##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938909_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 956720 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16181850489171 33.0 31.0 34.0 30.0 34.0 2 32.38024291328706 34.0 31.0 34.0 30.0 34.0 3 32.45902144828163 34.0 31.0 34.0 30.0 34.0 4 35.98325110795217 37.0 35.0 37.0 35.0 37.0 5 35.92873672547872 37.0 35.0 37.0 35.0 37.0 6 36.009723848147836 37.0 35.0 37.0 35.0 37.0 7 35.95794171753491 37.0 35.0 37.0 35.0 37.0 8 35.95142884020403 37.0 35.0 37.0 35.0 37.0 9 37.58855359979931 39.0 37.0 39.0 35.0 39.0 10-11 37.501993268667945 39.0 37.0 39.0 34.5 39.0 12-13 37.438476252195 39.0 37.0 39.0 34.0 39.0 14-15 38.608735575717034 40.0 38.0 41.0 34.0 41.0 16-17 38.74828476461242 40.0 38.0 41.0 34.5 41.0 18-19 38.87176394347354 40.0 38.0 41.0 35.0 41.0 20-21 38.85355694456058 40.0 38.0 41.0 34.5 41.0 22-23 38.78715768458901 40.0 38.0 41.0 34.5 41.0 24-25 38.674600719123674 40.0 38.0 41.0 34.0 41.0 26-27 38.45084141650639 40.0 38.0 41.0 34.0 41.0 28-29 38.30809954845723 40.0 38.0 41.0 34.0 41.0 30-31 38.13270758424618 40.0 37.5 41.0 34.0 41.0 32-33 37.96805909775065 40.0 37.0 41.0 33.0 41.0 34-35 37.80783928422109 40.0 37.0 41.0 33.0 41.0 36-37 37.61849600719124 40.0 36.5 41.0 33.0 41.0 38-39 37.42174669704825 40.0 36.0 41.0 32.5 41.0 40-41 37.29356603813028 40.0 36.0 41.0 32.0 41.0 42-43 37.079251505142565 40.0 36.0 41.0 31.5 41.0 44-45 36.7729518563425 40.0 36.0 41.0 31.0 41.0 46-47 36.34382943808011 40.0 35.5 41.0 30.0 41.0 48-49 35.907385128355216 39.0 35.0 41.0 28.5 41.0 50-51 35.61729084789698 39.0 35.0 41.0 28.0 41.0 52-53 35.379490342001844 39.0 35.0 40.0 27.0 41.0 54-55 35.13653210970817 38.5 35.0 40.0 26.5 41.0 56-57 35.00486453716866 38.5 35.0 40.0 26.0 41.0 58-59 34.75750742118906 38.0 34.0 40.0 26.0 41.0 60-61 34.32517298687181 38.0 34.0 40.0 24.5 41.0 62-63 33.94025786018898 37.5 34.0 40.0 23.0 41.0 64-65 33.52153817208797 37.0 33.0 39.5 22.0 41.0 66-67 33.032248202190814 36.0 33.0 39.0 21.5 40.0 68-69 32.524500376285644 36.0 32.0 39.0 20.5 40.0 70-71 31.91861986788193 35.0 31.5 37.5 18.0 40.0 72-73 31.37023319257463 35.0 31.0 37.0 15.0 39.0 74-75 30.87537733088051 35.0 31.0 37.0 10.0 39.0 76-77 28.947414604063884 32.5 28.0 35.0 7.0 37.0 78-79 29.888377999832763 34.0 29.5 35.0 6.5 37.0 80-81 29.850387260640524 34.0 30.0 35.0 2.0 37.0 82-83 29.438900618780835 34.0 29.5 35.0 2.0 36.5 84-85 28.997273496947905 34.0 29.0 35.0 2.0 36.0 86-87 28.399285579897985 34.0 29.0 35.0 2.0 36.0 88-89 27.682091939125343 33.0 26.5 35.0 2.0 35.0 90-91 27.077881198260723 33.0 25.5 35.0 2.0 35.0 92-93 26.734916694539677 33.0 25.0 34.5 2.0 35.0 94-95 26.216195961200768 32.0 25.0 34.0 2.0 35.0 96-97 25.360901308637846 32.0 21.5 34.0 2.0 35.0 98-99 24.26356509741617 32.0 10.0 34.0 2.0 35.0 100-101 18.91531691613011 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37890924722217534 1101 2 0.24100057888234971 1101 3 0.16272600241348556 1101 4 0.12809024874940889 1101 5 0.2642595168311601 1101 6 0.2409172023938666 1101 7 0.26024393384348343 1101 8 0.2503343480533573 1101 9 0.3069270491814322 1101 10-11 0.31206102371663746 1101 12-13 0.327367978598339 1101 14-15 0.4683598374043143 1101 16-17 0.37851025209783273 1101 18-19 0.35526507148168207 1101 20-21 0.4015762096298232 1101 22-23 0.3969962309207844 1101 24-25 0.41974092726475476 1101 26-27 0.5366750093984862 1101 28-29 0.43889702353216364 1101 30-31 0.4569600737611026 1101 32-33 0.5577766713538708 1101 34-35 0.5621217340239255 1101 36-37 0.7203884588339093 1101 38-39 0.6725569694709819 1101 40-41 0.6549400120100515 1101 42-43 0.795556614456217 1101 44-45 0.6731788149501554 1101 46-47 0.7589149097259309 1101 48-49 0.5893600171834734 1101 50-51 0.4767852190819326 1101 52-53 0.5805124814196247 1101 54-55 0.5458196482313511 1101 56-57 0.5952559598390579 1101 58-59 0.6874777731285917 1101 60-61 0.7029122204701395 1101 62-63 0.855641832335067 1101 64-65 0.7007508808666003 1101 66-67 0.6685723113837199 1101 68-69 0.751981223876335 1101 70-71 1.096803608837316 1101 72-73 0.8414591127120516 1101 74-75 0.9554456210465254 1101 76-77 0.6367147456763504 1101 78-79 0.9214969486553386 1101 80-81 1.2175653279425234 1101 82-83 0.974602243780808 1101 84-85 0.8005832636780958 1101 86-87 0.7191187716624441 1101 88-89 0.769295411133271 1101 90-91 0.9149563029737635 1101 92-93 0.8095619910555989 1101 94-95 0.4618196855623431 1101 96-97 0.7825878779886857 1101 98-99 1.0864826021083758 1101 100-101 1.2896111817047888 1102 1 0.10168956039682087 1102 2 0.09151433834454537 1102 3 0.20367782380009203 1102 4 0.06008817184945059 1102 5 0.04318171506579915 1102 6 0.05919528836659538 1102 7 0.12415369800602605 1102 8 0.11981211702556749 1102 9 0.150893108263773 1102 10-11 0.20988779386393475 1102 12-13 0.07311648241054769 1102 14-15 0.16798886481392827 1102 16-17 0.2215212104778459 1102 18-19 0.28512119150463633 1102 20-21 0.18364791733348085 1102 22-23 0.18948728112165014 1102 24-25 0.21552085785257447 1102 26-27 0.31880068917330817 1102 28-29 0.2861430815214021 1102 30-31 0.24925163225589841 1102 32-33 0.27815146981259176 1102 34-35 0.166449747578433 1102 36-37 0.3141440951578005 1102 38-39 0.16922778082835066 1102 40-41 0.12935588389812125 1102 42-43 0.23827311761255743 1102 44-45 0.30858790958574644 1102 46-47 0.6222747053152489 1102 48-49 0.5311405489791667 1102 50-51 0.46869419074317875 1102 52-53 0.2862082429040598 1102 54-55 0.25452554826687646 1102 56-57 0.010510243445210676 1102 58-59 -0.1468839900501493 1102 60-61 -0.020724130744575575 1102 62-63 0.08854088406627625 1102 64-65 0.23068010230239366 1102 66-67 -0.023187041497983785 1102 68-69 -0.012501737345758102 1102 70-71 0.0992856409199554 1102 72-73 0.2600904083150368 1102 74-75 0.46364732629070105 1102 76-77 0.2205388377158073 1102 78-79 0.4470605810440489 1102 80-81 0.2851936994889961 1102 82-83 0.2344661521785909 1102 84-85 0.44050278380489516 1102 86-87 0.7172106198301265 1102 88-89 0.5449396600333323 1102 90-91 0.5841532805377803 1102 92-93 0.694445711985832 1102 94-95 0.6075934674331904 1102 96-97 0.6792709867799864 1102 98-99 0.7660469996707491 1102 100-101 1.2460482051792248 1103 1 -0.07060009543140922 1103 2 -0.09104001797206251 1103 3 -0.03178266810674302 1103 4 -0.09344490382486015 1103 5 -0.06410451059187494 1103 6 -0.04614423059443595 1103 7 0.11433050461912586 1103 8 0.06875080187779758 1103 9 0.16985889485961536 1103 10-11 -0.056372233441585706 1103 12-13 0.018741147034951666 1103 14-15 -0.044346843601196895 1103 16-17 -0.16978925052348615 1103 18-19 -0.03176611550581754 1103 20-21 -0.027802739153614198 1103 22-23 0.002706003739717744 1103 24-25 -0.019763710455372063 1103 26-27 -0.25066535893058983 1103 28-29 -0.1551982849770468 1103 30-31 -0.1392121268034927 1103 32-33 0.0019391931982042365 1103 34-35 -0.11025022457265976 1103 36-37 -0.2742075890219837 1103 38-39 -0.07501326846421819 1103 40-41 -0.24141702522612718 1103 42-43 -0.1687026945906993 1103 44-45 -0.07173649624758838 1103 46-47 0.24368100731968667 1103 48-49 0.038778621834630655 1103 50-51 0.02635841440388731 1103 52-53 0.04869032790436023 1103 54-55 0.26758668681180353 1103 56-57 0.08916643167666649 1103 58-59 0.004267664242995295 1103 60-61 0.01865694422333064 1103 62-63 -0.019821939677299838 1103 64-65 -0.10314749257069167 1103 66-67 0.0306707117063425 1103 68-69 0.10328180191101666 1103 70-71 0.017797694063823144 1103 72-73 -0.13282170460210452 1103 74-75 -0.20228055182617055 1103 76-77 -0.43697459428869934 1103 78-79 -0.1800153513849878 1103 80-81 -0.23571132214485502 1103 82-83 0.10373250322428618 1103 84-85 0.2558359858181696 1103 86-87 0.11303260416681127 1103 88-89 -0.1712140659469803 1103 90-91 0.05279404998277215 1103 92-93 0.11583299522282786 1103 94-95 0.4052663853875913 1103 96-97 -0.018168272031477528 1103 98-99 0.19675279971558268 1103 100-101 0.5100171502489452 1104 1 0.09598781697659575 1104 2 0.11757418757007798 1104 3 0.1711806496699282 1104 4 0.09321434052262845 1104 5 -0.03342271447694145 1104 6 0.17413811960525294 1104 7 0.19415236895898147 1104 8 0.1875566715513699 1104 9 0.12425953179816673 1104 10-11 0.2575420258260692 1104 12-13 0.22193619652068008 1104 14-15 0.3107535195314526 1104 16-17 0.2646895796844859 1104 18-19 0.374345646496657 1104 20-21 0.3866552481464538 1104 22-23 0.3488406799949857 1104 24-25 0.37222471590508377 1104 26-27 0.3526591496104672 1104 28-29 0.26287225726220953 1104 30-31 0.15175616220090404 1104 32-33 0.39374961335757064 1104 34-35 0.3998915978224602 1104 36-37 0.40454429622550947 1104 38-39 0.44324175292889123 1104 40-41 0.4662475981451166 1104 42-43 0.24711720197151266 1104 44-45 0.3041737627352532 1104 46-47 0.3804486736260273 1104 48-49 0.25546395218177764 1104 50-51 0.4923657029487032 1104 52-53 0.48681407781536024 1104 54-55 0.7073739855838852 1104 56-57 0.3684193234683235 1104 58-59 0.5583103468935349 1104 60-61 0.514767346905721 1104 62-63 0.40298486210146933 1104 64-65 0.6290198462042369 1104 66-67 0.4799046377463867 1104 68-69 0.5076083235403885 1104 70-71 0.5752529174130565 1104 72-73 0.7000602481582874 1104 74-75 0.5217852392657498 1104 76-77 0.22474112657168632 1104 78-79 0.5027581794165599 1104 80-81 0.5900665649539043 1104 82-83 0.3909568363483231 1104 84-85 0.5651272622197254 1104 86-87 0.7134682338880225 1104 88-89 0.6906071640435023 1104 90-91 0.8272919106718817 1104 92-93 0.7806719157932243 1104 94-95 1.4171805022502753 1104 96-97 1.407876948016554 1104 98-99 1.398389162175043 1104 100-101 1.3389926760145379 1105 1 -0.11570811644629231 1105 2 -0.0072287506843125016 1105 3 7.954167301917892E-4 1105 4 0.03613141305349643 1105 5 0.11910489845856631 1105 6 0.01941944726094391 1105 7 0.10507317260653792 1105 8 0.06783422193723254 1105 9 0.10671769640940454 1105 10-11 0.1743005939128821 1105 12-13 0.15965496845860372 1105 14-15 0.016524847745834848 1105 16-17 0.004691960214664448 1105 18-19 0.11351180507397629 1105 20-21 0.08864930653745517 1105 22-23 0.13923378325898028 1105 24-25 0.030015608190950616 1105 26-27 0.02733358781749473 1105 28-29 0.11160171000746999 1105 30-31 0.07567696827756976 1105 32-33 0.0803224515082448 1105 34-35 0.17702235451527315 1105 36-37 0.1790248912608945 1105 38-39 0.15407717327465065 1105 40-41 0.23669100832746892 1105 42-43 0.26969398014254153 1105 44-45 0.13341566103803615 1105 46-47 0.2671591209956645 1105 48-49 0.2893209211838723 1105 50-51 0.487438248391328 1105 52-53 0.5617224395490652 1105 54-55 0.48993380855016966 1105 56-57 0.3837415573772702 1105 58-59 0.4752091962229841 1105 60-61 0.4651442236987151 1105 62-63 0.4814287969721036 1105 64-65 0.36830624584667504 1105 66-67 0.2771053285859608 1105 68-69 0.306048468995634 1105 70-71 0.23593870443333387 1105 72-73 -0.053373909534833786 1105 74-75 0.23584591363594498 1105 76-77 0.20863246751461872 1105 78-79 0.23882782692805193 1105 80-81 0.11263544850954332 1105 82-83 0.13181608628698882 1105 84-85 0.21147339130761722 1105 86-87 0.225105504245807 1105 88-89 0.4487914510868585 1105 90-91 0.60166744659481 1105 92-93 0.8549663193611003 1105 94-95 0.6059729418807613 1105 96-97 0.5826849874070383 1105 98-99 0.2706020593045615 1105 100-101 0.34630794808727217 1106 1 0.015566804072584262 1106 2 0.0514141301637423 1106 3 0.02935571416514904 1106 4 0.05683257845600309 1106 5 0.06836924714622228 1106 6 0.15112965018430913 1106 7 0.06267522348407795 1106 8 0.1250726556901327 1106 9 0.06265076392386959 1106 10-11 0.11612305640884557 1106 12-13 0.12638953276736942 1106 14-15 0.03196655116701663 1106 16-17 -0.07552986547036511 1106 18-19 -0.10789302680120016 1106 20-21 0.02312006566035052 1106 22-23 -0.011443670074413603 1106 24-25 0.17845412274765948 1106 26-27 0.02679128846825307 1106 28-29 0.04122905055221793 1106 30-31 0.04422480845919097 1106 32-33 -0.1368077147648279 1106 34-35 -0.09436901005899045 1106 36-37 -0.14916241003629693 1106 38-39 -0.06075272990508296 1106 40-41 0.03550516438462381 1106 42-43 -0.04314420542724662 1106 44-45 0.04041312629961169 1106 46-47 0.28032340763754604 1106 48-49 0.3569604400164508 1106 50-51 0.17390144658670437 1106 52-53 0.10632205188318977 1106 54-55 0.05819138029962545 1106 56-57 0.04819774295668111 1106 58-59 0.05909124190598902 1106 60-61 0.28417736768820845 1106 62-63 0.34464430745495633 1106 64-65 0.2395251808717589 1106 66-67 0.10339157606684779 1106 68-69 -0.07745336592410723 1106 70-71 0.08920163905440504 1106 72-73 0.126908759491819 1106 74-75 0.22957159433585872 1106 76-77 -0.1007871880110649 1106 78-79 0.47006859821961555 1106 80-81 0.5208822464182639 1106 82-83 0.4171304756170393 1106 84-85 0.46928202096164995 1106 86-87 0.8123315288428685 1106 88-89 0.6059452332340278 1106 90-91 0.765542854822371 1106 92-93 0.6894567984603341 1106 94-95 0.5661253628685863 1106 96-97 0.5440133923495374 1106 98-99 0.3844019544520023 1106 100-101 0.7216175757645011 1107 1 -0.03777799609461141 1107 2 -0.05532711896843523 1107 3 -0.009484181768527833 1107 4 0.058846881569586174 1107 5 -0.05214301283700706 1107 6 -0.14205414986998477 1107 7 0.050806810207213005 1107 8 -0.04054221724181417 1107 9 -0.12482356181909893 1107 10-11 0.007777091140859227 1107 12-13 -0.03874668793395131 1107 14-15 -0.10010182703671688 1107 16-17 -0.05032645328653729 1107 18-19 -0.007305791981742971 1107 20-21 0.09897473315019312 1107 22-23 -0.03027265970381876 1107 24-25 -0.23731329430797388 1107 26-27 -0.08480134425114016 1107 28-29 -0.22908213946010392 1107 30-31 -0.13424340831201675 1107 32-33 -0.049777688796289965 1107 34-35 0.037764229425668816 1107 36-37 -0.03692717018168423 1107 38-39 0.11712032455677956 1107 40-41 -0.10626838326319898 1107 42-43 -0.04600590916493985 1107 44-45 0.1286154598643705 1107 46-47 -0.07102474609810372 1107 48-49 -0.1286103272444592 1107 50-51 -0.21314083095711567 1107 52-53 -0.06014802904928018 1107 54-55 -0.10722590595242565 1107 56-57 -0.03223024540672981 1107 58-59 -0.00505416267225911 1107 60-61 -0.2833264098808357 1107 62-63 0.025328017424129712 1107 64-65 0.15818493060330496 1107 66-67 0.028605318010242797 1107 68-69 -0.1261065334367757 1107 70-71 0.12466334327254813 1107 72-73 0.22793080512052555 1107 74-75 0.17607665231463798 1107 76-77 -1.651202814727526E-4 1107 78-79 0.2321333970415438 1107 80-81 0.15325090833003685 1107 82-83 -0.04382603609522562 1107 84-85 0.2821644322638477 1107 86-87 0.1539691080276846 1107 88-89 0.3196607516558352 1107 90-91 0.39501437951890495 1107 92-93 0.38675340429352545 1107 94-95 0.8471128398368251 1107 96-97 0.5913188865804848 1107 98-99 0.5440501901050503 1107 100-101 0.231653057287442 1108 1 0.02479184581795124 1108 2 -0.0024716107138686993 1108 3 0.06828155796904412 1108 4 0.0025088534005703877 1108 5 -0.04933900064332164 1108 6 0.0814217308828944 1108 7 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-0.854963342681458 2316 58-59 -0.9390529584646146 2316 60-61 -0.9394701846449678 2316 62-63 -0.9995907258044667 2316 64-65 -0.7408932057627666 2316 66-67 -0.8205921947470074 2316 68-69 -0.6308561608680101 2316 70-71 -0.6183277777394203 2316 72-73 -0.7854843949586652 2316 74-75 -0.7773683714289596 2316 76-77 -0.8480875896518256 2316 78-79 -0.9024109247924841 2316 80-81 -0.8971995797554833 2316 82-83 -1.1631470300841755 2316 84-85 -1.4380134925939565 2316 86-87 -1.56329365353103 2316 88-89 -1.399782933473837 2316 90-91 -1.734670882741355 2316 92-93 -1.6140203399839912 2316 94-95 -1.5725299118956997 2316 96-97 -1.4901372238651511 2316 98-99 -1.589078325005211 2316 100-101 -1.6197203842126768 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 3.0 5 42.0 6 75.0 7 197.0 8 354.0 9 679.0 10 1209.0 11 1784.0 12 2746.0 13 3695.0 14 4487.0 15 6164.0 16 8255.0 17 12077.0 18 11544.0 19 7082.0 20 6145.0 21 6422.0 22 6930.0 23 7746.0 24 8571.0 25 10096.0 26 11827.0 27 14087.0 28 16910.0 29 20332.0 30 24515.0 31 30097.0 32 37143.0 33 47407.0 34 63640.0 35 92574.0 36 141481.0 37 207389.0 38 135556.0 39 7453.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.36241405075938 20.07350286707357 15.297600557463205 19.26648252470385 2 14.718308386988879 24.055313989464004 37.82872731833765 23.397650305209467 3 22.39861192407392 26.64635421021825 31.85028012375617 19.10475374195167 4 11.90128773308805 17.593339744125764 40.2786604231123 30.226712099673886 5 11.317522368090978 40.59944393343925 34.51480056860942 13.568233129860358 6 26.91727987289907 38.54513337235555 17.280709089388747 17.256877665356633 7 24.580650556066562 36.222092148172926 21.731123003595616 17.4661342921649 8 24.325507985617527 31.21080357889456 20.574567271510997 23.88912116397692 9 24.34526298185467 19.914708587674554 23.731603813027846 32.008424617442934 10-11 24.319445605819883 26.168837277364325 27.32915586587507 22.182561250940715 12-13 28.82598879504975 24.91920311062798 26.73284764612426 19.52196044819801 14-15 23.452291048773795 26.510890157701507 29.78125612448881 20.25556266903589 16-17 19.287006303448788 29.725091029200946 29.924631427769565 21.063271239580704 18-19 18.965532559608953 30.50486848479876 31.183388565763348 19.34621038982894 20-21 21.721454553056276 26.78829751651476 29.613366502215904 21.87688142821306 22-23 20.212967221339575 26.99076009699808 30.428286227945478 22.367986453716863 24-25 22.41878501546952 27.18052303704323 30.20131281879756 20.19937912868969 26-27 21.879442261058617 25.232617693787105 33.427648632828834 19.460291412325446 28-29 19.272148591019317 29.101095409315157 31.787774897566685 19.83898110209884 30-31 20.144190567773226 27.395110377121835 30.89106530646375 21.56963374864119 32-33 20.348691362154025 27.767110544359895 31.24378083451794 20.640417258968142 34-35 22.26792258153542 26.31802327739003 30.66725834954973 20.74679579152482 36-37 21.61573148125883 25.789642615556886 32.72326464724191 19.87136125594238 38-39 21.177405874798705 26.93956742104815 32.19635139238866 19.686675311764493 40-41 20.222597370016516 27.974097380469548 31.65168398386051 20.151621265653418 42-43 19.46334541520434 28.625358340280194 29.562726861736184 22.34856938277928 44-45 20.788402712848715 26.90271258740272 29.673520562471356 22.63536413727721 46-47 22.257359985073855 28.24543056650889 29.04321631256268 20.453993135854574 48-49 21.63770747294581 27.474388282109896 29.65659758480011 21.231306660144185 50-51 19.585424693273016 28.52409392984737 31.430030291184195 20.46045108569541 52-53 20.732607241408143 29.763253616523123 28.53076135128355 20.973377790785182 54-55 19.124299690609583 27.503397023162474 30.026810352036122 23.34549293419182 56-57 21.653252780332803 27.84377874404214 28.318369010786853 22.184599464838197 58-59 22.970983476244456 27.053240076574646 28.507750752968363 21.468025694212532 60-61 20.8935793030857 27.771053294491605 30.87107493130052 20.464292471122178 62-63 20.798458877888752 27.74077289197146 30.15316625649168 21.307601973648108 64-65 20.167845441712696 29.62459503289125 29.25358532099735 20.953974204398705 66-67 20.65042137584112 27.67580845364866 28.19645913634285 23.477311034167375 68-69 19.603909064445002 28.404321315104692 29.24235875528314 22.74941086516717 70-71 21.80021322853081 28.282099255790616 27.75012542854754 22.16756208713103 72-73 20.94693327201271 26.518521615519692 30.085866293168323 22.44867881929927 74-75 19.53857973074672 30.27233673384062 28.14919725729576 22.0398862781169 76-77 20.698979292912384 27.20760864106599 28.425844939569455 23.667567126452173 78-79 20.451413187942315 27.34584912789953 29.663959225847186 22.53877845831097 80-81 21.24271664026566 27.928434539522335 28.47985065617525 22.348998164036757 82-83 20.20011079521699 27.36584371602977 29.820428129442263 22.61361735931098 84-85 19.77315178228926 27.684565687521623 29.08466331284317 23.45761921734595 86-87 19.955651086307757 28.25530076232215 28.64501461819289 23.144033533177204 88-89 20.112042406455036 27.406347424898676 27.828133751672578 24.65347641697371 90-91 20.114737781564827 26.018003988643752 29.016097813591507 24.851160416199917 92-93 20.687348440505062 28.0054770465758 28.850029266661092 22.457145246258047 94-95 19.93444206244101 27.252744036600397 28.844133703908494 23.9686801970501 96-97 17.972447529057614 27.2400493352287 29.966813696797388 24.820689438916297 98-99 19.631187808345178 27.75885316498035 27.57682498536667 25.033134041307804 100-101 19.38235556965472 25.933310372832885 29.152240138264716 25.53209391924768 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2040.0 1 1365.0 2 711.0 3 707.0 4 597.0 5 610.0 6 998.5 7 1860.5 8 2716.0 9 3036.5 10 2631.0 11 2119.0 12 2092.0 13 2103.5 14 2134.0 15 2232.5 16 2269.0 17 2355.5 18 2441.0 19 2580.0 20 2861.5 21 3156.0 22 3612.0 23 4067.0 24 4528.0 25 5320.0 26 6590.5 27 7941.0 28 8792.5 29 9790.0 30 11439.0 31 12963.5 32 15086.5 33 17825.5 34 20712.0 35 22775.0 36 24497.0 37 26597.5 38 26665.5 39 25354.5 40 24320.5 41 24110.5 42 24470.0 43 25391.0 44 26691.0 45 27276.0 46 30066.5 47 48515.5 48 92644.5 49 89711.0 50 47365.5 51 31668.0 52 24093.5 53 19673.0 54 17063.0 55 15431.0 56 14046.5 57 12618.5 58 11766.5 59 10892.0 60 9666.0 61 8219.5 62 6680.0 63 4968.0 64 3485.5 65 2570.0 66 1738.5 67 1221.0 68 874.0 69 557.5 70 396.0 71 305.5 72 250.5 73 245.5 74 197.5 75 155.0 76 121.5 77 53.0 78 17.5 79 10.5 80 6.0 81 4.5 82 2.5 83 2.0 84 2.0 85 1.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4005351618028263 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.839284221088719E-4 16-17 0.0022472614767121 18-19 5.748808428798394E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013222259386236307 36-37 0.025399280876327455 38-39 0.011340831173175015 40-41 0.006271427376870975 42-43 0.005487498954762104 44-45 0.0014110711597959695 46-47 9.929760013379044E-4 48-49 0.0030311898988209717 50-51 6.271427376870976E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.794046324943557E-4 60-61 0.0017769044234467765 62-63 0.0015155949494104859 64-65 6.794046324943557E-4 66-67 0.0033970231624717785 68-69 0.0021427376870975833 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09532569612843883 78-79 0.0037105945313153275 80-81 1.567856844217744E-4 82-83 0.0 84-85 1.0452378961451626E-4 86-87 0.09307843465172673 88-89 0.4414562254369095 90-91 0.39347980600384647 92-93 0.0 94-95 9.407141065306464E-4 96-97 0.0 98-99 0.0 100-101 4.703570532653232E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 956720.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.46285515428197 #Duplication Level Percentage of deduplicated Percentage of total 1 73.46103888410758 28.98982336966613 2 13.8045828955441 10.895365105442703 3 5.0483843187343 5.9767097740008035 4 2.4241397184596716 3.8265389833326355 5 1.2976609609862397 2.560470327138316 6 0.8166311609031748 1.9335958330317073 7 0.5498365099710919 1.518868298606758 8 0.3840385569270217 1.2124206356536424 9 0.28253170943933764 1.0034557133486637 >10 1.6931756407284948 12.493346539061523 >50 0.1420459916907728 3.895533515384339 >100 0.07870799667204205 5.383806604919528 >500 0.00477018161616233 1.312067553662282 >1k 0.008745332962964272 7.551761362183434 >5k 0.0031801210774415538 7.849188440345042 >10k+ 5.300201795735922E-4 3.5970479442223966 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22229 2.323459319341082 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12166 1.2716364244502047 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8476 0.8859436407726398 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7399 0.7733715193578059 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6958 0.7272765281378042 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6932 0.7245589096078267 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6092 0.636758926331633 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6078 0.6352955932770299 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5822 0.6085375031357138 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5795 0.6057153608161218 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5779 0.6040429801822894 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5358 0.5600384647545782 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5321 0.556171084538841 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5044 0.52721799481562 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4610 0.48185467012291994 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4171 0.4359687264821473 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3921 0.40983777907851826 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3870 0.40450706580817797 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3203 0.3347896981352956 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3016 0.31524374947738104 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2994 0.3129442261058617 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2845 0.29737018145329874 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2776 0.29015803996989714 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2698 0.28200518437996486 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2557 0.2672673300443181 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2529 0.26434066393511163 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2227 0.2327744794715277 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2136 0.22326281461660674 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2120 0.22159043398277448 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2054 0.21469186386821643 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2046 0.21385567355130028 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1961 0.20497115143406638 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1924 0.2011037712183293 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1820 0.1902332970984196 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1699 0.17758591855506314 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1657 0.17319591939125345 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1620 0.16932853917551635 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1371 0.1433021155615018 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1291 0.1349402123923405 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1262 0.13190902249351952 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1252 0.13086378459737436 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1192 0.1245923572205034 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1133 0.11842545363324694 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1085 0.11340831173175016 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1065 0.1113178359394598 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1053 0.11006355046408563 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1052 0.1099590266744711 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 997 0.10421021824567271 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.226189480725813E-5 0.0 14-15 0.0 0.0 0.0 1.0452378961451626E-4 0.0 16-17 0.0 0.0 0.0 2.0904757922903251E-4 0.0 18-19 0.0 0.0 0.0 4.1809515845806503E-4 0.0 20-21 0.0 0.0 0.0 8.361903169161302E-4 0.0 22-23 0.0 0.0 0.0 0.0015155949494104859 0.0 24-25 0.0 0.0 0.0 0.002926666109206455 0.0 26-27 0.0 0.0 0.0 0.006010117902834684 0.0 28-29 0.0 0.0 0.0 0.017559996655238733 0.0 30-31 0.0 0.0 0.0 0.04776737185383394 0.0 32-33 0.0 0.0 0.0 0.1014925997156953 0.0 34-35 0.0 0.0 0.0 0.1844844886696212 0.0 36-37 0.0 0.0 0.0 0.2979450622961786 0.0 38-39 0.0 0.0 0.0 0.44715277197090053 0.0 40-41 0.0 0.0 0.0 0.6137636926164395 0.0 42-43 0.0 0.0 0.0 0.7804268751567858 0.0 44-45 0.0 0.0 0.0 0.9809557655322352 0.0 46-47 0.0 0.0 0.0 1.227527385232879 0.0 48-49 0.0 0.0 0.0 1.4746216238815955 0.0 50-51 0.0 0.0 0.0 1.7395894305543942 0.0 52-53 0.0 0.0 0.0 1.9821368843548792 0.0 54-55 0.0 0.0 0.0 2.254943975248767 0.0 56-57 0.0 0.0 0.0 2.600238314240321 0.0 58-59 0.0 0.0 0.0 2.9565076511413997 0.0 60-61 0.0 0.0 0.0 3.3796199515009615 0.0 62-63 0.0 0.0 0.0 3.793952253532904 0.0 64-65 0.0 0.0 0.0 4.184244083953508 0.0 66-67 0.0 0.0 0.0 4.631344594029601 0.0 68-69 0.0 0.0 0.0 5.154329375365833 0.0 70-71 0.0 0.0 0.0 5.716667363491931 0.0 72-73 0.0 0.0 0.0 6.276810352036124 0.0 74-75 0.0 0.0 0.0 6.902855589932269 0.0 76-77 0.0 0.0 0.0 7.494982858098503 0.0 78-79 0.0 0.0 0.0 8.254766284806422 0.0 80-81 0.0 0.0 0.0 9.055941132201689 0.0 82-83 0.0 0.0 0.0 9.788391587925412 0.0 84-85 0.0 0.0 0.0 10.622439167154443 0.0 86-87 0.0 0.0 0.0 11.50080483318003 0.0 88-89 0.0 0.0 0.0 12.370024667614349 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 325 0.0 38.694267 94-95 CTCCGAC 210 0.0 27.117277 94-95 GTACACG 130 9.789404E-6 25.653378 1 CTTTGCG 580 0.0 25.362679 2 CGGTTAC 70 1.7915081E-5 23.730719 28-29 CGGGAGT 160 1.8751743E-6 23.72638 4 GTACATG 6295 0.0 23.537186 1 TTGCGTT 635 0.0 23.16591 4 TACATGG 6495 0.0 22.794857 2 TTTGCGT 650 0.0 22.631313 3 ACATGGG 6470 0.0 21.92948 3 AGTACTT 5140 0.0 21.883396 12-13 GAGTACT 5120 0.0 21.551748 12-13 ATGGGAG 1505 0.0 21.440447 5 CATGGGA 4870 0.0 20.851929 4 ATGGGAT 1330 0.0 20.693682 5 GCTTTGC 760 0.0 20.686653 1 GTTTTCG 115 0.0037443538 20.631632 6 TATTCCG 120 0.0047904225 19.77198 7 GTACTTT 5665 0.0 19.43648 14-15 >>END_MODULE