##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938908_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 962533 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.922560577143848 33.0 31.0 34.0 30.0 34.0 2 31.939329872326454 33.0 31.0 34.0 30.0 34.0 3 31.979719136902318 34.0 31.0 34.0 30.0 34.0 4 35.495265097404456 37.0 35.0 37.0 33.0 37.0 5 35.557801135129914 37.0 35.0 37.0 33.0 37.0 6 35.681762599308286 37.0 35.0 37.0 33.0 37.0 7 35.62968022914539 37.0 35.0 37.0 33.0 37.0 8 35.62575516891369 37.0 35.0 37.0 33.0 37.0 9 37.214046687230464 39.0 37.0 39.0 34.0 39.0 10-11 37.110242454024956 39.0 37.0 39.0 33.0 39.0 12-13 37.024320724588144 39.0 37.0 39.0 33.0 39.0 14-15 38.12484455078423 40.0 38.0 41.0 33.0 41.0 16-17 38.11286626016978 40.0 38.0 41.0 33.0 41.0 18-19 38.15968231738548 40.0 38.0 41.0 33.0 41.0 20-21 38.042964760688726 40.0 38.0 41.0 33.0 41.0 22-23 38.13253727404671 40.0 38.0 41.0 33.0 41.0 24-25 38.04016381776002 40.0 38.0 41.0 33.0 41.0 26-27 37.903407987050834 40.0 37.5 41.0 33.0 41.0 28-29 37.79972167188034 40.0 38.0 41.0 33.0 41.0 30-31 37.637422820827965 40.0 37.0 41.0 33.0 41.0 32-33 37.39673029392239 40.0 36.5 41.0 32.0 41.0 34-35 37.037289630589285 40.0 36.0 41.0 31.0 41.0 36-37 36.70881725613563 39.5 35.5 41.0 30.5 41.0 38-39 36.46414772272743 39.0 35.0 41.0 30.0 41.0 40-41 36.368531780209096 39.0 35.0 41.0 30.0 41.0 42-43 36.132844276507925 39.0 35.0 41.0 30.0 41.0 44-45 35.95889335742255 39.0 35.0 41.0 29.0 41.0 46-47 35.60796668789537 39.0 35.0 41.0 26.5 41.0 48-49 35.316406814104035 39.0 35.0 41.0 25.0 41.0 50-51 34.08745154711579 38.0 34.0 40.0 23.5 40.5 52-53 34.246071563260685 38.0 34.5 39.5 23.0 40.5 54-55 34.7882612855871 39.0 35.0 40.5 23.0 41.0 56-57 34.679589167332445 39.0 35.0 41.0 23.0 41.0 58-59 34.51885909366224 39.0 34.5 40.5 22.5 41.0 60-61 34.16780879201025 38.0 34.0 40.0 22.0 41.0 62-63 33.85894249859486 38.0 34.0 40.0 21.5 41.0 64-65 33.43074471212935 37.0 33.5 40.0 19.0 41.0 66-67 32.96049745826896 36.5 33.0 39.0 17.0 41.0 68-69 32.56363781813195 36.0 32.5 39.0 13.5 41.0 70-71 32.043388642259536 35.5 32.0 38.5 10.0 40.0 72-73 31.558760063291338 35.0 31.5 37.5 7.5 40.0 74-75 31.10693035979026 35.0 31.0 37.0 4.0 39.0 76-77 30.67651290916779 35.0 31.0 36.5 2.0 39.0 78-79 30.206332665996907 35.0 30.5 36.0 2.0 38.5 80-81 29.736008012192826 34.0 30.0 36.0 2.0 37.0 82-83 29.33912032106951 34.0 29.5 35.0 2.0 37.0 84-85 28.945908348077417 34.0 29.0 35.0 2.0 36.5 86-87 28.6099499965196 34.0 29.0 35.0 2.0 36.0 88-89 28.117421428667903 34.0 28.5 35.0 2.0 36.0 90-91 27.616432371669333 34.0 27.0 35.0 2.0 35.0 92-93 27.221424616091085 34.0 26.0 35.0 2.0 35.0 94-95 26.734752990287085 34.0 25.0 35.0 2.0 35.0 96-97 26.090899221117613 33.0 24.5 35.0 2.0 35.0 98-99 25.45288161548747 33.0 20.5 35.0 2.0 35.0 100-101 22.901701032587972 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1598509516231843 1101 2 0.28782418384352937 1101 3 0.1400101057112373 1101 4 -0.058143138641163716 1101 5 -0.12154026810418372 1101 6 0.21898093325451384 1101 7 0.1556169823812681 1101 8 0.2891919246232959 1101 9 0.17370784643393478 1101 10-11 0.23957350971404878 1101 12-13 0.39662271028191753 1101 14-15 0.19274109138063267 1101 16-17 0.34439050673462646 1101 18-19 0.35888451273393684 1101 20-21 0.47685428485593206 1101 22-23 0.42617991443890446 1101 24-25 0.4469361778025345 1101 26-27 0.4319427217115219 1101 28-29 0.34403046784006364 1101 30-31 0.24111219264554506 1101 32-33 0.35039995086051334 1101 34-35 -0.13896119841228938 1101 36-37 -0.21732001258094868 1101 38-39 -0.11911884928850469 1101 40-41 -0.2171630350906142 1101 42-43 -0.21883276882992675 1101 44-45 -0.044965043395365 1101 46-47 -0.04915187873071858 1101 48-49 0.030188796595105316 1101 50-51 -0.0023458938198004375 1101 52-53 0.07117366289781302 1101 54-55 0.053324853713682785 1101 56-57 0.031872671697570354 1101 58-59 -0.12901763151995738 1101 60-61 -0.08381740792772518 1101 62-63 -0.14766331501392216 1101 64-65 0.04471013952927194 1101 66-67 0.003078745703845698 1101 68-69 -0.30864017006372535 1101 70-71 -0.38721971753344775 1101 72-73 -0.3052502551455376 1101 74-75 -0.441421169297584 1101 76-77 -0.4337994531220062 1101 78-79 -0.3486147738279506 1101 80-81 -0.4703871544118492 1101 82-83 -0.6018800684643288 1101 84-85 -0.7373373075877758 1101 86-87 -0.7895238773436297 1101 88-89 -0.7352013856341912 1101 90-91 -0.6575675166800714 1101 92-93 -0.5077235760886722 1101 94-95 -0.5018367401068922 1101 96-97 -0.8332557754730203 1101 98-99 -0.9399286994778606 1101 100-101 -0.6169903381811714 1102 1 0.4190411348611036 1102 2 0.3605091094639086 1102 3 0.36558993110569915 1102 4 0.4763247365592349 1102 5 0.44668671146414596 1102 6 0.4916002578435865 1102 7 0.41647134682042264 1102 8 0.3293839983405533 1102 9 0.5362450807402226 1102 10-11 0.4258117863902058 1102 12-13 0.5248898895519929 1102 14-15 0.5601176730837878 1102 16-17 0.7637578127570848 1102 18-19 0.5187488864157146 1102 20-21 0.6338193726481691 1102 22-23 0.7188266236445315 1102 24-25 0.6942075690148002 1102 26-27 0.567482588958427 1102 28-29 0.584617800484331 1102 30-31 0.7010663227771445 1102 32-33 0.7487011892706761 1102 34-35 0.9138196623596997 1102 36-37 0.8496203731175171 1102 38-39 0.9322724392505037 1102 40-41 0.7917153525479179 1102 42-43 0.7212064900818334 1102 44-45 1.0075666351646717 1102 46-47 1.2795010244327543 1102 48-49 1.0711786347919414 1102 50-51 1.1575375499292235 1102 52-53 1.0389795753814681 1102 54-55 1.0964454540902508 1102 56-57 1.132883639528913 1102 58-59 1.0147376206943903 1102 60-61 1.034086461134649 1102 62-63 1.0116162432724636 1102 64-65 1.0713325890666994 1102 66-67 1.0243800108121022 1102 68-69 1.0814259776428443 1102 70-71 1.0096677932252476 1102 72-73 0.8716869140682526 1102 74-75 0.7709386498164683 1102 76-77 0.7987352257952729 1102 78-79 0.5877407837617454 1102 80-81 0.677220887054105 1102 82-83 0.7541504588993071 1102 84-85 0.5625668335408953 1102 86-87 0.6622568219684482 1102 88-89 0.7206901845061537 1102 90-91 0.7973158825721285 1102 92-93 0.9000167099369065 1102 94-95 0.6909076078430125 1102 96-97 0.40457275411037585 1102 98-99 0.2374623373362681 1102 100-101 0.5375712854697774 1103 1 -3.416531672009171E-4 1103 2 0.2048989824833889 1103 3 0.0824799258496931 1103 4 0.28368913232496595 1103 5 0.4140358261211503 1103 6 0.1936748181683825 1103 7 0.033508534597032735 1103 8 0.025147610574926205 1103 9 0.10116794484947178 1103 10-11 0.12728084526617067 1103 12-13 0.13979721037662785 1103 14-15 0.14333167477420972 1103 16-17 0.20207968733962645 1103 18-19 0.29583633705487244 1103 20-21 0.2230702862062799 1103 22-23 0.28745241001612953 1103 24-25 0.3916840636982428 1103 26-27 0.3890350697041072 1103 28-29 0.38767127562568504 1103 30-31 0.3515371781821486 1103 32-33 0.21862711834087634 1103 34-35 0.2186191040499068 1103 36-37 0.43602293295291616 1103 38-39 0.3295737754735981 1103 40-41 0.575139344637698 1103 42-43 0.5335216404771543 1103 44-45 0.701109867343888 1103 46-47 0.5161646989651132 1103 48-49 0.33554431272259677 1103 50-51 0.5221149660984921 1103 52-53 0.6114231420221969 1103 54-55 0.5470427781483878 1103 56-57 0.6946628899306049 1103 58-59 0.4456399177296859 1103 60-61 0.4475059962343835 1103 62-63 0.5612461383147149 1103 64-65 0.5426929947651473 1103 66-67 0.671931350468455 1103 68-69 0.6552515857348382 1103 70-71 0.7133559836997776 1103 72-73 0.7077951502612585 1103 74-75 0.6789941911534925 1103 76-77 0.7742280228249463 1103 78-79 0.7712832543824426 1103 80-81 0.6372746455951095 1103 82-83 0.6476995588245238 1103 84-85 0.6416365835624305 1103 86-87 0.7398377874998125 1103 88-89 0.5252892489127774 1103 90-91 0.560076669549229 1103 92-93 0.34323191014564713 1103 94-95 0.1918239749841355 1103 96-97 0.3887647966977248 1103 98-99 0.2724796721560949 1103 100-101 0.5691803337838017 1104 1 0.20388978511613232 1104 2 0.18971891277898223 1104 3 0.09669986966819621 1104 4 0.040581815821497 1104 5 0.14836001920357234 1104 6 0.21017767334427617 1104 7 0.1687805600200889 1104 8 0.2720548619226051 1104 9 0.3518161220240472 1104 10-11 0.2468970407827129 1104 12-13 0.33771898654804033 1104 14-15 0.32635797681431455 1104 16-17 0.21135762595795882 1104 18-19 0.3988371914562592 1104 20-21 0.29421856480259834 1104 22-23 0.32455371497596275 1104 24-25 0.41544687203573005 1104 26-27 0.3370598043171995 1104 28-29 0.3843624679151816 1104 30-31 0.6393395225612366 1104 32-33 0.5070164795067882 1104 34-35 0.13790583255310196 1104 36-37 0.2785487647649276 1104 38-39 0.2584279555863347 1104 40-41 0.46125132654159273 1104 42-43 0.3507989890053125 1104 44-45 0.29820059985478764 1104 46-47 0.33327915801838515 1104 48-49 0.2800770658005902 1104 50-51 0.4715147337504817 1104 52-53 0.49356676751163775 1104 54-55 0.27357347820431954 1104 56-57 0.33489597554899575 1104 58-59 0.11118479839009154 1104 60-61 0.2815892358294292 1104 62-63 0.23295561725279867 1104 64-65 0.3449617685454811 1104 66-67 0.4613177181561028 1104 68-69 0.15084004701324005 1104 70-71 0.02140702649975168 1104 72-73 -0.07122775876035803 1104 74-75 -0.17720882445316732 1104 76-77 -0.09989713775948772 1104 78-79 0.07650493773759237 1104 80-81 -0.07394751720831039 1104 82-83 -0.2601584753146504 1104 84-85 -0.3334351800081059 1104 86-87 -0.1842434236602699 1104 88-89 -0.07603476591323499 1104 90-91 0.05147610527550128 1104 92-93 0.053818496658401216 1104 94-95 0.21974138694698198 1104 96-97 0.12923084499906778 1104 98-99 -0.001957129801034796 1104 100-101 0.2539277321622819 1105 1 -0.007920544515457095 1105 2 0.07894859724928338 1105 3 0.056527121563132 1105 4 0.09311134164557444 1105 5 0.044605392022184276 1105 6 0.188178730985733 1105 7 0.12042871796121091 1105 8 0.32952004789122924 1105 9 0.30906977970075644 1105 10-11 0.10273169616787214 1105 12-13 0.12695748469717216 1105 14-15 0.09669113387407435 1105 16-17 0.32151979764842054 1105 18-19 0.376670789199963 1105 20-21 0.19009376311037585 1105 22-23 0.25496090693788176 1105 24-25 0.3040067169149836 1105 26-27 0.21300727479137294 1105 28-29 0.23475026978368163 1105 30-31 0.06470529023265925 1105 32-33 -0.048580206549971194 1105 34-35 -0.06620134178146486 1105 36-37 -0.13067034329078098 1105 38-39 0.05316618932509698 1105 40-41 0.004667508455931113 1105 42-43 0.04568705528417638 1105 44-45 -0.08775039609092516 1105 46-47 -0.07107479932341221 1105 48-49 -0.22681523104410672 1105 50-51 -0.42603506353490417 1105 52-53 -0.45180605110285654 1105 54-55 -0.7264353354745055 1105 56-57 -0.6829076556866767 1105 58-59 -0.5482634653151734 1105 60-61 -0.2641096889017547 1105 62-63 -0.37265869616161496 1105 64-65 -0.1886712298680635 1105 66-67 -0.28090861711623916 1105 68-69 -0.491315778828465 1105 70-71 -0.5888344683001776 1105 72-73 -0.39607351938083823 1105 74-75 -0.29358463971845694 1105 76-77 -0.519560455228202 1105 78-79 -0.5486757426784088 1105 80-81 -0.6421918323854676 1105 82-83 -0.8214399842164646 1105 84-85 -1.00539886765133 1105 86-87 -0.6994470196412301 1105 88-89 -0.3501313638332064 1105 90-91 -0.3675349999598261 1105 92-93 -0.45124055498975935 1105 94-95 -0.3130014299047694 1105 96-97 -0.6038909600423601 1105 98-99 -0.5855019914262769 1105 100-101 -0.21192807605206454 1106 1 0.14354477279426447 1106 2 0.14679595124464484 1106 3 0.21513981395191806 1106 4 0.09980893107561428 1106 5 0.16786071565701377 1106 6 0.05147200528470108 1106 7 0.13131493624781143 1106 8 0.11877438761995052 1106 9 2.3570843784170847E-4 1106 10-11 0.24023608860585455 1106 12-13 0.29839258203359975 1106 14-15 0.3090180718177109 1106 16-17 0.151434057259074 1106 18-19 0.011339423678840888 1106 20-21 0.10569218886060838 1106 22-23 0.17210898480702497 1106 24-25 0.07238819136856733 1106 26-27 0.2109445859165433 1106 28-29 0.24914692450291653 1106 30-31 0.22879271731819273 1106 32-33 0.1918302228547617 1106 34-35 0.3343503484286714 1106 36-37 0.2593605794725917 1106 38-39 0.2733749179778542 1106 40-41 0.19967769891203346 1106 42-43 0.4689353510182386 1106 44-45 0.37621052878081684 1106 46-47 0.44982617755479737 1106 48-49 0.23772198169044856 1106 50-51 -0.07243326439230913 1106 52-53 -0.05309918484380205 1106 54-55 -0.14995771933845248 1106 56-57 -0.12938667740289134 1106 58-59 0.20970617415351 1106 60-61 -0.1263628338873488 1106 62-63 -0.05184205409261011 1106 64-65 -0.3004148520481138 1106 66-67 -0.36641005783589975 1106 68-69 -0.2034529634514186 1106 70-71 0.051575961104560974 1106 72-73 -0.23257994991004338 1106 74-75 -0.0664745125134516 1106 76-77 0.030977179180400327 1106 78-79 -0.04319398008089692 1106 80-81 -0.0804763218084723 1106 82-83 -0.21418719062591052 1106 84-85 -0.10890930408396926 1106 86-87 0.15962600024772655 1106 88-89 0.14489991032520066 1106 90-91 0.019301741905380254 1106 92-93 0.19070195678197877 1106 94-95 0.3991638663212491 1106 96-97 0.5626070370059217 1106 98-99 0.6418997936605138 1106 100-101 0.40583494313410284 1107 1 0.06953294584125658 1107 2 0.12619215417025842 1107 3 0.262074143239186 1107 4 0.13444945395143293 1107 5 0.20975302813756258 1107 6 0.22931203018355006 1107 7 0.07784465924812167 1107 8 0.17669533377619473 1107 9 0.1756169720543994 1107 10-11 0.20917477519159888 1107 12-13 0.033997935286009806 1107 14-15 -0.005049777544250844 1107 16-17 0.2429995723540017 1107 18-19 0.11975834680364272 1107 20-21 0.017845939249653497 1107 22-23 0.14154564836133687 1107 24-25 0.16753291592505093 1107 26-27 0.2976548255679603 1107 28-29 0.33274480810079154 1107 30-31 0.2786837848563195 1107 32-33 0.2531436371093605 1107 34-35 0.13987478042368906 1107 36-37 0.15405401240132477 1107 38-39 0.20733072252188833 1107 40-41 0.1916789438544626 1107 42-43 0.30211306654889825 1107 44-45 0.3904028723849251 1107 46-47 0.14216978166310668 1107 48-49 0.09878640746075718 1107 50-51 0.240358518987712 1107 52-53 0.15135148431274814 1107 54-55 0.30371486080472465 1107 56-57 0.2263089341750728 1107 58-59 0.2603130835839522 1107 60-61 0.21703897127916605 1107 62-63 -0.12042281973219104 1107 64-65 0.1427846499749137 1107 66-67 -0.0339637459778217 1107 68-69 0.013898421547025919 1107 70-71 0.19172535419512826 1107 72-73 -0.01325221881760541 1107 74-75 0.12283454444266084 1107 76-77 0.06774966595288845 1107 78-79 0.1750911117336038 1107 80-81 0.006545153448296759 1107 82-83 0.17160490398971362 1107 84-85 0.34555692685285067 1107 86-87 0.19588494827138092 1107 88-89 0.07124600431835404 1107 90-91 0.07754948544723916 1107 92-93 0.2057657600810714 1107 94-95 0.27222642467792113 1107 96-97 0.2381127948646231 1107 98-99 0.32143605260854 1107 100-101 0.5216612736663144 1108 1 0.25199492031187276 1108 2 0.15032508067899286 1108 3 0.15825730572904817 1108 4 0.1788379028755145 1108 5 0.2707207221506138 1108 6 0.30319069131923015 1108 7 0.0999080370350427 1108 8 0.018337716671275928 1108 9 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2316 80-81 -1.9975042991160095 2316 82-83 -2.0786714331678127 2316 84-85 -2.4284346389703977 2316 86-87 -2.456486836526146 2316 88-89 -2.6214264772155147 2316 90-91 -2.8895886569384253 2316 92-93 -2.9715588771445525 2316 94-95 -3.164521178653839 2316 96-97 -3.5934629817606556 2316 98-99 -3.6215900357145756 2316 100-101 -3.912630726962316 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3168.0 3 653.0 4 600.0 5 675.0 6 748.0 7 1016.0 8 1225.0 9 1526.0 10 2119.0 11 2902.0 12 4196.0 13 6484.0 14 6467.0 15 6659.0 16 8947.0 17 12293.0 18 10419.0 19 6277.0 20 5911.0 21 6340.0 22 6823.0 23 7328.0 24 8357.0 25 9611.0 26 11226.0 27 13167.0 28 15438.0 29 18786.0 30 22037.0 31 27067.0 32 33297.0 33 42547.0 34 57365.0 35 83664.0 36 123736.0 37 186274.0 38 177164.0 39 30013.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.4056849779604 19.422355065254106 14.942236548340754 19.229723408444745 2 14.532747840536054 23.632807422712144 38.48311890335521 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29.63324835021296 21.855394885517182 22-23 20.247074088423506 26.965034674099865 30.456054771296554 22.33183646618008 24-25 22.491784827494378 26.986022504215512 30.24899852392152 20.27319414436859 26-27 21.94366252858067 25.083999608868695 33.48078775509355 19.491550107457083 28-29 19.34400753913893 28.99557200408162 31.83861166673428 19.821808790045175 30-31 20.079328593241 27.45651332786933 30.965090010442626 21.499068068447045 32-33 20.395092647291303 27.753365588624394 31.20616206411675 20.64537969996755 34-35 22.29249341642114 26.3109535108075 30.634161604046618 20.762391468724744 36-37 21.616483398185395 25.66087962600061 32.74668606480189 19.9759509110121 38-39 21.282614215717523 26.929997711558094 32.081970108141945 19.70541796458244 40-41 20.24540582209488 27.900001357910824 31.720535685374152 20.134057134620146 42-43 19.444160012705392 28.618581217335336 29.51448895278735 22.422769817171922 44-45 20.72643342289777 26.92801297586986 29.649852171650192 22.695701429582183 46-47 22.25721691525496 28.173230000297817 29.055754592226197 20.513798492221024 48-49 21.667582085933045 27.455209345324544 29.59070568112158 21.28650288762083 50-51 19.63946705461295 28.441729935249967 31.536625755682678 20.382177254454405 52-53 20.678951378502695 29.739684092479195 28.66234182937064 20.91902269964747 54-55 19.166189429154265 27.479893321740295 30.177521904156773 23.176395344948666 56-57 21.656554681766302 27.787107676315493 28.446584952426225 22.10975268949198 58-59 22.96416819012797 26.995560198485247 28.57508487855837 21.465186732828414 60-61 21.001943077538296 27.477006219727514 31.105580840274015 20.41546986246018 62-63 20.945759552266566 27.504237208437214 30.36646889544179 21.18353434385443 64-65 20.286802566359285 29.44733172639717 29.590847989831566 20.675017717411983 66-67 20.74146285367804 27.55779855836831 28.433493108080558 23.267245479873086 68-69 19.697283328001607 28.311159619377378 29.559463398213136 22.432093654407876 70-71 22.02040824860387 28.122183960676328 27.993129648098318 21.86427814262148 72-73 21.15980038801826 26.488361542707096 30.36749887106755 21.98433919820709 74-75 19.879813082060654 30.233365197117312 28.473888508811495 21.412933212010536 76-77 21.07849771568047 27.268708401896014 28.622003773843225 23.030790108580295 78-79 20.898847172367244 27.564841656640525 29.759273374315566 21.77703779667667 80-81 21.75791144778489 28.13956975013621 28.62998835595364 21.47253044612526 82-83 20.805263229391656 27.505984979816695 29.818731654508618 21.870020136283035 84-85 20.49705344144871 27.925004101877036 29.087533533497968 22.490408923176282 86-87 20.807858413959647 28.519300680155528 28.7630866459001 21.909754259984723 88-89 21.007885651521192 27.782146792988975 27.8870728300611 23.322894725428732 90-91 20.951759777237037 26.549316826798453 29.0607309200679 23.438192475896614 92-93 21.809058883789973 28.438790336911868 28.82896208565485 20.92318869364331 94-95 21.104424534544233 27.790408752272267 28.77076401541641 22.33440269776709 96-97 19.462035653491423 27.635610843954694 29.854063085847365 23.048290416706518 98-99 21.232430658200908 28.323357424647565 27.41502010164765 23.02919181550388 100-101 21.156312149831585 26.642688959002403 28.84603928888313 23.354959602282882 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3014.0 1 1866.0 2 783.5 3 804.5 4 699.0 5 728.5 6 1075.0 7 1992.0 8 3133.5 9 3501.5 10 2961.5 11 2407.5 12 2334.0 13 2422.0 14 2429.5 15 2477.5 16 2815.5 17 2995.5 18 2888.0 19 2899.0 20 3069.0 21 3340.5 22 3682.5 23 4197.0 24 4669.5 25 5374.5 26 6740.0 27 8071.0 28 8849.5 29 9981.0 30 11576.0 31 12952.0 32 15202.0 33 18017.0 34 20780.0 35 22725.0 36 24522.5 37 26849.5 38 26963.0 39 25413.0 40 24359.5 41 24373.5 42 24598.0 43 25431.0 44 26731.0 45 27206.0 46 30861.0 47 48834.5 48 94668.0 49 93247.0 50 45938.5 51 27114.5 52 20988.0 53 18547.5 54 17211.5 55 15690.5 56 14097.0 57 12702.5 58 11688.5 59 10583.5 60 9268.5 61 8006.0 62 6662.5 63 4961.5 64 3529.5 65 2636.0 66 1833.0 67 1315.5 68 934.0 69 665.0 70 506.5 71 372.5 72 289.0 73 272.0 74 211.5 75 164.5 76 131.5 77 70.5 78 32.5 79 25.0 80 24.0 81 16.0 82 10.5 83 7.0 84 4.0 85 4.5 86 4.0 87 2.5 88 1.5 89 1.0 90 0.5 91 1.0 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27739308678247915 2 0.08685416500005715 3 0.09049040396537053 4 0.10513925236849023 5 0.10680153303834777 6 0.08425685145340471 7 0.09204879209336199 8 0.08810087550245031 9 0.09371107276321955 10-11 0.08716584262565544 12-13 0.09215268463522809 14-15 0.1004121417135828 16-17 0.08924369346297738 18-19 0.09937321629492184 20-21 0.10072381933918109 22-23 0.11225589148631787 24-25 0.12487883532304865 26-27 0.1266450085347723 28-29 0.11947642314601162 30-31 0.11313897809217971 32-33 0.10773656591514263 34-35 0.3852335452394879 36-37 0.5963431903113972 38-39 0.5766036073568387 40-41 0.5381633668663828 42-43 0.5671493860470238 44-45 0.5901096377994313 46-47 0.5779022641301649 48-49 0.6001352680895097 50-51 0.5921355423658202 52-53 0.5957198350602005 54-55 0.585642258499189 56-57 0.5543186571265609 58-59 0.5488642986785908 60-61 0.49598299486874736 62-63 0.5750452192288472 64-65 0.6074596922910694 66-67 0.6210696152755282 68-69 0.6228357884872519 70-71 0.6190956569800724 72-73 0.6105764685470524 74-75 0.6084466714387974 76-77 0.5904732616959626 78-79 0.5663701919830282 80-81 0.5601366394710623 82-83 0.577019177524303 84-85 0.5874084317109128 86-87 0.5761880371893743 88-89 0.5889148735679712 90-91 0.604758486202551 92-93 0.6132776746355709 94-95 0.6119270715913117 96-97 0.608031101271333 98-99 0.6124465343006422 100-101 0.5892784974645026 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 962533.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.02917507834958 #Duplication Level Percentage of deduplicated Percentage of total 1 75.03918718901502 30.787959489151444 2 12.90247239096774 10.587555973451744 3 4.766770514252163 5.867299859626994 4 2.2620012362572424 3.712321789993664 5 1.246614749044154 2.557378739689271 6 0.8046961167307503 1.9809610714928392 7 0.5187635214571283 1.4899107542287973 8 0.3659543960507783 1.2011845589007253 9 0.2806013156282393 1.0361556455513634 >10 1.5947702662784717 12.152052067130366 >50 0.13201622867947577 3.817755335676889 >100 0.0701886028018794 5.071948380513405 >500 0.004814380696850619 1.4414496841160984 >1k 0.008108430647327358 8.111520281557183 >5k 0.0025338845772897994 6.666016032877037 >10k+ 5.067769154579599E-4 3.5185303360422098 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21375 2.2207030823878244 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12469 1.2954361045283642 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9061 0.9413703218487054 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7528 0.7821030551679786 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7247 0.7529092509036054 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7081 0.7356630889538333 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5978 0.6210696152755282 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5933 0.6163944508915539 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5708 0.593018628971682 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5399 0.560915833535058 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5149 0.5349426980685338 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5035 0.5230989482957987 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4999 0.5193588167886192 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4925 0.511670768690528 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4504 0.46793200856490114 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4473 0.4647113397670521 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3773 0.3919865604607842 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3553 0.3691302012502428 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3120 0.3241447306222228 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3041 0.31593721981480116 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2878 0.29900273549062734 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2784 0.2892368365552142 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2757 0.28643173792482957 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2628 0.2730296000241031 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2598 0.26991282376812015 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2532 0.26305591600495776 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2207 0.2292908398984762 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2196 0.22814802193794914 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2057 0.21370695861856168 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2014 0.2092395793183195 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1983 0.20601891052047047 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1958 0.203421596973818 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1928 0.20030482071783512 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1818 0.18887664111256444 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1715 0.17817570930035645 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1612 0.16747477748814846 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1610 0.16726699240441628 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1287 0.1337097013816669 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1239 0.12872285937209424 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1232 0.12799561157903158 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1221 0.12685279361850452 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1220 0.12674890107663841 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1109 0.11521682892950164 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1052 0.10929495404313411 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 989 0.10274972390557 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 966 0.10036019544264975 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.194627093304853E-5 0.0 14-15 0.0 0.0 0.0 1.0389254186609706E-4 0.0 16-17 0.0 0.0 0.0 3.636238965313397E-4 0.0 18-19 0.0 0.0 0.0 5.194627093304852E-4 0.0 20-21 0.0 0.0 0.0 6.753015221296309E-4 0.0 22-23 0.0 0.0 0.0 0.001870065753589747 0.0 24-25 0.0 0.0 0.0 0.003844024049045591 0.0 26-27 0.0 0.0 0.0 0.006493283866631066 0.0 28-29 0.0 0.0 0.0 0.017350054491638206 0.0 30-31 0.0 0.0 0.0 0.04586855723388185 0.0 32-33 0.0 0.0 0.0 0.10311334780210132 0.0 34-35 0.0 0.0 0.0 0.18451315435418836 0.0 36-37 0.0 0.0 0.0 0.29541844279624696 0.0 38-39 0.0 0.0 0.0 0.4534909452455137 0.0 40-41 0.0 0.0 0.0 0.6271473289746949 0.0 42-43 0.0 0.0 0.0 0.8057905547134487 0.0 44-45 0.0 0.0 0.0 1.0140431548840403 0.0 46-47 0.0 0.0 0.0 1.2692551839781077 0.0 48-49 0.0 0.0 0.0 1.5100261497527876 0.0 50-51 0.0 0.0 0.0 1.7732379045705446 0.0 52-53 0.0 0.0 0.0 2.0245020170737003 0.0 54-55 0.0 0.0 0.0 2.2927006139010295 0.0 56-57 0.0 0.0 0.0 2.6391822410244634 0.0 58-59 0.0 0.0 0.0 2.991741581847064 0.0 60-61 0.0 0.0 0.0 3.4109999345476987 0.0 62-63 0.0 0.0 0.0 3.817323665786004 0.0 64-65 0.0 0.0 0.0 4.2059856649070735 0.0 66-67 0.0 0.0 0.0 4.662541440137637 0.0 68-69 0.0 0.0 0.0 5.192549242467531 0.0 70-71 0.0 0.0 0.0 5.767490569154512 0.0 72-73 0.0 0.0 0.0 6.341756594319364 0.0 74-75 0.0 0.0 0.0 6.962150908072762 0.0 76-77 0.0 0.0 0.0 7.556883763985235 0.0 78-79 0.0 0.0 0.0 8.327974209715407 0.0 80-81 0.0 0.0 0.0 9.13262194646833 0.0 82-83 0.0 0.0 0.0 9.873479662515468 0.0 84-85 0.0 0.0 0.0 10.728463335802513 0.0 86-87 0.0 0.0 0.0 11.600277600871866 0.0 88-89 0.0 0.0 0.0 12.463312946153534 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGC 95 0.0012524744 24.909962 6 GAGTACT 4905 0.0 24.894728 12-13 GTACACG 120 1.733208E-4 23.703907 1 CGTAGAG 140 1.8279903E-5 23.666925 2 GACCGCG 100 0.0016885363 23.664467 7 AGTACTT 4980 0.0 22.999199 12-13 GTATAGG 250 3.929017E-10 22.75575 1 GTACTTT 5445 0.0 22.556211 14-15 TATATGG 315 0.0 22.53993 2 GTAAGCG 95 3.2437129E-7 22.532614 94-95 CGTCACT 110 0.002909638 21.549006 1 TAGCACC 110 0.0029361174 21.515387 4 GTACATG 6430 0.0 21.30771 1 TTTGCGT 695 0.0 21.112942 3 ATGGGAG 1450 0.0 20.892183 5 TACATGG 6630 0.0 20.347132 2 ACATGGG 6560 0.0 19.914852 3 ATGGGAT 1410 0.0 19.806364 5 ACTTTTT 6260 0.0 19.73299 16-17 TTGCGTT 745 0.0 19.695967 4 >>END_MODULE