##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938917_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2135812 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1821934702118 33.0 31.0 34.0 30.0 34.0 2 32.42929059299227 34.0 31.0 34.0 31.0 34.0 3 32.50797308002764 34.0 31.0 34.0 30.0 34.0 4 36.057772406934696 37.0 35.0 37.0 35.0 37.0 5 35.98322558352514 37.0 35.0 37.0 35.0 37.0 6 36.02501811957232 37.0 35.0 37.0 35.0 37.0 7 35.972338857539896 37.0 35.0 37.0 35.0 37.0 8 35.94290415073986 37.0 35.0 37.0 35.0 37.0 9 37.613648579556624 39.0 37.0 39.0 35.0 39.0 10-11 37.524477341638686 39.0 37.0 39.0 35.0 39.0 12-13 37.429554661178045 39.0 37.0 39.0 34.0 39.0 14-15 38.731434929666094 40.0 38.0 41.0 34.5 41.0 16-17 38.82430780424494 40.0 38.0 41.0 35.0 41.0 18-19 38.88008612181222 40.0 38.0 41.0 35.0 41.0 20-21 38.85990082460441 40.0 38.0 41.0 34.5 41.0 22-23 38.784979202289335 40.0 38.0 41.0 34.0 41.0 24-25 38.70582031564576 40.0 38.0 41.0 34.0 41.0 26-27 38.53879718814203 40.0 38.0 41.0 34.0 41.0 28-29 38.38051312568709 40.0 38.0 41.0 34.0 41.0 30-31 38.18591055767081 40.0 38.0 41.0 33.5 41.0 32-33 38.07882224652732 40.0 37.0 41.0 33.0 41.0 34-35 37.979141188456666 40.0 37.0 41.0 33.0 41.0 36-37 37.845953670079574 40.0 37.0 41.0 33.0 41.0 38-39 37.69578970433727 40.0 37.0 41.0 33.0 41.0 40-41 37.52211196491077 40.0 37.0 41.0 32.0 41.0 42-43 37.33147837918319 40.0 37.0 41.0 31.5 41.0 44-45 37.06528102660721 40.0 36.0 41.0 31.0 41.0 46-47 36.730630317649684 40.0 36.0 41.0 30.5 41.0 48-49 36.360714800740894 39.0 35.5 41.0 30.0 41.0 50-51 36.076384766074916 39.0 35.0 40.5 29.0 41.0 52-53 35.79804285208623 39.0 35.0 40.0 28.5 41.0 54-55 35.480742452987435 38.5 35.0 40.0 28.0 41.0 56-57 35.325660685491044 38.0 35.0 40.0 27.5 41.0 58-59 35.02810827919311 38.0 34.0 40.0 27.0 41.0 60-61 34.64204059158765 38.0 34.0 40.0 26.0 41.0 62-63 34.204251357329206 37.0 33.5 40.0 26.0 41.0 64-65 33.765419896507744 36.5 33.0 39.0 25.0 41.0 66-67 33.28341960809284 36.0 32.5 39.0 23.0 40.0 68-69 32.768738774761076 35.5 32.0 38.5 22.0 40.0 70-71 32.215699228209225 35.0 31.0 38.0 21.0 40.0 72-73 31.67781808511236 35.0 31.0 37.0 19.5 39.0 74-75 31.130507273112052 34.5 30.5 36.5 18.0 39.0 76-77 29.243311911348002 32.5 28.5 35.0 13.0 37.0 78-79 30.187378851696685 34.0 29.5 35.5 13.0 37.5 80-81 30.158307706858096 34.0 30.0 35.0 10.0 37.0 82-83 29.708697441535115 34.0 29.5 35.0 7.0 37.0 84-85 29.21633926581553 34.0 29.0 35.0 2.0 36.0 86-87 28.478735956160936 33.5 28.5 35.0 2.0 36.0 88-89 27.73541631941388 33.0 26.5 35.0 2.0 35.0 90-91 27.09705980676202 32.5 25.5 34.5 2.0 35.0 92-93 26.635893749075294 32.0 25.0 34.0 2.0 35.0 94-95 26.002222807999956 32.0 24.5 34.0 2.0 35.0 96-97 25.05610255022446 32.0 20.0 34.0 2.0 35.0 98-99 23.71997020336996 31.0 2.0 34.0 2.0 35.0 100-101 18.023905428005836 23.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12863362847331672 1101 2 0.15031662171296034 1101 3 0.2227480712792982 1101 4 0.1408099976386623 1101 5 0.035075439567677336 1101 6 0.15570476808404266 1101 7 0.13959914755397307 1101 8 0.12940469384260922 1101 9 0.24550668125949926 1101 10-11 0.2762757461769141 1101 12-13 0.23553168305750916 1101 14-15 0.26598689307597567 1101 16-17 0.3004206891521548 1101 18-19 0.2562711073917825 1101 20-21 0.31714796490389574 1101 22-23 0.36459824304703403 1101 24-25 0.2821800066998108 1101 26-27 0.19907635776205268 1101 28-29 0.3349577496652074 1101 30-31 0.41636275604827944 1101 32-33 0.4108205265209577 1101 34-35 0.4674750269597965 1101 36-37 0.5338478883336606 1101 38-39 0.4404534605376469 1101 40-41 0.43771183661395696 1101 42-43 0.4167230741497647 1101 44-45 0.3738818537591655 1101 46-47 0.4288996498334967 1101 48-49 0.48024604016504213 1101 50-51 0.47531860397940306 1101 52-53 0.27929052691082035 1101 54-55 0.2549107483569486 1101 56-57 0.2787135722482219 1101 58-59 0.2621410200290839 1101 60-61 0.2067211174863388 1101 62-63 0.23685163177015056 1101 64-65 0.2450665742185052 1101 66-67 0.17209511693290835 1101 68-69 0.32126126179992553 1101 70-71 0.44372418381243506 1101 72-73 0.5000410211659236 1101 74-75 0.46920184252040187 1101 76-77 0.29629881535730007 1101 78-79 0.6787584080821425 1101 80-81 0.785030768355508 1101 82-83 0.5637491923281956 1101 84-85 0.737722945842382 1101 86-87 0.8419207815414929 1101 88-89 0.9632826762006168 1101 90-91 0.7868829246572737 1101 92-93 0.7187954904263272 1101 94-95 0.6403597310227731 1101 96-97 0.8587091940875453 1101 98-99 1.0910248667972269 1101 100-101 1.0718749915366352 1102 1 0.1272683518588451 1102 2 0.12575686229306626 1102 3 0.14234695141314546 1102 4 0.10626964700309571 1102 5 0.050230209590175434 1102 6 0.04745170587987246 1102 7 0.15924146603917677 1102 8 0.1403521064912283 1102 9 0.06261450542724134 1102 10-11 0.18049892199694995 1102 12-13 0.16938413088719528 1102 14-15 0.2275400848466873 1102 16-17 0.12013269363121992 1102 18-19 0.1081060346705911 1102 20-21 0.12212436323333264 1102 22-23 0.13961278279388267 1102 24-25 0.14352933013687874 1102 26-27 0.0954901114269262 1102 28-29 0.1811691444853949 1102 30-31 0.2242983764694415 1102 32-33 0.15908118357529588 1102 34-35 0.2077806772638553 1102 36-37 0.18939773028642293 1102 38-39 0.20366791350758007 1102 40-41 0.07544085166119174 1102 42-43 0.0884155905027697 1102 44-45 0.020742528428371543 1102 46-47 0.07161147541941659 1102 48-49 0.031986954975231185 1102 50-51 -0.09254490029545082 1102 52-53 -0.06861723408292875 1102 54-55 -0.007258913709648596 1102 56-57 -0.05122166443838694 1102 58-59 -0.22792616844670022 1102 60-61 -0.14276274956985446 1102 62-63 -0.02055316002142149 1102 64-65 0.00631469952055852 1102 66-67 -0.08251687080455383 1102 68-69 0.035252402971075014 1102 70-71 0.16912107336700188 1102 72-73 0.25634139100319686 1102 74-75 0.042407440653530415 1102 76-77 -0.18667489782832192 1102 78-79 0.13227513059989704 1102 80-81 0.14734416870016886 1102 82-83 0.2164085500743269 1102 84-85 0.2122757067226182 1102 86-87 0.4936189316565489 1102 88-89 0.41609704972901795 1102 90-91 0.5423092879191564 1102 92-93 0.507990992698172 1102 94-95 0.6098029341932438 1102 96-97 0.7427229326824829 1102 98-99 0.9305321346746283 1102 100-101 1.1831663680633078 1103 1 0.0974641073394551 1103 2 0.07749093931221296 1103 3 0.11589990131993488 1103 4 0.09665054968241549 1103 5 0.1080654564363499 1103 6 0.11590956334880786 1103 7 0.08981931071783578 1103 8 0.07684092606597659 1103 9 0.1848372471703783 1103 10-11 0.14894167146251647 1103 12-13 0.17069985950019628 1103 14-15 0.21394501855247228 1103 16-17 0.14514825412054222 1103 18-19 0.201261832624553 1103 20-21 0.23509816630741653 1103 22-23 0.20950590058301088 1103 24-25 0.1489831685249854 1103 26-27 0.15713730108691948 1103 28-29 0.15276710367317037 1103 30-31 0.1734767876605261 1103 32-33 0.29933197140363177 1103 34-35 0.25203103874985544 1103 36-37 0.41723119735449643 1103 38-39 0.27326415023214423 1103 40-41 0.4369925919221842 1103 42-43 0.41788016889646684 1103 44-45 0.32200994159631335 1103 46-47 0.42588422490509004 1103 48-49 0.1789106537764411 1103 50-51 0.30210819883115647 1103 52-53 0.1986390720630382 1103 54-55 0.2985456767101198 1103 56-57 0.4094510212970377 1103 58-59 0.2857372735033721 1103 60-61 0.2753059951134418 1103 62-63 0.34927329150325903 1103 64-65 0.42306293935018147 1103 66-67 0.36188416850541927 1103 68-69 0.2617785626925908 1103 70-71 0.31856172530876137 1103 72-73 0.17699772691268123 1103 74-75 0.26640935718294045 1103 76-77 0.08897062366677133 1103 78-79 0.3505753469887374 1103 80-81 0.4426913058717936 1103 82-83 0.44328790022404974 1103 84-85 0.5852494494652483 1103 86-87 0.5281214887049757 1103 88-89 0.41792898940560264 1103 90-91 0.5059490246326597 1103 92-93 0.7065139277030887 1103 94-95 0.9423010538627103 1103 96-97 0.7469186087266699 1103 98-99 0.8214435520014227 1103 100-101 1.2443766573928876 1104 1 0.036222125974475716 1104 2 0.03673233495950967 1104 3 0.03634299774746097 1104 4 -0.01021269223497967 1104 5 0.03455139021626508 1104 6 0.09429922952971026 1104 7 0.054857912111963 1104 8 0.062121270939748285 1104 9 0.12566951601679222 1104 10-11 0.18262381167092911 1104 12-13 0.15619834922814846 1104 14-15 0.1460046408145459 1104 16-17 0.19474972121340528 1104 18-19 0.14112781721998147 1104 20-21 0.07802025286169822 1104 22-23 0.139938806922153 1104 24-25 0.24035675145800894 1104 26-27 0.1253941013092117 1104 28-29 0.07215196017234149 1104 30-31 0.04787620214274568 1104 32-33 0.15345103601448784 1104 34-35 0.11108972246430682 1104 36-37 0.04364051806191327 1104 38-39 0.12695029850868167 1104 40-41 0.2433320612979415 1104 42-43 0.0010565778673381487 1104 44-45 0.04538202625150234 1104 46-47 -0.06383260658213885 1104 48-49 -0.019637146183974608 1104 50-51 -0.07579902488701151 1104 52-53 -0.16881581468155815 1104 54-55 0.014696991776261825 1104 56-57 -0.054449128830597715 1104 58-59 0.04379001708097263 1104 60-61 0.07739501320671849 1104 62-63 0.07309228574803228 1104 64-65 -0.008115357710636317 1104 66-67 -0.029252803474086875 1104 68-69 -0.11615439862006127 1104 70-71 -0.16723824410598098 1104 72-73 -0.09053457251899033 1104 74-75 0.02677361161230607 1104 76-77 0.08066900004935462 1104 78-79 0.39262870227149094 1104 80-81 0.35993969214960586 1104 82-83 0.3227476256364028 1104 84-85 0.4290935915887424 1104 86-87 0.5466055793732707 1104 88-89 0.4465808042308588 1104 90-91 0.6492934326280064 1104 92-93 0.6509484602583946 1104 94-95 1.033144392373547 1104 96-97 0.866934636001524 1104 98-99 0.8795767817846709 1104 100-101 1.2205311509270054 1105 1 -0.06242290324517086 1105 2 -0.09662189675899668 1105 3 0.022592646199889543 1105 4 0.014400088194122418 1105 5 -0.01598172309861212 1105 6 -0.03872516696927164 1105 7 -0.004238166168924806 1105 8 -0.006092790433662287 1105 9 -0.04368559237533276 1105 10-11 0.044256012441699966 1105 12-13 0.03601467118773627 1105 14-15 0.00867674875991753 1105 16-17 -0.11117052025196017 1105 18-19 0.009045892983927217 1105 20-21 -0.01641161860312934 1105 22-23 -0.05085126029830889 1105 24-25 -0.12536494893276284 1105 26-27 -0.143773932052504 1105 28-29 -0.05401361999481935 1105 30-31 0.022465177177998896 1105 32-33 0.09534710977247585 1105 34-35 0.17418339879419165 1105 36-37 0.12537770314566643 1105 38-39 0.1547292324071492 1105 40-41 0.25125556454734266 1105 42-43 0.2655419615479744 1105 44-45 0.12610961724428904 1105 46-47 0.04979273958245756 1105 48-49 0.19183378176446553 1105 50-51 0.19955504275591096 1105 52-53 0.2526521276687319 1105 54-55 0.2190129493774009 1105 56-57 0.2347117063766504 1105 58-59 0.3323768283394415 1105 60-61 0.29067450152186325 1105 62-63 0.2292304085165 1105 64-65 0.17284930084255024 1105 66-67 0.1490177196262863 1105 68-69 0.16499740713754818 1105 70-71 0.3588382252185056 1105 72-73 0.25670733382753497 1105 74-75 0.1378882645960644 1105 76-77 0.03012710556342313 1105 78-79 0.3102847743550434 1105 80-81 0.34991857162887285 1105 82-83 0.35406501059885187 1105 84-85 0.33795129114806954 1105 86-87 0.3963260614667803 1105 88-89 0.5410130365657295 1105 90-91 0.4565610425448128 1105 92-93 0.5581163256952522 1105 94-95 0.44351870843459906 1105 96-97 0.5724705749288397 1105 98-99 0.7839802624002914 1105 100-101 0.7536811542590378 1106 1 -0.10054931304081549 1106 2 -0.07888190529045147 1106 3 -0.10055156382843222 1106 4 -0.061481980438067296 1106 5 -0.0769914217018055 1106 6 0.008489990930165447 1106 7 -0.014251930937909663 1106 8 -0.07517667272185946 1106 9 -0.11312022274779565 1106 10-11 -0.056957720136608714 1106 12-13 -0.07053843342125532 1106 14-15 -0.2275522022846772 1106 16-17 -0.21252473813722617 1106 18-19 -0.1801879317843813 1106 20-21 -0.15011327619019 1106 22-23 -0.21172449742866917 1106 24-25 -0.06614225134506313 1106 26-27 -0.17423051860377825 1106 28-29 -0.2162585658963465 1106 30-31 -0.12106591636055697 1106 32-33 -0.05547561312118887 1106 34-35 -0.2053939451124407 1106 36-37 -0.12007152653432485 1106 38-39 -0.1156768399074295 1106 40-41 -0.11176765599924465 1106 42-43 -0.05169688812855355 1106 44-45 0.0026386160631872713 1106 46-47 0.17857810920068573 1106 48-49 0.2496546499729675 1106 50-51 0.13126964732001056 1106 52-53 0.23972352537193586 1106 54-55 0.24508157970613098 1106 56-57 0.23853202287795483 1106 58-59 0.14944402543893176 1106 60-61 0.2842124099933585 1106 62-63 0.32384168055513385 1106 64-65 0.45317110058028476 1106 66-67 0.3204189814820566 1106 68-69 0.2902140080350719 1106 70-71 0.34649854477140707 1106 72-73 0.3303797825655117 1106 74-75 0.4097647808023446 1106 76-77 0.15716955026826795 1106 78-79 0.2605064573783231 1106 80-81 0.17854231336663773 1106 82-83 0.2478871924845265 1106 84-85 0.3487473776553891 1106 86-87 0.6073285962496868 1106 88-89 0.6102176338776211 1106 90-91 0.7907020888626306 1106 92-93 0.7435470457382714 1106 94-95 0.7019360809779087 1106 96-97 0.6365650192001304 1106 98-99 0.4144744822672415 1106 100-101 0.4761497555603391 1107 1 0.037920589757675316 1107 2 0.06205706641604536 1107 3 0.044214303968701074 1107 4 0.040140156966515406 1107 5 0.027274736303908753 1107 6 0.12317335132514273 1107 7 0.07237165750958496 1107 8 0.12479035737901256 1107 9 -0.011173896641508918 1107 10-11 0.008012496748385445 1107 12-13 0.11700128658257114 1107 14-15 0.14147671020457864 1107 16-17 0.06829408170570161 1107 18-19 0.06835712217972656 1107 20-21 0.15296045515747636 1107 22-23 0.17391793804583955 1107 24-25 0.09323920028561616 1107 26-27 0.15710089592428744 1107 28-29 0.21056892163203145 1107 30-31 0.277583098399667 1107 32-33 0.2736730379599308 1107 34-35 0.24919047803371086 1107 36-37 0.1913936870077677 1107 38-39 0.25577338564708896 1107 40-41 0.28628096679572224 1107 42-43 0.33655007753866784 1107 44-45 0.26484138186118855 1107 46-47 0.03779273681531947 1107 48-49 0.16318649760979298 1107 50-51 0.23214514049092116 1107 52-53 0.14979100805125967 1107 54-55 0.18919989664328796 1107 56-57 0.2823337736806195 1107 58-59 0.33886390215538853 1107 60-61 0.3955005136455796 1107 62-63 0.37226794186041445 1107 64-65 0.3660869437647918 1107 66-67 0.2620976177221266 1107 68-69 0.2430509806085226 1107 70-71 0.48172679014827935 1107 72-73 0.47606517993086683 1107 74-75 0.14474809380883258 1107 76-77 0.2958630789842225 1107 78-79 0.43680289075157575 1107 80-81 0.38123555922106434 1107 82-83 0.4836391908828688 1107 84-85 0.7150041553115294 1107 86-87 0.6624948600529592 1107 88-89 0.7769011375401256 1107 90-91 0.9057668072133644 1107 92-93 1.0247075035331257 1107 94-95 0.9179814471917531 1107 96-97 1.0577113982051962 1107 98-99 0.9538280518328079 1107 100-101 1.1026168701523211 1108 1 0.04129908252853198 1108 2 0.009311617787567172 1108 3 0.08851163954934549 1108 4 0.10066686135591851 1108 5 -0.028789009233129548 1108 6 0.01967667714653487 1108 7 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-0.4464192592654328 2316 50-51 -0.6736462794972198 2316 52-53 -0.5293482037118551 2316 54-55 -0.45352795216356157 2316 56-57 -0.4264556337628562 2316 58-59 -0.5191739548072576 2316 60-61 -0.3295212693633829 2316 62-63 -0.31994180871313915 2316 64-65 -0.3553923499083069 2316 66-67 -0.31325142382547 2316 68-69 -0.28010245098549547 2316 70-71 -0.2824466510097281 2316 72-73 -0.1810675086645226 2316 74-75 -0.2752827764553807 2316 76-77 -0.18874689514020204 2316 78-79 -0.17050648224981302 2316 80-81 -0.25200677932063087 2316 82-83 -0.2586811983068884 2316 84-85 -0.3865356540541356 2316 86-87 -0.5066579055823972 2316 88-89 -0.4861151271065651 2316 90-91 -0.7157664305778795 2316 92-93 -0.6464594569963538 2316 94-95 -0.5053726154494349 2316 96-97 -0.48317721654212065 2316 98-99 -0.585863230856372 2316 100-101 0.18138371714611878 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 16.0 5 41.0 6 86.0 7 270.0 8 619.0 9 1167.0 10 1986.0 11 3039.0 12 4417.0 13 5966.0 14 7684.0 15 10698.0 16 14200.0 17 20169.0 18 20784.0 19 14971.0 20 14087.0 21 14974.0 22 16215.0 23 17786.0 24 20426.0 25 23890.0 26 28198.0 27 34205.0 28 40615.0 29 49346.0 30 58596.0 31 71819.0 32 88006.0 33 111118.0 34 149288.0 35 212637.0 36 325557.0 37 461627.0 38 278493.0 39 12808.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.56503042741278 20.462329330559314 16.550669891950484 21.42197035007742 2 15.603292799178952 21.611546334602483 38.96813015377758 23.81703071244098 3 19.238444207636253 25.474573604792933 30.81436942951908 24.47261275805174 4 13.355201675053795 18.558281346860117 37.49688643007905 30.589630548007037 5 13.731545660385839 37.688382685367436 33.921150363421496 14.658921290825223 6 30.071888349723665 36.97675638117961 17.805218811393512 15.146136457703205 7 27.054581583023225 33.901204787687305 20.90862866207325 18.135584967216214 8 26.081040840673243 34.37250095045818 19.818457804338586 19.728000404529986 9 25.91941612838583 17.646356514524687 20.914715340114203 35.51951201697528 10-11 24.753021333338328 27.161332551741445 26.944974557685793 21.140671557234437 12-13 26.161150887812223 24.588095768728707 27.40030489574925 21.850448447709816 14-15 23.10915398985855 25.721372419573086 26.596481863853654 24.572991726714704 16-17 21.67667325761006 28.933812288746065 27.136067318456814 22.25344713518707 18-19 21.576774267857505 27.6411664275295 29.419419904499645 21.362639400113352 20-21 22.96894576863507 26.352436450399193 29.23581288989855 21.44280489106719 22-23 22.390828406245493 26.357961281236364 29.257163083642197 21.99404722887595 24-25 22.411749544259663 26.571950086395773 29.28352023739934 21.732780131945226 26-27 22.22988779674611 26.47169471650471 29.484671283022866 21.81374620372631 28-29 21.820295981106952 26.972622122171803 29.453715963764598 21.753365932956644 30-31 22.15831730508116 26.62008641209994 29.197958434543864 22.023637848275037 32-33 22.037239232666543 26.697504274720806 29.1578331800739 22.107423312538742 34-35 22.55134212772772 26.62886022981681 29.008928475266295 21.810869167189175 36-37 22.499901068715445 26.278537577848383 29.338101704170406 21.883459649265767 38-39 22.26499239431825 26.558099471594176 29.322133224572593 21.854774909514983 40-41 22.343419236994936 26.613848377594657 29.197068671579686 21.845663713830728 42-43 22.110268531999626 26.757774776426235 28.765449527310544 22.366507164263595 44-45 22.38874804725131 26.73300959395703 28.647376369616318 22.230865989175342 46-47 22.62121421397753 26.946204791212597 28.15400426541061 22.278576729399262 48-49 22.63857908330009 26.948450847197332 28.15280641723649 22.26016365226609 50-51 22.394164240757846 27.200027156132546 28.14182992535575 22.263978677753858 52-53 22.622871301406676 27.323893676035155 27.745162027369453 22.308072995188716 54-55 22.37528396694091 27.07064104893127 27.990899948122777 22.563175036005042 56-57 22.523635039039018 27.406344753189888 27.720323698902337 22.349696508868757 58-59 22.73368188450803 27.009680477068592 27.86065225875067 22.395985379672705 60-61 22.53479350279371 26.960156701569616 28.26183601818446 22.243213777452215 62-63 22.53383235278212 26.95578237264598 28.1889803227239 22.321404951847995 64-65 22.320400600244874 27.139402423921773 28.052809375431632 22.487387600401725 66-67 22.335145881359768 27.142592599962683 27.79659882116877 22.725662697508778 68-69 22.231222430683697 27.122635435839253 28.055561668413905 22.59058046506314 70-71 22.41626248764521 27.000165276796494 27.802694152244744 22.780878083313553 72-73 22.401690785518575 26.815445366914314 27.942955653400205 22.839908194166902 74-75 22.188750695285915 27.443145745037484 27.536248508763876 22.831855050912722 76-77 22.41101275320236 27.020217760004723 27.676201848612138 22.89256763818078 78-79 22.315299271378542 27.133523165564437 27.79916895528985 22.75200860776717 80-81 22.330786442782518 27.21685526828969 27.60698883372889 22.845369455198902 82-83 22.33644627897961 27.14042715370079 27.775431545473104 22.747695021846493 84-85 22.288814215128788 27.06204066000284 27.672511914689004 22.976633210179376 86-87 22.275897766833342 27.046171200461522 27.749303651194566 22.928627381510573 88-89 22.27316164263994 26.964604666518056 27.536350477007428 23.225883213834578 90-91 22.107696060896497 26.96211313929171 27.80945683795295 23.12073396185884 92-93 22.255282768333544 27.084827690826717 27.683756810056316 22.976132730783423 94-95 22.107359997715136 27.003240712886605 27.60446868625765 23.284930603140612 96-97 21.88514763592199 26.86260000004682 27.780237160442294 23.472015203588896 98-99 21.898556614533486 26.867322592063346 27.493290607974856 23.740830185428305 100-101 22.198819648797528 26.004433925381765 28.431738852563786 23.36500757325692 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 364.0 1 257.5 2 180.5 3 255.0 4 254.0 5 229.0 6 450.0 7 1005.5 8 1865.5 9 2517.5 10 2317.5 11 1930.5 12 1974.0 13 2129.5 14 2327.0 15 2526.5 16 2690.0 17 2842.0 18 3008.0 19 3295.5 20 3732.5 21 4327.0 22 5072.5 23 6262.5 24 7626.5 25 9336.5 26 12223.0 27 15689.0 28 18794.5 29 22086.5 30 25476.5 31 29365.0 32 33409.0 33 37258.0 34 42137.5 35 46307.5 36 50722.5 37 55474.0 38 58345.0 39 59580.0 40 61133.0 41 63933.5 42 66594.5 43 69382.5 44 72755.5 45 75296.0 46 76911.5 47 83102.5 48 98180.0 49 98195.0 50 83801.5 51 77223.5 52 73211.5 53 71188.0 54 69334.0 55 64238.5 56 58933.5 57 53785.0 58 48535.5 59 43311.5 60 36662.5 61 29175.0 62 22531.0 63 17160.0 64 12687.0 65 9242.5 66 6700.0 67 5186.0 68 3869.0 69 2698.0 70 1884.5 71 1204.5 72 807.5 73 567.0 74 398.0 75 247.0 76 137.5 77 60.5 78 29.5 79 19.0 80 9.0 81 7.0 82 6.0 83 4.5 84 1.5 85 2.5 86 3.5 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3958213550630861 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001030053206930198 16-17 0.002481491816695477 18-19 3.9797510267757647E-4 20-21 0.0 22-23 0.0 24-25 7.02309004725135E-5 26-27 2.34103001575045E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013343871089777564 36-37 0.02301232505482692 38-39 0.011119892574814636 40-41 0.005852575039376124 42-43 0.005033214533863468 44-45 0.0018962343127578642 46-47 0.001264156208505243 48-49 0.002996518420160576 50-51 6.788987045676305E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.661811058276666E-4 60-61 0.0015216695102377924 62-63 0.0011002841074027115 64-65 7.959502053551529E-4 66-67 0.0024580815165379725 68-69 0.0027624154185855307 70-71 4.6820600315009E-5 72-73 0.0 74-75 0.0 76-77 0.09340709762844296 78-79 0.0031135699209480982 80-81 2.34103001575045E-4 82-83 0.0 84-85 1.40461800945027E-4 86-87 0.09380507273112053 88-89 0.44151826097053487 90-91 0.39324622204576054 92-93 0.0 94-95 7.725399051976485E-4 96-97 2.34103001575045E-5 98-99 0.0 100-101 3.27744202205063E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2135812.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.09531472808545 #Duplication Level Percentage of deduplicated Percentage of total 1 77.71211899006202 45.14710010914078 2 13.075963587159881 15.193044399380767 3 4.057815261735183 7.072201642168014 4 1.7115393664753473 3.977296726595731 5 0.9008816686265403 2.616850203581082 6 0.5666477394239204 1.9751747257074483 7 0.3638973673181133 1.4798512458147255 8 0.27284669957741375 1.2680891907575382 9 0.19881243909081894 1.03950640987555 >10 1.0297998343072545 10.767414567255994 >50 0.07252030306236623 2.8764170095752726 >100 0.03401526438879444 3.582316268198793 >500 0.0016915654927716548 0.7029725856853086 >1k 0.0012888118040164638 1.528317246431637 >5k 1.6110147550205798E-4 0.773447669831351 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8601 0.40270398330939244 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7927 0.37114689869707634 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4993 0.2337752573728399 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3952 0.18503501244491555 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2653 0.12421505263571887 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2581 0.12084396941303822 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2423 0.11344631456326681 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.6820600315009E-5 2 0.0 0.0 0.0 0.0 4.6820600315009E-5 3 0.0 0.0 0.0 0.0 4.6820600315009E-5 4 0.0 0.0 0.0 0.0 4.6820600315009E-5 5 0.0 0.0 0.0 0.0 4.6820600315009E-5 6 0.0 0.0 0.0 0.0 4.6820600315009E-5 7 0.0 0.0 0.0 4.6820600315009E-5 4.6820600315009E-5 8 0.0 0.0 0.0 4.6820600315009E-5 4.6820600315009E-5 9 0.0 0.0 0.0 4.6820600315009E-5 4.6820600315009E-5 10-11 0.0 0.0 0.0 4.6820600315009E-5 4.6820600315009E-5 12-13 0.0 0.0 0.0 4.6820600315009E-5 2.1069270141754049E-4 14-15 0.0 0.0 0.0 9.3641200630018E-5 2.34103001575045E-4 16-17 0.0 0.0 0.0 9.3641200630018E-5 2.34103001575045E-4 18-19 0.0 0.0 0.0 9.3641200630018E-5 2.80923601890054E-4 20-21 0.0 0.0 0.0 1.638721011025315E-4 2.80923601890054E-4 22-23 0.0 0.0 0.0 2.575133017325495E-4 2.80923601890054E-4 24-25 0.0 0.0 0.0 4.916163033075945E-4 3.043339020475585E-4 26-27 0.0 0.0 0.0 6.55488404410126E-4 3.27744202205063E-4 28-29 0.0 0.0 0.0 0.0014982592100802879 3.27744202205063E-4 30-31 0.0 0.0 0.0 0.0043309055291383325 3.27744202205063E-4 32-33 0.0 0.0 0.0 0.008802272859221692 3.27744202205063E-4 34-35 0.0 0.0 0.0 0.015989235007575573 3.74564802520072E-4 36-37 0.0 0.0 0.0 0.028864900094203047 3.74564802520072E-4 38-39 0.0 0.0 0.0 0.04867001402745186 3.74564802520072E-4 40-41 0.0 0.0 0.0 0.07046500347408854 3.74564802520072E-4 42-43 2.34103001575045E-5 0.0 0.0 0.0947648950375782 3.74564802520072E-4 44-45 4.6820600315009E-5 0.0 0.0 0.12810116246186462 3.74564802520072E-4 46-47 9.3641200630018E-5 0.0 0.0 0.16864780233466242 3.9797510267757647E-4 48-49 9.3641200630018E-5 0.0 0.0 0.21457881124368622 4.6820600315009E-4 50-51 9.3641200630018E-5 0.0 0.0 0.2635765694733432 4.6820600315009E-4 52-53 9.3641200630018E-5 0.0 0.0 0.31428327961449787 4.6820600315009E-4 54-55 9.3641200630018E-5 0.0 0.0 0.3753139321251121 4.6820600315009E-4 56-57 1.40461800945027E-4 0.0 0.0 0.45097602223416666 5.15026603465099E-4 58-59 1.40461800945027E-4 0.0 0.0 0.5377814152181934 5.61847203780108E-4 60-61 1.40461800945027E-4 0.0 0.0 0.6378370380913676 5.61847203780108E-4 62-63 1.40461800945027E-4 0.0 0.0 0.7370498901588717 5.61847203780108E-4 64-65 1.40461800945027E-4 0.0 0.0 0.8449245532846523 6.08667804095117E-4 66-67 1.87282401260036E-4 0.0 0.0 0.975015591259905 6.08667804095117E-4 68-69 1.87282401260036E-4 0.0 0.0 1.1293362898981747 6.08667804095117E-4 70-71 1.87282401260036E-4 0.0 0.0 1.2968838081254344 6.788987045676305E-4 72-73 1.87282401260036E-4 0.0 0.0 1.4720162636037255 7.02309004725135E-4 74-75 1.87282401260036E-4 0.0 0.0 1.6662515240105402 7.02309004725135E-4 76-77 1.87282401260036E-4 0.0 0.0 1.87394770700792 7.02309004725135E-4 78-79 1.87282401260036E-4 0.0 0.0 2.113622360020451 7.02309004725135E-4 80-81 1.87282401260036E-4 0.0 0.0 2.3823960161287605 7.02309004725135E-4 82-83 1.87282401260036E-4 0.0 0.0 2.6631323356175542 7.02309004725135E-4 84-85 1.87282401260036E-4 0.0 0.0 2.9603495064172316 7.02309004725135E-4 86-87 1.87282401260036E-4 0.0 0.0 3.2729706547205466 7.725399051976484E-4 88-89 2.1069270141754049E-4 0.0 0.0 3.615182422422947 8.427708056701619E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGACG 175 1.5016121E-7 24.404541 4 GAGTACT 7795 0.0 23.134985 12-13 GTACTTT 8045 0.0 22.150866 14-15 GGTATCA 15740 0.0 21.160782 1 GTATCAA 19800 0.0 19.950262 1 AGTACTT 7970 0.0 19.70931 12-13 TTTGCGT 400 1.8189894E-12 18.98131 3 ACTTTTT 9750 0.0 18.2519 16-17 TACATGG 9115 0.0 18.221226 2 GTACATG 9460 0.0 18.18341 1 TATAGCG 160 0.0011993604 17.794975 5 ACATGGG 9295 0.0 17.664156 3 TATCAAC 22580 0.0 17.484997 2 TCAACGC 22725 0.0 17.436075 4 ATCAACG 22645 0.0 17.434807 3 CAACGCA 22805 0.0 17.416527 5 AACGCAG 23090 0.0 17.222105 6 CATGGGG 4635 0.0 17.097511 4 CTTTGCG 450 7.2759576E-12 16.872274 2 AGAGTAC 17070 0.0 16.33319 10-11 >>END_MODULE