##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938916_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2139783 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16204026296124 33.0 31.0 34.0 30.0 34.0 2 32.399785398799786 34.0 31.0 34.0 31.0 34.0 3 32.48224936827707 34.0 31.0 34.0 30.0 34.0 4 36.054129320589986 37.0 35.0 37.0 35.0 37.0 5 35.97419317753249 37.0 35.0 37.0 35.0 37.0 6 36.0143187416668 37.0 35.0 37.0 35.0 37.0 7 35.96466744525029 37.0 35.0 37.0 35.0 37.0 8 35.93361195971741 37.0 35.0 37.0 35.0 37.0 9 37.607153155249854 39.0 37.0 39.0 35.0 39.0 10-11 37.53230280827542 39.0 37.0 39.0 35.0 39.0 12-13 37.461252379330055 39.0 37.0 39.0 34.0 39.0 14-15 38.69998383013605 40.0 38.0 41.0 34.0 41.0 16-17 38.68510802263594 40.0 38.0 41.0 34.0 41.0 18-19 38.81208912305594 40.0 38.0 41.0 34.5 41.0 20-21 38.81884120959929 40.0 38.0 41.0 34.0 41.0 22-23 38.76214013290132 40.0 38.0 41.0 34.0 41.0 24-25 38.697008995771995 40.0 38.0 41.0 34.0 41.0 26-27 38.54263820209806 40.0 38.0 41.0 34.0 41.0 28-29 38.361528482093746 40.0 38.0 41.0 34.0 41.0 30-31 38.19296886646917 40.0 38.0 41.0 33.5 41.0 32-33 38.03244184106519 40.0 37.0 41.0 33.0 41.0 34-35 38.004873391367255 40.0 37.0 41.0 33.0 41.0 36-37 37.899253802838885 40.0 37.0 41.0 33.0 41.0 38-39 37.772131099275015 40.0 37.0 41.0 33.0 41.0 40-41 37.614405759836394 40.0 37.0 41.0 33.0 41.0 42-43 37.44587932514652 40.0 37.0 41.0 32.0 41.0 44-45 37.18355062172192 40.0 36.5 41.0 31.0 41.0 46-47 36.861445296088434 40.0 36.0 41.0 31.0 41.0 48-49 36.544323887048364 39.5 36.0 41.0 30.0 41.0 50-51 36.267924130624465 39.0 35.5 41.0 30.0 41.0 52-53 36.025285040585885 39.0 35.0 40.5 29.0 41.0 54-55 35.76948877526366 39.0 35.0 40.0 28.0 41.0 56-57 35.48980363896713 38.0 35.0 40.0 28.0 41.0 58-59 35.182435789049634 38.0 34.0 40.0 27.5 41.0 60-61 34.76786290946325 38.0 34.0 40.0 26.0 41.0 62-63 34.44326691071011 37.0 34.0 40.0 26.0 41.0 64-65 34.16263892179721 37.0 33.5 39.5 26.0 41.0 66-67 33.696562922501954 36.0 33.0 39.0 25.5 40.5 68-69 33.26894619688071 36.0 33.0 39.0 24.0 40.0 70-71 32.79898358852276 35.0 32.0 38.5 23.0 40.0 72-73 32.27068772861547 35.0 31.5 37.5 22.0 39.5 74-75 31.794529398541815 35.0 31.0 37.0 20.5 39.0 76-77 29.833085644665836 33.0 28.5 35.0 18.0 37.5 78-79 30.79853564590428 34.0 30.5 36.0 18.5 38.0 80-81 30.78903888852281 34.0 31.0 35.5 18.0 37.0 82-83 30.45569200241333 34.0 30.5 35.0 17.5 37.0 84-85 30.0321364362648 34.0 30.0 35.0 10.5 36.5 86-87 29.441118795690965 34.0 29.5 35.0 6.5 36.0 88-89 28.84634469943915 34.0 29.0 35.0 2.0 35.5 90-91 28.35265842377475 33.5 28.5 35.0 2.0 35.0 92-93 27.96794488039208 33.0 27.0 35.0 2.0 35.0 94-95 27.542691011191323 33.0 27.0 35.0 2.0 35.0 96-97 26.87221437874775 33.0 25.5 35.0 2.0 35.0 98-99 25.901345136399343 32.5 24.5 34.0 2.0 35.0 100-101 21.39322678981934 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07854988107340688 1101 2 0.07667376660808856 1101 3 0.035502938912834736 1101 4 0.09213653427788415 1101 5 0.1312138821251736 1101 6 0.17111998044599375 1101 7 0.09480508374518593 1101 8 0.20300944009087374 1101 9 0.2420461920795205 1101 10-11 0.22089213820878228 1101 12-13 0.2244257235838205 1101 14-15 0.19245539614693286 1101 16-17 0.14685912717867922 1101 18-19 0.2263942207826588 1101 20-21 0.18153430001223825 1101 22-23 0.18839870605691544 1101 24-25 0.18602735764994094 1101 26-27 0.23881433900262294 1101 28-29 0.27195244808620345 1101 30-31 0.1934122334978241 1101 32-33 0.2602666524462407 1101 34-35 0.37280778297628103 1101 36-37 0.29722896919550834 1101 38-39 0.399930305983176 1101 40-41 0.4033044874974152 1101 42-43 0.40891458158294824 1101 44-45 0.43005171253002317 1101 46-47 0.5139801602835519 1101 48-49 0.30008492188543556 1101 50-51 0.29279331454738866 1101 52-53 0.2744032711925328 1101 54-55 0.21433182704743814 1101 56-57 0.15133748828519344 1101 58-59 0.2447511778462541 1101 60-61 0.40224493446564935 1101 62-63 0.32578787966026823 1101 64-65 0.20396493765132107 1101 66-67 0.48144958070118804 1101 68-69 0.4881965104373762 1101 70-71 0.5230906851708781 1101 72-73 0.36244868731022706 1101 74-75 0.3097559544104662 1101 76-77 0.10062974090958932 1101 78-79 0.278419728600376 1101 80-81 0.43588739099498497 1101 82-83 0.5042369630566661 1101 84-85 0.4704365468524365 1101 86-87 0.5232239709155806 1101 88-89 0.40256260722868475 1101 90-91 0.5529308668636155 1101 92-93 0.6248873871486538 1101 94-95 0.5863786859847764 1101 96-97 0.5339880675385551 1101 98-99 0.42662523583060974 1101 100-101 1.2168610787123093 1102 1 0.15144011099087606 1102 2 0.1088358932036897 1102 3 0.07441642376986835 1102 4 0.025203855165969458 1102 5 0.05990515729295964 1102 6 0.10350629067478678 1102 7 0.09936160433999675 1102 8 0.14700160071350865 1102 9 0.10469917273486828 1102 10-11 0.11049462349819805 1102 12-13 0.1344251595998358 1102 14-15 0.1714592124385419 1102 16-17 0.22841332603942988 1102 18-19 0.1163195869022573 1102 20-21 0.15538447766719088 1102 22-23 0.0798139298645637 1102 24-25 0.21021945059450076 1102 26-27 0.20151267336992618 1102 28-29 0.21367574814448176 1102 30-31 0.05391197030530037 1102 32-33 0.005186801713996658 1102 34-35 0.007080384634384984 1102 36-37 0.04475801437062188 1102 38-39 0.1168072916767926 1102 40-41 -5.656175916044504E-4 1102 42-43 -0.005939346565490666 1102 44-45 0.1070040370840104 1102 46-47 -0.028078475251206214 1102 48-49 -0.07717124903931705 1102 50-51 -0.03771751394508982 1102 52-53 -0.11356013564671485 1102 54-55 -0.22172202402318675 1102 56-57 -0.19964760373819956 1102 58-59 -0.3298079746738125 1102 60-61 -0.4046293253053648 1102 62-63 -0.20804810439778265 1102 64-65 -0.2785524988534789 1102 66-67 -0.34980124177545946 1102 68-69 -0.3018120433196927 1102 70-71 -0.20577753997152826 1102 72-73 -0.2709401044480799 1102 74-75 -0.20260962940695393 1102 76-77 -0.23696825130743093 1102 78-79 -0.34249632238377714 1102 80-81 -0.2192425133157343 1102 82-83 -0.14821683725540424 1102 84-85 -0.09399368832888655 1102 86-87 -0.01387607044324568 1102 88-89 0.165892133670134 1102 90-91 0.060836982329938394 1102 92-93 0.10356667765680072 1102 94-95 0.19498813647638258 1102 96-97 -0.14740586587373627 1102 98-99 -0.17452952137428568 1102 100-101 0.4974097411302978 1103 1 0.09831585197790105 1103 2 0.13972937008114883 1103 3 0.14291338109557472 1103 4 0.03544341714833621 1103 5 0.05851104732556678 1103 6 0.07376933930977714 1103 7 0.09360475023935066 1103 8 0.16347318570642244 1103 9 0.11147290704703039 1103 10-11 0.09474211808343114 1103 12-13 0.18177147713451092 1103 14-15 0.16431152862924847 1103 16-17 0.15849086198403484 1103 18-19 0.16000068389575972 1103 20-21 0.11744008461347022 1103 22-23 0.12590086234465048 1103 24-25 0.06461110786556645 1103 26-27 0.12600814614425104 1103 28-29 0.26804884852320754 1103 30-31 0.13379447143699252 1103 32-33 0.11169914626488264 1103 34-35 0.16730134286357412 1103 36-37 0.10422324784538262 1103 38-39 0.1432091571130769 1103 40-41 0.2220936600579293 1103 42-43 0.24309461723357373 1103 44-45 0.2443737756661264 1103 46-47 0.4079152416474727 1103 48-49 0.2747147591575825 1103 50-51 0.23403950510572002 1103 52-53 0.2508817658161817 1103 54-55 0.3713332933230973 1103 56-57 0.43692198601389975 1103 58-59 0.30395277637422424 1103 60-61 0.43144466421092176 1103 62-63 0.3982039281911156 1103 64-65 0.45177414931252713 1103 66-67 0.4316925224526713 1103 68-69 0.5767494976146494 1103 70-71 0.4557534130186198 1103 72-73 0.28368955902548265 1103 74-75 0.3955567996752656 1103 76-77 0.35015823265819535 1103 78-79 0.5700238629227243 1103 80-81 0.6312887480187328 1103 82-83 0.678690703497125 1103 84-85 0.580009745633415 1103 86-87 0.3679863480071788 1103 88-89 0.6057143811347281 1103 90-91 0.7550957797495919 1103 92-93 0.8098947185269516 1103 94-95 0.6268644257352207 1103 96-97 0.35838215068501 1103 98-99 0.7093821215761089 1103 100-101 1.4506932332750146 1104 1 0.05695577663664153 1104 2 0.034842443125853606 1104 3 -0.009496494931923394 1104 4 0.006815996052971229 1104 5 0.03364410349187352 1104 6 -0.06105981158631124 1104 7 -0.06779706009599096 1104 8 0.019350095698641212 1104 9 0.06224094948199621 1104 10-11 0.019182821180720566 1104 12-13 0.09856092145507489 1104 14-15 0.0539304192649368 1104 16-17 0.06435544716961772 1104 18-19 0.08892512342282544 1104 20-21 0.011723820478749758 1104 22-23 -0.008752589494989138 1104 24-25 -0.03428629235289549 1104 26-27 -0.10113239522523543 1104 28-29 0.010042193127631549 1104 30-31 -0.07737392023219769 1104 32-33 0.05503514213278038 1104 34-35 0.015195118083560999 1104 36-37 0.03842171674688899 1104 38-39 0.12229508924008314 1104 40-41 0.21219288886373278 1104 42-43 0.0278453974126478 1104 44-45 0.08275557559593949 1104 46-47 0.21566966187822345 1104 48-49 0.11012025940831194 1104 50-51 0.022341664204859057 1104 52-53 -0.023940488833140705 1104 54-55 0.12663131373334835 1104 56-57 0.14408881403204532 1104 58-59 0.17776416167308895 1104 60-61 0.24321546608542377 1104 62-63 0.29877496188488095 1104 64-65 0.21516726266217034 1104 66-67 0.24913391141133445 1104 68-69 0.17562173076611742 1104 70-71 0.1124122896548343 1104 72-73 0.09934596787789474 1104 74-75 0.17547973670490435 1104 76-77 0.09640622282093148 1104 78-79 0.17925693066117887 1104 80-81 0.31566479762654964 1104 82-83 0.3336746765443692 1104 84-85 0.20937599654954298 1104 86-87 0.2223405328435284 1104 88-89 0.3425404799947316 1104 90-91 0.47873433550805444 1104 92-93 0.41330172786087616 1104 94-95 0.49066849141609126 1104 96-97 0.3950717358470719 1104 98-99 0.4520328676032399 1104 100-101 0.9200197531541754 1105 1 -0.0055198084297387595 1105 2 -0.042453556926048464 1105 3 -0.009739775409293827 1105 4 0.036705435502312866 1105 5 0.07747050225035679 1105 6 0.04291919122322696 1105 7 -0.019692400371233987 1105 8 0.03136977315043055 1105 9 -0.05250194012473486 1105 10-11 1.9651763990680138E-4 1105 12-13 0.020605167918127165 1105 14-15 0.05575606547580492 1105 16-17 0.13550826762445922 1105 18-19 0.038814094846863156 1105 20-21 0.01887499218703681 1105 22-23 -0.09865086581805116 1105 24-25 0.035132401689701 1105 26-27 -0.048912704149735475 1105 28-29 -0.03357718552939559 1105 30-31 -0.05419519897926506 1105 32-33 -0.07754889505345375 1105 34-35 -0.014629909963197463 1105 36-37 -0.10410461431011697 1105 38-39 0.02669130648352791 1105 40-41 -0.017055170568994527 1105 42-43 -0.13513155396322674 1105 44-45 -0.07887070573916333 1105 46-47 -0.0895205996737829 1105 48-49 -0.12256570071315309 1105 50-51 -0.029007430305206583 1105 52-53 0.08730449090044345 1105 54-55 0.09314943256821806 1105 56-57 0.24721436575741507 1105 58-59 0.1432971104224663 1105 60-61 0.08459752162473677 1105 62-63 0.02447506517481912 1105 64-65 0.0812842235746487 1105 66-67 0.19929512527433957 1105 68-69 0.06704727558696533 1105 70-71 0.22361498492448817 1105 72-73 0.2615641158600752 1105 74-75 0.3131900410928061 1105 76-77 0.11180819399520914 1105 78-79 0.18553866428249677 1105 80-81 0.1498798103026182 1105 82-83 0.3068767527231344 1105 84-85 0.18857250369063294 1105 86-87 0.09870956476795101 1105 88-89 0.3294199004589302 1105 90-91 0.5915914538716507 1105 92-93 0.6181408777601121 1105 94-95 0.7271146409348859 1105 96-97 0.5899807558994077 1105 98-99 0.6506070818188832 1105 100-101 1.0653654732481677 1106 1 -0.058805060903381445 1106 2 -0.08961414390495293 1106 3 -0.11929549208747403 1106 4 -0.049819317830397836 1106 5 0.008112315917294666 1106 6 -0.018804128732561765 1106 7 -0.10409889645154635 1106 8 -0.02620340783899877 1106 9 -0.038229639183214204 1106 10-11 -0.0168390007584307 1106 12-13 -0.008459058235878558 1106 14-15 0.012419195620203993 1106 16-17 -0.022140914698539405 1106 18-19 -0.06302666385027322 1106 20-21 0.02252755778046378 1106 22-23 0.12928389734273793 1106 24-25 0.05361299655665874 1106 26-27 0.10681793579018262 1106 28-29 0.09586375679573678 1106 30-31 0.09081596243125745 1106 32-33 0.015611464789785146 1106 34-35 -0.009507817682589348 1106 36-37 -0.1268424035396336 1106 38-39 0.03686682477674452 1106 40-41 0.18694294287956126 1106 42-43 0.22176600122057266 1106 44-45 0.22691115398102113 1106 46-47 0.3108405323393484 1106 48-49 0.13545174303249752 1106 50-51 0.0657256270072466 1106 52-53 0.15659161164499125 1106 54-55 0.13029251364765315 1106 56-57 0.08734398390669185 1106 58-59 0.06103606389370242 1106 60-61 0.04769323006296844 1106 62-63 -0.06909135110180387 1106 64-65 -0.011136917044048289 1106 66-67 0.044786310742040314 1106 68-69 0.030992000726513425 1106 70-71 0.04688217851240495 1106 72-73 0.2382231831550783 1106 74-75 0.1844001144000451 1106 76-77 0.039829606902500814 1106 78-79 0.16104731451846277 1106 80-81 0.17837345518810466 1106 82-83 0.25518362078952705 1106 84-85 0.23384528243969172 1106 86-87 0.09213839715119221 1106 88-89 0.3320003125433715 1106 90-91 0.47673015402034125 1106 92-93 0.3593973539260631 1106 94-95 0.4195432308724989 1106 96-97 0.5706982167145043 1106 98-99 0.6503144789697224 1106 100-101 0.9007464933432772 1107 1 0.01742145619352442 1107 2 -0.01691890224753223 1107 3 -0.1267194997314931 1107 4 -0.07071151885065063 1107 5 -0.19529983594483724 1107 6 -0.05335782392078414 1107 7 -0.08571646262170418 1107 8 -0.03161268690561769 1107 9 -0.024062105544167878 1107 10-11 0.04597747035488453 1107 12-13 0.07604796188311269 1107 14-15 0.17265926577425006 1107 16-17 0.08245445802624829 1107 18-19 -0.08756407776855468 1107 20-21 -0.0035114732639627277 1107 22-23 0.01696827958168967 1107 24-25 -0.0350311319710741 1107 26-27 -0.04511098609430064 1107 28-29 0.01572250577815737 1107 30-31 0.017650041203687294 1107 32-33 0.05128567594967848 1107 34-35 0.07128641283882331 1107 36-37 0.08169457663053237 1107 38-39 -0.057465533147173176 1107 40-41 0.08344922751262374 1107 42-43 -0.0035582883597271575 1107 44-45 0.08304900416190719 1107 46-47 -0.0044409167565362395 1107 48-49 0.046683461963716866 1107 50-51 -0.011256020418393575 1107 52-53 0.12127728569899432 1107 54-55 0.11055147384655584 1107 56-57 0.10487227968519619 1107 58-59 0.2639374616312935 1107 60-61 0.052194152563494356 1107 62-63 0.054192122151967226 1107 64-65 0.12893386561986375 1107 66-67 0.006487609157602492 1107 68-69 -0.07942596901798282 1107 70-71 -0.008140184660497596 1107 72-73 -0.017671705217644273 1107 74-75 0.04143296296223298 1107 76-77 -0.2733365343422314 1107 78-79 -0.015254794188873433 1107 80-81 0.2783303946865274 1107 82-83 0.32527087330950977 1107 84-85 0.03199603819863839 1107 86-87 0.08451401673523407 1107 88-89 0.2995187075496091 1107 90-91 0.48154774407808176 1107 92-93 0.640485778823205 1107 94-95 0.5705873694581669 1107 96-97 0.5328497645352357 1107 98-99 0.39891501266849616 1107 100-101 0.8382836212392526 1108 1 -0.05308639916968616 1108 2 -0.07438689157331169 1108 3 -0.018822826481041943 1108 4 -0.0028869722007272003 1108 5 0.01547314138443312 1108 6 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quality scores pass #Quality Count 2 2.0 3 1.0 4 16.0 5 37.0 6 92.0 7 247.0 8 574.0 9 1148.0 10 1888.0 11 2967.0 12 4196.0 13 5714.0 14 7424.0 15 9875.0 16 13099.0 17 19040.0 18 19329.0 19 13198.0 20 12035.0 21 12672.0 22 13658.0 23 14849.0 24 17084.0 25 20593.0 26 24479.0 27 29920.0 28 36434.0 29 44031.0 30 53790.0 31 64161.0 32 79411.0 33 101546.0 34 136653.0 35 195121.0 36 307767.0 37 483288.0 38 366582.0 39 26862.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.516667597245046 20.408240826804036 16.550297448823272 21.52479412712764 2 15.52694829335498 21.619949312617216 38.97862540266934 23.87447699135847 3 19.167364167301077 25.485855341406115 30.91748088474392 24.429299606548888 4 13.34032469647623 18.551133456056057 37.52586126724065 30.582680580227063 5 13.747666809672424 37.66818364930128 33.91161870365749 14.672530837368805 6 29.983227271176567 37.091377957484475 17.80161820147183 15.12377656986713 7 27.098448767935814 33.87198608457026 20.90450293324136 18.125062214252566 8 26.11975139535177 34.374046340213006 19.76602300326715 19.740179261168073 9 25.81831896038056 17.63473212003273 20.921514003990126 35.625434915596585 10-11 24.7460373318229 27.176494065052392 26.92132800382095 21.15614059930376 12-13 26.184430851165747 24.59426960584321 27.401143013099926 21.820156529891115 14-15 23.06953724788264 25.7563666825953 26.59412712451557 24.579968945006488 16-17 21.744352525155517 28.962918149615874 27.12346526294439 22.169264062284217 18-19 21.57919751675754 27.634624632497783 29.413216199960463 21.372961650784212 20-21 22.941298253140623 26.34650336038748 29.250582886208555 21.461615500263346 22-23 22.359673854778734 26.422585841648427 29.221958488314005 21.995781815258837 24-25 22.380003018997723 26.616320439969847 29.33587190850661 21.667804632525822 26-27 22.185871184134093 26.52028266417669 29.53191982551502 21.761926326174198 28-29 21.88946262307907 26.95233582096876 29.418006405322412 21.74019515062976 30-31 22.08632838002732 26.609894554728214 29.25728917371528 22.04648789152919 32-33 21.947342323964627 26.726448429583748 29.161905669874 22.164303576577623 34-35 22.512091427973225 26.612778474384026 29.054080894912882 21.821049202729867 36-37 22.50368169239606 26.333973783452457 29.305850841332727 21.856493682818755 38-39 22.2510967382495 26.61206267182544 29.290039008640235 21.84680158128483 40-41 22.353243303366963 26.588703632043636 29.22625356820387 21.83179949638553 42-43 22.10822757035923 26.763917039643065 28.781050664814757 22.346804725182952 44-45 22.36981538042372 26.788683968924822 28.606091705286318 22.23540894536514 46-47 22.584414774159825 26.9152876480324 28.200234931078082 22.300062646729693 48-49 22.60110324400096 26.947483632082825 28.17515628355338 22.276256840362834 50-51 22.374889094466397 27.144697459518458 28.211283307586815 22.269130138428334 52-53 22.5914263268752 27.34424472014218 27.73580311648424 22.328525836498375 54-55 22.36079546383909 27.04926153726803 28.01206944816367 22.577873550729212 56-57 22.505389565203572 27.449816172948378 27.698649816359882 22.346144445488164 58-59 22.71948304917613 27.057012456432396 27.838799165147037 22.384705329244436 60-61 22.52392877530891 26.919098178256146 28.327379715857404 22.22959333057754 62-63 22.523568978723542 26.972618251476906 28.209028672533616 22.29478409726594 64-65 22.30998837729352 27.18886126918833 28.0796550294727 22.42149532404545 66-67 22.312982618152212 27.12833349145068 27.83719770572508 22.721486184672028 68-69 22.209479345206173 27.115502574473975 28.089514816515422 22.585503263804426 70-71 22.40956209110924 27.007341398637152 27.843711254832847 22.73938525542076 72-73 22.357454003513443 26.837043756306127 28.01206944816367 22.79343279201676 74-75 22.230291576295354 27.416144534282218 27.581278101564504 22.772285787857925 76-77 22.356369584783174 27.029626459968732 27.731001017471545 22.88300293777655 78-79 22.324854739807485 27.121397002777385 27.796372619440962 22.757375637974167 80-81 22.333853479535072 27.221218226334166 27.63168040871434 22.81324788541642 82-83 22.29497103210933 27.19507071511457 27.79351924938183 22.71643900339427 84-85 22.213140304414043 27.14896790936277 27.655701536090344 22.98219025013284 86-87 22.223641873471635 27.086711830297073 27.77277779727848 22.916868498952812 88-89 22.340572589429108 26.970521879354287 27.572063013141157 23.116842518075455 90-91 22.128073310952352 27.02473888271884 27.7794463087445 23.067741497584304 92-93 22.304785111387464 27.128778946276327 27.696009361696955 22.870426580639254 94-95 22.091001751112145 27.021712014722986 27.65539776696983 23.231888467195038 96-97 21.89337594849541 26.937117789033916 27.75430823071914 23.415198031751537 98-99 21.885350056524423 27.00397657145608 27.444091293369468 23.66658207865003 100-101 22.13147046356613 26.34444833373135 28.170102454410056 23.353978748292466 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 327.0 1 248.0 2 208.5 3 255.5 4 226.5 5 222.0 6 442.5 7 1003.0 8 1956.5 9 2632.5 10 2411.0 11 2013.0 12 2019.5 13 2217.5 14 2453.0 15 2618.5 16 2689.5 17 2854.5 18 3083.5 19 3288.0 20 3624.0 21 4208.0 22 5077.0 23 6276.5 24 7654.0 25 9349.0 26 12163.5 27 15700.0 28 18855.5 29 21999.5 30 25372.0 31 29498.0 32 33736.5 33 37659.0 34 42298.5 35 46472.0 36 50991.5 37 55672.5 38 58348.5 39 59564.0 40 61198.5 41 64300.5 42 67351.5 43 70027.0 44 73322.5 45 75730.0 46 76789.5 47 82633.0 48 98500.5 49 98720.0 50 83778.5 51 76941.0 52 73211.5 53 71817.5 54 69740.0 55 64184.0 56 59059.5 57 53690.0 58 48105.5 59 43056.0 60 36411.0 61 29089.5 62 22515.5 63 16913.0 64 12421.0 65 9162.0 66 6708.5 67 5170.0 68 3790.0 69 2639.0 70 1878.5 71 1213.5 72 804.0 73 536.0 74 358.0 75 239.0 76 132.5 77 53.0 78 26.5 79 16.5 80 7.0 81 5.0 82 2.5 83 3.0 84 2.0 85 1.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3968626725233353 2 0.0 3 0.0 4 0.0 5 4.206033976342461E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014253781808716119 16-17 0.002032916421898856 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002056283277322981 36-37 0.006636186940451439 38-39 0.01201056368800014 40-41 0.002383419253260728 42-43 6.542719518754939E-4 44-45 2.1030169881712304E-4 46-47 3.037691205136222E-4 48-49 1.168342771206239E-4 50-51 5.3743767475487E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.336685542412478E-4 60-61 0.002219851265291854 62-63 0.0 64-65 2.804022650894974E-4 66-67 0.0010281416386614904 68-69 0.001191709626630364 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12255915669953449 78-79 9.346742169649912E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14524837331635965 88-89 0.4364227587563786 90-91 0.335010606215677 92-93 0.0 94-95 0.0 96-97 5.841713856031196E-4 98-99 0.0 100-101 9.346742169649912E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2139783.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.969022885328314 #Duplication Level Percentage of deduplicated Percentage of total 1 77.42233978674132 44.88097386933272 2 13.308564170966712 15.429689219952595 3 4.086865907675027 7.107348699938451 4 1.7215671770777405 3.9919026834659843 5 0.9537054719547318 2.7642687164803346 6 0.5525329983727488 1.921787881654137 7 0.3736914900194147 1.5163771375891524 8 0.2575779740517151 1.1945234778048304 9 0.19328911559573042 1.008430304890839 >10 1.021413706520512 10.737910911609232 >50 0.07207437517749037 2.892499787926294 >100 0.03315328922392005 3.5020177676371222 >500 0.0017734951426247455 0.7202619640900165 >1k 0.0012092012336077467 1.3029484255341217 >5k 2.4184024672154933E-4 1.0290591520940713 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9007 0.42093053361018384 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7961 0.3720470720629148 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5053 0.23614544091620504 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4052 0.18936499635710724 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2666 0.12459207312143335 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2564 0.11982523461491189 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2475 0.11566593434941766 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2191 0.1023935604685148 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.673371084824957E-5 0.0 4 0.0 0.0 0.0 4.673371084824957E-5 0.0 5 0.0 0.0 0.0 4.673371084824957E-5 0.0 6 0.0 0.0 0.0 4.673371084824957E-5 0.0 7 0.0 0.0 0.0 9.346742169649914E-5 0.0 8 0.0 0.0 0.0 9.346742169649914E-5 0.0 9 0.0 0.0 0.0 1.402011325447487E-4 0.0 10-11 0.0 0.0 0.0 1.402011325447487E-4 0.0 12-13 0.0 0.0 0.0 2.3366855424124784E-4 4.673371084824957E-5 14-15 0.0 0.0 0.0 2.804022650894974E-4 4.673371084824957E-5 16-17 0.0 0.0 0.0 2.804022650894974E-4 4.673371084824957E-5 18-19 0.0 0.0 0.0 2.804022650894974E-4 4.673371084824957E-5 20-21 0.0 0.0 0.0 4.673371084824956E-4 7.010056627237435E-5 22-23 0.0 0.0 0.0 5.841713856031196E-4 9.346742169649914E-5 24-25 0.0 0.0 0.0 6.776388072996187E-4 9.346742169649914E-5 26-27 0.0 0.0 0.0 0.0010047747832373655 9.346742169649914E-5 28-29 0.0 0.0 0.0 0.0022198512652918546 9.346742169649914E-5 30-31 0.0 0.0 0.0 0.00479020536194558 9.346742169649914E-5 32-33 0.0 0.0 0.0 0.009463576446770539 9.346742169649914E-5 34-35 0.0 0.0 0.0 0.016940970182490467 9.346742169649914E-5 36-37 0.0 0.0 0.0 0.029395504123548976 9.346742169649914E-5 38-39 0.0 0.0 0.0 0.049911603185930534 9.346742169649914E-5 40-41 0.0 0.0 0.0 0.07404956483905142 9.346742169649914E-5 42-43 2.3366855424124784E-5 0.0 0.0 0.10061767945628132 9.346742169649914E-5 44-45 4.673371084824957E-5 0.0 0.0 0.13286393994157353 9.346742169649914E-5 46-47 4.673371084824957E-5 4.673371084824957E-5 0.0 0.17438684203024324 9.346742169649914E-5 48-49 4.673371084824957E-5 4.673371084824957E-5 0.0 0.2216579905532477 9.346742169649914E-5 50-51 4.673371084824957E-5 4.673371084824957E-5 0.0 0.2697703458715206 9.346742169649914E-5 52-53 4.673371084824957E-5 4.673371084824957E-5 0.0 0.32199526774443954 1.8693484339299827E-4 54-55 4.673371084824957E-5 4.673371084824957E-5 0.0 0.3843380380160044 1.8693484339299827E-4 56-57 4.673371084824957E-5 4.673371084824957E-5 0.0 0.4630376070844567 1.8693484339299827E-4 58-59 4.673371084824957E-5 4.673371084824957E-5 0.0 0.5505932143586523 1.8693484339299827E-4 60-61 4.673371084824957E-5 4.673371084824957E-5 0.0 0.6488274745616729 1.8693484339299827E-4 62-63 4.673371084824957E-5 4.673371084824957E-5 0.0 0.74942178716253 1.8693484339299827E-4 64-65 4.673371084824957E-5 4.673371084824957E-5 0.0 0.8608583206801812 1.8693484339299827E-4 66-67 4.673371084824957E-5 4.673371084824957E-5 0.0 0.9921800481637624 2.1030169881712307E-4 68-69 4.673371084824957E-5 4.673371084824957E-5 0.0 1.1440412415651493 2.3366855424124784E-4 70-71 4.673371084824957E-5 4.673371084824957E-5 0.0 1.3127265708719063 2.3366855424124784E-4 72-73 4.673371084824957E-5 4.673371084824957E-5 0.0 1.4887023590709898 2.570354096653726E-4 74-75 4.673371084824957E-5 4.673371084824957E-5 0.0 1.680661076380175 2.804022650894974E-4 76-77 4.673371084824957E-5 4.673371084824957E-5 0.0 1.8880886519801308 2.804022650894974E-4 78-79 4.673371084824957E-5 4.673371084824957E-5 0.0 2.133650935632258 2.804022650894974E-4 80-81 4.673371084824957E-5 4.673371084824957E-5 0.0 2.410968775805771 2.804022650894974E-4 82-83 4.673371084824957E-5 4.673371084824957E-5 0.0 2.690740135798817 3.7386968678599654E-4 84-85 4.673371084824957E-5 4.673371084824957E-5 0.0 2.990560257745762 3.7386968678599654E-4 86-87 4.673371084824957E-5 4.673371084824957E-5 0.0 3.311340449008147 3.9723654221012134E-4 88-89 4.673371084824957E-5 4.673371084824957E-5 0.0 3.6568895070201046 4.206033976342461E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 7590 0.0 22.602064 12-13 GTACTTT 7775 0.0 21.972713 14-15 GTATCAA 21060 0.0 20.044529 1 TTTGCGT 410 0.0 19.675987 3 AGTACTT 7975 0.0 19.279507 12-13 GTACATG 10065 0.0 19.12441 1 TACATGG 10030 0.0 18.924765 2 GGTATCA 16750 0.0 18.347013 1 ACTTTTT 9365 0.0 18.343292 16-17 TATCAAC 23765 0.0 17.69161 2 ACATGGG 10040 0.0 17.677032 3 ATCAACG 23765 0.0 17.671642 3 TCAACGC 23865 0.0 17.657246 4 CAACGCA 23905 0.0 17.60785 5 AACGCAG 24190 0.0 17.400398 6 CTTTGCG 485 1.8189894E-12 16.633308 2 GTGTAGC 1340 0.0 16.355865 1 AGAGTAC 17690 0.0 16.31013 10-11 TACTTTT 9300 0.0 16.252048 14-15 CATGGGG 4990 0.0 16.166641 4 >>END_MODULE