FastQCFastQC Report
Thu 26 May 2016
SRR938917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135812
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86010.40270398330939244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79270.37114689869707634No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49930.2337752573728399No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39520.18503501244491555No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26530.12421505263571887No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25810.12084396941303822No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24230.11344631456326681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGACG1751.5016121E-724.4045414
GAGTACT77950.023.13498512-13
GTACTTT80450.022.15086614-15
GGTATCA157400.021.1607821
GTATCAA198000.019.9502621
AGTACTT79700.019.7093112-13
TTTGCGT4001.8189894E-1218.981313
ACTTTTT97500.018.251916-17
TACATGG91150.018.2212262
GTACATG94600.018.183411
TATAGCG1600.001199360417.7949755
ACATGGG92950.017.6641563
TATCAAC225800.017.4849972
TCAACGC227250.017.4360754
ATCAACG226450.017.4348073
CAACGCA228050.017.4165275
AACGCAG230900.017.2221056
CATGGGG46350.017.0975114
CTTTGCG4507.2759576E-1216.8722742
AGAGTAC170700.016.3331910-11
Produced by FastQC (version 0.11.5)