##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938908_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 962533 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14194734102623 33.0 31.0 34.0 30.0 34.0 2 32.349679439562074 34.0 31.0 34.0 30.0 34.0 3 32.43178155969717 34.0 31.0 34.0 30.0 34.0 4 35.98598074040059 37.0 35.0 37.0 35.0 37.0 5 35.91886304157883 37.0 35.0 37.0 35.0 37.0 6 35.99887172699533 37.0 35.0 37.0 35.0 37.0 7 35.950647925837345 37.0 35.0 37.0 35.0 37.0 8 35.94360297257341 37.0 35.0 37.0 35.0 37.0 9 37.58535135938197 39.0 37.0 39.0 35.0 39.0 10-11 37.50697482579818 39.0 37.0 39.0 34.5 39.0 12-13 37.45461246523496 39.0 37.0 39.0 34.0 39.0 14-15 38.573640592062816 40.0 38.0 41.0 34.0 41.0 16-17 38.605571964805364 40.0 38.0 41.0 34.0 41.0 18-19 38.80779256399521 40.0 38.0 41.0 34.0 41.0 20-21 38.81416169627431 40.0 38.0 41.0 34.0 41.0 22-23 38.756880023853725 40.0 38.0 41.0 34.0 41.0 24-25 38.65618841120252 40.0 38.0 41.0 34.0 41.0 26-27 38.435057811004924 40.0 38.0 41.0 34.0 41.0 28-29 38.26965672865242 40.0 38.0 41.0 34.0 41.0 30-31 38.12508298416782 40.0 37.5 41.0 34.0 41.0 32-33 37.91118798004848 40.0 37.0 41.0 33.0 41.0 34-35 37.8103363728828 40.0 37.0 41.0 33.0 41.0 36-37 37.65191219417932 40.0 37.0 41.0 33.0 41.0 38-39 37.473719342609556 40.0 36.5 41.0 33.0 41.0 40-41 37.35957468471211 40.0 36.0 41.0 32.5 41.0 42-43 37.15244100721742 40.0 36.0 41.0 31.5 41.0 44-45 36.85180560043136 40.0 36.0 41.0 31.0 41.0 46-47 36.43687800833842 40.0 36.0 41.0 30.0 41.0 48-49 36.04230867928684 39.0 35.0 41.0 29.0 41.0 50-51 35.748388886407014 39.0 35.0 41.0 28.0 41.0 52-53 35.543920052611185 39.0 35.0 41.0 27.5 41.0 54-55 35.351244580705284 39.0 35.0 40.0 27.0 41.0 56-57 35.076944374894154 38.5 35.0 40.0 26.5 41.0 58-59 34.80967717470466 38.0 34.5 40.0 26.0 41.0 60-61 34.304909545958424 38.0 34.0 40.0 24.5 41.0 62-63 34.028681614032976 37.5 34.0 40.0 24.0 41.0 64-65 33.77021411213953 37.0 33.5 39.5 23.5 41.0 66-67 33.27564717261642 36.5 33.0 39.0 22.0 40.5 68-69 32.82646049538042 36.0 32.5 39.0 21.5 40.0 70-71 32.29063055500435 35.0 32.0 38.0 20.5 40.0 72-73 31.740333058710714 35.0 31.5 37.0 18.0 39.5 74-75 31.3103976694825 35.0 31.0 37.0 16.0 39.0 76-77 29.305806658057435 33.0 28.5 35.0 9.5 37.0 78-79 30.270990708889983 34.0 30.0 36.0 9.0 37.5 80-81 30.248978996044812 34.0 30.5 35.5 7.0 37.0 82-83 29.925032180714844 34.0 30.0 35.0 4.0 37.0 84-85 29.53074803669069 34.0 30.0 35.0 2.0 36.0 86-87 29.04530857643322 34.0 29.0 35.0 2.0 36.0 88-89 28.453135113289623 34.0 29.0 35.0 2.0 35.0 90-91 27.957859107168275 33.0 27.5 35.0 2.0 35.0 92-93 27.657870431455336 33.0 27.0 35.0 2.0 35.0 94-95 27.294092773962035 33.0 27.0 35.0 2.0 35.0 96-97 26.680925225420843 33.0 25.0 35.0 2.0 35.0 98-99 25.88347828074466 33.0 24.5 34.5 2.0 35.0 100-101 21.7419413152588 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13244189419319952 1101 2 0.14023536397489522 1101 3 0.16461275668413577 1101 4 0.08387338896476848 1101 5 0.1528646236135458 1101 6 0.07987156198142742 1101 7 0.0448147428809591 1101 8 0.09394874996758062 1101 9 0.1983626011147379 1101 10-11 0.3032702226508519 1101 12-13 0.16156184522491657 1101 14-15 0.33071752786448627 1101 16-17 0.31478383620130046 1101 18-19 0.26628860488151673 1101 20-21 0.23723989503222498 1101 22-23 0.17537690997610156 1101 24-25 0.21466870091239088 1101 26-27 0.24872733486771637 1101 28-29 0.27349790370049476 1101 30-31 0.30719565439365937 1101 32-33 0.27879185363018877 1101 34-35 0.45984699230668724 1101 36-37 0.2397007336103485 1101 38-39 0.46052501668271617 1101 40-41 0.3210560491856356 1101 42-43 0.3644322678002325 1101 44-45 0.3639692245998134 1101 46-47 0.33432181129182226 1101 48-49 0.29017314980880826 1101 50-51 0.19780806074807344 1101 52-53 0.1664552436564648 1101 54-55 0.27893429816774784 1101 56-57 0.08624818155873015 1101 58-59 0.1877608565782367 1101 60-61 0.5393725314491391 1101 62-63 0.39065464306271735 1101 64-65 0.22369747505927506 1101 66-67 0.37757445880637874 1101 68-69 0.4118650585393553 1101 70-71 0.23447513526195962 1101 72-73 0.24472174631137733 1101 74-75 0.10417264266450132 1101 76-77 -0.16929125934330003 1101 78-79 0.03459538697352471 1101 80-81 0.20272106204075868 1101 82-83 0.4310135837598814 1101 84-85 0.138051385991254 1101 86-87 0.15474780819909384 1101 88-89 0.34907541584797386 1101 90-91 0.4521294518613992 1101 92-93 0.4448522904948149 1101 94-95 0.5690335957349326 1101 96-97 0.5156794192112635 1101 98-99 0.40623070513681725 1101 100-101 0.6483286331388065 1102 1 0.19897863013505912 1102 2 0.19539108378729964 1102 3 0.19232537839884145 1102 4 0.1960193690581562 1102 5 0.16705656765433474 1102 6 0.10058038366739908 1102 7 0.10374506466927613 1102 8 0.19220027906914083 1102 9 0.19925635159943766 1102 10-11 0.3809298931455203 1102 12-13 0.35563474119261684 1102 14-15 0.362101773192002 1102 16-17 0.3311523105832066 1102 18-19 0.23742987212561673 1102 20-21 0.28428002989573287 1102 22-23 0.30504510351948966 1102 24-25 0.2854504338221062 1102 26-27 0.2016312611402853 1102 28-29 0.40510234473774887 1102 30-31 0.3383904703865852 1102 32-33 0.306078577176514 1102 34-35 0.5342984418225001 1102 36-37 0.599377457830478 1102 38-39 0.530800121472744 1102 40-41 0.4192471897409362 1102 42-43 0.5464795793657231 1102 44-45 0.7555158451931945 1102 46-47 0.9251868676820152 1102 48-49 0.7916313742838383 1102 50-51 0.7468143195441428 1102 52-53 0.8963480825919561 1102 54-55 0.8969449596066781 1102 56-57 0.9020936275631115 1102 58-59 0.7700520107368476 1102 60-61 0.7832647067462872 1102 62-63 0.8920543859982075 1102 64-65 0.8656194581428522 1102 66-67 0.8950553537773516 1102 68-69 0.8823522224770883 1102 70-71 1.0556455797089512 1102 72-73 1.2204970398950579 1102 74-75 1.1312387890997329 1102 76-77 0.8661224911922538 1102 78-79 0.7945420162120058 1102 80-81 1.1386055889696003 1102 82-83 0.9496958929670356 1102 84-85 0.9255917668586378 1102 86-87 1.0547481896004385 1102 88-89 1.3819452194135664 1102 90-91 1.2653870897411643 1102 92-93 1.2813633326994172 1102 94-95 1.5732372742237715 1102 96-97 1.3271095089804277 1102 98-99 1.4164935796570468 1102 100-101 1.8186675590871921 1103 1 -0.08308142312409927 1103 2 0.044426463798757254 1103 3 -0.036435565883792265 1103 4 0.06574292331421105 1103 5 0.13006979255505513 1103 6 -0.07236456314210926 1103 7 0.08087209138250273 1103 8 0.03675744527242131 1103 9 -0.0037551754003075644 1103 10-11 0.11074016519669527 1103 12-13 0.0023040462642214266 1103 14-15 0.08583705544931064 1103 16-17 0.04987816075093576 1103 18-19 0.08695277443763416 1103 20-21 0.11439442597357896 1103 22-23 0.08905587020917238 1103 24-25 0.03197504277429175 1103 26-27 -0.028427453773396394 1103 28-29 0.05628794154370098 1103 30-31 0.2021701227913013 1103 32-33 0.24148530560848513 1103 34-35 0.09897972958050616 1103 36-37 0.04193035181529581 1103 38-39 -7.040564872582422E-4 1103 40-41 0.11076854646368162 1103 42-43 0.19194287536603838 1103 44-45 0.25021926808663864 1103 46-47 0.28185156594878435 1103 48-49 0.21814284947263474 1103 50-51 0.37571559034810775 1103 52-53 0.5127566724727117 1103 54-55 0.2962588078617685 1103 56-57 0.2968069132636586 1103 58-59 0.4921280941786961 1103 60-61 0.2522099731606815 1103 62-63 0.3499258424102152 1103 64-65 0.41709082666351804 1103 66-67 0.35234565364413584 1103 68-69 0.38400642746617564 1103 70-71 0.23351069396144908 1103 72-73 0.37057650415736987 1103 74-75 0.36024630355998255 1103 76-77 0.06349536397826938 1103 78-79 0.40296235656624546 1103 80-81 0.33431801448355714 1103 82-83 0.36711048917247524 1103 84-85 0.49684867550532275 1103 86-87 0.5710424208570473 1103 88-89 0.5160307062304881 1103 90-91 0.29777954456888267 1103 92-93 0.46972547000717313 1103 94-95 0.5745213118322745 1103 96-97 0.40727415543624446 1103 98-99 0.3125439623714179 1103 100-101 0.9917966532445384 1104 1 0.05694943367453931 1104 2 -0.03075594167924578 1104 3 0.0929773558616489 1104 4 -0.004579843716960852 1104 5 0.07710551145084565 1104 6 0.07931196909463978 1104 7 -0.04027342116613397 1104 8 -0.04508191497148317 1104 9 -0.06753040021851575 1104 10-11 0.09721234797712697 1104 12-13 0.24411869648212559 1104 14-15 0.08308453066244681 1104 16-17 0.16081880136757576 1104 18-19 0.30098524169070373 1104 20-21 0.21355108876777962 1104 22-23 0.27810539475630236 1104 24-25 0.2498178943850533 1104 26-27 0.22479253307250957 1104 28-29 0.16870380759323922 1104 30-31 0.19370909025643357 1104 32-33 0.15406836387374057 1104 34-35 0.2547881223838644 1104 36-37 0.0791576656141757 1104 38-39 0.18864174250098387 1104 40-41 0.3384935644429916 1104 42-43 0.04015570122182055 1104 44-45 0.23192032594621992 1104 46-47 0.1056924382987603 1104 48-49 0.2136384944842149 1104 50-51 0.1915041905435828 1104 52-53 0.18552646969068576 1104 54-55 0.25050960847891446 1104 56-57 0.37107344961874844 1104 58-59 0.45029254493382354 1104 60-61 0.41819250403283803 1104 62-63 0.5369844273002187 1104 64-65 0.5366019123679706 1104 66-67 0.4170558025804354 1104 68-69 0.49778134494575 1104 70-71 0.4165099502019629 1104 72-73 0.07314831382869613 1104 74-75 0.18212881414779858 1104 76-77 0.19968325852423874 1104 78-79 0.4753799437121131 1104 80-81 0.6011887540017753 1104 82-83 0.3859306776325262 1104 84-85 0.2269703201358091 1104 86-87 0.06933853548206415 1104 88-89 0.3875164426445856 1104 90-91 0.6732963344783407 1104 92-93 0.6132071249642337 1104 94-95 0.8389038377870257 1104 96-97 0.9770430982528211 1104 98-99 1.100897672256039 1104 100-101 1.8933702270396218 1105 1 -0.10561005865756101 1105 2 -0.02441009292197549 1105 3 0.0423868393800646 1105 4 0.040881111685820315 1105 5 0.001783812173570709 1105 6 -5.223337433690745E-4 1105 7 0.14072428727737218 1105 8 0.14233215165992874 1105 9 -0.11043538876073455 1105 10-11 0.01253933546170316 1105 12-13 0.06542135821312911 1105 14-15 0.11849789219954943 1105 16-17 0.0487176203346138 1105 18-19 -0.08596169996274483 1105 20-21 0.09282131616069478 1105 22-23 0.018075428248288006 1105 24-25 -0.019581012718767 1105 26-27 0.07821224045837738 1105 28-29 0.010683359858347785 1105 30-31 0.049402726780314765 1105 32-33 0.1002344135828892 1105 34-35 0.3166865888037478 1105 36-37 0.27057314243420194 1105 38-39 0.248874423622091 1105 40-41 0.19280345339063842 1105 42-43 0.1380844866968829 1105 44-45 0.2626888787401498 1105 46-47 0.3891050947415522 1105 48-49 0.1657449637244781 1105 50-51 0.33205592683830787 1105 52-53 0.6250998876353364 1105 54-55 0.42465908847186284 1105 56-57 0.37332093772028685 1105 58-59 0.302055280699733 1105 60-61 0.28901041342822253 1105 62-63 0.24883001164712937 1105 64-65 0.20674998217230467 1105 66-67 0.24622907950622164 1105 68-69 0.0856096544821483 1105 70-71 0.2788050321691742 1105 72-73 0.23544339579591167 1105 74-75 0.307265426169657 1105 76-77 0.05617893982160993 1105 78-79 0.1454663177646438 1105 80-81 0.21296382891804555 1105 82-83 0.35983337461824405 1105 84-85 0.1918037416059306 1105 86-87 0.37745593368407526 1105 88-89 0.4151120710598839 1105 90-91 0.5602697171682749 1105 92-93 0.37202961747260943 1105 94-95 0.23805772461939156 1105 96-97 0.4022237786688265 1105 98-99 0.13920192044923496 1105 100-101 0.9843538335969946 1106 1 -0.00801360071954349 1106 2 -0.05583183724694152 1106 3 -0.10376538495052756 1106 4 -0.06769995514951432 1106 5 0.04005775974543013 1106 6 0.032199572223319706 1106 7 0.01666075505444553 1106 8 0.008577144280629057 1106 9 -0.14441528896454514 1106 10-11 0.008052020822582051 1106 12-13 0.029916403543090553 1106 14-15 -0.09993629150977057 1106 16-17 -0.1990404793210203 1106 18-19 -0.11881010814321513 1106 20-21 -0.11504993269726782 1106 22-23 -0.05976560063638914 1106 24-25 -0.11380759223946768 1106 26-27 -0.14510282033297273 1106 28-29 0.005422976134966007 1106 30-31 -0.2142876239119289 1106 32-33 -0.13494374370152684 1106 34-35 -0.3010799443327983 1106 36-37 -0.27628538592337293 1106 38-39 -0.10721525239168983 1106 40-41 -0.009761730388966328 1106 42-43 0.17829813488016555 1106 44-45 0.17159674296110694 1106 46-47 0.08588280224032019 1106 48-49 -0.12438786359999199 1106 50-51 -0.09701147073003824 1106 52-53 -0.04676039769743312 1106 54-55 -0.17956467988853575 1106 56-57 -0.331957015755016 1106 58-59 -0.06688842581513654 1106 60-61 -0.023667919553709282 1106 62-63 0.17348037358401314 1106 64-65 -0.200274404643352 1106 66-67 -0.14075876222544537 1106 68-69 0.0032672551568282415 1106 70-71 0.10483989055697407 1106 72-73 0.08141289470658464 1106 74-75 0.13428067671431876 1106 76-77 0.09784033556270444 1106 78-79 0.1052667253828865 1106 80-81 0.15727825719831756 1106 82-83 0.11201553780610496 1106 84-85 0.44184942296091023 1106 86-87 0.5559505573399903 1106 88-89 0.48275912988341574 1106 90-91 0.5393933379427516 1106 92-93 0.4973476299822721 1106 94-95 0.49200037694913945 1106 96-97 0.3544762208643455 1106 98-99 0.5692251125217638 1106 100-101 0.5817394545120287 1107 1 -0.08840575758228653 1107 2 -0.06482654624165463 1107 3 -0.005539427901041449 1107 4 0.009766587022909334 1107 5 -0.14839539929596413 1107 6 0.02799789155105259 1107 7 -0.0025424817958494828 1107 8 0.06877011035594904 1107 9 0.11257195037563861 1107 10-11 0.053305677840945975 1107 12-13 -0.011835852915048406 1107 14-15 0.06548543273547835 1107 16-17 0.08885245563074307 1107 18-19 0.16212671102889686 1107 20-21 0.09655915538240123 1107 22-23 0.09910461411617888 1107 24-25 0.09735020421244656 1107 26-27 0.01565036983198098 1107 28-29 0.1280498046441494 1107 30-31 0.039836247859000196 1107 32-33 0.049167678645183344 1107 34-35 0.029000976924585586 1107 36-37 0.14162622316470674 1107 38-39 -0.010265361324151456 1107 40-41 -0.053023479533067075 1107 42-43 -0.1094236204886414 1107 44-45 -0.2815934219937617 1107 46-47 -0.27722021674505015 1107 48-49 -0.39279744965664065 1107 50-51 -0.44402360891864845 1107 52-53 -0.4360538928732822 1107 54-55 -0.4019580816936994 1107 56-57 -0.31815371665759784 1107 58-59 -0.2848622820242781 1107 60-61 -0.40379552615190306 1107 62-63 -0.3825575971598383 1107 64-65 -0.5195087650541765 1107 66-67 -0.5726137264333531 1107 68-69 -0.2901967971307542 1107 70-71 -0.27410502074087617 1107 72-73 -0.2672021068495951 1107 74-75 -0.4906181717139404 1107 76-77 -0.5573728608269626 1107 78-79 -0.41630190206809203 1107 80-81 -0.19626982870271092 1107 82-83 -0.3156999928506039 1107 84-85 -0.2554739508330499 1107 86-87 -0.41574256435313117 1107 88-89 -0.09996729401948201 1107 90-91 0.03178362739186369 1107 92-93 0.0067625070442041135 1107 94-95 0.18115381610257586 1107 96-97 0.4049119507118384 1107 98-99 0.4974541819273064 1107 100-101 1.3549887542319183 1108 1 -0.04384132273708019 1108 2 -0.06827084212655166 1108 3 0.08876274221106684 1108 4 -0.07671335172484817 1108 5 -0.0438837602270894 1108 6 0.008130909481046444 1108 7 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-1.3791927558539285 2316 50-51 -1.486614891856462 2316 52-53 -1.3624892745066859 2316 54-55 -1.4636087705721081 2316 56-57 -1.4121895488122433 2316 58-59 -1.29594589038512 2316 60-61 -1.1479452982719778 2316 62-63 -1.243935877845047 2316 64-65 -1.169085746561855 2316 66-67 -1.2535739653143878 2316 68-69 -1.5141241898700244 2316 70-71 -1.4706174100750466 2316 72-73 -1.5146391124549154 2316 74-75 -1.718064606833643 2316 76-77 -1.7934590884973503 2316 78-79 -1.9092153600805624 2316 80-81 -1.8171064361729812 2316 82-83 -1.817415640605855 2316 84-85 -2.0748922021819958 2316 86-87 -2.063273865102257 2316 88-89 -2.410052945441766 2316 90-91 -2.8934298463364634 2316 92-93 -3.182207580440732 2316 94-95 -3.2607498980783625 2316 96-97 -3.40383392387562 2316 98-99 -3.860295045376379 2316 100-101 -3.898814483234684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 5.0 5 36.0 6 104.0 7 185.0 8 368.0 9 672.0 10 1114.0 11 1832.0 12 2678.0 13 3735.0 14 4386.0 15 6077.0 16 8002.0 17 11705.0 18 11256.0 19 6673.0 20 5590.0 21 5914.0 22 6357.0 23 6872.0 24 7827.0 25 9141.0 26 10871.0 27 12822.0 28 15589.0 29 18853.0 30 22745.0 31 27842.0 32 34794.0 33 44204.0 34 59403.0 35 86689.0 36 134671.0 37 212047.0 38 167750.0 39 13720.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.420057140413334 20.0313761159748 15.278228334259945 19.27033840935192 2 14.728637875272849 24.00104723682201 37.92077778112542 23.349537106779717 3 22.40266048021211 26.63129471924599 31.875270769937238 19.090774030604667 4 11.847905474409709 17.674095329718565 40.20745262759822 30.270546568273502 5 11.309869500307004 40.55578469680457 34.502992630886695 13.631353172001734 6 26.89216889187176 38.56875556474427 17.270057234401314 17.269018308982652 7 24.599052707803263 36.134137738654154 21.80475890177272 17.46205065176986 8 24.345554905649987 31.173476649631755 20.587553881269525 23.893414563448733 9 24.26358369011764 19.900200824283427 23.799911275769247 32.036304209829694 10-11 24.31521828342509 26.11635133548668 27.312206438636387 22.256223942451843 12-13 28.7757406759041 24.946988830512822 26.719447541019374 19.557822952563704 14-15 23.519526576808932 26.505589745268026 29.736063038591865 20.238820639331173 16-17 19.25961788706999 29.739646021652614 29.90551330808355 21.095222783193847 18-19 18.91805267975228 30.56586111852788 31.128958695442133 19.387127506277707 20-21 21.807667892944966 26.73638202534355 29.546779175363337 21.909170906348148 22-23 20.245279902091667 27.03465751304111 30.372101527947613 22.34796105691961 24-25 22.49782604856145 27.147173135882095 30.232054381512114 20.12294643404434 26-27 21.955662818833225 25.215135481069222 33.361557473873624 19.467644226223932 28-29 19.348739212058184 29.13816980820398 31.6815631256279 19.831527854109936 30-31 20.0749480797022 27.511316495122767 30.854059029664437 21.559676395510596 32-33 20.37073014639498 27.740035926040978 31.187086572616213 20.70214735494783 34-35 22.286128973775433 26.370331429117616 30.60736290971503 20.736176687391918 36-37 21.6223138151695 25.808933463969304 32.7031671603612 19.865585560500005 38-39 21.172442134061225 27.04727724996974 32.1089006497693 19.671379966199734 40-41 20.252391265161478 27.998152753182165 31.567505286938836 20.18195069471753 42-43 19.50670525948336 28.658594866850628 29.509427258220022 22.325272615445993 44-45 20.715979543441616 26.936892712156276 29.62710156333196 22.720026181070153 46-47 22.242879576283556 28.251520341433334 29.079857729183644 20.42574235309946 48-49 21.67502398627782 27.546999858186165 29.58241799342254 21.195558162113475 50-51 19.53446500721538 28.50294069672851 31.508882348235428 20.453711947820686 52-53 20.645785650985474 29.78266719167031 28.55231976462106 21.01922739272316 54-55 19.098410132431823 27.560561559967294 30.0586577291376 23.282370578463286 56-57 21.64949149795384 27.886732195155904 28.244486163071812 22.219290143818444 58-59 22.961845924003526 27.105658360271846 28.40644695783982 21.52604875788481 60-61 20.955117845590095 27.80164767204895 30.77450144647923 20.468733035881723 62-63 20.831077999403657 27.757541819345416 30.109357289568255 21.302022891682675 64-65 20.06557724494535 29.731675617046342 29.27158558339541 20.931161554612903 66-67 20.62588926862185 27.69074048966332 28.17914668653122 23.504223555183614 68-69 19.598645424411348 28.443645842586427 29.22945136987404 22.72825736312818 70-71 21.739098815313344 28.29388706672914 27.74393189636096 22.223082221596556 72-73 20.945255902914496 26.47452087357005 30.156576449846394 22.42364677366906 74-75 19.558290469002102 30.25272899734347 28.18090392744976 22.00807660620467 76-77 20.699820665493295 27.177707597375306 28.440174674306068 23.68229706282533 78-79 20.453013039552932 27.420566359802727 29.59145296836576 22.534967632278583 80-81 21.188208612068365 27.93582142118764 28.49528275913657 22.380687207607426 82-83 20.140815951245308 27.42773494519149 29.7729012927349 22.658547810828303 84-85 19.721713437357472 27.722270301381872 29.026537271968856 23.529478989291796 86-87 19.9263165506324 28.2403628613358 28.691494514058068 23.141826073973732 88-89 20.11862289751662 27.467910356651302 27.864067842947794 24.549398902884285 90-91 20.073521900207027 26.103766681330043 28.98480776728176 24.83790365118117 92-93 20.695809909894 27.969378712210386 28.87968516404113 22.455126213854488 94-95 19.92035597740545 27.294908330415684 28.843946129639193 23.940789562539674 96-97 17.96607475315004 27.220818511253697 29.97990707802786 24.833199657568404 98-99 19.648417249070942 27.822318819198927 27.514589110191544 25.014674821538584 100-101 19.36599378720663 26.116017163101407 29.092236086147967 25.425752963543996 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2037.0 1 1339.0 2 693.0 3 704.0 4 604.5 5 640.5 6 1004.0 7 1857.0 8 2800.0 9 3138.5 10 2692.0 11 2176.5 12 2078.0 13 2128.5 14 2193.5 15 2260.0 16 2319.0 17 2370.0 18 2422.0 19 2541.0 20 2765.5 21 3066.5 22 3494.0 23 4031.0 24 4523.5 25 5238.5 26 6610.5 27 8020.5 28 8793.0 29 9831.5 30 11508.0 31 13093.5 32 15174.0 33 17963.5 34 20983.0 35 22790.5 36 24522.5 37 26827.5 38 26851.0 39 25465.0 40 24386.5 41 24392.5 42 24824.0 43 25497.5 44 26912.0 45 27673.5 46 30113.5 47 48475.0 48 94099.5 49 90934.0 50 47261.5 51 31766.0 52 24256.5 53 19683.5 54 17162.0 55 15591.5 56 14103.5 57 12750.5 58 11685.0 59 10607.0 60 9609.0 61 8307.0 62 6729.5 63 5001.0 64 3464.0 65 2535.5 66 1737.0 67 1220.0 68 869.0 69 575.5 70 390.5 71 284.5 72 241.5 73 242.0 74 217.0 75 166.5 76 115.5 77 61.0 78 26.0 79 11.0 80 8.0 81 5.0 82 3.0 83 1.5 84 1.0 85 1.0 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3991551458495449 2 0.0 3 0.0 4 0.0 5 2.077850837321941E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011947642314601161 16-17 0.0017142269407906014 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0020259045663888926 36-37 0.006441337595698018 38-39 0.011895696043668113 40-41 0.0020259045663888926 42-43 5.194627093304853E-4 44-45 5.714089802635338E-4 46-47 2.597313546652427E-4 48-49 3.636238965313397E-4 50-51 6.233552511965823E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.5583881279914558E-4 60-61 0.002441474733853281 62-63 0.0 64-65 4.155701674643882E-4 66-67 0.0015064418570584074 68-69 0.0018181194826566987 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12477494278118258 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14487814963227236 88-89 0.4365564609213398 90-91 0.33526123260189516 92-93 0.0 94-95 0.0 96-97 5.194627093304853E-4 98-99 0.0 100-101 3.1167762559829117E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 962533.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.67102293224259 #Duplication Level Percentage of deduplicated Percentage of total 1 73.58991877453388 29.193873552864037 2 13.773402181619604 10.92809907604063 3 4.95506562652392 5.897175663017922 4 2.4170025338700847 3.835398517937943 5 1.3417065315725447 2.6613435291177048 6 0.8311626459199695 1.9783843432028725 7 0.5235509237417005 1.4538860491367658 8 0.37694426695562305 1.1963011726859116 9 0.2895053178479132 1.0336474893015672 >10 1.6680550844351574 12.35213688007447 >50 0.13880042356534372 3.8518517271084693 >100 0.07789513896962635 5.39122315780005 >500 0.004705075507753058 1.3135338283456552 >1k 0.00862597176421394 7.481553536550274 >5k 0.003136717005168705 7.871662817535224 >10k+ 5.227861675281176E-4 3.5599286592805193 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22168 2.3030898680876395 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12162 1.2635410941754723 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8694 0.9032417589838477 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7592 0.7887521778474089 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6993 0.7265205452696167 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6991 0.7263127601858845 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6181 0.6421598012743459 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6151 0.639043025018363 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5987 0.6220046481523231 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5897 0.6126543193843744 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5805 0.6030962055326934 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5308 0.5514616122252433 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5277 0.5482409434273942 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5034 0.5229950557539326 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4531 0.4707371071952858 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4072 0.42305043047874724 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3877 0.40279138481485827 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3738 0.38835032149547083 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3147 0.32694982925260746 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3073 0.31926178115451626 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3038 0.31562554218920286 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2978 0.309391989677237 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2805 0.29141857993440223 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2651 0.27541912848702327 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2550 0.26492598175854754 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2541 0.2639909488817526 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2253 0.23406989682431667 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2206 0.2291869473566101 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2115 0.2197327260467953 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2057 0.21370695861856168 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2030 0.21090185998817704 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2015 0.2093434718601856 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1976 0.2052916627274078 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1819 0.18898053365443054 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1722 0.17890295709341913 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1672 0.1737083300001143 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1648 0.17121490899532796 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1317 0.13682647763764982 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1291 0.1341252715491313 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1266 0.13152795800247888 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1251 0.12996956987448743 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1156 0.1200997783972082 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1119 0.11625575434816261 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1097 0.11397011842710847 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1094 0.11365844080151019 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1029 0.10690542558021386 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1014 0.10534703745222242 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 971 0.10087965815198026 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.194627093304853E-5 0.0 14-15 0.0 0.0 0.0 1.0389254186609706E-4 0.0 16-17 0.0 0.0 0.0 3.636238965313397E-4 0.0 18-19 0.0 0.0 0.0 6.233552511965823E-4 0.0 20-21 0.0 0.0 0.0 7.79194063995728E-4 0.0 22-23 0.0 0.0 0.0 0.0020259045663888926 0.0 24-25 0.0 0.0 0.0 0.004051809132777785 0.0 26-27 0.0 0.0 0.0 0.00670106895036326 0.0 28-29 0.0 0.0 0.0 0.017661732117236498 0.0 30-31 0.0 0.0 0.0 0.04633607367227929 0.0 32-33 0.0 0.0 0.0 0.10420421949169534 0.0 34-35 0.0 0.0 0.0 0.18648711264964424 0.0 36-37 0.0 0.0 0.0 0.29910662803249344 0.0 38-39 0.0 0.0 0.0 0.4588414111516177 0.0 40-41 0.0 0.0 0.0 0.6349392696146522 0.0 42-43 0.0 0.0 0.0 0.8159720238163263 0.0 44-45 0.0 0.0 0.0 1.0260427434695745 0.0 46-47 0.0 0.0 0.0 1.2840079249230936 0.0 48-49 0.0 0.0 0.0 1.5290904311852165 0.0 50-51 0.0 0.0 0.0 1.7949514458205589 0.0 52-53 0.0 0.0 0.0 2.047825892722639 0.0 54-55 0.0 0.0 0.0 2.318517910554755 0.0 56-57 0.0 0.0 0.0 2.6668176571608453 0.0 58-59 0.0 0.0 0.0 3.0210912249242363 0.0 60-61 0.0 0.0 0.0 3.442219643378461 0.0 62-63 0.0 0.0 0.0 3.8532704852716737 0.0 64-65 0.0 0.0 0.0 4.244477851668462 0.0 66-67 0.0 0.0 0.0 4.703163424007281 0.0 68-69 0.0 0.0 0.0 5.235197130903564 0.0 70-71 0.0 0.0 0.0 5.8141902667233225 0.0 72-73 0.0 0.0 0.0 6.392715886104684 0.0 74-75 0.0 0.0 0.0 7.018097041867655 0.0 76-77 0.0 0.0 0.0 7.617868686060634 0.0 78-79 0.0 0.0 0.0 8.394361543967843 0.0 80-81 0.0 0.0 0.0 9.20233384206048 0.0 82-83 0.0 0.0 0.0 9.947918668762526 0.0 84-85 0.0 0.0 0.0 10.804720461532227 0.0 86-87 0.0 0.0 0.0 11.67653472660158 0.0 88-89 0.0 0.0 0.0 12.542946579493897 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 120 0.0 33.611206 94-95 CGATATC 35 0.008110032 27.33432 84-85 CGGTGCA 70 0.008433151 27.114923 9 GTATAGG 300 0.0 26.989918 1 CTTTGCG 705 0.0 26.92122 2 TTGCGTT 725 0.0 26.178564 4 TTTGCGT 735 0.0 25.822393 3 GCTTTGC 810 0.0 23.520626 1 GAGTACT 5330 0.0 22.70293 12-13 CATATAG 210 3.3469405E-8 22.680605 1 AGTACTT 5415 0.0 22.478012 12-13 GTACATG 6385 0.0 20.961353 1 GTACTTT 5910 0.0 20.27416 14-15 TACATGG 6480 0.0 20.063162 2 ACATGGG 6520 0.0 19.721754 3 GTATTAC 125 0.005936507 19.051708 1 ATGGGAT 1535 0.0 18.855816 5 GGGAGCG 155 9.693127E-4 18.36722 7 GCGACGC 65 0.0076099862 18.25043 92-93 CATTGAC 130 0.007607293 18.25043 9 >>END_MODULE