##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938910_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2398838 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10571159869904 33.0 31.0 34.0 30.0 34.0 2 32.335269409605814 34.0 31.0 34.0 30.0 34.0 3 32.44087762491673 34.0 31.0 34.0 30.0 34.0 4 36.01349736830915 37.0 35.0 37.0 35.0 37.0 5 35.92479108635097 37.0 35.0 37.0 35.0 37.0 6 35.970571585075774 37.0 35.0 37.0 35.0 37.0 7 35.911522161980095 37.0 35.0 37.0 35.0 37.0 8 35.88044044658289 37.0 35.0 37.0 35.0 37.0 9 37.55132359917594 39.0 37.0 39.0 35.0 39.0 10-11 37.46300959047672 39.0 37.0 39.0 34.0 39.0 12-13 37.396643083026035 39.0 37.0 39.0 34.0 39.0 14-15 38.62791193069312 40.0 38.0 41.0 34.0 41.0 16-17 38.6304093898796 40.0 38.0 41.0 34.0 41.0 18-19 38.73794583043957 40.0 38.0 41.0 34.0 41.0 20-21 38.73054099526521 40.0 38.0 41.0 34.0 41.0 22-23 38.67537657815993 40.0 38.0 41.0 34.0 41.0 24-25 38.60841519935903 40.0 38.0 41.0 34.0 41.0 26-27 38.44490082281504 40.0 38.0 41.0 34.0 41.0 28-29 38.275571964426106 40.0 38.0 41.0 34.0 41.0 30-31 38.14439824615084 40.0 38.0 41.0 33.0 41.0 32-33 37.99957437726099 40.0 37.0 41.0 33.0 41.0 34-35 37.9663751783155 40.0 37.0 41.0 33.0 41.0 36-37 37.86944408084247 40.0 37.0 41.0 33.0 41.0 38-39 37.74944848297384 40.0 37.0 41.0 33.0 41.0 40-41 37.634107013479024 40.0 37.0 41.0 33.0 41.0 42-43 37.478456235894214 40.0 37.0 41.0 32.0 41.0 44-45 37.25308420160094 40.0 37.0 41.0 31.0 41.0 46-47 36.991521103134104 40.0 36.0 41.0 31.0 41.0 48-49 36.739608718888064 39.5 36.0 41.0 30.5 41.0 50-51 36.479210142577365 39.0 36.0 41.0 30.0 41.0 52-53 36.28267123498961 39.0 35.0 40.5 30.0 41.0 54-55 36.05322514484096 39.0 35.0 40.0 29.0 41.0 56-57 35.765579209600645 38.5 35.0 40.0 28.5 41.0 58-59 35.463075038831306 38.0 34.5 40.0 28.0 41.0 60-61 35.02400683164099 38.0 34.0 40.0 27.5 41.0 62-63 34.73238897332792 37.5 34.0 40.0 27.0 41.0 64-65 34.48019020042204 37.0 34.0 39.5 26.0 41.0 66-67 34.00630638667555 36.5 33.0 39.0 26.0 40.5 68-69 33.56869784453973 36.0 33.0 39.0 26.0 40.0 70-71 33.07559660135449 35.0 32.0 38.5 25.0 40.0 72-73 32.536012644455354 35.0 32.0 37.5 23.5 39.5 74-75 32.06427924686869 35.0 31.0 37.0 22.5 39.0 76-77 30.067700069783786 33.0 29.0 35.0 20.0 37.5 78-79 31.070074135894128 34.0 30.5 36.0 20.0 38.0 80-81 31.04675096859396 34.0 31.0 35.5 20.0 37.0 82-83 30.703910184847828 34.0 31.0 35.0 19.5 37.0 84-85 30.28083451237641 34.0 30.0 35.0 18.0 36.5 86-87 29.717509060636857 34.0 29.5 35.0 10.5 36.0 88-89 29.120752631065542 34.0 29.0 35.0 6.5 35.5 90-91 28.626722813295437 33.5 29.0 35.0 2.0 35.0 92-93 28.251615365439434 33.0 29.0 35.0 2.0 35.0 94-95 27.82096039832619 33.0 27.0 35.0 2.0 35.0 96-97 27.140781495040514 33.0 26.5 34.5 2.0 35.0 98-99 26.19050744568829 32.5 25.0 34.0 2.0 35.0 100-101 21.69529726475902 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12475926418065342 1101 2 0.0791544131119366 1101 3 0.07120637547726716 1101 4 0.03402504920444471 1101 5 0.118325242602495 1101 6 0.06264626065478751 1101 7 0.02216298131072847 1101 8 0.10051625244732065 1101 9 0.12626477498318422 1101 10-11 0.14453961319070174 1101 12-13 0.13631981620993372 1101 14-15 0.13931016498980142 1101 16-17 0.10969948546516406 1101 18-19 0.1327655388915545 1101 20-21 0.08929982110378631 1101 22-23 0.12572894576758387 1101 24-25 0.11487200912089435 1101 26-27 0.09522997224239305 1101 28-29 0.178332429671876 1101 30-31 0.15185007831271946 1101 32-33 0.20410664482697172 1101 34-35 0.3927123447759442 1101 36-37 0.2726020826646618 1101 38-39 0.361672304908339 1101 40-41 0.3472101503093228 1101 42-43 0.25902143118825194 1101 44-45 0.2726972132203045 1101 46-47 0.3617669878934464 1101 48-49 0.2220604389650518 1101 50-51 0.26055297268953126 1101 52-53 0.3583509942763925 1101 54-55 0.3688322448562005 1101 56-57 0.19578475073003432 1101 58-59 0.19163097070024548 1101 60-61 0.22887071234219292 1101 62-63 0.2026090737415842 1101 64-65 0.39460257659192877 1101 66-67 0.44012688023111224 1101 68-69 0.33943327152544356 1101 70-71 0.3657483934966663 1101 72-73 0.27108315553034146 1101 74-75 0.21685692788935285 1101 76-77 0.05702916927504731 1101 78-79 0.3869545999243371 1101 80-81 0.46004884359741993 1101 82-83 0.3874962382180698 1101 84-85 0.26330408097809865 1101 86-87 0.34050730753060066 1101 88-89 0.5092532381166635 1101 90-91 0.5320631725543841 1101 92-93 0.6692672019178652 1101 94-95 0.5301771784063938 1101 96-97 0.47040524018254715 1101 98-99 0.5042978564976863 1101 100-101 1.0049452442661284 1102 1 0.07819553979053495 1102 2 0.04448720591386035 1102 3 0.08184808761050277 1102 4 0.0647070437975259 1102 5 0.06661963273908356 1102 6 0.03872895230816198 1102 7 0.018733180816688844 1102 8 0.11437179381928786 1102 9 0.14432029367213772 1102 10-11 0.15701165154912644 1102 12-13 0.1587022003556271 1102 14-15 0.17110881543086975 1102 16-17 0.19130223348825126 1102 18-19 0.17496620152514453 1102 20-21 0.2247920224099076 1102 22-23 0.23080901600449266 1102 24-25 0.1752470349610391 1102 26-27 0.16455453320138957 1102 28-29 0.16111485532266556 1102 30-31 0.14952446605230563 1102 32-33 0.17678768866391437 1102 34-35 0.3050985979039069 1102 36-37 0.23095482123648736 1102 38-39 0.3787527354995248 1102 40-41 0.3649937945688748 1102 42-43 0.19013203018875657 1102 44-45 0.3355319787205673 1102 46-47 0.320248424420015 1102 48-49 0.2365528341224703 1102 50-51 0.1462309816678271 1102 52-53 0.21016997899431544 1102 54-55 0.2868140437479525 1102 56-57 0.22307314112884313 1102 58-59 0.15685839205171703 1102 60-61 0.0671042203025678 1102 62-63 0.1180533109287012 1102 64-65 0.0835172989140247 1102 66-67 0.20133365682192306 1102 68-69 0.19839338400745987 1102 70-71 0.276480475725009 1102 72-73 0.2409040884135507 1102 74-75 0.2610532131837573 1102 76-77 0.0995238262990199 1102 78-79 0.11602408958064458 1102 80-81 0.33529145935117555 1102 82-83 0.3085726196498477 1102 84-85 0.4092608336391308 1102 86-87 0.6647712557747205 1102 88-89 0.700530171180052 1102 90-91 0.7993209650144557 1102 92-93 0.6211383897436811 1102 94-95 0.6973441583721467 1102 96-97 0.5833811310338142 1102 98-99 0.5825265061102627 1102 100-101 1.5050837956580345 1103 1 -0.02157076733543306 1103 2 0.049486736706711554 1103 3 0.09796861960033709 1103 4 0.06940901411116585 1103 5 0.009420906290017683 1103 6 0.013160726619922514 1103 7 0.11967646535379828 1103 8 0.08936879445717238 1103 9 0.059135241938584215 1103 10-11 0.12309935883672551 1103 12-13 0.07896569283267496 1103 14-15 0.09429891321997275 1103 16-17 0.1717858890434769 1103 18-19 0.13249850059658996 1103 20-21 0.16877987529030491 1103 22-23 0.1847576217861544 1103 24-25 0.17998276047208606 1103 26-27 0.109492877007078 1103 28-29 0.17884425307055096 1103 30-31 0.14707676879029918 1103 32-33 0.2444057833503095 1103 34-35 0.10362461435110504 1103 36-37 0.17762754866038932 1103 38-39 0.2401010348908983 1103 40-41 0.21820214194212184 1103 42-43 0.19997884692515555 1103 44-45 0.13238597452422596 1103 46-47 0.2608251215739301 1103 48-49 0.1910819105129562 1103 50-51 0.2616677184885461 1103 52-53 0.3498495798079233 1103 54-55 0.2822276924095988 1103 56-57 0.2308731654405065 1103 58-59 0.10522739238709278 1103 60-61 0.2373004993513348 1103 62-63 0.343010716027095 1103 64-65 0.31735479565234215 1103 66-67 0.37672892919369616 1103 68-69 0.3227698037550226 1103 70-71 0.29269672993154217 1103 72-73 0.21921444379540134 1103 74-75 0.25750307711368947 1103 76-77 0.18822981239227587 1103 78-79 0.47844859573517695 1103 80-81 0.3746452680659189 1103 82-83 0.5362115336709081 1103 84-85 0.5071872350341593 1103 86-87 0.489207999326684 1103 88-89 0.5833876585183866 1103 90-91 0.7531980018585003 1103 92-93 0.567946335934927 1103 94-95 0.6493604028381732 1103 96-97 0.4949289523486442 1103 98-99 0.5183236256931494 1103 100-101 0.9768234692403261 1104 1 0.0031655807722899 1104 2 -0.035345570423906736 1104 3 0.025452481704007823 1104 4 0.05681497125841872 1104 5 0.06064189068818138 1104 6 -0.011768203342803929 1104 7 0.005490669790049196 1104 8 -0.07068158557358828 1104 9 0.04435911930986691 1104 10-11 -0.021422034730342432 1104 12-13 0.04094177563283807 1104 14-15 0.05845939140959899 1104 16-17 0.060770361311305976 1104 18-19 0.06291576256889897 1104 20-21 -0.003058810583603133 1104 22-23 -0.037969468498850745 1104 24-25 -0.027365375806404302 1104 26-27 0.08424014709174088 1104 28-29 0.03993648549948148 1104 30-31 0.07212180747261954 1104 32-33 0.08728639109078529 1104 34-35 0.059317858857497185 1104 36-37 0.019022304413347513 1104 38-39 0.1437080274261362 1104 40-41 0.18327535778122694 1104 42-43 0.05928709213009853 1104 44-45 0.011059405869765726 1104 46-47 -0.14557720237576177 1104 48-49 -0.17934571768905272 1104 50-51 -0.1834965279760823 1104 52-53 -0.267816196891296 1104 54-55 -0.1886553011846388 1104 56-57 -0.10997935082089327 1104 58-59 -0.1128524545971672 1104 60-61 -0.0731307412077129 1104 62-63 -0.11922992561396484 1104 64-65 -0.19400222717883508 1104 66-67 -0.23251862764110598 1104 68-69 -0.1904797506823499 1104 70-71 -0.3399136302775716 1104 72-73 -0.3559195916319453 1104 74-75 -0.1728967212735597 1104 76-77 -0.24943995102149685 1104 78-79 -0.11441310116182635 1104 80-81 -0.08852924119769412 1104 82-83 0.020414321978297068 1104 84-85 -0.14908425675351467 1104 86-87 -0.12329212610238116 1104 88-89 -0.061174547736392526 1104 90-91 0.12911585315443475 1104 92-93 0.002311236483187429 1104 94-95 -0.0329899139763441 1104 96-97 -0.0074734001602827504 1104 98-99 0.1397878768662011 1104 100-101 0.5738495075421177 1105 1 -0.06390545260931901 1105 2 -0.0597483033339401 1105 3 0.04715980783375073 1105 4 0.006333741448905528 1105 5 -0.01057269473382405 1105 6 0.04533364954048835 1105 7 0.051199703988558554 1105 8 0.07510171248441111 1105 9 -0.07662524814770677 1105 10-11 0.008739303504405882 1105 12-13 0.02856867400274865 1105 14-15 0.08115776874426928 1105 16-17 0.027958360271611582 1105 18-19 -0.012965378123986682 1105 20-21 0.12629490817916889 1105 22-23 -0.007523142703298902 1105 24-25 0.08172563455341475 1105 26-27 0.07299659876454001 1105 28-29 0.03537798414416926 1105 30-31 0.022582429061124287 1105 32-33 -0.0946054807297898 1105 34-35 -0.011777533070016943 1105 36-37 0.0709553926759412 1105 38-39 0.09026552621934769 1105 40-41 0.2062715331924636 1105 42-43 0.17383705314157538 1105 44-45 0.17281611746606984 1105 46-47 0.1809528187894358 1105 48-49 0.13677455263140104 1105 50-51 0.20773272455816993 1105 52-53 0.3099194980327695 1105 54-55 0.22040915984539566 1105 56-57 0.1306422376830696 1105 58-59 0.14351604267363172 1105 60-61 0.055471427296986064 1105 62-63 0.02264635079612276 1105 64-65 -0.106759087302855 1105 66-67 0.06206605172467761 1105 68-69 0.022821277872104417 1105 70-71 0.06411852597359058 1105 72-73 -0.11243197513051228 1105 74-75 0.04819087294870883 1105 76-77 -0.1877740269934236 1105 78-79 0.01439682048980373 1105 80-81 0.00771950559967749 1105 82-83 -0.008200842701814537 1105 84-85 -0.06582592991827241 1105 86-87 -0.17032769849107865 1105 88-89 -0.09762065074036386 1105 90-91 0.22624852553941466 1105 92-93 0.19871129589427383 1105 94-95 0.19123718490598307 1105 96-97 0.21083765401520438 1105 98-99 0.34464826081035227 1105 100-101 0.7317740183289452 1106 1 0.0337235965500966 1106 2 -0.04630987789314389 1106 3 0.002005131083102185 1106 4 0.012518077865145472 1106 5 -0.03839993059877855 1106 6 0.055847112568415014 1106 7 0.021992906412357627 1106 8 0.0034556238243297344 1106 9 -0.01770593519495378 1106 10-11 0.10406938687949463 1106 12-13 0.11371514992419662 1106 14-15 0.04014861115166468 1106 16-17 0.03369620709835175 1106 18-19 -0.06420370333243142 1106 20-21 0.004592324917972235 1106 22-23 0.13751307762564835 1106 24-25 0.07936242894039935 1106 26-27 0.09113182132593067 1106 28-29 0.20573699822225677 1106 30-31 0.09500850769796898 1106 32-33 0.10917464592746029 1106 34-35 0.08414847722657726 1106 36-37 0.05847691985258052 1106 38-39 0.27097034192848213 1106 40-41 0.2509801759552346 1106 42-43 0.1786422834481911 1106 44-45 0.2152904898748318 1106 46-47 0.11259571823815406 1106 48-49 0.07786799652405563 1106 50-51 0.07569800771457125 1106 52-53 0.2673731405677344 1106 54-55 0.2691307416942337 1106 56-57 0.20798425782065522 1106 58-59 0.2781763916644735 1106 60-61 0.27126303511510486 1106 62-63 0.20106541316798854 1106 64-65 0.3274916758298616 1106 66-67 0.20243508903845253 1106 68-69 0.2065339754893074 1106 70-71 0.18995751636038705 1106 72-73 0.3377703119896225 1106 74-75 0.4611053565242642 1106 76-77 0.1540957620630259 1106 78-79 0.31114063569778594 1106 80-81 0.410307322812308 1106 82-83 0.43570958601931054 1106 84-85 0.32317265771670023 1106 86-87 0.3052137840365994 1106 88-89 0.5358100294728558 1106 90-91 0.7179237727032977 1106 92-93 0.5723638540973788 1106 94-95 0.4203893102875469 1106 96-97 0.3571454584709848 1106 98-99 0.48246410663476524 1106 100-101 0.8564033864094931 1107 1 -0.1374907123859046 1107 2 -0.10229932926647933 1107 3 -0.09237965041612028 1107 4 -0.09045798612404354 1107 5 -0.2351185594226095 1107 6 -0.1019491180411265 1107 7 -0.16800914592030836 1107 8 -0.044000664214202345 1107 9 -0.06900898481600137 1107 10-11 0.01953189730007665 1107 12-13 -0.0821759166167837 1107 14-15 0.007791952058653351 1107 16-17 -0.12939767531570823 1107 18-19 -0.10247253348464369 1107 20-21 -0.08197751886864069 1107 22-23 -0.14521826839582275 1107 24-25 -0.22186088846465424 1107 26-27 -0.20285202881382958 1107 28-29 -0.14181473236639874 1107 30-31 -0.18104830740865197 1107 32-33 -0.10399515463302578 1107 34-35 -0.31033274574574676 1107 36-37 -0.09228752866020784 1107 38-39 -0.09089268575257137 1107 40-41 -0.22678352608965469 1107 42-43 -0.16467755534380046 1107 44-45 -0.18801004246030573 1107 46-47 -0.3933923709674829 1107 48-49 -0.2972550072191851 1107 50-51 -0.2547239191896935 1107 52-53 -0.14943119584772546 1107 54-55 -0.3315591508398157 1107 56-57 -0.24319855779814503 1107 58-59 -0.21326810545045305 1107 60-61 -0.35101676139520066 1107 62-63 -0.26020261966207414 1107 64-65 -0.3711584159802399 1107 66-67 -0.13277685517441995 1107 68-69 -0.2390744866596819 1107 70-71 -0.10424936603731538 1107 72-73 -0.17895102300736454 1107 74-75 -0.36335118396934263 1107 76-77 -0.5815859483837116 1107 78-79 -0.4153225594390939 1107 80-81 -0.1356602067421946 1107 82-83 -0.1431244165924177 1107 84-85 -0.31976976981144034 1107 86-87 -0.08169651086362606 1107 88-89 -0.24894903938562862 1107 90-91 -0.002970971690476887 1107 92-93 0.05716959990236248 1107 94-95 -0.03621720305351772 1107 96-97 -0.09600514460606036 1107 98-99 -0.29358753223459644 1107 100-101 0.6596768570130074 1108 1 0.04367385356474074 1108 2 0.05057625180011627 1108 3 0.006728778090497656 1108 4 0.001519842938819238 1108 5 -0.025569670928227595 1108 6 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2316 52-53 -1.3158466306991983 2316 54-55 -1.3244117726905884 2316 56-57 -1.3574096542732335 2316 58-59 -1.330127192679626 2316 60-61 -1.1782776324964317 2316 62-63 -1.2900129630206294 2316 64-65 -1.2969491393586523 2316 66-67 -1.404523213908817 2316 68-69 -1.458472818747616 2316 70-71 -1.4435085787859059 2316 72-73 -1.3294831149215476 2316 74-75 -1.6690824162476687 2316 76-77 -1.7427930545405452 2316 78-79 -1.7988730876864558 2316 80-81 -1.83041080517118 2316 82-83 -2.048537827636167 2316 84-85 -2.3126707083274063 2316 86-87 -2.295935127798714 2316 88-89 -2.3763219368685036 2316 90-91 -2.747075076810912 2316 92-93 -3.041639019493836 2316 94-95 -3.215333760662574 2316 96-97 -3.2636288671832006 2316 98-99 -3.774826712938335 2316 100-101 -4.289515312095023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 9.0 4 11.0 5 54.0 6 126.0 7 261.0 8 626.0 9 1229.0 10 2028.0 11 3189.0 12 4549.0 13 6198.0 14 7351.0 15 9762.0 16 12394.0 17 16858.0 18 16658.0 19 12538.0 20 12350.0 21 13158.0 22 14299.0 23 16400.0 24 19128.0 25 22742.0 26 27703.0 27 33934.0 28 41381.0 29 50171.0 30 61404.0 31 74262.0 32 92396.0 33 116398.0 34 156821.0 35 220942.0 36 350085.0 37 545896.0 38 408663.0 39 26861.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.43151182270896 20.009734551421744 14.418477735457078 23.14027589041222 2 15.373484995652062 21.924239986193314 38.75067845348456 23.951596564670062 3 20.253222601943108 24.57181351971246 30.40972337440044 24.765240503943993 4 13.056779990978965 17.075809204289744 37.751694778888776 32.11571602584251 5 13.163725437474232 37.92403537228821 34.88859346672353 14.023645723514033 6 31.226076958927614 36.88873529600581 17.08514705870092 14.800040686365648 7 27.35411895259288 33.04095566270002 21.613547892771418 17.99137749193568 8 25.041874440875123 34.93366371551559 20.156092241326842 19.86836960228244 9 25.087313107429516 17.738171564732593 21.651774734267175 35.522740593570724 10-11 24.666526043025833 26.34719393306259 27.817530821172586 21.168749202738994 12-13 26.185094616643557 24.078345432246778 27.758731519177203 21.977828431932462 14-15 22.432419081215077 25.709440453691716 27.040767514052284 24.817372951040923 16-17 21.574727783101093 28.75501323278763 27.537204904250146 22.13305407986113 18-19 21.3733482627839 28.574772452329 28.756026876345963 21.295852408541137 20-21 22.590979465891404 26.826551021786383 28.293698865867555 22.288770646454658 22-23 21.848828474453047 26.911133640537628 28.61791834213065 22.62211954287868 24-25 22.702220825249558 26.993548543086277 28.389015848506656 21.915214783157513 26-27 22.370581089677586 26.48694909785488 29.45323944343053 21.68923036903701 28-29 21.448926521924367 28.153505989149746 28.675154387249158 21.72241310167673 30-31 22.00717597436759 26.930205374435456 28.44960768505418 22.613010966142774 32-33 22.045486189563444 27.160170882735724 28.73189019016707 22.062452737533754 34-35 22.850788423257704 26.8355306354285 28.50281914727309 21.810861794040708 36-37 22.476233674786872 26.549892085896044 29.16275998294052 21.811114256376563 38-39 21.909153087339554 27.367059692198907 29.010274837145012 21.713512383316527 40-41 22.050333597287448 27.442584768770566 28.533078647835115 21.974002986106868 42-43 21.69748042787704 27.549440975146112 27.88131247863533 22.87176611834152 44-45 22.287798158310892 26.728045187019255 28.093127046700467 22.891029607969386 46-47 22.838303296089443 27.501227162019777 27.919639907413103 21.740829634477677 48-49 22.51612037211505 27.31696478446975 28.007135150998735 22.159779692416464 50-51 21.480095305251314 27.540349551247832 28.76561940805442 22.21393573544643 52-53 22.343713914820427 28.094289818653866 27.296278448148648 22.265717818377066 54-55 21.780149388995838 27.170905246623573 27.937026176840618 23.111919187539968 56-57 22.45966588823422 27.168987651521277 27.70220415050954 22.669142309734962 58-59 23.212978157481324 26.996530184187666 27.469198011280916 22.321293647050094 60-61 22.327453758351364 27.329111889792518 28.52619733035627 21.817237021499846 62-63 21.816250201139052 27.36314415562868 28.348975629033724 22.47163001419854 64-65 21.648313649113206 28.097698318787028 27.809891766143835 22.44409626595593 66-67 22.056687962744682 27.315424784532755 27.285597352174435 23.34228990054813 68-69 21.634909303137523 27.369368899912978 27.817178470373054 23.17854332657645 70-71 22.52384279388604 27.213675954774768 27.448143642880428 22.81433760845876 72-73 22.278182186541983 26.523049909998093 28.355645525041705 22.84312237841822 74-75 21.6404359110536 28.122449285862572 27.44462110405121 22.792493699032615 76-77 21.904332199653197 27.034462637910966 27.432687176698213 23.628517985737627 78-79 21.978810135167667 27.035451159531366 27.836193853225865 23.149544852075103 80-81 22.39486367983165 26.928537900433458 27.565179474395517 23.111418945339366 82-83 22.00832236274396 26.765917498388802 28.15010851087068 23.075651627996553 84-85 21.76453766365215 27.10022519236397 27.84164666392645 23.29359048005743 86-87 21.70727832875042 27.17884682543112 27.71042739988298 23.403447445935484 88-89 21.595584497588582 27.12367191994714 27.19839277923074 24.082350803233535 90-91 21.868067446406727 26.362207892896905 27.52558541679869 24.24413924389768 92-93 22.226761457005434 26.947859755431587 27.591233755676708 23.23414503188627 94-95 21.945291845468514 26.556295172912886 27.672335522448787 23.826077459169813 96-97 21.056280297404122 26.52120925111831 28.387103094496112 24.03540735698145 98-99 21.555207146126584 26.913134609340023 27.219220305831403 24.31243793870199 100-101 21.4801411687021 25.81072788757195 28.068722611671014 24.640408332054932 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 629.0 1 427.5 2 258.0 3 306.0 4 292.0 5 292.5 6 442.5 7 860.0 8 1399.5 9 1680.0 10 1599.5 11 1589.0 12 1755.0 13 1859.0 14 1953.5 15 2050.0 16 2244.0 17 2463.5 18 2654.5 19 3024.0 20 3541.5 21 4076.5 22 4937.5 23 6136.0 24 7654.5 25 9706.0 26 12759.5 27 16097.0 28 18912.5 29 22274.0 30 26301.0 31 30869.5 32 35489.5 33 40408.0 34 46004.5 35 51394.0 36 57585.0 37 63333.5 38 67449.0 39 69512.5 40 70489.5 41 72414.5 42 74250.0 43 77279.0 44 80988.5 45 83639.5 46 86828.5 47 104344.5 48 137217.5 49 134190.5 50 103222.0 51 90693.5 52 85826.5 53 80881.0 54 75516.0 55 68854.5 56 62171.0 57 55082.0 58 48318.0 59 41731.5 60 34847.5 61 27805.0 62 21715.0 63 16809.5 64 12373.0 65 8846.0 66 6314.5 67 4744.0 68 3356.0 69 2147.0 70 1442.5 71 986.0 72 597.0 73 367.0 74 257.5 75 171.0 76 102.5 77 54.0 78 24.0 79 9.5 80 6.0 81 6.5 82 5.0 83 2.5 84 1.5 85 0.5 86 1.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39231494581960097 2 0.0 3 0.0 4 0.0 5 4.585553505488907E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001188075226422126 16-17 0.0025637412780687985 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002626271553143647 36-37 0.0071284513585327565 38-39 0.012860393240393892 40-41 0.0026054281281186974 42-43 7.503633008981848E-4 44-45 3.126513753742437E-4 46-47 4.3771192552394115E-4 48-49 4.168685004989916E-4 50-51 8.128935759730336E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.126513753742437E-4 60-61 0.002230246477669605 62-63 0.0 64-65 1.875908252245462E-4 66-67 0.0016466305769710168 68-69 0.0021051859275199075 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12312211162237717 78-79 1.042171251247479E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14709205040106918 88-89 0.4425476001297295 90-91 0.34056072148265115 92-93 0.0 94-95 0.0 96-97 6.044593257235378E-4 98-99 0.0 100-101 2.50121100299395E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2398838.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.760136296989444 #Duplication Level Percentage of deduplicated Percentage of total 1 72.95175234872661 38.48944397023036 2 14.556016391165622 15.359548174782214 3 5.371646102792487 8.502263415675715 4 2.541437025183312 5.363462553555479 5 1.3281703187434202 3.5037223521259384 6 0.8131698442481476 2.574177108908037 7 0.546426915764657 2.018069098646081 8 0.36708745276969357 1.549406723283497 9 0.27124346364044405 1.2879757900203597 >10 1.154578547720471 10.340857587624335 >50 0.06523330656411301 2.3405834999804607 >100 0.029760798823009166 2.708216526153932 >500 0.00110647213661167 0.42102774041135693 >1k 0.0015016407568294986 1.856950417325266 >5k 7.113035163929203E-4 2.2913633122053465 >10k+ 1.5806744808731564E-4 1.392931729071711 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22658 0.9445406484306152 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10747 0.44800857748626627 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8584 0.3578399208283344 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7693 0.32069693743387423 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6328 0.2637943871157619 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6053 0.2523305033520396 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5387 0.22456706121880676 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5342 0.22269115296656133 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5300 0.22094030526446556 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5255 0.21906439701222008 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5009 0.2088094318999449 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4521 0.1884662490755941 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4319 0.18004550536551447 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3549 0.1479466308270921 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3045 0.12693645840194295 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2865 0.1194328253929611 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2851 0.1188492094922625 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2663 0.11101208168288147 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2594 0.10813568902943842 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2575 0.10734363887849034 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.168685004989916E-5 2 0.0 0.0 0.0 0.0 4.168685004989916E-5 3 0.0 0.0 0.0 0.0 4.168685004989916E-5 4 0.0 0.0 0.0 0.0 4.168685004989916E-5 5 0.0 0.0 0.0 0.0 4.168685004989916E-5 6 0.0 0.0 0.0 0.0 4.168685004989916E-5 7 4.168685004989916E-5 0.0 0.0 0.0 4.168685004989916E-5 8 4.168685004989916E-5 0.0 0.0 0.0 4.168685004989916E-5 9 4.168685004989916E-5 0.0 0.0 0.0 8.337370009979831E-5 10-11 4.168685004989916E-5 0.0 0.0 4.168685004989916E-5 8.337370009979831E-5 12-13 8.337370009979831E-5 0.0 0.0 6.253027507484874E-5 1.6674740019959663E-4 14-15 1.2506055014969747E-4 0.0 0.0 1.2506055014969747E-4 1.6674740019959663E-4 16-17 1.2506055014969747E-4 0.0 0.0 2.0843425024949578E-4 1.6674740019959663E-4 18-19 1.2506055014969747E-4 0.0 0.0 2.918079503492941E-4 1.6674740019959663E-4 20-21 1.2506055014969747E-4 0.0 0.0 4.793987755738403E-4 2.0843425024949578E-4 22-23 1.2506055014969747E-4 0.0 0.0 5.627724756736386E-4 2.2927767527444536E-4 24-25 1.6674740019959663E-4 0.0 0.0 8.337370009979831E-4 2.5012110029939494E-4 26-27 2.0843425024949578E-4 0.0 0.0 0.0016049437269211177 3.126513753742437E-4 28-29 2.0843425024949578E-4 0.0 0.0 0.005523507631611638 3.3349480039919326E-4 30-31 2.0843425024949578E-4 0.0 0.0 0.01650799261976007 3.5433822542414283E-4 32-33 2.0843425024949578E-4 0.0 0.0 0.04008190632297805 4.1686850049899157E-4 34-35 2.0843425024949578E-4 0.0 0.0 0.07326463896269778 4.585553505488908E-4 36-37 2.5012110029939494E-4 0.0 0.0 0.12518561069984718 5.002422005987899E-4 38-39 2.5012110029939494E-4 0.0 0.0 0.200909773815489 5.002422005987899E-4 40-41 2.5012110029939494E-4 0.0 0.0 0.2813862378368194 5.002422005987899E-4 42-43 2.5012110029939494E-4 0.0 0.0 0.3645098168363182 5.002422005987899E-4 44-45 2.5012110029939494E-4 0.0 0.0 0.473354182316605 5.002422005987899E-4 46-47 2.5012110029939494E-4 0.0 0.0 0.6069605367265318 6.044593257235378E-4 48-49 2.5012110029939494E-4 0.0 0.0 0.7402333963360594 6.253027507484874E-4 50-51 2.918079503492941E-4 0.0 0.0 0.8839488118830867 6.669896007983865E-4 52-53 2.918079503492941E-4 0.0 0.0 1.027726757705189 6.669896007983865E-4 54-55 2.918079503492941E-4 0.0 0.0 1.1907431848253196 6.669896007983865E-4 56-57 2.918079503492941E-4 0.0 0.0 1.4018453934780088 7.295198758732352E-4 58-59 2.918079503492941E-4 0.0 0.0 1.6212641287156533 7.503633008981848E-4 60-61 2.918079503492941E-4 0.0 0.0 1.855773503671361 7.503633008981848E-4 62-63 2.918079503492941E-4 0.0 0.0 2.1014549544404417 8.337370009979831E-4 64-65 2.918079503492941E-4 0.0 0.0 2.360809692025889 8.337370009979831E-4 66-67 2.918079503492941E-4 0.0 0.0 2.6694799732203673 8.337370009979831E-4 68-69 2.918079503492941E-4 0.0 0.0 3.0185656555382234 8.337370009979831E-4 70-71 2.918079503492941E-4 0.0 0.0 3.391433685809546 8.337370009979831E-4 72-73 2.918079503492941E-4 0.0 0.0 3.761487853702501 8.96267276072832E-4 74-75 2.918079503492941E-4 0.0 0.0 4.1649123450604 9.171107010977816E-4 76-77 3.3349480039919326E-4 0.0 0.0 4.588283994167176 9.796409761726303E-4 78-79 3.5433822542414283E-4 2.084342502494958E-5 0.0 5.1042421372347775 0.001042171251247479 80-81 4.1686850049899157E-4 4.168685004989916E-5 0.0 5.6536122906173745 0.0010630146762724284 82-83 5.002422005987899E-4 4.168685004989916E-5 0.0 6.182576730900545 0.001188075226422126 84-85 5.210856256237395E-4 4.168685004989916E-5 0.0 6.7463705344004055 0.0012089186514470756 86-87 5.41929050648689E-4 4.168685004989916E-5 0.0 7.338157057708774 0.001229762076472025 88-89 5.41929050648689E-4 4.168685004989916E-5 0.0 7.988284327661976 0.0012714489265219244 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 615 0.0 25.461868 3 GTACATG 7130 0.0 23.655106 1 GTAAGCG 305 0.0 23.337372 94-95 GTATACG 125 2.2110059E-4 22.869274 1 TACATGG 7500 0.0 22.270647 2 CTTTGCG 750 0.0 21.511421 2 ACATGGG 7675 0.0 21.144587 3 ATACGGC 140 4.887292E-4 20.336426 6 GAGTACT 6230 0.0 20.032597 12-13 GTACTTT 6670 0.0 18.426527 14-15 TTGCGTT 920 0.0 17.020706 4 AGTACAT 5625 0.0 16.956062 2 CATGGGG 3590 0.0 16.9187 4 AGTACTT 6705 0.0 16.773321 12-13 GTACCGT 180 0.0026375738 15.817222 6 CATGGGA 4505 0.0 15.589004 4 GAGTACA 7700 0.0 15.283308 1 GTATATA 1195 0.0 15.1504965 1 AGAGTAC 11490 0.0 15.136273 10-11 GTATAGG 740 0.0 14.808371 1 >>END_MODULE