FastQCFastQC Report
Thu 26 May 2016
SRR938911_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938911_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390128
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT216290.9049306145946996No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT110410.4619417872180904No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT90630.3791847131199668No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC79850.3340825261241239No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT61930.2591074620271383No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61480.25722471767202426No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT51680.21622272949398524No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC50020.2092774947617868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47660.19940354658829987No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC47330.19802286739454958No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG45520.19045005121064645No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA42830.17919542384340922No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC42260.17681061432693143No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA34210.1431304097521137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30100.1259346779754055No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC27980.11706486012464604No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG27290.11417798544680452No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT27110.1134248877047589No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA25740.10769297711252285No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC24530.10263048673543843No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG67750.022.9095331
TACATGG68650.022.286322
GAGTACT60850.021.35743112-13
ACATGGG67850.021.2226753
TTTGCGT6750.021.0520253
CTTTGCG7300.020.1147792
GTACTTT67550.019.0300114-15
AGTACTT66400.017.36193712-13
CATGGGG35100.017.2734584
AGAGTAC106050.016.60697210-11
CGTACAC2751.1591939E-515.50194553
CTTACAC12100.015.2670683
TTGCGTT9050.015.1783944
CATGGGA42300.015.11714654
GTATAGG6950.015.0250411
ACTTTTT86700.015.0197516-17
GTATAGC9050.014.6854491
AGTACAT52450.014.6300542
GTAAGCG2951.8189894E-1214.51098794-95
GAGTACA68900.014.1914181
Produced by FastQC (version 0.11.5)