FastQCFastQC Report
Thu 26 May 2016
SRR938916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139783
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90070.42093053361018384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79610.3720470720629148No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50530.23614544091620504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40520.18936499635710724No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26660.12459207312143335No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25640.11982523461491189No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24750.11566593434941766No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21910.1023935604685148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT75900.022.60206412-13
GTACTTT77750.021.97271314-15
GTATCAA210600.020.0445291
TTTGCGT4100.019.6759873
AGTACTT79750.019.27950712-13
GTACATG100650.019.124411
TACATGG100300.018.9247652
GGTATCA167500.018.3470131
ACTTTTT93650.018.34329216-17
TATCAAC237650.017.691612
ACATGGG100400.017.6770323
ATCAACG237650.017.6716423
TCAACGC238650.017.6572464
CAACGCA239050.017.607855
AACGCAG241900.017.4003986
CTTTGCG4851.8189894E-1216.6333082
GTGTAGC13400.016.3558651
AGAGTAC176900.016.3101310-11
TACTTTT93000.016.25204814-15
CATGGGG49900.016.1666414
Produced by FastQC (version 0.11.5)