FastQCFastQC Report
Thu 26 May 2016
SRR938907_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938907_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832957
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57970.31626492056278466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47420.2587076510796489No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29000.15821429526170008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52950.035.0497471
GTATCAA79550.032.3991621
ATCAACG96750.026.6358913
CAACGCA97650.026.2926585
TCAACGC97850.026.2880484
TATCAAC99400.025.9734382
AACGCAG100700.025.532196
GTACATG66900.023.2004171
CGCAGAG110950.022.7039838
ACGCAGA111700.022.636327
GTATTAC3150.022.6024151
TACATGG68200.021.6020662
GAGTACT54200.020.83677512-13
GCAGAGT117350.020.416689
ACATGGG69250.020.3852393
GTACTTT57700.019.53208214-15
AGTACTT57300.019.29628612-13
CATGGGG38700.018.7283614
AGAGTAC99600.018.61187610-11
GTGTAGC10200.017.6830651
Produced by FastQC (version 0.11.5)