##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938907_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1832957 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11961655401627 33.0 31.0 34.0 30.0 34.0 2 32.37355158904437 34.0 31.0 34.0 30.0 34.0 3 32.45977947109507 34.0 31.0 34.0 30.0 34.0 4 36.02680968511536 37.0 35.0 37.0 35.0 37.0 5 35.946304796020854 37.0 35.0 37.0 35.0 37.0 6 35.98452009512498 37.0 35.0 37.0 35.0 37.0 7 35.9193996367618 37.0 35.0 37.0 35.0 37.0 8 35.88405183536766 37.0 35.0 37.0 35.0 37.0 9 37.55560386850319 39.0 37.0 39.0 35.0 39.0 10-11 37.45409957789517 39.0 37.0 39.0 34.0 39.0 12-13 37.350368557472976 39.0 37.0 39.0 34.0 39.0 14-15 38.65862838026206 40.0 38.0 41.0 34.0 41.0 16-17 38.74700688559524 40.0 38.0 41.0 34.0 41.0 18-19 38.77225979660189 40.0 38.0 41.0 34.0 41.0 20-21 38.74185673750121 40.0 38.0 41.0 34.0 41.0 22-23 38.66297954616502 40.0 38.0 41.0 34.0 41.0 24-25 38.575966866653175 40.0 38.0 41.0 34.0 41.0 26-27 38.41087788747909 40.0 38.0 41.0 34.0 41.0 28-29 38.23420789467511 40.0 38.0 41.0 33.5 41.0 30-31 38.029233364448814 40.0 37.5 41.0 33.0 41.0 32-33 37.94404342273168 40.0 37.0 41.0 33.0 41.0 34-35 37.84905456047251 40.0 37.0 41.0 33.0 41.0 36-37 37.71507814967836 40.0 37.0 41.0 33.0 41.0 38-39 37.565869248432996 40.0 37.0 41.0 32.0 41.0 40-41 37.379553639283415 40.0 37.0 41.0 32.0 41.0 42-43 37.18662167197594 40.0 36.0 41.0 31.0 41.0 44-45 36.92033610171979 39.5 36.0 41.0 31.0 41.0 46-47 36.59729879097 39.0 36.0 41.0 30.0 41.0 48-49 36.2506818217776 39.0 35.0 40.5 30.0 41.0 50-51 35.96532324544438 39.0 35.0 40.0 29.0 41.0 52-53 35.67699078592678 38.5 35.0 40.0 28.0 41.0 54-55 35.33100312773295 38.0 34.5 40.0 27.5 41.0 56-57 35.157505604332236 38.0 34.0 40.0 27.0 41.0 58-59 34.83745745262982 38.0 34.0 40.0 26.5 41.0 60-61 34.43891073276678 37.5 34.0 40.0 26.0 41.0 62-63 33.97781071787281 37.0 33.0 39.5 25.5 41.0 64-65 33.519313873702444 36.0 32.5 39.0 24.0 40.5 66-67 33.0242569792963 36.0 32.0 39.0 22.5 40.0 68-69 32.49031755791325 35.0 31.5 38.0 22.0 40.0 70-71 31.920272543218417 35.0 31.0 37.5 20.0 40.0 72-73 31.36577944818127 35.0 30.5 37.0 18.5 39.0 74-75 30.796168158882068 34.0 30.0 36.0 16.5 39.0 76-77 28.936635993097493 32.5 27.5 34.5 11.5 36.5 78-79 29.901617441107454 34.0 29.0 35.0 11.5 37.0 80-81 29.86204368133022 34.0 29.0 35.0 7.5 37.0 82-83 29.406238116878903 34.0 29.0 35.0 6.0 36.5 84-85 28.897608345422178 34.0 29.0 35.0 2.0 36.0 86-87 28.1184274917524 33.0 27.0 35.0 2.0 35.5 88-89 27.354233896376183 32.5 26.0 35.0 2.0 35.0 90-91 26.687500306881176 32.0 25.0 34.0 2.0 35.0 92-93 26.174489363362042 32.0 24.5 34.0 2.0 35.0 94-95 25.491946346804646 31.5 22.0 34.0 2.0 35.0 96-97 24.471189995182648 31.0 15.5 34.0 2.0 35.0 98-99 23.046374519424077 31.0 2.0 34.0 2.0 35.0 100-101 17.427993946393723 23.5 2.0 30.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05349643381632063 1101 2 0.10659569034885408 1101 3 0.13780107296350508 1101 4 0.06391433940044777 1101 5 0.10399287207287244 1101 6 0.03894844236164374 1101 7 0.17659557262236802 1101 8 0.10779742374507606 1101 9 0.1405594971811226 1101 10-11 0.1815360390994698 1101 12-13 0.2261200889034498 1101 14-15 0.2513111797471126 1101 16-17 0.19335169649729522 1101 18-19 0.20167876399418816 1101 20-21 0.1789460347287175 1101 22-23 0.3252946597283142 1101 24-25 0.11781677250552747 1101 26-27 0.1092685123077004 1101 28-29 0.14713061002395023 1101 30-31 0.17626925846204244 1101 32-33 0.27941924339579316 1101 34-35 0.403258368604412 1101 36-37 0.4844447130219578 1101 38-39 0.4137278496712895 1101 40-41 0.4021455593556098 1101 42-43 0.3788975293191683 1101 44-45 0.3766180400838337 1101 46-47 0.3648487236872313 1101 48-49 0.16950502408605672 1101 50-51 0.19324221505955563 1101 52-53 0.14220840798722634 1101 54-55 0.23575091248302016 1101 56-57 0.18544040817793928 1101 58-59 0.3000418016399422 1101 60-61 0.352644581192358 1101 62-63 0.520018835527047 1101 64-65 0.2689638559430989 1101 66-67 0.25675924047732934 1101 68-69 0.26278270849255847 1101 70-71 0.3450608751660269 1101 72-73 0.1152209551868495 1101 74-75 0.09551482871060557 1101 76-77 -0.1628700628569355 1101 78-79 0.1262013870801404 1101 80-81 0.2433010784778169 1101 82-83 0.26963872644647324 1101 84-85 0.6058451736360944 1101 86-87 0.7594626045156758 1101 88-89 0.7066512993265128 1101 90-91 0.5541264929206307 1101 92-93 0.3424945162057682 1101 94-95 0.1641836977833293 1101 96-97 0.5005190108641209 1101 98-99 0.448745166133115 1101 100-101 0.9993017162633322 1102 1 0.19814297374921352 1102 2 0.11556309326822145 1102 3 0.09791259280706299 1102 4 0.13654616229374028 1102 5 0.17361263068029587 1102 6 0.21211108314726346 1102 7 0.17806520620575128 1102 8 0.19289859170472567 1102 9 0.19703804006329761 1102 10-11 0.3003442767947604 1102 12-13 0.2750016847231578 1102 14-15 0.4504934477046234 1102 16-17 0.3923289303670572 1102 18-19 0.33189210630360577 1102 20-21 0.2849753726355786 1102 22-23 0.4231072732651029 1102 24-25 0.4083776242184669 1102 26-27 0.32002268378297316 1102 28-29 0.3497032869276211 1102 30-31 0.4573123877497238 1102 32-33 0.5003706883852104 1102 34-35 0.5227249527231521 1102 36-37 0.5551181932318343 1102 38-39 0.6032883754668532 1102 40-41 0.6455390538310439 1102 42-43 0.6615370192851984 1102 44-45 0.6020491793520648 1102 46-47 0.5653536952629636 1102 48-49 0.6826649399886833 1102 50-51 0.4784895449413682 1102 52-53 0.44908182515078465 1102 54-55 0.48109907639274496 1102 56-57 0.5461691674478857 1102 58-59 0.40729099218940945 1102 60-61 0.37038620752007034 1102 62-63 0.5661434909094893 1102 64-65 0.3307028255098885 1102 66-67 0.367903911728213 1102 68-69 0.24859962574159766 1102 70-71 0.4761466141436159 1102 72-73 0.6589514869664654 1102 74-75 0.628859248577676 1102 76-77 0.30470683494392503 1102 78-79 0.5858941565229827 1102 80-81 0.6786243444370825 1102 82-83 0.6209442659733071 1102 84-85 0.9349167031503178 1102 86-87 1.1642513192257695 1102 88-89 0.9635075857918594 1102 90-91 0.9816592983746695 1102 92-93 1.2419419927504052 1102 94-95 1.4799098123139025 1102 96-97 1.313618585301512 1102 98-99 1.4816464585825173 1102 100-101 1.5260072392271482 1103 1 0.17321745039977543 1103 2 0.06427283561926345 1103 3 0.10470909039160148 1103 4 0.08699402863834393 1103 5 0.08862168865130826 1103 6 0.0820557288799506 1103 7 0.15622643808980996 1103 8 0.10610913920874765 1103 9 0.1543287002887439 1103 10-11 0.12778590320377248 1103 12-13 0.14829274752508326 1103 14-15 0.18696285189141548 1103 16-17 0.08479068560054515 1103 18-19 0.2170491111345214 1103 20-21 0.11288801353735067 1103 22-23 0.18502353735528487 1103 24-25 0.21389946399302318 1103 26-27 0.09030800986993626 1103 28-29 0.12757868950411932 1103 30-31 0.1022565302617977 1103 32-33 0.23917956519681383 1103 34-35 0.20372283919657974 1103 36-37 0.19650154669398745 1103 38-39 0.11291448255219905 1103 40-41 0.4291219845878871 1103 42-43 0.31579124633832834 1103 44-45 0.2566018162280699 1103 46-47 0.3839537355206133 1103 48-49 0.4130322910353854 1103 50-51 0.4074171818173795 1103 52-53 0.3006684797401533 1103 54-55 0.6312515522381474 1103 56-57 0.5668565668037644 1103 58-59 0.5153012578898952 1103 60-61 0.37302258030590707 1103 62-63 0.4694828628901675 1103 64-65 0.4577730751073119 1103 66-67 0.35269708805671485 1103 68-69 0.3615575564984468 1103 70-71 0.4896555714705073 1103 72-73 0.6161520707023698 1103 74-75 0.6902340472894295 1103 76-77 0.453278694847306 1103 78-79 0.6659454850254001 1103 80-81 0.6755803629555999 1103 82-83 0.6888790485820024 1103 84-85 0.9535071822163417 1103 86-87 0.9282509166492794 1103 88-89 0.6985679722652911 1103 90-91 0.7225570439463311 1103 92-93 0.6824321296264095 1103 94-95 0.8443109797857211 1103 96-97 0.6762046360261493 1103 98-99 0.9963193651847746 1103 100-101 1.307264284315714 1104 1 0.03830012033241559 1104 2 -0.01901763182306837 1104 3 0.008330237564493359 1104 4 0.024891558746531928 1104 5 -0.045609787160081794 1104 6 0.020982040372089727 1104 7 0.03545076958603488 1104 8 0.07655208674906078 1104 9 0.06157507710033627 1104 10-11 0.06619782400393603 1104 12-13 0.06700677079513895 1104 14-15 0.16067993701083338 1104 16-17 0.09667882603736899 1104 18-19 0.06473639425352928 1104 20-21 0.1287279808776205 1104 22-23 0.07900190028649234 1104 24-25 0.12026811065560139 1104 26-27 0.12361276594260318 1104 28-29 0.17153459887337874 1104 30-31 -0.05308129378391868 1104 32-33 0.08676886913638526 1104 34-35 0.23448013660421907 1104 36-37 0.1889857271389701 1104 38-39 0.2094974586697731 1104 40-41 0.07887499540642295 1104 42-43 0.013337684386918625 1104 44-45 0.22817968926338494 1104 46-47 0.22976716683312048 1104 48-49 0.1445366796861265 1104 50-51 0.1441593741015197 1104 52-53 0.1808399433041501 1104 54-55 0.30117194415486637 1104 56-57 0.310820641104641 1104 58-59 0.43866520971679535 1104 60-61 0.17465964909545306 1104 62-63 0.15398582267787475 1104 64-65 0.21648301591156383 1104 66-67 0.08741437729941737 1104 68-69 -0.026811498410722834 1104 70-71 -0.03873389576770592 1104 72-73 0.09800551017981718 1104 74-75 0.06464106913062295 1104 76-77 -0.1323755202986412 1104 78-79 0.23384505636648711 1104 80-81 0.3307916305612366 1104 82-83 0.36971187160710883 1104 84-85 0.36576682704181707 1104 86-87 0.4199757584537309 1104 88-89 0.34247080957902654 1104 90-91 0.6092114632155656 1104 92-93 0.8492212702673569 1104 94-95 1.1148723188345073 1104 96-97 1.027953972625454 1104 98-99 1.0346782139294142 1104 100-101 1.1549434025031005 1105 1 0.05271829223398328 1105 2 0.13707399768060213 1105 3 0.1092507034119734 1105 4 0.024429240417418896 1105 5 0.07757286226723181 1105 6 0.08790334038171466 1105 7 0.12563781440620403 1105 8 0.13271077005619958 1105 9 0.1026684711777861 1105 10-11 0.1472633057325794 1105 12-13 0.21606881962571123 1105 14-15 0.20913577454171417 1105 16-17 0.10666021780483703 1105 18-19 0.17517545229195974 1105 20-21 0.1816761024086304 1105 22-23 0.1498431207298836 1105 24-25 0.08193402871521727 1105 26-27 0.11960862721502252 1105 28-29 0.31033693452738476 1105 30-31 0.20146537117163632 1105 32-33 0.26461352247785186 1105 34-35 0.37443377643866427 1105 36-37 0.33144393882366074 1105 38-39 0.2902775606942214 1105 40-41 0.22629919894198736 1105 42-43 0.4552957196142202 1105 44-45 0.3224568556787375 1105 46-47 0.4594535539485278 1105 48-49 0.37206451182704114 1105 50-51 0.25920236553530884 1105 52-53 0.29131124491793514 1105 54-55 0.3991202261016795 1105 56-57 0.3526039835282049 1105 58-59 0.20505184387253195 1105 60-61 0.34032986629843975 1105 62-63 0.343802627836844 1105 64-65 0.16432221511590228 1105 66-67 0.19430664204964643 1105 68-69 0.40238321428458335 1105 70-71 0.5332873432839165 1105 72-73 0.5313341379830838 1105 74-75 0.5498154411027727 1105 76-77 0.4693763806285638 1105 78-79 0.49914792139263753 1105 80-81 0.4936441442767858 1105 82-83 0.34631442542660906 1105 84-85 0.5684740040446066 1105 86-87 0.6956331423929001 1105 88-89 0.6023806559006921 1105 90-91 0.48842446331181577 1105 92-93 0.8597905859335597 1105 94-95 0.9950813406189702 1105 96-97 1.0941033518163188 1105 98-99 1.391725921587664 1105 100-101 1.2454994036580942 1106 1 0.1082204781338234 1106 2 0.15230803807374826 1106 3 0.06866371475073407 1106 4 0.09438977366276902 1106 5 0.12139872631632187 1106 6 0.1722127673523559 1106 7 0.16451193322524915 1106 8 0.15254408329659697 1106 9 0.10012660913375981 1106 10-11 0.24592162642851179 1106 12-13 0.17748737026942507 1106 14-15 0.11280516750240821 1106 16-17 0.09395059032785724 1106 18-19 0.17682788470039412 1106 20-21 0.16556897110870494 1106 22-23 0.22097686988121978 1106 24-25 0.38605884391308365 1106 26-27 0.25758506771983747 1106 28-29 0.22269313785097467 1106 30-31 0.1845325788670209 1106 32-33 0.17820988290703355 1106 34-35 0.3192280670840262 1106 36-37 0.3574615402348229 1106 38-39 0.5163733659238829 1106 40-41 0.5719833951082549 1106 42-43 0.5276591563944422 1106 44-45 0.3704018243020357 1106 46-47 0.465038068006173 1106 48-49 0.33285870095021153 1106 50-51 0.21092935330823082 1106 52-53 0.15221504628975424 1106 54-55 0.3303335432760619 1106 56-57 0.46298510420637484 1106 58-59 0.34586360902630275 1106 60-61 0.36626387052429976 1106 62-63 0.3634604980280258 1106 64-65 0.3673520182262848 1106 66-67 0.5237123882060857 1106 68-69 0.5396650208720999 1106 70-71 0.7033893730933727 1106 72-73 0.6543168601739353 1106 74-75 0.6844966931252863 1106 76-77 0.2548103245756188 1106 78-79 0.5020627188875757 1106 80-81 0.5237140676909746 1106 82-83 0.4464665500958702 1106 84-85 0.7536549356376803 1106 86-87 0.8369061990359441 1106 88-89 0.8465894489921055 1106 90-91 1.1219087051630723 1106 92-93 1.1190733633271073 1106 94-95 1.3230546720064602 1106 96-97 1.169629205078671 1106 98-99 1.2440752087114788 1106 100-101 1.3957782724469965 1107 1 0.001832033230449781 1107 2 0.08304392732091515 1107 3 0.041017861644156994 1107 4 0.09387688022032137 1107 5 0.05172838169767857 1107 6 0.10104300549691203 1107 7 0.1197445902235117 1107 8 0.09849859376005554 1107 9 0.1074796275997798 1107 10-11 0.049988447880380704 1107 12-13 0.06632536828463031 1107 14-15 0.1904454557615125 1107 16-17 0.19213598631312578 1107 18-19 0.08646264521099312 1107 20-21 0.11090052781295157 1107 22-23 0.21276485537551793 1107 24-25 0.19410406757358345 1107 26-27 0.23050698363499578 1107 28-29 0.1766927173958237 1107 30-31 0.1797282605453816 1107 32-33 0.09323914473001338 1107 34-35 0.19690545432631268 1107 36-37 0.1703447804600131 1107 38-39 0.2187577484885992 1107 40-41 0.045675733240756244 1107 42-43 0.03426138418839031 1107 44-45 0.011290004875149862 1107 46-47 0.05290546387396233 1107 48-49 0.07937371521785508 1107 50-51 -0.08090979786703656 1107 52-53 -0.20603035730081132 1107 54-55 -0.11212392428551965 1107 56-57 0.0842293731863677 1107 58-59 0.08921655329435652 1107 60-61 0.21203752344477067 1107 62-63 0.30786179772906763 1107 64-65 0.42559417565581725 1107 66-67 0.24898993497398436 1107 68-69 0.4439233658246309 1107 70-71 0.6383888629823531 1107 72-73 0.45239312331701953 1107 74-75 0.4594191717083511 1107 76-77 0.35331987292641287 1107 78-79 0.5130950290103407 1107 80-81 0.5789321942541577 1107 82-83 0.5257028675271371 1107 84-85 0.7776701895029312 1107 86-87 0.8242497263454283 1107 88-89 0.9761863994556883 1107 90-91 1.0657145661840985 1107 92-93 0.9674199853101086 1107 94-95 1.1701042207900798 1107 96-97 1.4858144093858634 1107 98-99 1.3494791393487446 1107 100-101 1.3011715371265993 1108 1 -0.05444627787354506 1108 2 0.017541424692424812 1108 3 0.08740643587693597 1108 4 0.009320837742386345 1108 5 -0.013869471267014433 1108 6 0.06495925993229434 1108 7 0.08701781216222315 1108 8 0.05788761423240629 1108 9 6.128777876241998E-4 1108 10-11 0.11247438370578777 1108 12-13 0.08166691954560434 1108 14-15 0.043587185772587134 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62-63 -0.45351920750321284 2316 64-65 -0.5558811691990968 2316 66-67 -0.6143629533511543 2316 68-69 -0.435781988015016 2316 70-71 -0.36650384881934883 2316 72-73 -0.14805082784835122 2316 74-75 -0.5356976297292029 2316 76-77 -0.3307792761671884 2316 78-79 -0.4195721133182886 2316 80-81 -0.429419637044397 2316 82-83 -0.6101271625981219 2316 84-85 -0.8624659026697898 2316 86-87 -0.9662314850070359 2316 88-89 -1.1981193983351268 2316 90-91 -1.2178777386623665 2316 92-93 -1.1525057585723495 2316 94-95 -1.0031424363633477 2316 96-97 -0.8847891527937257 2316 98-99 -1.1467034091423471 2316 100-101 -0.6710794009844925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 16.0 5 42.0 6 85.0 7 241.0 8 615.0 9 1136.0 10 1767.0 11 2780.0 12 4060.0 13 5559.0 14 6940.0 15 8920.0 16 11929.0 17 16571.0 18 16577.0 19 12822.0 20 12540.0 21 13768.0 22 14772.0 23 16505.0 24 18960.0 25 22041.0 26 25940.0 27 31599.0 28 37596.0 29 45779.0 30 54453.0 31 66060.0 32 81357.0 33 103076.0 34 136818.0 35 193796.0 36 288466.0 37 378946.0 38 193675.0 39 6745.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.92741433345947 19.780149972919883 15.157746474245412 23.134689219375236 2 15.882696648093763 20.643910359053706 39.46399179031477 24.00940120253776 3 19.210106947407933 24.41252031553386 30.084993810547655 26.29237892651055 4 13.58487951435849 17.270072347578257 36.085789246556246 33.05925889150701 5 14.09132892915655 37.04713203855846 33.98552175528395 14.876017277001042 6 32.377027939007846 35.82419009283906 17.08561630196453 14.71316566618857 7 28.580048522687658 32.70000332795587 20.562184492053003 18.157763657303473 8 26.144748622035323 35.53329401617169 19.404328634005054 18.917628727787942 9 26.31272855828042 16.944532795913926 20.265941863338856 36.476796782466806 10-11 25.14207916497768 26.60144236880625 26.896784812737014 21.35969365347905 12-13 25.982360742777928 23.854651254775753 27.576751664114322 22.586236338331997 14-15 22.87792831584825 25.178353833601523 25.848614537218413 26.09510331333181 16-17 22.477431484269488 28.40205388291951 26.536013467047226 22.584501165763783 18-19 22.350458196054554 27.025520077208913 28.65671658076684 21.967305145969693 20-21 23.056896588408783 26.156232797605185 28.772197056450317 22.01467355753572 22-23 22.872904274350134 26.10516776989313 28.576065886979347 22.44586206877739 24-25 22.750273328983347 26.349377726470248 28.575945085424852 22.32440385912155 26-27 22.610220155251913 26.491272433361075 28.47618042217587 22.422326989211147 28-29 22.38519507004256 26.526099630269556 28.724132644682882 22.364572655005 30-31 22.626826488564653 26.326340443338275 28.57808448316027 22.468748584936797 32-33 22.365063664886847 26.495002337752606 28.538449074364536 22.60148492299601 34-35 22.819580912555164 26.436234411352594 28.384542522032536 22.359642154059706 36-37 22.854434648604872 26.285713038410414 28.332152812186905 22.52769950079781 38-39 22.64527521860815 26.468957001858094 28.547647478146004 22.338120301387747 40-41 22.764561652759454 26.331878777562086 28.51622217057258 22.38733739910588 42-43 22.612502461984505 26.40049408823643 28.204276242026854 22.78272720775221 44-45 22.638231358765257 26.597842528298234 28.08281265131149 22.681113461625017 46-47 22.852949761176685 26.672758866196205 27.709860361331085 22.76443101129603 48-49 22.930279465363967 26.780440179703263 27.564248227068372 22.7250321278644 50-51 22.76199569000791 26.879620284241252 27.53037453285687 22.82800949289397 52-53 22.958612777059145 26.90284060127979 27.340712302579927 22.797834319081133 54-55 22.813955810201765 26.780442749066125 27.354051404370093 23.05155003636201 56-57 22.76780088130818 27.236672764281977 27.218805460248113 22.776720894161727 58-59 22.935551327998027 26.80748543193011 27.445501697539537 22.811461542532328 60-61 22.873365840351894 26.68197906366147 27.69420670372012 22.750448392266513 62-63 22.748886142226265 26.721702365854917 27.691787718198963 22.83762377371986 64-65 22.686580681274474 26.664846283062193 27.588413631163366 23.060159404499966 66-67 22.589637565203727 26.966968900256365 27.410553388084185 23.032840146455715 68-69 22.541860778506003 26.802082577481517 27.577866149168457 23.078190494844023 70-71 22.590355365674153 26.690287879093727 27.517966869926575 23.20138988530555 72-73 22.72475568166629 26.641677900790906 27.32860072549438 23.30496569204842 74-75 22.600366511598473 26.887864799883467 27.110865121222155 23.400903567295906 76-77 22.621648119162696 26.80145038286733 27.328976505948933 23.247924992021037 78-79 22.589544305475652 26.81000193408771 27.272573517838204 23.327880242598432 80-81 22.525882436060094 26.959251240417437 27.23329013342688 23.281576190095592 82-83 22.613105490199715 26.86309607917698 27.248920733001373 23.27487769762193 84-85 22.63682270605514 26.753697306427433 27.04208393436613 23.567396053151306 86-87 22.50628723296495 26.617977868612325 27.384759545249647 23.490975353173077 88-89 22.576139757381792 26.482063353384856 27.251933304786146 23.689863584447206 90-91 22.434668554240126 26.667962776102115 27.298282700635685 23.59908596902207 92-93 22.45156323907217 26.606325189297948 27.214959216173646 23.727152355456237 94-95 22.43492554853162 26.542208987860477 27.11336820265159 23.90949726095631 96-97 22.39736120378165 26.358119694024463 27.203365927296712 24.041153174897172 98-99 22.23685552907133 26.40787536205159 27.068965611304574 24.2863034975725 100-101 22.55630668107693 25.443041911058277 27.960236841861192 24.0404145660036 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 453.0 1 319.5 2 220.5 3 246.5 4 233.0 5 256.5 6 394.0 7 818.0 8 1377.5 9 1775.0 10 1768.0 11 1614.0 12 1665.0 13 1796.0 14 1940.5 15 2033.0 16 2149.5 17 2310.5 18 2435.0 19 2591.5 20 2941.5 21 3429.0 22 4073.0 23 4967.5 24 6016.5 25 7383.5 26 9264.5 27 11410.5 28 13520.0 29 15852.0 30 18602.5 31 21680.5 32 24591.5 33 27523.5 34 31094.0 35 34651.5 36 39004.0 37 43463.5 38 46320.5 39 47981.0 40 49511.0 41 52632.0 42 55509.5 43 58828.0 44 63353.5 45 65850.5 46 67104.5 47 69292.0 48 74559.5 49 75928.0 50 72542.5 51 70577.5 52 68215.0 53 67459.5 54 66477.0 55 62266.5 56 57203.0 57 51819.0 58 46452.5 59 41384.0 60 35559.0 61 28838.0 62 23094.5 63 18433.5 64 14249.0 65 10732.0 66 7645.5 67 5823.5 68 4194.5 69 2598.0 70 1737.5 71 1112.0 72 730.0 73 484.5 74 298.5 75 216.5 76 153.5 77 74.0 78 38.5 79 18.0 80 4.5 81 3.0 82 4.5 83 3.0 84 1.0 85 0.5 86 1.0 87 1.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37611356949453806 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.365148227699832E-4 16-17 0.002100431161232915 18-19 4.364532283081382E-4 20-21 0.0 22-23 0.0 24-25 5.455665353851727E-5 26-27 2.7278326769258637E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011620567203704179 36-37 0.023623030982177978 38-39 0.00919279612124016 40-41 0.00518288208615914 42-43 0.004691872204312486 44-45 0.0013366380116936732 46-47 6.546798424622072E-4 48-49 0.002536884389541053 50-51 3.818965747696209E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.455665353851728E-4 60-61 0.0010638547440010868 62-63 0.0013911946652321904 64-65 5.455665353851728E-4 66-67 0.002618719369848829 68-69 0.0019640395273866217 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.08611767761054952 78-79 0.0028096676572336395 80-81 3.00061594461845E-4 82-83 0.0 84-85 5.455665353851727E-5 86-87 0.09091866312193903 88-89 0.4314612945093639 90-91 0.3807781633720813 92-93 0.0 94-95 9.820197636933108E-4 96-97 0.0 98-99 0.0 100-101 2.182266141540691E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1832957.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.84795920145902 #Duplication Level Percentage of deduplicated Percentage of total 1 77.80686044613763 45.00968088681786 2 12.461001533470997 14.41687016635097 3 4.126690356019502 7.161618460562116 4 1.8247513395936645 4.22232564182488 5 0.9633106851206327 2.7862778605593945 6 0.6118846279396738 2.123776619583251 7 0.42052756997040114 1.7028663197515528 8 0.29344337413797006 1.3580080268057415 9 0.22684219640923042 1.1810122310745466 >10 1.1475914885283842 11.985568711870124 >50 0.08091774038409534 3.1970967166681583 >100 0.03485649735599507 3.606252449406781 >500 9.443892370679665E-4 0.3945996411759668 >1k 1.888778474135933E-4 0.24797006386511333 >5k 1.888778474135933E-4 0.6060762036835354 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5862 0.31981110304278826 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5232 0.2854404113135223 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3177 0.17332648829186936 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.455665353851727E-5 0.0 12-13 0.0 0.0 0.0 5.455665353851727E-5 1.636699606155518E-4 14-15 0.0 0.0 0.0 8.18349803077759E-5 3.00061594461845E-4 16-17 0.0 0.0 0.0 1.636699606155518E-4 3.818965747696209E-4 18-19 0.0 0.0 0.0 2.727832676925863E-4 3.818965747696209E-4 20-21 0.0 0.0 0.0 3.00061594461845E-4 3.818965747696209E-4 22-23 0.0 0.0 0.0 3.818965747696209E-4 3.818965747696209E-4 24-25 0.0 0.0 0.0 4.910098818466554E-4 3.818965747696209E-4 26-27 0.0 0.0 0.0 8.729064566162764E-4 4.364532283081382E-4 28-29 0.0 0.0 0.0 0.002755111003695122 4.364532283081382E-4 30-31 0.0 0.0 0.0 0.007174199940315021 4.364532283081382E-4 32-33 0.0 0.0 0.0 0.016448831041862955 4.364532283081382E-4 34-35 0.0 0.0 0.0 0.03235209554834074 4.910098818466554E-4 36-37 0.0 0.0 0.0 0.057966444384674604 4.910098818466554E-4 38-39 0.0 0.0 0.0 0.09255536272809456 4.910098818466554E-4 40-41 0.0 0.0 0.0 0.13066318522474885 4.910098818466554E-4 42-43 0.0 0.0 0.0 0.17196257195340642 4.910098818466554E-4 44-45 0.0 0.0 0.0 0.22646466883838517 4.910098818466554E-4 46-47 0.0 0.0 0.0 0.29138708654922074 5.455665353851728E-4 48-49 0.0 0.0 0.0 0.360183026661291 6.0012318892369E-4 50-51 0.0 0.0 0.0 0.4376261963592163 6.0012318892369E-4 52-53 0.0 0.0 0.0 0.5146601911556027 6.546798424622072E-4 54-55 0.0 0.0 0.0 0.6045422778603099 6.546798424622072E-4 56-57 0.0 0.0 0.0 0.7141738731459604 6.546798424622072E-4 58-59 0.0 0.0 0.0 0.8362171071116236 6.546798424622072E-4 60-61 0.0 0.0 0.0 0.9761822017646895 6.546798424622072E-4 62-63 0.0 0.0 0.0 1.1175930477365263 7.637931495392418E-4 64-65 0.0 0.0 0.0 1.2662053719754474 7.637931495392418E-4 66-67 0.0 0.0 0.0 1.43977736520824 7.637931495392418E-4 68-69 0.0 0.0 0.0 1.6408731901512148 7.637931495392418E-4 70-71 0.0 0.0 0.0 1.8658648293440598 7.637931495392418E-4 72-73 0.0 0.0 0.0 2.0996400897566065 8.183498030777591E-4 74-75 0.0 0.0 0.0 2.3473818534750133 9.00184783385535E-4 76-77 0.0 0.0 0.0 2.6207652443565235 9.820197636933108E-4 78-79 0.0 0.0 0.0 2.9219179718891386 9.820197636933108E-4 80-81 0.0 0.0 0.0 3.2545771668402477 0.0010092980904625693 82-83 0.0 5.455665353851727E-5 0.0 3.6071495403329155 0.001036576417231828 84-85 0.0 5.455665353851727E-5 0.0 3.9704695745726717 0.001036576417231828 86-87 0.0 5.455665353851727E-5 0.0 4.360440534065992 0.0010638547440010868 88-89 0.0 5.455665353851727E-5 0.0 4.779762973163036 0.0010911330707703453 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5610 0.0 34.74543 1 GTATCAA 8180 0.0 30.063093 1 ATCAACG 9835 0.0 24.753698 3 TATCAAC 10110 0.0 24.268143 2 TCAACGC 10055 0.0 24.212097 4 CAACGCA 10160 0.0 24.008583 5 AACGCAG 10405 0.0 23.44327 6 GTACATG 6655 0.0 22.557957 1 TACATGG 6935 0.0 20.666056 2 GAGTACT 5825 0.0 20.410069 12-13 ACGCAGA 11755 0.0 20.266474 7 CGCAGAG 11800 0.0 19.787012 8 ACATGGG 7040 0.0 19.414085 3 GTACTTT 6095 0.0 18.999788 14-15 CTAGACG 155 9.6867065E-4 18.370317 4 GCAGAGT 12455 0.0 18.175385 9 AGTACTT 6165 0.0 17.552315 12-13 AGAGTAC 10490 0.0 17.395927 10-11 ATGGGCG 170 0.0017990627 16.749409 5 CATGGGG 3940 0.0 16.380978 4 >>END_MODULE