FastQCFastQC Report
Thu 26 May 2016
SRR938913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122676
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT150920.7109893361021653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98390.4635186905585214No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA88780.4182456484173751No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86520.40759871030717826No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT75950.35780307498647934No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA75200.35426979906495387No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT57200.26947117694834255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53840.25364210081990846No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC49570.23352598324002344No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT47310.2228790451298267No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT42500.20021896888644336No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC36790.17331896153722942No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35830.1687963683576768No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35510.16728883729782595No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG34490.16248358204455132No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA33540.15800809921061906No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG33440.15753699575441565No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA32210.15174242324311388No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT30810.14514697485626635No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC27690.13044854702272038No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC27300.12861124354352713No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA24810.11688076748406256No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG23040.10854223630926245No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT5000.029.422313
GTAAGCG3750.025.3101194-95
GTACATG101600.022.5978971
TATCCGG1050.00220887222.597785
TACATGG100500.022.3347032
GAGTACT88550.022.29671912-13
AGTACTT94450.020.7782912-13
GTACTTT94100.020.7294814-15
ACATGGG101150.020.689873
CTTTGCG7150.020.575042
GTCGTAA1908.058427E-620.0563131
GTACCGT1456.1874604E-419.6366926
CGTATAG2702.228444E-819.406341
CATGGGA61100.018.9510674
CATGGGG36700.018.1028564
ACTTTTT109150.017.9132316-17
TACTTTT108950.017.62087614-15
CTATACA9450.017.5760524
CCCTATA5700.017.4835472
AGTACAT80200.016.567952
Produced by FastQC (version 0.11.5)