FastQCFastQC Report
Thu 26 May 2016
SRR938912_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938912_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129368
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT145990.6856024886257331No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103030.4838524858079956No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA90700.4259479808093294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81500.3827426729433334No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT78680.3694993068365825No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA74390.34935248392950397No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61740.2899451856137596No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC52610.2470686137858745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52260.2454249335953203No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT50250.23598551307242335No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41470.19475262143509248No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT34790.16338181094108675No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33880.15910824244564584No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA33430.15699493934350472No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33040.1551634099883158No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG31040.14577095175657753No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG30540.143422837198643No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30020.14098079805839103No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT29920.1405111751468041No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28110.13201100044708103No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC24970.11726484102325196No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG24130.1133200085659219No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA22360.10500768303083356No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG1904.0563464E-1027.4496881
GTATAGG6750.025.2869871
GTACATG105850.023.2474231
TTTGCGT6750.023.1366963
TACATGG105750.022.778682
GAGTACT81100.022.70022212-13
ACATGGG107050.021.7063083
GTACTTT86200.021.24786614-15
CATGGGG40450.020.2403834
AGTACTT87400.019.89973412-13
CATGGGA62350.018.5960544
ACTTTTT103200.018.02479716-17
ATGGGGA21650.017.9245035
TACCTGG12400.017.1744172
TACTTTT100200.017.0274314-15
CTTTGCG9050.016.7337232
CCCTATA6000.016.5637722
AGTACAT81500.016.5492532
ATGGGAT26750.016.2762835
CCGTCTA1850.003222224315.349039
Produced by FastQC (version 0.11.5)