FastQCFastQC Report
Thu 26 May 2016
SRR938916_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938916_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139783
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88650.41429434666973236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72200.33741739232436185No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45380.21207757982935654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37190.17380267064464014No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29870.13959359430372145No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25840.12075990883187687No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24010.11220763974664721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT4100.027.7074433
GAGTACT73900.023.50609812-13
GTACTTT80300.021.57419414-15
GTATCAA199550.021.1470411
AGTACTT78050.020.52790612-13
GGTATCA156100.020.3508821
TTGCGTT5850.020.2280144
GTACATG98600.019.6678891
TACATGG98750.019.2689482
TATCAAC224350.018.608512
CAACGCA223950.018.5994745
ATCAACG223750.018.5949443
TCAACGC224150.018.5828784
ACATGGG99300.018.3995463
AACGCAG227200.018.332136
CTTTGCG6300.018.0318282
ACTTTTT97600.017.9693716-17
ACGCAGA243250.016.6944857
CGCAGAG242250.016.529728
AGAGTAC167800.016.44544410-11
Produced by FastQC (version 0.11.5)