##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938912_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2129368 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1152323130619 33.0 31.0 34.0 30.0 34.0 2 32.344560451739675 34.0 31.0 34.0 30.0 34.0 3 32.43629283430577 34.0 31.0 34.0 30.0 34.0 4 36.015593828779245 37.0 35.0 37.0 35.0 37.0 5 35.93527797919383 37.0 35.0 37.0 35.0 37.0 6 35.98611231125855 37.0 35.0 37.0 35.0 37.0 7 35.92875444732897 37.0 35.0 37.0 35.0 37.0 8 35.90197138305826 37.0 35.0 37.0 35.0 37.0 9 37.5618779844536 39.0 37.0 39.0 35.0 39.0 10-11 37.480848777665486 39.0 37.0 39.0 34.0 39.0 12-13 37.41052861694174 39.0 37.0 39.0 34.0 39.0 14-15 38.60884191929249 40.0 38.0 41.0 34.0 41.0 16-17 38.61058022849973 40.0 38.0 41.0 34.0 41.0 18-19 38.760750842503505 40.0 38.0 41.0 34.0 41.0 20-21 38.76081682452258 40.0 38.0 41.0 34.0 41.0 22-23 38.696565600685275 40.0 38.0 41.0 34.0 41.0 24-25 38.620058392912824 40.0 38.0 41.0 34.0 41.0 26-27 38.434657842139075 40.0 38.0 41.0 34.0 41.0 28-29 38.25141145166077 40.0 38.0 41.0 33.5 41.0 30-31 38.09240629144422 40.0 37.5 41.0 33.0 41.0 32-33 37.91582478932716 40.0 37.0 41.0 33.0 41.0 34-35 37.86790047563409 40.0 37.0 41.0 33.0 41.0 36-37 37.74378900218281 40.0 37.0 41.0 33.0 41.0 38-39 37.59409787317176 40.0 37.0 41.0 33.0 41.0 40-41 37.44022029071537 40.0 37.0 41.0 32.0 41.0 42-43 37.268215029060265 40.0 36.5 41.0 31.5 41.0 44-45 37.00298327954586 40.0 36.0 41.0 31.0 41.0 46-47 36.65206648169786 40.0 36.0 41.0 30.5 41.0 48-49 36.31904043828967 39.0 35.5 41.0 30.0 41.0 50-51 36.032952970083144 39.0 35.0 41.0 29.0 41.0 52-53 35.790914487303276 39.0 35.0 40.0 28.0 41.0 54-55 35.55517904843127 39.0 35.0 40.0 28.0 41.0 56-57 35.27365255794207 38.0 34.5 40.0 27.5 41.0 58-59 34.97235869985836 38.0 34.0 40.0 26.5 41.0 60-61 34.52624722452859 38.0 34.0 40.0 26.0 41.0 62-63 34.19880617159646 37.0 34.0 40.0 26.0 41.0 64-65 33.915164969136384 37.0 33.0 39.5 25.5 41.0 66-67 33.43821828824328 36.0 33.0 39.0 24.0 40.5 68-69 33.007118544093835 35.5 32.5 39.0 23.0 40.0 70-71 32.51370923203504 35.0 32.0 38.0 22.0 40.0 72-73 31.964538539134615 35.0 31.0 37.0 20.5 39.0 74-75 31.501009454448457 35.0 31.0 37.0 19.5 39.0 76-77 29.522649208591467 32.5 28.5 35.0 16.0 37.0 78-79 30.495244833208726 34.0 30.0 36.0 17.5 37.5 80-81 30.504345890423824 34.0 30.0 35.0 17.0 37.0 82-83 30.177689812188405 34.0 30.0 35.0 12.5 37.0 84-85 29.768868978964647 34.0 30.0 35.0 7.5 36.0 86-87 29.212340469096937 34.0 29.0 35.0 2.0 36.0 88-89 28.610990913735908 34.0 29.0 35.0 2.0 35.0 90-91 28.118167221447866 33.0 27.5 35.0 2.0 35.0 92-93 27.76946704374256 33.0 27.0 35.0 2.0 35.0 94-95 27.361273626728682 33.0 26.5 35.0 2.0 35.0 96-97 26.69005028722137 33.0 25.5 34.5 2.0 35.0 98-99 25.748690221699583 32.0 24.0 34.0 2.0 35.0 100-101 21.290902746730485 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14036198627426444 1101 2 0.15631816554971323 1101 3 0.21318636692793547 1101 4 0.12752425215601448 1101 5 0.21610117243942284 1101 6 0.17024721301351065 1101 7 0.1833817498552648 1101 8 0.14007486531590985 1101 9 0.2604518466834307 1101 10-11 0.24010119100277905 1101 12-13 0.2803673906099178 1101 14-15 0.27837802175131543 1101 16-17 0.2827763095298721 1101 18-19 0.2740386233758869 1101 20-21 0.2561104826824945 1101 22-23 0.31127839681485625 1101 24-25 0.2718541830717598 1101 26-27 0.21392715995828837 1101 28-29 0.39890370423255206 1101 30-31 0.2539532496849972 1101 32-33 0.4440814851094146 1101 34-35 0.495980659748426 1101 36-37 0.5017431687152225 1101 38-39 0.5389003608348304 1101 40-41 0.6002382381256339 1101 42-43 0.6469996104466276 1101 44-45 0.7288453957058252 1101 46-47 0.6550949993477531 1101 48-49 0.586912320227043 1101 50-51 0.6607542252545784 1101 52-53 0.7599977362851149 1101 54-55 0.799821787471906 1101 56-57 0.7199259786491652 1101 58-59 0.703657479148923 1101 60-61 0.8066990157365765 1101 62-63 0.7792523506696298 1101 64-65 0.7254984191337357 1101 66-67 0.6924873778435696 1101 68-69 0.7594107086061541 1101 70-71 0.6396761582895962 1101 72-73 0.5838445170281297 1101 74-75 0.6838389807234897 1101 76-77 0.5987595550804059 1101 78-79 0.8816313078805749 1101 80-81 0.9122536243168362 1101 82-83 0.9301289371443389 1101 84-85 0.8896108824975677 1101 86-87 0.9763810218231512 1101 88-89 0.9825096026872906 1101 90-91 1.0225585759736617 1101 92-93 1.2468698449361355 1101 94-95 1.1534938946920512 1101 96-97 0.806579271369273 1101 98-99 0.7594748777423384 1101 100-101 1.4440404235893887 1102 1 0.08754702493313005 1102 2 0.09775555866578856 1102 3 0.1969347410879223 1102 4 0.13361513059135177 1102 5 0.1644860208147847 1102 6 0.13625436068795693 1102 7 0.1927912989556333 1102 8 0.24025747123637586 1102 9 0.19914789288137769 1102 10-11 0.2956296492247148 1102 12-13 0.30901040130432733 1102 14-15 0.43022658679696235 1102 16-17 0.39380010701041357 1102 18-19 0.25070443196032954 1102 20-21 0.2582011172418319 1102 22-23 0.316715552076559 1102 24-25 0.3522169712067367 1102 26-27 0.2661313022373548 1102 28-29 0.3887482257538579 1102 30-31 0.3412649984866931 1102 32-33 0.24924601108672562 1102 34-35 0.383054027284615 1102 36-37 0.371658278790612 1102 38-39 0.4695684455953 1102 40-41 0.4386368910870715 1102 42-43 0.3053180402464619 1102 44-45 0.31580864268899234 1102 46-47 0.4021745089462243 1102 48-49 0.24701917404595974 1102 50-51 0.35094551737587665 1102 52-53 0.33236380018664846 1102 54-55 0.3670216694479578 1102 56-57 0.3089444831172159 1102 58-59 0.3629085088476032 1102 60-61 0.2939053107482579 1102 62-63 0.3524971961546086 1102 64-65 0.44613469463534017 1102 66-67 0.3867489152860628 1102 68-69 0.4845989598044582 1102 70-71 0.5666733070479424 1102 72-73 0.6196852569659903 1102 74-75 0.4851624596598221 1102 76-77 0.38606806725614007 1102 78-79 0.3795926098773492 1102 80-81 0.6943925674003957 1102 82-83 0.8348776695912896 1102 84-85 0.8708975391683289 1102 86-87 0.8674260304942969 1102 88-89 0.9935788634720311 1102 90-91 1.0089278644578954 1102 92-93 0.9704111804131692 1102 94-95 0.8898880223987753 1102 96-97 0.7974979010872225 1102 98-99 1.0348368529573158 1102 100-101 1.4089553981781116 1103 1 0.09859767300300604 1103 2 0.08618246295807808 1103 3 0.06639620910708288 1103 4 0.06269139620607689 1103 5 0.06673254565746589 1103 6 0.14631294526577676 1103 7 0.1530177712013412 1103 8 0.097837658143483 1103 9 0.11665693298657231 1103 10-11 0.15462748899179246 1103 12-13 0.08520175802453167 1103 14-15 0.06096617306538832 1103 16-17 0.04749363570395104 1103 18-19 0.0929883499653954 1103 20-21 0.17113287031781255 1103 22-23 0.21659785460309422 1103 24-25 0.1885661188539629 1103 26-27 0.014304203054486209 1103 28-29 0.1670895425567096 1103 30-31 0.24560278143024306 1103 32-33 0.2964027805992657 1103 34-35 0.17333532718129874 1103 36-37 0.06680274436777012 1103 38-39 0.13544703815658465 1103 40-41 0.2304194941677551 1103 42-43 0.15579528735833037 1103 44-45 0.06490609971914552 1103 46-47 0.3002326473889809 1103 48-49 0.2833234146771275 1103 50-51 0.11027766492917834 1103 52-53 0.33705849117941256 1103 54-55 0.3820200257924782 1103 56-57 0.21326318791668797 1103 58-59 0.14429058830659613 1103 60-61 0.04970213144892455 1103 62-63 0.133987874279903 1103 64-65 0.08237318904691193 1103 66-67 0.16996543167565648 1103 68-69 0.2914298159141637 1103 70-71 0.08739070396725879 1103 72-73 0.19269878190258893 1103 74-75 0.23479703982324907 1103 76-77 0.009817715044249553 1103 78-79 0.1719563625776388 1103 80-81 0.15421106087273628 1103 82-83 0.2758484242639696 1103 84-85 0.2454998332417766 1103 86-87 0.13172143060700492 1103 88-89 0.3574665783212829 1103 90-91 0.32362625367551345 1103 92-93 0.26944641334263153 1103 94-95 0.17280809378038597 1103 96-97 0.08295096298258997 1103 98-99 0.004665501872498368 1103 100-101 0.29757425795081716 1104 1 -0.04809079624682511 1104 2 -0.017641083640157262 1104 3 0.027577361176263082 1104 4 -0.012848902650240746 1104 5 0.038960116345826634 1104 6 0.04216130619240488 1104 7 -0.005339294952641183 1104 8 0.04183682282483403 1104 9 -0.010806035670782421 1104 10-11 -0.024365995139554286 1104 12-13 0.006031247646753002 1104 14-15 0.09337272897570159 1104 16-17 0.09554409584765722 1104 18-19 -0.0034227015302690234 1104 20-21 0.04374833252587251 1104 22-23 0.04428703942979695 1104 24-25 -0.006978792455690552 1104 26-27 0.020286622087482442 1104 28-29 0.05788276478882892 1104 30-31 -0.0891473366351434 1104 32-33 -0.10819858464860488 1104 34-35 -0.1916711436644576 1104 36-37 -0.029390856056785708 1104 38-39 0.05192139952577435 1104 40-41 -0.05338552598239232 1104 42-43 -0.09799190563572324 1104 44-45 -0.024170528314037654 1104 46-47 -0.23499718019243687 1104 48-49 -0.22914745214798415 1104 50-51 -0.3188860920122494 1104 52-53 -0.38210536634510817 1104 54-55 -0.2565853943910099 1104 56-57 -0.2811595992312803 1104 58-59 -0.2209249516857099 1104 60-61 -0.06340786909709095 1104 62-63 -0.07201660118884234 1104 64-65 -0.054531418904140594 1104 66-67 0.06483346665558543 1104 68-69 0.037350213495187745 1104 70-71 -0.03571701669528693 1104 72-73 -0.026074358196250813 1104 74-75 0.06317905058468298 1104 76-77 -0.1434784648098173 1104 78-79 -0.0204685569319345 1104 80-81 0.05441814230576725 1104 82-83 0.041264819116793916 1104 84-85 -0.10009086732426908 1104 86-87 -0.16288334009706773 1104 88-89 -0.025019797459073345 1104 90-91 0.1839099890059508 1104 92-93 0.08078306208635055 1104 94-95 0.23938057156349402 1104 96-97 0.231857169725874 1104 98-99 0.31021071972111613 1104 100-101 0.6744612686499813 1105 1 -0.06701205479632222 1105 2 -0.05058924673605247 1105 3 0.009308776067761926 1105 4 0.05018216390874386 1105 5 0.02064321946660641 1105 6 -0.05695818855938484 1105 7 0.0046614738548669266 1105 8 0.032978116405011804 1105 9 -0.17343599839871615 1105 10-11 0.008409476602821542 1105 12-13 -4.22670704956829E-4 1105 14-15 0.06371499232177058 1105 16-17 0.03754256334254791 1105 18-19 0.010065340958071545 1105 20-21 0.043428267044838265 1105 22-23 0.011901197472930392 1105 24-25 -0.042223274403703215 1105 26-27 -0.039181219112649046 1105 28-29 0.03573588260520921 1105 30-31 -0.052036580274894106 1105 32-33 -0.11698447501015607 1105 34-35 -0.04410260772711183 1105 36-37 -0.10821685215606891 1105 38-39 -0.06025644167668531 1105 40-41 -0.15021079229521916 1105 42-43 -0.1261505837885153 1105 44-45 -0.1701142325586673 1105 46-47 -0.03600726782951824 1105 48-49 -0.10309739697268583 1105 50-51 5.358539983291166E-5 1105 52-53 -0.08507751577062805 1105 54-55 -0.06693138331785775 1105 56-57 -0.09618962337090409 1105 58-59 -0.19793900088800598 1105 60-61 -0.2713865580317716 1105 62-63 -0.28887327479800007 1105 64-65 -0.28721159103340455 1105 66-67 -0.06535804538438583 1105 68-69 -0.1686979788897176 1105 70-71 -0.1273879048351887 1105 72-73 -0.2494819221007596 1105 74-75 -0.19657197191768816 1105 76-77 -0.16060731021192254 1105 78-79 0.005948690927656486 1105 80-81 -0.08474449392820205 1105 82-83 -0.03955833448513957 1105 84-85 -0.21873226600577667 1105 86-87 -0.15645288082632902 1105 88-89 -0.20117159768804171 1105 90-91 0.1465065748038228 1105 92-93 0.2555721452055906 1105 94-95 0.1611156191285268 1105 96-97 -0.07182655633802781 1105 98-99 0.14594547323469342 1105 100-101 1.1232041057912348 1106 1 0.0047544924136389 1106 2 -0.09213506192875087 1106 3 -0.18672878793386616 1106 4 -0.07581171103664275 1106 5 0.018568626412637457 1106 6 -0.08839277332967299 1106 7 -0.06898129894574367 1106 8 -0.041164937301374493 1106 9 -0.10084177213169454 1106 10-11 -0.03575575339208115 1106 12-13 -0.13558966327743605 1106 14-15 -0.12392719685604447 1106 16-17 -0.16631903586196728 1106 18-19 -0.18853906128430964 1106 20-21 -0.15908505330735778 1106 22-23 -0.10623581061766174 1106 24-25 -0.020011513922305824 1106 26-27 -0.0817347134680162 1106 28-29 -0.11835672056136559 1106 30-31 -0.03378298538120106 1106 32-33 -0.07442684799929822 1106 34-35 -0.03388220632228922 1106 36-37 -0.17647144577484397 1106 38-39 -0.031935009243191814 1106 40-41 0.2098304010962906 1106 42-43 0.2768113363166904 1106 44-45 0.13353412162276612 1106 46-47 0.15833739977066585 1106 48-49 0.24501385319462798 1106 50-51 0.23148699925107508 1106 52-53 0.30761567381976107 1106 54-55 0.39574293151655837 1106 56-57 0.23424086387147014 1106 58-59 0.3608310335944154 1106 60-61 0.2801290589289209 1106 62-63 0.23207102320503026 1106 64-65 0.17547305543973124 1106 66-67 0.1171388729173799 1106 68-69 0.05594835189766911 1106 70-71 0.2323997042238659 1106 72-73 0.3738973594738191 1106 74-75 0.22983553592345274 1106 76-77 0.1367124560726367 1106 78-79 0.37123996393019 1106 80-81 0.30856273938981715 1106 82-83 0.4263459949995436 1106 84-85 0.2547774123289841 1106 86-87 0.34585865114217285 1106 88-89 0.5752562175629699 1106 90-91 0.70801805222127 1106 92-93 0.6769126730329411 1106 94-95 0.661673412823145 1106 96-97 0.6021754590202981 1106 98-99 0.5209721346023777 1106 100-101 0.8798697921001839 1107 1 -0.12679411983422995 1107 2 -0.0644013408657571 1107 3 -0.11742004810569284 1107 4 -0.06644058414917708 1107 5 -0.02944249376200503 1107 6 -0.017235485584841115 1107 7 -0.06642295264758502 1107 8 -0.022817015960605147 1107 9 0.04566373178618477 1107 10-11 -0.0247812754125718 1107 12-13 0.0939934310491779 1107 14-15 0.22889383903692107 1107 16-17 0.07884695287388155 1107 18-19 0.05788523766369735 1107 20-21 -0.03966594625023134 1107 22-23 0.016810352413116902 1107 24-25 -0.09823457402453784 1107 26-27 -0.06270576625766466 1107 28-29 -0.10846035204211546 1107 30-31 -0.06263631251525936 1107 32-33 -0.04415392410225394 1107 34-35 -0.1303231956198303 1107 36-37 -0.11370736340705179 1107 38-39 -0.06201626923619585 1107 40-41 -0.13990739076129444 1107 42-43 -0.20399854455364164 1107 44-45 -0.24586629591109244 1107 46-47 -0.3683830932732661 1107 48-49 -0.23169978488431298 1107 50-51 -0.18462450695668053 1107 52-53 -0.22368423387893444 1107 54-55 -0.28426374920851316 1107 56-57 -0.2151819893437832 1107 58-59 -0.1773596041282488 1107 60-61 -0.2928121245544162 1107 62-63 -0.31387810515980874 1107 64-65 -0.32636013185304336 1107 66-67 -0.1576111042709485 1107 68-69 -0.19739992021555253 1107 70-71 0.011442517263063223 1107 72-73 0.052929901528688816 1107 74-75 -0.040657485080249245 1107 76-77 -0.431736203449276 1107 78-79 -0.03352153143342207 1107 80-81 0.1621945640347704 1107 82-83 0.12923129332349248 1107 84-85 0.08365180873670752 1107 86-87 0.05328656740246984 1107 88-89 0.2031146118688092 1107 90-91 0.3234475238020522 1107 92-93 0.2681105311658847 1107 94-95 0.31524732562340674 1107 96-97 0.3259564788831888 1107 98-99 0.2546464279334337 1107 100-101 0.7008722126593945 1108 1 0.0011215740570449384 1108 2 -0.02835729256648989 1108 3 -0.03437766996174929 1108 4 -0.08824642964600571 1108 5 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13 6899.0 14 8348.0 15 10860.0 16 14522.0 17 20410.0 18 19869.0 19 13397.0 20 12282.0 21 12929.0 22 14169.0 23 15544.0 24 17734.0 25 21000.0 26 24984.0 27 30087.0 28 37021.0 29 44641.0 30 53964.0 31 65706.0 32 81398.0 33 103581.0 34 139677.0 35 200450.0 36 313205.0 37 475654.0 38 336610.0 39 21642.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.31835828728982 19.813461885780654 15.026932919747301 21.84124690718222 2 15.408280766875428 21.937729880415223 39.203228375743414 23.450760976965935 3 20.23741316672365 25.87950978881997 30.412451018330323 23.470626026126062 4 12.903453043344316 17.136305232350633 37.83981913882429 32.12042258548076 5 12.874313644825403 38.7093986749095 34.29902074046523 14.117266939799865 6 30.243339807867876 36.94504660537775 17.30358491345789 15.50802867329649 7 26.984109839163544 34.034088987906266 21.27039572305022 17.711405449879962 8 25.291541903513153 34.18079918548602 20.158986140488633 20.368672770512187 9 25.880636883807778 17.755925701898402 21.397522645216796 34.965914769077024 10-11 24.72989638240079 26.518690052635336 27.206922429565957 21.54449113539792 12-13 26.89281514515105 24.309818687986294 27.37246450590034 21.424901660962313 14-15 23.337057486745913 25.57572922727809 27.153539847737072 23.93367343823893 16-17 21.10466459651832 28.707927804045998 27.981051192025987 22.206356407409704 18-19 20.9818828873168 28.236617625511418 29.730206333522435 21.051293153649343 20-21 22.518841271212867 26.387970515195118 29.166283141288872 21.926905072303143 22-23 21.88715149283731 26.355824826896995 29.320929966074438 22.43609371419125 24-25 22.418788109899275 26.64454899294063 29.35842935556466 21.57823354159544 26-27 22.136356890870907 25.9200617272355 30.346116782068673 21.597464599824924 28-29 21.477264615604255 27.152234841511657 30.036283066149206 21.33421747673488 30-31 21.715809573544824 26.856161076901692 29.45268267392015 21.975346675633332 32-33 21.62782572105902 26.82023492416529 29.52077799610025 22.031161358675437 34-35 22.442859011330633 26.33079676036888 29.403355489895468 21.822988738405023 36-37 22.45517088498653 26.028208861958895 29.952736313150634 21.563883939903942 38-39 22.240051852834704 26.503233169845043 29.912040402000372 21.344674575319882 40-41 21.836235920888818 26.798836428941435 29.853140012219935 21.511787637949812 42-43 21.308248074589844 27.111611211206792 28.97323433918731 22.606906375016056 44-45 21.888574370438032 26.748749329316475 28.788237766556346 22.574438533689154 46-47 22.502389802008633 27.138852251650203 28.18942588631657 22.169332060024594 48-49 22.3217512283606 27.052885546730536 28.417167109874438 22.20819611503443 50-51 21.98104590357174 27.326890067649597 28.90842532128274 21.783638707495932 52-53 22.125062459847243 27.78275056260825 28.112989394036163 21.97919758350835 54-55 21.578656202215868 27.115909509300412 28.505875921869777 22.799558366613944 56-57 22.211050414958805 27.540425140229402 27.706859500095803 22.541664944715993 58-59 22.599468761065523 26.9773058784744 28.01132451879953 22.411900841660554 60-61 22.053796034932272 27.04759570013063 28.86726070593882 22.03134755899828 62-63 22.208819706128764 26.94703311029376 28.74019427360607 22.103952909971408 64-65 21.84215865191799 27.679154264696628 28.425925838958637 22.052761244426744 66-67 21.780702837829015 27.315448596958962 27.953087789215335 22.95076077599668 68-69 21.434913114872277 27.531724113801754 28.32305116927378 22.71031160205219 70-71 22.337355497030103 27.324774299228693 27.64503364378539 22.69283655995582 72-73 21.964404461793357 26.870883755179943 28.09558986516187 23.069121917864834 74-75 21.552521687186058 28.259206487558753 27.433750295862435 22.75452152939276 76-77 21.983007002164623 27.023516956673944 27.84695251962019 23.146523521541244 78-79 21.808982759203673 27.101914746535122 28.200503623610384 22.888598870650824 80-81 22.068971185105436 27.587276503468754 27.61444419528334 22.72930811614247 82-83 21.827086722445348 27.276614469645455 28.058348768273028 22.837950039636173 84-85 21.660558437996627 27.342502564141096 27.697044381243636 23.29989461661864 86-87 21.59280102791396 27.439038155473867 27.85166640956276 23.116494407049412 88-89 21.87207265338235 27.03965012872071 27.586088164015372 23.502189053881573 90-91 21.573608306492634 26.778194867836795 27.965829557258626 23.68236726841194 92-93 21.714705959702595 27.577924529719617 27.649330693426403 23.05803881715138 94-95 21.565858977875124 27.327897291590748 27.520137430448848 23.586106300085284 96-97 20.889027897825613 27.076428381398255 27.89836568226267 24.13617803851346 98-99 21.324871980794303 27.378569603750975 27.15864519425482 24.137913221199906 100-101 21.45654597074848 26.520603965652583 28.015278772795295 24.007571290803643 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1460.0 1 963.5 2 570.0 3 661.5 4 608.5 5 650.0 6 1027.5 7 1972.5 8 3138.0 9 3679.0 10 3350.5 11 2932.0 12 2991.5 13 3173.0 14 3291.0 15 3418.0 16 3539.0 17 3685.0 18 3930.5 19 4253.5 20 4718.0 21 5317.0 22 6225.5 23 7447.0 24 8444.0 25 9941.0 26 12529.0 27 15437.0 28 17567.0 29 19836.5 30 22616.5 31 26237.5 32 30997.0 33 35388.5 34 39854.5 35 44660.0 36 51597.0 37 57184.5 38 58938.5 39 60079.5 40 60285.0 41 60418.5 42 60612.0 43 62351.5 44 65665.0 45 68146.5 46 71972.5 47 87207.0 48 127251.0 49 126649.0 50 88266.5 51 73948.5 52 68221.5 53 65962.0 54 64014.0 55 59882.5 56 54687.5 57 49885.5 58 46539.5 59 42133.5 60 34809.0 61 27967.0 62 22161.0 63 16727.5 64 11559.5 65 7703.5 66 5556.5 67 4421.0 68 3514.5 69 2589.5 70 1832.0 71 1141.5 72 687.0 73 429.5 74 277.5 75 148.0 76 78.5 77 37.0 78 18.5 79 9.5 80 6.5 81 4.5 82 2.5 83 2.0 84 0.5 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4099338395242156 2 0.0 3 0.0 4 0.0 5 1.8784916463476486E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011740572789672803 16-17 0.0024420391402519433 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0024185579946725978 36-37 0.00673908878127219 38-39 0.012468488302632518 40-41 0.0028646997606801643 42-43 7.748778041184051E-4 44-45 4.931040571662578E-4 46-47 3.5221718369018415E-4 48-49 2.582926013728017E-4 50-51 6.105097850629858E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.817737469521473E-4 60-61 0.002230708830037833 62-63 0.0 64-65 9.392458231738243E-5 66-67 0.0013619064436020453 68-69 0.0015732367538161558 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.1253188739569675 78-79 0.0 80-81 2.3481145579345607E-5 82-83 0.0 84-85 0.0 86-87 0.1465223484151166 88-89 0.43552828820570233 90-91 0.3345828433600956 92-93 0.0 94-95 0.0 96-97 3.991794748488754E-4 98-99 0.0 100-101 3.991794748488754E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2129368.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.558637781430846 #Duplication Level Percentage of deduplicated Percentage of total 1 70.10550709628231 29.835948829942083 2 14.831211297884945 12.623922989731003 3 5.816962585625509 7.426860110093145 4 2.8360576565424385 4.827950021281731 5 1.6062544654073296 3.41800009890382 6 1.0237583716220153 2.6141857028141295 7 0.6840780261140613 2.0379400249337167 8 0.4873522869885269 1.6592839563117319 9 0.37121576974455855 1.4218593734982334 >10 1.997982080640696 15.55791419260296 >50 0.15296230740905 4.516489457416963 >100 0.07858056454117458 5.940292003841958 >500 0.0032088663661284486 0.907215298620071 >1k 0.003983420316573246 3.9424426465182316 >5k 6.639033860955409E-4 2.0718791327997543 >10k+ 2.2130112869851365E-4 1.1978161606903777 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15210 0.7142964485236933 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10226 0.4802363893887764 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8953 0.4204533927437625 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8635 0.40551938415529865 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7601 0.35696037509721196 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7559 0.3549879588685469 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5831 0.27383711974632846 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5418 0.254441693497789 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4991 0.23438879517302785 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4685 0.22001833407846835 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4224 0.1983687178543117 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3768 0.1769539130859485 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3705 0.17399528874295095 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3491 0.16394535843499103 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3477 0.16328788635876937 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3372 0.1583568457871068 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3281 0.15408327729166588 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3210 0.1507489546193988 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2989 0.14037028827332806 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2878 0.13515747395471334 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2586 0.1214444849363755 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2438 0.11449406584488919 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2420 0.11364874460403275 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.6962291158691214E-5 2 0.0 0.0 0.0 0.0 4.6962291158691214E-5 3 0.0 0.0 0.0 0.0 4.6962291158691214E-5 4 0.0 0.0 0.0 0.0 4.6962291158691214E-5 5 0.0 0.0 0.0 0.0 4.6962291158691214E-5 6 0.0 0.0 0.0 0.0 4.6962291158691214E-5 7 0.0 0.0 0.0 0.0 4.6962291158691214E-5 8 0.0 0.0 0.0 0.0 9.392458231738243E-5 9 0.0 0.0 0.0 0.0 9.392458231738243E-5 10-11 4.6962291158691214E-5 0.0 0.0 0.0 9.392458231738243E-5 12-13 4.6962291158691214E-5 0.0 0.0 0.0 9.392458231738243E-5 14-15 9.392458231738243E-5 0.0 0.0 0.0 1.1740572789672803E-4 16-17 1.4088687347607365E-4 0.0 0.0 7.044343673803682E-5 1.4088687347607365E-4 18-19 1.8784916463476488E-4 0.0 0.0 9.392458231738243E-5 1.4088687347607365E-4 20-21 2.348114557934561E-4 0.0 0.0 3.052548925314929E-4 1.4088687347607365E-4 22-23 2.348114557934561E-4 0.0 0.0 7.279155129597139E-4 1.4088687347607365E-4 24-25 2.817737469521473E-4 0.0 0.0 0.0015262744626574646 1.4088687347607365E-4 26-27 2.817737469521473E-4 0.0 0.0 0.0028177374695214728 1.4088687347607365E-4 28-29 3.052548925314929E-4 0.0 0.0 0.006833013363589572 1.4088687347607365E-4 30-31 3.2873603811083853E-4 0.0 0.0 0.017845670640302663 1.4088687347607365E-4 32-33 3.2873603811083853E-4 0.0 0.0 0.040598900706688555 3.2873603811083853E-4 34-35 3.2873603811083853E-4 0.0 0.0 0.07373079711914521 4.2266062042822095E-4 36-37 3.2873603811083853E-4 0.0 0.0 0.12367519376641331 4.2266062042822095E-4 38-39 3.756983292695297E-4 0.0 0.0 0.19543357465689348 4.2266062042822095E-4 40-41 3.756983292695297E-4 0.0 0.0 0.2733205345435829 4.2266062042822095E-4 42-43 4.4614176600756656E-4 0.0 0.0 0.35639682760330765 4.4614176600756656E-4 44-45 5.635474939042946E-4 0.0 0.0 0.45767100848702524 4.696229115869122E-4 46-47 6.809532218010226E-4 0.0 0.0 0.580148663828892 4.931040571662578E-4 48-49 7.044343673803683E-4 0.0 0.0 0.7118778905290208 5.165852027456034E-4 50-51 7.044343673803683E-4 0.0 0.0 0.8543145196133313 5.165852027456034E-4 52-53 7.044343673803683E-4 0.0 0.0 0.9967041864064831 5.40066348324949E-4 54-55 7.044343673803683E-4 0.0 0.0 1.154262673243892 5.870286394836402E-4 56-57 7.513966585390594E-4 0.0 0.0 1.3458218588801936 6.105097850629858E-4 58-59 7.513966585390594E-4 0.0 0.0 1.5605804163488886 6.105097850629858E-4 60-61 7.513966585390594E-4 0.0 0.0 1.8087761251225716 6.105097850629858E-4 62-63 7.513966585390594E-4 0.0 0.0 2.055281191414542 6.105097850629858E-4 64-65 7.513966585390594E-4 0.0 0.0 2.302608097801789 6.105097850629858E-4 66-67 7.513966585390594E-4 0.0 0.0 2.584781024228785 6.105097850629858E-4 68-69 7.513966585390594E-4 0.0 0.0 2.905979614608654 6.105097850629858E-4 70-71 8.218400952770962E-4 0.0 0.0 3.2608266866037248 6.574720762216771E-4 72-73 8.453212408564419E-4 0.0 0.0 3.631053908953267 7.983589496977507E-4 74-75 8.453212408564419E-4 0.0 0.0 4.021263586190832 8.453212408564419E-4 76-77 8.453212408564419E-4 0.0 0.0 4.421922373211206 8.453212408564419E-4 78-79 8.453212408564419E-4 0.0 0.0 4.896147589331671 8.453212408564419E-4 80-81 0.0010331704054912067 0.0 0.0 5.422054806872273 8.453212408564419E-4 82-83 0.001080132696649898 0.0 0.0 5.942256106037096 8.688023864357876E-4 84-85 0.001080132696649898 0.0 0.0 6.502868456743973 8.922835320151331E-4 86-87 0.001080132696649898 0.0 0.0 7.099406960187248 8.922835320151331E-4 88-89 0.001080132696649898 0.0 0.0 7.706746790597022 8.922835320151331E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10090 0.0 24.033976 1 TACATGG 10030 0.0 23.938736 2 ACATGGG 10230 0.0 22.357492 3 GAGTACT 8790 0.0 21.7855 12-13 CATGGGG 4175 0.0 21.367466 4 AGTACTT 9245 0.0 20.944311 12-13 GTACTTT 9300 0.0 20.284626 14-15 CATGGGA 5840 0.0 19.500692 4 GTAAGCG 340 0.0 18.841112 94-95 TAGACGC 180 1.243609E-4 18.453436 4 TACTTTT 10700 0.0 17.918861 14-15 ATTGCCG 135 0.009458212 17.574697 8 ACTTTTT 10775 0.0 17.506817 16-17 ATAGCGT 245 3.801444E-6 17.431232 6 GGATCGT 195 2.2942194E-4 17.03394 6 GTATTAC 490 2.7284841E-11 15.556894 1 AGTACAT 7690 0.0 15.4881315 2 ATATAGG 890 0.0 15.461786 2 CCGTAAA 215 4.8270117E-4 15.449387 2 TTTGCGT 615 0.0 15.431443 3 >>END_MODULE