FastQCFastQC Report
Thu 26 May 2016
SRR938911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390128
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT222210.9296991625553108No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT105170.44001827517187364No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT85340.3570520072565151No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC77350.3236228352623792No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT61210.25609507105895585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59300.24810386724058295No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC53710.22471599847372192No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC53350.2232098029896307No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT53180.22249854401103206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51890.2171013435263718No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG49110.20547016728811174No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA45630.19091027760856322No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC42810.17911174631651525No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA36820.1540503270117751No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31580.13212681496555834No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT28380.11873841066252519No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC28080.11748324775911582No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA26450.11066352931725831No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC25950.10857159114490939No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG25180.10535000635949206No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT7750.023.2692153
GTAAGCG5050.023.02366694-95
CTTTGCG8350.022.1655222
GAGTACT63150.021.68132812-13
GTACATG68450.021.5010381
TTGCGTT9550.019.8772544
GTACTTT69800.019.78588714-15
TACATGG72350.019.6124782
ACATGGG70600.018.8886723
AGTACTT70500.017.93996212-13
ACGACCG1350.00945250717.5766475
CATGGGG32700.017.2702644
AGAGTAC117750.016.76645710-11
GTACCGG2304.1756157E-516.5667211
TGGACCG2901.0401945E-616.3644665
ACTTTTT89650.015.69579516-17
TATAGGG4702.6375346E-1015.1458342
CTCCGAC6500.014.96719194-95
TAGGACA12950.014.6585174
TACTTTT85650.014.62832314-15
Produced by FastQC (version 0.11.5)