##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938912_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2129368 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.867538161557796 33.0 31.0 34.0 30.0 34.0 2 31.923732299912462 33.0 31.0 34.0 30.0 34.0 3 31.967344301219892 34.0 31.0 34.0 30.0 34.0 4 35.50793427909126 37.0 35.0 37.0 33.0 37.0 5 35.550874719635125 37.0 35.0 37.0 33.0 37.0 6 35.64863518189435 37.0 35.0 37.0 33.0 37.0 7 35.56960844720123 37.0 35.0 37.0 33.0 37.0 8 35.557486070984446 37.0 35.0 37.0 33.0 37.0 9 37.16414260005786 39.0 37.0 39.0 33.0 39.0 10-11 37.05286169417405 39.0 37.0 39.0 33.0 39.0 12-13 36.93383318430633 39.0 37.0 39.0 33.0 39.0 14-15 38.10663797896841 40.0 38.0 41.0 33.0 41.0 16-17 38.047981607688286 40.0 38.0 41.0 33.0 41.0 18-19 38.028964697506495 40.0 38.0 41.0 33.0 41.0 20-21 37.9080424332478 40.0 38.0 41.0 33.0 41.0 22-23 37.99715103260686 40.0 38.0 41.0 33.0 41.0 24-25 37.92634645584981 40.0 38.0 41.0 33.0 41.0 26-27 37.821307542895354 40.0 38.0 41.0 32.5 41.0 28-29 37.69730314346792 40.0 37.5 41.0 32.0 41.0 30-31 37.518921341919295 40.0 37.0 41.0 32.0 41.0 32-33 37.31689449639518 40.0 36.5 41.0 31.5 41.0 34-35 36.98976644713361 40.0 36.0 41.0 30.5 41.0 36-37 36.702858547700544 39.5 36.0 41.0 30.0 41.0 38-39 36.4819599054743 39.0 35.5 41.0 30.0 41.0 40-41 36.33326249854417 39.0 35.0 41.0 30.0 41.0 42-43 36.12563962640559 39.0 35.0 41.0 29.5 41.0 44-45 36.03742002321816 39.0 35.0 41.0 29.0 41.0 46-47 35.7723362049209 39.0 35.0 41.0 27.5 41.0 48-49 35.54871468905328 39.0 35.0 41.0 26.5 41.0 50-51 34.36820666977244 38.0 34.0 40.0 24.5 40.5 52-53 34.51418871702778 38.0 34.0 39.5 25.0 40.5 54-55 35.01735256658314 39.0 35.0 40.0 26.0 41.0 56-57 34.915113545427566 39.0 35.0 40.5 25.5 41.0 58-59 34.71548482930147 38.0 34.0 40.0 25.0 41.0 60-61 34.41979756434773 38.0 34.0 40.0 24.0 41.0 62-63 34.055402588937184 37.5 34.0 40.0 23.0 41.0 64-65 33.62405370983315 37.0 33.5 40.0 22.0 41.0 66-67 33.21496026050922 36.0 33.0 39.0 21.5 41.0 68-69 32.815924959894204 36.0 32.5 39.0 20.0 41.0 70-71 32.35454956588058 35.0 32.0 38.5 18.5 40.0 72-73 31.894934318539583 35.0 31.5 37.5 17.5 40.0 74-75 31.431100918206717 35.0 31.0 37.0 13.5 39.0 76-77 31.00548707409898 35.0 31.0 36.5 9.5 39.0 78-79 30.572158264799697 35.0 31.0 36.0 7.0 38.5 80-81 30.128661180218728 34.0 30.0 35.5 4.0 37.0 82-83 29.737568142284474 34.0 30.0 35.0 2.0 37.0 84-85 29.376238395617854 34.0 29.5 35.0 2.0 36.5 86-87 28.990698413801653 34.0 29.0 35.0 2.0 36.0 88-89 28.54284557671572 34.0 29.0 35.0 2.0 36.0 90-91 28.080144906845597 34.0 28.5 35.0 2.0 35.0 92-93 27.668356291632072 34.0 27.0 35.0 2.0 35.0 94-95 27.178118577906684 33.5 26.0 35.0 2.0 35.0 96-97 26.560663774415694 33.0 25.0 35.0 2.0 35.0 98-99 25.82898611231126 33.0 23.5 35.0 2.0 35.0 100-101 23.112460129014806 29.5 9.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3438958413920403 1101 2 0.35607675304931874 1101 3 0.30372756384260313 1101 4 0.20105254468426637 1101 5 0.10079072797483235 1101 6 0.302447819230764 1101 7 0.2844476900497952 1101 8 0.3897734360538294 1101 9 0.3298376723947456 1101 10-11 0.4317430900309347 1101 12-13 0.5698370664339194 1101 14-15 0.32316283155874714 1101 16-17 0.5266460481460271 1101 18-19 0.6388253204646333 1101 20-21 0.7525255058652434 1101 22-23 0.6482894519992257 1101 24-25 0.6989690986406387 1101 26-27 0.7126809124777509 1101 28-29 0.616193307573333 1101 30-31 0.7237545948724033 1101 32-33 0.7618661470743362 1101 34-35 0.6552990122816453 1101 36-37 0.6040094544422985 1101 38-39 0.6303067992599338 1101 40-41 0.7009827361974104 1101 42-43 0.6104813252627537 1101 44-45 0.6428113881586555 1101 46-47 0.5647965530175085 1101 48-49 0.6192401015107762 1101 50-51 0.6422519569875291 1101 52-53 0.6216007970635289 1101 54-55 0.47305423622543685 1101 56-57 0.5755508295507497 1101 58-59 0.4151264541808146 1101 60-61 0.5406587881412577 1101 62-63 0.5487765007340428 1101 64-65 0.5569938009193507 1101 66-67 0.5086790286014491 1101 68-69 0.5841341642892388 1101 70-71 0.4776546880909365 1101 72-73 0.2669393070599817 1101 74-75 0.27337125694063147 1101 76-77 0.16092226541623234 1101 78-79 0.29263355385733547 1101 80-81 0.25295929777244197 1101 82-83 0.18556485936933953 1101 84-85 0.08469025712717482 1101 86-87 0.11567670242444805 1101 88-89 0.0977185574570072 1101 90-91 0.03907575607856728 1101 92-93 0.08447712146010389 1101 94-95 0.24510392662868696 1101 96-97 0.1276290142043912 1101 98-99 0.25568754610932487 1101 100-101 0.6640884001314937 1102 1 0.2224681732079219 1102 2 0.2635958115072974 1102 3 0.19550081335485103 1102 4 0.2059089890323591 1102 5 0.3008872245906744 1102 6 0.3022679230436651 1102 7 0.32410614321355524 1102 8 0.21045758351149857 1102 9 0.34990249523840333 1102 10-11 0.36464821022266136 1102 12-13 0.41438192155350606 1102 14-15 0.605828667240516 1102 16-17 0.4320925369337445 1102 18-19 0.46430080339933255 1102 20-21 0.5863322898332584 1102 22-23 0.5181970350375025 1102 24-25 0.5755648305095065 1102 26-27 0.5618014534609657 1102 28-29 0.6709250289164004 1102 30-31 0.5306819529425866 1102 32-33 0.5367080040245042 1102 34-35 0.5946412546161071 1102 36-37 0.6460498385018454 1102 38-39 0.6345622096942165 1102 40-41 0.6869625005108162 1102 42-43 0.6023615339724415 1102 44-45 0.7986067551167721 1102 46-47 0.7344789271052221 1102 48-49 0.655179821986728 1102 50-51 0.6809379169557346 1102 52-53 0.7195964223495537 1102 54-55 0.7552737186061549 1102 56-57 0.5932171620169697 1102 58-59 0.6427835735536007 1102 60-61 0.5264857536011931 1102 62-63 0.564635967940248 1102 64-65 0.6105949612873829 1102 66-67 0.5820778019954744 1102 68-69 0.3801141995759849 1102 70-71 0.5351439394819337 1102 72-73 0.5287838526678037 1102 74-75 0.572505120779546 1102 76-77 0.5718962339110618 1102 78-79 0.5049291195292298 1102 80-81 0.3574481216277263 1102 82-83 0.37723145076563114 1102 84-85 0.40721024893776203 1102 86-87 0.36080256953912837 1102 88-89 0.4033196515871218 1102 90-91 0.32042661837593656 1102 92-93 0.2331816431018403 1102 94-95 0.31054850354898633 1102 96-97 0.17478176187402283 1102 98-99 0.3008339620353304 1102 100-101 0.4578366689129574 1103 1 0.010806437507728361 1103 2 0.18055305298377178 1103 3 0.2407952624954497 1103 4 0.2474582517354733 1103 5 0.21199187848379353 1103 6 0.2038980770776604 1103 7 0.2240310046768812 1103 8 0.13778159497012865 1103 9 0.25115695658901416 1103 10-11 0.26547561465583414 1103 12-13 0.3027395575073015 1103 14-15 0.22056169062738462 1103 16-17 0.2819548807864791 1103 18-19 0.3265698111010309 1103 20-21 0.4414469935056573 1103 22-23 0.43115520400528595 1103 24-25 0.5667125716572556 1103 26-27 0.39858059312471994 1103 28-29 0.4062011043367022 1103 30-31 0.4176938459640951 1103 32-33 0.4138491588098887 1103 34-35 0.38613034778789057 1103 36-37 0.2771739227509258 1103 38-39 0.38997306486468375 1103 40-41 0.3135225056765947 1103 42-43 0.2218156642438771 1103 44-45 0.3461348146640617 1103 46-47 0.2280818786408716 1103 48-49 0.14741208224783264 1103 50-51 0.2726891144761652 1103 52-53 0.14654033847616432 1103 54-55 0.37376073372778507 1103 56-57 0.3423146190471229 1103 58-59 0.29222267611771 1103 60-61 0.31467873049609807 1103 62-63 0.28328323947213363 1103 64-65 0.1270711400231832 1103 66-67 0.09647006037811678 1103 68-69 0.08200352285953727 1103 70-71 0.06405173040895562 1103 72-73 0.12206130171448493 1103 74-75 0.18574858137877115 1103 76-77 0.18790602540162382 1103 78-79 0.08127691703087336 1103 80-81 0.17370504846157786 1103 82-83 0.09522566275023081 1103 84-85 0.07306095805769885 1103 86-87 0.1110094940248949 1103 88-89 0.21595026349658397 1103 90-91 -0.003294265441098787 1103 92-93 0.11455227567439508 1103 94-95 0.19909105074538047 1103 96-97 -0.053279839985656 1103 98-99 -0.09254269336055998 1103 100-101 0.14299299229043427 1104 1 0.1702684808006083 1104 2 0.09475734970198246 1104 3 0.16845925365180037 1104 4 0.17516032077762134 1104 5 0.2669698795241118 1104 6 0.24389638153407134 1104 7 0.2127676139537087 1104 8 0.1640095948130238 1104 9 0.18797089606481876 1104 10-11 0.20080463632718448 1104 12-13 0.19859510294068627 1104 14-15 0.3158286094223399 1104 16-17 0.17302243143485185 1104 18-19 0.2660778208681265 1104 20-21 0.36842402607445734 1104 22-23 0.26036977935783057 1104 24-25 0.29017336293008356 1104 26-27 0.343025676191985 1104 28-29 0.38320505485748413 1104 30-31 0.5170420435629381 1104 32-33 0.5417151782961867 1104 34-35 0.3846491419785991 1104 36-37 0.21457196262590372 1104 38-39 0.3391456731184377 1104 40-41 0.4443705744332789 1104 42-43 0.4568369255942031 1104 44-45 0.5227299906988421 1104 46-47 0.6569190997502901 1104 48-49 0.6365470724242144 1104 50-51 0.6421679407768224 1104 52-53 0.5424156413031227 1104 54-55 0.6851968878900578 1104 56-57 0.6875912369261954 1104 58-59 0.6455028345217073 1104 60-61 0.6647235857666018 1104 62-63 0.586164082990706 1104 64-65 0.6231936617275764 1104 66-67 0.7303723826980857 1104 68-69 0.5822979678947178 1104 70-71 0.43729607644161916 1104 72-73 0.6066803504283165 1104 74-75 0.3781260831437656 1104 76-77 0.42599279604247187 1104 78-79 0.6260167564619366 1104 80-81 0.482523767071271 1104 82-83 0.38546632456783314 1104 84-85 0.5098832794580481 1104 86-87 0.3725009928375371 1104 88-89 0.46468195857078953 1104 90-91 0.588305202139054 1104 92-93 0.6264771199861343 1104 94-95 0.5285533718391875 1104 96-97 0.48523246899028294 1104 98-99 0.5362225868878916 1104 100-101 0.9468633818727064 1105 1 0.0862594214145247 1105 2 0.028747908931634925 1105 3 0.12118572146443185 1105 4 0.09562056471954605 1105 5 0.047779375760086396 1105 6 0.20813219964861673 1105 7 0.21819873960170355 1105 8 0.20487740428533385 1105 9 0.153298142115041 1105 10-11 0.08080667564134814 1105 12-13 0.242661709078277 1105 14-15 0.15095830446125547 1105 16-17 0.17390516645709653 1105 18-19 0.23977171330800928 1105 20-21 0.21447856866999615 1105 22-23 0.12974404205169066 1105 24-25 0.2284450883036797 1105 26-27 0.043951427271629484 1105 28-29 0.23937278403469975 1105 30-31 0.2532432843857322 1105 32-33 0.1727142866188487 1105 34-35 0.14748642723800032 1105 36-37 0.14766283125992175 1105 38-39 0.11846110461804216 1105 40-41 0.2255914175247824 1105 42-43 0.2031296359286756 1105 44-45 0.043546092640390555 1105 46-47 0.21584784662425704 1105 48-49 0.20744854451638872 1105 50-51 0.03827719413002484 1105 52-53 0.03296999332265926 1105 54-55 0.10621724642378183 1105 56-57 0.0448839567836643 1105 58-59 0.06041419287450367 1105 60-61 0.05749770106311303 1105 62-63 -0.05176567401734644 1105 64-65 -0.030146040397845297 1105 66-67 -0.047203941189600584 1105 68-69 0.023560958360803852 1105 70-71 -0.2080261944252868 1105 72-73 -0.003656765856025146 1105 74-75 0.11380634655440502 1105 76-77 0.05625735640884244 1105 78-79 0.048548599841680584 1105 80-81 0.03376641317198903 1105 82-83 -0.08632587257020319 1105 84-85 -0.0028051799980417513 1105 86-87 0.1663371309246564 1105 88-89 0.2936678132094954 1105 90-91 0.2389884536791662 1105 92-93 0.3547731150564779 1105 94-95 0.27696494817561756 1105 96-97 0.14594110975951935 1105 98-99 -0.04806565878861235 1105 100-101 0.43140500885055744 1106 1 0.06933367168768001 1106 2 0.10014894572305977 1106 3 0.11822559217660356 1106 4 5.380344415328864E-4 1106 5 0.06690002473393974 1106 6 0.00789677604109329 1106 7 0.014384206751344664 1106 8 0.04031345568914446 1106 9 0.12814106102797496 1106 10-11 -0.013939387602512454 1106 12-13 0.11139272899698938 1106 14-15 0.20988388750432563 1106 16-17 0.0948944185681384 1106 18-19 0.13987544296070809 1106 20-21 0.19347205124270062 1106 22-23 0.12385810747576187 1106 24-25 0.15980592813274797 1106 26-27 0.15662472042177455 1106 28-29 0.08756657251575461 1106 30-31 0.12604232965048823 1106 32-33 0.183368731645686 1106 34-35 0.15316549944765967 1106 36-37 0.20962465332936375 1106 38-39 0.12264851778922292 1106 40-41 0.19720282943963952 1106 42-43 0.19543440182177108 1106 44-45 0.21206981396809255 1106 46-47 0.266815886733049 1106 48-49 0.23469456815563916 1106 50-51 0.05578932175193785 1106 52-53 0.06326867181422813 1106 54-55 -0.08975982009337002 1106 56-57 -0.06249310876921044 1106 58-59 0.22114361115961856 1106 60-61 0.19933376642610767 1106 62-63 0.0990381389542776 1106 64-65 0.06223591431606934 1106 66-67 0.08146020317966673 1106 68-69 0.1653563859448539 1106 70-71 0.2700365222951788 1106 72-73 0.08409936981721344 1106 74-75 0.07566836499385232 1106 76-77 0.1394048924392841 1106 78-79 0.1477453034519911 1106 80-81 0.07145140977288023 1106 82-83 0.05420702876287109 1106 84-85 0.19482150483394278 1106 86-87 0.08400292445305624 1106 88-89 0.035040607892515396 1106 90-91 -0.024484519787119297 1106 92-93 -0.0017513033124423316 1106 94-95 0.13234427658695935 1106 96-97 0.41539633163375456 1106 98-99 0.44155862083787767 1106 100-101 0.855772362433445 1107 1 0.025178297372203673 1107 2 0.041690537936553085 1107 3 0.14300388335075453 1107 4 0.12147841642127588 1107 5 0.027812509986596012 1107 6 0.11759203501892301 1107 7 0.03748222908247811 1107 8 0.14024990397359005 1107 9 0.1131958617731712 1107 10-11 0.0464566367929109 1107 12-13 0.19199909306394858 1107 14-15 0.22099615257275218 1107 16-17 0.27551919245746603 1107 18-19 0.2646494469159606 1107 20-21 0.32504863358183655 1107 22-23 0.20330428591302052 1107 24-25 0.29717873872800027 1107 26-27 0.3241809276957426 1107 28-29 0.30770116367784794 1107 30-31 0.3129602594418799 1107 32-33 0.3137971646648836 1107 34-35 0.30675167782072776 1107 36-37 0.2872760460335684 1107 38-39 0.3579747659774739 1107 40-41 0.3663149303557276 1107 42-43 0.469550810590448 1107 44-45 0.4311353515601226 1107 46-47 0.4541179467532075 1107 48-49 0.6124891127039689 1107 50-51 0.556756582530852 1107 52-53 0.6398326699049264 1107 54-55 0.6816805158045014 1107 56-57 0.6996415548400066 1107 58-59 0.7639183634294398 1107 60-61 0.55010553460815 1107 62-63 0.500265544508224 1107 64-65 0.6105658247521646 1107 66-67 0.49359582969366755 1107 68-69 0.4084356224622354 1107 70-71 0.45601905887065897 1107 72-73 0.5007672974306168 1107 74-75 0.4811848235584577 1107 76-77 0.5358365736572779 1107 78-79 0.5695980147855195 1107 80-81 0.37781527039745555 1107 82-83 0.5443038969838518 1107 84-85 0.7031720672849637 1107 86-87 0.7448654532886145 1107 88-89 1.0102187567401693 1107 90-91 0.7580872246432797 1107 92-93 0.7357975354473183 1107 94-95 0.7798741277814898 1107 96-97 0.747342394293181 1107 98-99 0.7089089040465169 1107 100-101 0.7894893216168306 1108 1 0.13349013956428735 1108 2 0.15922426744626605 1108 3 0.16097642055020245 1108 4 0.21805775675437644 1108 5 0.16153584260394638 1108 6 0.2175320423284859 1108 7 0.22816422243086976 1108 8 0.12542277136681435 1108 9 0.24650551624429085 1108 10-11 0.25187462720624154 1108 12-13 0.2802863859850433 1108 14-15 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sequence quality scores pass #Quality Count 2 6229.0 3 1451.0 4 1413.0 5 1703.0 6 2043.0 7 2454.0 8 3140.0 9 3933.0 10 4837.0 11 6604.0 12 8844.0 13 13327.0 14 12748.0 15 12161.0 16 15600.0 17 20944.0 18 18255.0 19 12820.0 20 12685.0 21 13635.0 22 14791.0 23 16453.0 24 18604.0 25 21239.0 26 24934.0 27 28927.0 28 34642.0 29 41174.0 30 49584.0 31 60886.0 32 76158.0 33 97380.0 34 132285.0 35 188288.0 36 286913.0 37 431298.0 38 374138.0 39 56841.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.449075552365585 19.0683749332414 14.451835378713168 22.030714135679847 2 15.045572632072734 21.36266188731003 40.05188290576235 23.539882574854886 3 20.332209422518545 25.678330237995517 30.188169275728555 23.80129106375738 4 12.566828075783931 16.566451976869917 38.28306144516008 32.583658502186076 5 13.042901838918885 38.4637987085491 34.24610907518106 14.247190377350952 6 30.194196158942205 36.506405601452535 17.747395998573033 15.552002241032229 7 27.066967257192925 33.92887361283991 21.420600479089654 17.583558650877514 8 25.236375977290987 33.77002468228201 20.443049547413157 20.550549793013847 9 26.037523162881477 17.815417101093107 21.60439913769681 34.54266059832861 10-11 24.655204992159796 26.256171574470642 27.435609749677553 21.653013683692002 12-13 26.94362222845837 24.155945737975472 27.443321154038696 21.45711087952746 14-15 23.543467790898042 25.375640248467295 27.247888834226565 23.8330031264081 16-17 21.212464490697837 28.497785244253 28.050016087444252 22.239734177604912 18-19 21.10845844068265 28.094193017932202 29.75500968053642 21.042338860848723 20-21 22.60050654334119 26.345401444412452 29.104523044196203 21.94956896805016 22-23 21.915605519664847 26.330848423142644 29.23504557915036 22.51850047804215 24-25 22.51892127512854 26.553218562328706 29.2839810019606 21.64387916058215 26-27 22.189752361471136 25.83331276046718 30.30994492349833 21.666989954563356 28-29 21.5152694924714 27.062024481405256 30.00911716759366 21.41358885852968 30-31 21.733310577220095 26.78481509012408 29.39901763175625 22.082856700899573 32-33 21.659447219206122 26.74656601204649 29.511057661002454 22.082929107744935 34-35 22.48459116739409 26.30646251672413 29.26987543760896 21.939070878272815 36-37 22.503599725627215 25.928889829499607 29.92193980805243 21.64557063682075 38-39 22.2905044556637 26.44189736127032 29.825504807544707 21.442093375521267 40-41 21.878046080042456 26.75304894242816 29.85091750970319 21.517987467826195 42-43 21.36202805683909 27.08711939458283 28.880956788840738 22.66989575973734 44-45 21.887950585302093 26.75128876997517 28.74515633643073 22.61560430829201 46-47 22.499178734526144 27.063643528038394 28.204015805138916 22.233161932296554 48-49 22.342355238612896 26.914157845665493 28.447548674658897 22.29593824106272 50-51 21.9930922461254 27.25518229162361 28.981908184930155 21.769817277320833 52-53 22.14627403516828 27.714624096629148 28.219491362881794 21.919610505320783 54-55 21.58162630263658 27.065837602140192 28.62317959172973 22.7293565034935 56-57 22.25611246207713 27.482308562842118 27.788713794563286 22.472865180517474 58-59 22.62074332315034 26.90005626263578 28.104142776295465 22.37505763791842 60-61 22.084818995075192 26.915282474343577 28.999629518633796 22.000269011947434 62-63 22.25841943323433 26.836082209100965 28.83482161728404 22.070676740380666 64-65 21.895828062275147 27.546378815507882 28.58393568092386 21.97385744129311 66-67 21.818396177378414 27.228594623304307 28.047947212136222 22.905061987181064 68-69 21.484073659546105 27.42107239146856 28.52373516530018 22.571118783685158 70-71 22.359969918750856 27.197045176450906 27.805569094847844 22.637415809950394 72-73 21.984027517744906 26.73705174106199 28.332551758189705 22.9463689830034 74-75 21.62897565234811 28.18182118848594 27.66245154754908 22.52675161161687 76-77 22.12501606149474 26.900122067360023 27.99168959366308 22.98317227748216 78-79 21.98393750693672 26.963924330119415 28.36109202283037 22.691046140113492 80-81 22.279510123508096 27.4711241822503 27.765909592686565 22.48345610155504 82-83 22.08525786932316 27.148037221262083 28.19469038252352 22.57201452689123 84-85 21.94660518189638 27.206538472621183 27.918223743910453 22.92863260157198 86-87 21.963448374400873 27.33946817467412 28.058196366092503 22.638887084832504 88-89 22.25465480985011 26.94216625068291 27.738601741864578 23.064577197602404 90-91 22.021804946082934 26.63870179496774 28.164296810332118 23.175196448617204 92-93 22.254614387287237 27.412158433281803 27.84753905644616 22.485688122984808 94-95 22.14351002785114 27.205547264604245 27.715367927271885 22.935574780272727 96-97 21.49781929462414 26.92432898011214 28.092143838202105 23.485707887061622 98-99 22.088761200161688 27.216638006002086 27.33743095394299 23.357169839893235 100-101 22.38921812002069 26.349919811421024 28.039194382311745 23.221667686246537 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3264.0 1 1949.5 2 721.0 3 819.0 4 734.5 5 750.0 6 1195.5 7 2256.5 8 3542.5 9 4123.5 10 3729.0 11 3339.5 12 3412.0 13 3604.5 14 3810.0 15 4092.0 16 4459.0 17 4552.0 18 4564.0 19 4721.5 20 5067.5 21 5588.5 22 6358.5 23 7626.0 24 8739.0 25 9957.5 26 12270.5 27 15171.0 28 17487.0 29 19747.0 30 22352.5 31 26030.0 32 30839.5 33 35359.5 34 39705.0 35 44147.5 36 50938.0 37 56842.5 38 58813.5 39 59911.5 40 60106.0 41 60293.5 42 60410.5 43 62099.0 44 65463.0 45 67983.5 46 72088.5 47 86497.5 48 126764.5 49 125718.0 50 85279.0 51 71636.5 52 67445.5 53 65675.0 54 63763.5 55 59583.5 56 54584.0 57 49800.5 58 46450.0 59 42149.5 60 34951.0 61 28327.0 62 22603.0 63 16914.0 64 11750.5 65 8082.5 66 5759.0 67 4565.0 68 3609.0 69 2627.0 70 1901.5 71 1235.5 72 784.5 73 534.5 74 345.5 75 196.0 76 124.0 77 75.0 78 53.0 79 37.0 80 24.0 81 24.5 82 21.5 83 14.5 84 10.5 85 7.5 86 6.5 87 5.0 88 3.0 89 3.5 90 4.5 91 4.0 92 4.0 93 2.5 94 2.0 95 1.5 96 1.5 97 1.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28393401234544713 2 0.08852391883413294 3 0.09275052503841516 4 0.10651047634791168 5 0.10815415653846587 6 0.0834519913889943 7 0.09392458231738243 8 0.09068418422743274 9 0.09589699854604747 10-11 0.08791340904906997 12-13 0.09331407253231945 14-15 0.10174380379530452 16-17 0.09075462766417078 18-19 0.10150899233951106 20-21 0.10357533315049347 22-23 0.11569160426943581 24-25 0.1280896491353303 26-27 0.13057865056674095 28-29 0.12261854221534277 30-31 0.11585597228849123 32-33 0.11076056369777323 34-35 0.38424546626041156 36-37 0.5889071311299878 38-39 0.5717658948570655 40-41 0.5344073922403267 42-43 0.5612933039286774 44-45 0.5836003922290558 46-47 0.5733391316108817 48-49 0.5957871067847361 50-51 0.5856902141856175 52-53 0.5905038490293834 54-55 0.5806182867404789 56-57 0.5516895153867251 58-59 0.54577226670073 60-61 0.49324494403973385 62-63 0.5686429024950126 64-65 0.6038176585728724 66-67 0.6163331091666635 68-69 0.6186107802878601 70-71 0.6151825330332755 72-73 0.6065414714600764 74-75 0.603935064300769 76-77 0.587216488648275 78-79 0.5641580036893576 80-81 0.5586399344782114 82-83 0.5725172915156046 84-85 0.5833186184821035 86-87 0.5741374905605795 88-89 0.5869581960469021 90-91 0.6003659301727085 92-93 0.6078798967580991 94-95 0.6075746418655676 96-97 0.6035124036803409 98-99 0.607527679574409 100-101 0.5862302805339424 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2129368.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.16827220101163 #Duplication Level Percentage of deduplicated Percentage of total 1 71.73511927995384 31.684162747290383 2 14.24205915912543 12.580942912863257 3 5.349667309543336 7.088566857382908 4 2.6894500930050795 4.751534551155376 5 1.4723075753518398 3.2514640875875744 6 0.9815754310609995 2.601269449495654 7 0.6538095062171654 2.021434536674614 8 0.4513605604661828 1.5948652876377225 9 0.3399181945281496 1.351223940779616 >10 1.8587847063486014 15.037067916059168 >50 0.1443628519943116 4.388459492080304 >100 0.07411209612750158 5.7513270997924835 >500 0.002775773474560078 0.8162405228376681 >1k 0.003629857620578562 3.3508300285018784 >5k 8.540841460184852E-4 2.5563747813170705 >10k+ 2.135210365046213E-4 1.1742357885444024 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14599 0.6856024886257331 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10303 0.4838524858079956 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9070 0.4259479808093294 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8150 0.3827426729433334 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7868 0.3694993068365825 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7439 0.34935248392950397 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6174 0.2899451856137596 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5261 0.2470686137858745 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5226 0.2454249335953203 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5025 0.23598551307242335 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4147 0.19475262143509248 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3479 0.16338181094108675 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3388 0.15910824244564584 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3343 0.15699493934350472 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3304 0.1551634099883158 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3104 0.14577095175657753 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3054 0.143422837198643 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3002 0.14098079805839103 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2992 0.1405111751468041 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2811 0.13201100044708103 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2497 0.11726484102325196 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2413 0.1133200085659219 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2236 0.10500768303083356 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.6962291158691214E-5 7 4.6962291158691214E-5 0.0 0.0 0.0 4.6962291158691214E-5 8 4.6962291158691214E-5 0.0 0.0 0.0 4.6962291158691214E-5 9 4.6962291158691214E-5 0.0 0.0 0.0 4.6962291158691214E-5 10-11 4.6962291158691214E-5 0.0 0.0 0.0 4.6962291158691214E-5 12-13 9.392458231738243E-5 0.0 0.0 0.0 4.6962291158691214E-5 14-15 9.392458231738243E-5 0.0 0.0 0.0 7.044343673803682E-5 16-17 9.392458231738243E-5 0.0 0.0 2.3481145579345607E-5 9.392458231738243E-5 18-19 1.1740572789672803E-4 0.0 0.0 4.6962291158691214E-5 1.4088687347607365E-4 20-21 1.4088687347607365E-4 0.0 0.0 2.582926013728017E-4 1.4088687347607365E-4 22-23 1.4088687347607365E-4 0.0 0.0 6.339909306423314E-4 1.8784916463476488E-4 24-25 1.8784916463476486E-4 0.0 0.0 0.0013619064436020453 2.348114557934561E-4 26-27 1.8784916463476486E-4 0.0 0.0 0.002582926013728017 2.348114557934561E-4 28-29 3.522171836901841E-4 0.0 0.0 0.0065512396166374255 2.348114557934561E-4 30-31 3.756983292695297E-4 0.0 0.0 0.01730560429197771 2.348114557934561E-4 32-33 3.756983292695297E-4 0.0 0.0 0.039730098320252774 3.2873603811083853E-4 34-35 4.4614176600756656E-4 0.0 0.0 0.07265066442249532 3.2873603811083853E-4 36-37 4.696229115869122E-4 0.0 0.0 0.12240721190512865 3.2873603811083853E-4 38-39 5.165852027456034E-4 0.0 0.0 0.19381337561191864 3.2873603811083853E-4 40-41 5.165852027456034E-4 0.0 0.0 0.27125419373260046 3.2873603811083853E-4 42-43 5.635474939042946E-4 0.0 0.0 0.3530859860766199 3.522171836901841E-4 44-45 5.635474939042946E-4 0.0 0.0 0.453373958846005 3.756983292695297E-4 46-47 6.809532218010226E-4 0.0 0.0 0.5745601511810077 4.2266062042822095E-4 48-49 7.044343673803683E-4 0.0 0.0 0.7053970943491215 4.2266062042822095E-4 50-51 7.044343673803683E-4 0.0 0.0 0.8464013735530918 4.2266062042822095E-4 52-53 7.044343673803683E-4 0.0 0.0 0.9878283133774904 4.931040571662578E-4 54-55 7.513966585390594E-4 0.0 0.0 1.1444945166828844 5.635474939042946E-4 56-57 7.983589496977507E-4 0.0 0.0 1.334903126185798 5.635474939042946E-4 58-59 7.983589496977507E-4 0.0 0.0 1.5492155418884852 5.635474939042946E-4 60-61 7.983589496977507E-4 0.0 0.0 1.7968711843138434 5.635474939042946E-4 62-63 7.983589496977507E-4 0.0 0.0 2.0425074482193777 5.635474939042946E-4 64-65 7.983589496977507E-4 0.0 0.0 2.2881202309793327 5.635474939042946E-4 66-67 9.862081143325156E-4 0.0 0.0 2.568884288671568 5.870286394836402E-4 68-69 9.862081143325156E-4 4.6962291158691214E-5 0.0 2.8890497086459455 6.105097850629858E-4 70-71 9.862081143325156E-4 4.6962291158691214E-5 0.0 3.242628798779732 6.105097850629858E-4 72-73 0.0010566515510705525 4.6962291158691214E-5 0.0 3.611353227812196 6.574720762216771E-4 74-75 0.0011270949878085892 4.6962291158691214E-5 0.0 3.999989668295945 6.574720762216771E-4 76-77 0.0011270949878085892 4.6962291158691214E-5 0.0 4.397901161283536 6.574720762216771E-4 78-79 0.0011270949878085892 4.6962291158691214E-5 0.0 4.871187131580826 7.044343673803683E-4 80-81 0.0011740572789672805 4.6962291158691214E-5 0.0 5.3966482073554225 7.044343673803683E-4 82-83 0.0011740572789672805 4.6962291158691214E-5 0.0 5.916520770482133 7.279155129597139E-4 84-85 0.0011740572789672805 4.6962291158691214E-5 0.0 6.476006026201201 7.513966585390594E-4 86-87 0.001197538424546626 4.6962291158691214E-5 0.0 7.07205142558731 7.513966585390594E-4 88-89 0.0012210195701259717 4.6962291158691214E-5 0.0 7.677982387262324 7.983589496977507E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAG 190 4.0563464E-10 27.449688 1 GTATAGG 675 0.0 25.286987 1 GTACATG 10585 0.0 23.247423 1 TTTGCGT 675 0.0 23.136696 3 TACATGG 10575 0.0 22.77868 2 GAGTACT 8110 0.0 22.700222 12-13 ACATGGG 10705 0.0 21.706308 3 GTACTTT 8620 0.0 21.247866 14-15 CATGGGG 4045 0.0 20.240383 4 AGTACTT 8740 0.0 19.899734 12-13 CATGGGA 6235 0.0 18.596054 4 ACTTTTT 10320 0.0 18.024797 16-17 ATGGGGA 2165 0.0 17.924503 5 TACCTGG 1240 0.0 17.174417 2 TACTTTT 10020 0.0 17.02743 14-15 CTTTGCG 905 0.0 16.733723 2 CCCTATA 600 0.0 16.563772 2 AGTACAT 8150 0.0 16.549253 2 ATGGGAT 2675 0.0 16.276283 5 CCGTCTA 185 0.0032222243 15.34903 9 >>END_MODULE