FastQCFastQC Report
Thu 26 May 2016
SRR938912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938912_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129368
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT152100.7142964485236933No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102260.4802363893887764No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA89530.4204533927437625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86350.40551938415529865No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA76010.35696037509721196No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT75590.3549879588685469No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT58310.27383711974632846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54180.254441693497789No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC49910.23438879517302785No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT46850.22001833407846835No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT42240.1983687178543117No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC37680.1769539130859485No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC37050.17399528874295095No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT34910.16394535843499103No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG34770.16328788635876937No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG33720.1583568457871068No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA32810.15408327729166588No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA32100.1507489546193988No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT29890.14037028827332806No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28780.13515747395471334No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC25860.1214444849363755No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG24380.11449406584488919No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA24200.11364874460403275No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG100900.024.0339761
TACATGG100300.023.9387362
ACATGGG102300.022.3574923
GAGTACT87900.021.785512-13
CATGGGG41750.021.3674664
AGTACTT92450.020.94431112-13
GTACTTT93000.020.28462614-15
CATGGGA58400.019.5006924
GTAAGCG3400.018.84111294-95
TAGACGC1801.243609E-418.4534364
TACTTTT107000.017.91886114-15
ATTGCCG1350.00945821217.5746978
ACTTTTT107750.017.50681716-17
ATAGCGT2453.801444E-617.4312326
GGATCGT1952.2942194E-417.033946
GTATTAC4902.7284841E-1115.5568941
AGTACAT76900.015.48813152
ATATAGG8900.015.4617862
CCGTAAA2154.8270117E-415.4493872
TTTGCGT6150.015.4314433
Produced by FastQC (version 0.11.5)