##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938911_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2390128 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12748062028477 33.0 31.0 34.0 30.0 34.0 2 32.37018226638908 34.0 31.0 34.0 30.0 34.0 3 32.46650765147306 34.0 31.0 34.0 30.0 34.0 4 36.01656396644866 37.0 35.0 37.0 35.0 37.0 5 35.93910158786475 37.0 35.0 37.0 35.0 37.0 6 35.98458074211925 37.0 35.0 37.0 35.0 37.0 7 35.918673393224125 37.0 35.0 37.0 35.0 37.0 8 35.89061673684422 37.0 35.0 37.0 35.0 37.0 9 37.56066285989704 39.0 37.0 39.0 35.0 39.0 10-11 37.4632078282 39.0 37.0 39.0 34.0 39.0 12-13 37.37787223111063 39.0 37.0 39.0 34.0 39.0 14-15 38.668099783777265 40.0 38.0 41.0 34.0 41.0 16-17 38.77838571825442 40.0 38.0 41.0 34.5 41.0 18-19 38.81261756692528 40.0 38.0 41.0 34.0 41.0 20-21 38.7802789223004 40.0 38.0 41.0 34.0 41.0 22-23 38.706181217072896 40.0 38.0 41.0 34.0 41.0 24-25 38.62145249124733 40.0 38.0 41.0 34.0 41.0 26-27 38.454430474016455 40.0 38.0 41.0 34.0 41.0 28-29 38.3072419134038 40.0 38.0 41.0 34.0 41.0 30-31 38.13911953669427 40.0 38.0 41.0 33.0 41.0 32-33 38.06120906495384 40.0 37.5 41.0 33.0 41.0 34-35 37.96122739033223 40.0 37.0 41.0 33.0 41.0 36-37 37.82905266998253 40.0 37.0 41.0 33.0 41.0 38-39 37.69310158284409 40.0 37.0 41.0 33.0 41.0 40-41 37.5641494932489 40.0 37.0 41.0 32.0 41.0 42-43 37.3890919231104 40.0 37.0 41.0 32.0 41.0 44-45 37.160211084929344 40.0 36.5 41.0 31.0 41.0 46-47 36.89321638841099 40.0 36.0 41.0 31.0 41.0 48-49 36.58696689047616 39.0 36.0 41.0 30.0 41.0 50-51 36.33277150847151 39.0 35.5 40.5 30.0 41.0 52-53 36.097431183601884 39.0 35.0 40.0 29.5 41.0 54-55 35.80927402214442 38.5 35.0 40.0 28.5 41.0 56-57 35.65559731529023 38.0 35.0 40.0 28.0 41.0 58-59 35.37478787746932 38.0 34.5 40.0 28.0 41.0 60-61 34.97293973377158 38.0 34.0 40.0 27.0 41.0 62-63 34.56460700012719 37.0 34.0 40.0 26.0 41.0 64-65 34.15194081655878 37.0 33.0 39.5 26.0 41.0 66-67 33.669037390466116 36.0 33.0 39.0 25.5 40.0 68-69 33.15226067390533 36.0 32.0 39.0 24.5 40.0 70-71 32.58640499588307 35.0 31.5 38.0 22.5 40.0 72-73 32.03886172623391 35.0 31.0 37.0 21.5 39.0 74-75 31.497882749375766 34.5 31.0 37.0 20.0 39.0 76-77 29.5823246704779 32.5 28.5 35.0 18.0 37.0 78-79 30.564813474424803 34.0 30.0 36.0 18.0 37.5 80-81 30.52289019667566 34.0 30.0 35.0 18.0 37.0 82-83 30.074537221437513 34.0 30.0 35.0 15.0 37.0 84-85 29.585354215339095 34.0 29.0 35.0 8.5 36.0 86-87 28.874231003527846 33.5 29.0 35.0 4.0 36.0 88-89 28.137636980111527 33.0 27.0 35.0 2.0 35.0 90-91 27.50508320056499 32.5 26.0 34.5 2.0 35.0 92-93 27.05416153444502 32.0 25.5 34.0 2.0 35.0 94-95 26.432533529585026 32.0 25.0 34.0 2.0 35.0 96-97 25.47878879290147 32.0 22.5 34.0 2.0 35.0 98-99 24.151623469538034 31.0 10.0 34.0 2.0 35.0 100-101 18.442340744930817 24.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24976407152724533 1101 2 0.2015191127210656 1101 3 0.18225205307683723 1101 4 0.10554538865508789 1101 5 0.16220352829837736 1101 6 0.1752775697967337 1101 7 0.14250398853825175 1101 8 0.12866061375296312 1101 9 0.22298777028224492 1101 10-11 0.20850305063856212 1101 12-13 0.1897083479247641 1101 14-15 0.3085636877192286 1101 16-17 0.2532028129073609 1101 18-19 0.3291775816303897 1101 20-21 0.368796528123454 1101 22-23 0.23829638253922525 1101 24-25 0.2793366527015664 1101 26-27 0.28200077270791724 1101 28-29 0.25956390194811263 1101 30-31 0.3152212144545885 1101 32-33 0.3642080967763164 1101 34-35 0.42768662512108335 1101 36-37 0.5052337041841426 1101 38-39 0.38974282766031365 1101 40-41 0.36973880593122743 1101 42-43 0.3014375349190459 1101 44-45 0.42474395006214394 1101 46-47 0.49144323819730573 1101 48-49 0.5418992551778743 1101 50-51 0.4983862210335843 1101 52-53 0.4445590984131229 1101 54-55 0.48350624952497157 1101 56-57 0.3576234728416452 1101 58-59 0.6032824587881862 1101 60-61 0.4456350069774402 1101 62-63 0.49263318333262873 1101 64-65 0.6409075616028019 1101 66-67 0.2663823487180963 1101 68-69 0.4621137438188896 1101 70-71 0.4720725902642684 1101 72-73 0.5063322340684095 1101 74-75 0.39168731388307165 1101 76-77 0.2393430576335831 1101 78-79 0.5434523410443184 1101 80-81 0.6892986722092083 1101 82-83 0.5858387382055312 1101 84-85 0.5833770771636146 1101 86-87 0.9687985212502355 1101 88-89 1.1068569178735927 1101 90-91 1.0588939427948567 1101 92-93 0.943473594902354 1101 94-95 0.773817255917006 1101 96-97 0.9894997186589656 1101 98-99 1.4047219681598335 1101 100-101 1.4688653366036597 1102 1 0.1071884422392344 1102 2 0.1520896525476516 1102 3 0.13140780159572074 1102 4 0.12067477165257401 1102 5 0.21682982335217105 1102 6 0.19171497500138912 1102 7 0.17248938534561375 1102 8 0.1672050648520127 1102 9 0.20386824469160558 1102 10-11 0.19688244275171485 1102 12-13 0.2710511929747952 1102 14-15 0.3321192679861156 1102 16-17 0.3134911942334071 1102 18-19 0.351232149504618 1102 20-21 0.2822751802461596 1102 22-23 0.2764455865151447 1102 24-25 0.27019953438347244 1102 26-27 0.34075006737713665 1102 28-29 0.2926695753559372 1102 30-31 0.24501740835775365 1102 32-33 0.28505442118959223 1102 34-35 0.34318119873262987 1102 36-37 0.28125922643109647 1102 38-39 0.20995625304875176 1102 40-41 0.2614031206559133 1102 42-43 0.26914018418275276 1102 44-45 0.19155187426333242 1102 46-47 0.1700614969624965 1102 48-49 0.2485675697148082 1102 50-51 0.10526894001147014 1102 52-53 0.0023441472337992764 1102 54-55 0.11689872891097508 1102 56-57 0.04823613207113908 1102 58-59 0.05414238316745923 1102 60-61 0.19511692502591416 1102 62-63 0.2090063054807061 1102 64-65 0.21216294261437696 1102 66-67 -0.005911694190594119 1102 68-69 0.0449718035664759 1102 70-71 0.2541815338283371 1102 72-73 0.24761932147382737 1102 74-75 0.22216880425078855 1102 76-77 -0.062472037220686616 1102 78-79 0.07224283045574609 1102 80-81 0.08498949145657164 1102 82-83 0.0757957948674921 1102 84-85 0.3833249214652632 1102 86-87 0.5063599915453594 1102 88-89 0.2387182679225326 1102 90-91 0.399747446926515 1102 92-93 0.43467169126973104 1102 94-95 0.44116301435332517 1102 96-97 0.7331036955460704 1102 98-99 1.0925878411896157 1102 100-101 1.346879826177858 1103 1 0.06663594432216513 1103 2 0.07890360659494888 1103 3 0.1382087773696128 1103 4 0.128849882208236 1103 5 0.09901293717643256 1103 6 0.10860495609476573 1103 7 0.1679251589148052 1103 8 0.13823707792641926 1103 9 0.11249078522867961 1103 10-11 0.1088980607418435 1103 12-13 0.11394308475843218 1103 14-15 0.24563289517320186 1103 16-17 0.16268415792627877 1103 18-19 0.2420291917400803 1103 20-21 0.21731681887334275 1103 22-23 0.13594135655937123 1103 24-25 0.2000361443302836 1103 26-27 0.14871468155389778 1103 28-29 0.1328458435415456 1103 30-31 0.21504360524296118 1103 32-33 0.19743665537531996 1103 34-35 0.20286212948312254 1103 36-37 0.27429431984261754 1103 38-39 0.26247149450873763 1103 40-41 0.36332292373865016 1103 42-43 0.36987942503387217 1103 44-45 0.34266654371792526 1103 46-47 0.32220691771491516 1103 48-49 0.16659294944948044 1103 50-51 0.22609616474389327 1103 52-53 0.3515763661981026 1103 54-55 0.4911655966607711 1103 56-57 0.5059232555409849 1103 58-59 0.399168179488683 1103 60-61 0.2985898148547932 1103 62-63 0.41324567451231076 1103 64-65 0.5179761275788053 1103 66-67 0.5136447412705323 1103 68-69 0.45697490393178697 1103 70-71 0.36195447813391723 1103 72-73 0.44735364740473216 1103 74-75 0.4436654715583579 1103 76-77 0.4124019854031644 1103 78-79 0.6967474329360392 1103 80-81 0.6815827948277189 1103 82-83 0.5909694593871357 1103 84-85 0.6216781227726997 1103 86-87 0.6960125223086742 1103 88-89 0.7795011902772089 1103 90-91 0.7340975118178825 1103 92-93 0.8143323534821256 1103 94-95 1.231781104624428 1103 96-97 0.934123030770067 1103 98-99 1.0325767455669812 1103 100-101 1.3340943241910637 1104 1 0.05425168506290845 1104 2 0.09959915333216429 1104 3 0.07321208424791337 1104 4 0.10128154005656143 1104 5 0.03968202888719219 1104 6 0.14600023828171516 1104 7 0.1516167373980437 1104 8 0.10320621372888894 1104 9 0.18191318381774124 1104 10-11 0.18855249195877377 1104 12-13 0.17170598158382688 1104 14-15 0.20411503803033781 1104 16-17 0.1745420723883626 1104 18-19 0.16781181960604385 1104 20-21 0.24873491852061136 1104 22-23 0.17026675338476593 1104 24-25 0.16017172634087018 1104 26-27 0.023301321765536898 1104 28-29 0.16553709133695804 1104 30-31 0.019948808064832235 1104 32-33 0.13222501516368368 1104 34-35 0.3142901497653128 1104 36-37 0.19321793095372897 1104 38-39 0.22241141955282728 1104 40-41 0.19487329851819624 1104 42-43 0.11975266136543894 1104 44-45 0.17591444936166312 1104 46-47 0.32670427221226817 1104 48-49 0.12924727043713347 1104 50-51 0.21948516386206052 1104 52-53 0.25274204080127305 1104 54-55 0.4307296398706413 1104 56-57 0.2775750412552753 1104 58-59 0.40963996079379683 1104 60-61 0.2855607143256833 1104 62-63 0.2835554187803879 1104 64-65 0.20670518401619375 1104 66-67 0.09383378448041668 1104 68-69 0.022147963896514966 1104 70-71 0.1461586227547329 1104 72-73 0.24508568003260223 1104 74-75 0.24020427226382424 1104 76-77 0.22797187076471914 1104 78-79 0.26537342499952743 1104 80-81 0.3410377110534668 1104 82-83 0.2208002574471024 1104 84-85 0.46519664129121807 1104 86-87 0.3762511686931518 1104 88-89 0.785246891335408 1104 90-91 0.5909179483258207 1104 92-93 0.6037696330235711 1104 94-95 0.8452593894568778 1104 96-97 0.6177457159552517 1104 98-99 1.0206554492706807 1104 100-101 1.0823719917395636 1105 1 0.03757389407499545 1105 2 0.06310848269782099 1105 3 0.10766097842085998 1105 4 0.09470660268880948 1105 5 0.029630741131128957 1105 6 0.041195806166726356 1105 7 -0.03031748078233676 1105 8 0.048781630832998246 1105 9 0.14577014822223333 1105 10-11 0.1512251796643156 1105 12-13 0.1133919419951539 1105 14-15 0.05282600730635778 1105 16-17 -0.02287320315875263 1105 18-19 0.09307144504087717 1105 20-21 0.1732335129162692 1105 22-23 0.13009144103468628 1105 24-25 0.12153296086241738 1105 26-27 -0.01972459790295744 1105 28-29 0.11334896052421328 1105 30-31 0.1673631565851892 1105 32-33 0.17317033912477342 1105 34-35 0.23905207770289394 1105 36-37 0.2585429001880257 1105 38-39 0.37535837971616814 1105 40-41 0.3447949647561117 1105 42-43 0.37732791855743386 1105 44-45 0.4880449422849722 1105 46-47 0.508930214129407 1105 48-49 0.49397233747275493 1105 50-51 0.5444940864947228 1105 52-53 0.4664515500101203 1105 54-55 0.5616014853723641 1105 56-57 0.588905683893401 1105 58-59 0.6037509652968254 1105 60-61 0.4853483093169615 1105 62-63 0.6655544708955077 1105 64-65 0.5030148063223123 1105 66-67 0.6139882155524958 1105 68-69 0.5704524319337949 1105 70-71 0.6842860750302293 1105 72-73 0.6695865919951842 1105 74-75 0.570842275262752 1105 76-77 0.29589859856880096 1105 78-79 0.6513682521901494 1105 80-81 0.6671153217346735 1105 82-83 0.567310407231453 1105 84-85 0.7677156047825875 1105 86-87 0.7170624214076042 1105 88-89 0.8704270358125328 1105 90-91 0.688043701824764 1105 92-93 0.9365334242849528 1105 94-95 0.8407832986360653 1105 96-97 0.9795616571075918 1105 98-99 1.409577363488264 1105 100-101 1.5311256621861808 1106 1 -0.037089656278432415 1106 2 0.009211146277493754 1106 3 -0.027209766173932337 1106 4 -0.053349281240926416 1106 5 -0.01298969045119236 1106 6 0.021792697407505557 1106 7 0.09726476637941062 1106 8 0.04569632288566794 1106 9 0.03589812113601454 1106 10-11 0.10407515748143936 1106 12-13 0.08601314589099474 1106 14-15 0.04206012597542497 1106 16-17 -0.12671752053789476 1106 18-19 -0.0351292166683308 1106 20-21 -0.00701487889585195 1106 22-23 -0.04171308359507009 1106 24-25 0.07918002034916327 1106 26-27 0.0690077424719604 1106 28-29 0.023919283052485696 1106 30-31 0.029428525877882805 1106 32-33 0.061958319896987746 1106 34-35 -0.01004609123759792 1106 36-37 0.009123121858912953 1106 38-39 0.03744669292143499 1106 40-41 0.10999079696902925 1106 42-43 -0.04327345999401899 1106 44-45 0.09899228910617097 1106 46-47 0.03189160239960387 1106 48-49 0.03160796446449865 1106 50-51 0.02758493029515563 1106 52-53 -0.021442858383622365 1106 54-55 0.06255193835961137 1106 56-57 -0.0752241865439558 1106 58-59 -0.005566912746409969 1106 60-61 -0.12216844340346711 1106 62-63 -0.04495761806598608 1106 64-65 -0.0506116566339756 1106 66-67 -0.025577293263999934 1106 68-69 -0.10055807547619366 1106 70-71 -0.0027521928227542958 1106 72-73 0.020748247361837002 1106 74-75 0.17459103998392678 1106 76-77 -0.14087316474698497 1106 78-79 0.016231425857530013 1106 80-81 0.0583383450207684 1106 82-83 0.09718259216276692 1106 84-85 0.22488490097947889 1106 86-87 0.3343819184905712 1106 88-89 0.31933455042306846 1106 90-91 0.7228355802434514 1106 92-93 0.648659761247032 1106 94-95 0.8206693232001463 1106 96-97 0.8445618177713534 1106 98-99 1.0487192273344625 1106 100-101 0.955310406344644 1107 1 0.1421501653254964 1107 2 0.10030637067728776 1107 3 0.05084628815477288 1107 4 -0.0021274073270802774 1107 5 0.047523429596004974 1107 6 0.07285022603190328 1107 7 0.043109662181166186 1107 8 0.028211501690748264 1107 9 -0.07450693448043211 1107 10-11 0.010786811820359787 1107 12-13 0.06524789648035778 1107 14-15 0.060610785595976324 1107 16-17 0.10896963339774857 1107 18-19 -0.016588921165130444 1107 20-21 0.03091825329957487 1107 22-23 0.05929430861163354 1107 24-25 0.09084141056965223 1107 26-27 0.13190331399565736 1107 28-29 0.10687229029367273 1107 30-31 0.25944687776854636 1107 32-33 0.15886892457290713 1107 34-35 0.09949504116808328 1107 36-37 0.20556608275399668 1107 38-39 0.15328273110431923 1107 40-41 0.1626201550341051 1107 42-43 0.17963806856894138 1107 44-45 0.10443604174578525 1107 46-47 0.06321825753988719 1107 48-49 0.09008610857680566 1107 50-51 0.02469852361237912 1107 52-53 -0.04140692450818051 1107 54-55 -0.171541825069788 1107 56-57 -0.022945727880681943 1107 58-59 0.05064574635667185 1107 60-61 0.1595398333436293 1107 62-63 0.21058135490180518 1107 64-65 0.09062072575457591 1107 66-67 0.08177247607886784 1107 68-69 0.04469484415118785 1107 70-71 0.17347379897700677 1107 72-73 0.15720184628307976 1107 74-75 0.10437821653555446 1107 76-77 0.17832665876690257 1107 78-79 0.3304096494404156 1107 80-81 0.20181594686765436 1107 82-83 0.16864706785502648 1107 84-85 0.42119433132687334 1107 86-87 0.41043593997168415 1107 88-89 0.5059346535460385 1107 90-91 0.6487092845899767 1107 92-93 0.7271514786516029 1107 94-95 0.9029687432907672 1107 96-97 0.9828964114553891 1107 98-99 0.7909441035110198 1107 100-101 1.0569975736055817 1108 1 0.05330686268951723 1108 2 0.0010281629392281388 1108 3 0.0895369716908192 1108 4 0.05863581832181097 1108 5 -0.012691255370150145 1108 6 0.11898927286495109 1108 7 0.11358010567611387 1108 8 0.10774683835232679 1108 9 0.14852672134225742 1108 10-11 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62-63 -0.6140916026657024 2316 64-65 -0.647694378321269 2316 66-67 -0.8329207401505059 2316 68-69 -0.6261463300626886 2316 70-71 -0.512061652923073 2316 72-73 -0.6082070951260192 2316 74-75 -0.8781679454639892 2316 76-77 -0.8446522136929921 2316 78-79 -0.8578600394465887 2316 80-81 -0.8968509391667574 2316 82-83 -0.8035066735528602 2316 84-85 -1.1419736472498947 2316 86-87 -1.342492922707649 2316 88-89 -1.4281324244604257 2316 90-91 -1.4723537341022777 2316 92-93 -1.4245081091088885 2316 94-95 -1.4399852304245293 2316 96-97 -1.205432188285922 2316 98-99 -1.3157748424320168 2316 100-101 -0.6762531682769257 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 26.0 5 55.0 6 95.0 7 256.0 8 640.0 9 1219.0 10 2093.0 11 3268.0 12 4516.0 13 6291.0 14 7839.0 15 10290.0 16 13091.0 17 17934.0 18 17681.0 19 14097.0 20 14132.0 21 15386.0 22 16951.0 23 18975.0 24 21783.0 25 26176.0 26 31373.0 27 37716.0 28 45589.0 29 55510.0 30 67107.0 31 80853.0 32 100354.0 33 126919.0 34 169816.0 35 240350.0 36 372006.0 37 526426.0 38 310720.0 39 12586.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.40599053100943 19.923186258203042 14.498425755116717 23.172397455670808 2 15.347922789072385 21.89761385164309 38.79311066185577 23.961352697428755 3 20.190048399081554 24.5589775945054 30.42523245616971 24.825741550243336 4 13.078211710837243 17.044610163137705 37.72555277374266 32.15162535228239 5 13.162391302892566 38.01708527744121 34.831565506115155 13.988957913551072 6 31.24782438430076 36.828487846676 17.14397722632428 14.779710542698968 7 27.35322125007531 32.97517957197271 21.608424318697576 18.0631748592544 8 25.04840744930815 34.863279288807966 20.200131541072277 19.888181720811605 9 24.992636377633332 17.74356854528293 21.6357868699919 35.62800820709184 10-11 24.68620927414766 26.362291057215348 27.817945315062627 21.133554353574368 12-13 26.158410762938217 24.033880193864093 27.77930721701934 22.02840182617835 14-15 22.421621006471902 25.704515059297687 27.018475993592737 24.85538794063767 16-17 21.59330894758808 28.70211281527746 27.507894684542105 22.196683552592354 18-19 21.371772128596053 28.56996719829967 28.785897779191004 21.272362893913275 20-21 22.56031894526151 26.813794072953417 28.33532346384796 22.290563517937116 22-23 21.927654083798025 26.888727298286952 28.58271607210995 22.60090254580508 24-25 22.678281396043555 26.9809206711278 28.446868491451916 21.89392944137673 26-27 22.37566824363972 26.480072715786086 29.431630739364323 21.71262830120987 28-29 21.4540183621965 28.18290484861062 28.662251561422654 21.700825227770228 30-31 22.05720781481159 26.933934082191413 28.401386871330743 22.607471231666253 32-33 22.03597464236225 27.137563343887862 28.73975787070818 22.08670414304171 34-35 22.863199492179966 26.855168771913235 28.473112862333323 21.808518873573483 36-37 22.478338107563513 26.55715580032349 29.146309056250825 21.818197035862173 38-39 21.95323974308011 27.338138376885578 29.008243195179613 21.700378684854698 40-41 22.094745562724373 27.38416221056243 28.559653218855384 21.961439007857805 42-43 21.720764026223467 27.516253684890334 27.93573128663577 22.82725100225042 44-45 22.258385601480118 26.74422058852448 28.099092122059567 22.898301687935838 46-47 22.87231260682107 27.47282011802878 27.904811315031413 21.75005596011874 48-49 22.527028262444485 27.343131058856855 27.972303444559294 22.157537234139365 50-51 21.471777757462576 27.535328402317973 28.780062168699803 22.212831671519652 52-53 22.309621074687215 28.088307404457 27.285379695146034 22.316691825709754 54-55 21.82109075329857 27.165595315397333 27.89363163813821 23.119682293165887 56-57 22.450952417611106 27.193041544218556 27.71276266375692 22.643243374413423 58-59 23.218645205963757 26.96741356551281 27.51190847264032 22.30203275588311 60-61 22.32059365103672 27.317662800286683 28.540837984829864 21.820905563846733 62-63 21.844248097147563 27.315367248846904 28.354739725935694 22.485644928069835 64-65 21.652271580025936 28.08101469828056 27.824310978220872 22.44240274347263 66-67 22.105200177569284 27.27257512699509 27.269165160772584 23.35305953466304 68-69 21.662013016830752 27.34532341140461 27.846252363173384 23.14641120859125 70-71 22.51248573820554 27.15690421471328 27.522700676574928 22.807909370506255 72-73 22.284873446108325 26.514207607291322 28.32860834231472 22.87231060428563 74-75 21.618361025016235 28.066990554480764 27.490431474799674 22.824216945703327 76-77 21.915047803225047 26.984298146561102 27.48049438717001 23.62015966304385 78-79 21.924055018187936 27.07286805358558 27.83622156226282 23.166855365963666 80-81 22.40518367061751 26.95286138233169 27.562704984392006 23.079249962658793 82-83 22.012005635682616 26.780349583861113 28.13126078002115 23.076384000435123 84-85 21.786649384424727 27.048849389278036 27.849477976438024 23.315023249859212 86-87 21.639502981936683 27.163461387463745 27.738428781201037 23.458606849398535 88-89 21.60673596629916 27.067974220116504 27.250471423093693 24.07481839049065 90-91 21.836638694991137 26.347365370561704 27.595191895774008 24.22080403867315 92-93 22.230629489299318 26.948682246306472 27.57258188682782 23.24810637756639 94-95 21.90759542077354 26.499854817668567 27.697609052013767 23.894940709544127 96-97 21.044588625502193 26.522559988954562 28.369825459102078 24.063025926441163 98-99 21.53374212594472 26.88879005643212 27.21521608884545 24.362251728777707 100-101 21.51344023881671 25.499520421067167 28.36251774232857 24.624521597787556 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 580.0 1 396.5 2 230.0 3 274.0 4 265.5 5 274.0 6 451.5 7 874.0 8 1368.5 9 1646.0 10 1583.0 11 1534.0 12 1713.5 13 1822.5 14 1933.0 15 2093.5 16 2249.0 17 2421.0 18 2672.0 19 3031.5 20 3495.0 21 4116.5 22 5018.5 23 6216.5 24 7687.0 25 9714.5 26 12588.0 27 15985.5 28 19049.5 29 22325.0 30 26272.0 31 30827.5 32 35348.5 33 39898.0 34 45762.5 35 51289.0 36 57199.0 37 63257.0 38 67394.5 39 69601.5 40 70294.5 41 71795.0 42 73861.5 43 76726.0 44 80595.0 45 83605.5 46 86957.0 47 104113.5 48 135783.5 49 133008.0 50 103159.0 51 90134.0 52 85122.0 53 80378.0 54 74905.0 55 68589.5 56 62055.0 57 55031.0 58 48363.5 59 41625.5 60 34843.5 61 28028.5 62 21852.5 63 16987.5 64 12417.0 65 8834.0 66 6379.5 67 4787.5 68 3393.5 69 2193.0 70 1473.5 71 979.5 72 622.0 73 389.5 74 262.5 75 181.0 76 110.0 77 51.0 78 27.5 79 19.5 80 9.0 81 4.0 82 4.5 83 3.0 84 0.5 85 0.0 86 0.5 87 0.5 88 0.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3892678551106886 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001108727231344932 16-17 0.002949632823011989 18-19 3.3471010757582856E-4 20-21 0.0 22-23 0.0 24-25 8.367752689395714E-5 26-27 2.0919381723489285E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012760822851328462 36-37 0.023555223820648934 38-39 0.011631176238260044 40-41 0.006192136990152829 42-43 0.005501797393277682 44-45 0.00205009940890195 46-47 0.0013806791937502928 48-49 0.0034935367478227107 50-51 7.740171237691036E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 9.622915592805071E-4 60-61 0.0015480342475382072 62-63 0.0013388404303033142 64-65 7.740171237691036E-4 66-67 0.003137907258523393 68-69 0.0028659552961180323 70-71 4.183876344697857E-5 72-73 0.0 74-75 0.0 76-77 0.09043448719064419 78-79 0.003430778602652243 80-81 2.9287134412885003E-4 82-83 2.0919381723489285E-5 84-85 2.5103258068187145E-4 86-87 0.09064368100787908 88-89 0.4336797025096564 90-91 0.38274100801295996 92-93 0.0 94-95 0.0012551629034093573 96-97 2.0919381723489285E-5 98-99 0.0 100-101 4.39307016193275E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2390128.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.71645063731187 #Duplication Level Percentage of deduplicated Percentage of total 1 72.87029203639929 38.41463153063344 2 14.650967061617246 15.446939637852555 3 5.356966226930024 8.472007370031104 4 2.4764332200797536 5.221950784117346 5 1.3814512483299104 3.6412603270218296 6 0.8302325482281973 2.6260147887696847 7 0.5346772106244196 1.973039934654448 8 0.3710826111706583 1.5649726523314245 9 0.2746101397755259 1.3028824690183645 >10 1.1557708400401732 10.376937520605896 >50 0.06413568228883054 2.305385310599581 >100 0.02980920955354787 2.702986616701194 >500 0.0012700319866172738 0.4818839864718577 >1k 0.00150816298410694 2.026498747901499 >5k 6.350159933081852E-4 2.0726953370873846 >10k+ 1.587539983270463E-4 1.369912986202467 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22221 0.9296991625553108 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10517 0.44001827517187364 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8534 0.3570520072565151 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7735 0.3236228352623792 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6121 0.25609507105895585 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5930 0.24810386724058295 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5371 0.22471599847372192 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5335 0.2232098029896307 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5318 0.22249854401103206 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5189 0.2171013435263718 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4911 0.20547016728811174 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4563 0.19091027760856322 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4281 0.17911174631651525 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3682 0.1540503270117751 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3158 0.13212681496555834 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2838 0.11873841066252519 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2808 0.11748324775911582 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2645 0.11066352931725831 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2595 0.10857159114490939 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2518 0.10535000635949206 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.183876344697857E-5 7 0.0 0.0 0.0 0.0 4.183876344697857E-5 8 0.0 0.0 0.0 0.0 4.183876344697857E-5 9 0.0 0.0 0.0 0.0 8.367752689395714E-5 10-11 0.0 0.0 0.0 0.0 8.367752689395714E-5 12-13 0.0 0.0 0.0 0.0 2.3011319895838216E-4 14-15 0.0 0.0 0.0 0.0 2.719519624053607E-4 16-17 0.0 0.0 0.0 4.183876344697857E-5 3.3471010757582856E-4 18-19 4.183876344697857E-5 0.0 0.0 1.46435672064425E-4 3.5562948929931785E-4 20-21 8.367752689395714E-5 0.0 0.0 2.510325806818714E-4 4.39307016193275E-4 22-23 8.367752689395714E-5 0.0 0.0 4.39307016193275E-4 4.811457796402536E-4 24-25 1.2551629034093572E-4 0.0 0.0 7.740171237691036E-4 5.439039248107215E-4 26-27 1.2551629034093572E-4 0.0 0.0 0.0014225179571972714 6.275814517046785E-4 28-29 1.8827443551140357E-4 0.0 0.0 0.005083409758807896 6.275814517046785E-4 30-31 2.5103258068187145E-4 0.0 0.0 0.015480342475382072 6.694202151516571E-4 32-33 2.5103258068187145E-4 0.0 0.0 0.03740385452159884 7.949365054925929E-4 34-35 2.5103258068187145E-4 0.0 0.0 0.06926407288647302 8.7861403238655E-4 36-37 2.9287134412885E-4 0.0 0.0 0.1183200230280554 8.7861403238655E-4 38-39 3.3471010757582856E-4 0.0 0.0 0.18867190376414988 8.7861403238655E-4 40-41 3.3471010757582856E-4 0.0 0.0 0.26624097119484813 8.7861403238655E-4 42-43 3.3471010757582856E-4 0.0 0.0 0.3492072391102066 8.995334141100393E-4 44-45 3.3471010757582856E-4 0.0 0.0 0.4515030157380693 9.622915592805072E-4 46-47 3.9746825274629644E-4 0.0 0.0 0.5752829973959553 0.0010459690861744644 48-49 4.1838763446978573E-4 0.0 0.0 0.7001717062851864 0.001087807849621443 50-51 4.1838763446978573E-4 0.0 0.0 0.8381977868967687 0.0011714853765154 52-53 4.1838763446978573E-4 0.0 0.0 0.9749059464597712 0.0012133241399623785 54-55 4.1838763446978573E-4 0.0 0.0 1.139206770516056 0.0012133241399623785 56-57 4.1838763446978573E-4 0.0 0.0 1.3462458914334294 0.001255162903409357 58-59 4.1838763446978573E-4 0.0 0.0 1.5566530328082848 0.001255162903409357 60-61 4.1838763446978573E-4 0.0 0.0 1.7866616348580495 0.0012970016668563357 62-63 4.1838763446978573E-4 0.0 0.0 2.029995883065677 0.0012970016668563357 64-65 4.1838763446978573E-4 0.0 0.0 2.283329595737132 0.0012970016668563357 66-67 4.1838763446978573E-4 0.0 0.0 2.580698606936532 0.0012970016668563357 68-69 4.1838763446978573E-4 2.0919381723489285E-5 0.0 2.919341558276377 0.0012970016668563357 70-71 4.1838763446978573E-4 4.183876344697857E-5 0.0 3.283840865426454 0.0013806791937502928 72-73 4.1838763446978573E-4 4.183876344697857E-5 0.0 3.653277146663275 0.0014225179571972714 74-75 4.1838763446978573E-4 4.183876344697857E-5 0.0 4.053569515942242 0.0014225179571972714 76-77 4.1838763446978573E-4 4.183876344697857E-5 0.0 4.475680800358809 0.0014434373389207607 78-79 4.1838763446978573E-4 4.183876344697857E-5 0.0 4.982933968389977 0.0014852761023677393 80-81 5.020651613637429E-4 4.183876344697857E-5 0.0 5.529222702717177 0.0015689536292616965 82-83 5.020651613637429E-4 4.183876344697857E-5 0.0 6.051098518573064 0.0016317117744321643 84-85 5.020651613637429E-4 4.183876344697857E-5 0.0 6.612219094542217 0.0017153893013261215 86-87 5.439039248107214E-4 4.183876344697857E-5 0.0 7.203066111940448 0.0017572280647731 88-89 5.857426882577E-4 4.183876344697857E-5 0.0 7.843743096604031 0.0018409055916670572 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 775 0.0 23.269215 3 GTAAGCG 505 0.0 23.023666 94-95 CTTTGCG 835 0.0 22.165522 2 GAGTACT 6315 0.0 21.681328 12-13 GTACATG 6845 0.0 21.501038 1 TTGCGTT 955 0.0 19.877254 4 GTACTTT 6980 0.0 19.785887 14-15 TACATGG 7235 0.0 19.612478 2 ACATGGG 7060 0.0 18.888672 3 AGTACTT 7050 0.0 17.939962 12-13 ACGACCG 135 0.009452507 17.576647 5 CATGGGG 3270 0.0 17.270264 4 AGAGTAC 11775 0.0 16.766457 10-11 GTACCGG 230 4.1756157E-5 16.566721 1 TGGACCG 290 1.0401945E-6 16.364466 5 ACTTTTT 8965 0.0 15.695795 16-17 TATAGGG 470 2.6375346E-10 15.145834 2 CTCCGAC 650 0.0 14.967191 94-95 TAGGACA 1295 0.0 14.658517 4 TACTTTT 8565 0.0 14.628323 14-15 >>END_MODULE