##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938913_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2122676 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.141054499132224 33.0 31.0 34.0 30.0 34.0 2 32.380501310609816 34.0 31.0 34.0 30.0 34.0 3 32.46392431063431 34.0 31.0 34.0 30.0 34.0 4 36.014678170384926 37.0 35.0 37.0 35.0 37.0 5 35.94711392600661 37.0 35.0 37.0 35.0 37.0 6 35.996938298638135 37.0 35.0 37.0 35.0 37.0 7 35.93697813514639 37.0 35.0 37.0 35.0 37.0 8 35.91299520039799 37.0 35.0 37.0 35.0 37.0 9 37.56991505062478 39.0 37.0 39.0 35.0 39.0 10-11 37.47935883761818 39.0 37.0 39.0 34.0 39.0 12-13 37.389748129248176 39.0 37.0 39.0 34.0 39.0 14-15 38.64778727417656 40.0 38.0 41.0 34.0 41.0 16-17 38.754586427697866 40.0 38.0 41.0 34.5 41.0 18-19 38.8307155684617 40.0 38.0 41.0 34.5 41.0 20-21 38.79926305286346 40.0 38.0 41.0 34.0 41.0 22-23 38.72638287708534 40.0 38.0 41.0 34.0 41.0 24-25 38.63570818155951 40.0 38.0 41.0 34.0 41.0 26-27 38.44644613685744 40.0 38.0 41.0 34.0 41.0 28-29 38.281328379837525 40.0 38.0 41.0 34.0 41.0 30-31 38.09007286086053 40.0 37.5 41.0 33.0 41.0 32-33 37.9727405407137 40.0 37.0 41.0 33.0 41.0 34-35 37.85701562555944 40.0 37.0 41.0 33.0 41.0 36-37 37.70579282942852 40.0 37.0 41.0 33.0 41.0 38-39 37.53338286201003 40.0 37.0 41.0 32.5 41.0 40-41 37.36213369350763 40.0 36.5 41.0 32.0 41.0 42-43 37.16687191073909 40.0 36.0 41.0 31.0 41.0 44-45 36.89766408062276 40.0 36.0 41.0 31.0 41.0 46-47 36.547349195072634 39.0 36.0 41.0 30.0 41.0 48-49 36.163866977343694 39.0 35.0 41.0 29.5 41.0 50-51 35.88414011370553 39.0 35.0 40.0 28.5 41.0 52-53 35.60350920253491 39.0 35.0 40.0 28.0 41.0 54-55 35.31031301998044 38.0 35.0 40.0 27.0 41.0 56-57 35.16580462585905 38.0 34.5 40.0 27.0 41.0 58-59 34.881160619896775 38.0 34.0 40.0 26.5 41.0 60-61 34.48076178371075 37.5 34.0 40.0 26.0 41.0 62-63 34.04452587205961 37.0 33.5 40.0 25.5 41.0 64-65 33.59280219873405 36.5 33.0 39.0 24.0 41.0 66-67 33.1077646800548 36.0 32.0 39.0 22.5 40.0 68-69 32.59823873261864 35.0 32.0 38.5 22.0 40.0 70-71 32.03143814694282 35.0 31.0 37.5 20.5 40.0 72-73 31.48414336431938 35.0 31.0 37.0 18.5 39.0 74-75 30.954856982412764 34.0 30.0 36.5 17.5 39.0 76-77 29.055604576487415 32.5 28.0 34.5 11.5 36.5 78-79 30.010777433767565 34.0 29.5 35.0 11.5 37.0 80-81 29.993475452683313 34.0 30.0 35.0 7.5 37.0 82-83 29.564652589467258 34.0 29.0 35.0 6.5 36.5 84-85 29.09551881681425 34.0 29.0 35.0 2.0 36.0 86-87 28.39471567964211 33.5 28.5 35.0 2.0 35.5 88-89 27.645768360315 33.0 26.5 35.0 2.0 35.0 90-91 27.016172510548007 32.5 25.5 34.5 2.0 35.0 92-93 26.593471636745313 32.0 25.0 34.0 2.0 35.0 94-95 25.987976026487324 32.0 24.5 34.0 2.0 35.0 96-97 25.04069509430549 31.5 20.0 34.0 2.0 35.0 98-99 23.72317631141069 31.0 2.0 34.0 2.0 35.0 100-101 18.049209111517726 23.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15413237802182067 1101 2 0.10389718771070733 1101 3 0.12617233082637824 1101 4 0.0806376971630911 1101 5 0.1172741135349753 1101 6 0.096656063075514 1101 7 0.15442063205421164 1101 8 0.07768887737941554 1101 9 0.13867638398362203 1101 10-11 0.21203422436334307 1101 12-13 0.13980074696281264 1101 14-15 0.11532955809828849 1101 16-17 0.24836138105586514 1101 18-19 0.14810772163615127 1101 20-21 0.269446725570738 1101 22-23 0.22492785383892056 1101 24-25 0.16242595328940723 1101 26-27 0.15654386071796011 1101 28-29 0.16526965549043382 1101 30-31 0.058281221918143444 1101 32-33 0.12248267993608408 1101 34-35 0.38625046095787496 1101 36-37 0.3251322401722021 1101 38-39 0.24342186236696506 1101 40-41 0.2624183332162957 1101 42-43 0.2390485560568436 1101 44-45 0.3582162088489014 1101 46-47 0.19391042198032693 1101 48-49 0.09476805082520201 1101 50-51 -0.0721285433814316 1101 52-53 -0.045446619384044595 1101 54-55 -0.033770035644757 1101 56-57 -0.07247067585292655 1101 58-59 0.11898094812485027 1101 60-61 -0.01002967260128429 1101 62-63 0.0638184990016768 1101 64-65 0.1408315142753338 1101 66-67 0.057259145295589065 1101 68-69 0.13433190622838254 1101 70-71 0.15334230709535035 1101 72-73 0.182536522197438 1101 74-75 0.2858693419907219 1101 76-77 -0.048771058068577844 1101 78-79 0.35143217932422033 1101 80-81 0.20866852437957917 1101 82-83 0.1743470379716321 1101 84-85 0.12007540629864266 1101 86-87 0.2738840185334688 1101 88-89 0.307125818986556 1101 90-91 0.5303199102944305 1101 92-93 0.4694329380117317 1101 94-95 0.3821649405494796 1101 96-97 0.5724857302964317 1101 98-99 0.9693655136927468 1101 100-101 1.0819440744144018 1102 1 0.25448716768223534 1102 2 0.2655799043858238 1102 3 0.21284237644219672 1102 4 0.1678145851509285 1102 5 0.17353361682945234 1102 6 0.2335035035215327 1102 7 0.2542685793426074 1102 8 0.2515358109830643 1102 9 0.1878400940697844 1102 10-11 0.3202450707901008 1102 12-13 0.3493800677940371 1102 14-15 0.39637365338465713 1102 16-17 0.4006675138485676 1102 18-19 0.37035810176792694 1102 20-21 0.3064462187283752 1102 22-23 0.2690231402048653 1102 24-25 0.34564947077547004 1102 26-27 0.3118910477427903 1102 28-29 0.284631034101686 1102 30-31 0.3296953121360815 1102 32-33 0.40124598454834626 1102 34-35 0.32644306105924414 1102 36-37 0.3476867257271934 1102 38-39 0.4978567331221555 1102 40-41 0.48365502860403353 1102 42-43 0.5598797775469535 1102 44-45 0.4649065281595881 1102 46-47 0.6047061645043996 1102 48-49 0.5988227898013818 1102 50-51 0.5690270572267835 1102 52-53 0.5777160770173708 1102 54-55 0.7622259096162693 1102 56-57 0.7377168558247149 1102 58-59 0.6572475472125348 1102 60-61 0.7748157404752476 1102 62-63 0.8914920925140919 1102 64-65 0.8423013571541986 1102 66-67 0.8116940160507866 1102 68-69 0.7014885205213375 1102 70-71 0.7496423577795852 1102 72-73 0.6009349003018514 1102 74-75 0.6485150591726772 1102 76-77 0.511036341830053 1102 78-79 0.7979603090758687 1102 80-81 0.7963522547394355 1102 82-83 0.7308599624521186 1102 84-85 1.0420216810072098 1102 86-87 1.1998850322182122 1102 88-89 0.9921232847746957 1102 90-91 1.1223118008164796 1102 92-93 1.068520621742092 1102 94-95 1.3489667651820199 1102 96-97 1.3274274434236482 1102 98-99 1.8107238512497652 1102 100-101 1.6379501565228622 1103 1 0.23642783267911227 1103 2 0.27203029586134164 1103 3 0.22110336322829482 1103 4 0.14946492296382985 1103 5 0.1260797551541657 1103 6 0.18854396932961492 1103 7 0.20178643731330936 1103 8 0.2029053601937818 1103 9 0.253043021375845 1103 10-11 0.23365336948575788 1103 12-13 0.22152616581998785 1103 14-15 0.24189434003823607 1103 16-17 0.15641636762596534 1103 18-19 0.30733172187856184 1103 20-21 0.3056709337816912 1103 22-23 0.2893500574196537 1103 24-25 0.21234215724982164 1103 26-27 0.19726205402820085 1103 28-29 0.13029882676819682 1103 30-31 0.26419856128834596 1103 32-33 0.30878221523128957 1103 34-35 0.3621972034014007 1103 36-37 0.2702496543385635 1103 38-39 0.3742568625132918 1103 40-41 0.525874065831438 1103 42-43 0.5752518379194669 1103 44-45 0.5174385256598271 1103 46-47 0.5898277380414783 1103 48-49 0.767176556971414 1103 50-51 0.63266827809273 1103 52-53 0.5844876154225247 1103 54-55 0.9048036213459696 1103 56-57 0.901161218802585 1103 58-59 0.8294734657898815 1103 60-61 0.8060650201608084 1103 62-63 0.815864709972935 1103 64-65 0.8863572771223147 1103 66-67 0.6740508304907991 1103 68-69 0.7496328480953522 1103 70-71 0.762447832609574 1103 72-73 0.7402989413247383 1103 74-75 0.7249836394779265 1103 76-77 0.5156457067335118 1103 78-79 0.7284797823638272 1103 80-81 0.8460399005019426 1103 82-83 0.8667648107606993 1103 84-85 0.963168455254646 1103 86-87 1.0415471644110923 1103 88-89 0.8948538365439695 1103 90-91 0.9057519370863716 1103 92-93 1.0299165593888269 1103 94-95 1.3656599307868724 1103 96-97 0.9534351390364435 1103 98-99 1.2525654372390598 1103 100-101 1.446057873346895 1104 1 0.01420455078238092 1104 2 0.0898285777831731 1104 3 0.046845773978880345 1104 4 0.059678171862302065 1104 5 0.027955094598588914 1104 6 0.10196819931155687 1104 7 0.08041592011294796 1104 8 0.06482464043607905 1104 9 0.1634683446072458 1104 10-11 0.09708929492794738 1104 12-13 0.1207987634068104 1104 14-15 0.14064578691223772 1104 16-17 0.1387252271629862 1104 18-19 0.1415266124180974 1104 20-21 0.287876893346521 1104 22-23 0.19800073259406048 1104 24-25 0.23122756482908358 1104 26-27 0.08527400648740979 1104 28-29 0.2157236229509607 1104 30-31 -0.009368418562331726 1104 32-33 0.16805131923857175 1104 34-35 0.15985833722858445 1104 36-37 0.20317217417316158 1104 38-39 0.12585745903196255 1104 40-41 0.2259890377271958 1104 42-43 0.1617573347857899 1104 44-45 0.25133756623051795 1104 46-47 0.16224472286147318 1104 48-49 0.009876807846559643 1104 50-51 -0.0926683554016634 1104 52-53 -0.10907388312231348 1104 54-55 0.06625192088629461 1104 56-57 -0.07785961015959231 1104 58-59 0.05797490086802526 1104 60-61 0.013603346635818525 1104 62-63 -0.08518780615725774 1104 64-65 -0.08619503370989179 1104 66-67 -0.2384451664493099 1104 68-69 -0.2012853710223581 1104 70-71 -0.12114594640947729 1104 72-73 -0.16691050680011443 1104 74-75 -0.14861742285134838 1104 76-77 -0.12427165204003998 1104 78-79 0.022291829792180806 1104 80-81 0.036466463552518036 1104 82-83 -0.0704767471750003 1104 84-85 0.1750918263982797 1104 86-87 0.44625150016416626 1104 88-89 0.44599876186870446 1104 90-91 0.6254546716154898 1104 92-93 0.4842264619801995 1104 94-95 0.6599589962543426 1104 96-97 0.5388832759446025 1104 98-99 0.48647928536673035 1104 100-101 0.6403320169430557 1105 1 -0.08351300101929837 1105 2 0.006759531437381838 1105 3 -0.031416299575354856 1105 4 0.020801770473994452 1105 5 -0.026723714494217177 1105 6 -0.046010350784769116 1105 7 0.023260105656234487 1105 8 -0.01317362275276679 1105 9 0.0401876398927854 1105 10-11 0.10325321100813056 1105 12-13 0.07931964755997711 1105 14-15 0.03634846619603849 1105 16-17 -0.0796831836929428 1105 18-19 0.007128814984419307 1105 20-21 0.03114043489701146 1105 22-23 0.016942221504493205 1105 24-25 -0.07315049568096299 1105 26-27 -0.09843929003162089 1105 28-29 0.0099608320803668 1105 30-31 0.04935774675501392 1105 32-33 0.09046664492878875 1105 34-35 -0.01712302875146321 1105 36-37 0.042492120143272416 1105 38-39 0.055773864271372986 1105 40-41 0.05804871749429452 1105 42-43 0.09434985429631837 1105 44-45 -0.07981843792180143 1105 46-47 -0.13588386789071905 1105 48-49 -0.17632314563511642 1105 50-51 -0.14106623767408166 1105 52-53 -0.23372761712330004 1105 54-55 -0.10331761304396281 1105 56-57 0.014168383766339332 1105 58-59 0.029516506646210416 1105 60-61 0.029739747318721754 1105 62-63 -0.014492705165785935 1105 64-65 -0.09301930979232509 1105 66-67 -0.16997875067654888 1105 68-69 -0.24960389570519226 1105 70-71 -0.07681651089216501 1105 72-73 -0.048286281430282685 1105 74-75 -0.07386221599256615 1105 76-77 -0.2120897615849735 1105 78-79 -0.15007285479357435 1105 80-81 -0.1443624348116188 1105 82-83 0.1189910469890485 1105 84-85 0.03995311981736549 1105 86-87 0.0018111070080806257 1105 88-89 0.12965831923923687 1105 90-91 0.019057115352623555 1105 92-93 0.3910654180251498 1105 94-95 0.5284054415586148 1105 96-97 0.6919185488441535 1105 98-99 0.9460892511547918 1105 100-101 0.6675147319544621 1106 1 0.0319649223188847 1106 2 0.13339708602254063 1106 3 0.04912712594623514 1106 4 0.01692015960548332 1106 5 0.067890550425723 1106 6 0.09324243912304553 1106 7 0.16833531186681228 1106 8 0.04796174842627465 1106 9 0.04503965347842609 1106 10-11 0.1403711149382687 1106 12-13 0.11910615896221799 1106 14-15 0.0739515714847343 1106 16-17 -0.0076307819462115845 1106 18-19 0.094465059525902 1106 20-21 0.15389637861365202 1106 22-23 0.02761665139289704 1106 24-25 0.17551520058299275 1106 26-27 0.07113858478642499 1106 28-29 0.17395889127493547 1106 30-31 0.16410786805536048 1106 32-33 0.2069149709841014 1106 34-35 0.15730685965223046 1106 36-37 0.15141982514084873 1106 38-39 0.1967248589341537 1106 40-41 0.27226511730021485 1106 42-43 0.25470890330165474 1106 44-45 0.22998262952118154 1106 46-47 0.30686237179875064 1106 48-49 0.3134876258350445 1106 50-51 0.32957908013280957 1106 52-53 0.31600552212948685 1106 54-55 0.36805550680076493 1106 56-57 0.370063580272884 1106 58-59 0.3007440660833751 1106 60-61 0.40843940767776843 1106 62-63 0.44596338411125913 1106 64-65 0.39436545488501196 1106 66-67 0.5012973288964702 1106 68-69 0.39377310575040525 1106 70-71 0.4780112426255485 1106 72-73 0.4733051790258074 1106 74-75 0.42953419351738376 1106 76-77 0.3827671040142526 1106 78-79 0.4244055537459701 1106 80-81 0.5070203890602905 1106 82-83 0.3819701833306297 1106 84-85 0.6171001358290127 1106 86-87 0.7538732127819756 1106 88-89 0.9287849567938231 1106 90-91 1.1621915161289458 1106 92-93 1.0473002554826927 1106 94-95 1.233403015551822 1106 96-97 1.256207728853859 1106 98-99 1.6510868251480026 1106 100-101 1.655384697990364 1107 1 0.07678656866891487 1107 2 0.04032296074316832 1107 3 0.11868490893196793 1107 4 0.16341420511699312 1107 5 0.08222248997600445 1107 6 0.08589838265235272 1107 7 0.06634771915869919 1107 8 0.06885631735577391 1107 9 0.039685812425894085 1107 10-11 0.09911319294889864 1107 12-13 0.03323921747300318 1107 14-15 0.06803103772577401 1107 16-17 0.08789452345900628 1107 18-19 0.017496130209813998 1107 20-21 0.20979159853725804 1107 22-23 0.13112202045503807 1107 24-25 0.15273075831164817 1107 26-27 0.08896528547338534 1107 28-29 0.07222408566458682 1107 30-31 0.1830687737520762 1107 32-33 0.03878544095501013 1107 34-35 0.13774811656728758 1107 36-37 0.22557917631984736 1107 38-39 0.08236474866793486 1107 40-41 0.1367033234414805 1107 42-43 0.1584491219982027 1107 44-45 0.2199678580435318 1107 46-47 0.07413760086436838 1107 48-49 0.020083530847983866 1107 50-51 -0.07416312901333555 1107 52-53 -0.10549875663461705 1107 54-55 -0.2157824640459367 1107 56-57 -0.05352352835788565 1107 58-59 -0.14100191414513574 1107 60-61 -0.035658113111885825 1107 62-63 0.007375779038937935 1107 64-65 0.07557458041728893 1107 66-67 0.10820414213516472 1107 68-69 0.1255767668662955 1107 70-71 0.2306700149349723 1107 72-73 0.10685287207131466 1107 74-75 0.1884296298145678 1107 76-77 0.025076756149232438 1107 78-79 0.30148545648089353 1107 80-81 0.3248683758493236 1107 82-83 0.16506866702955136 1107 84-85 0.3450580141689841 1107 86-87 0.4092495810404415 1107 88-89 0.817964100066856 1107 90-91 0.8099764267413683 1107 92-93 0.8002944770904534 1107 94-95 0.8064600089144918 1107 96-97 0.8704683166406504 1107 98-99 0.7676128958225412 1107 100-101 0.5590646177729042 1108 1 0.12579472995252416 1108 2 0.08764434114838338 1108 3 0.11122781539702942 1108 4 0.13089526616093394 1108 5 0.03025403306742902 1108 6 0.1098620383525315 1108 7 0.1442989595350852 1108 8 0.2333304578778197 1108 9 0.21707331541220043 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5162.0 13 6902.0 14 8554.0 15 11597.0 16 14997.0 17 20950.0 18 21042.0 19 14565.0 20 13765.0 21 14842.0 22 16208.0 23 17623.0 24 20086.0 25 23758.0 26 28196.0 27 33647.0 28 40821.0 29 49531.0 30 59190.0 31 71731.0 32 88976.0 33 113405.0 34 152424.0 35 217572.0 36 330628.0 37 454585.0 38 253409.0 39 10357.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29693300858379 19.812622667833242 15.020690959824067 21.8697533637589 2 15.424680921629113 21.938487079516612 39.13239703091757 23.5044349679367 3 20.239970678520887 25.81887202757274 30.4571682159689 23.483989077937473 4 12.931648541746362 17.150332881702155 37.80605236032253 32.11196621622895 5 12.95661702492514 38.64579427100509 34.27499062504123 14.122598079028547 6 30.313858544591827 36.83614456469099 17.34127111250139 15.508725778215798 7 27.012883737320248 34.08235642179965 21.226885308921382 17.677874531958714 8 25.271261370081916 34.198813196173134 20.08756871043909 20.442356723305867 9 25.833476234715047 17.780433754374194 21.354931228317465 35.031158782593295 10-11 24.71239133998783 26.47314050754802 27.25448914483416 21.55997900762999 12-13 26.876004628120352 24.229439631860917 27.417066947569957 21.477488792448778 14-15 23.351446642450156 25.554487972971607 27.154911781534132 23.93915360304411 16-17 21.131527112394217 28.671923988529308 27.989797548038354 22.206751351038122 18-19 21.028010085439806 28.180272525468 29.690049861871685 21.10166752722051 20-21 22.48915990947276 26.428126572307786 29.183068918666816 21.89964459955264 22-23 21.862639423067865 26.348792750283135 29.341736562716118 22.446831263932886 24-25 22.43292072502856 26.619242229733707 29.368297077979438 21.57953996725829 26-27 22.131738930420592 25.89343024875917 30.344134089266117 21.630696731554117 28-29 21.48182294424585 27.15541608799459 30.035483512321235 21.327277455438324 30-31 21.750399024627402 26.784681223135326 29.438430547101866 22.026489205135405 32-33 21.663221330056967 26.75132709843612 29.55714861806512 22.028302953441788 34-35 22.449870959610536 26.312083386830228 29.363820867674413 21.87422478588483 36-37 22.47175604137162 25.96686566805494 29.989640367367603 21.571737923205838 38-39 22.2913484899134 26.438359165575353 29.90002801015527 21.370264334355976 40-41 21.824901015194946 26.787568526129917 29.851668843295887 21.53586161537925 42-43 21.342898205161365 27.089806947605922 28.978565255091908 22.588729592140805 44-45 21.957315819265332 26.750388019804618 28.782412815383136 22.509883345546918 46-47 22.483606584407895 27.148033284793406 28.17088160041382 22.19747853038488 48-49 22.34182610688688 26.97628884459852 28.441396517539623 22.24048853097498 50-51 21.992788307523 27.26415956552709 28.92306554405219 21.81998658289772 52-53 22.152862707261967 27.732046718387544 28.104242003961037 22.010848570389452 54-55 21.582968856292716 27.14019944635922 28.485529586239256 22.79130211110881 56-57 22.272146102372663 27.517105766494744 27.674548541557918 22.536199589574668 58-59 22.58273702935799 26.960053404715502 28.03676897702363 22.42044058890288 60-61 22.044294275372064 27.02765347659488 28.91124726726496 22.016804980768093 62-63 22.20976135675976 26.937069802753218 28.752328167845263 22.10084067264176 64-65 21.879153843707712 27.688993631803527 28.375208906759568 22.05664361772919 66-67 21.802651254253004 27.294854750124607 27.929236442920956 22.97325755270143 68-69 21.46154614434412 27.536626869974967 28.291919145283522 22.709907840397396 70-71 22.32458517564272 27.269382437400346 27.672034656262817 22.733997730694117 72-73 21.910998192847142 26.809955923560636 28.157759356585743 23.121286527006475 74-75 21.560520776604626 28.27431035165046 27.401426312824 22.76374255892091 76-77 21.993326939417173 27.001470223494717 27.834188213126765 23.17101462396134 78-79 21.77393709115493 27.129009537853428 28.179728115820495 22.917325255171146 80-81 22.083272482298238 27.559535867563024 27.594962947608437 22.762228702530305 82-83 21.838071377826857 27.262121020824658 28.033105382074325 22.866702219274163 84-85 21.677793745578985 27.323878437320463 27.698735722316354 23.299592094784195 86-87 21.633294737941533 27.425975005181023 27.821548299999126 23.119181956878315 88-89 21.869884156000477 27.009811065079685 27.551368988025732 23.568935790894106 90-91 21.61112256653658 26.683892368605743 27.967969369243256 23.73701569561442 92-93 21.75817223165476 27.501111804156643 27.619829875119894 23.120886089068705 94-95 21.541353055808187 27.30192313445264 27.5402332315504 23.616490578188774 96-97 20.89647689991313 27.035190485971484 27.89210411763265 24.17622849648274 98-99 21.33669952456239 27.314649056191335 27.144957591266873 24.2036938279794 100-101 21.548622558319323 26.20632440227318 28.275843549647682 23.969209489759816 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1380.0 1 940.0 2 570.0 3 662.5 4 608.5 5 598.0 6 1000.5 7 1953.5 8 3065.5 9 3591.0 10 3258.5 11 2826.0 12 2845.0 13 3037.0 14 3260.0 15 3434.0 16 3578.0 17 3718.5 18 3836.5 19 4092.0 20 4568.5 21 5260.5 22 6253.5 23 7400.0 24 8466.0 25 9954.0 26 12319.5 27 15172.5 28 17392.5 29 19679.5 30 22622.5 31 26188.0 32 30802.5 33 35314.0 34 39683.5 35 44379.5 36 51062.0 37 56968.0 38 58957.0 39 59803.0 40 59976.5 41 60260.5 42 60565.0 43 62032.5 44 65463.5 45 68267.5 46 72035.0 47 87209.0 48 126489.0 49 125820.5 50 88093.0 51 73756.0 52 68045.0 53 65869.0 54 64026.0 55 59916.0 56 54779.5 57 49689.5 58 46110.5 59 42110.0 60 35056.5 61 28104.0 62 22366.0 63 16732.5 64 11433.5 65 7755.0 66 5674.0 67 4491.0 68 3474.5 69 2597.5 70 1859.0 71 1101.5 72 653.5 73 468.0 74 297.0 75 142.0 76 79.5 77 31.5 78 11.5 79 5.5 80 4.0 81 3.0 82 3.0 83 3.0 84 1.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3875297030729136 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.186517395966225E-4 16-17 0.002308406935396641 18-19 5.653241474440754E-4 20-21 0.0 22-23 0.0 24-25 4.711034562033961E-5 26-27 2.3555172810169803E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013120231255264582 36-37 0.023390286600498616 38-39 0.010906045011108619 40-41 0.00603012423940347 42-43 0.005535465610389904 44-45 0.0018608586520034145 46-47 0.00117775864050849 48-49 0.003179948329372923 50-51 5.653241474440754E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.008758755457734E-4 60-61 0.0014839758870406976 62-63 0.001436865541420358 64-65 8.715413939762828E-4 66-67 0.0027324000459796973 68-69 0.002543958663498339 70-71 2.3555172810169803E-5 72-73 0.0 74-75 0.0 76-77 0.08998076013484865 78-79 0.0038159379952475087 80-81 2.826620737220377E-4 82-83 0.0 84-85 7.066551843050942E-5 86-87 0.09217139120619444 88-89 0.43077700035238536 90-91 0.3863048340867848 92-93 0.0 94-95 0.0010128724308373015 96-97 0.0 98-99 0.0 100-101 4.2399311058305644E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2122676.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.57516700474711 #Duplication Level Percentage of deduplicated Percentage of total 1 70.06734485199297 29.831289086528084 2 14.900568935832439 12.68788421817626 3 5.783210042883192 7.386634001978478 4 2.8558446732204374 4.863522556079102 5 1.5957000202213765 3.3968597425201725 6 1.0086578642191908 2.576626621187015 7 0.6742947706452396 2.0095748729454104 8 0.4959258698807885 1.6891301385719246 9 0.3807890336798422 1.4590941032245996 >10 1.9948004054987138 15.487266522472378 >50 0.15507182021918786 4.53041420828149 >100 0.07937986338926734 5.909232393539321 >500 0.0035418308705105097 0.9886905532281727 >1k 0.003984559729324323 3.9473638802852262 >5k 7.747755029241738E-4 2.5252347059907208 >10k+ 1.106822147034534E-4 0.711182394991639 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15092 0.7109893361021653 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9839 0.4635186905585214 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8878 0.4182456484173751 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8652 0.40759871030717826 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7595 0.35780307498647934 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7520 0.35426979906495387 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5720 0.26947117694834255 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5384 0.25364210081990846 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4957 0.23352598324002344 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4731 0.2228790451298267 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4250 0.20021896888644336 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3679 0.17331896153722942 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3583 0.1687963683576768 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3551 0.16728883729782595 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3449 0.16248358204455132 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3354 0.15800809921061906 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3344 0.15753699575441565 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3221 0.15174242324311388 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3081 0.14514697485626635 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2769 0.13044854702272038 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2730 0.12861124354352713 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2481 0.11688076748406256 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2304 0.10854223630926245 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.711034562033961E-5 0.0 0.0 0.0 0.0 6 4.711034562033961E-5 0.0 0.0 0.0 4.711034562033961E-5 7 9.422069124067921E-5 0.0 0.0 0.0 4.711034562033961E-5 8 1.4133103686101881E-4 0.0 0.0 0.0 4.711034562033961E-5 9 1.4133103686101881E-4 0.0 0.0 0.0 4.711034562033961E-5 10-11 1.4133103686101881E-4 0.0 0.0 0.0 4.711034562033961E-5 12-13 1.648862096711886E-4 0.0 0.0 0.0 4.711034562033961E-5 14-15 2.3555172810169804E-4 0.0 0.0 2.3555172810169803E-5 4.711034562033961E-5 16-17 2.8266207372203763E-4 0.0 0.0 1.4133103686101881E-4 4.711034562033961E-5 18-19 3.062172465322074E-4 0.0 0.0 1.8844138248135843E-4 4.711034562033961E-5 20-21 3.7688276496271686E-4 0.0 0.0 2.3555172810169804E-4 4.711034562033961E-5 22-23 3.7688276496271686E-4 0.0 0.0 6.595448386847545E-4 4.711034562033961E-5 24-25 5.653241474440753E-4 0.0 0.0 0.001319089677369509 4.711034562033961E-5 26-27 5.653241474440753E-4 0.0 0.0 0.0020728552072949427 4.711034562033961E-5 28-29 6.12434493064415E-4 0.0 0.0 0.006053679412213639 4.711034562033961E-5 30-31 6.12434493064415E-4 0.0 0.0 0.0167712830408409 4.711034562033961E-5 32-33 6.359896658745847E-4 0.0 0.0 0.03905447651926154 4.711034562033961E-5 34-35 7.066551843050941E-4 0.0 0.0 0.07250282190970266 4.711034562033961E-5 36-37 8.008758755457734E-4 0.0 0.0 0.1234055503524796 9.422069124067921E-5 38-39 8.008758755457734E-4 0.0 0.0 0.19383551705488733 1.1777586405084902E-4 40-41 8.008758755457734E-4 0.0 0.0 0.2685760803815561 1.4133103686101881E-4 42-43 8.950965667864526E-4 0.0 0.0 0.34932321277481815 1.648862096711886E-4 44-45 9.893172580271318E-4 0.0 0.0 0.4444625557550941 1.8844138248135843E-4 46-47 0.0011542034676983205 4.711034562033961E-5 0.0 0.5641934991491871 2.5910690091186783E-4 48-49 0.0011777586405084903 4.711034562033961E-5 0.0 0.691791870261877 3.2977241934237727E-4 50-51 0.0011777586405084903 4.711034562033961E-5 0.0 0.8284825380792924 3.2977241934237727E-4 52-53 0.0011777586405084903 4.711034562033961E-5 0.0 0.9634301231087552 3.768827649627169E-4 54-55 0.0011777586405084903 4.711034562033961E-5 0.0 1.1165151912020486 4.239931105830565E-4 56-57 0.00122486898612883 4.711034562033961E-5 0.0 1.301753070181224 4.239931105830565E-4 58-59 0.00122486898612883 4.711034562033961E-5 0.0 1.514409170311437 4.239931105830565E-4 60-61 0.00122486898612883 4.711034562033961E-5 0.0 1.7511857673992641 4.239931105830565E-4 62-63 0.00122486898612883 4.711034562033961E-5 0.0 1.9900587748671958 4.239931105830565E-4 64-65 0.00122486898612883 4.711034562033961E-5 0.0 2.2291437788904194 4.475482833932263E-4 66-67 0.0012719793317491695 4.711034562033961E-5 0.0 2.5068828214951315 4.711034562033961E-4 68-69 0.0012719793317491695 4.711034562033961E-5 0.0 2.827233171713441 4.711034562033961E-4 70-71 0.0012719793317491695 4.711034562033961E-5 0.0 3.1747661913546863 4.711034562033961E-4 72-73 0.0012719793317491695 4.711034562033961E-5 0.0 3.5340767974010165 5.653241474440753E-4 74-75 0.0012955345045593393 4.711034562033961E-5 0.0 3.9226193729047676 6.12434493064415E-4 76-77 0.001319089677369509 4.711034562033961E-5 0.0 4.315236993304678 6.12434493064415E-4 78-79 0.001319089677369509 4.711034562033961E-5 0.0 4.78440892533764 6.12434493064415E-4 80-81 0.0014133103686101882 4.711034562033961E-5 0.0 5.296521937403542 6.12434493064415E-4 82-83 0.0014133103686101882 4.711034562033961E-5 0.0 5.800861742442088 6.359896658745847E-4 84-85 0.001436865541420358 4.711034562033961E-5 0.0 6.360673979448583 6.595448386847545E-4 86-87 0.0014604207142305278 4.711034562033961E-5 0.0 6.954193668746432 6.595448386847545E-4 88-89 0.0014604207142305278 4.711034562033961E-5 0.0 7.55873717891944 6.595448386847545E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 500 0.0 29.42231 3 GTAAGCG 375 0.0 25.31011 94-95 GTACATG 10160 0.0 22.597897 1 TATCCGG 105 0.002208872 22.59778 5 TACATGG 10050 0.0 22.334703 2 GAGTACT 8855 0.0 22.296719 12-13 AGTACTT 9445 0.0 20.77829 12-13 GTACTTT 9410 0.0 20.72948 14-15 ACATGGG 10115 0.0 20.68987 3 CTTTGCG 715 0.0 20.57504 2 GTCGTAA 190 8.058427E-6 20.056313 1 GTACCGT 145 6.1874604E-4 19.636692 6 CGTATAG 270 2.228444E-8 19.40634 1 CATGGGA 6110 0.0 18.951067 4 CATGGGG 3670 0.0 18.102856 4 ACTTTTT 10915 0.0 17.91323 16-17 TACTTTT 10895 0.0 17.620876 14-15 CTATACA 945 0.0 17.576052 4 CCCTATA 570 0.0 17.483547 2 AGTACAT 8020 0.0 16.56795 2 >>END_MODULE