FastQCFastQC Report
Thu 26 May 2016
SRR938907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938907_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832957
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58620.31981110304278826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52320.2854404113135223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31770.17332648829186936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56100.034.745431
GTATCAA81800.030.0630931
ATCAACG98350.024.7536983
TATCAAC101100.024.2681432
TCAACGC100550.024.2120974
CAACGCA101600.024.0085835
AACGCAG104050.023.443276
GTACATG66550.022.5579571
TACATGG69350.020.6660562
GAGTACT58250.020.41006912-13
ACGCAGA117550.020.2664747
CGCAGAG118000.019.7870128
ACATGGG70400.019.4140853
GTACTTT60950.018.99978814-15
CTAGACG1559.6867065E-418.3703174
GCAGAGT124550.018.1753859
AGTACTT61650.017.55231512-13
AGAGTAC104900.017.39592710-11
ATGGGCG1700.001799062716.7494095
CATGGGG39400.016.3809784
Produced by FastQC (version 0.11.5)