FastQCFastQC Report
Thu 26 May 2016
SRR938913_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938913_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122676
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT146170.688611921932504No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100880.475249166617986No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA91970.4332738486702634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80650.379944937428039No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT77820.3666127096174828No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA75950.35780307498647934No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT59500.2803065564410207No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC51910.24454980411518293No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT51850.24426714204146088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51540.24280672132723033No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41850.19715679642112127No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT34750.16370845103068013No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34390.1620124785883479No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA32980.15536991985588003No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC32910.15504014743653766No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG31120.14660739557049685No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG30620.14425187828947988No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT30220.1423674644646663No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA29920.1409541540960561No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC27730.13063698840520171No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC24780.11673943644720156No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG23690.11160440877458454No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA23030.10849512596364211No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT6750.024.5589943
GTACATG109300.022.6222231
TACATGG107700.022.5604612
GAGTACT79900.021.83523612-13
ACATGGG111450.020.8664113
GTGTACG2056.891896E-720.8356191
GTACTTT83800.020.48126214-15
CATGGGG41900.020.4602474
CTTTGCG8200.019.638642
GTAAGCG2800.019.53544494-95
AGTACTT85050.019.0657312-13
CATGGGA65550.018.2807564
GTATAGG6700.017.7085481
CTATACG1901.9080561E-417.4498124
ACTTTTT100800.017.31074516-17
ATGGGAT26900.016.7269845
AGTACAT83050.016.3107052
GTCTAGA6750.016.1711831
TACTTTT98900.016.06160514-15
TACCTGG12600.015.4120242
Produced by FastQC (version 0.11.5)