##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938916_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2139783 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63689495617079 33.0 31.0 34.0 30.0 34.0 2 31.71334663374744 33.0 31.0 34.0 30.0 34.0 3 31.745709728509855 34.0 31.0 34.0 28.0 34.0 4 35.27851842920521 37.0 35.0 37.0 33.0 37.0 5 35.31415568774965 37.0 35.0 37.0 33.0 37.0 6 35.39314921185933 37.0 35.0 37.0 33.0 37.0 7 35.304479940255625 37.0 35.0 37.0 33.0 37.0 8 35.28425031884074 37.0 35.0 37.0 33.0 37.0 9 36.85816085089002 39.0 37.0 39.0 33.0 39.0 10-11 36.74515406468787 39.0 37.0 39.0 32.0 39.0 12-13 36.606808260463794 39.0 37.0 39.0 32.0 39.0 14-15 37.762976666325514 40.0 38.0 41.0 32.0 41.0 16-17 37.67594447661281 40.0 37.5 41.0 32.0 41.0 18-19 37.62948205495604 40.0 37.5 41.0 32.0 41.0 20-21 37.514615734399236 40.0 37.0 41.0 32.0 41.0 22-23 37.604187433959424 40.0 37.5 41.0 32.0 41.0 24-25 37.52902981283616 40.0 37.0 41.0 32.0 41.0 26-27 37.435677823405456 40.0 37.0 41.0 31.5 41.0 28-29 37.29686865443833 40.0 37.0 41.0 31.0 41.0 30-31 37.10687251931621 40.0 37.0 41.0 30.5 41.0 32-33 36.903733462692244 40.0 36.0 41.0 30.0 41.0 34-35 36.58057732957033 39.5 36.0 41.0 30.0 41.0 36-37 36.31101681806052 39.0 35.0 41.0 30.0 41.0 38-39 36.09291502923428 39.0 35.0 41.0 29.0 41.0 40-41 35.93809582560475 39.0 35.0 41.0 28.0 41.0 42-43 35.719988896070305 39.0 35.0 41.0 27.0 41.0 44-45 35.62022761186532 39.0 35.0 41.0 26.5 41.0 46-47 35.36749380661497 39.0 35.0 41.0 25.0 41.0 48-49 35.14990865896215 39.0 35.0 41.0 24.0 41.0 50-51 33.99360729569307 38.0 33.5 40.0 23.0 40.5 52-53 34.14083157030409 38.0 33.5 39.5 23.0 40.5 54-55 34.61959390274622 39.0 34.0 40.0 23.0 41.0 56-57 34.50881911857417 38.5 34.0 40.0 23.0 41.0 58-59 34.295965759144735 38.0 34.0 40.0 22.0 41.0 60-61 34.013859816626265 38.0 34.0 40.0 22.0 41.0 62-63 33.652947518510054 37.5 33.5 40.0 20.5 41.0 64-65 33.23592275478401 37.0 33.0 40.0 19.0 41.0 66-67 32.83653786388619 36.0 32.5 39.0 18.0 41.0 68-69 32.44159781622716 36.0 32.0 39.0 14.5 41.0 70-71 32.01810627526249 35.0 31.5 39.0 12.5 40.0 72-73 31.572611568556248 35.0 31.0 38.0 8.0 40.0 74-75 31.098471433785576 35.0 31.0 37.0 7.0 39.0 76-77 30.680757114155966 35.0 30.5 36.5 4.5 39.0 78-79 30.24891052036585 35.0 30.0 36.0 2.0 39.0 80-81 29.80646098225848 34.0 30.0 36.0 2.0 37.5 82-83 29.40701183250825 34.0 29.0 35.0 2.0 37.0 84-85 29.042158947893313 34.0 29.0 35.0 2.0 36.5 86-87 28.63797824358825 34.0 29.0 35.0 2.0 36.0 88-89 28.21125413184421 34.0 28.5 35.0 2.0 36.0 90-91 27.76460907484544 34.0 27.0 35.0 2.0 35.5 92-93 27.336003931239755 34.0 26.0 35.0 2.0 35.0 94-95 26.836893974762862 33.0 25.5 35.0 2.0 35.0 96-97 26.223280117656792 33.0 24.5 35.0 2.0 35.0 98-99 25.47595293541448 33.0 21.0 35.0 2.0 35.0 100-101 22.76663871990758 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33115636891167455 1101 2 0.3371113365449432 1101 3 0.31354004700655125 1101 4 0.2874351903187886 1101 5 0.1670511362868723 1101 6 0.2182885851221954 1101 7 0.30904336157463064 1101 8 0.27930927039921016 1101 9 0.3136260616319291 1101 10-11 0.39671700447706826 1101 12-13 0.46448696276546286 1101 14-15 0.21092692232367227 1101 16-17 0.5605163671945519 1101 18-19 0.6055353004002271 1101 20-21 0.5797590312791314 1101 22-23 0.5044468272998301 1101 24-25 0.4880528968502631 1101 26-27 0.5763146839651867 1101 28-29 0.5064757702943083 1101 30-31 0.5265194852331021 1101 32-33 0.5004971268401661 1101 34-35 0.40794185669750505 1101 36-37 0.3839939837707149 1101 38-39 0.47298585272553595 1101 40-41 0.4851598325986757 1101 42-43 0.3432874231834191 1101 44-45 0.4313035314204612 1101 46-47 0.5417615863474055 1101 48-49 0.3192405607685771 1101 50-51 0.26236008583453696 1101 52-53 0.20869028647605603 1101 54-55 0.33431963240374785 1101 56-57 0.38301200257120627 1101 58-59 0.24008659324184123 1101 60-61 0.20996987274383372 1101 62-63 0.30102107346346685 1101 64-65 0.19982801106081638 1101 66-67 0.30131498194617734 1101 68-69 0.16182707626078496 1101 70-71 0.14827605439540292 1101 72-73 0.23032493189120373 1101 74-75 0.19474528754887288 1101 76-77 0.18939728072634665 1101 78-79 0.1495252740849473 1101 80-81 0.023744607727000044 1101 82-83 0.026544750918436222 1101 84-85 0.020955314866714758 1101 86-87 -0.15255944653713982 1101 88-89 -0.19539618469284292 1101 90-91 -0.11961539532435594 1101 92-93 -0.038451106749629815 1101 94-95 0.051740238341896116 1101 96-97 -0.0642085885203656 1101 98-99 -8.844335793121161E-4 1101 100-101 0.5774343225883491 1102 1 0.2915726830883507 1102 2 0.1487815751101671 1102 3 0.16383519862424478 1102 4 0.029172824969968758 1102 5 0.12224119888909968 1102 6 0.20029279620260354 1102 7 0.2124065860590605 1102 8 0.23637786965098684 1102 9 0.3335563156127179 1102 10-11 0.17564726785759888 1102 12-13 0.23919956592753522 1102 14-15 0.36216815707260963 1102 16-17 0.4633111428229242 1102 18-19 0.4417115829938041 1102 20-21 0.3845615898864665 1102 22-23 0.35325564612938365 1102 24-25 0.45459548704069874 1102 26-27 0.40182931934953814 1102 28-29 0.35601588835495335 1102 30-31 0.2947808166112935 1102 32-33 0.4080455824640268 1102 34-35 0.30987350183878704 1102 36-37 0.29448220590520435 1102 38-39 0.2934493065634385 1102 40-41 0.2244312122914991 1102 42-43 0.1895115504182101 1102 44-45 0.5115835964666147 1102 46-47 0.58419433125745 1102 48-49 0.6451135609753678 1102 50-51 0.54371712529462 1102 52-53 0.4545950577805513 1102 54-55 0.5205198843165917 1102 56-57 0.5699092446895264 1102 58-59 0.5744160632848931 1102 60-61 0.5189148937052366 1102 62-63 0.49568273007241714 1102 64-65 0.44230563969617265 1102 66-67 0.3893829608155812 1102 68-69 0.2422786864350499 1102 70-71 0.4013539969818325 1102 72-73 0.48652170590282395 1102 74-75 0.34714950426911884 1102 76-77 0.25924481705631663 1102 78-79 0.20189706172618216 1102 80-81 -0.006392581376829298 1102 82-83 -0.11763516937182317 1102 84-85 -0.05641412284125735 1102 86-87 0.09188648619836925 1102 88-89 0.20840812226484218 1102 90-91 0.2885097339219733 1102 92-93 0.18379947133395547 1102 94-95 0.064879890175785 1102 96-97 -0.09230321563295618 1102 98-99 -0.05948095943797682 1102 100-101 0.32327047584159274 1103 1 0.10928729870294518 1103 2 0.3305116585505772 1103 3 0.3509013258289322 1103 4 0.3560482660476154 1103 5 0.2857892767043353 1103 6 0.18610239686390173 1103 7 0.2734329883931039 1103 8 0.22965427643681124 1103 9 0.34674951629238393 1103 10-11 0.45680339973898754 1103 12-13 0.4587950819075459 1103 14-15 0.4671844693307463 1103 16-17 0.49295484111784305 1103 18-19 0.633528227776452 1103 20-21 0.6228006907581758 1103 22-23 0.5741128902059387 1103 24-25 0.7214696066426853 1103 26-27 0.6289108543981783 1103 28-29 0.6395970686670225 1103 30-31 0.6320122688568119 1103 32-33 0.6897516262939121 1103 34-35 0.7110526611365842 1103 36-37 0.7495393808151647 1103 38-39 0.703817173523646 1103 40-41 0.7293342619011938 1103 42-43 0.7368904362361519 1103 44-45 0.6706866893757208 1103 46-47 0.5919240266901156 1103 48-49 0.4557702312878007 1103 50-51 0.38606343814320354 1103 52-53 0.39613709804525854 1103 54-55 0.5708413965346182 1103 56-57 0.6539351812268279 1103 58-59 0.5628634605870175 1103 60-61 0.6604751628364056 1103 62-63 0.5641578971078758 1103 64-65 0.48745020069914347 1103 66-67 0.5309280001739936 1103 68-69 0.4782975207435598 1103 70-71 0.4633715060658048 1103 72-73 0.5217903450359387 1103 74-75 0.4395335400932936 1103 76-77 0.47748019717738543 1103 78-79 0.4646415698701247 1103 80-81 0.39109769265598615 1103 82-83 0.4527256778346569 1103 84-85 0.6642427911475473 1103 86-87 0.8218921376155812 1103 88-89 0.751095535162257 1103 90-91 0.6261036547590209 1103 92-93 0.7048198379919057 1103 94-95 0.6407841403601822 1103 96-97 0.6508703316107365 1103 98-99 0.6072332709805188 1103 100-101 0.8695300905142851 1104 1 0.21583101836722207 1104 2 0.2128643230138323 1104 3 0.25601395668591564 1104 4 0.21866148256812323 1104 5 0.28332102248967317 1104 6 0.2825802305580396 1104 7 0.2900639507535203 1104 8 0.21734452299880047 1104 9 0.41045653588130193 1104 10-11 0.28495866784116686 1104 12-13 0.361941859064693 1104 14-15 0.3804418216423997 1104 16-17 0.46284444472007635 1104 18-19 0.5254417904264557 1104 20-21 0.43421868328237423 1104 22-23 0.3316361967916137 1104 24-25 0.48433797488532804 1104 26-27 0.44110832038028747 1104 28-29 0.36618981471861645 1104 30-31 0.43322889662036346 1104 32-33 0.4612092557725873 1104 34-35 0.43531263176402746 1104 36-37 0.4753818189817949 1104 38-39 0.5003898894123893 1104 40-41 0.5400261727824898 1104 42-43 0.4239617140739895 1104 44-45 0.5573511545120411 1104 46-47 0.6639166562675811 1104 48-49 0.47418993705421997 1104 50-51 0.4921233385851096 1104 52-53 0.5743154633995715 1104 54-55 0.4755687595810869 1104 56-57 0.4691312914802239 1104 58-59 0.48754054594900964 1104 60-61 0.46613648279497255 1104 62-63 0.4671553183067303 1104 64-65 0.5084621143652797 1104 66-67 0.5760144478730638 1104 68-69 0.5529037619880057 1104 70-71 0.4770409867398513 1104 72-73 0.4289817285848585 1104 74-75 0.4362272460685652 1104 76-77 0.2736826984545573 1104 78-79 0.2190361631373925 1104 80-81 0.2471940500640919 1104 82-83 0.2863069185381306 1104 84-85 0.4277017622425845 1104 86-87 0.5684593026372156 1104 88-89 0.578652438606337 1104 90-91 0.5098492105372365 1104 92-93 0.6979003058076962 1104 94-95 0.686082979288134 1104 96-97 0.7453804299551692 1104 98-99 0.6695466936357697 1104 100-101 0.9806956126674216 1105 1 0.14884756190307158 1105 2 0.21332632422513598 1105 3 0.23243938334195136 1105 4 0.20351182800323642 1105 5 0.2730304899927276 1105 6 0.28340182348446774 1105 7 0.3187708153871611 1105 8 0.35144998995135524 1105 9 0.3345305524605209 1105 10-11 0.3068967745073792 1105 12-13 0.4579962899436154 1105 14-15 0.4905536935906767 1105 16-17 0.5447851324762993 1105 18-19 0.48533955238508497 1105 20-21 0.33913470981246263 1105 22-23 0.37833226255241925 1105 24-25 0.3563013132124766 1105 26-27 0.2747286860609748 1105 28-29 0.34246104789114185 1105 30-31 0.40174133613041363 1105 32-33 0.4202487010374938 1105 34-35 0.3383377897457365 1105 36-37 0.4904791103201305 1105 38-39 0.4556846688913865 1105 40-41 0.28222231430778066 1105 42-43 0.22737788170675088 1105 44-45 0.47441730316892716 1105 46-47 0.4591020661117753 1105 48-49 0.49666546187971505 1105 50-51 0.4331666318520817 1105 52-53 0.456972948120395 1105 54-55 0.41040457769625505 1105 56-57 0.38489183490857215 1105 58-59 0.4152474351423976 1105 60-61 0.5121405893694302 1105 62-63 0.44533850433566613 1105 64-65 0.4445780511827877 1105 66-67 0.3836584826589302 1105 68-69 0.3410876755414236 1105 70-71 0.18096389602218466 1105 72-73 0.5123033226697586 1105 74-75 0.4779229712366977 1105 76-77 0.2628568330595584 1105 78-79 0.2501057445976578 1105 80-81 0.3100841387247648 1105 82-83 0.3933196289004428 1105 84-85 0.42050181859097435 1105 86-87 0.438351629014889 1105 88-89 0.512140019485507 1105 90-91 0.6286933456009969 1105 92-93 0.8907815251572515 1105 94-95 0.6825219323387053 1105 96-97 0.7451642578738493 1105 98-99 0.9903069057392777 1105 100-101 0.9943203620306029 1106 1 0.15205054053381062 1106 2 0.2566100662693884 1106 3 0.29064484241308364 1106 4 0.31192638214435675 1106 5 0.18499622129762372 1106 6 0.2477026763400758 1106 7 0.151430549007749 1106 8 0.18574765970841867 1106 9 0.2525304606008234 1106 10-11 0.277887961231869 1106 12-13 0.27193401135234296 1106 14-15 0.30812649982909335 1106 16-17 0.5207308018056054 1106 18-19 0.40250781033443417 1106 20-21 0.3498299666300184 1106 22-23 0.378316724095761 1106 24-25 0.4492322523133794 1106 26-27 0.429264235878982 1106 28-29 0.2253520417331103 1106 30-31 0.3255356033082393 1106 32-33 0.39722116529276263 1106 34-35 0.3079209180895006 1106 36-37 0.21394131153903118 1106 38-39 0.2866877288248091 1106 40-41 0.38663263102036893 1106 42-43 0.5297206011057582 1106 44-45 0.5632147842936988 1106 46-47 0.5194445199859743 1106 48-49 0.6480066020688184 1106 50-51 0.5247001381345271 1106 52-53 0.47364708314821513 1106 54-55 0.4034444916497648 1106 56-57 0.49926664396264187 1106 58-59 0.6491119475696152 1106 60-61 0.6688107346699965 1106 62-63 0.5417125826252658 1106 64-65 0.3603504758606917 1106 66-67 0.3753390101621079 1106 68-69 0.5193576466284568 1106 70-71 0.39878346666263553 1106 72-73 0.3145016258245903 1106 74-75 0.4208221136281445 1106 76-77 0.5100729928716703 1106 78-79 0.5467940653223735 1106 80-81 0.6921116056889751 1106 82-83 0.7420750327986312 1106 84-85 0.7092272515483629 1106 86-87 0.699819295657413 1106 88-89 0.8025454338150269 1106 90-91 0.7303813475588434 1106 92-93 0.7791463973682973 1106 94-95 0.8685663874061724 1106 96-97 0.927165286611082 1106 98-99 1.0744589595919116 1106 100-101 1.434108271237573 1107 1 0.163554294969515 1107 2 0.09907777875096357 1107 3 0.14708207308850518 1107 4 0.14192069312918676 1107 5 0.15249968656791424 1107 6 0.19770220487473011 1107 7 0.13421263458184995 1107 8 0.15474567012466167 1107 9 0.15271653225726567 1107 10-11 0.2627939981884637 1107 12-13 0.2953941269556424 1107 14-15 0.3694272098559068 1107 16-17 0.2765298255580291 1107 18-19 0.38874573689270164 1107 20-21 0.358589006978022 1107 22-23 0.26656607897401585 1107 24-25 0.3878655371381683 1107 26-27 0.4146362377522692 1107 28-29 0.3599208238124021 1107 30-31 0.33762240322148784 1107 32-33 0.44616791171040404 1107 34-35 0.3690888320439356 1107 36-37 0.401893328939245 1107 38-39 0.4527071783418748 1107 40-41 0.3188862039419149 1107 42-43 0.5490640384652963 1107 44-45 0.7672044812043382 1107 46-47 0.6409254240679232 1107 48-49 0.5631019795082537 1107 50-51 0.47169328931460797 1107 52-53 0.45058535576205117 1107 54-55 0.4450371644496727 1107 56-57 0.58538136091056 1107 58-59 0.5057526941192734 1107 60-61 0.45345007939602766 1107 62-63 0.3329485133135748 1107 64-65 0.3536556679513865 1107 66-67 0.34982723452932873 1107 68-69 0.39297586027769427 1107 70-71 0.427029741300629 1107 72-73 0.36837518510507294 1107 74-75 0.40776330932711957 1107 76-77 0.435684404847521 1107 78-79 0.4772505695011269 1107 80-81 0.5452462216175924 1107 82-83 0.5828753295074875 1107 84-85 0.5439998638121217 1107 86-87 0.41941056663369025 1107 88-89 0.5078230168950775 1107 90-91 0.5963138902971785 1107 92-93 0.7742415862216028 1107 94-95 0.5984607477377075 1107 96-97 0.684199549569307 1107 98-99 0.825704413880203 1107 100-101 1.1170486120127272 1108 1 0.17526541603929502 1108 2 0.16069521454058489 1108 3 0.08736185643207506 1108 4 0.2025252029320228 1108 5 0.11192271064761172 1108 6 0.23792509469678436 1108 7 0.26824528795922475 1108 8 0.312843268793884 1108 9 0.24517634256301335 1108 10-11 0.28822457241653154 1108 12-13 0.21605071692209776 1108 14-15 0.39592004237871237 1108 16-17 0.39947262221117796 1108 18-19 0.2520007286768049 1108 20-21 0.31617543994786246 1108 22-23 0.3994631671691167 1108 24-25 0.3818838795357351 1108 26-27 0.42315850921458775 1108 28-29 0.33699835944692325 1108 30-31 0.4176873558901377 1108 32-33 0.4899851442693617 1108 34-35 0.7526442990779643 1108 36-37 0.5442336351868349 1108 38-39 0.4808132234399025 1108 40-41 0.44966361656840803 1108 42-43 0.6803359267954008 1108 44-45 0.49395259689459436 1108 46-47 0.49023366523744016 1108 48-49 0.5593377399911361 1108 50-51 0.5658172581771623 1108 52-53 0.6531504974610343 1108 54-55 0.6953562107777884 1108 56-57 0.7602623179020114 1108 58-59 0.6919886113590294 1108 60-61 0.5313478683865327 1108 62-63 0.6520229654483245 1108 64-65 0.6815287907879153 1108 66-67 0.6941397726811829 1108 68-69 0.711776909995443 1108 70-71 0.8482617211767334 1108 72-73 0.9143777928016448 1108 74-75 0.9984360913557744 1108 76-77 0.8518413811735428 1108 78-79 0.9699228775707844 1108 80-81 0.9902206425853883 1108 82-83 1.024318802059362 1108 84-85 1.1799866759491557 1108 86-87 1.2694463440831996 1108 88-89 1.237373853146856 1108 90-91 1.3348107026001905 1108 92-93 1.4052451436803715 1108 94-95 1.4108375322302145 1108 96-97 1.5174692580568596 1108 98-99 1.661112470445456 1108 100-101 2.0797520040468953 1109 1 0.04074633749579348 1109 2 0.041501435125809394 1109 3 0.005393584001460283 1109 4 0.21856399543199245 1109 5 0.16136107020519574 1109 6 0.10836008333474467 1109 7 0.025482533928659734 1109 8 0.1839729038083746 1109 9 0.16719555572791478 1109 10-11 0.15620541310019576 1109 12-13 0.2415574318370659 1109 14-15 0.13181306683672744 1109 16-17 0.11020356251466978 1109 18-19 0.06318615079563017 1109 20-21 0.37966288784938484 1109 22-23 0.33585052137414806 1109 24-25 0.17454135963869533 1109 26-27 0.22040254010212124 1109 28-29 0.2779103385967616 1109 30-31 0.26958632522877224 1109 32-33 0.1318155105472414 1109 34-35 0.2150889696863416 1109 36-37 0.22604251589074664 1109 38-39 0.3370187310702377 1109 40-41 0.3055002615879516 1109 42-43 0.286822793132508 1109 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2316 54-55 -2.5362934859400355 2316 56-57 -2.3245958941603106 2316 58-59 -2.2066030998161708 2316 60-61 -2.0570597993358533 2316 62-63 -2.319868725251805 2316 64-65 -2.731320303399677 2316 66-67 -2.708338110059856 2316 68-69 -2.8592432202686098 2316 70-71 -2.758268379074977 2316 72-73 -2.6701878503399996 2316 74-75 -2.8116353963138927 2316 76-77 -2.741580584843934 2316 78-79 -2.5432537606058503 2316 80-81 -2.3973865565700336 2316 82-83 -2.541568099454043 2316 84-85 -2.7865041449031374 2316 86-87 -2.844524996911918 2316 88-89 -3.026322082970932 2316 90-91 -3.2585485632811135 2316 92-93 -3.43845397787074 2316 94-95 -3.5800590549794933 2316 96-97 -3.858539177061914 2316 98-99 -4.270226028085133 2316 100-101 -4.858322715486548 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8370.0 3 2602.0 4 2583.0 5 2762.0 6 3327.0 7 3991.0 8 4818.0 9 5884.0 10 6960.0 11 8439.0 12 10604.0 13 14233.0 14 14143.0 15 13508.0 16 16832.0 17 21592.0 18 19165.0 19 14165.0 20 13951.0 21 15194.0 22 16043.0 23 17850.0 24 20296.0 25 23024.0 26 26446.0 27 30619.0 28 35921.0 29 42409.0 30 50181.0 31 60784.0 32 74670.0 33 95386.0 34 127477.0 35 180445.0 36 275707.0 37 419974.0 38 378892.0 39 60523.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.71756984424903 19.665909820489208 15.980935911765325 21.635584423496443 2 15.129765222377825 21.021470220371217 39.78286554614774 24.065899011103223 3 19.184471905003804 25.34214216686453 30.572527403648706 24.900858524482963 4 12.883365839441192 17.902857808506667 38.121407388936944 31.092368963115195 5 13.82716037831182 37.44109930498116 33.891338276188584 14.840402040518438 6 30.02797921063266 36.65800443027554 18.15589780431952 15.158118554772274 7 27.087421466905354 33.807950535903146 21.093997407606818 18.01063058958468 8 26.022312831491863 33.957162095222685 20.15905528532331 19.861469787962147 9 26.10710747327365 17.681957621418125 21.0695404293617 35.14139447594652 10-11 24.6876255613572 26.984296264697257 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21.873785417148003 36-37 22.519721793674925 26.277486177586866 29.244475030343033 21.95831699839518 38-39 22.266625276732523 26.539932317588004 29.255218527337686 21.93822387834179 40-41 22.40446939762591 26.552117563404988 29.154818642723264 21.888594396245843 42-43 22.11091655529051 26.710305443239513 28.75107381654608 22.427704184923897 44-45 22.35362467073659 26.761147951845288 28.58415324074161 22.30107413667651 46-47 22.62895628275973 26.88306545895054 28.110043321274187 22.377934937015546 48-49 22.610590733137887 26.864376084294882 28.160629245364845 22.364403937202386 50-51 22.339168394497204 27.139732644175336 28.234247285570785 22.286851675756676 52-53 22.544390143927316 27.292414513115677 27.835435353279664 22.32775998967734 54-55 22.351957457788917 26.97424915902144 28.072468216251227 22.601325166938416 56-57 22.503080122659835 27.396363622694135 27.7586715672961 22.341884687349932 58-59 22.748121731530947 26.938340169807685 27.903786608028042 22.409751490633326 60-61 22.530542073524504 26.87850393558429 28.334892007698592 22.256061983192616 62-63 22.489458635365597 26.910769204740276 28.31677012211628 22.283002037777845 64-65 22.334881239076804 27.08125153527098 28.1795476636261 22.404319562026124 66-67 22.315987302805336 27.13907810392196 27.831560456260178 22.71337413701253 68-69 22.242216185894648 27.073783368355425 28.092684011730558 22.59131643401937 70-71 22.456129297382287 26.99803180149877 27.802825629649554 22.743013271469398 72-73 22.401380147845444 26.76027500466028 28.0891303954338 22.749214452060478 74-75 22.24819170224574 27.40145538262854 27.637836939507622 22.712515975618096 76-77 22.4380845209716 26.983578593297615 27.737537642134093 22.840799243596695 78-79 22.39166175775008 27.095927240104885 27.785094484993756 22.727316517151287 80-81 22.397171119099646 27.271328211273232 27.572354972862477 22.759145696764644 82-83 22.415353770060037 27.168006587277194 27.818509609735788 22.598130032926985 84-85 22.412036989164022 27.0568101593294 27.652215561431927 22.87893729007465 86-87 22.408779278458926 27.067648147386603 27.786228329387434 22.737344244767037 88-89 22.489742467664506 26.93884870274943 27.599283841160894 22.97212498842517 90-91 22.349627988065716 26.982894244151623 27.820400023599657 22.847077744183004 92-93 22.542757847048723 27.078935466187236 27.714508565673928 22.66379812109011 94-95 22.418389628682714 27.014503887316366 27.62069157630191 22.94641490769901 96-97 22.25052678295226 26.851685564407617 27.776881915054663 23.12090573758546 98-99 22.31629174418293 26.931771113220528 27.46337165539948 23.28856548719706 100-101 22.65272341968661 26.377738184797376 27.945742418911752 23.023795976604262 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2067.0 1 1188.5 2 344.0 3 373.0 4 329.5 5 323.5 6 569.5 7 1181.0 8 2223.0 9 2956.0 10 2694.5 11 2359.0 12 2496.5 13 2758.0 14 3017.0 15 3307.0 16 3539.0 17 3659.5 18 3792.5 19 3861.5 20 4107.5 21 4602.5 22 5333.0 23 6384.5 24 7610.5 25 9262.5 26 12078.0 27 15666.0 28 18886.0 29 22035.5 30 25353.0 31 29348.5 32 33459.5 33 37191.5 34 41796.0 35 46206.0 36 50458.5 37 55063.0 38 58077.5 39 59163.0 40 60691.5 41 63905.5 42 66611.5 43 68773.5 44 72287.0 45 75215.5 46 76315.5 47 81668.0 48 96877.5 49 97427.5 50 83267.0 51 76949.0 52 73297.5 53 71159.5 54 68973.5 55 64052.5 56 59157.5 57 54021.5 58 48569.5 59 43069.0 60 36249.5 61 29135.0 62 22763.5 63 17374.5 64 12910.0 65 9560.5 66 6981.0 67 5319.0 68 3999.5 69 2874.5 70 2068.5 71 1359.5 72 893.5 73 614.5 74 423.0 75 294.0 76 195.0 77 110.5 78 68.0 79 42.5 80 35.0 81 28.0 82 18.0 83 16.0 84 16.0 85 11.0 86 6.5 87 6.5 88 6.0 89 5.5 90 4.0 91 2.5 92 3.5 93 2.5 94 1.0 95 0.5 96 2.5 97 3.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27703743790842345 2 0.08622369651502045 3 0.0901493282262734 4 0.10449657745668602 5 0.10687999670994676 6 0.08257846706885699 7 0.09300008458801663 8 0.08926138772015667 9 0.09370109025074039 10-11 0.08804631123810219 12-13 0.09148123898544852 14-15 0.10015034234779883 16-17 0.08888751803337068 18-19 0.09977647266101282 20-21 0.1021131582034253 22-23 0.11440412415651494 24-25 0.1270923266518147 26-27 0.13008328414610268 28-29 0.12202171902477962 30-31 0.11400688761430483 32-33 0.10938025024032812 34-35 0.38020210460593434 36-37 0.5814140966630729 38-39 0.5632113162876796 40-41 0.526385152139259 42-43 0.5542150769493915 44-45 0.5751517794094074 46-47 0.5643329253480376 48-49 0.5858771660490807 50-51 0.5774417312409716 52-53 0.5806429904340767 54-55 0.5702447397703412 56-57 0.5430924537675081 58-59 0.5371105387789322 60-61 0.48147405601409116 62-63 0.5596361874077885 64-65 0.5942425002909173 66-67 0.6081925129791199 68-69 0.6089402523526919 70-71 0.6059025611475556 72-73 0.5965324521224816 74-75 0.5941490328692208 76-77 0.5769977609879132 78-79 0.553911307828878 80-81 0.5483266293825122 82-83 0.562907547167166 84-85 0.5720673544934229 86-87 0.5629309140225901 88-89 0.5758527850721311 90-91 0.590410336001361 92-93 0.5984251674118357 94-95 0.5982615994238668 96-97 0.5944995357005828 98-99 0.5986588359660769 100-101 0.574894743999742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2139783.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.92022343771356 #Duplication Level Percentage of deduplicated Percentage of total 1 79.00418720472732 47.33948549822212 2 12.373132197967053 14.828016918531073 3 3.856442286137833 6.9323665038007745 4 1.6050824317215024 3.8470759177880414 5 0.8526616637953566 2.5545838705695183 6 0.49550088936282366 1.7814314402523705 7 0.35023237500638826 1.469020151585271 8 0.23401475252108847 1.1217773007027891 9 0.1763286081091354 0.9509084636724381 >10 0.9534710288016378 10.310757944769334 >50 0.06450750118584846 2.6359764876685685 >100 0.03146694825152976 3.3399855442572846 >500 0.0016424831753243658 0.6906128770158401 >1k 0.0011732022680887993 1.4441663820061523 >5k 1.5642696907850658E-4 0.753834699158493 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8865 0.41429434666973236 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7220 0.33741739232436185 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4538 0.21207757982935654 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3719 0.17380267064464014 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2987 0.13959359430372145 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2584 0.12075990883187687 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2401 0.11220763974664721 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.673371084824957E-5 0.0 4 0.0 0.0 0.0 4.673371084824957E-5 0.0 5 0.0 0.0 0.0 4.673371084824957E-5 0.0 6 0.0 0.0 0.0 4.673371084824957E-5 0.0 7 0.0 0.0 0.0 4.673371084824957E-5 0.0 8 0.0 0.0 0.0 4.673371084824957E-5 0.0 9 0.0 0.0 0.0 9.346742169649914E-5 0.0 10-11 0.0 0.0 0.0 9.346742169649914E-5 0.0 12-13 0.0 0.0 0.0 1.6356798796887347E-4 9.346742169649914E-5 14-15 0.0 0.0 0.0 1.8693484339299827E-4 9.346742169649914E-5 16-17 0.0 0.0 0.0 1.8693484339299827E-4 9.346742169649914E-5 18-19 0.0 0.0 0.0 1.8693484339299827E-4 9.346742169649914E-5 20-21 0.0 0.0 0.0 3.7386968678599654E-4 9.346742169649914E-5 22-23 0.0 0.0 0.0 4.907039639066204E-4 9.346742169649914E-5 24-25 0.0 0.0 0.0 5.841713856031196E-4 9.346742169649914E-5 26-27 0.0 0.0 0.0 8.64573650692617E-4 1.1683427712062392E-4 28-29 0.0 0.0 0.0 0.002056283277322981 1.402011325447487E-4 30-31 0.0 0.0 0.0 0.004556536807704332 1.402011325447487E-4 32-33 2.3366855424124784E-5 0.0 0.0 0.009159807326256915 1.402011325447487E-4 34-35 4.673371084824957E-5 0.0 0.0 0.01647363307400797 1.402011325447487E-4 36-37 4.673371084824957E-5 0.0 0.0 0.028904800159642356 1.402011325447487E-4 38-39 4.673371084824957E-5 0.0 0.0 0.048930195258117294 1.6356798796887347E-4 40-41 4.673371084824957E-5 0.0 0.0 0.07253071923648333 1.8693484339299827E-4 42-43 4.673371084824957E-5 0.0 0.0 0.09860812988980658 1.8693484339299827E-4 44-45 4.673371084824957E-5 0.0 0.0 0.13048052068831278 2.1030169881712307E-4 46-47 4.673371084824957E-5 0.0 0.0 0.17176975422274127 2.3366855424124784E-4 48-49 4.673371084824957E-5 0.0 0.0 0.21911100331201808 2.3366855424124784E-4 50-51 4.673371084824957E-5 0.0 0.0 0.2670130569314739 2.3366855424124784E-4 52-53 4.673371084824957E-5 0.0 0.0 0.31874727484048615 2.804022650894974E-4 54-55 4.673371084824957E-5 0.0 0.0 0.380692808569841 2.804022650894974E-4 56-57 4.673371084824957E-5 0.0 0.0 0.45859790455387295 2.804022650894974E-4 58-59 4.673371084824957E-5 0.0 0.0 0.5457562752858585 3.0376912051362215E-4 60-61 4.673371084824957E-5 0.0 0.0 0.6435699320912447 3.2713597593774695E-4 62-63 4.673371084824957E-5 0.0 0.0 0.742692132800382 3.2713597593774695E-4 64-65 4.673371084824957E-5 0.0 0.0 0.8536379623541266 3.2713597593774695E-4 66-67 4.673371084824957E-5 0.0 0.0 0.9844923527292253 3.2713597593774695E-4 68-69 4.673371084824957E-5 0.0 0.0 1.1356759073233127 3.2713597593774695E-4 70-71 4.673371084824957E-5 0.0 0.0 1.3041275680758282 3.5050283136187175E-4 72-73 4.673371084824957E-5 0.0 0.0 1.4786312443831922 3.9723654221012134E-4 74-75 4.673371084824957E-5 0.0 0.0 1.668486944704206 4.206033976342461E-4 76-77 4.673371084824957E-5 0.0 0.0 1.8743956747015935 4.206033976342461E-4 78-79 4.673371084824957E-5 0.0 0.0 2.11864941444997 4.206033976342461E-4 80-81 9.346742169649914E-5 0.0 0.0 2.3947755449968526 4.206033976342461E-4 82-83 9.346742169649914E-5 0.0 0.0 2.6732383610861477 4.673371084824957E-4 84-85 9.346742169649914E-5 0.0 0.0 2.9717499391293414 4.673371084824957E-4 86-87 9.346742169649914E-5 0.0 0.0 3.291408521331369 4.673371084824957E-4 88-89 1.402011325447487E-4 0.0 0.0 3.6361631062589055 4.673371084824957E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 410 0.0 27.707443 3 GAGTACT 7390 0.0 23.506098 12-13 GTACTTT 8030 0.0 21.574194 14-15 GTATCAA 19955 0.0 21.147041 1 AGTACTT 7805 0.0 20.527906 12-13 GGTATCA 15610 0.0 20.350882 1 TTGCGTT 585 0.0 20.228014 4 GTACATG 9860 0.0 19.667889 1 TACATGG 9875 0.0 19.268948 2 TATCAAC 22435 0.0 18.60851 2 CAACGCA 22395 0.0 18.599474 5 ATCAACG 22375 0.0 18.594944 3 TCAACGC 22415 0.0 18.582878 4 ACATGGG 9930 0.0 18.399546 3 AACGCAG 22720 0.0 18.33213 6 CTTTGCG 630 0.0 18.031828 2 ACTTTTT 9760 0.0 17.96937 16-17 ACGCAGA 24325 0.0 16.694485 7 CGCAGAG 24225 0.0 16.52972 8 AGAGTAC 16780 0.0 16.445444 10-11 >>END_MODULE