##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938915_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 265214 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.662608308761982 33.0 31.0 34.0 30.0 34.0 2 31.627055886944127 33.0 31.0 34.0 30.0 34.0 3 31.709280053089202 33.0 31.0 34.0 30.0 34.0 4 35.108142858220155 37.0 35.0 37.0 33.0 37.0 5 35.19872254104233 37.0 35.0 37.0 33.0 37.0 6 35.41963471008318 37.0 35.0 37.0 33.0 37.0 7 35.40131365614183 37.0 35.0 37.0 33.0 37.0 8 35.43961103109187 37.0 35.0 37.0 33.0 37.0 9 36.904088773594154 39.0 37.0 39.0 33.0 39.0 10-11 36.78812958591929 39.0 37.0 39.0 32.5 39.0 12-13 36.78388584313045 39.0 37.0 39.0 33.0 39.0 14-15 37.70008747652839 40.0 37.5 41.0 32.0 41.0 16-17 37.724884432948485 40.0 37.5 41.0 32.5 41.0 18-19 37.733560445527004 40.0 37.0 41.0 32.5 41.0 20-21 37.835197236948275 40.0 38.0 41.0 33.0 41.0 22-23 37.822669617742655 40.0 38.0 41.0 33.0 41.0 24-25 37.596578611988804 40.0 37.5 41.0 32.5 41.0 26-27 37.37073646187607 39.5 37.0 41.0 31.5 41.0 28-29 37.382289396487366 40.0 37.0 41.0 32.0 41.0 30-31 37.421844246532984 40.0 37.0 41.0 32.0 41.0 32-33 37.130551177539644 40.0 36.0 41.0 31.0 41.0 34-35 36.66203330140943 39.0 35.0 41.0 30.0 41.0 36-37 36.29311612509143 39.0 35.5 40.5 30.0 41.0 38-39 36.044518765977664 39.0 35.0 40.0 30.0 41.0 40-41 36.2476132481694 39.0 35.0 40.5 30.0 41.0 42-43 35.9763964194952 39.0 35.0 40.5 30.0 41.0 44-45 35.94185073186182 39.0 35.0 41.0 30.0 41.0 46-47 35.68437186573861 39.0 35.0 41.0 27.5 41.0 48-49 35.30536660960583 39.0 35.0 40.5 26.5 41.0 50-51 34.10422903768278 38.0 34.0 39.5 24.0 40.5 52-53 34.45201799301696 38.0 34.0 39.5 25.0 40.5 54-55 35.11566508555355 39.0 35.0 40.0 26.0 41.0 56-57 34.850980717458356 38.5 34.5 40.0 24.5 41.0 58-59 34.7140988032306 39.0 34.0 40.0 23.5 41.0 60-61 33.998144894311764 38.0 33.0 40.0 22.0 41.0 62-63 33.78968870421622 38.0 33.0 40.0 22.0 41.0 64-65 33.39121803524701 37.0 33.0 39.5 21.5 40.5 66-67 32.542999238350916 36.0 32.0 39.0 18.0 40.0 68-69 32.027775683033326 36.0 31.5 38.5 16.0 39.0 70-71 31.138003649882734 35.0 30.5 37.0 10.5 39.0 72-73 30.55290821751491 34.5 29.5 37.0 7.5 37.5 74-75 30.17509256675741 34.0 30.0 36.5 4.0 37.0 76-77 29.77163535861606 34.0 30.0 35.5 2.0 36.5 78-79 29.242915155308545 34.0 29.0 35.0 2.0 36.0 80-81 28.621892132391203 34.0 29.0 35.0 2.0 36.0 82-83 28.042524150308807 33.5 27.5 35.0 2.0 35.5 84-85 27.512878279427177 33.0 26.0 35.0 2.0 35.0 86-87 27.389166861477904 34.0 26.0 35.0 2.0 35.0 88-89 26.664893256012128 33.0 25.0 35.0 2.0 35.0 90-91 26.0218691320971 33.0 24.0 35.0 2.0 35.0 92-93 25.463152397686397 32.0 20.0 35.0 2.0 35.0 94-95 24.74088283423952 32.0 17.5 34.0 2.0 35.0 96-97 23.868264872895097 31.0 4.0 34.0 2.0 35.0 98-99 23.12148491406939 31.0 2.0 34.0 2.0 35.0 100-101 19.761066534949137 25.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09876540936499723 1101 2 -0.010220520192724791 1101 3 0.10349329445137911 1101 4 0.2235879045296869 1101 5 -0.5340891501169338 1101 6 0.08975782343512861 1101 7 0.08493077098692936 1101 8 0.13161706797271933 1101 9 0.41177231834065964 1101 10-11 0.15055633423751402 1101 12-13 -0.15156406924497645 1101 14-15 0.2903645411631004 1101 16-17 0.15799414247216959 1101 18-19 0.31742447662053763 1101 20-21 0.20241718671837816 1101 22-23 0.5522994376373731 1101 24-25 0.39779813910978845 1101 26-27 0.2956272784663625 1101 28-29 0.22716911277228036 1101 30-31 0.395100187376606 1101 32-33 0.30258489996121085 1101 34-35 0.5631620389062064 1101 36-37 0.39854602409164386 1101 38-39 0.6265447338458401 1101 40-41 0.5113122383437769 1101 42-43 0.5814162053477503 1101 44-45 0.8404342030636585 1101 46-47 0.8683823074396457 1101 48-49 1.1342671603817038 1101 50-51 0.9841128073954835 1101 52-53 0.7137056451515775 1101 54-55 0.8502439224396738 1101 56-57 1.0031105327281722 1101 58-59 1.2874546838962004 1101 60-61 1.7479491571659693 1101 62-63 1.0251081111729263 1101 64-65 0.6707138346261843 1101 66-67 0.6763613551112755 1101 68-69 0.6586460176469799 1101 70-71 1.0616481292753726 1101 72-73 0.9226781639537514 1101 74-75 0.8282288498918966 1101 76-77 0.3805856176516862 1101 78-79 0.6859344426408391 1101 80-81 0.7180242029398407 1101 82-83 0.6213148301933522 1101 84-85 0.26564833325928916 1101 86-87 0.5529824897620195 1101 88-89 0.17446156510780852 1101 90-91 -0.13250578955525683 1101 92-93 -0.07321058587765705 1101 94-95 0.3274412652214238 1101 96-97 0.6697737384280451 1101 98-99 0.2947923316070984 1101 100-101 0.6654254827639825 1102 1 0.1891590563635468 1102 2 0.33559110989864394 1102 3 0.21246776289984837 1102 4 -0.19039894544042824 1102 5 0.29422610904111934 1102 6 0.516484832230276 1102 7 0.42787330364852494 1102 8 0.23454593826560455 1102 9 0.5074259772937779 1102 10-11 0.5760537298484749 1102 12-13 0.40243644309385473 1102 14-15 0.4811509812621466 1102 16-17 0.7912620102887544 1102 18-19 0.6290460290071778 1102 20-21 0.6248149324275474 1102 22-23 0.6794960903570342 1102 24-25 1.0392975840760599 1102 26-27 1.1833909194876284 1102 28-29 1.1028330185019328 1102 30-31 1.01964121717765 1102 32-33 1.1219513076121288 1102 34-35 0.8728110868098824 1102 36-37 0.552153743329967 1102 38-39 0.9377222592493055 1102 40-41 1.2617263788953963 1102 42-43 1.364261393632269 1102 44-45 1.427463491766595 1102 46-47 1.2319977117603784 1102 48-49 0.9782770655990234 1102 50-51 0.9323836638552265 1102 52-53 1.0824871326419654 1102 54-55 1.11619046842209 1102 56-57 1.4159958542207818 1102 58-59 1.1753037147219203 1102 60-61 0.9650868909205528 1102 62-63 1.3733448117108864 1102 64-65 1.2149115974133053 1102 66-67 1.5195856741275904 1102 68-69 1.3299123484470883 1102 70-71 0.7137273710139098 1102 72-73 0.4621709485152721 1102 74-75 0.5005454752868239 1102 76-77 0.5737885892168784 1102 78-79 0.5407120022002267 1102 80-81 0.6525985347646177 1102 82-83 0.739665099171237 1102 84-85 0.46962749814699833 1102 86-87 0.8927058267955807 1102 88-89 1.0032037732881527 1102 90-91 0.9931538466841765 1102 92-93 1.453670124565516 1102 94-95 1.520985796004446 1102 96-97 1.4925642772377081 1102 98-99 1.9483658949236364 1102 100-101 2.4423360796387144 1103 1 -0.27188731453448156 1103 2 -0.3125986615319043 1103 3 -0.2993065031536126 1103 4 -0.36720312262497856 1103 5 -0.3399417390864059 1103 6 -0.4329947486680936 1103 7 -0.3676244659838943 1103 8 -0.3650771469033174 1103 9 -0.11330779634984367 1103 10-11 -0.054621604706660776 1103 12-13 -0.060848940218157566 1103 14-15 -0.1681107497797285 1103 16-17 -0.060288873213437455 1103 18-19 0.049816110960222204 1103 20-21 0.0070722718929445705 1103 22-23 -0.07290717393287593 1103 24-25 0.0968030303224694 1103 26-27 0.3476427954169523 1103 28-29 0.35688744638671466 1103 30-31 0.06093759672051391 1103 32-33 0.019172857458251258 1103 34-35 0.1840424571892143 1103 36-37 0.36592669536841527 1103 38-39 0.530204710233086 1103 40-41 0.3105869895665805 1103 42-43 -0.08676561283406414 1103 44-45 0.11481436835291703 1103 46-47 -0.07706380488958331 1103 48-49 0.05930510944563139 1103 50-51 -0.13027410439402587 1103 52-53 0.47432294992471924 1103 54-55 0.33797372510454693 1103 56-57 -0.23412171554303285 1103 58-59 0.03166112680393951 1103 60-61 0.5793456827183334 1103 62-63 0.4429357380914638 1103 64-65 0.4652660356718883 1103 66-67 0.5844824547908232 1103 68-69 0.5913581107486863 1103 70-71 0.7028608126958495 1103 72-73 0.31717976624755906 1103 74-75 0.29348437358897783 1103 76-77 -0.009547625728313136 1103 78-79 0.10699195655549687 1103 80-81 0.3138160734070361 1103 82-83 0.6756241572299508 1103 84-85 0.4138942431935071 1103 86-87 0.4903665342889205 1103 88-89 0.4824561679076069 1103 90-91 0.07830547710016944 1103 92-93 -0.2520856069604669 1103 94-95 -0.4116968693712586 1103 96-97 -0.4125672971597467 1103 98-99 0.0906685333274524 1103 100-101 0.09245364940253964 1104 1 0.257038305300231 1104 2 0.3898806773110728 1104 3 0.33705713321002406 1104 4 0.24849935671386447 1104 5 0.20964503777309318 1104 6 0.21989781331537728 1104 7 0.17783007947065244 1104 8 0.2258319440057761 1104 9 0.24619633588155665 1104 10-11 0.4061221969462352 1104 12-13 0.40196097713721457 1104 14-15 0.39180660559217273 1104 16-17 0.47276897802622386 1104 18-19 0.5043615655056826 1104 20-21 0.2983945859425319 1104 22-23 0.33618373515803057 1104 24-25 0.5575468319753654 1104 26-27 0.46334527475578824 1104 28-29 0.4023419918412614 1104 30-31 0.4720946215138966 1104 32-33 0.6348753367971014 1104 34-35 0.39685237454458644 1104 36-37 0.9651002490874276 1104 38-39 0.7740063631256575 1104 40-41 0.7795952540293953 1104 42-43 0.6115814946039464 1104 44-45 0.970186269179365 1104 46-47 0.7431841289947201 1104 48-49 0.3712885805200159 1104 50-51 0.17964325097787537 1104 52-53 0.1788684044701796 1104 54-55 0.28632083254255747 1104 56-57 0.18943200346522104 1104 58-59 0.06058674663864849 1104 60-61 0.3086845256935433 1104 62-63 0.40574565544683594 1104 64-65 0.41981149021734154 1104 66-67 0.2993584878486715 1104 68-69 0.39920935041811134 1104 70-71 0.4549269283983257 1104 72-73 0.5341219150078906 1104 74-75 0.07447610912617009 1104 76-77 0.47185733294937293 1104 78-79 0.28881013837367675 1104 80-81 0.8138160734070361 1104 82-83 0.7706654795440038 1104 84-85 0.38910085476375755 1104 86-87 0.3395400880079329 1104 88-89 0.45972889518033355 1104 90-91 0.5431815101580177 1104 92-93 0.7747738971717624 1104 94-95 0.8093775107940289 1104 96-97 0.2837136945757912 1104 98-99 0.32413960770762174 1104 100-101 0.6131148064273297 1105 1 0.560585137256151 1105 2 0.560782881167821 1105 3 0.5835103012541012 1105 4 0.6723974283392096 1105 5 0.48801969342047613 1105 6 0.7334653064283216 1105 7 0.5118865532998527 1105 8 0.6649504013060508 1105 9 0.9849015700413375 1105 10-11 0.6494934090674462 1105 12-13 0.7661655225917556 1105 14-15 1.142788010550852 1105 16-17 0.8682407411116344 1105 18-19 0.9065826398858476 1105 20-21 0.8789903159700785 1105 22-23 0.7897994376373703 1105 24-25 0.9051535812866618 1105 26-27 1.00804224445276 1105 28-29 0.6194735345409867 1105 30-31 0.9112991669684405 1105 32-33 0.8374063285326372 1105 34-35 1.0475582974096014 1105 36-37 1.1902552077651123 1105 38-39 1.0292233052744137 1105 40-41 1.228148973037655 1105 42-43 1.0501662053477503 1105 44-45 1.2466842030636585 1105 46-47 0.9322853686641395 1105 48-49 0.9207892692252457 1105 50-51 0.8113236917492301 1105 52-53 0.9218263934508926 1105 54-55 0.9775398408070259 1105 56-57 1.4648452266057248 1105 58-59 1.3402182893383667 1105 60-61 1.1632127626081399 1105 62-63 1.226596206411024 1105 64-65 0.876836283605769 1105 66-67 1.4358426476282844 1105 68-69 1.91778717410957 1105 70-71 1.491325000023675 1105 72-73 1.3620489122530692 1105 74-75 1.3830332716606044 1105 76-77 1.5180005836380808 1105 78-79 1.5664616535251916 1105 80-81 1.8845633185860997 1105 82-83 2.0924457825743055 1105 84-85 2.1758949318987426 1105 86-87 1.9144195645919524 1105 88-89 2.2566469392574717 1105 90-91 2.2287187002406625 1105 92-93 2.1602077814692855 1105 94-95 2.5533341223642765 1105 96-97 2.7057006091763434 1105 98-99 2.350362059498252 1105 100-101 2.6330700405871106 1106 1 -0.18230701960659346 1106 2 0.11887111646193915 1106 3 0.14110834046978837 1106 4 0.28073076167254385 1106 5 -0.007078345795207497 1106 6 -0.012122928865792915 1106 7 -0.1697310937589691 1106 8 -0.08014763790963286 1106 9 -0.23568666525277848 1106 10-11 -0.1670507085796089 1106 12-13 -0.07905506564353715 1106 14-15 -0.05145218552758024 1106 16-17 -0.08286220006484513 1106 18-19 0.17900911047407675 1106 20-21 0.01318149244067257 1106 22-23 0.11112296704913405 1106 24-25 0.0015996597180318872 1106 26-27 0.154610871903742 1106 28-29 -0.23089411251783787 1106 30-31 0.08617661794883702 1106 32-33 0.22527397559145612 1106 34-35 0.1513573170174496 1106 36-37 0.016113050902369253 1106 38-39 0.21562036409793706 1106 40-41 0.5149136789200099 1106 42-43 0.583376989661474 1106 44-45 0.21886557561268205 1106 46-47 0.15397654513473213 1106 48-49 0.6086569162840618 1106 50-51 0.7368138878276582 1106 52-53 0.5278312954116728 1106 54-55 0.5380790564932951 1106 56-57 0.25553150111552014 1106 58-59 0.26656632855406315 1106 60-61 0.4878451155493124 1106 62-63 0.23995404954828103 1106 64-65 0.006248048311654486 1106 66-67 -0.11991715629328326 1106 68-69 -0.025227531772785028 1106 70-71 -0.15695931370181526 1106 72-73 0.1269998926452267 1106 74-75 -0.016108885202140044 1106 76-77 -0.1800386320481948 1106 78-79 0.1794518496036268 1106 80-81 0.34963684799786066 1106 82-83 -0.1419904919354984 1106 84-85 -0.2928550681012574 1106 86-87 -0.3705069059962831 1106 88-89 -0.2606324725072362 1106 90-91 -0.19088914289659087 1106 92-93 -0.38317457147189415 1106 94-95 -0.04286685802787815 1106 96-97 -0.3566768418040489 1106 98-99 0.003915981066882068 1106 100-101 0.2537808249008364 1107 1 -0.27235511431617354 1107 2 -0.10140265342337429 1107 3 -0.05430416415470418 1107 4 -0.17209942700669956 1107 5 -0.20286080972417153 1107 6 -0.41952211495532765 1107 7 -0.4089467800334816 1107 8 -0.4381942527819973 1107 9 -0.3889978010278412 1107 10-11 -0.32861193684450285 1107 12-13 -0.2874901377856034 1107 14-15 -0.06747142341141199 1107 16-17 -0.030069007316043894 1107 18-19 0.2736816964896178 1107 20-21 0.026906982636745624 1107 22-23 0.3296579282034031 1107 24-25 -0.011866858964914684 1107 26-27 -0.1380269379371768 1107 28-29 -0.2449525660879459 1107 30-31 -0.022073474540988514 1107 32-33 -0.06129650165603806 1107 34-35 0.09516049866746101 1107 36-37 -0.37896649034809116 1107 38-39 0.06023745621780563 1107 40-41 -0.10628498922649499 1107 42-43 0.09273696006473386 1107 44-45 -0.11428277806841436 1107 46-47 -0.39141746152454004 1107 48-49 0.3887531057032305 1107 50-51 0.2157262074724926 1107 52-53 0.3899474626332804 1107 54-55 0.3060775766560795 1107 56-57 0.3700339058510096 1107 58-59 0.34725445286038337 1107 60-61 0.043597982733921015 1107 62-63 0.842358785027379 1107 64-65 0.8730626987001102 1107 66-67 0.2080517671251414 1107 68-69 0.3368516395183718 1107 70-71 0.48998852203625276 1107 72-73 0.7234482833222522 1107 74-75 0.631578869144878 1107 76-77 0.3374974389839913 1107 78-79 0.4715717164182749 1107 80-81 0.22225985946660032 1107 82-83 0.13533100270008802 1107 84-85 0.44173612686729413 1107 86-87 0.4266444073592517 1107 88-89 0.41258247384866564 1107 90-91 0.29664322854254976 1107 92-93 0.5698775927900392 1107 94-95 0.5676029902888047 1107 96-97 1.0037745085474121 1107 98-99 0.791596336227812 1107 100-101 0.382637650650004 1108 1 -0.16265267565557195 1108 2 0.017979850864790592 1108 3 -0.08910168820571229 1108 4 0.019070682621162405 1108 5 0.21180098459307573 1108 6 0.05029666347707007 1108 7 0.14836547293094782 1108 8 0.10078702844702292 1108 9 0.1331887900676918 1108 10-11 0.1403119202663916 1108 12-13 0.16974753840204215 1108 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18 2168.0 19 1724.0 20 1792.0 21 1960.0 22 2254.0 23 2474.0 24 2930.0 25 3381.0 26 3881.0 27 4581.0 28 5359.0 29 6443.0 30 7749.0 31 9470.0 32 11319.0 33 14561.0 34 19158.0 35 28327.0 36 38155.0 37 47929.0 38 31718.0 39 2207.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.84269981727317 19.365639033174567 15.26771561198355 14.52394553756871 2 13.793780119174759 28.745721520515943 29.015166667295624 28.44533169301367 3 32.99051946679549 22.50116997026011 32.267024954333415 12.241285608610982 4 11.49872995384052 16.468705534230857 44.80753044902642 27.225034062902196 5 8.979624191271393 43.184408995855385 35.17790142004061 12.658065392832608 6 24.159668620837955 37.889042305167614 13.417008080762352 24.53428099323208 7 19.980458287119824 35.76661611474531 24.52975395549922 19.72317164263564 8 22.690952442033304 20.490368436540333 20.425856201851612 36.39282291957475 9 15.527805025277297 27.000679091526447 31.820719837018036 25.650796046178225 10-11 26.44501201582996 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21.11322524758093 36-37 21.583887123455877 22.98896967560879 36.269889545162556 19.157253655772777 38-39 20.379410878794253 29.01447512744591 31.261236173231715 19.344877820528122 40-41 19.664834519620136 31.26569667675547 28.514635079491953 20.554833724132447 42-43 18.178080660531965 30.64793290098652 22.56369158249924 28.610294855982282 44-45 22.451620726963355 22.78074947371612 26.73199976504338 28.035630034277137 46-47 27.862261312435816 29.74599361099848 28.110280380914848 14.281464695650856 48-49 23.004853200629913 26.37632108062832 29.793838462894147 20.824987255847624 50-51 12.311738711902434 29.478856371891364 37.85823650241414 20.35116841379206 52-53 20.43970037311241 33.46244147024102 23.434263808854265 22.66359434779231 54-55 16.753574527912203 24.788825265934268 29.4871551949553 28.97044501119823 56-57 24.55274942601896 22.63341744144636 27.144756882089517 25.669076250445165 58-59 31.16483891130178 22.862283841884956 26.27175786503021 19.701119381783055 60-61 21.92891613482193 27.40134761346705 34.333662127290594 16.33607412442043 62-63 17.05161846480301 28.217083637259265 33.02482877523374 21.70646912270398 64-65 16.784222614707065 35.38806728167189 27.367858523532668 20.459851580088383 66-67 21.764616696673933 26.126409551786224 23.190751445086704 28.91822230645314 68-69 17.496171225814276 26.16171074179657 29.570077940195304 26.772040092193848 70-71 27.13062662645967 26.654890135196354 24.25024118513352 21.964242053210466 72-73 22.459600161466696 20.818590653504227 36.37776383790411 20.344045347124965 74-75 16.90094399517863 35.49505260105638 26.70344602777214 20.900557375992843 76-77 19.90533809042903 25.606842335625352 26.40870222614647 28.079117347799148 78-79 21.37501326099147 26.42025218616916 30.231082248457934 21.97365230438143 80-81 26.1522431039709 24.628864807517427 26.80300848742043 22.41588360109124 82-83 21.18492329204727 23.094806368550593 34.56585212427124 21.154418215130896 84-85 20.462167518672768 27.79977793525259 30.453565351836204 21.284489194238443 86-87 20.04660900539035 29.662463645923133 28.22402637387622 22.066900974810295 88-89 19.043251867375595 29.133901535966167 23.52116359006037 28.301683006597873 90-91 22.429309688092797 22.77420980918685 26.696974922729105 28.099505579991245 92-93 26.61691353719735 28.21279127943116 27.737685531921347 17.432609651450143 94-95 23.031524044356924 25.412363611552813 28.96239592592522 22.593716418165037 96-97 15.513862830936 27.32635927757879 34.85720241817803 22.302575473307183 98-99 21.709368017481054 30.518689224249844 24.35388898470029 23.418053773568815 100-101 19.599587688249873 23.908965506787226 28.285988221829783 28.205458583133115 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2785.0 1 1594.5 2 362.0 3 293.0 4 223.0 5 203.5 6 335.5 7 677.0 8 871.5 9 801.5 10 597.0 11 373.5 12 326.0 13 328.0 14 309.5 15 363.5 16 502.5 17 464.0 18 331.0 19 305.0 20 304.0 21 307.0 22 329.5 23 388.0 24 448.0 25 561.5 26 808.5 27 1029.0 28 1079.0 29 1187.5 30 1481.5 31 1812.0 32 2164.5 33 2675.5 34 3282.5 35 3606.5 36 4018.5 37 4581.0 38 4566.5 39 4219.0 40 3665.5 41 3066.0 42 2669.5 43 2572.5 44 2596.0 45 3249.0 46 8188.5 47 28079.5 48 61631.5 49 59301.0 50 25136.0 51 7426.0 52 2802.0 53 1528.0 54 927.5 55 576.5 56 349.0 57 225.0 58 183.0 59 169.0 60 172.0 61 160.5 62 146.5 63 135.0 64 108.0 65 92.0 66 86.0 67 76.5 68 63.0 69 57.5 70 54.0 71 43.5 72 33.5 73 31.5 74 26.0 75 21.0 76 19.5 77 15.5 78 8.5 79 5.0 80 4.0 81 3.0 82 2.0 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 1.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.333692791481596 2 0.08483715037667695 3 0.09426350041852993 4 0.09916520244029349 5 0.11009976848884297 6 0.052787560234376765 7 0.052787560234376765 8 0.05580399224776973 9 0.05806631625781444 10-11 0.055426938246095604 12-13 0.053164614236050886 14-15 0.05127934422768028 16-17 0.052221979231865584 18-19 0.0620253832753927 20-21 0.06277949127874094 22-23 0.08012397535575046 24-25 0.09049296040178874 26-27 0.08314040736914341 28-29 0.0812551373607728 30-31 0.07409111132896454 32-33 0.06617297729380801 34-35 0.3227582254330465 36-37 0.5092114292608987 38-39 0.48206354114036215 40-41 0.4618911520507967 42-43 0.4922439991855634 44-45 0.503555619235787 46-47 0.4988424442148604 48-49 0.5156213472893588 50-51 0.5097770102634099 52-53 0.509965537264247 54-55 0.5026129842316016 56-57 0.47810447412278384 58-59 0.46339936805749316 60-61 0.42135784687082883 62-63 0.4926210531872375 64-65 0.5158098742901959 66-67 0.5237280083253524 68-69 0.5361707903805983 70-71 0.5325887773646941 72-73 0.5212771573144706 74-75 0.5235394813245152 76-77 0.500539187222394 78-79 0.48338323014622153 80-81 0.4879078781663109 82-83 0.4990309712156975 84-85 0.5003506602215569 86-87 0.4963915932039787 88-89 0.5056294162449946 90-91 0.516375455292707 92-93 0.519580414306937 94-95 0.5276870753429306 96-97 0.5199574683086111 98-99 0.5224083193194929 100-101 0.503744146236624 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 265214.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.82764861583476 #Duplication Level Percentage of deduplicated Percentage of total 1 72.4424666242848 10.741514399692324 2 9.813095994914177 2.910102784920856 3 4.559440559440559 2.02817347500509 4 2.8404322949777496 1.684677279479967 5 1.922441195168468 1.425264126328173 6 1.3986013986013985 1.2442782055245953 7 0.9688493324856962 1.0056030224648773 8 0.7857596948506039 0.9320774921384241 9 0.6408137317228226 0.8551584757969035 >10 4.144945963127782 11.346309018377612 >50 0.2390336935791481 2.4282277707813313 >100 0.13731722822631914 4.014871009826027 >500 0.027972027972027972 2.858823440693176 >1k 0.0508582326764145 18.54276169432986 >5k 0.020343293070565798 19.451084784362816 >10k+ 0.007628734901462174 18.53107302027796 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 23157 8.731439516767592 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15685 5.914092016258569 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10305 3.885541487251804 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7300 2.752494212221074 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7093 2.6744440338745314 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6754 2.546622727307005 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6520 2.458392090915261 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6457 2.4346376888097914 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6331 2.3871288845988525 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5614 2.1167811653985082 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5518 2.0805839812377926 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4619 1.7416124337327592 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4484 1.690710143506753 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4412 1.6635622553862164 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3737 1.4090508042561856 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3710 1.3988703462109844 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3405 1.2838688757003778 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2989 1.127014411003944 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2476 0.9335857081451204 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2250 0.8483715037667695 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2228 0.8400763157299389 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2140 0.8068955635826164 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1952 0.7360094112678818 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1908 0.7194190351942205 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1653 0.62327026476732 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1615 0.6089422127037034 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1377 0.5192033603052629 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1109 0.4181528878565989 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1098 0.41400529383818346 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1013 0.3819557036958833 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1003 0.3781851636791421 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 976 0.3680047056339409 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 942 0.35518486957702083 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 884 0.3333157374799219 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 728 0.2744953132187592 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 645 0.24319983107980725 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 606 0.22849472501451656 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 593 0.22359302299275302 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 590 0.22246186098773066 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 575 0.21680605096261887 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 526 0.19833040488058698 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 517 0.19493691886551992 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 463 0.17457600277511745 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 450 0.1696743007533539 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 425 0.1602479507115009 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 410 0.1545921406863891 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 405 0.1527068706780185 No Hit CCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 377 0.14214935863114314 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 300 0.11311620050223593 No Hit TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 275 0.10368985046038293 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 275 0.10368985046038293 No Hit GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT 268 0.1010504724486641 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.7705400167411976E-4 0.0 10-11 0.0 0.0 0.0 0.0011311620050223593 0.0 12-13 0.0 0.0 0.0 0.0011311620050223593 0.0 14-15 0.0 0.0 0.0 0.0011311620050223593 0.0 16-17 0.0 0.0 0.0 0.001696743007533539 0.0 18-19 0.0 0.0 0.0 0.0026393780117188385 0.0 20-21 0.0 0.0 0.0 0.0037705400167411976 0.0 22-23 0.0 0.0 0.0 0.005655810025111797 0.0 24-25 0.0 0.0 0.0 0.010368985046038294 0.0 26-27 0.0 0.0 0.0 0.02017238908956541 0.0 28-29 0.0 0.0 0.0 0.06315654528041506 0.0 30-31 0.0 0.0 0.0 0.14950191166378848 0.0 32-33 0.0 0.0 0.0 0.3056022683568741 0.0 34-35 0.0 0.0 0.0 0.4843258651504068 0.0 36-37 0.0 0.0 0.0 0.698869592102981 0.0 38-39 0.0 0.0 0.0 1.0227589795410499 0.0 40-41 0.0 0.0 0.0 1.3142217228351445 0.0 42-43 0.0 0.0 0.0 1.6330208812506126 0.0 44-45 0.0 0.0 0.0 2.029304637010113 0.0 46-47 0.0 0.0 0.0 2.4730971969805515 0.0 48-49 0.0 0.0 0.0 2.8077326234663325 0.0 50-51 0.0 0.0 0.0 3.1800734501195262 0.0 52-53 0.0 0.0 0.0 3.53506979269571 0.0 54-55 0.0 0.0 0.0 3.9690589486226218 0.0 56-57 0.0 0.0 0.0 4.608542535461929 0.0 58-59 0.0 0.0 0.0 5.067417255499333 0.0 60-61 0.0 0.0 0.0 5.591710844827196 0.0 62-63 0.0 0.0 0.0 6.136176823244625 0.0 64-65 0.0 0.0 0.0 6.688937989698885 0.0 66-67 0.0 0.0 0.0 7.390258432812747 0.0 68-69 0.0 0.0 0.0 8.206768873438055 0.0 70-71 0.0 0.0 0.0 8.98934445391269 0.0 72-73 0.0 0.0 0.0 9.637688809791339 0.0 74-75 0.0 0.0 0.0 10.572782733943155 0.0 76-77 0.0 0.0 0.0 11.291636188134865 0.0 78-79 0.0 0.0 0.0 12.602841478956616 0.0 80-81 0.0 0.0 0.0 13.759643156092816 0.0 82-83 0.0 0.0 0.0 14.63214611596673 0.0 84-85 0.0 0.0 0.0 15.710332033753875 0.0 86-87 0.0 0.0 0.0 16.718008853227957 0.0 88-89 0.0 0.0 0.0 17.897245243463768 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACAAGA 20 0.0020907843 71.090744 4 AAGACGC 20 0.0020939175 71.06392 7 CAAGACG 20 0.0020939175 71.06392 6 TCCTGGA 30 1.3138508E-4 63.191772 2 CATATAG 30 1.3163073E-4 63.167927 9 AGACATA 30 1.3163073E-4 63.167927 6 GACATAT 40 7.647157E-6 59.219933 7 CTATATG 25 0.005061655 56.872593 3 AGACGCC 25 0.005069224 56.851128 8 AAGACAT 35 2.8149562E-4 54.164375 5 GTCCTGG 40 5.3999206E-4 47.465504 1 GAGGAGA 40 5.45045E-4 47.375946 7 ACATATA 40 5.45045E-4 47.375946 8 AAAGACA 45 9.718076E-4 42.127846 4 GTAAGCG 140 0.0 39.05597 94-95 TCTGGAC 25 0.0016043099 38.05152 34-35 CGTGGGA 25 0.0016058037 38.04432 66-67 CCCAGTC 25 0.0016072986 38.037113 42-43 CCAGTCT 25 0.0016072986 38.037113 42-43 CTGGACC 25 0.0016080465 38.033512 36-37 >>END_MODULE