FastQCFastQC Report
Thu 26 May 2016
SRR938910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2398838
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT226580.9445406484306152No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT107470.44800857748626627No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT85840.3578399208283344No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC76930.32069693743387423No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT63280.2637943871157619No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60530.2523305033520396No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC53870.22456706121880676No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC53420.22269115296656133No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT53000.22094030526446556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52550.21906439701222008No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG50090.2088094318999449No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA45210.1884662490755941No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC43190.18004550536551447No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA35490.1479466308270921No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30450.12693645840194295No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT28650.1194328253929611No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC28510.1188492094922625No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC26630.11101208168288147No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA25940.10813568902943842No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG25750.10734363887849034No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT6150.025.4618683
GTACATG71300.023.6551061
GTAAGCG3050.023.33737294-95
GTATACG1252.2110059E-422.8692741
TACATGG75000.022.2706472
CTTTGCG7500.021.5114212
ACATGGG76750.021.1445873
ATACGGC1404.887292E-420.3364266
GAGTACT62300.020.03259712-13
GTACTTT66700.018.42652714-15
TTGCGTT9200.017.0207064
AGTACAT56250.016.9560622
CATGGGG35900.016.91874
AGTACTT67050.016.77332112-13
GTACCGT1800.002637573815.8172226
CATGGGA45050.015.5890044
GAGTACA77000.015.2833081
GTATATA11950.015.15049651
AGAGTAC114900.015.13627310-11
GTATAGG7400.014.8083711
Produced by FastQC (version 0.11.5)