##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938913_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2122676 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8578501853321 33.0 31.0 34.0 30.0 34.0 2 31.927145263808512 33.0 31.0 34.0 30.0 34.0 3 31.972807437404484 34.0 31.0 34.0 30.0 34.0 4 35.51031057024247 37.0 35.0 37.0 33.0 37.0 5 35.559010890027494 37.0 35.0 37.0 33.0 37.0 6 35.66166386203076 37.0 35.0 37.0 33.0 37.0 7 35.589160097914146 37.0 35.0 37.0 33.0 37.0 8 35.58199461434529 37.0 35.0 37.0 33.0 37.0 9 37.185374498981474 39.0 37.0 39.0 34.0 39.0 10-11 37.074388649044884 39.0 37.0 39.0 33.0 39.0 12-13 36.97210078222018 39.0 37.0 39.0 33.0 39.0 14-15 38.136613642402324 40.0 38.0 41.0 33.0 41.0 16-17 38.06315966261455 40.0 38.0 41.0 33.0 41.0 18-19 38.01832309782557 40.0 38.0 41.0 33.0 41.0 20-21 38.136567709815345 40.0 38.0 41.0 33.0 41.0 22-23 38.08115393022769 40.0 38.0 41.0 33.0 41.0 24-25 37.98591965990099 40.0 38.0 41.0 33.0 41.0 26-27 37.84678796952526 40.0 38.0 41.0 32.5 41.0 28-29 37.72470150885015 40.0 37.5 41.0 32.0 41.0 30-31 37.536380022198394 40.0 37.0 41.0 32.0 41.0 32-33 37.317521373963814 40.0 36.5 41.0 31.5 41.0 34-35 36.98672878008702 40.0 36.0 41.0 30.5 41.0 36-37 36.70415362495265 39.5 36.0 41.0 30.0 41.0 38-39 36.44251407186024 39.0 35.5 41.0 30.0 41.0 40-41 36.299447489866566 39.0 35.0 41.0 30.0 41.0 42-43 36.09605422589222 39.0 35.0 41.0 29.5 41.0 44-45 36.00152566854291 39.0 35.0 41.0 29.0 41.0 46-47 35.74856030783784 39.0 35.0 41.0 27.5 41.0 48-49 35.47428811556733 39.0 35.0 41.0 26.5 41.0 50-51 34.34957737308944 38.0 34.0 40.0 24.5 40.5 52-53 34.4499864793308 38.0 34.0 39.5 24.5 40.5 54-55 34.95095247696775 39.0 35.0 40.0 25.5 41.0 56-57 34.85620438540785 38.5 34.5 40.0 25.0 41.0 58-59 34.64768975576112 38.0 34.0 40.0 25.0 41.0 60-61 34.30539729096669 38.0 34.0 40.0 23.0 41.0 62-63 33.92478715545849 37.5 34.0 40.0 22.5 41.0 64-65 33.51184990078561 37.0 33.0 40.0 22.0 41.0 66-67 33.069974645211985 36.0 33.0 39.0 20.5 41.0 68-69 32.62637138216101 36.0 32.0 39.0 19.5 40.5 70-71 32.14378360145402 35.0 32.0 38.5 18.0 40.0 72-73 31.687627551260768 35.0 31.0 37.5 14.5 40.0 74-75 31.217539087453762 35.0 31.0 37.0 10.0 39.0 76-77 30.774940452523136 35.0 31.0 36.5 7.5 39.0 78-79 30.35712068163017 34.5 30.0 36.0 4.5 38.5 80-81 29.905746802620843 34.0 30.0 35.5 2.0 37.0 82-83 29.42986164633698 34.0 29.0 35.0 2.0 37.0 84-85 29.004660155388763 34.0 29.0 35.0 2.0 36.5 86-87 28.564325172565198 34.0 29.0 35.0 2.0 36.0 88-89 28.053018925168043 34.0 27.5 35.0 2.0 35.5 90-91 27.565697261381388 33.5 26.5 35.0 2.0 35.0 92-93 27.062789375297974 33.0 25.5 35.0 2.0 35.0 94-95 26.51184754526833 33.0 25.0 35.0 2.0 35.0 96-97 25.818266659631522 33.0 23.5 35.0 2.0 35.0 98-99 24.938389325549448 32.0 10.5 35.0 2.0 35.0 100-101 21.658371319975352 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17589551305177764 1101 2 0.24181512940835503 1101 3 0.27452661872692374 1101 4 0.2517256419858356 1101 5 0.15542933389411218 1101 6 0.2932367584141673 1101 7 0.32469585481316443 1101 8 0.2891571392446295 1101 9 0.3396792768662209 1101 10-11 0.2593335653401567 1101 12-13 0.2717352025893973 1101 14-15 0.3638658628121547 1101 16-17 0.28536348524768584 1101 18-19 0.5155001307391203 1101 20-21 0.5027659786811824 1101 22-23 0.5110623088417157 1101 24-25 0.6158240763439267 1101 26-27 0.5674076124956642 1101 28-29 0.5398647887639569 1101 30-31 0.3838426916643627 1101 32-33 0.35637358643356976 1101 34-35 0.322576387755646 1101 36-37 0.2133291577448162 1101 38-39 0.4177760820161325 1101 40-41 0.28146381470237003 1101 42-43 0.3787481235174468 1101 44-45 0.43299127108399915 1101 46-47 0.5227714014594582 1101 48-49 0.3457703068358384 1101 50-51 0.32536894361714275 1101 52-53 0.3963046989781773 1101 54-55 0.5862139411406062 1101 56-57 0.49207904595341034 1101 58-59 0.36337683779301244 1101 60-61 0.47420982333488126 1101 62-63 0.2683518431093148 1101 64-65 0.319820355815267 1101 66-67 0.34716432935431385 1101 68-69 0.3228550191351829 1101 70-71 0.19400684257154666 1101 72-73 0.22816411059017128 1101 74-75 0.09672145202399918 1101 76-77 -0.10590795775753037 1101 78-79 0.018118546319211504 1101 80-81 0.0639916321889693 1101 82-83 0.05607780270529261 1101 84-85 -0.04185401557570145 1101 86-87 -0.18676086317134022 1101 88-89 -0.23416100416176988 1101 90-91 -0.15238995894371854 1101 92-93 -0.33079810725363856 1101 94-95 -0.5289827556656554 1101 96-97 -0.6813573148923773 1101 98-99 -0.6847423182577437 1101 100-101 -0.3015221277850131 1102 1 0.36798877204822844 1102 2 0.29148568186653634 1102 3 0.4240451184735008 1102 4 0.3434641113218717 1102 5 0.3383994301941655 1102 6 0.34341415324437463 1102 7 0.34243533783394753 1102 8 0.3996487763454937 1102 9 0.5251835850263831 1102 10-11 0.5010973767947959 1102 12-13 0.5112182233699372 1102 14-15 0.5432880625080472 1102 16-17 0.7270766124651189 1102 18-19 0.6214301864917857 1102 20-21 0.5815799675306579 1102 22-23 0.6309305298249939 1102 24-25 0.6376182983408825 1102 26-27 0.6614268725057997 1102 28-29 0.6587193250031831 1102 30-31 0.6190175522827133 1102 32-33 0.5188165666667146 1102 34-35 0.6340310253633561 1102 36-37 0.5541806529298157 1102 38-39 0.6587796299127362 1102 40-41 0.5029539312761102 1102 42-43 0.4367815751140043 1102 44-45 0.7165695782304766 1102 46-47 0.7376725671969169 1102 48-49 0.6258007173781621 1102 50-51 0.574481969466099 1102 52-53 0.5326452970521771 1102 54-55 0.6282818580184539 1102 56-57 0.6127075304947169 1102 58-59 0.5643398382998583 1102 60-61 0.5978793621083227 1102 62-63 0.5983062272958293 1102 64-65 0.4533733208431414 1102 66-67 0.6652079177983055 1102 68-69 0.7148975938944346 1102 70-71 0.6078436393277542 1102 72-73 0.5203587380610273 1102 74-75 0.43478531416287325 1102 76-77 0.5547610741735411 1102 78-79 0.49708458788028764 1102 80-81 0.49151317298978014 1102 82-83 0.44229169018628767 1102 84-85 0.4551049301921637 1102 86-87 0.42981288239378657 1102 88-89 0.3352764008052844 1102 90-91 0.5052380187552181 1102 92-93 0.6218121309622795 1102 94-95 0.3889087800667319 1102 96-97 0.4210248442561273 1102 98-99 0.3489626994817421 1102 100-101 0.3609208524481353 1103 1 0.3046130153781519 1103 2 0.3823697449760566 1103 3 0.5347501628888978 1103 4 0.3026209555974688 1103 5 0.21308064654333947 1103 6 0.22719107940726246 1103 7 0.31712352099957997 1103 8 0.31750016871167475 1103 9 0.3804967926341405 1103 10-11 0.41770458379807707 1103 12-13 0.36214699841971765 1103 14-15 0.4947957825953466 1103 16-17 0.49526680189260475 1103 18-19 0.6446160883487622 1103 20-21 0.5665835713689802 1103 22-23 0.47018911034002286 1103 24-25 0.5649051946914909 1103 26-27 0.6564800443367602 1103 28-29 0.7590734671899568 1103 30-31 0.6635958539904081 1103 32-33 0.6096953204923139 1103 34-35 0.6681699799420358 1103 36-37 0.6272472771912732 1103 38-39 0.6949065875816913 1103 40-41 0.7695276379928231 1103 42-43 0.6400298986104858 1103 44-45 0.7956236503087908 1103 46-47 0.6659115088002281 1103 48-49 0.7385658934482606 1103 50-51 0.6815176512208865 1103 52-53 0.7973313590941373 1103 54-55 0.8459614626762288 1103 56-57 0.8480581154612565 1103 58-59 0.7651791713398381 1103 60-61 0.798797686933419 1103 62-63 0.850476623712872 1103 64-65 0.7503889076057746 1103 66-67 0.6585592698916329 1103 68-69 0.6170794764939913 1103 70-71 0.7218291737304909 1103 72-73 0.6044128606422916 1103 74-75 0.7089959725632866 1103 76-77 0.772371269656194 1103 78-79 0.6839437993972375 1103 80-81 0.7165707398718624 1103 82-83 0.7130665944184607 1103 84-85 0.595061280108677 1103 86-87 0.7520138991346066 1103 88-89 0.7780658882159379 1103 90-91 0.5294285407251671 1103 92-93 0.3483485276574676 1103 94-95 0.3975568265998852 1103 96-97 0.499479614725427 1103 98-99 0.6999865236631173 1103 100-101 0.880995125445299 1104 1 0.15598178578873245 1104 2 0.2329399112122168 1104 3 0.32375853844467883 1104 4 0.29660733063724365 1104 5 0.2548617998863847 1104 6 0.200893862123678 1104 7 0.23787121729507987 1104 8 0.2547931766334628 1104 9 0.20904837262556697 1104 10-11 0.40847031519601984 1104 12-13 0.2950818160558697 1104 14-15 0.4535643911769043 1104 16-17 0.38323646497300246 1104 18-19 0.36415142219141217 1104 20-21 0.29383502276053974 1104 22-23 0.36252650077665294 1104 24-25 0.3295961735262267 1104 26-27 0.25807469273507877 1104 28-29 0.31925890799836765 1104 30-31 0.30356742348102017 1104 32-33 0.29263678898033163 1104 34-35 0.5038423634969149 1104 36-37 0.41044117687935966 1104 38-39 0.5233999412473835 1104 40-41 0.3529619471525294 1104 42-43 0.3643604966450411 1104 44-45 0.49379343626681305 1104 46-47 0.6300010498462782 1104 48-49 0.6063559935674263 1104 50-51 0.4733481212579207 1104 52-53 0.5164705280438469 1104 54-55 0.5939640738257594 1104 56-57 0.6056744702322518 1104 58-59 0.6704706643732692 1104 60-61 0.7330272873903994 1104 62-63 0.8318879947119413 1104 64-65 0.7618105535435333 1104 66-67 0.7277453210725682 1104 68-69 0.503993661932796 1104 70-71 0.5457153693729495 1104 72-73 0.7078705004883759 1104 74-75 0.7914526064994014 1104 76-77 0.715818950792773 1104 78-79 0.7422479275433425 1104 80-81 0.7315342781451335 1104 82-83 0.8707172575460369 1104 84-85 0.8013304232681371 1104 86-87 0.7590587825966253 1104 88-89 0.6865945892769609 1104 90-91 0.6775128946087072 1104 92-93 0.661061919611992 1104 94-95 0.7517609931954432 1104 96-97 0.6219928235681991 1104 98-99 0.8946534439824028 1104 100-101 1.0421285761682562 1105 1 0.09489074047054658 1105 2 0.1273757287955668 1105 3 0.15739119049430172 1105 4 0.18267580789617455 1105 5 0.16803005990092856 1105 6 0.16338846437771792 1105 7 0.19296602220886427 1105 8 0.268016820142968 1105 9 0.2897814937667178 1105 10-11 0.3478214560864856 1105 12-13 0.28998005245855296 1105 14-15 0.2750671284179731 1105 16-17 0.26714691624838593 1105 18-19 0.21346732640560617 1105 20-21 0.22916061289026857 1105 22-23 0.14688893284607474 1105 24-25 0.3688883085933128 1105 26-27 0.14250168327603774 1105 28-29 0.1791405722485635 1105 30-31 0.11012936672675977 1105 32-33 0.18770377345865086 1105 34-35 0.2728206238069717 1105 36-37 0.2892837047307779 1105 38-39 0.2478856421059774 1105 40-41 0.3706074527485441 1105 42-43 0.37690125975525035 1105 44-45 0.3370700610955879 1105 46-47 0.3361199966256905 1105 48-49 0.35966875588834313 1105 50-51 0.1697859187297226 1105 52-53 0.2700550841513305 1105 54-55 0.32551891540132516 1105 56-57 0.14621261478031755 1105 58-59 0.17024271982539574 1105 60-61 0.3809534090491553 1105 62-63 0.3003993559543616 1105 64-65 0.33162306945080644 1105 66-67 0.3160146726471851 1105 68-69 0.3447449042484507 1105 70-71 0.1861389816160397 1105 72-73 0.15113673495406488 1105 74-75 0.1855294084409671 1105 76-77 0.010972942646347406 1105 78-79 0.015032611576213384 1105 80-81 0.2083219448794793 1105 82-83 0.4368401221596052 1105 84-85 0.6014318722948282 1105 86-87 0.6022968010304623 1105 88-89 0.4169286823460183 1105 90-91 0.42026728453711115 1105 92-93 0.3617080524427756 1105 94-95 0.257500119775802 1105 96-97 0.3081194963928233 1105 98-99 0.5277618593185771 1105 100-101 0.9462826004321379 1106 1 0.1608781252352216 1106 2 0.18074787823758598 1106 3 0.2432718309600972 1106 4 0.2069358758243638 1106 5 0.24178066640262585 1106 6 0.260428736090482 1106 7 0.27884666267465263 1106 8 0.29955490844961474 1106 9 0.20632686009374623 1106 10-11 0.2481125769016188 1106 12-13 0.3183643319345322 1106 14-15 0.4191719319114213 1106 16-17 0.3838378429829845 1106 18-19 0.5036177388267618 1106 20-21 0.38636585306494453 1106 22-23 0.40089184405422884 1106 24-25 0.3727699194618239 1106 26-27 0.45497135819113055 1106 28-29 0.37055250820198893 1106 30-31 0.5150299004482548 1106 32-33 0.5045402606008196 1106 34-35 0.4482209149277878 1106 36-37 0.4215010749394139 1106 38-39 0.5899525998934578 1106 40-41 0.738632683219187 1106 42-43 0.5290118856650068 1106 44-45 0.5493456554672562 1106 46-47 0.5981287302501457 1106 48-49 0.5918376059611248 1106 50-51 0.5407223573517541 1106 52-53 0.4891234975428844 1106 54-55 0.6125155189918132 1106 56-57 0.5387405138584356 1106 58-59 0.5799952671325244 1106 60-61 0.7581974653325148 1106 62-63 0.7358675752653809 1106 64-65 0.49149691709757093 1106 66-67 0.4894741583337421 1106 68-69 0.42480312841147594 1106 70-71 0.3969589719120066 1106 72-73 0.36122892321219524 1106 74-75 0.4062960265874942 1106 76-77 0.3215018121271811 1106 78-79 0.5274052462195904 1106 80-81 0.49313514235643297 1106 82-83 0.6641569586467497 1106 84-85 0.77125234779216 1106 86-87 0.7439755977310938 1106 88-89 0.716297920579887 1106 90-91 0.7742216756094074 1106 92-93 0.7108104299294347 1106 94-95 0.6519688242881791 1106 96-97 0.5742524415650685 1106 98-99 0.7815221698474453 1106 100-101 0.8434199124166142 1107 1 0.14183678195806948 1107 2 0.2157692533244635 1107 3 0.2613301263109662 1107 4 0.2565636383342209 1107 5 0.25573886977131366 1107 6 0.24765543565342085 1107 7 0.12588693438741672 1107 8 0.1904522780587783 1107 9 0.15293012274504036 1107 10-11 0.2126613817888554 1107 12-13 0.34005227065304666 1107 14-15 0.3836458022258995 1107 16-17 0.39426194456429187 1107 18-19 0.2788684652237734 1107 20-21 0.3090457850243524 1107 22-23 0.31200632722111266 1107 24-25 0.359766429384095 1107 26-27 0.4140485342442872 1107 28-29 0.37389751727843645 1107 30-31 0.42594522157100556 1107 32-33 0.38161149902576597 1107 34-35 0.5151186376661201 1107 36-37 0.49366704939796335 1107 38-39 0.6321343246625233 1107 40-41 0.6167693976004429 1107 42-43 0.5433078800374176 1107 44-45 0.322968194100703 1107 46-47 0.6172636246579728 1107 48-49 0.6139774938215368 1107 50-51 0.4534813031347156 1107 52-53 0.5801666617301535 1107 54-55 0.5665691515389 1107 56-57 0.5436912303266155 1107 58-59 0.6323835490513687 1107 60-61 0.5708366797184539 1107 62-63 0.4500572796297533 1107 64-65 0.3978203881044564 1107 66-67 0.35933282704612424 1107 68-69 0.49551642592609113 1107 70-71 0.31958352253874267 1107 72-73 0.416250452851628 1107 74-75 0.380312112230218 1107 76-77 0.3852252361126105 1107 78-79 0.5244686305646376 1107 80-81 0.47803500635017926 1107 82-83 0.47166420856666136 1107 84-85 0.43732129415586485 1107 86-87 0.646566273247533 1107 88-89 0.7833681099208185 1107 90-91 0.8402330628544199 1107 92-93 0.6928986082512374 1107 94-95 0.6290451676308955 1107 96-97 0.7846058470603943 1107 98-99 0.6439702776558143 1107 100-101 0.8920728229690837 1108 1 0.26484559059562685 1108 2 0.20042831358232505 1108 3 0.195061119922201 1108 4 0.26199678747994426 1108 5 0.18242121935785605 1108 6 0.2520626444019527 1108 7 0.30552765295028905 1108 8 0.26918188448298963 1108 9 0.42490644038004177 1108 10-11 0.40828894904815627 1108 12-13 0.45382376241855127 1108 14-15 0.5048767958371343 1108 16-17 0.5485026908709045 1108 18-19 0.5278324099212526 1108 20-21 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-1.8457015005229032 2316 92-93 -1.7254482289418291 2316 94-95 -1.9165977131383194 2316 96-97 -2.267391856610228 2316 98-99 -2.3468039161138385 2316 100-101 -2.016492226486214 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5546.0 3 1468.0 4 1549.0 5 1705.0 6 2026.0 7 2515.0 8 3209.0 9 3952.0 10 5176.0 11 6815.0 12 9324.0 13 13242.0 14 12167.0 15 12700.0 16 15869.0 17 21981.0 18 18645.0 19 13438.0 20 13073.0 21 14521.0 22 15649.0 23 17468.0 24 19648.0 25 22306.0 26 26221.0 27 30760.0 28 36255.0 29 43362.0 30 51887.0 31 63517.0 32 78788.0 33 100998.0 34 136253.0 35 194654.0 36 291201.0 37 422006.0 38 345366.0 39 47413.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.46889511183581 19.060933678352416 14.484373707655408 21.98579750215637 2 15.019200012070735 21.395471211298208 40.034382732814294 23.55094604381677 3 20.260746589531372 25.687363452753768 30.202069882953104 23.849820074761755 4 12.531443204165074 16.550561426840023 38.32783932015713 32.59015604883777 5 13.06555937377078 38.43832272292932 34.183722077532515 14.312395825767387 6 30.191974380161785 36.579163329768214 17.69240450421192 15.536457785858088 7 27.105892823136113 33.874493972927674 21.485056292271054 17.53455691166516 8 25.243941299474127 33.79451987638422 20.373741640002034 20.58779718413962 9 26.033604247128817 17.82336533670479 21.516834414490095 34.6261960016763 10-11 24.655321307290105 26.2882083307407 27.400376921611997 21.6560934403572 12-13 26.95320528170541 24.16938337993699 27.418526884619666 21.45888445373794 14-15 23.50885454339338 25.38711744599234 27.18682675290829 23.917201257705994 16-17 21.25138761551678 28.510873484818134 27.99233541289074 22.245403486774347 18-19 21.1017757155838 28.087019138412 29.71270578890116 21.09849935710304 20-21 22.621554864186333 26.36192573801988 29.055500019210523 21.961019378583263 22-23 21.985848875343223 26.285903828649417 29.235088169291075 22.49315912671629 24-25 22.466857422551005 26.528585561464546 29.301273267235224 21.703283748749225 26-27 22.209582852873574 25.784250601836995 30.309857348541126 21.696309196748302 28-29 21.491223003536543 27.059633690445345 30.029377282244223 21.419766023773885 30-31 21.751391985267198 26.792100732608937 29.38606489495547 22.07044238716839 32-33 21.726805381286844 26.72207802738636 29.45133229821058 22.099784293116215 34-35 22.47347954595508 26.31569619559207 29.262673246644237 21.94815101180861 36-37 22.534982880214528 25.874761383521072 29.94575262667505 21.64450310958935 38-39 22.334200067732606 26.40717331382609 29.800430203820245 21.45819641462106 40-41 21.875537566285548 26.74402178966893 29.819036998284282 21.56140364576124 42-43 21.360278515389332 27.114895172111158 28.83204224026994 22.692784072229575 44-45 21.91264012149939 26.754507227755493 28.71497731828352 22.617875332461605 46-47 22.51223726566938 27.0756248816512 28.15835069115698 22.25378716152244 48-49 22.35496460154993 26.921477111448596 28.417527707380703 22.306030579620767 50-51 22.01718039700935 27.23827023746086 28.983936255410153 21.760613110119635 52-53 22.149762491057608 27.682089794619746 28.190367926989378 21.97777978733327 54-55 21.50911963247836 27.093453568153553 28.63389908020173 22.763527719166355 56-57 22.27743169307089 27.463464285959393 27.750836996982386 22.508267023987326 58-59 22.631774715552687 26.86835140050342 28.069222334798617 22.430651549145274 60-61 22.075605669408148 26.853480369849315 29.02106585705757 22.049848103684962 62-63 22.27433803424953 26.802512288780665 28.826019078852696 22.09713059811711 64-65 21.973693462238952 27.53187819323485 28.523386416898422 21.971041927627784 66-67 21.83620496363375 27.182478094895796 28.049045840249892 22.932271101220557 68-69 21.5457752532177 27.380544753715686 28.470658382598725 22.603021610467895 70-71 22.38746489882313 27.14839092819126 27.794378081379556 22.66976609160605 72-73 21.98893180611767 26.69019143230687 28.34843170596695 22.97244505560851 74-75 21.649892164761923 28.1471161975891 27.64898345547715 22.554008182171824 76-77 22.20654446537705 26.888757438748456 27.940839862247195 22.963858233627295 78-79 21.99701286324229 26.96526358961446 28.346962253260067 22.690761293883185 80-81 22.305141707591268 27.477356369445594 27.73360156203775 22.48390036092539 82-83 22.100951362948017 27.157277143099247 28.193707475183942 22.54806401876879 84-85 22.006794219232866 27.15936117675574 27.820628256983575 23.01321634702781 86-87 22.00030013212441 27.328757817518724 28.013238951090297 22.65770309926657 88-89 22.295665750831862 26.900901655817016 27.692463193216998 23.11096940013412 90-91 22.071562856671395 26.578693739581734 28.134014812198345 23.21572859154853 92-93 22.27669009087545 27.373864530400294 27.829153302571946 22.520292076152316 94-95 22.123205270058037 27.116733007261672 27.74892403218539 23.011137690494902 96-97 21.562982462827648 26.847714992124338 28.073823131036225 23.515479414011793 98-99 22.091931946948208 27.16134354062817 27.310385387411728 23.436339125011898 100-101 22.486638851554314 26.119252126019216 28.14497692305143 23.249132099375036 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2684.0 1 1635.0 2 666.5 3 759.0 4 697.0 5 711.5 6 1161.5 7 2204.5 8 3456.5 9 4014.5 10 3625.5 11 3231.5 12 3298.5 13 3518.5 14 3750.0 15 3964.5 16 4249.5 17 4340.0 18 4351.0 19 4577.5 20 4942.0 21 5448.5 22 6287.5 23 7496.0 24 8469.5 25 9813.5 26 12323.0 27 15285.0 28 17369.5 29 19476.0 30 22397.5 31 25986.5 32 30533.5 33 35161.5 34 39583.0 35 44080.0 36 50955.0 37 56693.5 38 58405.5 39 59413.5 40 59745.0 41 60067.0 42 60332.5 43 62018.0 44 65189.0 45 67713.5 46 72172.5 47 86669.5 48 126222.0 49 125025.0 50 85004.5 51 71640.5 52 67278.5 53 65423.5 54 63735.0 55 59618.5 56 54614.5 57 49768.5 58 46509.5 59 42219.5 60 34972.0 61 28302.5 62 22606.0 63 17137.0 64 11959.0 65 8203.5 66 5980.0 67 4719.5 68 3716.0 69 2792.5 70 2002.5 71 1250.0 72 803.5 73 560.5 74 340.5 75 200.5 76 149.5 77 93.0 78 53.5 79 32.0 80 28.5 81 21.0 82 15.5 83 14.5 84 14.0 85 12.5 86 10.0 87 7.5 88 5.0 89 4.0 90 3.5 91 5.5 92 5.0 93 2.0 94 2.0 95 2.0 96 3.5 97 3.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32275297784494666 2 0.08687147732390624 3 0.09765974647096402 4 0.10298321552606239 5 0.11447813985742525 6 0.05629686301630583 7 0.05879371133418384 8 0.059547476864109265 9 0.06298653209439406 10-11 0.0603012423940347 12-13 0.05841682856922111 14-15 0.05627330784349566 16-17 0.057992835458638065 18-19 0.06682602526245174 20-21 0.06795667355733989 22-23 0.08406841175949603 24-25 0.09374958778447583 26-27 0.08844967390218762 28-29 0.08571727385620792 30-31 0.07982848065366546 32-33 0.07116017705952299 34-35 0.31856015708473645 36-37 0.4946586290135659 38-39 0.46905415616891133 40-41 0.4505869006857382 42-43 0.48003086669845046 44-45 0.4900418151427726 46-47 0.4841059115946098 48-49 0.5016545153381864 50-51 0.4974616945779761 52-53 0.49677859456648116 54-55 0.48829873235482 56-57 0.46707552165285704 58-59 0.4495740282549009 60-61 0.4114617586480461 62-63 0.47958331841505725 64-65 0.5036802601998609 66-67 0.5133378810520306 68-69 0.5254216847036477 70-71 0.5229955019042002 72-73 0.5115712430912679 74-75 0.5130081086326882 76-77 0.4871916392327421 78-79 0.46877149409518926 80-81 0.4735060838300334 82-83 0.4820801667329352 84-85 0.48485967712453526 86-87 0.4839645805577488 88-89 0.48973559789624044 90-91 0.504104253310444 92-93 0.5101814878954678 94-95 0.5169182673191763 96-97 0.5083441844162745 98-99 0.5112885810175458 100-101 0.4897120427234302 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2122676.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.830978163039276 #Duplication Level Percentage of deduplicated Percentage of total 1 71.34705560363129 31.27211236159912 2 14.422500221821569 12.643045845581804 3 5.423615969934662 7.1316717942875165 4 2.692815835261777 4.721150082897813 5 1.5391012099377681 3.373015576174482 6 0.9577247436315226 2.518680739458939 7 0.6825846363862975 2.094284660431174 8 0.47199535180337787 1.6550414366359918 9 0.34563333842916827 1.3634502578196515 >10 1.8891428059242286 15.19669109106983 >50 0.14733986806820754 4.454746796130515 >100 0.07304732289465472 5.704647988227047 >500 0.0026967725636250204 0.7877466040396917 >1k 0.0036676106865300264 3.356847331709382 >5k 8.629672203600062E-4 2.558793497630638 >10k+ 2.1574180509000156E-4 1.1680739363063788 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14617 0.688611921932504 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10088 0.475249166617986 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9197 0.4332738486702634 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8065 0.379944937428039 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7782 0.3666127096174828 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7595 0.35780307498647934 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5950 0.2803065564410207 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5191 0.24454980411518293 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5185 0.24426714204146088 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5154 0.24280672132723033 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4185 0.19715679642112127 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3475 0.16370845103068013 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3439 0.1620124785883479 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3298 0.15536991985588003 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3291 0.15504014743653766 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3112 0.14660739557049685 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3062 0.14425187828947988 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3022 0.1423674644646663 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2992 0.1409541540960561 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2773 0.13063698840520171 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2478 0.11673943644720156 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2369 0.11160440877458454 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2303 0.10849512596364211 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.711034562033961E-5 2 0.0 0.0 0.0 0.0 4.711034562033961E-5 3 0.0 0.0 0.0 0.0 4.711034562033961E-5 4 0.0 0.0 0.0 0.0 4.711034562033961E-5 5 9.422069124067921E-5 0.0 0.0 0.0 4.711034562033961E-5 6 9.422069124067921E-5 0.0 0.0 0.0 4.711034562033961E-5 7 9.422069124067921E-5 0.0 0.0 0.0 4.711034562033961E-5 8 9.422069124067921E-5 0.0 0.0 0.0 9.422069124067921E-5 9 9.422069124067921E-5 0.0 0.0 0.0 9.422069124067921E-5 10-11 9.422069124067921E-5 0.0 0.0 0.0 9.422069124067921E-5 12-13 9.422069124067921E-5 0.0 0.0 0.0 9.422069124067921E-5 14-15 9.422069124067921E-5 0.0 0.0 4.711034562033961E-5 2.5910690091186783E-4 16-17 9.422069124067921E-5 0.0 0.0 2.1199655529152825E-4 3.2977241934237727E-4 18-19 9.422069124067921E-5 0.0 0.0 2.8266207372203763E-4 3.2977241934237727E-4 20-21 9.422069124067921E-5 0.0 0.0 3.062172465322074E-4 3.2977241934237727E-4 22-23 1.4133103686101881E-4 0.0 0.0 7.066551843050941E-4 3.768827649627169E-4 24-25 2.3555172810169804E-4 0.0 0.0 0.0013662000229898487 4.239931105830565E-4 26-27 2.3555172810169804E-4 0.0 0.0 0.0021199655529152827 4.239931105830565E-4 28-29 2.5910690091186783E-4 0.0 0.0 0.006100789757833979 4.239931105830565E-4 30-31 2.8266207372203763E-4 0.0 0.0 0.016771283040840898 4.239931105830565E-4 32-33 2.8266207372203763E-4 0.0 0.0 0.03896025582802086 5.653241474440753E-4 34-35 3.2977241934237727E-4 0.0 0.0 0.07177261155258739 5.653241474440753E-4 36-37 3.2977241934237727E-4 0.0 0.0 0.12168602273733721 5.653241474440753E-4 38-39 3.7688276496271686E-4 0.0 0.0 0.19110311700890764 5.653241474440753E-4 40-41 3.7688276496271686E-4 0.0 0.0 0.26544324239780354 5.653241474440753E-4 42-43 4.004379377728867E-4 0.0 0.0 0.345271723051469 5.653241474440753E-4 44-45 4.239931105830565E-4 0.0 0.0 0.43984574188430076 5.653241474440753E-4 46-47 6.124344930644148E-4 0.0 0.0 0.5584695921563159 5.653241474440753E-4 48-49 6.595448386847545E-4 0.0 0.0 0.6850786460109786 5.653241474440753E-4 50-51 6.595448386847545E-4 0.0 0.0 0.8213217655450007 5.653241474440753E-4 52-53 6.595448386847545E-4 0.0 0.0 0.9549738160699042 6.124344930644148E-4 54-55 6.595448386847545E-4 0.0 0.0 1.107375784151703 6.595448386847545E-4 56-57 7.066551843050941E-4 0.0 0.0 1.2915536803544208 6.595448386847545E-4 58-59 7.066551843050941E-4 0.0 0.0 1.5028906908072641 6.595448386847545E-4 60-61 7.066551843050941E-4 0.0 0.0 1.7377828740702772 7.066551843050941E-4 62-63 7.066551843050941E-4 0.0 0.0 1.9753132366880295 7.066551843050941E-4 64-65 7.066551843050941E-4 0.0 0.0 2.2127729337873516 7.066551843050941E-4 66-67 7.066551843050941E-4 0.0 0.0 2.4895226591340363 7.066551843050941E-4 68-69 7.066551843050941E-4 0.0 0.0 2.8078708196634814 7.066551843050941E-4 70-71 7.302103571152639E-4 0.0 0.0 3.1535429806527233 7.302103571152639E-4 72-73 7.773207027356036E-4 0.0 0.0 3.511416721157633 8.950965667864526E-4 74-75 8.008758755457734E-4 0.0 0.0 3.897438893170696 8.950965667864526E-4 76-77 8.008758755457734E-4 0.0 0.0 4.287488999734298 8.950965667864526E-4 78-79 8.008758755457734E-4 0.0 0.0 4.756142717965436 8.950965667864526E-4 80-81 8.244310483559432E-4 0.0 0.0 5.268773943833162 9.65762085216962E-4 82-83 8.950965667864526E-4 0.0 0.0 5.773584852327911 0.001083537949267811 84-85 8.950965667864526E-4 0.0 0.0 6.33382108244499 0.001083537949267811 86-87 9.422069124067922E-4 4.711034562033961E-5 0.0 6.928235868309624 0.001083537949267811 88-89 9.893172580271318E-4 4.711034562033961E-5 0.0 7.532991375037923 0.001083537949267811 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 675 0.0 24.558994 3 GTACATG 10930 0.0 22.622223 1 TACATGG 10770 0.0 22.560461 2 GAGTACT 7990 0.0 21.835236 12-13 ACATGGG 11145 0.0 20.866411 3 GTGTACG 205 6.891896E-7 20.835619 1 GTACTTT 8380 0.0 20.481262 14-15 CATGGGG 4190 0.0 20.460247 4 CTTTGCG 820 0.0 19.63864 2 GTAAGCG 280 0.0 19.535444 94-95 AGTACTT 8505 0.0 19.06573 12-13 CATGGGA 6555 0.0 18.280756 4 GTATAGG 670 0.0 17.708548 1 CTATACG 190 1.9080561E-4 17.449812 4 ACTTTTT 10080 0.0 17.310745 16-17 ATGGGAT 2690 0.0 16.726984 5 AGTACAT 8305 0.0 16.310705 2 GTCTAGA 675 0.0 16.171183 1 TACTTTT 9890 0.0 16.061605 14-15 TACCTGG 1260 0.0 15.412024 2 >>END_MODULE