FastQCFastQC Report
Thu 26 May 2016
SRR938910_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938910_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2398838
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT218930.9126502081424422No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT111780.46597560985777275No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT90270.3763071954004397No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC82160.3424991600099715No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT61390.25591557245633095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59420.24770326299650083No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT52330.2181472863111223No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC50450.21031015850174126No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC48980.2041821915444061No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47490.19797085088697114No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG45590.19005034937749027No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA43970.18329707966940662No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC42030.17520983075972615No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA33170.1382752816155155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29990.12501886329964756No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC28010.11676486698976754No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT26830.11184581868387944No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG26010.10842749697978771No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA25720.10721857832834064No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC25090.10459230677519699No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG67550.026.741811
TACATGG70700.024.9689712
ACATGGG69650.024.1222823
TTTGCGT7050.022.8244863
ACCGTAA1150.003803430120.5774928
GAGTACT55900.020.19302412-13
CTTTGCG8700.019.0396942
CATGGGA39850.019.0021534
CATGGGG35950.018.2989884
GTACTTT63550.017.72462814-15
GTATAGG6750.017.5601181
AGAGTAC101300.016.56254410-11
AGTACTT64200.016.55030812-13
TTGCGTT10600.015.626924
AGTACAT50850.015.5430662
TGCACCG4652.3464963E-1015.2668525
GTATAAG9950.015.2481811
ACTTTTT79500.014.407433516-17
GTCTACG2000.005345319414.2236951
GTAAGCG3350.014.20086894-95
Produced by FastQC (version 0.11.5)