37.0 33.0 37.0 6 35.40104185199821 37.0 35.0 37.0 33.0 37.0 7 35.32588589257856 37.0 35.0 37.0 33.0 37.0 8 35.31043603088661 37.0 35.0 37.0 33.0 37.0 9 36.87754025167009 39.0 37.0 39.0 33.0 39.0 10-11 36.759708485578315 39.0 37.0 39.0 32.0 39.0 12-13 36.634710826608334 39.0 37.0 39.0 32.0 39.0 14-15 37.77936353948756 40.0 38.0 41.0 32.0 41.0 16-17 37.68289507690752 40.0 38.0 41.0 32.0 41.0 18-19 37.6090067852414 40.0 37.0 41.0 32.0 41.0 20-21 37.74404956990597 40.0 38.0 41.0 32.0 41.0 22-23 37.6802614181398 40.0 38.0 41.0 32.0 41.0 24-25 37.582845540712384 40.0 37.5 41.0 32.0 41.0 26-27 37.44707656853693 40.0 37.0 41.0 31.5 41.0 28-29 37.30665737433819 40.0 37.0 41.0 31.0 41.0 30-31 37.10431231775081 40.0 37.0 41.0 30.5 41.0 32-33 36.88523615374387 40.0 36.0 41.0 30.0 41.0 34-35 36.54665110974187 39.5 36.0 41.0 30.0 41.0 36-37 36.27394897116413 39.0 35.0 41.0 30.0 41.0 38-39 36.023237766245344 39.0 35.0 41.0 28.5 41.0 40-41 35.864257247360726 39.0 35.0 41.0 27.5 41.0 42-43 35.63968411077379 39.0 35.0 40.5 27.0 41.0 44-45 35.53841911179448 39.0 35.0 41.0 26.5 41.0 46-47 35.29654693390617 39.0 35.0 41.0 25.5 41.0 48-49 35.024327281614674 39.0 35.0 41.0 24.0 41.0 50-51 33.91975791876813 38.0 33.0 40.0 23.0 40.5 52-53 34.02216253115911 38.0 33.5 39.5 23.0 40.5 54-55 34.49565738932078 38.0 34.0 40.0 23.0 41.0 56-57 34.38627159131984 38.0 34.0 40.0 22.5 41.0 58-59 34.16203322202516 38.0 34.0 40.0 22.0 41.0 60-61 33.83494661515152 38.0 33.5 40.0 20.5 41.0 62-63 33.44631994763584 37.0 33.0 40.0 19.5 41.0 64-65 33.04775326667328 37.0 33.0 40.0 18.0 41.0 66-67 32.621173586439255 36.0 32.0 39.0 14.0 41.0 68-69 32.169563145070825 36.0 31.5 39.0 11.5 40.5 70-71 31.714156255325843 35.0 31.0 38.5 8.0 40.0 72-73 31.257068506029558 35.0 31.0 37.5 7.0 40.0 74-75 30.779509853863544 35.0 30.5 37.0 3.5 39.0 76-77 30.33541107550665 35.0 30.0 36.5 2.0 39.0 78-79 29.913775650665883 34.5 29.5 36.0 2.0 38.5 80-81 29.445747331693987 34.0 29.0 35.5 2.0 37.5 82-83 28.96575190138458 34.0 29.0 35.0 2.0 37.0 84-85 28.53568502283909 34.0 29.0 35.0 2.0 36.5 86-87 28.077683101321654 34.0 27.5 35.0 2.0 36.0 88-89 27.577207872228456 34.0 26.5 35.0 2.0 36.0 90-91 27.095510513097594 33.0 25.5 35.0 2.0 35.0 92-93 26.574720059630714 33.0 25.0 35.0 2.0 35.0 94-95 26.014979548761783 33.0 23.5 35.0 2.0 35.0 96-97 25.332694310173366 32.0 19.5 35.0 2.0 35.0 98-99 24.41949197775834 32.0 6.5 34.5 2.0 35.0 100-101 21.14345738295318 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1877029787204627 1101 2 0.345050019032616 1101 3 0.39523870893274804 1101 4 0.27019348189656256 1101 5 0.14153528075024013 1101 6 0.3374508846559081 1101 7 0.3660282628082143 1101 8 0.3800552876296166 1101 9 0.4679414665815571 1101 10-11 0.39594231299674476 1101 12-13 0.4169156954462707 1101 14-15 0.5063826436810857 1101 16-17 0.520248790787889 1101 18-19 0.6293986091305257 1101 20-21 0.5843626586714379 1101 22-23 0.6823577293890466 1101 24-25 0.5142850106980958 1101 26-27 0.6208299907744816 1101 28-29 0.6782872577489485 1101 30-31 0.5261256088252892 1101 32-33 0.5299964550012177 1101 34-35 0.5631951682980585 1101 36-37 0.4254386093450293 1101 38-39 0.5821578957749622 1101 40-41 0.3577752058056092 1101 42-43 0.46909486678828927 1101 44-45 0.5059607363726357 1101 46-47 0.4170314304023748 1101 48-49 0.5478572307280771 1101 50-51 0.5026666005154254 1101 52-53 0.5246543800840655 1101 54-55 0.5349999855527372 1101 56-57 0.5138842327469106 1101 58-59 0.5090109909565399 1101 60-61 0.5492723950376188 1101 62-63 0.5469544567207407 1101 64-65 0.3906979785880651 1101 66-67 0.4096370039011248 1101 68-69 0.31823196682519495 1101 70-71 0.33660058041328966 1101 72-73 0.24709116026166456 1101 74-75 0.19114659248812416 1101 76-77 0.23076212571489307 1101 78-79 0.14479796659187727 1101 80-81 0.1529617553343634 1101 82-83 0.16658371987168863 1101 84-85 0.17716481075847312 1101 86-87 0.2293664239386075 1101 88-89 0.21031090682522802 1101 90-91 0.1805627114951207 1101 92-93 0.04891370954374352 1101 94-95 -0.09601541628830645 1101 96-97 -0.21110014186639958 1101 98-99 -0.20400778981880663 1101 100-101 0.17191444946483614 1102 1 0.1383292170315933 1102 2 0.18976053273540572 1102 3 0.33702832409789707 1102 4 0.1638990127888107 1102 5 0.248639883843218 1102 6 0.24065721383078653 1102 7 0.29252787770141 1102 8 0.38968208457640685 1102 9 0.43779210701654847 1102 10-11 0.44574733942028644 1102 12-13 0.33199920761038726 1102 14-15 0.38208670799878064 1102 16-17 0.5350352954077948 1102 18-19 0.4179890683120746 1102 20-21 0.4111715067217858 1102 22-23 0.4695627022693927 1102 24-25 0.5495965729592811 1102 26-27 0.5090089208537023 1102 28-29 0.6807040618290117 1102 30-31 0.6309433985392516 1102 32-33 0.46525360080961775 1102 34-35 0.4453829242718328 1102 36-37 0.5113579716969738 1102 38-39 0.5966920288130879 1102 40-41 0.39540327222012195 1102 42-43 0.3151536909487973 1102 44-45 0.5365048085845885 1102 46-47 0.4822182739462164 1102 48-49 0.3977548973575651 1102 50-51 0.30430927379290296 1102 52-53 0.2660209141901504 1102 54-55 0.39246593035934296 1102 56-57 0.393498122939441 1102 58-59 0.3200675109632556 1102 60-61 0.32636042369246354 1102 62-63 0.27177087916207654 1102 64-65 0.22961916555037476 1102 66-67 0.3108400790669421 1102 68-69 0.31462728226970427 1102 70-71 0.2599776739559445 1102 72-73 0.28591970701802083 1102 74-75 0.3216833293323056 1102 76-77 0.5402981786732539 1102 78-79 0.3242543709293244 1102 80-81 0.21270782685819256 1102 82-83 0.23127904387413523 1102 84-85 0.16819058709113577 1102 86-87 0.1524650729085728 1102 88-89 0.14207414710168464 1102 90-91 0.37541078829392305 1102 92-93 0.3809776998989811 1102 94-95 0.07383115725911082 1102 96-97 -0.05520199469546938 1102 98-99 0.1112331333497032 1102 100-101 0.3219843475356541 1103 1 0.3556753165020794 1103 2 0.33718372575787114 1103 3 0.40057545201868905 1103 4 0.3678816936373934 1103 5 0.15124001898568906 1103 6 0.41503851469774844 1103 7 0.28311139353741055 1103 8 0.15908996145196141 1103 9 0.38846736689431793 1103 10-11 0.449125524167286 1103 12-13 0.578503376514341 1103 14-15 0.547758911082525 1103 16-17 0.49981353706747456 1103 18-19 0.5712774848726596 1103 20-21 0.583336506229081 1103 22-23 0.4981571753042502 1103 24-25 0.5114114086666532 1103 26-27 0.5587265696159989 1103 28-29 0.6812940532418352 1103 30-31 0.6238824808575103 1103 32-33 0.5169757623651989 1103 34-35 0.4969770071632382 1103 36-37 0.4283861243677549 1103 38-39 0.6729144196282206 1103 40-41 0.6250768488501279 1103 42-43 0.4773123405698172 1103 44-45 0.632715138135147 1103 46-47 0.6452027104403157 1103 48-49 0.5839357750998815 1103 50-51 0.47044804017541253 1103 52-53 0.4581450692983182 1103 54-55 0.6443319321733085 1103 56-57 0.6737152608953707 1103 58-59 0.7031725399927495 1103 60-61 0.7410480927462402 1103 62-63 0.5932333690816947 1103 64-65 0.5813297132997093 1103 66-67 0.6724652226055454 1103 68-69 0.568207954482844 1103 70-71 0.5085263252461303 1103 72-73 0.4510625015310765 1103 74-75 0.426976308962395 1103 76-77 0.45049869531166564 1103 78-79 0.3610688798412909 1103 80-81 0.3519925571665077 1103 82-83 0.5780018137719551 1103 84-85 0.4165562329494783 1103 86-87 0.5409336945156831 1103 88-89 0.464501060564249 1103 90-91 0.5370724575045678 1103 92-93 0.4823713817872779 1103 94-95 0.570161998903103 1103 96-97 0.6622647676971134 1103 98-99 0.7582433072450847 1103 100-101 0.7605361260065777 1104 1 0.15301899684815723 1104 2 0.23844106971808188 1104 3 0.3447903404424686 1104 4 0.37166387062808326 1104 5 0.2246723745902912 1104 6 0.34912803354966115 1104 7 0.22145686102208373 1104 8 0.41965461928688086 1104 9 0.4008641416488814 1104 10-11 0.29893279206416423 1104 12-13 0.3354401579856656 1104 14-15 0.4480669626016933 1104 16-17 0.42940472411531516 1104 18-19 0.5026872953440105 1104 20-21 0.397091651249184 1104 22-23 0.4290925894346387 1104 24-25 0.49297916800665575 1104 26-27 0.5131950158249339 1104 28-29 0.525442423727263 1104 30-31 0.48064814480748197 1104 32-33 0.45735250077729006 1104 34-35 0.5918931325907835 1104 36-37 0.5205027241666471 1104 38-39 0.46428585359705465 1104 40-41 0.46876144287580956 1104 42-43 0.5212024355440334 1104 44-45 0.5372204165909267 1104 46-47 0.6156199693215427 1104 48-49 0.5161254596719189 1104 50-51 0.30257895307538973 1104 52-53 0.522562894906379 1104 54-55 0.7175923706419596 1104 56-57 0.6174592512919972 1104 58-59 0.6947073702045827 1104 60-61 0.6911992448909103 1104 62-63 0.8251567070327965 1104 64-65 0.7142593911949646 1104 66-67 0.6557094239005181 1104 68-69 0.7846031519976435 1104 70-71 0.5759252139817619 1104 72-73 0.7231448578353401 1104 74-75 0.7192519321120372 1104 76-77 0.6895830816374549 1104 78-79 0.49911609108546884 1104 80-81 0.6110192063216111 1104 82-83 0.9123083030441244 1104 84-85 0.8511241462152839 1104 86-87 0.6409216253330321 1104 88-89 0.7079981949454783 1104 90-91 0.7081275089697634 1104 92-93 0.518636776866849 1104 94-95 0.6790285252374098 1104 96-97 0.7108183648632824 1104 98-99 0.9627382406871661 1104 100-101 1.153627780828952 1105 1 0.21182878489474533 1105 2 0.19884472451364843 1105 3 0.2889680751938464 1105 4 0.28250587426517626 1105 5 0.23853627991146453 1105 6 0.28440166085414376 1105 7 0.32119388299257423 1105 8 0.30378983392396464 1105 9 0.2975650917715953 1105 10-11 0.4255914410003996 1105 12-13 0.31652178654886853 1105 14-15 0.3426115939260299 1105 16-17 0.4239812082354746 1105 18-19 0.45349048379924994 1105 20-21 0.5012060268441658 1105 22-23 0.33721531700590646 1105 24-25 0.3473412808863259 1105 26-27 0.38936618947775514 1105 28-29 0.3914223867789772 1105 30-31 0.3640710019792124 1105 32-33 0.39892210743615664 1105 34-35 0.5945052302537661 1105 36-37 0.4305125614840293 1105 38-39 0.6015136643596932 1105 40-41 0.518534924749666 1105 42-43 0.37687991973131574 1105 44-45 0.5788566398597794 1105 46-47 0.5216209013285678 1105 48-49 0.5624908848574179 1105 50-51 0.5370993724256294 1105 52-53 0.5569136331622744 1105 54-55 0.6472141716192468 1105 56-57 0.5743233724134598 1105 58-59 0.5969688465335565 1105 60-61 0.7687321736809025 1105 62-63 0.6253294916095058 1105 64-65 0.6319165458886431 1105 66-67 0.5476960880683635 1105 68-69 0.5522247369340647 1105 70-71 0.4726077597322629 1105 72-73 0.490758744210833 1105 74-75 0.46198876089676233 1105 76-77 0.5195056317087783 1105 78-79 0.40897316008454254 1105 80-81 0.645402543171425 1105 82-83 0.87247125752085 1105 84-85 0.8093607764011956 1105 86-87 0.6635116088797623 1105 88-89 0.8490091266614073 1105 90-91 0.8017362358659597 1105 92-93 0.7859281657137025 1105 94-95 0.7360105065149902 1105 96-97 0.8919267699836944 1105 98-99 1.0150719752277944 1105 100-101 1.017068902836467 1106 1 0.11520815430178999 1106 2 0.18513810342252413 1106 3 0.2193713284986245 1106 4 0.1712124372084034 1106 5 0.17873256858737108 1106 6 0.13644434978013464 1106 7 0.19602821964839734 1106 8 0.1614337929859886 1106 9 0.15950966892244622 1106 10-11 0.11782848809228597 1106 12-13 0.2693966415861766 1106 14-15 0.18983056632718132 1106 16-17 0.2173356949965921 1106 18-19 0.38132953136469183 1106 20-21 0.26755581851189447 1106 22-23 0.39229846616547803 1106 24-25 0.38037160943121506 1106 26-27 0.32656983874642265 1106 28-29 0.3030143318538805 1106 30-31 0.2660534264299841 1106 32-33 0.34908668922954433 1106 34-35 0.28948220601446195 1106 36-37 0.31748631724706655 1106 38-39 0.3437916243083521 1106 40-41 0.35716545350938134 1106 42-43 0.305372898489658 1106 44-45 0.43542404885018726 1106 46-47 0.3364180047262195 1106 48-49 0.2830140902855547 1106 50-51 0.2696776825176386 1106 52-53 0.23968629676834752 1106 54-55 0.19881019169841352 1106 56-57 0.25446100614779965 1106 58-59 0.26263126034093887 1106 60-61 0.3838017348410858 1106 62-63 0.44697630038341885 1106 64-65 0.357607587066191 1106 66-67 0.2371211031054088 1106 68-69 0.3763935360777495 1106 70-71 0.4865106881066197 1106 72-73 0.40693797648188834 1106 74-75 0.4239227086129702 1106 76-77 0.39937673876555735 1106 78-79 0.48661292319468075 1106 80-81 0.42734980908080544 1106 82-83 0.34461760320495216 1106 84-85 0.42942314815602245 1106 86-87 0.6120233949922778 1106 88-89 0.6951802740495658 1106 90-91 0.8251284350238883 1106 92-93 0.7870822850731827 1106 94-95 0.7794640772840005 1106 96-97 0.9570985783970833 1106 98-99 0.9319371200323268 1106 100-101 0.9223013292642648 1107 1 0.24458975287352303 1107 2 0.32172302800502806 1107 3 0.37681820930739107 1107 4 0.327924153553397 1107 5 0.3631468064408523 1107 6 0.21838740644795251 1107 7 0.17220402359964737 1107 8 0.3002366091113018 1107 9 0.42512353354565136 1107 10-11 0.36256937591013383 1107 12-13 0.39868869795277107 1107 14-15 0.4643019918023583 1107 16-17 0.48732656628357063 1107 18-19 0.41491635332356225 1107 20-21 0.36972921446660933 1107 22-23 0.3419330421303286 1107 24-25 0.341812210346653 1107 26-27 0.35645948601442257 1107 28-29 0.43238812418208283 1107 30-31 0.515768728136706 1107 32-33 0.5543734446975819 1107 34-35 0.6398232010992899 1107 36-37 0.4991644667448938 1107 38-39 0.63222015401616 1107 40-41 0.5786602664901537 1107 42-43 0.5425408117062673 1107 44-45 0.1799259278589247 1107 46-47 0.4190183930179501 1107 48-49 0.5210985483302082 1107 50-51 0.49196253326226014 1107 52-53 0.6021926336013266 1107 54-55 0.6310641664539389 1107 56-57 0.4822327277091958 1107 58-59 0.480432342561798 1107 60-61 0.4707091032002708 1107 62-63 0.32983626923191167 1107 64-65 0.34444419412376703 1107 66-67 0.2752130459600295 1107 68-69 0.6227725870589467 1107 70-71 0.4443018701739305 1107 72-73 0.4375437317956461 1107 74-75 0.5396110080641421 1107 76-77 0.6235688384797591 1107 78-79 0.6694723410142238 1107 80-81 0.624009058285484 1107 82-83 0.6968320967358714 1107 84-85 0.7726092618590137 1107 86-87 0.7773104994453242 1107 88-89 0.7004074724714222 1107 90-91 0.8705945228652503 1107 92-93 0.8967081180452858 1107 94-95 0.9010341516361713 1107 96-97 0.9016028451979601 1107 98-99 0.9145172580099761 1107 100-101 0.9708947360191473 1108 1 0.2249226359919696 1108 2 0.2701603340144523 1108 3 0.20358839091078096 1108 4 0.18660260983050847 1108 5 0.25304175525555905 1108 6 0.27094192132573625 1108 7 0.4345923790863111 1108 8 0.31406496249110916 1108 9 0.3968020857599228 1108 10-11 0.3280204780587539 1108 12-13 0.36022377709151243 1108 14-15 0.4929680373856655 1108 16-17 0.3950329064127729 1108 18-19 0.5060899233896592 1108 20-21 0.3748988868506302 1108 22-23 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0.930070088771707 1108 90-91 0.9144978812300302 1108 92-93 0.9017525783329461 1108 94-95 1.0529230084415033 1108 96-97 0.9182626379360812 1108 98-99 1.1547504793539254 1108 100-101 1.2221278106356763 1109 1 0.09158466032456047 1109 2 0.1818291111402779 1109 3 0.1640683812114574 1109 4 0.23446462343615337 1109 5 0.18863996169204 1109 6 0.1771149411167272 1109 7 0.16257368715633191 1109 8 0.3146484092348629 1109 9 0.23920295671587866 1109 10-11 0.2828689340206978 1109 12-13 0.30942052935065334 1109 14-15 0.37946984260008776 1109 16-17 0.3124226842184896 1109 18-19 0.34445455529426283 1109 20-21 0.2707042901218557 1109 22-23 0.29672903475852763 1109 24-25 0.27866113459368336 1109 26-27 0.17136505221581189 1109 28-29 0.271851757309129 1109 30-31 0.2530207761511676 1109 32-33 0.3954498783077085 1109 34-35 0.28238992082061287 1109 36-37 0.29559983517807353 1109 38-39 0.3958552226207175 1109 40-41 0.4096368997533304 1109 42-43 0.3369821583870376 1109 44-45 0.3777315549684559 1109 46-47 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2316 58-59 -1.2172389290965242 2316 60-61 -1.3955835606930407 2316 62-63 -1.6303224606939786 2316 64-65 -1.6963980308822464 2316 66-67 -1.7057746778108793 2316 68-69 -1.773019368336488 2316 70-71 -1.5469702575493116 2316 72-73 -1.743556867567147 2316 74-75 -1.7294225377696542 2316 76-77 -1.5849712011843842 2316 78-79 -1.6503708066525746 2316 80-81 -1.639935184545923 2316 82-83 -1.5253630291508529 2316 84-85 -1.6648487490075574 2316 86-87 -1.7025959583000336 2316 88-89 -1.6819857321099043 2316 90-91 -1.8208365034738563 2316 92-93 -1.7885541728524963 2316 94-95 -1.9058442551677537 2316 96-97 -2.1128302318244145 2316 98-99 -2.1384425908438125 2316 100-101 -1.8058066856705715 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8210.0 3 2549.0 4 2646.0 5 2806.0 6 3417.0 7 4125.0 8 5038.0 9 5999.0 10 7520.0 11 8982.0 12 11181.0 13 14874.0 14 13725.0 15 14185.0 16 17302.0 17 22465.0 18 20151.0 19 15219.0 20 15270.0 21 16241.0 22 17664.0 23 19120.0 24 21850.0 25 24501.0 26 28108.0 27 32276.0 28 37733.0 29 44703.0 30 52274.0 31 63741.0 32 78322.0 33 99109.0 34 132120.0 35 186564.0 36 279246.0 37 409310.0 38 347956.0 39 49305.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69341445289857 19.6855708698589 15.99042498522693 21.630589692015594 2 15.192523164777446 21.007323973230875 39.73676059103512 24.063392270956548 3 19.124790038842814 25.41575121102596 30.57081314956733 24.888645600563898 4 12.889962069461674 17.93702490161166 38.05652039217542 31.116492636751236 5 13.800116715376195 37.4686356722501 33.9065667633045 14.824680849069209 6 30.029167357669763 36.63721822299302 18.15033229050426 15.183282128832959 7 27.111683495421264 33.77358411012016 21.078723716246255 18.03600867821232 8 26.041604689393722 33.90553094163135 20.14444472033278 19.908419648642152 9 26.12802799777929 17.642021031129726 21.07488796906002 35.155063002030964 10-11 24.744197791484275 26.947653806341076 27.116685535161338 21.191462867013303 12-13 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29.175688377197883 21.980968827603082 38-39 22.25458161670692 26.572318464110552 29.217279508960953 21.955820410221573 40-41 22.406954280520512 26.544906398704715 29.119744517436853 21.928394803337923 42-43 22.10312670620695 26.720415297682464 28.726779412861326 22.449678583249256 44-45 22.389567468516997 26.72511608287436 28.54094231274586 22.34437413586278 46-47 22.633142219424364 26.824827305858474 28.16540057230664 22.37662990241052 48-49 22.65770565845599 26.820932223748194 28.12637863851748 22.394983479278334 50-51 22.38699322114611 27.117486017378635 28.233837049641014 22.261683711834245 52-53 22.566350964575566 27.267155493181093 27.85021816030106 22.31627538194228 54-55 22.30634429474103 26.988677867485855 28.071958013513537 22.633019824259577 56-57 22.53469083717833 27.30187761061824 27.777248568606794 22.386182983596633 58-59 22.74863984892828 26.939986383791304 27.87651815435701 22.434855612923403 60-61 22.540446117330234 26.808013083237327 28.372143536672485 22.279397262759954 62-63 22.449348293292484 26.896310320038243 28.303840609438925 22.35050077723035 64-65 22.344433696987437 27.055545268218335 28.1304844284877 22.469536606306534 66-67 22.276687197120122 27.124388945652107 27.858195387730955 22.740728469496815 68-69 22.20398855303166 27.089377665232657 28.101436519217916 22.605197262517766 70-71 22.44194348989062 26.984610359261058 27.81504473054126 22.75840142030706 72-73 22.391017934622877 26.733899442150566 28.0501059718621 22.824976651364455 74-75 22.232351194314052 27.32859943769302 27.66394580618199 22.77510356181094 76-77 22.472886012330036 26.974741916618168 27.70350605703736 22.84886601401444 78-79 22.41372537997149 27.076639236009107 27.82495121212898 22.684684171890424 80-81 22.410329256700827 27.181172454656842 27.620120231373768 22.78837805726856 82-83 22.404569548777996 27.094609805917447 27.814994531775806 22.685826113528755 84-85 22.396111463719993 27.049835162534396 27.661335816285387 22.89271755746022 86-87 22.40557210503497 27.063750664860926 27.737680282877296 22.792996947226804 88-89 22.488255753551506 26.906134486678564 27.570230217343845 23.03537954242609 90-91 22.323686131266637 26.957820765429506 27.772219615689952 22.9462734876139 92-93 22.561365876064414 27.03630514316587 27.714555847441595 22.687773133328125 94-95 22.381115538861156 26.985110115115752 27.621873074756287 23.01190127126681 96-97 22.27769390486553 26.827561475602707 27.73061233709883 23.164132282432934 98-99 22.264424172030218 26.876497762650857 27.490945183534166 23.36813288178476 100-101 22.76635893951645 26.083987138838577 28.08202178172773 23.06763213991724 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1515.0 1 887.5 2 288.0 3 330.0 4 309.0 5 313.0 6 585.0 7 1240.0 8 2217.0 9 2908.5 10 2717.0 11 2352.0 12 2496.5 13 2757.5 14 2964.5 15 3195.0 16 3380.0 17 3574.5 18 3698.0 19 3775.5 20 4067.0 21 4482.0 22 5154.5 23 6221.0 24 7545.0 25 9367.0 26 12164.0 27 15598.0 28 18531.5 29 21635.0 30 25118.0 31 29178.0 32 33415.0 33 37145.5 34 41722.5 35 45920.5 36 50360.0 37 55072.0 38 57976.5 39 59266.0 40 60854.5 41 63729.5 42 66306.5 43 68994.5 44 72392.5 45 74776.5 46 76004.5 47 81388.5 48 96211.5 49 96842.5 50 83119.5 51 76551.0 52 72902.5 53 71382.5 54 69286.0 55 64136.5 56 58989.5 57 53937.0 58 48641.5 59 43236.5 60 36581.0 61 29372.0 62 22839.5 63 17584.0 64 13145.5 65 9639.5 66 7191.0 67 5625.0 68 4085.5 69 2843.0 70 2099.5 71 1431.5 72 949.0 73 641.5 74 446.5 75 319.5 76 210.5 77 118.5 78 73.5 79 48.0 80 36.0 81 30.0 82 24.5 83 17.5 84 16.0 85 13.5 86 9.0 87 8.0 88 7.0 89 6.0 90 5.5 91 5.5 92 4.0 93 4.5 94 4.0 95 3.0 96 1.5 97 1.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3248413249855324 2 0.08713313718623175 3 0.09757413105647876 4 0.10183480568514457 5 0.11316539096137676 6 0.05777662078872111 7 0.05950898300037644 8 0.060539036207306635 9 0.06353555462746721 10-11 0.06093701130998421 12-13 0.05890031519628131 14-15 0.057378645686043526 16-17 0.05897054609675384 18-19 0.06674276574904534 20-21 0.0681707940586531 22-23 0.08399615696512615 24-25 0.09298571222560788 26-27 0.08713313718623175 28-29 0.0847921071704813 30-31 0.07882248063031765 32-33 0.07100344037771114 34-35 0.32011244435371655 36-37 0.496977262043663 38-39 0.4697979035608003 40-41 0.4513271767365292 42-43 0.48227559354475025 44-45 0.49154607240712195 46-47 0.4859041900691634 48-49 0.5034853254874493 50-51 0.4997630877624061 52-53 0.4988500860562634 54-55 0.48981371019546666 56-57 0.46813577224961744 58-59 0.4527317947459795 60-61 0.4127470020769618 62-63 0.4830013128496328 64-65 0.5038598902899694 66-67 0.515073424065414 68-69 0.5258889827381811 70-71 0.5244375441284157 72-73 0.512943086751081 74-75 0.514745679863209 76-77 0.4884793230864889 78-79 0.4694935696587527 80-81 0.47572070950064893 82-83 0.48461662356050067 84-85 0.4862319342713684 86-87 0.48541257376585584 88-89 0.4923654329126346 90-91 0.5060838688049323 92-93 0.5119364438443085 94-95 0.5188190720906147 96-97 0.5105318258348581 98-99 0.5139029090575388 100-101 0.49154607240712195 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2135812.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.970041972336105 #Duplication Level Percentage of deduplicated Percentage of total 1 79.03418498775318 47.396833909649345 2 12.448818512762335 14.93112337432704 3 3.6953860633097024 6.648373719620064 4 1.6716626243751365 4.0099871098944995 5 0.8605752182132241 2.580436597829968 6 0.4926665929220143 1.7727141753540616 7 0.34349956104839097 1.4419778165485726 8 0.23236900236125466 1.114814305973945 9 0.18749548653602974 1.0119700977470356 >10 0.9350608857828917 10.213276937409166 >50 0.06407202817980966 2.6217953939760714 >100 0.03107270273931224 3.3073657215312546 >500 0.0018033920596535501 0.7498992114302074 >1k 0.0011761252562957419 1.4399870485852726 >5k 1.5681670083943225E-4 0.7594445801234567 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8768 0.41052302356199893 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7383 0.34567649212571144 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4496 0.21050541901628048 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3806 0.17819920479892423 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2941 0.13769938552644145 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2542 0.11901796600075289 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2378 0.1113393875490914 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.6820600315009E-5 2 0.0 0.0 0.0 0.0 4.6820600315009E-5 3 0.0 0.0 0.0 0.0 4.6820600315009E-5 4 0.0 0.0 0.0 0.0 4.6820600315009E-5 5 0.0 0.0 0.0 0.0 4.6820600315009E-5 6 4.6820600315009E-5 0.0 0.0 0.0 4.6820600315009E-5 7 4.6820600315009E-5 0.0 0.0 4.6820600315009E-5 4.6820600315009E-5 8 4.6820600315009E-5 0.0 0.0 4.6820600315009E-5 4.6820600315009E-5 9 4.6820600315009E-5 0.0 0.0 4.6820600315009E-5 4.6820600315009E-5 10-11 4.6820600315009E-5 0.0 0.0 4.6820600315009E-5 4.6820600315009E-5 12-13 4.6820600315009E-5 0.0 0.0 4.6820600315009E-5 9.3641200630018E-5 14-15 4.6820600315009E-5 0.0 0.0 4.6820600315009E-5 1.40461800945027E-4 16-17 4.6820600315009E-5 0.0 0.0 7.02309004725135E-5 1.40461800945027E-4 18-19 4.6820600315009E-5 0.0 0.0 9.3641200630018E-5 1.87282401260036E-4 20-21 4.6820600315009E-5 0.0 0.0 1.638721011025315E-4 1.87282401260036E-4 22-23 4.6820600315009E-5 0.0 0.0 2.575133017325495E-4 1.87282401260036E-4 24-25 4.6820600315009E-5 0.0 0.0 5.384369036226035E-4 1.87282401260036E-4 26-27 4.6820600315009E-5 0.0 0.0 7.023090047251349E-4 1.87282401260036E-4 28-29 4.6820600315009E-5 0.0 0.0 0.0015450798103952969 1.87282401260036E-4 30-31 4.6820600315009E-5 0.0 0.0 0.0043777261294533415 1.87282401260036E-4 32-33 4.6820600315009E-5 0.0 0.0 0.008755452258906683 1.87282401260036E-4 34-35 4.6820600315009E-5 0.0 0.0 0.015848773206630546 1.87282401260036E-4 36-37 4.6820600315009E-5 0.0 0.0 0.02856056619215549 1.87282401260036E-4 38-39 4.6820600315009E-5 0.0 0.0 0.04831885952508928 1.87282401260036E-4 40-41 7.02309004725135E-5 0.0 0.0 0.06985633566999343 1.87282401260036E-4 42-43 9.3641200630018E-5 0.0 0.0 0.09382848303127803 1.87282401260036E-4 44-45 9.3641200630018E-5 0.0 0.0 0.1270476989547769 1.87282401260036E-4 46-47 9.3641200630018E-5 0.0 0.0 0.16714954312458213 2.1069270141754049E-4 48-49 9.3641200630018E-5 0.0 0.0 0.2129166799325034 2.34103001575045E-4 50-51 9.3641200630018E-5 0.0 0.0 0.26151646305948273 2.34103001575045E-4 52-53 9.3641200630018E-5 0.0 0.0 0.31166132599685736 2.34103001575045E-4 54-55 9.3641200630018E-5 0.0 0.0 0.3727387991077866 2.34103001575045E-4 56-57 9.3641200630018E-5 0.0 0.0 0.4480497347144786 2.575133017325495E-4 58-59 9.3641200630018E-5 0.0 0.0 0.5339187156922052 2.80923601890054E-4 60-61 9.3641200630018E-5 0.0 0.0 0.6336465943631743 2.80923601890054E-4 62-63 9.3641200630018E-5 0.0 0.0 0.7320634962253232 2.80923601890054E-4 64-65 9.3641200630018E-5 0.0 0.0 0.8390485679451187 2.80923601890054E-4 66-67 9.3641200630018E-5 0.0 0.0 0.9683670660151737 2.80923601890054E-4 68-69 9.3641200630018E-5 0.0 0.0 1.1220088659488756 2.80923601890054E-4 70-71 1.40461800945027E-4 0.0 0.0 1.2888072545710951 2.80923601890054E-4 72-73 1.40461800945027E-4 0.0 0.0 1.4628394259419837 2.80923601890054E-4 74-75 1.40461800945027E-4 0.0 0.0 1.6558339404404507 2.80923601890054E-4 76-77 1.40461800945027E-4 4.6820600315009E-5 0.0 1.8623127878296404 2.80923601890054E-4 78-79 1.40461800945027E-4 4.6820600315009E-5 0.0 2.1005594125325637 3.043339020475585E-4 80-81 1.40461800945027E-4 4.6820600315009E-5 0.0 2.3688882729378804 3.27744202205063E-4 82-83 1.40461800945027E-4 4.6820600315009E-5 0.0 2.649226617323997 3.27744202205063E-4 84-85 1.40461800945027E-4 4.6820600315009E-5 0.0 2.9451796319151686 3.27744202205063E-4 86-87 1.40461800945027E-4 4.6820600315009E-5 0.0 3.255623622303836 3.511545023625675E-4 88-89 1.40461800945027E-4 4.6820600315009E-5 0.0 3.598139723908284 3.74564802520072E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 7235 0.0 23.292858 12-13 GTACTTT 7645 0.0 21.82746 14-15 GTACATG 9890 0.0 21.067375 1 GGTATCA 14965 0.0 20.995367 1 GTATCAA 19250 0.0 20.833786 1 TACATGG 9905 0.0 20.606636 2 ACATGGG 9975 0.0 19.939796 3 GTGTAGC 1265 0.0 19.134708 1 AGTACTT 7670 0.0 18.639013 12-13 TATCAAC 21495 0.0 18.638771 2 ATCAACG 21675 0.0 18.39659 3 CAACGCA 21785 0.0 18.3037 5 ACTTTTT 9260 0.0 18.27794 16-17 TCAACGC 21840 0.0 18.257605 4 GCGTAGC 130 0.0076005696 18.254456 1 AACGCAG 22110 0.0 18.091272 6 GACCGGT 135 0.009590853 17.53224 7 GTAGCCC 1285 0.0 17.321243 3 CATGGGG 5015 0.0 17.280825 4 ACGCAGA 23630 0.0 16.727226 7 >>END_MODULE