##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938907_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1832957 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.822798898173826 33.0 31.0 34.0 30.0 34.0 2 31.920189617105038 33.0 31.0 34.0 30.0 34.0 3 31.961038911442003 34.0 31.0 34.0 30.0 34.0 4 35.50520170413163 37.0 35.0 37.0 33.0 37.0 5 35.535530838966764 37.0 35.0 37.0 33.0 37.0 6 35.61623322314708 37.0 35.0 37.0 33.0 37.0 7 35.54238588248388 37.0 35.0 37.0 33.0 37.0 8 35.52074925925704 37.0 35.0 37.0 33.0 37.0 9 37.138334941845336 39.0 37.0 39.0 33.0 39.0 10-11 37.01671779534381 39.0 37.0 39.0 33.0 39.0 12-13 36.89825429620008 39.0 37.0 39.0 33.0 39.0 14-15 38.114381297542714 40.0 38.0 41.0 33.0 41.0 16-17 38.017278092175644 40.0 38.0 41.0 33.0 41.0 18-19 37.913408770636735 40.0 38.0 41.0 33.0 41.0 20-21 38.03933698390088 40.0 38.0 41.0 33.0 41.0 22-23 37.97980885530866 40.0 38.0 41.0 33.0 41.0 24-25 37.88968562819531 40.0 38.0 41.0 32.5 41.0 26-27 37.77832731482517 40.0 38.0 41.0 32.0 41.0 28-29 37.6357634139808 40.0 37.5 41.0 32.0 41.0 30-31 37.44305049163728 40.0 37.0 41.0 31.5 41.0 32-33 37.24730067317455 40.0 37.0 41.0 31.0 41.0 34-35 36.935455114331646 40.0 36.0 41.0 30.5 41.0 36-37 36.670323144514576 39.5 36.0 41.0 30.0 41.0 38-39 36.43565642838321 39.0 35.5 41.0 30.0 41.0 40-41 36.278350774186194 39.0 35.0 41.0 30.0 41.0 42-43 36.06784419929109 39.0 35.0 41.0 29.0 41.0 44-45 36.01162929626827 39.0 35.0 41.0 28.5 41.0 46-47 35.80031310063465 39.0 35.0 41.0 27.5 41.0 48-49 35.56380673414597 39.0 35.0 41.0 27.0 41.0 50-51 34.47512407546931 38.0 34.0 40.0 25.0 40.5 52-53 34.57358192254374 38.0 34.0 39.5 25.0 40.5 54-55 35.043273792020216 39.0 35.0 40.0 26.0 41.0 56-57 34.94110800198804 38.5 34.5 40.0 26.0 41.0 58-59 34.7110646894608 38.0 34.0 40.0 25.0 41.0 60-61 34.39169385861207 38.0 34.0 40.0 24.5 41.0 62-63 34.0034136098119 37.5 34.0 40.0 23.0 41.0 64-65 33.60112048455038 37.0 33.0 40.0 22.0 41.0 66-67 33.18274269390935 36.0 33.0 39.0 21.5 41.0 68-69 32.739113356178024 36.0 32.0 39.0 20.0 40.5 70-71 32.294521639078276 35.0 32.0 38.5 18.5 40.0 72-73 31.847464506805125 35.0 31.5 37.5 17.5 40.0 74-75 31.363645191894847 35.0 31.0 37.0 13.5 39.0 76-77 30.920732455807745 35.0 31.0 36.5 9.5 39.0 78-79 30.514220737311348 35.0 30.5 36.0 7.0 38.5 80-81 30.05855101892734 34.0 30.0 35.5 3.5 37.0 82-83 29.583127154646835 34.0 29.5 35.0 2.0 37.0 84-85 29.16556989607503 34.0 29.0 35.0 2.0 36.5 86-87 28.702978302273323 34.0 29.0 35.0 2.0 36.0 88-89 28.22485170137652 34.0 28.5 35.0 2.0 36.0 90-91 27.758594718806826 34.0 27.0 35.0 2.0 35.0 92-93 27.234730547416007 33.0 26.0 35.0 2.0 35.0 94-95 26.684562703871393 33.0 25.0 35.0 2.0 35.0 96-97 26.007753318817628 33.0 24.5 35.0 2.0 35.0 98-99 25.095087882585354 32.0 18.5 35.0 2.0 35.0 100-101 21.778858696630635 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1578768145622682 1101 2 0.25505559207407913 1101 3 0.25695241838952043 1101 4 0.25524667292987857 1101 5 0.15884500267291912 1101 6 0.1994391221294265 1101 7 0.2739775624663281 1101 8 0.345091019821993 1101 9 0.28869509371348556 1101 10-11 0.21296241908233782 1101 12-13 0.3068545712493247 1101 14-15 0.4075223520263336 1101 16-17 0.31860490465871294 1101 18-19 0.4598406975385032 1101 20-21 0.3325468628341426 1101 22-23 0.3968655882140766 1101 24-25 0.44361023171951075 1101 26-27 0.39280906936117077 1101 28-29 0.5683087437549688 1101 30-31 0.486746144918655 1101 32-33 0.5198339791901532 1101 34-35 0.3537010200566968 1101 36-37 0.2963173466173288 1101 38-39 0.474653948353442 1101 40-41 0.419324129587757 1101 42-43 0.3430046054157927 1101 44-45 0.5009515602131032 1101 46-47 0.3578846163626821 1101 48-49 0.2121251623603726 1101 50-51 0.25640479230091984 1101 52-53 0.28369273086047997 1101 54-55 0.4827118078778696 1101 56-57 0.42920112946666933 1101 58-59 0.4406482473567124 1101 60-61 0.531191058425307 1101 62-63 0.4654673894154655 1101 64-65 0.4514089390769058 1101 66-67 0.464371159309934 1101 68-69 0.5510286449902324 1101 70-71 0.4435261872608862 1101 72-73 0.45033756075294207 1101 74-75 0.20670192629062356 1101 76-77 0.0061451447121179115 1101 78-79 -0.011713653869847462 1101 80-81 -0.09416560527629159 1101 82-83 0.1005439930248464 1101 84-85 0.08956668441312132 1101 86-87 -0.007699719319052889 1101 88-89 -0.1457327631368983 1101 90-91 0.06579893904631007 1101 92-93 0.018723159371422327 1101 94-95 -0.060074254720529296 1101 96-97 -0.24748881364980235 1101 98-99 -0.2855496323650826 1101 100-101 0.3013655223958196 1102 1 0.24063645316798343 1102 2 0.21800252631356898 1102 3 0.20558035966027788 1102 4 0.11515295832664663 1102 5 0.19446850665872972 1102 6 0.20705677295971725 1102 7 0.2601982031878336 1102 8 0.34939005703000703 1102 9 0.3426945273652535 1102 10-11 0.4116404501139428 1102 12-13 0.43714872503958446 1102 14-15 0.5584407978813104 1102 16-17 0.5590016957082753 1102 18-19 0.5420404995837629 1102 20-21 0.5006666334631049 1102 22-23 0.5193153515232538 1102 24-25 0.6098811892754483 1102 26-27 0.586366925038547 1102 28-29 0.5053250436310179 1102 30-31 0.46927817565105556 1102 32-33 0.570462371936614 1102 34-35 0.48193581681248787 1102 36-37 0.6044712250341178 1102 38-39 0.6139754458102189 1102 40-41 0.4530407163779486 1102 42-43 0.37816528001133776 1102 44-45 0.549716279924148 1102 46-47 0.6093771242701891 1102 48-49 0.7240855954564864 1102 50-51 0.7083566901017804 1102 52-53 0.6052098896089575 1102 54-55 0.705442107240998 1102 56-57 0.5603857122389897 1102 58-59 0.5929214542768477 1102 60-61 0.6393819364787703 1102 62-63 0.5908919836216171 1102 64-65 0.4401721360592319 1102 66-67 0.4172149887856875 1102 68-69 0.49648824192126995 1102 70-71 0.3709282584391005 1102 72-73 0.2546310213009093 1102 74-75 0.35347627926448766 1102 76-77 0.34548557609849695 1102 78-79 0.40743395865179366 1102 80-81 0.3596048847752158 1102 82-83 0.42770175974280633 1102 84-85 0.11340056138732635 1102 86-87 0.1750865002530837 1102 88-89 0.0867465063807451 1102 90-91 0.02789351252197747 1102 92-93 0.1397667361276369 1102 94-95 0.2228641041519026 1102 96-97 -0.03190064632061507 1102 98-99 -0.3544900068494954 1102 100-101 -0.00467740653281723 1103 1 0.2558558573547778 1103 2 0.23508787252935548 1103 3 0.28707794420133936 1103 4 0.2977213963298695 1103 5 0.11170985126420874 1103 6 0.262431169094981 1103 7 0.2981531146210017 1103 8 0.2767350650815388 1103 9 0.42996908453111615 1103 10-11 0.30838561919606633 1103 12-13 0.3084993691619573 1103 14-15 0.4570639862871033 1103 16-17 0.4496478309739729 1103 18-19 0.3655021541731429 1103 20-21 0.4549918146225238 1103 22-23 0.5095317363863785 1103 24-25 0.5047151264735206 1103 26-27 0.4887793646520677 1103 28-29 0.3862001442751364 1103 30-31 0.47353139626297036 1103 32-33 0.4191831851411223 1103 34-35 0.42474076850330533 1103 36-37 0.3803659512821085 1103 38-39 0.5152893548279351 1103 40-41 0.4723789812732804 1103 42-43 0.48741749418202573 1103 44-45 0.5951108048838947 1103 46-47 0.5612043183120576 1103 48-49 0.5296139770989967 1103 50-51 0.41922021667182463 1103 52-53 0.4847539717345626 1103 54-55 0.5395841160976786 1103 56-57 0.5010846856689497 1103 58-59 0.5106801096713696 1103 60-61 0.6094091103918231 1103 62-63 0.5190174868422304 1103 64-65 0.4841234243039949 1103 66-67 0.473717605532876 1103 68-69 0.46019194562497745 1103 70-71 0.3968486489382954 1103 72-73 0.19608281276629214 1103 74-75 0.1017753935962098 1103 76-77 0.1573384343915727 1103 78-79 0.14942218328947376 1103 80-81 0.1346370908943797 1103 82-83 0.33308068486358167 1103 84-85 0.27402807749038516 1103 86-87 0.41645526031749824 1103 88-89 0.4729099524354936 1103 90-91 0.49316072266690725 1103 92-93 0.24084564111958784 1103 94-95 0.2677332667928063 1103 96-97 0.34147244144105926 1103 98-99 0.24111335466419348 1103 100-101 0.4355988615831201 1104 1 0.1178358532723145 1104 2 0.17270779496250555 1104 3 0.1435302814130175 1104 4 0.2299442989630549 1104 5 0.24398174348710455 1104 6 0.15382124870306768 1104 7 0.1916170868602336 1104 8 0.06779446495906427 1104 9 0.04339223211225374 1104 10-11 0.18729968329078162 1104 12-13 0.2048006551386905 1104 14-15 0.3717976053930414 1104 16-17 0.27775560807133814 1104 18-19 0.33074404027234294 1104 20-21 0.31964990811098914 1104 22-23 0.4728752758842347 1104 24-25 0.4260011032034683 1104 26-27 0.334143724725557 1104 28-29 0.26899663335454704 1104 30-31 0.45945097168653604 1104 32-33 0.5308304600952027 1104 34-35 0.6413890640738273 1104 36-37 0.44847781084528293 1104 38-39 0.5919049906719849 1104 40-41 0.5865900447556172 1104 42-43 0.48020785956271794 1104 44-45 0.6963151322976486 1104 46-47 0.6916513483181816 1104 48-49 0.6915572308242446 1104 50-51 0.48042046161977936 1104 52-53 0.5262359068233522 1104 54-55 0.6705006296718778 1104 56-57 0.793781154336024 1104 58-59 0.7657321611104422 1104 60-61 0.7432405045538957 1104 62-63 0.5798543923331465 1104 64-65 0.44667904790064483 1104 66-67 0.536191579813341 1104 68-69 0.5745581428080726 1104 70-71 0.5790041908856338 1104 72-73 0.4462359052341398 1104 74-75 0.5541615948617746 1104 76-77 0.6647399449039213 1104 78-79 0.478758782595456 1104 80-81 0.5210628920782945 1104 82-83 0.7931296744532936 1104 84-85 0.5973675345224336 1104 86-87 0.569833500774731 1104 88-89 0.4122485929743789 1104 90-91 0.457463641629964 1104 92-93 0.5128235958042175 1104 94-95 0.6136120175582676 1104 96-97 0.6308743601999893 1104 98-99 0.504187451418634 1104 100-101 0.5024778164718775 1105 1 0.285416783042276 1105 2 0.2172010414574146 1105 3 0.29245709972608225 1105 4 0.24213557876029057 1105 5 0.17572193406107317 1105 6 0.2101730726306883 1105 7 0.19808312683585427 1105 8 0.28508407964031335 1105 9 0.30428125646337634 1105 10-11 0.2684567624399321 1105 12-13 0.18995128557277496 1105 14-15 0.2530117925661983 1105 16-17 0.3756857630329975 1105 18-19 0.42260277812084723 1105 20-21 0.36059331341754586 1105 22-23 0.23221372113432182 1105 24-25 0.3397636557396382 1105 26-27 0.24790435227215823 1105 28-29 0.3636645726532066 1105 30-31 0.33717290694271185 1105 32-33 0.4285270496553366 1105 34-35 0.46200810863734176 1105 36-37 0.4540886742030992 1105 38-39 0.38078224050354237 1105 40-41 0.47182617680990546 1105 42-43 0.5423866269277298 1105 44-45 0.6958712617852854 1105 46-47 0.6856388038027958 1105 48-49 0.4076427645276084 1105 50-51 0.44047355924883647 1105 52-53 0.3764742846988085 1105 54-55 0.5213003024236471 1105 56-57 0.2785405306393969 1105 58-59 0.3289231521592342 1105 60-61 0.5723142637376952 1105 62-63 0.44634722174698993 1105 64-65 0.48718291392137303 1105 66-67 0.40903799772857496 1105 68-69 0.45645618252702747 1105 70-71 0.33923599171338026 1105 72-73 0.27472944047749337 1105 74-75 0.34287506016383773 1105 76-77 0.2960373878761047 1105 78-79 0.4510878595629535 1105 80-81 0.5362268370235945 1105 82-83 0.4706398608564477 1105 84-85 0.5316220823102995 1105 86-87 0.4896060311735262 1105 88-89 0.4323815776707107 1105 90-91 0.5893198128256998 1105 92-93 0.4286598433578739 1105 94-95 0.3748799440236681 1105 96-97 0.3376095778842263 1105 98-99 0.5623394614285786 1105 100-101 0.7358609856463758 1106 1 0.21352732802612806 1106 2 0.3001494364549089 1106 3 0.33147017705427473 1106 4 0.27052023367999567 1106 5 0.29137316723853246 1106 6 0.36399347986160535 1106 7 0.2918344355204425 1106 8 0.42146075237716474 1106 9 0.46381672895402914 1106 10-11 0.4256009112714807 1106 12-13 0.37078833609910333 1106 14-15 0.5049821163585619 1106 16-17 0.5675574833901038 1106 18-19 0.606463762505463 1106 20-21 0.5754794816525148 1106 22-23 0.7058039902316011 1106 24-25 0.6068296959297399 1106 26-27 0.607013590157699 1106 28-29 0.5716872057583231 1106 30-31 0.5356540396766434 1106 32-33 0.6223828944786547 1106 34-35 0.6168137570786527 1106 36-37 0.5142144031007589 1106 38-39 0.6591277141997764 1106 40-41 0.8019692279326804 1106 42-43 0.8638988635252076 1106 44-45 0.7464716704121273 1106 46-47 0.7309318222481096 1106 48-49 0.7791565874094388 1106 50-51 0.8150322549423876 1106 52-53 0.7266941437098566 1106 54-55 0.7232763368396817 1106 56-57 0.7940377356063735 1106 58-59 0.8451115012179429 1106 60-61 0.9916770850395906 1106 62-63 0.9421259604605723 1106 64-65 0.7303912374726167 1106 66-67 0.7247748902609885 1106 68-69 0.8213618620595113 1106 70-71 0.7927018643918515 1106 72-73 0.6647905758763812 1106 74-75 0.8385886025906011 1106 76-77 0.7008441467164701 1106 78-79 0.7180580881782532 1106 80-81 0.8440343699707178 1106 82-83 0.8487473919000372 1106 84-85 0.8431080500389321 1106 86-87 0.8484022951566246 1106 88-89 0.9122172068862646 1106 90-91 1.0246887191143657 1106 92-93 1.012525427967109 1106 94-95 0.9179888075461058 1106 96-97 0.9488617272995228 1106 98-99 1.0293802796974987 1106 100-101 1.1781583060948506 1107 1 0.19610131731070268 1107 2 0.23651076818474337 1107 3 0.18705156449441418 1107 4 0.22690635989691543 1107 5 0.25757290198080085 1107 6 0.28853260228515865 1107 7 0.1481108105787996 1107 8 0.25734598048953927 1107 9 0.179986939088856 1107 10-11 0.24062290787691865 1107 12-13 0.3824114475289946 1107 14-15 0.3795192292538587 1107 16-17 0.22798446177067433 1107 18-19 0.26496313955170336 1107 20-21 0.21143071503913546 1107 22-23 0.2498700078721683 1107 24-25 0.0953506522178671 1107 26-27 0.13123412506320875 1107 28-29 0.08186840337533141 1107 30-31 0.27534114066935444 1107 32-33 0.40051938163777123 1107 34-35 0.18947335226692275 1107 36-37 0.11437470162391605 1107 38-39 0.3872420965480856 1107 40-41 0.4418439879486371 1107 42-43 0.20473850626200374 1107 44-45 0.15874123162391385 1107 46-47 0.4225955261487542 1107 48-49 0.30174043884038326 1107 50-51 0.34168462819526013 1107 52-53 0.37013736217031123 1107 54-55 0.5120038108008274 1107 56-57 0.422996892717471 1107 58-59 0.4389458047927377 1107 60-61 0.32788198272368874 1107 62-63 0.394310317674325 1107 64-65 0.3970012251372097 1107 66-67 0.28845823189006126 1107 68-69 0.3394816237603777 1107 70-71 0.21300412835730498 1107 72-73 0.26076842778341813 1107 74-75 0.27626911233062756 1107 76-77 0.2689932784456843 1107 78-79 0.23636535432326866 1107 80-81 0.3139406022907316 1107 82-83 0.21384312273454498 1107 84-85 0.3424789131158583 1107 86-87 0.336810099546053 1107 88-89 0.23995333418524112 1107 90-91 0.2017079085634137 1107 92-93 0.3680830102263748 1107 94-95 0.45676188234075 1107 96-97 0.6677710790257052 1107 98-99 0.4906322467165012 1107 100-101 0.7316774538075421 1108 1 0.19623735045702873 1108 2 0.11042329760032032 1108 3 0.20529399081720712 1108 4 0.18927476054123105 1108 5 0.07794621761711085 1108 6 0.14398403527357573 1108 7 0.1761012196063092 1108 8 0.1391833941815932 1108 9 0.22912541701779077 1108 10-11 0.2204534512594165 1108 12-13 0.14620991251571525 1108 14-15 0.19690013350559354 1108 16-17 0.3037396682168705 1108 18-19 0.3431125929400096 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-1.2853900332529378 2316 66-67 -1.4713134503677452 2316 68-69 -1.6567128162797893 2316 70-71 -1.4571058024074546 2316 72-73 -1.4589585951012047 2316 74-75 -1.4363716043333952 2316 76-77 -1.5625269900390677 2316 78-79 -1.5250809223005888 2316 80-81 -1.410342205171876 2316 82-83 -1.2592278037492548 2316 84-85 -1.40504024549098 2316 86-87 -1.25361720345062 2316 88-89 -1.295092117957882 2316 90-91 -1.4052761303351637 2316 92-93 -1.423936967576065 2316 94-95 -1.390237747699949 2316 96-97 -1.621426109283579 2316 98-99 -1.632851448648438 2316 100-101 -1.4918649065328182 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5438.0 3 1583.0 4 1661.0 5 1850.0 6 2151.0 7 2636.0 8 3190.0 9 3993.0 10 4930.0 11 6210.0 12 8018.0 13 11235.0 14 9967.0 15 10106.0 16 12496.0 17 15980.0 18 14481.0 19 11289.0 20 11458.0 21 12221.0 22 13292.0 23 14739.0 24 16770.0 25 19317.0 26 22151.0 27 25811.0 28 30713.0 29 36432.0 30 44187.0 31 54197.0 32 67217.0 33 86800.0 34 116806.0 35 166159.0 36 252111.0 37 371394.0 38 304236.0 39 39722.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27362456589289 18.865996758083174 14.500080445944155 23.360298230079778 2 15.32655606372337 19.973124620512216 40.64587182813735 24.05444748762706 3 19.20083790081382 24.1991368704208 29.726693835826268 26.873331392939114 4 13.07539903580455 16.54597840499267 36.630798770618036 33.747823788584746 5 14.180507386875666 36.81968377072012 33.998033807586225 15.00177503481799 6 32.39687402941146 35.402157306167496 17.45681102746878 14.74415763695226 7 28.79370210433865 32.47857369388371 20.665543290117725 18.062180911659915 8 26.101137205388586 35.24719874537855 19.755593365965282 18.89607068326758 9 26.559266511381878 17.089933255199647 20.406648168598444 35.94415206482003 10-11 25.13465226227264 26.410785063492764 27.100463388285977 21.354099285948617 12-13 26.077667438601182 23.772426244317955 27.62719520299785 22.522711114083005 14-15 23.05026256686988 24.97517070735714 25.951148179089685 26.023418546683303 16-17 22.576941258156467 28.27972654753158 26.54635798823351 22.596974206078443 18-19 22.509377883888654 26.89260204172152 28.627172785344108 21.970847289045718 20-21 23.17679260928927 26.169074026661022 28.62224473786353 22.031888626186188 22-23 23.015890565330018 25.982762767337213 28.52638886500213 22.47495780233064 24-25 22.83053710880977 26.223846482304776 28.554142791463132 22.39147361742232 26-27 22.640162970865845 26.449350338767335 28.458558127167287 22.451928563199534 28-29 22.45492978278828 26.470723127263472 28.624576924987892 22.449770164960352 30-31 22.719310338238365 26.282817556565284 28.448301983623807 22.54957012157254 32-33 22.389759802244996 26.42074309346495 28.489625969319697 22.699871134970355 34-35 22.853953213478043 26.412016357086866 28.2887581282655 22.445272301169595 36-37 22.91546866014951 26.224186785410243 28.24870587887037 22.611638675569875 38-39 22.698987746695813 26.38878955702923 28.448836419745476 22.463386276529484 40-41 22.84450401263352 26.305160697123153 28.45654706392268 22.39378822632064 42-43 22.680648847744408 26.348217148588766 28.172149095762318 22.79898490790451 44-45 22.692892007664344 26.534027045568102 28.03532220590843 22.737758740859125 46-47 22.817032803499654 26.606095453376955 27.694831586557623 22.88204015656577 48-49 22.94248542015927 26.67872379383957 27.545236155592857 22.8335546304083 50-51 22.805783614153086 26.812792892703385 27.572241042214923 22.80918245092861 52-53 23.011264276324518 26.76847164798972 27.422384048890073 22.79788002679569 54-55 22.81016506360976 26.668601626791688 27.47470711117031 23.04652619842824 56-57 22.799295436945826 27.115237381739345 27.31442331985332 22.771043861461507 58-59 22.97363087943553 26.715285991272854 27.44528819127948 22.86579493801214 60-61 22.938522728112993 26.561935583345452 27.752778556008067 22.746763132533488 62-63 22.798280603624875 26.64883507743998 27.74580667615797 22.80707764277718 64-65 22.74008995809649 26.555647626751473 27.7025599051353 23.001702510016738 66-67 22.628855183485133 26.88167385635679 27.397434519723586 23.092036440434498 68-69 22.553172238984796 26.783125807950714 27.663848635103065 22.99985331796142 70-71 22.628515687436206 26.623610373538064 27.626167486366587 23.121706452659144 72-73 22.71736785417734 26.6175203535859 27.449372690955276 23.215739101281482 74-75 22.67008312330002 26.878745030612688 27.25071919391069 23.200452652176608 76-77 22.784096499529554 26.70460914505817 27.371562490664438 23.139731864747837 78-79 22.71824499734474 26.778434148215496 27.318501151708162 23.184819702731602 80-81 22.65080546044221 26.944457004429793 27.31752967467811 23.087207860449883 82-83 22.78283866370687 26.81054564389268 27.293156841787937 23.113458850612513 84-85 22.825412825355272 26.679509178175188 27.101399110232656 23.39367888623689 86-87 22.71374029169518 26.550471674455967 27.455424496047886 23.280363537800966 88-89 22.802492361428587 26.569566089676332 27.36135184350768 23.2665897053874 90-91 22.78332887442546 26.622859724220206 27.400984833865476 23.19282656748886 92-93 22.75512562162494 26.550488377008246 27.337050724266216 23.3573352771006 94-95 22.76973798197653 26.484008500727036 27.277881222297523 23.468372294998915 96-97 22.774472130496832 26.398683540361944 27.27322568247621 23.55361864666502 98-99 22.735854306491145 26.32377681593979 27.166869935581712 23.773498941987352 100-101 23.09174652639311 25.73167405994379 27.681361404489685 23.495218009173417 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1331.0 1 789.0 2 272.5 3 309.0 4 290.0 5 290.0 6 483.5 7 997.0 8 1655.5 9 2075.5 10 2037.0 11 1931.5 12 2035.5 13 2162.5 14 2355.5 15 2579.0 16 2690.0 17 2774.5 18 2895.0 19 3025.5 20 3258.0 21 3607.5 22 4127.0 23 4994.5 24 6003.0 25 7256.0 26 9115.0 27 11238.5 28 13307.0 29 15602.0 30 18254.0 31 21611.0 32 24679.5 33 27539.0 34 31016.5 35 34412.0 36 39051.0 37 43729.5 38 46492.0 39 48067.0 40 49467.5 41 52476.0 42 55320.0 43 58270.0 44 62461.0 45 65428.0 46 66817.0 47 68778.5 48 74137.5 49 75173.0 50 71694.5 51 70239.0 52 68120.5 53 66730.5 54 65753.5 55 61833.5 56 56532.0 57 51409.0 58 46296.5 59 41326.5 60 35476.0 61 28834.5 62 23338.5 63 18697.0 64 14443.5 65 11033.5 66 7918.0 67 6000.0 68 4493.0 69 2887.0 70 1909.5 71 1241.5 72 823.0 73 575.0 74 417.5 75 292.0 76 193.0 77 128.0 78 79.5 79 49.0 80 30.0 81 23.5 82 19.0 83 18.5 84 15.5 85 10.5 86 13.0 87 13.5 88 9.5 89 5.5 90 2.5 91 4.5 92 7.0 93 4.0 94 4.0 95 4.5 96 2.0 97 1.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30786319591785294 2 0.08407180310285511 3 0.0935101041650186 4 0.09782007979456146 5 0.10949520365180415 6 0.05182882086159141 7 0.05346552046774693 8 0.05510222007390245 9 0.0575027128295972 10-11 0.05542955999513355 12-13 0.053438242140977664 14-15 0.05169242922774511 16-17 0.05319273720005434 18-19 0.0611307302899086 20-21 0.06241281164806376 22-23 0.07629747997361641 24-25 0.08505382286654842 26-27 0.07987094078038928 28-29 0.07779778794592562 30-31 0.0728058541471513 32-33 0.0652224793052974 34-35 0.3063083312920052 36-37 0.477152491847872 38-39 0.4509380198226145 40-41 0.4348710853555211 42-43 0.4632951018490887 44-45 0.4727606812380214 46-47 0.4663502744472456 48-49 0.4835356203118786 50-51 0.4802894994263368 52-53 0.4791710880287972 54-55 0.4708239200374041 56-57 0.45172909129892297 58-59 0.43440735380044376 60-61 0.3946355533708647 62-63 0.4626131436798572 64-65 0.4847631450164952 66-67 0.4936831578700428 68-69 0.5065312497783636 70-71 0.5030396239518985 72-73 0.4918555099765024 74-75 0.4931921479881961 76-77 0.4700874052146341 78-79 0.4515108646847689 80-81 0.4571574783260055 82-83 0.4641134516521664 84-85 0.4663229961204764 86-87 0.466595779388169 88-89 0.47262428960417513 90-91 0.4851723199180341 92-93 0.4914190567481943 94-95 0.4971747836965079 96-97 0.489591408854654 98-99 0.492019179937118 100-101 0.4727061245844829 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1832957.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.11802205011869 #Duplication Level Percentage of deduplicated Percentage of total 1 78.68915503059935 46.5194720220418 2 12.048496965801444 14.24566618590076 3 3.899759607344383 6.9163822337144225 4 1.7373976526359831 4.108460509534339 5 0.9700427301915315 2.8673503756510144 6 0.5797277298489272 2.0563414029764475 7 0.38983246697402374 1.6132287064895752 8 0.2690427680245195 1.2724221033998808 9 0.21088219132944247 1.1220244233292198 >10 1.0994929475383814 11.734495453049982 >50 0.07279287078889099 2.97187442008551 >100 0.03208296075340297 3.3875186260117243 >500 9.243415497894062E-4 0.38273528983252614 >1k 2.773024649368219E-4 0.4852497678059441 >5k 9.243415497894061E-5 0.3167784801769209 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5797 0.31626492056278466 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4742 0.2587076510796489 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2900 0.15821429526170008 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 5.455665353851727E-5 10-11 0.0 0.0 0.0 0.0 5.455665353851727E-5 12-13 0.0 0.0 0.0 0.0 1.0911330707703455E-4 14-15 0.0 0.0 0.0 2.7278326769258637E-5 1.363916338462932E-4 16-17 0.0 0.0 0.0 8.18349803077759E-5 1.636699606155518E-4 18-19 0.0 0.0 0.0 1.0911330707703455E-4 1.636699606155518E-4 20-21 0.0 0.0 0.0 1.363916338462932E-4 2.182266141540691E-4 22-23 0.0 0.0 0.0 2.727832676925863E-4 2.182266141540691E-4 24-25 0.0 0.0 0.0 3.818965747696209E-4 2.182266141540691E-4 26-27 0.0 0.0 0.0 7.365148227699832E-4 2.182266141540691E-4 28-29 0.0 0.0 0.0 0.002536884389541053 2.182266141540691E-4 30-31 0.0 0.0 0.0 0.0068468600190839165 2.182266141540691E-4 32-33 0.0 0.0 0.0 0.015875986179708527 2.182266141540691E-4 34-35 0.0 0.0 0.0 0.03164285905234002 2.182266141540691E-4 36-37 0.0 0.0 0.0 0.05714809458159684 2.182266141540691E-4 38-39 0.0 0.0 0.0 0.09162789961793975 3.273399212311036E-4 40-41 0.0 0.0 0.0 0.1293811038665937 3.273399212311036E-4 42-43 0.0 0.0 0.0 0.17043498565432796 3.5461824800036223E-4 44-45 2.7278326769258637E-5 0.0 0.0 0.22441879433069079 4.0917490153887957E-4 46-47 5.455665353851727E-5 0.0 0.0 0.28920482040768003 4.364532283081382E-4 48-49 5.455665353851727E-5 0.0 0.0 0.35764614227174996 5.455665353851727E-4 50-51 5.455665353851727E-5 0.0 0.0 0.434434632127213 5.455665353851727E-4 52-53 5.455665353851727E-5 0.0 0.0 0.5108139470811373 5.455665353851727E-4 54-55 5.455665353851727E-5 0.0 0.0 0.6007233121126136 5.455665353851727E-4 56-57 5.455665353851727E-5 0.0 0.0 0.7096456709022634 5.455665353851727E-4 58-59 5.455665353851727E-5 0.0 0.0 0.830679606777464 5.728448621544313E-4 60-61 5.455665353851727E-5 0.0 0.0 0.9695262900329904 6.0012318892369E-4 62-63 5.455665353851727E-5 0.0 0.0 1.1100915078749802 6.0012318892369E-4 64-65 5.455665353851727E-5 0.0 0.0 1.258049152271439 6.0012318892369E-4 66-67 5.455665353851727E-5 0.0 0.0 1.4314301972168468 6.0012318892369E-4 68-69 5.455665353851727E-5 0.0 0.0 1.6323077955456675 6.0012318892369E-4 70-71 5.455665353851727E-5 0.0 0.0 1.8558809617465113 6.0012318892369E-4 72-73 5.455665353851727E-5 0.0 0.0 2.0893834388913652 6.274015156929486E-4 74-75 5.455665353851727E-5 0.0 0.0 2.3354612246768474 6.546798424622072E-4 76-77 5.455665353851727E-5 0.0 0.0 2.6070442459915864 6.546798424622072E-4 78-79 5.455665353851727E-5 0.0 0.0 2.9052781925598907 7.092364960007246E-4 80-81 5.455665353851727E-5 0.0 0.0 3.237473655955923 7.365148227699832E-4 82-83 5.455665353851727E-5 0.0 0.0 3.590646152637514 8.456281298470176E-4 84-85 5.455665353851727E-5 0.0 0.0 3.9542389701449623 8.729064566162764E-4 86-87 5.455665353851727E-5 0.0 0.0 4.345273784382284 9.547414369240522E-4 88-89 5.455665353851727E-5 0.0 0.0 4.765469129935945 0.001036576417231828 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5295 0.0 35.049747 1 GTATCAA 7955 0.0 32.399162 1 ATCAACG 9675 0.0 26.635891 3 CAACGCA 9765 0.0 26.292658 5 TCAACGC 9785 0.0 26.288048 4 TATCAAC 9940 0.0 25.973438 2 AACGCAG 10070 0.0 25.53219 6 GTACATG 6690 0.0 23.200417 1 CGCAGAG 11095 0.0 22.703983 8 ACGCAGA 11170 0.0 22.63632 7 GTATTAC 315 0.0 22.602415 1 TACATGG 6820 0.0 21.602066 2 GAGTACT 5420 0.0 20.836775 12-13 GCAGAGT 11735 0.0 20.41668 9 ACATGGG 6925 0.0 20.385239 3 GTACTTT 5770 0.0 19.532082 14-15 AGTACTT 5730 0.0 19.296286 12-13 CATGGGG 3870 0.0 18.728361 4 AGAGTAC 9960 0.0 18.611876 10-11 GTGTAGC 1020 0.0 17.683065 1 >>END_MODULE