##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938914_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 267866 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.929035413229002 31.0 31.0 34.0 30.0 34.0 2 32.11674120642411 33.0 31.0 34.0 30.0 34.0 3 32.22134948071051 34.0 31.0 34.0 30.0 34.0 4 35.73359440914487 37.0 35.0 37.0 35.0 37.0 5 35.663772184599765 37.0 35.0 37.0 33.0 37.0 6 35.80701544802252 37.0 35.0 37.0 35.0 37.0 7 35.788808583396175 37.0 35.0 37.0 33.0 37.0 8 35.825942822157344 37.0 35.0 37.0 33.0 37.0 9 37.3639954305511 39.0 37.0 39.0 34.0 39.0 10-11 37.250470384445954 39.0 37.0 39.0 33.5 39.0 12-13 37.26732022727782 39.0 37.0 39.0 33.5 39.0 14-15 38.16486974830699 40.0 37.0 41.0 33.0 41.0 16-17 38.28155308997782 40.0 37.5 41.0 33.5 41.0 18-19 38.50982020861177 40.0 38.0 41.0 34.0 41.0 20-21 38.46695549267171 40.0 38.0 41.0 34.0 41.0 22-23 38.419260003135896 40.0 38.0 41.0 34.0 41.0 24-25 38.215824330075485 40.0 37.5 41.0 33.5 41.0 26-27 37.95440630763143 39.5 37.0 41.0 33.0 41.0 28-29 37.89122359687306 40.0 37.0 41.0 33.0 41.0 30-31 37.95883202795427 40.0 37.0 41.0 33.0 41.0 32-33 37.716143519520955 40.0 37.0 41.0 33.0 41.0 34-35 37.5024881843907 39.5 36.5 41.0 32.5 41.0 36-37 37.34300172474297 39.5 36.0 41.0 32.0 41.0 38-39 37.16025176767488 39.0 36.0 41.0 31.0 41.0 40-41 37.37534252200727 40.0 36.0 41.0 32.0 41.0 42-43 37.12990450449105 40.0 36.0 41.0 31.0 41.0 44-45 36.922707622467954 39.0 36.0 41.0 31.0 41.0 46-47 36.68049696490036 39.0 36.0 41.0 30.5 41.0 48-49 36.32975069624365 39.0 35.0 41.0 30.0 41.0 50-51 36.0494743640477 39.0 35.0 40.0 29.0 41.0 52-53 36.11089873294856 39.0 35.0 40.0 28.5 41.0 54-55 36.02970141787311 39.0 35.0 40.5 28.5 41.0 56-57 35.51550962048188 38.5 34.5 40.0 27.5 41.0 58-59 35.22113108793202 38.0 34.0 40.0 27.0 41.0 60-61 34.376163828182754 38.0 33.0 40.0 26.0 41.0 62-63 34.20318928120777 37.0 33.5 40.0 26.0 41.0 64-65 34.02082197815325 37.0 33.5 39.5 26.0 40.5 66-67 33.25198046784586 36.0 32.5 39.0 23.5 40.0 68-69 32.631942837090186 36.0 32.0 38.0 22.5 39.0 70-71 31.774993840203685 35.0 31.5 37.0 21.5 38.5 72-73 31.106316217810395 34.5 30.5 36.5 20.0 37.5 74-75 30.77535036174804 34.0 30.5 36.0 19.0 37.0 76-77 28.860794203071684 32.0 27.5 34.0 15.0 35.5 78-79 29.87854188288174 34.0 29.5 35.0 15.0 36.0 80-81 29.731152889877777 34.0 29.5 35.0 12.0 36.0 82-83 29.368869136060567 34.0 29.5 35.0 9.0 35.5 84-85 28.927626126496087 33.5 29.0 35.0 4.5 35.0 86-87 28.73094009691413 34.0 29.0 35.0 2.0 35.0 88-89 28.0919993578879 33.0 28.0 35.0 2.0 35.0 90-91 27.533621288256068 33.0 26.0 35.0 2.0 35.0 92-93 27.249727475678135 33.0 26.0 34.5 2.0 35.0 94-95 26.75114609543578 32.0 25.0 34.0 2.0 35.0 96-97 25.95991092561206 32.0 25.0 34.0 2.0 35.0 98-99 25.168879215727266 32.0 21.0 34.0 2.0 35.0 100-101 20.651400327029187 27.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19156949534367484 1101 2 0.29308024857541426 1101 3 0.027006356575476786 1101 4 0.05989708811409855 1101 5 0.16026154303357032 1101 6 0.18232856870105252 1101 7 0.2087234538435041 1101 8 0.3936337788732942 1101 9 0.17357838903731704 1101 10-11 0.4681054099159567 1101 12-13 0.3530023351564182 1101 14-15 0.3931844543574101 1101 16-17 0.5420336805009285 1101 18-19 0.4532158854298416 1101 20-21 0.43271386265058 1101 22-23 0.3276589167987112 1101 24-25 0.3415891601274481 1101 26-27 0.49936196981745695 1101 28-29 0.24553731983034055 1101 30-31 0.5467642750228663 1101 32-33 0.5800447216397018 1101 34-35 0.6886809102791389 1101 36-37 0.6135689614055835 1101 38-39 0.5461137766966928 1101 40-41 0.7234982706958846 1101 42-43 0.6398226213397322 1101 44-45 0.8783376106770504 1101 46-47 0.8981649797765172 1101 48-49 0.8275304447312024 1101 50-51 0.6505380784849919 1101 52-53 0.628803928464194 1101 54-55 0.4423741608602043 1101 56-57 0.4724839040380573 1101 58-59 0.5591310772087326 1101 60-61 0.7215480421786324 1101 62-63 0.6081733156841764 1101 64-65 0.2977324780664361 1101 66-67 0.34694226020737773 1101 68-69 0.8337601321987691 1101 70-71 0.9364250524050597 1101 72-73 0.9342566995703017 1101 74-75 0.8694875010595453 1101 76-77 0.373417550975752 1101 78-79 0.6230851715819945 1101 80-81 1.1352725171857223 1101 82-83 0.8798858081952083 1101 84-85 1.037961287927974 1101 86-87 1.2212691338508996 1101 88-89 0.6326198996085566 1101 90-91 0.3742545266425772 1101 92-93 0.5142342256334409 1101 94-95 0.5611864574575272 1101 96-97 0.3389416573066022 1101 98-99 0.6444237089313916 1101 100-101 1.2483105925539348 1102 1 0.44683662243742717 1102 2 0.4683600771060128 1102 3 0.31337612715577023 1102 4 0.1291199189468344 1102 5 0.23200878444025363 1102 6 0.2139551464576641 1102 7 0.23984197412610797 1102 8 0.33592162518591095 1102 9 0.5255669577441537 1102 10-11 0.3921746327467943 1102 12-13 0.32749626228191886 1102 14-15 0.5532622506581149 1102 16-17 0.6411683951155212 1102 18-19 0.2718155520171237 1102 20-21 0.5434148231967413 1102 22-23 0.40567749265010633 1102 24-25 0.479780793055923 1102 26-27 0.12542373055414657 1102 28-29 0.573536049686659 1102 30-31 0.41285937703128894 1102 32-33 0.5179585106370936 1102 34-35 0.5799883644348895 1102 36-37 0.6746073038680649 1102 38-39 0.8145824597164619 1102 40-41 0.7253796710293017 1102 42-43 0.5121414274046643 1102 44-45 0.5497673161624803 1102 46-47 0.8760089108712279 1102 48-49 0.7144082727855121 1102 50-51 0.5562616634655413 1102 52-53 0.9097279579950381 1102 54-55 1.176779177927763 1102 56-57 1.2660299864386317 1102 58-59 0.9358160021908688 1102 60-61 0.9799746516888277 1102 62-63 1.2324806904559509 1102 64-65 1.1856661130588932 1102 66-67 1.0738057991452266 1102 68-69 1.0977039283407066 1102 70-71 0.6907872021232428 1102 72-73 0.9640971377698726 1102 74-75 1.3874203422122058 1102 76-77 1.2800858053220239 1102 78-79 1.3647617612462035 1102 80-81 1.139265531395452 1102 82-83 1.0497992796566677 1102 84-85 1.2034213180938842 1102 86-87 1.5323987678907507 1102 88-89 2.043011262631495 1102 90-91 2.2100487633656094 1102 92-93 2.053846831809512 1102 94-95 2.357598301547391 1102 96-97 1.978871799403926 1102 98-99 2.3269830484566754 1102 100-101 2.824598597634509 1103 1 0.23610390829913896 1103 2 0.3052259975632694 1103 3 0.2942128343082686 1103 4 0.3635408128104558 1103 5 0.4760510167177827 1103 6 0.23496014764842243 1103 7 0.4799785145722453 1103 8 0.5393827667275488 1103 9 0.3395702918713255 1103 10-11 0.4316681629523984 1103 12-13 0.4521926185572269 1103 14-15 0.6522937660983033 1103 16-17 0.6311025064118638 1103 18-19 0.6232563712597994 1103 20-21 0.7485033363347853 1103 22-23 0.6049868520213835 1103 24-25 0.4650709415039671 1103 26-27 0.7807384880360857 1103 28-29 0.6281284129477527 1103 30-31 0.639881683929751 1103 32-33 0.7460366244737173 1103 34-35 0.5672234204005946 1103 36-37 0.7228807022962656 1103 38-39 0.8679761248748363 1103 40-41 0.8631743840562152 1103 42-43 0.907029099072524 1103 44-45 0.8074874082479013 1103 46-47 1.2099058704647803 1103 48-49 1.0461539265125808 1103 50-51 1.4157202647198162 1103 52-53 1.446617693646381 1103 54-55 1.4990543228035236 1103 56-57 1.000823985009724 1103 58-59 0.7959731824718901 1103 60-61 1.1324792162677042 1103 62-63 1.2053393075870105 1103 64-65 0.9981373363660282 1103 66-67 0.8813552156729685 1103 68-69 0.9876062860449153 1103 70-71 0.9424979268989908 1103 72-73 1.256119047748438 1103 74-75 0.5617951933672352 1103 76-77 0.8713932594777738 1103 78-79 1.1190365885860452 1103 80-81 0.8599688734610247 1103 82-83 1.1167279134583659 1103 84-85 1.3213620976445704 1103 86-87 1.0066942350654777 1103 88-89 1.389704919851468 1103 90-91 1.1536067533632277 1103 92-93 1.358363780289313 1103 94-95 1.198838279319876 1103 96-97 1.632463924513079 1103 98-99 1.8185127777573022 1103 100-101 2.078270106723977 1104 1 -0.3113490614746901 1104 2 -0.11987521701163217 1104 3 0.0491190990433239 1104 4 0.14589289319678045 1104 5 0.1575462283334801 1104 6 0.18640967043592838 1104 7 0.04801584608453169 1104 8 0.05857695237703098 1104 9 0.177350562752423 1104 10-11 0.07667734949614413 1104 12-13 -0.2905760268747031 1104 14-15 0.2077626050167254 1104 16-17 0.11159126305788192 1104 18-19 -0.020435806357554043 1104 20-21 0.36115637764984143 1104 22-23 0.10666969676066174 1104 24-25 0.05769243964226689 1104 26-27 -0.10892543079714301 1104 28-29 0.111641412313638 1104 30-31 -0.10197643681232904 1104 32-33 -0.12109180828566224 1104 34-35 0.05643532876278101 1104 36-37 -0.04500053926918923 1104 38-39 0.25089240538147095 1104 40-41 0.06383939226719093 1104 42-43 0.03082793815354279 1104 44-45 -0.04413771572979641 1104 46-47 0.03757281354072717 1104 48-49 0.07266482841981059 1104 50-51 -0.38966321250894254 1104 52-53 -0.5803972487141564 1104 54-55 -0.3831546751315358 1104 56-57 -0.23448973952404373 1104 58-59 -0.30677790284102 1104 60-61 0.005582967304235353 1104 62-63 -0.12957931426883107 1104 64-65 -0.2676057981152127 1104 66-67 0.08273563614026358 1104 68-69 0.20505096354033014 1104 70-71 0.07082500037508055 1104 72-73 0.03577207280412509 1104 74-75 -0.16256784583410067 1104 76-77 -0.29154188189744445 1104 78-79 -0.025883171425661544 1104 80-81 -0.4284642111039574 1104 82-83 0.19447208853925702 1104 84-85 0.1239977414343052 1104 86-87 -0.19455611044612553 1104 88-89 -0.3059847213042488 1104 90-91 -0.3449949408630708 1104 92-93 -0.3521300167612402 1104 94-95 -0.42969203627448493 1104 96-97 -0.8466183966745078 1104 98-99 -0.46897726337890333 1104 100-101 -0.8054510125710195 1105 1 -0.25512416187629583 1105 2 -0.28453385154978506 1105 3 -0.44084074031410836 1105 4 -0.1392476690522173 1105 5 -0.0994818841163223 1105 6 -0.36379113277692454 1105 7 -0.1768837523090454 1105 8 -0.218531481357914 1105 9 -0.16401489909496547 1105 10-11 -0.23055156616650407 1105 12-13 -0.2584475128184778 1105 14-15 -0.3504703267101803 1105 16-17 -0.3281677730866974 1105 18-19 -0.31360849712060457 1105 20-21 -0.19707655407706426 1105 22-23 -0.4174266887815037 1105 24-25 -0.6089742270243974 1105 26-27 -0.11896559143971075 1105 28-29 -0.3682782664012265 1105 30-31 -0.4694463163303979 1105 32-33 -0.07490706932983215 1105 34-35 -0.4736851531649222 1105 36-37 -0.31407684449006723 1105 38-39 -0.1366577954217334 1105 40-41 -0.11487546717055608 1105 42-43 -0.24427246345288012 1105 44-45 -0.060201972757901956 1105 46-47 -0.092949274812689 1105 48-49 -0.33496569366854345 1105 50-51 0.3593327714268 1105 52-53 0.28707263080391954 1105 54-55 0.06463648952709633 1105 56-57 -0.0758552013714322 1105 58-59 -0.047741758262709766 1105 60-61 0.2666271440110606 1105 62-63 0.20375401906449753 1105 64-65 0.3950448042944217 1105 66-67 0.7313300136503003 1105 68-69 0.7010348992833002 1105 70-71 0.7736362453549965 1105 72-73 0.2506315105551309 1105 74-75 0.5944602666156946 1105 76-77 0.11006454380536468 1105 78-79 0.5765264671285557 1105 80-81 0.6217365921088955 1105 82-83 0.7527050202661663 1105 84-85 0.3107447293861121 1105 86-87 0.7713073433691378 1105 88-89 1.382770298776073 1105 90-91 1.1068122880525912 1105 92-93 1.234215364764868 1105 94-95 0.7048461163359576 1105 96-97 1.1272771856548118 1105 98-99 1.3643560699544324 1105 100-101 0.9515770998787829 1106 1 -0.21475957854296368 1106 2 -0.4519064670116322 1106 3 -0.1144884561775612 1106 4 0.004656070907621768 1106 5 -0.017638887128370584 1106 6 -0.16504301530704168 1106 7 -0.1314049621484017 1106 8 0.1087463800878723 1106 9 -0.13803598343231727 1106 10-11 9.368876487556577E-4 1106 12-13 0.12984001228192454 1106 14-15 -0.09808578855756878 1106 16-17 0.0847652088410129 1106 18-19 -0.020325992100524104 1106 20-21 -0.058224269940517104 1106 22-23 0.1777822720618687 1106 24-25 0.19957245972258875 1106 26-27 0.05273684329924322 1106 28-29 0.05596558400038276 1106 30-31 0.15549877654108712 1106 32-33 0.14401549593120677 1106 34-35 0.1772310114937099 1106 36-37 -0.02481824760251783 1106 38-39 0.12699054795175613 1106 40-41 0.03542103132341623 1106 42-43 0.2516251896595705 1106 44-45 0.24695634802522903 1106 46-47 0.3208465334202444 1106 48-49 0.08113621396197601 1106 50-51 0.3759460997400552 1106 52-53 0.2707573697597425 1106 54-55 0.4960653298885447 1106 56-57 0.6788746555562781 1106 58-59 0.9077599360144006 1106 60-61 0.7616305953162836 1106 62-63 0.6564083865343804 1106 64-65 0.6064764684510493 1106 66-67 0.3457956888511049 1106 68-69 0.8289998842230588 1106 70-71 0.8039841996722679 1106 72-73 0.8463581671816378 1106 74-75 0.9840655628004349 1106 76-77 0.7812806582631993 1106 78-79 0.9183233421285557 1106 80-81 0.9402842201209438 1106 82-83 1.0849402110292168 1106 84-85 1.093249749466434 1106 86-87 1.0584480938711458 1106 88-89 1.3876727209648294 1106 90-91 1.3745708221891384 1106 92-93 1.6831360474957897 1106 94-95 1.7007202378219013 1106 96-97 1.234761811658828 1106 98-99 1.1197526562998128 1106 100-101 2.156553254497254 1107 1 0.20999211719346889 1107 2 0.20183020934495488 1107 3 0.12500403413251604 1107 4 0.14234381315568356 1107 5 0.05088703341426282 1107 6 0.20980557965420132 1107 7 -0.028055882691035094 1107 8 0.05890384132818127 1107 9 0.08895045067620799 1107 10-11 0.25275374811386797 1107 12-13 0.20424044832843435 1107 14-15 0.0426603354734425 1107 16-17 0.21164263132163086 1107 18-19 0.295595761775445 1107 20-21 0.1052023288966879 1107 22-23 0.40946226237194594 1107 24-25 0.110788544993909 1107 26-27 -0.07544266251471043 1107 28-29 0.18208598128720865 1107 30-31 0.5614913879751953 1107 32-33 0.6102521722877938 1107 34-35 0.4275943835867295 1107 36-37 0.24569713514942038 1107 38-39 0.3802003347734626 1107 40-41 0.4331772086489991 1107 42-43 0.4866512313262419 1107 44-45 0.458454046668642 1107 46-47 0.47149454698613624 1107 48-49 0.05218757055111922 1107 50-51 0.22687269857726733 1107 52-53 0.6801565945659505 1107 54-55 0.5593829170978424 1107 56-57 0.7682187689283708 1107 58-59 0.8290444563632349 1107 60-61 0.8338962203162836 1107 62-63 0.8820018942863186 1107 64-65 1.1127294965518217 1107 66-67 1.086938494083661 1107 68-69 0.7959027427501937 1107 70-71 0.932784466145133 1107 72-73 0.6911377214452052 1107 74-75 0.8475890608624503 1107 76-77 0.3088363753174619 1107 78-79 0.6346660020122776 1107 80-81 0.9616777520976889 1107 82-83 0.5749474784710777 1107 84-85 0.5130656409393026 1107 86-87 0.5924990870881999 1107 88-89 0.6213058362749067 1107 90-91 1.7133547369178181 1107 92-93 1.4522736443950173 1107 94-95 1.4991395692172524 1107 96-97 1.3413660994495267 1107 98-99 1.1093359896331485 1107 100-101 1.1286341653499647 1108 1 -0.015908475601786876 1108 2 0.005614979066798753 1108 3 0.1839643624498919 1108 4 0.019315997378207328 1108 5 0.20455780404810042 1108 6 0.03356298959491966 1108 7 0.07121452314571286 1108 8 -0.07192151206898956 1108 9 -0.007766375589177699 1108 10-11 -0.1372371319590897 1108 12-13 -0.1607390318357247 1108 14-15 -0.1506593179693283 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-2.6499743891987926 2316 66-67 -2.796782436148895 2316 68-69 -2.5296480850198435 2316 70-71 -2.5878792171258382 2316 72-73 -2.3442717618404423 2316 74-75 -2.5543684868841083 2316 76-77 -2.415609128803048 2316 78-79 -2.584356813628542 2316 80-81 -2.5586252337434097 2316 82-83 -3.89576266751968 2316 84-85 -3.830153247378991 2316 86-87 -3.4819390808133974 2316 88-89 -3.5643494103443203 2316 90-91 -3.262209540586884 2316 92-93 -3.6178300408322848 2316 94-95 -4.093406832367375 2316 96-97 -4.5259033598711405 2316 98-99 -4.779202391018799 2316 100-101 -5.207627542032714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 13.0 6 46.0 7 75.0 8 93.0 9 173.0 10 270.0 11 415.0 12 598.0 13 845.0 14 957.0 15 1251.0 16 1663.0 17 2123.0 18 2121.0 19 1758.0 20 1800.0 21 1929.0 22 2042.0 23 2374.0 24 2718.0 25 3145.0 26 3575.0 27 4163.0 28 5219.0 29 6281.0 30 7530.0 31 9137.0 32 11299.0 33 14263.0 34 19343.0 35 28404.0 36 43037.0 37 59244.0 38 29009.0 39 951.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.63893979082116 20.30818465597164 15.77197932944355 14.280896223763644 2 13.970418044843317 29.38073514369125 28.761022302195876 27.88782450926956 3 32.87240635242995 22.774446924955015 32.32773102969395 12.025415692921088 4 11.539351765434956 17.464329179515133 43.67370252290324 27.32261653214667 5 8.768628856434608 43.74272018636323 35.0592091509124 12.42944180628976 6 23.97318062016083 38.70032030940844 12.989703807127444 24.336795263303294 7 19.81849133521985 35.86532072006152 23.889556718657836 20.426631226060792 8 23.08393002471385 21.024318129213864 20.051816953252747 35.83993489281954 9 15.199390740146193 27.36032195202079 30.90388477821 26.53640252962302 10-11 26.40611350451345 22.5736002329523 25.564461335145182 25.45582492738907 12-13 34.384543017777546 22.8608707338744 23.940888354625073 18.81369789372298 14-15 23.65802348610564 28.197886586488114 32.984146598695965 15.159943328710279 16-17 17.106319275151847 30.237098815439232 31.56856303408832 21.088018875320593 18-19 16.75520596118955 37.179970582306076 26.77402133902772 19.290802117476648 20-21 22.723675270471055 26.55973509142631 22.688769758013336 28.0278198800893 22-23 18.07172242837837 27.22331314911187 28.916510494053 25.788453928456768 24-25 28.407300665258 27.845265916540356 23.584926791754086 20.162506626447552 26-27 23.435411735718606 21.379533050107145 36.891206797428566 18.293848416745686 28-29 16.783018374859072 37.43550879917571 26.70869016597851 19.072782659986707 30-31 20.20674516362659 26.308490065928485 25.787147304995784 27.69761746544914 32-33 21.759013835275848 27.941769392158765 30.436486900166503 19.862729872398884 34-35 27.53638067363045 24.91581485989054 26.817727038549698 20.73007742792931 36-37 21.464891583537423 23.615487426079685 35.841086255301356 19.078534735081536 38-39 20.221960872162484 29.288754480286737 31.124551971326163 19.364732676224612 40-41 19.66942303025778 31.30886827319732 28.228211972448808 20.793496724096094 42-43 18.367053614593893 30.995779598343198 22.589695201509713 28.047471585553197 44-45 22.649197733194956 22.93945480202788 26.477417813384303 27.93392965139286 46-47 27.812227009026902 30.04431320137681 27.892864342619074 14.250595446977222 48-49 23.14333126139798 26.610556417306448 29.604409675751448 20.641702645544125 50-51 12.336205416140906 29.68461842861729 37.545638490887235 20.433537664354564 52-53 20.3642866209224 33.9104253619347 23.158780882978803 22.566507134164095 54-55 16.813817356439415 25.16482121657844 28.972321981886466 29.04903944509568 56-57 24.594573406106036 23.22952521036638 26.797353900831013 25.378547482696572 58-59 30.957571911223937 23.489444309633583 25.838575401788216 19.714408377354264 60-61 21.571570170182504 28.598948329254704 33.605304273322396 16.2241772272404 62-63 16.914614023429625 29.17802184674427 32.09589869561646 21.811465434209644 64-65 16.44534954044186 36.31367922767354 26.696743894335228 20.544227337549373 66-67 21.740777934989154 26.70133613078327 22.543240387810187 29.014645546417384 68-69 17.485925693635576 27.013895110951825 28.59958785055103 26.900591344861574 70-71 26.814899987307083 27.396907408928346 23.731081958889895 22.057110644874676 72-73 22.406539090440745 21.461663667654722 35.455040953312476 20.676756288592056 74-75 16.478201787460893 35.96686402902944 26.081510904706086 21.47342327880358 76-77 19.76086846831358 25.954255535850105 25.7795228208401 28.505353174996216 78-79 21.023011505752876 27.04635900039572 29.447186279707015 22.483443214144387 80-81 25.81981289152039 24.878110697139615 26.128922670290365 23.173153741049628 82-83 20.53508097332248 23.775880477552207 33.920878349622576 21.768160199502738 84-85 19.69884195829258 28.186854621340522 29.82629374388687 22.28800967648003 86-87 19.256233877901977 29.89831395566189 27.521776515009904 23.32367565142622 88-89 18.18928143678904 29.56292418508668 22.88017573750335 29.36761864062093 90-91 21.898108183515376 22.723331903969203 26.331508865490594 29.04705104702483 92-93 25.391613717306416 28.60908065973285 27.401573921289003 18.597731701671734 94-95 22.104149089470106 25.44555860019562 28.81851373447918 23.63177857585509 96-97 13.851027211874817 28.031329682185913 34.70270028111595 23.414942824823324 98-99 20.053870218691436 30.8223141421457 23.78801340968992 25.335802229472947 100-101 17.873675643792044 25.009333024721315 27.69985739138226 29.417133940104378 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2546.0 1 1502.0 2 390.5 3 277.5 4 208.5 5 233.0 6 348.0 7 641.0 8 849.0 9 742.5 10 519.0 11 334.5 12 284.5 13 261.5 14 235.5 15 218.5 16 210.5 17 226.0 18 245.0 19 249.5 20 258.5 21 285.0 22 319.0 23 361.5 24 402.0 25 477.0 26 699.0 27 939.5 28 1015.5 29 1143.0 30 1439.0 31 1757.5 32 2184.0 33 2769.5 34 3411.5 35 3853.0 36 4304.0 37 4776.5 38 4692.5 39 4345.0 40 3864.0 41 3260.5 42 2817.5 43 2724.0 44 2744.0 45 3310.0 46 7181.0 47 27830.0 48 62258.5 49 59977.5 50 27907.5 51 10212.5 52 3287.0 53 803.5 54 266.5 55 164.5 56 98.0 57 60.0 58 50.0 59 51.0 60 56.5 61 52.5 62 47.0 63 37.5 64 22.5 65 18.0 66 14.5 67 18.5 68 17.5 69 9.0 70 4.5 71 5.5 72 4.0 73 2.5 74 2.0 75 1.0 76 1.5 77 1.0 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3781741617077195 2 0.0 3 0.0 4 0.0 5 7.466419777052705E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0020532654386894943 16-17 0.001119962966557906 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.001493283955410541 36-37 0.0037332098885263522 38-39 0.009706345710168518 40-41 3.7332098885263526E-4 42-43 1.8666049442631763E-4 44-45 0.0 46-47 0.0 48-49 1.8666049442631763E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.7332098885263526E-4 60-61 0.0024265864275421294 62-63 0.0 64-65 0.0 66-67 0.001119962966557906 68-69 7.466419777052705E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.11740945099415379 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14036869180859085 88-89 0.4123330321877357 90-91 0.3163895380526084 92-93 0.0 94-95 0.0 96-97 0.0018666049442631761 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 267866.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.752644979206021 #Duplication Level Percentage of deduplicated Percentage of total 1 67.93325526932085 8.663286867314254 2 11.40807962529274 2.9096637871174393 3 5.234192037470726 2.0024937842055355 4 3.185011709601874 1.6246929434866688 5 2.177985948477752 1.388754078531803 6 1.6334894613583137 1.249878670678623 7 1.1973067915690867 1.0688179910850948 8 0.9923887587822015 1.012446521768347 9 0.7728337236533959 0.8870106695138614 >10 4.885831381733021 11.536365197524136 >50 0.2751756440281031 2.3362427482397914 >100 0.16978922716627637 3.899711049554628 >500 0.043911007025761124 3.779875012132932 >1k 0.05562060889929743 17.01111749904803 >5k 0.026346604215456676 22.203265812010482 >10k+ 0.008782201405152224 18.426377367788373 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24320 9.079166448896089 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14960 5.584881993235424 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10078 3.7623289256568584 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7756 2.895477589541039 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7358 2.7468958359776905 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7237 2.7017239963265216 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7044 2.6296730454779627 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6942 2.5915943046149943 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6549 2.4448791559959084 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5941 2.2178999947735063 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5583 2.084251080764263 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5065 1.8908708085385977 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4673 1.7445289809083646 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4169 1.5563752025266364 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4147 1.5481621407718784 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3768 1.4066734859967298 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3304 1.233452547169107 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2901 1.083004188661495 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2593 0.9680213240948832 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2482 0.9265826943322407 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2243 0.837358977996461 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2169 0.8097332248213659 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2077 0.7753876938469234 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1941 0.724616039362965 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1571 0.58648727348749 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1502 0.5607281252566582 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1403 0.5237693473602473 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1291 0.48195739660875214 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1146 0.42782585322512007 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1097 0.40953312477134085 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1090 0.4069198778493725 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 987 0.368467815997551 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 939 0.35054840853262453 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 931 0.3475618406218034 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 824 0.30761649481457143 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 760 0.2837239515280028 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 671 0.2504983835201183 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 618 0.2307123711109286 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 598 0.2232459513338759 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 576 0.2150328895791179 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 569 0.21241964265714947 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 560 0.20905975375747576 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 541 0.20196665496927568 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 527 0.1967401611253388 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 523 0.19524687716992825 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 501 0.18703381541517028 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 426 0.1590347412512226 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 400 0.1493283955410541 TruSeq Adapter, Index 3 (95% over 21bp) CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 379 0.14148865477514877 No Hit CCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 351 0.131035667087275 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 348 0.12991570412071707 No Hit TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 315 0.11759611148858011 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 290 0.10826308676726423 No Hit GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT 273 0.10191662995676944 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.7332098885263526E-4 0.0 10-11 0.0 0.0 0.0 3.7332098885263526E-4 0.0 12-13 0.0 0.0 0.0 9.333024721315882E-4 0.0 14-15 0.0 0.0 0.0 0.001493283955410541 0.0 16-17 0.0 0.0 0.0 0.0031732284052474 0.0 18-19 0.0 0.0 0.0 0.004479851866231623 0.0 20-21 0.0 0.0 0.0 0.005973135821642165 0.0 22-23 0.0 0.0 0.0 0.008773043238036928 0.0 24-25 0.0 0.0 0.0 0.014372858070826458 0.0 26-27 0.0 0.0 0.0 0.026692450702963423 0.0 28-29 0.0 0.0 0.0 0.06831774096003226 0.0 30-31 0.0 0.0 0.0 0.16612784003942271 0.0 32-33 0.0 0.0 0.0 0.3428953282611455 0.0 34-35 0.0 0.0 0.0 0.5336623535648422 0.0 36-37 0.0 0.0 0.0 0.7570949653931442 0.0 38-39 0.0 0.0 0.0 1.0760977503677212 0.0 40-41 0.0 0.0 0.0 1.4059268440190245 0.0 42-43 0.0 0.0 0.0 1.7475155488191856 0.0 44-45 0.0 0.0 0.0 2.1378226426646156 0.0 46-47 0.0 0.0 0.0 2.6057805021913945 0.0 48-49 0.0 0.0 0.0 2.9425160341364713 0.0 50-51 0.0 0.0 0.0 3.320316874855338 0.0 52-53 0.0 0.0 0.0 3.679824987120426 0.0 54-55 0.0 0.0 0.0 4.107464179851119 0.0 56-57 0.0 0.0 0.0 4.746963033755684 0.0 58-59 0.0 0.0 0.0 5.206147850044426 0.0 60-61 0.0 0.0 0.0 5.752129796241404 0.0 62-63 0.0 0.0 0.0 6.307818088148553 0.0 64-65 0.0 0.0 0.0 6.861079793628157 0.0 66-67 0.0 0.0 0.0 7.580469339147186 0.0 68-69 0.0 0.0 0.0 8.433134477686604 0.0 70-71 0.0 0.0 0.0 9.245667609924364 0.0 72-73 0.0 0.0 0.0 9.899166000910903 0.0 74-75 0.0 0.0 0.0 10.830415207603803 0.0 76-77 0.0 0.0 0.0 11.539351765434956 0.0 78-79 0.0 0.0 0.0 12.814616263355557 0.0 80-81 0.0 0.0 0.0 13.983297618958733 0.0 82-83 0.0 0.0 0.0 14.877401387260793 0.0 84-85 0.0 0.0 0.0 15.97459177349869 0.0 86-87 0.0 0.0 0.0 16.983118424884083 0.0 88-89 0.0 0.0 0.0 18.173265737346284 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACAGC 15 6.6352665E-4 94.9173 9 GGGTTGC 35 2.7960748E-4 54.238457 6 GGTTGCA 35 2.7960748E-4 54.238457 7 GTGTAGG 40 5.324752E-4 47.600826 1 TTGCAAG 40 5.403887E-4 47.458656 9 GTTGCAA 40 5.403887E-4 47.458656 8 GTAAGCG 135 0.0 42.18547 94-95 CTCCGAC 30 8.3274426E-5 39.548874 94-95 GTACCCA 50 0.0015968741 38.080658 1 CGTGTGT 25 0.0016034585 38.05572 80-81 TCGTGTG 25 0.0016219699 37.966923 78-79 AATTGCG 25 0.0016219699 37.966923 42-43 GAATTGC 25 0.0016219699 37.966923 42-43 ATGGGAT 55 0.0025871343 34.515385 5 GTAGGGT 55 0.0025871343 34.515385 3 AGTACTG 55 0.0025871343 34.515385 8 GCTTTGC 595 0.0 32.000553 1 ACCGGTA 30 0.003962333 31.645008 34-35 GACACCG 30 0.003962333 31.645008 30-31 CCGGTAG 30 0.003962333 31.645008 34-35 >>END_MODULE