##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938911_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2390128 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89793182624529 33.0 31.0 34.0 30.0 34.0 2 31.9707442446597 33.0 31.0 34.0 30.0 34.0 3 32.03484959801316 34.0 31.0 34.0 30.0 34.0 4 35.55751784004873 37.0 35.0 37.0 33.0 37.0 5 35.59946161879196 37.0 35.0 37.0 33.0 37.0 6 35.691371759169385 37.0 35.0 37.0 33.0 37.0 7 35.620037922655186 37.0 35.0 37.0 33.0 37.0 8 35.611121663777006 37.0 35.0 37.0 33.0 37.0 9 37.23783454275252 39.0 37.0 39.0 34.0 39.0 10-11 37.1290455992315 39.0 37.0 39.0 33.0 39.0 12-13 37.03874646044061 39.0 37.0 39.0 33.0 39.0 14-15 38.257769667565924 40.0 38.0 41.0 33.0 41.0 16-17 38.19038603790257 40.0 38.0 41.0 33.0 41.0 18-19 38.10344341390921 40.0 38.0 41.0 33.0 41.0 20-21 38.209100516792404 40.0 38.0 41.0 33.0 41.0 22-23 38.15533686898777 40.0 38.0 41.0 33.0 41.0 24-25 38.074239957023224 40.0 38.0 41.0 33.0 41.0 26-27 37.95672491180389 40.0 38.0 41.0 33.0 41.0 28-29 37.85058854588541 40.0 38.0 41.0 33.0 41.0 30-31 37.69543262954954 40.0 37.5 41.0 32.0 41.0 32-33 37.517903643654236 40.0 37.0 41.0 32.0 41.0 34-35 37.20611741295863 40.0 37.0 41.0 31.0 41.0 36-37 36.946547214207776 40.0 36.5 41.0 30.5 41.0 38-39 36.72306232134848 39.5 36.0 41.0 30.0 41.0 40-41 36.62479938312927 39.0 36.0 41.0 30.0 41.0 42-43 36.43631491702537 39.0 36.0 41.0 30.0 41.0 44-45 36.411145135323295 39.0 36.0 41.0 30.0 41.0 46-47 36.245387276329964 39.0 36.0 41.0 29.5 41.0 48-49 36.037966167502326 39.0 36.0 41.0 28.5 41.0 50-51 34.937672375705404 38.0 34.0 40.0 26.5 40.5 52-53 35.068514322245505 38.5 34.5 39.5 27.5 40.5 54-55 35.59389559889679 39.0 35.0 40.5 28.0 41.0 56-57 35.49995083945295 39.0 35.0 41.0 27.5 41.0 58-59 35.30318397173708 39.0 35.0 40.5 27.0 41.0 60-61 34.96840713133355 38.0 34.5 40.0 26.0 41.0 62-63 34.62773918384288 38.0 34.0 40.0 26.0 41.0 64-65 34.25183797687823 37.0 34.0 40.0 25.5 41.0 66-67 33.816015292904815 37.0 33.5 39.5 24.5 41.0 68-69 33.373556981048715 36.0 33.0 39.0 23.0 41.0 70-71 32.90590943246554 35.5 32.5 39.0 22.0 40.0 72-73 32.45574588473923 35.0 32.0 38.0 21.5 40.0 74-75 31.9894292272213 35.0 32.0 37.0 20.0 39.5 76-77 31.562673003286854 35.0 31.0 37.0 18.5 39.0 78-79 31.156014447761795 35.0 31.0 36.0 17.5 39.0 80-81 30.680663127665127 34.5 31.0 36.0 12.5 37.5 82-83 30.20391125496208 34.0 30.0 35.0 7.5 37.0 84-85 29.775601348547024 34.0 30.0 35.0 4.5 37.0 86-87 29.35957551227382 34.0 29.5 35.0 2.0 36.0 88-89 28.870424931217073 34.0 29.0 35.0 2.0 36.0 90-91 28.39595431709097 34.0 29.0 35.0 2.0 35.5 92-93 27.905051319427244 34.0 27.5 35.0 2.0 35.0 94-95 27.360004568792966 33.5 26.5 35.0 2.0 35.0 96-97 26.70036353701559 33.0 25.0 35.0 2.0 35.0 98-99 25.86071771051592 33.0 24.0 35.0 2.0 35.0 100-101 22.603103473956207 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18550112105195282 1101 2 0.3122164258088951 1101 3 0.34589276629134247 1101 4 0.2803210323234495 1101 5 0.10189686686372568 1101 6 0.21886994928640746 1101 7 0.2552326690469542 1101 8 0.2927646980073675 1101 9 0.27898991205370294 1101 10-11 0.15565509540984834 1101 12-13 0.22084838512836313 1101 14-15 0.2679455913142519 1101 16-17 0.34907223720488645 1101 18-19 0.4295982010098385 1101 20-21 0.33563732082217257 1101 22-23 0.4823602039509467 1101 24-25 0.4747515032278571 1101 26-27 0.5197137969118941 1101 28-29 0.46930514094279374 1101 30-31 0.3402747934435766 1101 32-33 0.35773959872089733 1101 34-35 0.38583444089170627 1101 36-37 0.3346458880938954 1101 38-39 0.49639918245992476 1101 40-41 0.2909538731311301 1101 42-43 0.38158876859210267 1101 44-45 0.4807063594033778 1101 46-47 0.29650696536027255 1101 48-49 0.3404584171893674 1101 50-51 0.09357258268706659 1101 52-53 0.25486220170903096 1101 54-55 0.3518263605558616 1101 56-57 0.34542656476572375 1101 58-59 0.23168287072688543 1101 60-61 0.2264630561617409 1101 62-63 0.29216962310667327 1101 64-65 0.1921219500218072 1101 66-67 0.2947170978185838 1101 68-69 0.2404570382640614 1101 70-71 0.28506781408494675 1101 72-73 0.12873089843246532 1101 74-75 -0.12650404025828976 1101 76-77 -0.3076310224687795 1101 78-79 -0.2075299226623457 1101 80-81 -0.20192354148491987 1101 82-83 -0.05324744167252149 1101 84-85 -0.09609210334193818 1101 86-87 -0.1165924244763552 1101 88-89 -0.17346669533618453 1101 90-91 -0.23504536771066853 1101 92-93 -0.3581084769536744 1101 94-95 -0.42608627390805154 1101 96-97 -0.5907830368468403 1101 98-99 -0.6874251664974089 1101 100-101 -0.18031353960848762 1102 1 0.1504180033216933 1102 2 0.2702209153488475 1102 3 0.3042552344948035 1102 4 0.1409194670884375 1102 5 0.34351090945814633 1102 6 0.2628995118372899 1102 7 0.2521816916362738 1102 8 0.3435305849977155 1102 9 0.36553372173234067 1102 10-11 0.35613896088641894 1102 12-13 0.3724563781452659 1102 14-15 0.5105985174020304 1102 16-17 0.5679311656097568 1102 18-19 0.5656912335605071 1102 20-21 0.5105353815125468 1102 22-23 0.4929639872435203 1102 24-25 0.5552027255628289 1102 26-27 0.5374206464317197 1102 28-29 0.4537265219185116 1102 30-31 0.4801775690977266 1102 32-33 0.42554986317990284 1102 34-35 0.4420178524787346 1102 36-37 0.3814625270711005 1102 38-39 0.4853785967729891 1102 40-41 0.401342043275946 1102 42-43 0.35635610764222037 1102 44-45 0.4148018226634278 1102 46-47 0.28094738346806736 1102 48-49 0.27457745023198754 1102 50-51 0.22180267533645548 1102 52-53 0.18588211052016135 1102 54-55 0.24707428948712362 1102 56-57 0.2600579202719828 1102 58-59 0.11048281826901274 1102 60-61 0.16792276704310183 1102 62-63 0.1031094470343561 1102 64-65 -0.17326135205638593 1102 66-67 -0.08225619157422415 1102 68-69 0.005919671798459092 1102 70-71 -0.048913990445804245 1102 72-73 -0.06383684404368495 1102 74-75 -0.19549420400381834 1102 76-77 -0.001689906232083871 1102 78-79 0.1462260229160357 1102 80-81 -0.029663281931252783 1102 82-83 -0.0850361282977552 1102 84-85 -0.08438346130992613 1102 86-87 -0.09574666412624566 1102 88-89 -0.20261516341111374 1102 90-91 -0.09906393908772415 1102 92-93 -0.04615384294303837 1102 94-95 -0.2847259993059872 1102 96-97 -0.40541062521690563 1102 98-99 -0.5572856192830073 1102 100-101 -0.29915600945578547 1103 1 0.24250926782211124 1103 2 0.29750573237121003 1103 3 0.2930957219010679 1103 4 0.3195087207576037 1103 5 0.12405090977859601 1103 6 0.28937098366962744 1103 7 0.1486683448439905 1103 8 0.0976850344625646 1103 9 0.2999229417541329 1103 10-11 0.35630709762295254 1103 12-13 0.3415280310325244 1103 14-15 0.5276227875744368 1103 16-17 0.5021693730057635 1103 18-19 0.52671155016629 1103 20-21 0.5305081031045518 1103 22-23 0.5268391835198756 1103 24-25 0.5378620098350879 1103 26-27 0.5743100867637096 1103 28-29 0.4424253098118669 1103 30-31 0.4433914742762184 1103 32-33 0.5110739811379901 1103 34-35 0.48595201177502645 1103 36-37 0.402191813906974 1103 38-39 0.7501171486540414 1103 40-41 0.6879891423915012 1103 42-43 0.539698854229421 1103 44-45 0.834341234154806 1103 46-47 0.8008886607865335 1103 48-49 0.8040671717598968 1103 50-51 0.7700733404329156 1103 52-53 0.9191845002849561 1103 54-55 0.9728306544489911 1103 56-57 0.9310901336283877 1103 58-59 0.8807869064251364 1103 60-61 0.9850965477263074 1103 62-63 0.8060319619353393 1103 64-65 0.7030121410218655 1103 66-67 0.7218253552436948 1103 68-69 0.6699906837833325 1103 70-71 0.6627930931278669 1103 72-73 0.5570711944745455 1103 74-75 0.5381995367832104 1103 76-77 0.5478948090470261 1103 78-79 0.4461053530128112 1103 80-81 0.4089214453647827 1103 82-83 0.6490648859302759 1103 84-85 0.5371180127655748 1103 86-87 0.6081949857167324 1103 88-89 0.5691370637246926 1103 90-91 0.7767479039873244 1103 92-93 0.5700186278959016 1103 94-95 0.4978415558116218 1103 96-97 0.3587786532872279 1103 98-99 0.26269841824182905 1103 100-101 0.31395024414929296 1104 1 0.19515477046761376 1104 2 0.14446340432888505 1104 3 0.10047557198925716 1104 4 0.1006980946553071 1104 5 0.16161153588089405 1104 6 0.22897085139449302 1104 7 0.12431869757767089 1104 8 0.18748331488583858 1104 9 0.19414692764478758 1104 10-11 0.24505544418379088 1104 12-13 0.2298437276815548 1104 14-15 0.37725076376491273 1104 16-17 0.35178357053663234 1104 18-19 0.3671877406424784 1104 20-21 0.2800286057500543 1104 22-23 0.25993276817595756 1104 24-25 0.37198623823015 1104 26-27 0.2899323310317854 1104 28-29 0.32254270399176477 1104 30-31 0.35473131113688083 1104 32-33 0.359748474083311 1104 34-35 0.4400773424628497 1104 36-37 0.36981471955071044 1104 38-39 0.4366195736981311 1104 40-41 0.3875730268712232 1104 42-43 0.43615498297720734 1104 44-45 0.39451815214422226 1104 46-47 0.5355998600810636 1104 48-49 0.5078315015658958 1104 50-51 0.3964429391983444 1104 52-53 0.546965033794649 1104 54-55 0.4980593801985478 1104 56-57 0.5369488196954038 1104 58-59 0.5988893094233774 1104 60-61 0.5536371121002048 1104 62-63 0.5735804628171692 1104 64-65 0.5515764046903016 1104 66-67 0.5298996497763184 1104 68-69 0.5141264862524721 1104 70-71 0.43778758166401843 1104 72-73 0.43129407807243325 1104 74-75 0.3865157845786271 1104 76-77 0.409333852256907 1104 78-79 0.39363897294226646 1104 80-81 0.38773537834538274 1104 82-83 0.6071061116798369 1104 84-85 0.418136531284091 1104 86-87 0.5170215950641754 1104 88-89 0.4979730425606732 1104 90-91 0.4148018061037284 1104 92-93 0.29284435540913023 1104 94-95 0.34105969955059834 1104 96-97 0.2263464442925205 1104 98-99 0.28255346674270854 1104 100-101 0.38342224591295704 1105 1 0.17340846061354753 1105 2 0.22109781046394872 1105 3 0.300040101163944 1105 4 0.22717583326620883 1105 5 0.1319518937595774 1105 6 0.2171060675191967 1105 7 0.21212377629383639 1105 8 0.3122130286431499 1105 9 0.27696286416199456 1105 10-11 0.3416380179345637 1105 12-13 0.28486856041116937 1105 14-15 0.3566247078234781 1105 16-17 0.33261294662444385 1105 18-19 0.404560245491993 1105 20-21 0.4428373925732956 1105 22-23 0.2921733107647313 1105 24-25 0.364642737730712 1105 26-27 0.3031653484807464 1105 28-29 0.3057953859960847 1105 30-31 0.3120715670426293 1105 32-33 0.2592613794547347 1105 34-35 0.3267100799497271 1105 36-37 0.32672651527109053 1105 38-39 0.5161903371278527 1105 40-41 0.561013651965375 1105 42-43 0.4320395635091856 1105 44-45 0.4600169388979438 1105 46-47 0.33325482998746025 1105 48-49 0.3646533756895707 1105 50-51 0.29159997753321676 1105 52-53 0.23507006361015215 1105 54-55 0.38192436338460567 1105 56-57 0.36797240789157826 1105 58-59 0.38922221632439147 1105 60-61 0.44147990921807434 1105 62-63 0.39395512068634986 1105 64-65 0.45567751318255745 1105 66-67 0.533807357308639 1105 68-69 0.468908148272412 1105 70-71 0.22879432114864073 1105 72-73 0.31224472084961974 1105 74-75 0.24720857485967684 1105 76-77 0.210282664156221 1105 78-79 0.2776282356546993 1105 80-81 0.3995251309914636 1105 82-83 0.5619538243571576 1105 84-85 0.4644684601718687 1105 86-87 0.5684822995581698 1105 88-89 0.6687522317367076 1105 90-91 0.6545178925675152 1105 92-93 0.5532855738004159 1105 94-95 0.5802989466194539 1105 96-97 0.5215529047420802 1105 98-99 0.6006910931370157 1105 100-101 0.7742654231118102 1106 1 0.15229047760331937 1106 2 0.19011619998168428 1106 3 0.23429987248022144 1106 4 0.17574933637321521 1106 5 0.1765431341458239 1106 6 0.15339527581892298 1106 7 0.1858921758178198 1106 8 0.23878461618714653 1106 9 0.292340359171547 1106 10-11 0.2777846019129484 1106 12-13 0.2833019478064429 1106 14-15 0.30887882039296954 1106 16-17 0.3068159839023821 1106 18-19 0.38059400404874566 1106 20-21 0.28140498212642484 1106 22-23 0.39637277961596595 1106 24-25 0.3436662515768276 1106 26-27 0.3581374409035689 1106 28-29 0.2862564177054807 1106 30-31 0.27307227114450683 1106 32-33 0.29864570464720686 1106 34-35 0.3084457108312222 1106 36-37 0.322600839003492 1106 38-39 0.36260508301697314 1106 40-41 0.42281660378147023 1106 42-43 0.358350988506551 1106 44-45 0.399040531666607 1106 46-47 0.2441774853253591 1106 48-49 0.2837741108418399 1106 50-51 0.2546165890386618 1106 52-53 0.1860743812373329 1106 54-55 0.35199665080248366 1106 56-57 0.4396312640445146 1106 58-59 0.5302432824440118 1106 60-61 0.7827531328828883 1106 62-63 0.6014416573450276 1106 64-65 0.2788453402925697 1106 66-67 0.37570378724712583 1106 68-69 0.5404325542252053 1106 70-71 0.5518361540459225 1106 72-73 0.47937787615623506 1106 74-75 0.5266440080401864 1106 76-77 0.37427498386470504 1106 78-79 0.4373195582895164 1106 80-81 0.4914879404312771 1106 82-83 0.48040202664241605 1106 84-85 0.5839273220748851 1106 86-87 0.6087144784237246 1106 88-89 0.7827578273454563 1106 90-91 0.8249440912460138 1106 92-93 0.9063864689512435 1106 94-95 0.894285544443111 1106 96-97 1.1733291412752145 1106 98-99 1.2056313064872164 1106 100-101 1.2811652269892768 1107 1 0.12462439169737038 1107 2 0.21745211472052617 1107 3 0.2532018016496238 1107 4 0.24557683674429143 1107 5 0.22889229089532392 1107 6 0.202241916123711 1107 7 0.16078547167746393 1107 8 0.16987896703990657 1107 9 0.15484405601519313 1107 10-11 0.15402584377110173 1107 12-13 0.386403539475765 1107 14-15 0.30709073253930086 1107 16-17 0.35729617566816074 1107 18-19 0.3664995447428936 1107 20-21 0.36284778941048046 1107 22-23 0.250880652458612 1107 24-25 0.36348003799975714 1107 26-27 0.32323831152643123 1107 28-29 0.3759161193151357 1107 30-31 0.4338337043390723 1107 32-33 0.43788762128342995 1107 34-35 0.3771388030359617 1107 36-37 0.3582093551987242 1107 38-39 0.5282359857056917 1107 40-41 0.26701643654902796 1107 42-43 0.44270444710505075 1107 44-45 0.34028135340785326 1107 46-47 0.47325631147062097 1107 48-49 0.48989123499174525 1107 50-51 0.522619986248273 1107 52-53 0.5545057054495004 1107 54-55 0.49132046247314776 1107 56-57 0.5553941056486025 1107 58-59 0.5759241475813042 1107 60-61 0.49927286967872675 1107 62-63 0.4853799219994315 1107 64-65 0.49874757335269493 1107 66-67 0.48391359325675865 1107 68-69 0.4866121554954006 1107 70-71 0.32226901140716535 1107 72-73 0.49839260763860693 1107 74-75 0.6517755490474784 1107 76-77 0.7042952235053015 1107 78-79 0.6444981716372453 1107 80-81 0.6481840485941639 1107 82-83 0.6783130832678843 1107 84-85 0.6522834879288908 1107 86-87 0.770882377865135 1107 88-89 0.6740427706148715 1107 90-91 0.8883016988227759 1107 92-93 0.7161076593885696 1107 94-95 0.6406472765684335 1107 96-97 0.7621623045259796 1107 98-99 0.6259647423512824 1107 100-101 0.9174242210998642 1108 1 0.1936184071300069 1108 2 0.20786509350697102 1108 3 0.19640927558341303 1108 4 0.19323043770525317 1108 5 0.22901708102970986 1108 6 0.17845982572818997 1108 7 0.28127149479962554 1108 8 0.2076367869026683 1108 9 0.3709300402457032 1108 10-11 0.29412458874983116 1108 12-13 0.3354721304114108 1108 14-15 0.38897066911835765 1108 16-17 0.4563452825000027 1108 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-1.7086551133442072 2316 80-81 -1.6454565360562583 2316 82-83 -1.300807226798824 2316 84-85 -1.4008527379248612 2316 86-87 -1.4212508622621804 2316 88-89 -1.4551285651982262 2316 90-91 -1.4720559969667306 2316 92-93 -1.5116460250039196 2316 94-95 -1.6197790348235763 2316 96-97 -1.970646795495096 2316 98-99 -1.9067416238119002 2316 100-101 -1.745360419923017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6009.0 3 1517.0 4 1529.0 5 1689.0 6 1972.0 7 2516.0 8 3101.0 9 3877.0 10 4952.0 11 6678.0 12 8832.0 13 13061.0 14 11263.0 15 11342.0 16 13797.0 17 17773.0 18 15743.0 19 12361.0 20 12671.0 21 13887.0 22 15798.0 23 17408.0 24 20204.0 25 23396.0 26 27380.0 27 32498.0 28 38517.0 29 46181.0 30 56328.0 31 68906.0 32 85616.0 33 110732.0 34 148397.0 35 211719.0 36 322894.0 37 491157.0 38 443505.0 39 64900.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.626890505164475 19.181617775449624 13.887153967038724 23.30433775234718 2 15.05395570481602 21.324207414355527 39.55319567684364 24.068641203984807 3 20.295865595741088 24.31958409291071 30.252977480493886 25.131572830854314 4 12.706757722291512 16.437966610517744 38.331103849816856 32.52417181737389 5 13.35767472037912 37.628092814487886 34.97427567479494 14.03995679033806 6 31.325392493067746 36.35965191357115 17.501356311368905 14.813599281992204 7 27.527749578758986 32.87475798264801 21.751609088340256 17.845883350252745 8 25.0508632945349 34.4277942159015 20.554673646031656 19.96666884353195 9 25.23547396002751 17.786337549446497 21.83955828563989 35.1386302048861 10-11 24.71511756051338 26.07995358238843 28.022853808176123 21.182075048922062 12-13 26.235977974590863 23.92149737952722 27.87019685518149 21.972327790700426 14-15 22.60065768694378 25.527785295415974 27.113668573251342 24.75788844438891 16-17 21.663134196336138 28.516977835231295 27.642016400765073 22.17787156766749 18-19 21.51755384129743 28.408511450669305 28.824069724699967 21.249864983333293 20-21 22.641120789863205 26.727380932195494 28.31061153650591 22.32088674143539 22-23 21.967960847549392 26.768411228023748 28.628449926576593 22.635177997850267 24-25 22.76774357137159 26.86523975065856 28.38852071246274 21.97849596550711 26-27 22.436965623398976 26.38624123605101 29.42373325645282 21.753059884097187 28-29 21.523222344770524 28.018498652108264 28.69478940814195 21.76348959497926 30-31 22.044885817532897 26.859598514343542 28.422171460967395 22.673344207156166 32-33 22.076211214619505 27.05785121544848 28.733058528214904 22.132879041717107 34-35 22.902490286173578 26.75358307197203 28.45232486519213 21.891601776662263 36-37 22.536310096189222 26.494860055341285 29.07832923312253 21.890500615346966 38-39 21.98361906443294 27.273564146053886 28.98229707207065 21.760519717442524 40-41 22.196004117006016 27.373950312662988 28.495893290700717 21.934152279630275 42-43 21.786126653672085 27.450121043846316 27.836952667305255 22.926799635176348 44-45 22.325523511974772 26.671346126800756 28.065282883455584 22.937847477768887 46-47 22.88761867765461 27.376002004534588 27.931537240073705 21.804842077737096 48-49 22.544259206892672 27.222148052447725 28.0328949374048 22.200697803254805 50-51 21.440744137232468 27.461021052689116 28.884113711179253 22.21412109889916 52-53 22.344283146398126 27.981183841229463 27.42979680650892 22.244736205863493 54-55 21.82245001480988 27.10328173944741 27.970810763767357 23.103457481975354 56-57 22.504465075730796 27.098333388678274 27.762556751333218 22.634644784257713 58-59 23.2560011195954 26.88495694979178 27.613198281437874 22.24584364917495 60-61 22.39873232002114 27.15774142411932 28.67243751763088 21.771088738228663 62-63 21.898717600812713 27.22581673099087 28.534995189610484 22.340470478585935 64-65 21.750401628843857 27.973488396442832 27.970103381713812 22.306006592999495 66-67 22.120332621150464 27.181667189186427 27.415066357906902 23.282933831756207 68-69 21.71620169722032 27.25278732466252 27.98874155737724 23.04226942073992 70-71 22.60706893321257 27.11876738954753 27.618265746247765 22.655897930992136 72-73 22.355214506567087 26.467062594684727 28.572209553787232 22.605513344960958 74-75 21.729274298174232 28.076394977932257 27.66965038552844 22.524680338365073 76-77 22.0994125554835 27.013892027508675 27.5693208704143 23.317374546593523 78-79 22.114379824009053 27.074392199029333 27.992283266904472 22.818944710057142 80-81 22.546147697433618 26.96280755060257 27.732505092257558 22.75853965970625 82-83 22.22432893802815 26.78992775903338 28.341909324943092 22.643833977995374 84-85 22.069543805467205 27.104981645727545 28.00004540483137 22.825429143973885 86-87 21.939007594306624 27.271796635365657 27.907542343808856 22.88165342651886 88-89 21.947159162217154 27.24198525548946 27.353045236077612 23.457810346215773 90-91 22.20547697225603 26.53309619928847 27.737408596410486 23.524018232045012 92-93 22.644606238857417 27.07552428349482 27.74689143133247 22.532978046315293 94-95 22.412991156233428 26.703305068101923 27.810678882831553 23.073024892833097 96-97 21.61163427296412 26.64702418010701 28.523047751931923 23.218293794996946 98-99 22.209692663689992 27.106614674722685 27.345519689898683 23.338172971688643 100-101 22.232441218464995 25.963393243045523 28.150536395696722 23.653629142792756 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1874.0 1 1094.0 2 347.5 3 388.0 4 356.0 5 384.0 6 597.5 7 1105.5 8 1719.0 9 2079.0 10 2101.0 11 2042.0 12 2153.0 13 2387.0 14 2687.5 15 2971.0 16 3227.5 17 3336.5 18 3375.5 19 3545.0 20 3917.5 21 4446.0 22 5127.5 23 6134.5 24 7623.0 25 9633.5 26 12475.5 27 15770.5 28 18661.5 29 22068.0 30 26417.0 31 30820.5 32 35111.5 33 39949.0 34 45858.0 35 51397.5 36 57043.0 37 63003.5 38 67281.0 39 69601.0 40 70858.5 41 72446.0 42 74277.0 43 77200.0 44 80777.0 45 83790.5 46 87994.0 47 103975.5 48 135507.5 49 134163.0 50 102828.0 51 87542.5 52 81779.0 53 77725.5 54 73382.0 55 67664.0 56 61006.5 57 53860.0 58 47342.5 59 41097.5 60 34525.5 61 27723.5 62 21795.0 63 17064.0 64 12689.5 65 9234.0 66 6684.5 67 4950.5 68 3595.5 69 2437.5 70 1613.0 71 1100.5 72 726.5 73 461.5 74 344.5 75 253.5 76 162.5 77 101.0 78 59.5 79 37.5 80 32.5 81 24.0 82 20.0 83 16.0 84 11.0 85 11.5 86 12.5 87 8.0 88 3.0 89 3.0 90 4.5 91 4.5 92 6.5 93 5.5 94 3.0 95 2.0 96 2.0 97 3.5 98 4.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32199112348794706 2 0.0861041751738819 3 0.09568525200323999 4 0.10166819517615792 5 0.11292282254339515 6 0.05422303742728423 7 0.05660784694376201 8 0.0574027834492546 9 0.06003862554641425 10-11 0.057716574175106936 12-13 0.05612670116412176 14-15 0.054453150626242605 16-17 0.05593842672861035 18-19 0.06418066312766513 20-21 0.06537306788590402 22-23 0.08053961963543375 24-25 0.09026713213685628 26-27 0.0839494788563625 28-29 0.08175294377539613 30-31 0.07629298514556543 32-33 0.06892936277889719 34-35 0.3167403586753513 36-37 0.49348821485711225 38-39 0.46620934108968226 40-41 0.44888809302263305 42-43 0.47882372826894626 44-45 0.4873588360121299 46-47 0.48202439367264016 48-49 0.4999941425731174 50-51 0.4960822181908249 52-53 0.49591486313703703 54-55 0.4873588360121299 56-57 0.4647868231324849 58-59 0.4475492525923298 60-61 0.4065263450325673 62-63 0.47790327547311273 64-65 0.5020442419820194 66-67 0.5115834800479305 68-69 0.5244279804261529 70-71 0.5205788141890308 72-73 0.5091986705314527 74-75 0.5113952056124191 76-77 0.4855597691839098 78-79 0.4661675023262353 80-81 0.471585622192619 82-83 0.4807901501509543 84-85 0.48225450687159854 86-87 0.48131313469404147 88-89 0.48882319273277414 90-91 0.5023789520895952 92-93 0.5081108626818313 94-95 0.5144075965806015 96-97 0.5070648935956568 98-99 0.5102446396176271 100-101 0.4878399817917702 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2390128.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.696029117698664 #Duplication Level Percentage of deduplicated Percentage of total 1 73.88496160434165 39.67329049666777 2 14.078625679792891 15.119325888786786 3 5.140871114538959 8.281330951698598 4 2.4319319681214835 5.223403590900537 5 1.3138000561875032 3.527292303593916 6 0.7981645624908662 2.5714960552934865 7 0.515633441129516 1.938122778826476 8 0.378988556541654 1.6280144453868193 9 0.26104739502732327 1.2615487672037882 >10 1.1038498426647279 10.148382540928633 >50 0.060620342896594334 2.214231784879032 >100 0.028226273920137046 2.625205866232559 >500 0.0010149788215659732 0.394540482929578 >1k 0.0016395811732986413 2.365733657604259 >5k 4.6845176379961177E-4 1.6584451136127394 >10k+ 1.5615058793320394E-4 1.3696352754550971 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21629 0.9049306145946996 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11041 0.4619417872180904 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9063 0.3791847131199668 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7985 0.3340825261241239 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6193 0.2591074620271383 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6148 0.25722471767202426 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5168 0.21622272949398524 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5002 0.2092774947617868 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4766 0.19940354658829987 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4733 0.19802286739454958 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4552 0.19045005121064645 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4283 0.17919542384340922 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4226 0.17681061432693143 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3421 0.1431304097521137 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3010 0.1259346779754055 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2798 0.11706486012464604 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2729 0.11417798544680452 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2711 0.1134248877047589 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2574 0.10769297711252285 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2453 0.10263048673543843 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 8.367752689395714E-5 0.0 0.0 0.0 0.0 7 8.367752689395714E-5 0.0 0.0 0.0 0.0 8 8.367752689395714E-5 0.0 0.0 0.0 0.0 9 8.367752689395714E-5 0.0 0.0 0.0 0.0 10-11 8.367752689395714E-5 0.0 0.0 0.0 0.0 12-13 1.2551629034093572E-4 0.0 0.0 0.0 4.183876344697857E-5 14-15 1.2551629034093572E-4 0.0 0.0 2.0919381723489285E-5 4.183876344697857E-5 16-17 1.2551629034093572E-4 0.0 0.0 8.367752689395714E-5 4.183876344697857E-5 18-19 1.2551629034093572E-4 0.0 0.0 1.6735505378791428E-4 4.183876344697857E-5 20-21 1.2551629034093572E-4 0.0 0.0 2.3011319895838216E-4 4.183876344697857E-5 22-23 1.2551629034093572E-4 0.0 0.0 3.9746825274629644E-4 4.183876344697857E-5 24-25 1.6735505378791428E-4 0.0 0.0 7.112589785986357E-4 4.183876344697857E-5 26-27 1.6735505378791428E-4 0.0 0.0 0.0014225179571972714 8.367752689395714E-5 28-29 2.3011319895838216E-4 0.0 0.0 0.005020651613637428 8.367752689395714E-5 30-31 2.5103258068187145E-4 0.0 0.0 0.015292068039870668 1.0459690861744643E-4 32-33 2.5103258068187145E-4 0.0 0.0 0.037069144414023016 1.8827443551140357E-4 34-35 3.3471010757582856E-4 0.0 0.0 0.06863649143476835 2.5103258068187145E-4 36-37 3.3471010757582856E-4 0.0 0.0 0.11765060281290374 2.5103258068187145E-4 38-39 3.3471010757582856E-4 0.0 0.0 0.18756317653280494 2.9287134412885E-4 40-41 3.3471010757582856E-4 0.0 0.0 0.2645046625117985 2.9287134412885E-4 42-43 3.9746825274629644E-4 0.0 0.0 0.34709438155613426 2.9287134412885E-4 44-45 4.602263979167643E-4 0.0 0.0 0.44861614106022774 3.3471010757582856E-4 46-47 5.229845430872322E-4 0.0 0.0 0.5713501536319394 3.7654887102280714E-4 48-49 5.439039248107215E-4 0.0 0.0 0.695192893434996 4.1838763446978573E-4 50-51 5.439039248107215E-4 0.0 0.0 0.8331143771379608 4.602263979167643E-4 52-53 5.439039248107215E-4 0.0 0.0 0.968651051324448 5.857426882577E-4 54-55 5.439039248107215E-4 0.0 0.0 1.131759470622494 6.275814517046785E-4 56-57 6.275814517046785E-4 0.0 0.0 1.337710783690246 6.903395968751464E-4 58-59 6.275814517046785E-4 0.0 0.0 1.5460050675110286 7.112589785986357E-4 60-61 6.275814517046785E-4 0.0 0.0 1.773984489533615 7.530977420456143E-4 62-63 6.275814517046785E-4 0.0 0.0 2.01526863833234 8.367752689395715E-4 64-65 6.275814517046785E-4 0.0 0.0 2.267221671810045 8.576946506630607E-4 66-67 6.275814517046785E-4 0.0 0.0 2.563021729380184 9.204527958335286E-4 68-69 6.275814517046785E-4 0.0 0.0 2.89963550069285 9.622915592805072E-4 70-71 6.694202151516571E-4 0.0 0.0 3.262503096068495 0.0010041303227274858 72-73 7.32178360322125E-4 0.0 0.0 3.6300147941867547 0.0010041303227274858 74-75 7.530977420456143E-4 0.0 0.0 4.0273575306427105 0.0010041303227274858 76-77 7.530977420456143E-4 0.0 0.0 4.446979408634181 0.001025049704450975 78-79 7.530977420456143E-4 0.0 0.0 4.95416981852018 0.0010459690861744644 80-81 7.530977420456143E-4 0.0 0.0 5.500458552847379 0.0011296466130684213 82-83 7.949365054925929E-4 0.0 0.0 6.02277367571946 0.001255162903409357 84-85 7.949365054925929E-4 0.0 0.0 6.583454944672419 0.0013388404303033142 86-87 7.949365054925929E-4 0.0 0.0 7.174469317124439 0.0013388404303033142 88-89 7.949365054925929E-4 0.0 0.0 7.815041704879404 0.0013806791937502928 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6775 0.0 22.909533 1 TACATGG 6865 0.0 22.28632 2 GAGTACT 6085 0.0 21.357431 12-13 ACATGGG 6785 0.0 21.222675 3 TTTGCGT 675 0.0 21.052025 3 CTTTGCG 730 0.0 20.114779 2 GTACTTT 6755 0.0 19.03001 14-15 AGTACTT 6640 0.0 17.361937 12-13 CATGGGG 3510 0.0 17.273458 4 AGAGTAC 10605 0.0 16.606972 10-11 CGTACAC 275 1.1591939E-5 15.5019455 3 CTTACAC 1210 0.0 15.267068 3 TTGCGTT 905 0.0 15.178394 4 CATGGGA 4230 0.0 15.1171465 4 GTATAGG 695 0.0 15.025041 1 ACTTTTT 8670 0.0 15.01975 16-17 GTATAGC 905 0.0 14.685449 1 AGTACAT 5245 0.0 14.630054 2 GTAAGCG 295 1.8189894E-12 14.510987 94-95 GAGTACA 6890 0.0 14.191418 1 >>END_MODULE