##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938909_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 956720 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.914375156785685 33.0 31.0 34.0 30.0 34.0 2 31.944996446191155 33.0 31.0 34.0 30.0 34.0 3 31.985544359896313 34.0 31.0 34.0 30.0 34.0 4 35.499790952420774 37.0 35.0 37.0 33.0 37.0 5 35.56802826323271 37.0 35.0 37.0 33.0 37.0 6 35.705358934693535 37.0 35.0 37.0 33.0 37.0 7 35.65443912534493 37.0 35.0 37.0 33.0 37.0 8 35.65614286311565 37.0 35.0 37.0 33.0 37.0 9 37.23419913872397 39.0 37.0 39.0 34.0 39.0 10-11 37.13530029684756 39.0 37.0 39.0 33.5 39.0 12-13 37.069990174763774 39.0 37.0 39.0 33.0 39.0 14-15 38.165313780416426 40.0 38.0 41.0 33.0 41.0 16-17 38.13519315996321 40.0 38.0 41.0 33.0 41.0 18-19 38.1607931265156 40.0 38.0 41.0 33.0 41.0 20-21 38.26324211890626 40.0 38.0 41.0 34.0 41.0 22-23 38.217361401454966 40.0 38.0 41.0 33.5 41.0 24-25 38.107367359310985 40.0 38.0 41.0 33.0 41.0 26-27 37.943552972656576 40.0 38.0 41.0 33.0 41.0 28-29 37.8433162262731 40.0 38.0 41.0 33.0 41.0 30-31 37.67190452797057 40.0 37.0 41.0 33.0 41.0 32-33 37.41604335646793 40.0 36.5 41.0 32.0 41.0 34-35 37.052073229367004 40.0 36.0 41.0 31.0 41.0 36-37 36.73542990634668 39.5 35.5 41.0 30.5 41.0 38-39 36.46250313571369 39.0 35.0 41.0 30.0 41.0 40-41 36.369070888034116 39.0 35.0 41.0 30.0 41.0 42-43 36.12347343005268 39.0 35.0 41.0 30.0 41.0 44-45 35.959370557738936 39.0 35.0 41.0 29.5 41.0 46-47 35.618888494021235 39.0 35.0 41.0 27.0 41.0 48-49 35.27470785600803 39.0 35.0 41.0 25.0 41.0 50-51 34.10710343674221 38.0 34.0 40.0 23.5 40.5 52-53 34.218813236892714 38.0 34.0 39.5 23.0 40.5 54-55 34.75146124257881 39.0 35.0 40.5 23.0 41.0 56-57 34.660053620704076 39.0 35.0 41.0 23.0 41.0 58-59 34.496117463834764 38.5 34.5 40.5 22.5 41.0 60-61 34.099662388159544 38.0 34.0 40.0 22.0 41.0 62-63 33.769887741449956 38.0 34.0 40.0 20.5 41.0 64-65 33.36886236307384 37.0 33.0 40.0 18.5 41.0 66-67 32.86339994982858 36.5 33.0 39.0 15.5 41.0 68-69 32.42286562421607 36.0 32.0 39.0 12.5 40.5 70-71 31.8837580483318 35.5 31.5 38.5 8.0 40.0 72-73 31.39869815620035 35.0 31.0 37.5 6.5 40.0 74-75 30.95290628397023 35.0 31.0 37.0 2.0 39.0 76-77 30.513174701061963 35.0 31.0 36.5 2.0 39.0 78-79 30.076585625888452 35.0 30.0 36.0 2.0 38.5 80-81 29.604144890877166 34.0 30.0 36.0 2.0 37.0 82-83 29.129370139643783 34.0 29.0 35.0 2.0 37.0 84-85 28.685435132536163 34.0 29.0 35.0 2.0 36.5 86-87 28.30402416590016 34.0 29.0 35.0 2.0 36.0 88-89 27.758997407810018 34.0 27.5 35.0 2.0 36.0 90-91 27.22937588845221 34.0 26.0 35.0 2.0 35.0 92-93 26.763021573710176 33.5 25.0 35.0 2.0 35.0 94-95 26.21021197424534 33.0 24.5 35.0 2.0 35.0 96-97 25.517468538339326 33.0 20.5 35.0 2.0 35.0 98-99 24.76415879254118 32.5 10.0 35.0 2.0 35.0 100-101 21.703697006438667 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31509823639418144 1101 2 0.44329223278187513 1101 3 0.4350935945129848 1101 4 0.3666363443291445 1101 5 0.12893482329620554 1101 6 0.3348807407699397 1101 7 0.39224839748874984 1101 8 0.4091481002277533 1101 9 0.49077391471305987 1101 10-11 0.33754349018189345 1101 12-13 0.41480145403131274 1101 14-15 0.5893372042758571 1101 16-17 0.5059389159812042 1101 18-19 0.6031874155358778 1101 20-21 0.5158963437791968 1101 22-23 0.6794136613756407 1101 24-25 0.7384049308667002 1101 26-27 0.6756188776936796 1101 28-29 0.5179698377001642 1101 30-31 0.632823653993718 1101 32-33 0.5219241372789227 1101 34-35 0.5691639746001655 1101 36-37 0.6110415607995563 1101 38-39 0.8067514040641512 1101 40-41 0.6221280664825883 1101 42-43 0.7161776747144657 1101 44-45 0.7071817109055232 1101 46-47 0.7219501666610384 1101 48-49 0.7590701449487725 1101 50-51 1.0147069748549455 1101 52-53 0.9388036597073324 1101 54-55 1.1809137113742025 1101 56-57 1.1345340193079068 1101 58-59 1.1071166893381559 1101 60-61 0.9994688089386656 1101 62-63 0.9340517604486038 1101 64-65 0.911953600598622 1101 66-67 0.7635199093900482 1101 68-69 0.7069940432112816 1101 70-71 0.7504167365724328 1101 72-73 0.699204117973661 1101 74-75 0.5981817947671608 1101 76-77 0.24290102357180032 1101 78-79 0.49276304444310526 1101 80-81 0.5583067924759852 1101 82-83 0.6846274578464673 1101 84-85 0.7960348697664301 1101 86-87 0.48472698467711695 1101 88-89 0.220086093092295 1101 90-91 0.27929243221153044 1101 92-93 0.35553832501986093 1101 94-95 0.40024747378656755 1101 96-97 -0.13635016776315467 1101 98-99 -0.353796252637391 1101 100-101 -0.18286914388350084 1102 1 0.1626674436196076 1102 2 0.17391147689960107 1102 3 0.25887683174414633 1102 4 0.18810356483579938 1102 5 0.11655813923094627 1102 6 0.22621241622699273 1102 7 0.231949979758582 1102 8 0.28549876838832944 1102 9 0.34836826335897797 1102 10-11 0.39343396103697614 1102 12-13 0.29797241569064425 1102 14-15 0.40029080195846944 1102 16-17 0.5642738434263421 1102 18-19 0.5452801655153792 1102 20-21 0.5621934497830736 1102 22-23 0.5224751758729411 1102 24-25 0.4393456845627597 1102 26-27 0.43813015034145764 1102 28-29 0.246555906073894 1102 30-31 0.42058292489252125 1102 32-33 0.5273691677492351 1102 34-35 0.6581283940246436 1102 36-37 0.6942459988068279 1102 38-39 0.7891080023206456 1102 40-41 0.6356354312791339 1102 42-43 0.720522084847552 1102 44-45 0.7308207948833285 1102 46-47 0.8278597668577916 1102 48-49 0.6790623565739438 1102 50-51 0.669433288630799 1102 52-53 0.7116229147561128 1102 54-55 0.698715613377324 1102 56-57 0.45819496539049 1102 58-59 0.3611195040841437 1102 60-61 0.5614123500530823 1102 62-63 0.6350396543080805 1102 64-65 0.8474173504961442 1102 66-67 0.8180159878750004 1102 68-69 0.9961231116123486 1102 70-71 0.8250122876341095 1102 72-73 0.706389235345835 1102 74-75 0.6537764440170157 1102 76-77 0.7939633852256698 1102 78-79 0.5688110590907165 1102 80-81 0.3661265940773859 1102 82-83 0.5000443408568778 1102 84-85 0.7325616645085944 1102 86-87 0.4497585480908839 1102 88-89 0.16499563863379407 1102 90-91 0.36118582712312275 1102 92-93 0.42050293573775477 1102 94-95 0.17793992862765506 1102 96-97 0.2253809010204897 1102 98-99 0.07618215853414156 1102 100-101 0.04153579695217857 1103 1 0.14137865042832587 1103 2 0.2628445919699338 1103 3 0.177007830297363 1103 4 0.23428656613315013 1103 5 0.04616832242243163 1103 6 0.08483369143534958 1103 7 0.08435755194498995 1103 8 -0.03874812238281322 1103 9 0.30142722833048197 1103 10-11 0.10166501189180366 1103 12-13 0.12053139085077902 1103 14-15 0.23216218075118888 1103 16-17 0.09794725043476404 1103 18-19 0.0783184815393696 1103 20-21 0.30709811821124333 1103 22-23 0.11156179956625323 1103 24-25 0.1962722517431601 1103 26-27 0.34986608834027777 1103 28-29 0.24518451709255373 1103 30-31 0.3014921578248746 1103 32-33 0.2736044706570695 1103 34-35 0.053936793527441296 1103 36-37 -0.00784538637076082 1103 38-39 0.1011088445803523 1103 40-41 0.006524490733163191 1103 42-43 0.10082950963852255 1103 44-45 0.16625887181425014 1103 46-47 0.3268769041257471 1103 48-49 0.5541803748183725 1103 50-51 0.39256690226454083 1103 52-53 0.5279050174167068 1103 54-55 0.5054163326942742 1103 56-57 0.49738856965069544 1103 58-59 0.45146068718732835 1103 60-61 0.4703654348292403 1103 62-63 0.3593306957893745 1103 64-65 0.27445864159875555 1103 66-67 0.15512160316608714 1103 68-69 0.05455419914622439 1103 70-71 0.1014887892676839 1103 72-73 -0.09919956531710383 1103 74-75 0.14852794344305664 1103 76-77 0.22513989976483728 1103 78-79 0.14201361575645777 1103 80-81 -0.06285868675509221 1103 82-83 0.1330412231375 1103 84-85 0.05962876679560125 1103 86-87 0.03209894327370222 1103 88-89 0.11570714430886042 1103 90-91 0.013594757792922962 1103 92-93 0.14867584350352558 1103 94-95 0.12920297855871254 1103 96-97 -0.1402250165331509 1103 98-99 0.061353633099937355 1103 100-101 0.24489668485692206 1104 1 0.26541725511862424 1104 2 0.18710457164486272 1104 3 0.20014216348613445 1104 4 0.21928485080066906 1104 5 0.29498862678067184 1104 6 0.1602798397048062 1104 7 0.19835272312222685 1104 8 0.33541961771518913 1104 9 0.37209504286743567 1104 10-11 0.3899172499152215 1104 12-13 0.41167962794209245 1104 14-15 0.5365799710145751 1104 16-17 0.386650653266031 1104 18-19 0.5214665373880081 1104 20-21 0.545427060724947 1104 22-23 0.45100797372752055 1104 24-25 0.5728556978137576 1104 26-27 0.4935106196661252 1104 28-29 0.5745976144651834 1104 30-31 0.4966853431001965 1104 32-33 0.5777393928845456 1104 34-35 0.43654138026177236 1104 36-37 0.5218751762582912 1104 38-39 0.7047207522242616 1104 40-41 0.5661585099837225 1104 42-43 0.5681119698784016 1104 44-45 0.6602293163119128 1104 46-47 0.8938726125580629 1104 48-49 0.7758498787960093 1104 50-51 0.6817868467266024 1104 52-53 0.6956677707518892 1104 54-55 0.8867205032177381 1104 56-57 1.0093790673560292 1104 58-59 0.8392106240760384 1104 60-61 0.868504460908774 1104 62-63 1.011338929385289 1104 64-65 0.8980020654023875 1104 66-67 0.9930317456357827 1104 68-69 1.023702811668798 1104 70-71 1.0618057050351553 1104 72-73 0.8749189803398743 1104 74-75 0.8305739143536073 1104 76-77 0.6790731895136197 1104 78-79 0.5771656330521857 1104 80-81 0.5780898597433648 1104 82-83 0.641283881190823 1104 84-85 0.7333893581645654 1104 86-87 0.7114308050891083 1104 88-89 0.9786533734902214 1104 90-91 1.0095634028097216 1104 92-93 0.915585461664044 1104 94-95 0.9540066862660588 1104 96-97 0.955786997267186 1104 98-99 1.1170779888793128 1104 100-101 1.419163461132257 1105 1 0.03341029814063745 1105 2 0.1722711966117707 1105 3 0.05783989931044431 1105 4 0.241274391042559 1105 5 0.0469896081420913 1105 6 0.16611213864109686 1105 7 0.10912778002418833 1105 8 0.280802239359069 1105 9 0.15570606423634104 1105 10-11 0.23186826443401998 1105 12-13 0.1137811745579782 1105 14-15 0.325715401319691 1105 16-17 0.2496280649495688 1105 18-19 0.2780852614724125 1105 20-21 0.23989876520884224 1105 22-23 0.060998184404439826 1105 24-25 0.3440284490021952 1105 26-27 -0.01811693424194516 1105 28-29 0.20311588017607107 1105 30-31 0.3786353373917777 1105 32-33 0.2578495774242171 1105 34-35 0.34483995704479753 1105 36-37 0.35314416888108013 1105 38-39 0.33861464373524086 1105 40-41 0.22411944013945373 1105 42-43 0.4623550948829589 1105 44-45 0.6300251251213353 1105 46-47 0.5391347502255783 1105 48-49 0.6415563982908452 1105 50-51 0.9175546770190977 1105 52-53 0.8292604523219751 1105 54-55 0.9329908439979206 1105 56-57 0.9005621179811669 1105 58-59 0.8002118313449174 1105 60-61 0.8000514654487816 1105 62-63 0.7393032360072951 1105 64-65 0.743004652407123 1105 66-67 0.9533461213660317 1105 68-69 0.6287188073741277 1105 70-71 0.5115868420055136 1105 72-73 0.6729807915346804 1105 74-75 0.5433149733980436 1105 76-77 0.38562790648558476 1105 78-79 0.5915283675526268 1105 80-81 0.6255292108788524 1105 82-83 0.5717055993992091 1105 84-85 0.6535274289148951 1105 86-87 0.6545877524721035 1105 88-89 0.7186017062689096 1105 90-91 0.7317075560575645 1105 92-93 0.5714743589198754 1105 94-95 0.5606343980664441 1105 96-97 0.7514354852661533 1105 98-99 0.8327522011788808 1105 100-101 1.0792960177973754 1106 1 0.10817305720581416 1106 2 0.13671511732784225 1106 3 0.05720982201775371 1106 4 0.06546839713170272 1106 5 0.12651642490503434 1106 6 0.16365916597491292 1106 7 0.08433832684470133 1106 8 -0.06935960873939706 1106 9 0.05530493105916179 1106 10-11 0.1853339300313266 1106 12-13 0.13058163244620147 1106 14-15 0.18885636067249578 1106 16-17 0.06783753919576441 1106 18-19 0.20884409578132335 1106 20-21 0.2647567573304741 1106 22-23 0.27984422910625995 1106 24-25 0.21308780613096445 1106 26-27 0.24045283840115417 1106 28-29 0.1876393060650372 1106 30-31 0.010922710354257958 1106 32-33 0.22171272078843884 1106 34-35 0.14495034081477343 1106 36-37 0.1183225384719151 1106 38-39 0.3781495889762283 1106 40-41 0.28789793189795176 1106 42-43 -0.05258038231642104 1106 44-45 -0.15650492860358156 1106 46-47 -0.12849587056889078 1106 48-49 0.06513547780082973 1106 50-51 -0.08670074958170915 1106 52-53 -0.09754837453182574 1106 54-55 -0.0013079665508186622 1106 56-57 0.061697170960563597 1106 58-59 0.07785226403005652 1106 60-61 0.1905274383410287 1106 62-63 0.3431168870406651 1106 64-65 -0.024341712143097993 1106 66-67 0.004182777435211449 1106 68-69 -0.19403467849242162 1106 70-71 -0.07930661721501764 1106 72-73 0.05485693084428789 1106 74-75 0.06959544917854643 1106 76-77 -0.19274457019914948 1106 78-79 0.2445974692355577 1106 80-81 0.02916225578749021 1106 82-83 -0.13147629092886604 1106 84-85 0.05720255913888295 1106 86-87 0.09415564450282687 1106 88-89 0.2115301708523134 1106 90-91 0.4455238043561174 1106 92-93 0.09981461027742711 1106 94-95 0.16597995457860648 1106 96-97 0.33199279103640933 1106 98-99 0.2571561528697508 1106 100-101 2.2011512748321138E-4 1107 1 0.03669112107255401 1107 2 -0.13557974685983964 1107 3 -0.09500038618695683 1107 4 -0.04775177328530589 1107 5 0.04521641265345977 1107 6 -0.013616323907932326 1107 7 -0.1333229195008485 1107 8 -0.013843156795601885 1107 9 -0.27053652394752703 1107 10-11 -0.22197929950794304 1107 12-13 -0.06358361487946951 1107 14-15 -0.07788986115789243 1107 16-17 -0.08506992777903122 1107 18-19 -0.010622456216999865 1107 20-21 0.06270906266855292 1107 22-23 0.07040234841738169 1107 24-25 -0.02757997679033508 1107 26-27 0.08972543114678189 1107 28-29 0.166737758282693 1107 30-31 0.1412938850400991 1107 32-33 0.17421444441692557 1107 34-35 0.10116717447922952 1107 36-37 0.026373264798152718 1107 38-39 0.1150956556199958 1107 40-41 -0.3112016974318479 1107 42-43 -0.06267351831130696 1107 44-45 -0.20053012360781963 1107 46-47 0.15379904166418612 1107 48-49 0.16221900231478514 1107 50-51 0.17970735279341454 1107 52-53 0.3438044155842803 1107 54-55 0.4593038194646013 1107 56-57 0.4275478266600672 1107 58-59 0.5192567029439417 1107 60-61 0.41767284532154036 1107 62-63 0.3199836307401185 1107 64-65 0.2363766145319488 1107 66-67 0.30129548952559304 1107 68-69 0.3859302723931748 1107 70-71 0.11513429055464286 1107 72-73 0.2925933442076314 1107 74-75 0.235042763801399 1107 76-77 0.19720385307115862 1107 78-79 0.22351274839130397 1107 80-81 0.32354779124138844 1107 82-83 0.24915793228522887 1107 84-85 0.39474546968076396 1107 86-87 0.3913434275202228 1107 88-89 0.6606861334057363 1107 90-91 0.6841256353660548 1107 92-93 0.5651870942002368 1107 94-95 0.798715563181613 1107 96-97 0.9001265638249443 1107 98-99 0.8641003937885685 1107 100-101 0.8861663571369363 1108 1 0.22659302928432012 1108 2 0.15267416580721616 1108 3 0.19725526287620454 1108 4 0.11097769060847895 1108 5 -4.44238640994854E-4 1108 6 -0.009454080309069468 1108 7 0.17786163581516234 1108 8 0.1536290857940088 1108 9 0.2927197294495727 1108 10-11 0.1414533902158226 1108 12-13 0.2960917471433149 1108 14-15 0.16786733765095363 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-1.8562334010973203 2316 66-67 -1.7686829600616036 2316 68-69 -1.8896589129866932 2316 70-71 -1.9380075017168181 2316 72-73 -1.9703162430470194 2316 74-75 -1.8305765630955655 2316 76-77 -1.8868563791066144 2316 78-79 -1.8752368191019926 2316 80-81 -1.6904963031009252 2316 82-83 -1.3996402666705805 2316 84-85 -1.6871329307754905 2316 86-87 -1.7239476483566634 2316 88-89 -1.8485365896110686 2316 90-91 -1.7153239345792493 2316 92-93 -1.5066081105831728 2316 94-95 -1.7420009131215615 2316 96-97 -1.7657099018710305 2316 98-99 -1.9922960284614852 2316 100-101 -2.106036262150912 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2839.0 3 643.0 4 584.0 5 695.0 6 809.0 7 980.0 8 1225.0 9 1516.0 10 2092.0 11 2931.0 12 4315.0 13 6601.0 14 5981.0 15 6667.0 16 9021.0 17 12809.0 18 10433.0 19 6455.0 20 6208.0 21 6379.0 22 6991.0 23 7700.0 24 8855.0 25 9957.0 26 11725.0 27 13611.0 28 16156.0 29 19071.0 30 22882.0 31 27589.0 32 34281.0 33 43566.0 34 59038.0 35 85525.0 36 123922.0 37 182469.0 38 167670.0 39 26525.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.38036809815951 19.40496041109538 14.882596612657961 19.33207487808715 2 14.506526399040892 23.623503614967042 38.52013888670941 23.349831099282657 3 22.401073877184192 26.705894292568754 31.73168973486826 19.161342095378792 4 11.74534915353548 17.16512859145793 40.790591583485046 30.298930671521546 5 11.397588192944564 40.23044435514927 34.56267684631741 13.809290605588762 6 26.767206668270287 38.31149272618886 17.684512168338266 17.236788437202588 7 24.557911778978898 36.17990595558828 22.118739436737563 17.14344282869526 8 24.036931945944986 30.958325009909665 20.836658344986127 24.16808469915922 9 24.454406064548788 19.9877838835118 23.934070623377547 31.623739428561866 10-11 24.220151725991666 25.935972196293083 27.559179882683498 22.28469619503175 12-13 28.83186887488489 24.942229585041346 26.769547421941304 19.456354118132456 14-15 23.707622099964077 26.298679057723312 29.89384248779118 20.099856354521435 16-17 19.266583730366033 29.613384015466103 30.055989846839765 21.064042407328095 18-19 18.883030464041635 30.485431104993527 31.312612569267362 19.31892586169748 20-21 21.78608765959539 26.74131753500469 29.63211671349997 21.840478091899957 22-23 20.22315049032223 26.93581559615944 30.44616498343973 22.39486893007861 24-25 22.434256677123663 26.98710373663647 30.321886088325257 20.256753497914616 26-27 21.92016353748144 25.07002808446577 33.50096483138597 19.50884354666682 28-29 19.275427665165793 29.00685017491589 31.87753167735132 19.840190482566992 30-31 20.043600561116627 27.4460720267043 30.982811880525258 21.52751553165382 32-33 20.425331396157656 27.64172919078984 31.275816369971267 20.65712304308124 34-35 22.318063510772838 26.322878936914982 30.583671175186293 20.775386377125887 36-37 21.638880469890424 25.749843861867262 32.65768900855831 19.953586659684007 38-39 21.256670591213148 26.936427598419115 32.105485927822656 19.70141588254508 40-41 20.240355434958385 27.940466114598582 31.669445908738652 20.14973254170438 42-43 19.46568293279861 28.618966638270877 29.475199549588822 22.440150879341694 44-45 20.75814398117509 26.912745685081884 29.657590369039738 22.671519964703286 46-47 22.24831404021093 28.143448391772935 29.0852223786214 20.523015189394737 48-49 21.5779492648179 27.456621892089238 29.690798010096607 21.274630832996255 50-51 19.614223917171607 28.448727343403224 31.524167474306548 20.412881265118624 52-53 20.704896030593297 29.696562193850344 28.623012735825515 20.97552903973084 54-55 19.138003300624725 27.5090052849914 30.148979821228416 23.204011593155457 56-57 21.68342537142569 27.74118776400999 28.46387408600818 22.11151277855614 58-59 23.045582296950418 26.999964822397736 28.51632949547443 21.43812338517742 60-61 21.062100730782067 27.436814957414786 31.075137664562426 20.425946647240725 62-63 20.97262202810957 27.570981629149248 30.30552259947092 21.150873743270264 64-65 20.297390934330764 29.45488835363307 29.511466703089745 20.73625400894642 66-67 20.797843388204836 27.558743608657547 28.423584447680835 23.219828555456782 68-69 19.743206171206744 28.346051647758653 29.47885123749383 22.431890943540772 70-71 22.028698268845684 28.14456428474223 27.943637520551118 21.883099925860964 72-73 21.185027962898264 26.481455699361412 30.3116241071968 22.021892230543525 74-75 19.84035709238284 30.248458920033016 28.530351118777038 21.38083286880711 76-77 21.18187376174699 27.168400308420853 28.638651772264396 23.011074157567762 78-79 20.83947526100676 27.51944192524029 29.839974667338144 21.8011081464148 80-81 21.784913224705136 28.07442188753006 28.637974215551022 21.502690672213788 82-83 20.762199781616385 27.51407702460466 29.86046663084713 21.863256562931824 84-85 20.55153179934558 27.922498778867315 29.103924956800793 22.422044464986318 86-87 20.824575390785434 28.44107047005598 28.775946881962366 21.958407257196217 88-89 20.99040511559086 27.682819920087276 27.906264592318525 23.42051037200334 90-91 20.945300996079457 26.453445459908604 29.12958488896874 23.471668655043203 92-93 21.825687861320265 28.31048692995194 28.892329450530514 20.971495758197282 94-95 21.03892951453434 27.72389435954449 28.868756455939888 22.368419669981286 96-97 19.479058038587322 27.607139573674466 29.88970172973115 23.024100658007065 98-99 21.183388182089463 28.30432233416132 27.494410050141433 23.01787943360779 100-101 21.244887336246375 26.43274079746787 28.93340917960492 23.388962686680838 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2848.0 1 1748.0 2 729.5 3 798.0 4 704.5 5 701.5 6 1051.0 7 1965.0 8 3009.0 9 3369.0 10 2860.0 11 2307.5 12 2275.0 13 2373.5 14 2491.0 15 2644.5 16 2793.5 17 2827.5 18 2759.0 19 2857.5 20 2998.5 21 3259.5 22 3737.0 23 4225.5 24 4711.5 25 5422.0 26 6780.5 27 8154.5 28 8909.0 29 9840.0 30 11466.5 31 13014.0 32 15067.0 33 17883.5 34 20804.0 35 22628.0 36 24299.0 37 26483.5 38 26626.0 39 25228.5 40 24023.0 41 24076.0 42 24463.5 43 25212.5 44 26520.5 45 27094.5 46 30867.0 47 49026.5 48 93481.5 49 91717.5 50 46030.0 51 27155.0 52 20920.5 53 18583.5 54 16874.5 55 15497.5 56 14026.0 57 12744.0 58 11863.0 59 10698.0 60 9438.5 61 8184.5 62 6799.5 63 5091.0 64 3569.0 65 2620.5 66 1778.5 67 1308.5 68 963.0 69 633.0 70 475.5 71 375.0 72 321.5 73 287.5 74 228.5 75 185.0 76 133.5 77 78.5 78 47.0 79 25.0 80 9.0 81 11.5 82 14.0 83 12.0 84 9.5 85 5.5 86 3.0 87 2.0 88 1.0 89 1.0 90 0.5 91 1.5 92 2.5 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33134041307801654 2 0.08706831674889204 3 0.09731164813111465 4 0.10243331382222595 5 0.1137218831005937 6 0.057488084287983944 7 0.06020570281796137 8 0.06093736934526298 9 0.06334141650639685 10-11 0.06151225018814282 12-13 0.05900367923739443 14-15 0.05712225102433313 16-17 0.05900367923739443 18-19 0.06710427293251944 20-21 0.06997867714691863 22-23 0.0855004599046743 24-25 0.0941236725478719 26-27 0.08921105443598963 28-29 0.08696379295927753 30-31 0.080378794213563 32-33 0.0720168910444017 34-35 0.3259051760180617 36-37 0.505738356049837 38-39 0.4800777656994732 40-41 0.4622041976753909 42-43 0.491366334977841 44-45 0.5006689522535329 46-47 0.4954427627728071 48-49 0.5137344259553475 50-51 0.5092921648967305 52-53 0.509814783844803 54-55 0.4995191905677732 56-57 0.4785099088552554 58-59 0.46089765030520946 60-61 0.4224851576218747 62-63 0.4907914541349611 64-65 0.5166088301697467 66-67 0.5270089472363909 68-69 0.5372000167238064 70-71 0.5361547788276612 72-73 0.5239254954427628 74-75 0.5261204950246676 76-77 0.4989965716197006 78-79 0.47971193243582244 80-81 0.48441550296847563 82-83 0.4939271678233966 84-85 0.4949201438247345 86-87 0.49408395350781836 88-89 0.5023935947821724 90-91 0.5161907350112886 92-93 0.5228279956518103 94-95 0.5306672798728991 96-97 0.5207897817543273 98-99 0.5238732335479555 100-101 0.5011393093067983 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 956720.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.7489340994659 #Duplication Level Percentage of deduplicated Percentage of total 1 74.70364876755468 30.44094060618733 2 13.151339065763098 10.718060978210236 3 4.75417027831867 5.8118211410654075 4 2.3283522173480264 3.7951148426024006 5 1.265695247166816 2.578786610840389 6 0.7946139352825361 1.9427802530007192 7 0.525526045260524 1.499023833011182 8 0.3767814377432633 1.22827535812018 9 0.2881200588578023 1.056652676201774 >10 1.5945008429632073 12.243272302711231 >50 0.12932313900847078 3.736300731162602 >100 0.07173152386247914 5.129363959344711 >500 0.004884942484722138 1.434566599074798 >1k 0.00848437378925424 8.648048965692563 >5k 0.0023139201243420654 6.1903335017550996 >10k+ 5.142044720760145E-4 3.546657641019436 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21231 2.2191445773057947 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12622 1.3192992725144241 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8976 0.9382055355798979 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7488 0.7826741366334978 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7251 0.7579019984948574 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7120 0.7442093820553558 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5982 0.6252613094740362 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5862 0.6127184547202944 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5738 0.5997575048080943 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5407 0.5651601304456895 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5263 0.5501087047411991 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4982 0.52073751985952 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4955 0.5179153775399281 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4881 0.5101806171084539 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4452 0.46533991136382646 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4309 0.4503930094489506 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3826 0.3999080190651393 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3585 0.3747177857680408 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3054 0.3192156534827327 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3008 0.3144075591604649 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2830 0.295802324609081 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2799 0.29256208713103105 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2753 0.2877539928087633 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2636 0.27552470942386487 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2492 0.2604732837193745 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2427 0.253679237394431 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2266 0.23685090726649388 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2261 0.2363282883184213 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2084 0.21782757755665189 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2068 0.21615519692281962 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1997 0.20873400786018897 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1957 0.20455305627560832 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1946 0.20340329458984865 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1776 0.1856342503553809 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1679 0.17549544276277282 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1663 0.17382306212894055 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1524 0.15929425537252279 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1282 0.13399949828580984 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1227 0.12825068985701144 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1223 0.1278325946985534 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1206 0.1260556902751066 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1175 0.1228154527970566 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1118 0.11685759678902918 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1105 0.11549878752404048 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 981 0.10253783761184046 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.0452378961451626E-4 0.0 18-19 0.0 0.0 0.0 3.135713688435488E-4 0.0 20-21 0.0 0.0 0.0 7.839284221088721E-4 0.0 22-23 0.0 0.0 0.0 0.0015155949494104859 0.0 24-25 0.0 0.0 0.0 0.002926666109206455 0.0 26-27 0.0 0.0 0.0 0.0059055941132201695 0.0 28-29 0.0 0.0 0.0 0.017403210970816957 0.0 30-31 0.0 0.0 0.0 0.04698344343172506 0.0 32-33 0.0 0.0 0.0 0.09997700476628481 0.0 34-35 0.0 0.0 0.0 0.18202817961368006 0.0 36-37 0.0 0.0 0.0 0.2933982774479471 0.0 38-39 0.0 0.0 0.0 0.4405155113303788 0.0 40-41 0.0 0.0 0.0 0.6055063132368927 0.0 42-43 0.0 0.0 0.0 0.7704448532485993 0.0 44-45 0.0 0.0 0.0 0.9696149343590601 0.0 46-47 0.0 0.0 0.0 1.2140960782674135 0.0 48-49 0.0 0.0 0.0 1.4595179362822979 0.0 50-51 0.0 0.0 0.0 1.7232837193745296 0.0 52-53 0.0 0.0 0.0 1.9641065306463752 0.0 54-55 0.0 0.0 0.0 2.2333598126933687 0.0 56-57 0.0 0.0 0.0 2.5756229617861024 0.0 58-59 0.0 0.0 0.0 2.929592775315662 0.0 60-61 0.0 0.0 0.0 3.3492035287231374 0.0 62-63 0.0 0.0 0.0 3.761288569278368 0.0 64-65 0.0 0.0 0.0 4.1479220670624635 0.0 66-67 0.0 0.0 0.0 4.590214482816289 0.0 68-69 0.0 0.0 0.0 5.110377121832929 0.0 70-71 0.0 0.0 0.0 5.667018563425035 0.0 72-73 0.0 0.0 0.0 6.22256250522619 0.0 74-75 0.0 0.0 0.0 6.843224767957187 0.0 76-77 0.0 0.0 0.0 7.429760013379045 0.0 78-79 0.0 0.0 0.0 8.185205702817962 0.0 80-81 0.0 0.0 0.0 8.98063174178443 0.0 82-83 0.0 0.0 0.0 9.710155531398946 0.0 84-85 0.0 0.0 0.0 10.541171920729159 0.0 86-87 0.0 0.0 0.0 11.41697675390919 0.0 88-89 0.0 0.0 0.0 12.280290994230286 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 140 0.0 32.271446 94-95 GCGCATC 35 0.008343188 27.175953 94-95 CTTTGCG 705 0.0 26.874504 2 GTCCTAC 195 5.3842086E-10 26.776901 1 TTGCGTT 740 0.0 25.603413 4 TTTGCGT 740 0.0 25.603413 3 GTACATG 6300 0.0 24.035458 1 GAGTACT 5180 0.0 23.94996 12-13 GTACGTG 120 1.718316E-4 23.734072 1 TACATGG 6500 0.0 22.954445 2 GTCTTCG 145 2.3459374E-5 22.915655 1 AGTACTT 5235 0.0 22.884272 12-13 GTACACG 105 0.0022045262 22.603878 1 GCTTTGC 845 0.0 22.470129 1 GTATAGG 280 6.730261E-11 22.038782 1 GTCGGGA 215 4.553476E-8 22.030844 2 ACATGGG 6490 0.0 21.822077 3 GTACTTT 5755 0.0 21.762743 14-15 ATGGGAT 1585 0.0 21.516558 5 CATGGGA 4715 0.0 21.196802 4 >>END_MODULE