##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938915_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 265214 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.93186257135747 31.0 31.0 34.0 30.0 34.0 2 32.136169282164595 33.0 31.0 34.0 30.0 34.0 3 32.236665485230795 34.0 31.0 34.0 30.0 34.0 4 35.725478293001125 37.0 35.0 37.0 35.0 37.0 5 35.66772116102468 37.0 35.0 37.0 33.0 37.0 6 35.81457615359672 37.0 35.0 37.0 35.0 37.0 7 35.78739810115605 37.0 35.0 37.0 33.0 37.0 8 35.82957913232333 37.0 35.0 37.0 33.0 37.0 9 37.356059634860905 39.0 37.0 39.0 34.0 39.0 10-11 37.23557014335593 39.0 37.0 39.0 33.5 39.0 12-13 37.24522272579879 39.0 37.0 39.0 33.0 39.0 14-15 38.192844269156225 40.0 37.5 41.0 33.5 41.0 16-17 38.476366255175066 40.0 38.0 41.0 34.0 41.0 18-19 38.57349536600632 40.0 38.0 41.0 34.0 41.0 20-21 38.505465397754264 40.0 38.0 41.0 34.0 41.0 22-23 38.45479122519927 40.0 38.0 41.0 34.0 41.0 24-25 38.224458361926594 40.0 37.5 41.0 33.5 41.0 26-27 37.948933691283266 39.5 37.0 41.0 33.0 41.0 28-29 37.9196837271034 40.0 37.0 41.0 33.0 41.0 30-31 37.93725444358141 40.0 37.0 41.0 33.0 41.0 32-33 37.75719984616197 40.0 37.0 41.0 33.0 41.0 34-35 37.4736722043331 39.5 36.0 41.0 32.5 41.0 36-37 37.26919958976525 39.5 36.0 41.0 32.0 41.0 38-39 37.07962060826351 39.0 36.0 41.0 31.0 41.0 40-41 37.30408462600013 40.0 36.0 41.0 32.0 41.0 42-43 37.01301024832777 39.5 36.0 41.0 31.0 41.0 44-45 36.83112882427021 39.0 36.0 41.0 31.0 41.0 46-47 36.5659014984126 39.0 35.0 41.0 30.0 41.0 48-49 36.151958418484696 39.0 35.0 40.0 29.0 41.0 50-51 35.87054793487523 39.0 35.0 40.0 28.0 41.0 52-53 35.886638714396675 39.0 35.0 40.0 28.0 41.0 54-55 35.77873528546759 38.5 35.0 40.0 28.0 41.0 56-57 35.43114616875429 38.5 34.5 40.0 27.5 41.0 58-59 35.152429735986786 38.0 34.0 40.0 27.0 41.0 60-61 34.40715233735776 38.0 33.5 40.0 26.0 41.0 62-63 34.0927251200917 37.0 33.0 40.0 25.5 41.0 64-65 33.712351157932844 36.5 32.5 39.0 25.0 40.5 66-67 32.946850467924016 36.0 32.0 39.0 22.5 40.0 68-69 32.253186106314146 35.5 31.5 37.5 21.5 39.0 70-71 31.32319560807499 34.5 30.5 37.0 19.5 38.5 72-73 30.620836381186514 34.0 29.5 36.0 16.5 37.0 74-75 30.218018279578 34.0 29.0 35.5 14.5 37.0 76-77 28.39713778307329 31.5 27.5 34.0 11.0 35.5 78-79 29.374733234293814 33.5 29.0 35.0 7.5 36.0 80-81 29.2230180156402 34.0 29.0 35.0 7.0 36.0 82-83 28.74596552218209 33.5 28.5 35.0 2.0 35.0 84-85 28.222522189628 33.0 27.5 35.0 2.0 35.0 86-87 27.893146666465572 33.0 27.0 35.0 2.0 35.0 88-89 27.076900163641437 32.0 25.5 34.0 2.0 35.0 90-91 26.384842052078696 32.0 25.0 34.0 2.0 35.0 92-93 26.041187493872876 32.0 24.0 34.0 2.0 35.0 94-95 25.377904258447895 31.0 23.0 34.0 2.0 35.0 96-97 24.271716425226423 31.0 15.5 34.0 2.0 35.0 98-99 23.12080433159637 31.0 2.0 34.0 2.0 35.0 100-101 17.46569374165768 23.5 2.0 30.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14656809450584873 1101 2 0.19065697442195528 1101 3 0.07508472558535573 1101 4 0.003896294222172969 1101 5 0.010804339820687403 1101 6 0.17646122555810706 1101 7 0.17538474827862416 1101 8 -0.05955550509323615 1101 9 0.06240076437338615 1101 10-11 0.12836098210837577 1101 12-13 0.11395499499882078 1101 14-15 -0.04539948167538199 1101 16-17 0.04686735172734302 1101 18-19 0.17627914852751303 1101 20-21 0.02968653776775909 1101 22-23 0.12022000392118315 1101 24-25 0.07136434973560313 1101 26-27 -0.13458305539369064 1101 28-29 0.11678222050307596 1101 30-31 -0.15007620408540845 1101 32-33 0.19529938913132838 1101 34-35 0.29352196457695356 1101 36-37 -0.029987493955594857 1101 38-39 -0.09600752428027448 1101 40-41 -0.40428147930089153 1101 42-43 -0.18069791426989923 1101 44-45 -0.23160204890081815 1101 46-47 -0.4162179572264293 1101 48-49 -0.20822692359484307 1101 50-51 -0.025899458636281736 1101 52-53 -0.335047738047777 1101 54-55 -0.49280832037498357 1101 56-57 -0.5776891157536284 1101 58-59 -0.3604934865489753 1101 60-61 -0.8390562851451477 1101 62-63 -0.6331721774679906 1101 64-65 -0.22771035022997665 1101 66-67 -0.32969079165408033 1101 68-69 -0.5251716472220878 1101 70-71 -0.4277573361139204 1101 72-73 -0.21499106315928174 1101 74-75 -0.14518331735037293 1101 76-77 -0.22154764403762783 1101 78-79 0.11460955545018336 1101 80-81 0.45209741666152325 1101 82-83 -0.06551964493029061 1101 84-85 0.2650005943080096 1101 86-87 0.20490436911962462 1101 88-89 0.6910053376933334 1101 90-91 0.008063628536490342 1101 92-93 0.3140649521115435 1101 94-95 0.12887068943015123 1101 96-97 0.8840338143767319 1101 98-99 0.8517804806803575 1101 100-101 0.705325601513028 1102 1 -0.03966706901562844 1102 2 -0.039639328199505997 1102 3 0.04511290422082226 1102 4 0.02621718910732085 1102 5 0.12425323402272426 1102 6 -0.06621659870967278 1102 7 0.291617350105966 1102 8 0.1025826556103695 1102 9 0.25369100431873903 1102 10-11 0.3095923030886496 1102 12-13 0.3473851034438695 1102 14-15 0.4609449807958015 1102 16-17 0.4832434084261692 1102 18-19 0.3198962606443274 1102 20-21 0.4060591788744077 1102 22-23 0.4680724503390863 1102 24-25 0.6007384424689306 1102 26-27 0.5556739679177269 1102 28-29 0.6148011770396664 1102 30-31 0.5213523673431624 1102 32-33 0.7600504778513368 1102 34-35 0.8307519193203561 1102 36-37 1.0647439551093854 1102 38-39 0.8485830316073546 1102 40-41 0.6996037568019204 1102 42-43 0.8723547712394506 1102 44-45 0.8046886179935555 1102 46-47 1.0563975324841053 1102 48-49 0.9001498162224308 1102 50-51 0.6927325966963096 1102 52-53 1.0339301459928691 1102 54-55 1.0485032670214878 1102 56-57 0.6470653885585591 1102 58-59 0.2628691639505547 1102 60-61 0.6258997391055559 1102 62-63 0.6219042464070057 1102 64-65 0.8496442736279306 1102 66-67 0.16552737929631434 1102 68-69 -0.014070972642635127 1102 70-71 0.04565227877806066 1102 72-73 0.6500930457981084 1102 74-75 0.8797761224925118 1102 76-77 0.8090389839189953 1102 78-79 0.6346163866345371 1102 80-81 0.8123057677843981 1102 82-83 0.334437660167481 1102 84-85 0.6937684194296914 1102 86-87 0.9919848917052292 1102 88-89 0.6709814285480853 1102 90-91 0.496424998188953 1102 92-93 0.6918465249917389 1102 94-95 0.7482797919833075 1102 96-97 0.980549910354874 1102 98-99 0.7354454110876674 1102 100-101 1.7965816855365944 1103 1 0.019480895990074742 1103 2 -0.11521248079814939 1103 3 -0.235169954799197 1103 4 -0.17309813991511902 1103 5 0.10608178964359638 1103 6 -0.046679270309660126 1103 7 -0.09366568181076929 1103 8 -0.058003810035053505 1103 9 -0.45264393119078505 1103 10-11 -0.08586570030012552 1103 12-13 -0.12511567459133488 1103 14-15 0.006287143387872618 1103 16-17 -0.18393042192757747 1103 18-19 -0.26003388908421954 1103 20-21 -0.324749286148581 1103 22-23 -0.2916875690253491 1103 24-25 0.09826601949441027 1103 26-27 0.0017215490927284804 1103 28-29 -0.2003117245129502 1103 30-31 -0.019969946706424935 1103 32-33 -0.0051644327610631535 1103 34-35 0.1528152686754538 1103 36-37 0.09323733316534089 1103 38-39 -0.3082861547407205 1103 40-41 -0.4686852573410363 1103 42-43 -0.35801280716921013 1103 44-45 -0.4636816576038072 1103 46-47 0.06096200566782528 1103 48-49 -0.23735493843714295 1103 50-51 -0.17826916685014993 1103 52-53 -0.21379626225084536 1103 54-55 0.16395504612864897 1103 56-57 0.47234461674763395 1103 58-59 0.16839840078446855 1103 60-61 0.4824819169125334 1103 62-63 0.5630329161397043 1103 64-65 0.0553222016337358 1103 66-67 -0.3155231411227888 1103 68-69 -0.30274796700620143 1103 70-71 -0.5235323403304832 1103 72-73 -0.5750011829096628 1103 74-75 -0.48306491627092996 1103 76-77 -0.681650528166486 1103 78-79 -0.5294366580765519 1103 80-81 -0.6427583768955678 1103 82-83 -0.5405407277435827 1103 84-85 -0.1751326490719869 1103 86-87 -0.008706695140787701 1103 88-89 0.15322493627656897 1103 90-91 0.034341247021899335 1103 92-93 6.141172321392219E-4 1103 94-95 0.07006650659999636 1103 96-97 -0.21190141922083683 1103 98-99 0.3459869235881001 1103 100-101 0.42084255209491417 1104 1 -0.06316373210909632 1104 2 0.09139908944978714 1104 3 -0.18145094653473137 1104 4 0.14921590967165343 1104 5 -0.07160416076963116 1104 6 0.031833126384555044 1104 7 -0.011021053711594675 1104 8 0.008111892444290447 1104 9 -0.04355302209987855 1104 10-11 0.31289463027838593 1104 12-13 0.4678595320202348 1104 14-15 0.7005020194209308 1104 16-17 0.42763982600631323 1104 18-19 0.18418098694883867 1104 20-21 0.25582922624811033 1104 22-23 0.2227752408920054 1104 24-25 0.26768750709772604 1104 26-27 0.18973807801835108 1104 28-29 0.23977092011515566 1104 30-31 0.06680691279770912 1104 32-33 -0.09607352367015665 1104 34-35 0.11355907032834978 1104 36-37 0.10150179597525977 1104 38-39 0.020226241953487545 1104 40-41 -0.2724042656055019 1104 42-43 -0.41793016254110427 1104 44-45 -0.5050039716533874 1104 46-47 -0.1973024571420936 1104 48-49 -0.1567764260404445 1104 50-51 -0.3952113156104744 1104 52-53 -0.36668882423431626 1104 54-55 -0.35670611089614823 1104 56-57 -0.0793082758143413 1104 58-59 -0.21589911987669552 1104 60-61 0.11884555327616653 1104 62-63 -0.35019022435616165 1104 64-65 -0.5025290380356822 1104 66-67 -0.36097768657733553 1104 68-69 -0.5093595372541344 1104 70-71 0.12729410595050794 1104 72-73 0.18532939560273576 1104 74-75 -0.4169492137915931 1104 76-77 -0.1444604455218581 1104 78-79 0.07593524274989605 1104 80-81 0.3221176561622805 1104 82-83 -0.03227626493366387 1104 84-85 -0.24538058295628318 1104 86-87 0.2268304949418578 1104 88-89 0.21934063875590937 1104 90-91 0.449630503220245 1104 92-93 0.6307794064883367 1104 94-95 1.210562374368589 1104 96-97 1.5112390766469304 1104 98-99 1.1497059318525622 1104 100-101 1.371255775235408 1105 1 -0.05173122522204565 1105 2 0.18912636217706336 1105 3 0.09396227660576528 1105 4 0.19811398129699853 1105 5 0.22092338743973983 1105 6 0.18479455889143992 1105 7 0.24128610882284107 1105 8 0.06527442687954732 1105 9 0.10100620655025949 1105 10-11 0.0634800297274225 1105 12-13 0.4198223419375964 1105 14-15 0.2706294298892473 1105 16-17 0.19580442655728092 1105 18-19 0.18886418254111703 1105 20-21 0.35417633368613366 1105 22-23 0.16826422160826127 1105 24-25 0.08671298919138337 1105 26-27 0.1368455160348816 1105 28-29 0.347777118462254 1105 30-31 0.39010236734316095 1105 32-33 0.38896435511772154 1105 34-35 0.6001121006313781 1105 36-37 0.9111264516226427 1105 38-39 0.27363533286258246 1105 40-41 0.15970661593719626 1105 42-43 0.3664534462743134 1105 44-45 0.6436530531399924 1105 46-47 1.0287650359708564 1105 48-49 1.0164329403507324 1105 50-51 0.9402570039487514 1105 52-53 1.0694165476665063 1105 54-55 1.2178634483325013 1105 56-57 1.546673129144331 1105 58-59 1.6797276018863982 1105 60-61 1.6401954155351248 1105 62-63 0.9250336048449341 1105 64-65 0.8659971327632192 1105 66-67 1.2170353988221123 1105 68-69 1.3055681486962811 1105 70-71 1.835593004022133 1105 72-73 1.823741930037997 1105 74-75 1.8189323289081365 1105 76-77 1.3684183423569287 1105 78-79 1.3843374466066436 1105 80-81 1.1621314302669639 1105 82-83 1.5882643686751514 1105 84-85 1.7774155602944042 1105 86-87 1.7216135527930945 1105 88-89 1.6761243853123844 1105 90-91 1.5779190707133601 1105 92-93 2.1803489657169877 1105 94-95 2.5432414377294705 1105 96-97 2.4186426579141482 1105 98-99 2.898294086122533 1105 100-101 2.4536249212409196 1106 1 0.013955049287758925 1106 2 -0.19469716723470398 1106 3 0.004256394252820428 1106 4 -0.2009056265461382 1106 5 -0.051380534128888655 1106 6 0.10195142163653514 1106 7 -0.1486648715693164 1106 8 0.12899991707562464 1106 9 -0.233797715018369 1106 10-11 -0.055392519292183806 1106 12-13 -0.17159922668985672 1106 14-15 -0.37361076618918077 1106 16-17 -0.33152400481527167 1106 18-19 -0.2376064056941729 1106 20-21 -0.03798052905897009 1106 22-23 0.19645049611806797 1106 24-25 -0.06537034414194665 1106 26-27 -0.18472311141609765 1106 28-29 0.26039966748187027 1106 30-31 0.1919406026372812 1106 32-33 0.27977317864713314 1106 34-35 -0.09339280132940786 1106 36-37 0.1475235104461703 1106 38-39 -0.16435486321585557 1106 40-41 0.23017230221171303 1106 42-43 0.24035050509784384 1106 44-45 0.489486386473331 1106 46-47 0.40106895753947924 1106 48-49 0.32574666584093137 1106 50-51 0.590747200027181 1106 52-53 0.1153724300194483 1106 54-55 0.3341624679403452 1106 56-57 -1.8047326257430996E-5 1106 58-59 -0.4518900451724335 1106 60-61 -0.09411830995507842 1106 62-63 0.1729502715116027 1106 64-65 0.005703015116161225 1106 66-67 -0.2705871501974926 1106 68-69 -0.25754459640175753 1106 70-71 -0.007789348919043704 1106 72-73 3.3506729289456416E-4 1106 74-75 -0.022734337758535617 1106 76-77 -0.32226793215287586 1106 78-79 0.030415877979191208 1106 80-81 -0.4314960207134284 1106 82-83 -0.25303465093269395 1106 84-85 -0.3583687534310833 1106 86-87 0.0342361018127022 1106 88-89 0.5954871304104223 1106 90-91 0.5706886785564969 1106 92-93 0.09909896571698695 1106 94-95 -0.09621934658425246 1106 96-97 -0.47719067541918747 1106 98-99 -0.3867549334853102 1106 100-101 0.634017078103664 1107 1 -0.1765739925176426 1107 2 -0.01889250574746626 1107 3 -0.049905647922535934 1107 4 -0.18270362876589274 1107 5 0.04961835599319642 1107 6 0.14249896140716345 1107 7 0.07627038555239807 1107 8 0.0655888922883534 1107 9 0.277264068185481 1107 10-11 0.06340141337521743 1107 12-13 -0.024281431647310114 1107 14-15 0.0757788009584246 1107 16-17 0.11298209951325333 1107 18-19 -0.03346286148403266 1107 20-21 -0.0043142323516107695 1107 22-23 0.29522963041328865 1107 24-25 -0.06725713659478316 1107 26-27 0.03873230848771669 1107 28-29 -0.031350240028309884 1107 30-31 -0.054119330770042495 1107 32-33 0.15598479536929233 1107 34-35 0.23568600000244544 1107 36-37 0.0833355711194983 1107 38-39 0.023399483805981447 1107 40-41 0.045044666251669696 1107 42-43 0.08811225130576616 1107 44-45 0.10898324181923869 1107 46-47 0.11705119949287024 1107 48-49 0.2269675315457036 1107 50-51 -0.024051172151878575 1107 52-53 0.3978363589872629 1107 54-55 0.5930599892130033 1107 56-57 0.4548099215971604 1107 58-59 0.522101815722877 1107 60-61 0.2776167991829226 1107 62-63 0.1729502715116027 1107 64-65 0.14602857930409385 1107 66-67 0.17084829190387296 1107 68-69 0.07101626190382149 1107 70-71 0.40123765811018686 1107 72-73 0.07110828223925125 1107 74-75 -0.07745131889060985 1107 76-77 0.1959340656273696 1107 78-79 0.4884254969211099 1107 80-81 0.29121948058142877 1107 82-83 0.07737600389527799 1107 84-85 0.04644071752710843 1107 86-87 0.0028635527930944704 1107 88-89 0.12227218405451978 1107 90-91 0.773870328574997 1107 92-93 0.8984700348993755 1107 94-95 1.0598294880439347 1107 96-97 1.0454508340147761 1107 98-99 0.5908255326634162 1107 100-101 1.209599764008214 1108 1 0.12422397899402782 1108 2 0.1289484965644121 1108 3 -0.03736183406617499 1108 4 0.01662518024298265 1108 5 0.12746159560838066 1108 6 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2316 66-67 -0.933838257091864 2316 68-69 -1.0990689480779139 2316 70-71 -0.6427672110316287 2316 72-73 -0.8741747366286745 2316 74-75 -1.0904762732424054 2316 76-77 -1.1044311200086625 2316 78-79 -1.2742647039309922 2316 80-81 -1.1666320105932506 2316 82-83 -1.1245716528302232 2316 84-85 -1.6099500311034447 2316 86-87 -1.624555802045613 2316 88-89 -1.4076121738274026 2316 90-91 -1.5708040475662095 2316 92-93 -1.5272451203045208 2316 94-95 -1.1012478095823575 2316 96-97 -1.1707390625159633 2316 98-99 -1.3615177418344544 2316 100-101 -0.9678804551031668 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 4.0 5 18.0 6 34.0 7 66.0 8 93.0 9 170.0 10 313.0 11 423.0 12 615.0 13 766.0 14 1021.0 15 1282.0 16 1710.0 17 2223.0 18 2197.0 19 1828.0 20 1996.0 21 2137.0 22 2426.0 23 2575.0 24 3058.0 25 3480.0 26 4088.0 27 4566.0 28 5695.0 29 6798.0 30 8239.0 31 10043.0 32 12364.0 33 15815.0 34 21358.0 35 30366.0 36 44030.0 37 52606.0 38 20343.0 39 467.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.790245269159996 20.32992828962812 15.649065947796062 14.230760493415822 2 13.929505983847006 29.377785486437368 28.66590753127663 28.026800998438993 3 33.18037509332086 22.534632410053767 32.36744666571146 11.917545830913904 4 11.474884432948487 17.279253734719887 43.948660327132046 27.297201505199574 5 8.703537520643707 44.059514203624246 35.029447917530746 12.207500358201303 6 24.091865436967883 38.65482214362741 12.937853959444071 24.315458459960634 7 19.984616196731697 35.904967309418055 23.89579735609734 20.21461913775291 8 23.09757403455323 21.002284947250143 19.802876167924772 36.097264850271856 9 15.244670341686337 27.368841765517658 31.039462471815217 26.347025420980792 10-11 26.51971615374754 22.508615683938256 25.503744146236624 25.467924016077582 12-13 34.64975453784491 22.704495237807958 23.903338436130824 18.74241178821631 14-15 23.70173880747557 28.293940096640213 33.01660379208214 14.987717303802075 16-17 17.089131422516914 30.272859758040344 31.538085928294425 21.099922891148317 18-19 16.67832398548334 37.352311825423016 26.64919639911392 19.320167789979735 20-21 22.765766513080006 26.488043617606916 22.602313603354276 28.143876265958813 22-23 18.055796451167737 27.191437857729984 28.94304222250711 25.809723468595173 24-25 28.537574444739803 27.864531782884356 23.400203986599475 20.197689785776365 26-27 23.505923518366302 21.316747984646362 37.01689956035503 18.160428936632307 28-29 16.769476724456478 37.55665236375154 26.67336565942974 19.00050525236224 30-31 20.27287398101156 26.393214536185873 25.65814775992218 27.675763722880394 32-33 21.919845860324116 27.85656111668313 30.351527445760784 19.87206557723197 34-35 27.62402709062387 24.990949680222034 26.70636237480391 20.678660854350188 36-37 21.517020566478546 23.61779197441588 35.8483130283277 19.016874430777875 38-39 20.21852256872431 29.3274633281798 31.26475357290999 19.189260530185905 40-41 19.632393420864098 31.445841283248242 28.14816630342154 20.77359899246612 42-43 18.20106598190433 31.072976585728213 22.52966140840078 28.19629602396668 44-45 22.72180536178877 22.9156140417028 26.344971909053207 28.017608687455226 46-47 28.016130522243564 30.02660141057232 27.817421190257583 14.139846876926528 48-49 23.354775703507073 26.511555124864728 29.486778252459402 20.6468909191688 50-51 12.225267992142165 29.835830068661796 37.625983643335736 20.312918295860307 52-53 20.48327011394572 33.874720037403755 23.211067289057137 22.430942559593383 54-55 16.815665839661555 25.145919898647882 28.888369392264362 29.1500448694262 56-57 24.647831562436373 23.27007624031914 26.702021763556978 25.380070433687514 58-59 31.163878022340576 23.394824904557666 25.826271386152616 19.615025686949146 60-61 21.718892280312126 28.679940349494647 33.60732849065746 15.993838879535765 62-63 16.867388107537423 29.119754157083065 32.1548961200558 21.857961615323706 64-65 16.364328779726332 36.528462243270454 26.52435230816218 20.582856668841035 66-67 21.782107285399977 26.833227441805217 22.353544475384464 29.03112079741034 68-69 17.548579519210282 27.0124184842549 28.50274971296277 26.936252283572042 70-71 26.972746536758997 27.41672762373027 23.504415302359604 22.10611053715113 72-73 22.526337222016938 21.32504317268319 35.62613587518004 20.522483730119827 74-75 16.501391329266177 36.06973990814964 25.920388818086526 21.50847994449765 76-77 19.79095707241777 25.9677057170731 25.609739988942128 28.631597221567013 78-79 21.04368122078916 27.000194189034545 29.484305566815166 22.471819023361128 80-81 25.96700394210702 24.82415110844659 26.054480635412602 23.15436431403379 82-83 20.62749326958607 23.66824526608701 33.97407376684489 21.730187697482034 84-85 19.72354348477736 28.17409370186661 29.921742294415633 22.1806205189404 86-87 19.21918635368707 30.18529700354744 27.46395954411654 23.131557098648955 88-89 18.19601651550174 29.45415925986926 22.793247651122332 29.556576573506664 90-91 21.91221188433934 22.721140182070364 26.168972348843944 29.197675584746356 92-93 25.518449252301917 28.59577548696524 27.371669670530213 18.51410559020263 94-95 22.206134372507876 25.39152861594846 28.747353517300407 23.65498349424325 96-97 13.80488963629371 28.10937582480563 34.6375002827905 23.448234256110158 98-99 20.098109451235608 30.693138371277534 23.867895359973456 25.3408568175134 100-101 18.17403714365973 24.74817458387299 27.488985289210394 29.588802983256883 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2522.0 1 1480.0 2 370.5 3 275.0 4 217.0 5 211.0 6 332.5 7 643.0 8 808.5 9 720.5 10 537.5 11 339.0 12 286.0 13 286.0 14 253.0 15 232.0 16 236.5 17 238.0 18 243.0 19 232.5 20 248.0 21 275.0 22 288.0 23 336.5 24 395.5 25 458.5 26 637.5 27 884.5 28 985.5 29 1112.5 30 1388.5 31 1701.0 32 2120.5 33 2726.5 34 3347.5 35 3749.0 36 4241.0 37 4700.5 38 4689.0 39 4294.0 40 3731.0 41 3217.0 42 2795.0 43 2689.5 44 2756.0 45 3395.5 46 7219.0 47 27642.5 48 61112.5 49 59277.5 50 28176.0 51 10212.0 52 3284.0 53 836.5 54 324.5 55 187.0 56 112.5 57 75.0 58 58.0 59 59.5 60 52.0 61 39.0 62 41.5 63 33.0 64 17.5 65 11.0 66 10.5 67 14.0 68 12.0 69 8.5 70 6.0 71 6.5 72 6.0 73 3.0 74 3.0 75 2.5 76 1.0 77 0.5 78 0.5 79 0.0 80 0.5 81 1.5 82 1.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4132511858348353 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013196890058594193 16-17 0.001696743007533539 18-19 5.655810025111796E-4 20-21 0.0 22-23 0.0 24-25 1.8852700083705988E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011311620050223592 36-37 0.01866417308286893 38-39 0.009049296040178874 40-41 0.004524648020089437 42-43 0.004713175020926497 44-45 0.001508216006696479 46-47 9.426350041852994E-4 48-49 0.0026393780117188385 50-51 3.7705400167411976E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.655810025111796E-4 60-61 9.426350041852994E-4 62-63 0.001508216006696479 64-65 0.0011311620050223593 66-67 0.0020737970092076586 68-69 0.001696743007533539 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09218970340932228 78-79 0.0032049590142300176 80-81 1.8852700083705988E-4 82-83 0.0 84-85 1.8852700083705988E-4 86-87 0.08823063639174403 88-89 0.41419382083902057 90-91 0.36781617863310384 92-93 0.0 94-95 0.0013196890058594193 96-97 0.0 98-99 0.0 100-101 1.8852700083705988E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 265214.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.63658781210645 #Duplication Level Percentage of deduplicated Percentage of total 1 67.84030554395179 8.572699782062788 2 11.523542400190966 2.912365108930901 3 5.320164707286508 2.0168618549548665 4 3.2105985558274153 1.6228404232054114 5 2.1035984961508625 1.329115355901272 6 1.670943486304231 1.2669014456250425 7 1.1845795786835354 1.0478330706523789 8 0.978695470549621 0.9893897003928903 9 0.7399892582204453 0.8415845317366354 >10 4.8546875932446145 11.51636037313264 >50 0.26854448887032284 2.3663909145067756 >100 0.1790296592468819 4.575550310315443 >500 0.03282210419526168 3.013038527377891 >1k 0.05967655308229397 18.779928661382883 >5k 0.026854448887032287 24.221194959542107 >10k+ 0.0059676553082293965 14.927944980280076 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24467 9.225380258960689 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15124 5.702564721319387 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9938 3.747162668637402 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7867 2.9662838311703 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7323 2.761166454259579 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7186 2.7095100560302248 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7011 2.6435256057372536 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6937 2.615623609613369 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6595 2.48667114104082 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5793 2.184273831698176 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5588 2.1069777613549814 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4881 1.8404005821713785 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4524 1.705792303573718 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4197 1.5824956450262808 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3989 1.5040684126780637 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3778 1.4245100183248245 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3286 1.2389994495011574 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2838 1.070079256751152 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2583 0.9739304863242513 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2396 0.903421388011191 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2185 0.8238629936579516 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2158 0.8136825356127505 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2135 0.8050102935742457 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1909 0.7197960891958947 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1579 0.5953682686434352 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1438 0.5422036544073843 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1392 0.5248591703303748 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1291 0.48677671616128865 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1101 0.41513645584320585 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1084 0.4087265378147458 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1063 0.4008084037795894 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 960 0.36197184160715495 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 944 0.355938977580369 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 934 0.35216843756362787 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 847 0.31936473941797944 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 722 0.27223298920871447 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 644 0.24282277707813313 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 630 0.23754402105469544 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 624 0.2352816970446507 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 579 0.21831426696931533 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 559 0.21077318693583294 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 548 0.20662559291741764 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 496 0.1870187848303634 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 490 0.18475646082031869 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 480 0.18098592080357748 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 472 0.1779694887901845 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 470 0.1772153807868363 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 417 0.15723151869810795 TruSeq Adapter, Index 3 (95% over 21bp) CCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 383 0.14441168264118787 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 379 0.14290346663449138 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 325 0.12254255054408893 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 291 0.10972271448716886 No Hit GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT 283 0.10670628247377589 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 271 0.10218163445368646 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.7705400167411976E-4 0.0 10-11 0.0 0.0 0.0 0.0011311620050223593 0.0 12-13 0.0 0.0 0.0 0.0011311620050223593 0.0 14-15 0.0 0.0 0.0 0.0011311620050223593 0.0 16-17 0.0 0.0 0.0 0.001696743007533539 0.0 18-19 0.0 0.0 0.0 0.0022623240100447185 0.0 20-21 0.0 0.0 0.0 0.003393486015067078 0.0 22-23 0.0 0.0 0.0 0.005844337025948856 0.0 24-25 0.0 0.0 0.0 0.011311620050223594 0.0 26-27 0.0 0.0 0.0 0.021115024093750705 0.0 28-29 0.0 0.0 0.0 0.06447623428627448 0.0 30-31 0.0 0.0 0.0 0.15195276267467026 0.0 32-33 0.0 0.0 0.0 0.3095613353744523 0.0 34-35 0.0 0.0 0.0 0.49054725617802986 0.0 36-37 0.0 0.0 0.0 0.7056565641331152 0.0 38-39 0.0 0.0 0.0 1.029734478572021 0.0 40-41 0.0 0.0 0.0 1.3240251268786716 0.0 42-43 0.0 0.0 0.0 1.6454636633058586 0.0 44-45 0.0 0.0 0.0 2.04419827007624 0.0 46-47 0.0 0.0 0.0 2.4917613700634202 0.0 48-49 0.0 0.0 0.0 2.82847059355841 0.0 50-51 0.0 0.0 0.0 3.2021311092174622 0.0 52-53 0.0 0.0 0.0 3.5586356678003424 0.0 54-55 0.0 0.0 0.0 3.997903579750692 0.0 56-57 0.0 0.0 0.0 4.641723287609251 0.0 58-59 0.0 0.0 0.0 5.101729169651678 0.0 60-61 0.0 0.0 0.0 5.628473609990423 0.0 62-63 0.0 0.0 0.0 6.177652763428778 0.0 64-65 0.0 0.0 0.0 6.732487726892245 0.0 66-67 0.0 0.0 0.0 7.437013129020338 0.0 68-69 0.0 0.0 0.0 8.259556433672431 0.0 70-71 0.0 0.0 0.0 9.047787824172179 0.0 72-73 0.0 0.0 0.0 9.70103388207259 0.0 74-75 0.0 0.0 0.0 10.642349197252031 0.0 76-77 0.0 0.0 0.0 11.36308792145211 0.0 78-79 0.0 0.0 0.0 12.676178482282232 0.0 80-81 0.0 0.0 0.0 13.833168686419269 0.0 82-83 0.0 0.0 0.0 14.704351957287322 0.0 84-85 0.0 0.0 0.0 15.778767335057726 0.0 86-87 0.0 0.0 0.0 16.78606710053014 0.0 88-89 0.0 0.0 0.0 17.973221624801106 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAAGCC 20 0.0020791946 71.19047 3 ATATAAG 25 0.004951499 57.189587 1 CCTCGTC 25 0.005033655 56.952374 8 AAGCCTC 25 0.005033655 56.952374 5 TATAAGC 25 0.005033655 56.952374 2 TGGAACG 40 5.402859E-4 47.46031 5 ACGGAAA 40 5.402859E-4 47.46031 9 GAACGGA 40 5.402859E-4 47.46031 7 GTAAGCG 265 0.0 44.778103 94-95 CTCCGAC 50 6.4028427E-10 42.718307 94-95 CTGGAAC 45 9.6512836E-4 42.186947 4 CATGGGG 80 2.2686436E-7 41.527775 4 GGAACGG 50 0.0016202145 37.968246 6 GGAAAAG 50 0.0016202145 37.968246 6 CTGGTCG 55 0.0025866444 34.51659 9 TCTGGAA 55 0.0025866444 34.51659 3 ATGGGGT 70 2.0591765E-4 33.900223 5 AAGCAGG 350 0.0 32.547283 94-95 CCTGGTC 60 0.0039616986 31.640207 8 CCCTGGT 60 0.0039616986 31.640207 7 >>END_MODULE